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https://www.readbyqxmd.com/read/28931430/global-proteomic-profiling-of-yersinia-ruckeri-strains
#1
Gokhlesh Kumar, Karin Hummel, Timothy J Welch, Ebrahim Razzazi-Fazeli, Mansour El-Matbouli
Yersinia ruckeri is the causative agent of enteric redmouth disease (ERM) of salmonids. There is little information regarding the proteomics of Y. ruckeri. Herein, we perform whole protein identification and quantification of biotype 1 and biotype 2 strains of Y. ruckeri grown under standard culture conditions using a shotgun proteomic approach. Proteins were extracted, digested and peptides were separated by a nano liquid chromatography system and analyzed with a high-resolution hybrid triple quadrupole time of flight mass spectrometer coupled via a nano ESI interface...
September 20, 2017: Veterinary Research
https://www.readbyqxmd.com/read/28904995/distribution-of-o-acetylated-sialic-acids-among-target-host-tissues-for-influenza-virus
#2
Brian R Wasik, Karen N Barnard, Robert J Ossiboff, Zahra Khedri, Kurtis H Feng, Hai Yu, Xi Chen, Daniel R Perez, Ajit Varki, Colin R Parrish
Sialic acids (Sias) are important glycans displayed on the cells and tissues of many different animals and are frequent targets for binding and modification by pathogens, including influenza viruses. Influenza virus hemagglutinins bind Sias during the infection of their normal hosts, while the encoded neuraminidases and/or esterases remove or modify the Sia to allow virion release or to prevent rebinding. Sias naturally occur in a variety of modified forms, and modified Sias can alter influenza virus host tropisms through their altered interactions with the viral glycoproteins...
September 2017: MSphere
https://www.readbyqxmd.com/read/28877472/alpk1-and-tifa-dependent-innate-immune-response-triggered-by-the-helicobacter-pylori-type-iv-secretion-system
#3
Stephanie Zimmermann, Lennart Pfannkuch, Munir A Al-Zeer, Sina Bartfeld, Manuel Koch, Jianping Liu, Cindy Rechner, Meike Soerensen, Olga Sokolova, Alla Zamyatina, Paul Kosma, André P Mäurer, Frithjof Glowinski, Klaus-Peter Pleissner, Monika Schmid, Volker Brinkmann, Alexander Karlas, Michael Naumann, Marion Rother, Nikolaus Machuy, Thomas F Meyer
Activation of transcription factor NF-κB is a hallmark of infection with the gastric pathogen Helicobacter pylori, associated with inflammation and carcinogenesis. Genome-wide RNAi screening revealed numerous host factors involved in H. pylori-, but not IL-1β- and TNF-α-dependent NF-κB regulation. Pathway analysis including CRISPR/Cas9-knockout and recombinant protein technology, immunofluorescence microscopy, immunoblotting, mass spectrometry, and mutant H. pylori strains identified the H. pylori metabolite D-glycero-β-D-manno-heptose 1,7-bisphosphate (βHBP) as a cagPAI type IV secretion system (T4SS)-dependent effector of NF-κB activation in infected cells...
September 5, 2017: Cell Reports
https://www.readbyqxmd.com/read/28875943/molecular-mechanisms-used-by-salmonella-to-evade-the-immune-system
#4
Joaquín Bernal-Bayard, Francisco Ramos-Morales
Human and animal pathogens are able to circumvent, at least temporarily, the sophisticated immune defenses of their hosts. Several serovars of the Gram-negative bacterium Salmonella enterica have been used as models for the study of pathogen-host interactions. In this review we discuss the strategies used by Salmonella to evade or manipulate three levels of host immune defenses: physical barriers, innate immunity and adaptive immunity. During its passage through the digestive system, Salmonella has to face the acidic pH of the stomach, bile and antimicrobial peptides in the intestine, as well as the competition with resident microbiota...
September 6, 2017: Current Issues in Molecular Biology
https://www.readbyqxmd.com/read/28874682/hydrogen-peroxide-dynamics-in-subcellular-compartments-of-malaria-parasites-using-genetically-encoded-redox-probes
#5
Mahsa Rahbari, Stefan Rahlfs, Jude M Przyborski, Anna Katharina Schuh, Nicholas H Hunt, David A Fidock, Georges E Grau, Katja Becker
Redox balance is essential for the survival, growth and multiplication of malaria parasites and oxidative stress is involved in the mechanism of action of many antimalarial drugs. Hydrogen peroxide (H2O2) plays an important role in redox signalling and pathogen-host cell interactions. For monitoring intra- and subcellular redox events, highly sensitive and specific probes are required. Here, we stably expressed the ratiometric H2O2 redox sensor roGFP2-Orp1 in the cytosol and the mitochondria of Plasmodium falciparum (P...
September 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28860554/transcriptome-analysis-reveals-the-host-selection-fitness-mechanisms-of-the-rhizoctonia-solani-ag1ia-pathogen
#6
Yuan Xia, Binghong Fei, Jiayu He, Menglin Zhou, Danhua Zhang, Linxiu Pan, Shuangcheng Li, Yueyang Liang, Lingxia Wang, Jianqing Zhu, Ping Li, Aiping Zheng
Rhizoctonia solani AG1IA is a major generalist pathogen that causes sheath blight. Its genome, which was the first to be sequenced from the Rhizoctonia genus, may serve as a model for studying pathogenic mechanisms. To explore the pathogen-host fitness mechanism of sheath-blight fungus, a comprehensive comparative transcriptome ecotype analysis of R. solani AG1IA isolated from rice, soybean and corn during infection was performed. Special characteristics in gene expression, gene ontology terms and expression of pathogenesis-associated genes, including genes encoding secreted proteins, candidate effectors, hydrolases, and proteins involved in secondary metabolite production and the MAPK pathway, were revealed...
August 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28858701/systems-level-interference-strategies-to-decipher-host-factors-involved-in-bacterial-pathogen-interaction-from-rnai-to-crispri
#7
REVIEW
Maxime Québatte, Christoph Dehio
Bacterial pathogen-host cell interactions involve an intricate interplay of multiple components from both partners. Systems level surveys have been used widely to profile host requirements for pathogen infection. Functional genomics, and more specifically genome-wide perturbation screens, constitute attractive methodologies to assess such host infectomes. Although these strategies have successfully identified numerous critical host factors, they may have failed in generating the high-quality data required for systems level analysis...
August 28, 2017: Current Opinion in Microbiology
https://www.readbyqxmd.com/read/28835201/irak-m-alters-the-polarity-of-macrophages-to-facilitate-the-survival-of-mycobacterium-tuberculosis
#8
Pei Shen, Quan Li, Jilei Ma, Maopeng Tian, Fei Hong, Xinjie Zhai, Jianrong Li, Hanju Huang, Chunwei Shi
BACKGROUND: Intracellular bacterium, Mycobacterium tuberculosis (M. tb), infects specifically macrophages as host cells. IRAK-M, a member of IRAK family, is a negative regulator in TLR signaling and specifically expresses in monocytes and macrophages. The role of IRAK-M in intracellular growth of M. tb and macrophage polarization was explored, for deeply understanding the pathogenesis of M. tb, the significance of IRAK-M to innate immunity and pathogen-host interaction. METHODS: IRAK-M expression was detected in M...
August 23, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28830681/cloning-and-characterization-of-an-insect-apolipoprotein-apolipophorin-ii-i-involved-in-the-host-immune-response-of-antheraea-pernyi
#9
Daihua Wen, Hao Luo, Tienan Li, Chunfu Wu, Jinghai Zhang, Xialu Wang, Rong Zhang
Apolipoproteins are protein components of lipoprotein particles, and are increasingly recognized to be functioning in the innate immune systems of both insects and mammals. Mammalian apolipoprotein B (apoB) is associated with a diverse range of innate immune defenses including suppression of bacterial pathogenesis, virus toxicity neutralization, and inhibition of cytokine releases from immune cells. However, little is known about apoB homologous insect apolipophorin-II/I (apoLp-II/I) in controlling of specific pathogen-host encounters such as microbial infections...
August 19, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28783440/functional-and-structural-characteristics-of-bacterial-proteins-that-bind-host-cytokines
#10
Martin Högbom, Riikka Ihalin
Several human pathogens bind and respond to host cytokines, which can be considered a virulence mechanism that communicates defensive actions of the host to the pathogen. This review summarizes the current knowledge of bacterial cytokine-binding proteins, with a particular focus on their functional and structural characteristics. Many bacterial cytokine-binding proteins function in the development of infection and inflammation and mediate adhesion to host cells, suggesting multiple roles in pathogen-host interactions...
August 7, 2017: Virulence
https://www.readbyqxmd.com/read/28768558/chlamydia-gallinacea-a-widespread-emerging-chlamydia-agent-with-zoonotic-potential-in-backyard-poultry
#11
L Li, M Luther, K Macklin, D Pugh, J Li, J Zhang, J Roberts, B Kaltenboeck, C Wang
Chlamydia gallinacea, a new chlamydial agent, has been reported in four European countries as well as Argentina and China. Experimentally infected chickens with C. gallinacea in previous study showed no clinical signs but had significantly reduced gains in body weight (6·5-11·4%). Slaughterhouse workers exposed to infected chickens have developed atypical pneumonia, indicating C. gallinacea is likely a zoonotic agent. In this study, FRET-PCR confirmed that C. gallinacea was present in 12·4% (66/531) of oral-pharyngeal samples from Alabama backyard poultry...
August 3, 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/28766182/lipidomics-and-anti-trypanosomatid-chemotherapy
#12
REVIEW
Michael Biagiotti, Sedelia Dominguez, Nader Yamout, Rachel Zufferey
BACKGROUND: Trypanosomatids such as Leishmania, Trypanosoma brucei and Trypanosoma cruzi belong to the order Kinetoplastida and are the source of many significant human and animal diseases. Current treatment is unsatisfactory and is compromised by the rising appearance of drug resistant parasites. Novel and more effective chemotherapeutics are urgently needed to treat and prevent these devastating diseases, which relies on the identification of essential, parasite specific targets that are absent in the host...
December 2017: Clinical and Translational Medicine
https://www.readbyqxmd.com/read/28756939/bacteriology-of-peritonsillar-abscess-the-changing-trend-and-predisposing-factors
#13
Yi-Wen Tsai, Yu-Hsi Liu, Hsing-Hao Su
INTRODUCTION: Peritonsillar abscess (PTA) is the most common deep neck infection. The infectious microorganism may be different according to clinical factors. OBJECTIVE: To identify the major causative pathogen of peritonsillar abscess and investigate the relationship between the causative pathogen, host clinical factors, and hospitalization duration. METHODS: This retrospective study included 415 hospitalized patients diagnosed with peritonsillar abscess who were admitted to a tertiary medical center from June 1990 to June 2013...
July 17, 2017: Brazilian Journal of Otorhinolaryngology
https://www.readbyqxmd.com/read/28752081/absence-of-nucleotide-oligomerization-domain-2-is-associated-with-less-distinct-disease-in-campylobacter-jejuni-infected-secondary-abiotic-il-10-deficient-mice
#14
Markus M Heimesaat, Ursula Grundmann, Marie E Alutis, André Fischer, Stefan Bereswill
Human Campylobacter jejuni-infections are progressively increasing worldwide. Despite their high prevalence and socioeconomic impact the underlying mechanisms of pathogen-host-interactions are only incompletely understood. Given that the innate immune receptor nucleotide-oligomerization-domain-2 (Nod2) is involved in clearance of enteropathogens, we here evaluated its role in murine campylobacteriosis. To address this, we applied Nod2-deficient IL-10(-/-) (Nod2(-/-) IL-10(-/-)) mice and IL-10(-/-) counterparts both with a depleted intestinal microbiota to warrant pathogen-induced enterocolitis...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28740179/legionella-pneumophila-infection-activates-bystander-cells-differentially-by-bacterial-and-host-cell-vesicles
#15
Anna Lena Jung, Christina Elena Herkt, Christine Schulz, Kathrin Bolte, Kerstin Seidel, Nicoletta Scheller, Alexandra Sittka-Stark, Wilhelm Bertrams, Bernd Schmeck
Extracellular vesicles from eukaryotic cells and outer membrane vesicles (OMVs) released from gram-negative bacteria have been described as mediators of pathogen-host interaction and intercellular communication. Legionella pneumophila (L. pneumophila) is a causative agent of severe pneumonia. The differential effect of bacterial and host cell vesicles in L. pneumophila infection is unknown so far. We infected THP-1-derived or primary human macrophages with L. pneumophila and isolated supernatant vesicles by differential centrifugation...
July 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28730142/taming-parasites-by-tailoring-them
#16
Bingjian Ren, Nishith Gupta
The next-generation gene editing based on CRISPR (clustered regularly interspaced short palindromic repeats) has been successfully implemented in a wide range of organisms including some protozoan parasites. However, application of such a versatile game-changing technology in molecular parasitology remains fairly underexplored. Here, we briefly introduce state-of-the-art in human and mouse research and usher new directions to drive the parasitology research in the years to come. In precise, we outline contemporary ways to embolden existing apicomplexan and kinetoplastid parasite models by commissioning front-line gene-tailoring methods, and illustrate how we can break the enduring gridlock of gene manipulation in non-model parasitic protists to tackle intriguing questions that remain long unresolved otherwise...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28723967/epitope-determination-of-immunogenic-proteins-of-neisseria-gonorrhoeae
#17
Daniel O Connor, Lena Danckert, Sebastian Hoppe, Frank F Bier, Markus von Nickisch-Rosenegk
Neisseria gonorrhoeae is the causative organism of gonorrhoea, a sexually transmitted disease that globally accounts for an estimated 80 to 100 million new infections per year. Increasing resistances to all common antibiotics used for N. gonorrhoeae treatment pose the risk of an untreatable disease. Further knowledge of ways of infection and host immune response are needed to understand the pathogen-host interaction and to discover new treatment alternatives against this disease. Therefore, detailed information about immunogenic proteins and their properties like epitope sites could advance further research in this area...
2017: PloS One
https://www.readbyqxmd.com/read/28719319/phosphoproteome-of-toxoplasma-gondii-infected-host-cells-reveals-specific-cellular-processes-predominating-in-different-phases-of-infection
#18
Cheng He, Ai-Yuan Chen, Hai-Xia Wei, Xiao-Shuang Feng, Hong-Juan Peng
The invasion of Toxoplasma gondii tachyzoites into the host cell results in extensive host cell signaling activation/deactivation that is usually regulated by the phosphorylation/dephosphorylation. To elucidate how T. gondii regulates host cell signal transduction, the comparative phosphoproteome of stable isotope labeling with amino acids in cell culture-labeled human foreskin fibroblast cells was analyzed. The cells were grouped (Light [L], Medium [M], and Heavy [H] groups) based on the labeling isotope weight and were infected with T...
July 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28695265/conservation-of-erwinia-amylovora-pathogenicity-relevant-genes-among-erwinia-genomes
#19
Luigimaria Borruso, Marco Salomone-Stagni, Ivan Polsinelli, Armin Otto Schmitt, Stefano Benini
The Erwinia genus comprises species that are plant pathogens, non-pathogen, epiphytes, and opportunistic human pathogens. Within the genus, Erwinia amylovora ranks among the top 10 plant pathogenic bacteria. It causes the fire blight disease and is a global threat to commercial apple and pear production. We analyzed the presence/absence of the E. amylovora genes reported to be important for pathogenicity towards Rosaceae within various Erwinia strains genomes. This simple bottom-up approach, allowed us to correlate the analyzed genes to pathogenicity, host specificity, and make useful considerations to drive targeted studies...
July 10, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28667627/bacterial-filamentous-appendages-investigated-by-solid-state-nmr-spectroscopy
#20
Birgit Habenstein, Antoine Loquet
The assembly of filamentous appendages at the surface of bacteria is essential in many infection mechanisms. The extent of mechanical, dynamical, and functional properties of such appendages is very diverse, ranging from a structural scaffold of the pathogen-host cell interaction to cell motility, surface adhesion, or the export of virulence effectors. In particular, the architectures of several bacterial secretion systems have revealed the presence of filamentous architectures, known as pili, fimbriae, andneedles...
2017: Methods in Molecular Biology
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