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https://www.readbyqxmd.com/read/28454463/reduced-ebp50-expression-levels-are-correlated-with-unfavorable-clinicopathological-features-of-extrahepatic-bile-duct-carcinoma-and-promote-the-proliferation-and-migration-of-qbc939-cells
#1
Duiping Feng, Ying Xiong, Zhiqiang Peng, Qiang Ma, Tao Tao, Hua Liu, Jianfang Liang, Zhigang Wei, Junfang Zheng, Lei Wang, Hui Zhang
The present study aimed to clarify the association between ezrin-radixin-moesin-binding phosphoprotein-50 (EBP50) expression level and the tumor phenotype and clinicopathological features of extrahepatic bile duct carcinoma. Tissue samples from patients with extrahepatic bile duct carcinoma (54 cases) and patients with normal bile duct epithelia from gallbladder of cholecystitis (20 cases) were collected, and immunohistochemical staining was used to detect the expression levels of EBP50 in these tissues. In addition, small interfering (si)RNA-EBP50 was used to knock down the expression of EBP50 in the QBC939 human cholangiocarcinoma (CC) cell line...
April 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454403/mir-22-promotes-apoptosis-of-osteosarcoma-cells-via-inducing-cell-cycle-arrest
#2
Pengzhou Gai, Hongliang Sun, Guangda Wang, Qiang Xu, Xiaojun Qi, Zuofu Zhang, Lei Jiang
To study the effects of miR-22 on the proliferation and the apoptosis of osteosarcoma MG-63 cell line and to explore the potential molecular mechanism that miR-22 regulates this biological process. Quantitive real-time polymerase chain reaction (RT-qPCR) was performed to explore the miRNA level of miR-22. The MG-63 cell line was infected with miR-22 mimics for establishment of miR-22 overexpression. Non-infected cells were in blank group and cells infected with empty vector were served as negative control (NC group)...
April 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454319/construction-and-expression-of-a-lentivirus-expression-vector-carrying-the-vegf165-egfp-fusion-gene-in-breast-cancer-mcf-7-cells
#3
Minna Luo, Huan Huang, Lei Hou, Shan Shao, Shangke Huang, Xinhan Zhao
Vascular endothelial growth factor (VEGF)165 is one of the most abundant and potent angiogenic factors in both physiological and pathological conditions. However, the function and mechanism of VEGF165 in tumors and their environment remain to be elucidated. In the present study, a lentivirus vector (LV) that contained the VEGF165-enhanced green fluorescent protein (EGFP) fusion gene was constructed and transfected into the human breast cancer cell line MCF-7. Following transfection, the expression of VEGF165 in MCF-7 cells was detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blotting...
March 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454290/a-multi-shrna-vector-enhances-the-silencing-efficiency-of-exogenous-and-endogenous-genes-in-human-cells
#4
Yanjie Weng, Ying Shi, Xi Xia, Wenjuan Zhou, Hongyan Wang, Changyu Wang
RNA interference (RNAi) is a powerful technology for suppressing gene function. In most studies, small interfering RNAs (siRNAs) consist of one short hairpin RNA (shRNA) and, therefore, are often unable to achieve loss-of-function of their target genes. In the current study, an RNAi vector containing three shRNAs under the control of three RNA polymerase III U6 promoters was constructed. RNAi vectors containing one or two shRNAs were generated for comparisons. A pilot study targeting exogenously expressed DsRed in the HEK293 cell line revealed promising effects and a high selectivity for the multi-shRNA RNAi vector...
March 2017: Oncology Letters
https://www.readbyqxmd.com/read/28453911/validation-of-biomechanical-deformable-image-registration-in-the-abdomen-thorax-and-pelvis-in-a-commercial-radiotherapy-treatment-planning-system
#5
Michael Velec, Joanne L Moseley, Stina Svensson, Björn Hårdemark, David A Jaffray, Kristy K Brock
PURPOSE: The accuracy of deformable image registration tools can vary widely between imaging modalities and specific implementations of the same algorithms. A biomechanical model-based algorithm initially developed in-house at an academic institution was translated into a commercial radiotherapy treatment planning system and validated for multiple imaging modalities and anatomic sites. METHODS: Biomechanical deformable registration (Morfeus) is a geometry driven algorithm based on the finite element method...
April 28, 2017: Medical Physics
https://www.readbyqxmd.com/read/28453776/modulating-and-evaluating-receptor-promiscuity-through-directed-evolution-and-modeling
#6
Sarah C Stainbrook, Jessica S Yu, Michael P Reddick, Neda Bagheri, Keith E J Tyo
The promiscuity of G-protein-coupled receptors (GPCRs) has broad implications in disease, pharmacology and biosensing. Promiscuity is a particularly crucial consideration for protein engineering, where the ability to modulate and model promiscuity is essential for developing desirable proteins. Here, we present methodologies for (i) modifying GPCR promiscuity using directed evolution and (ii) predicting receptor response and identifying important peptide features using quantitative structure-activity relationship models and grouping-exhaustive feature selection...
April 27, 2017: Protein Engineering, Design & Selection: PEDS
https://www.readbyqxmd.com/read/28453513/genetic-diversity-and-population-structure-of-the-tsetse-fly-glossina-fuscipes-fuscipes-diptera-glossinidae-in-northern-uganda-implications-for-vector-control
#7
Robert Opiro, Norah P Saarman, Richard Echodu, Elizabeth A Opiyo, Kirstin Dion, Alexis Halyard, Augustine W Dunn, Serap Aksoy, Adalgisa Caccone
Uganda is the only country where the chronic and acute forms of human African Trypanosomiasis (HAT) or sleeping sickness both occur and are separated by < 100 km in areas north of Lake Kyoga. In Uganda, Glossina fuscipes fuscipes is the main vector of the Trypanosoma parasites responsible for these diseases as well for the animal African Trypanosomiasis (AAT), or Nagana. We used highly polymorphic microsatellite loci and a mitochondrial DNA (mtDNA) marker to provide fine scale spatial resolution of genetic structure of G...
April 28, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28448995/cytoprotective-effect-of-heat-shock-protein-27-against-lipopolysaccharide-induced-apoptosis-of-renal-epithelial-hk-2-cells
#8
Chunmei Li, Jiang Wu, Yuan Li, Guangqun Xing
BACKGROUND: In response to various stimuli, heat shock protein 27 (Hsp27) functions as an anti-apoptotic or/and anti-inflammatory factor which confers a survival advantage to cells. This study was aimed to explore whether Hsp27 also has a cytoprotective role in human renal tubular epithelial cells, and to evaluate its potential in treating septic acute kidney injury (septic AKI). METHODS: HK-2 cells were subjected to different concentrations (0-10 µg/mL) of lipopolysaccharide (LPS) for various times (0-24 h) to establish a septic AKI model in vitro...
April 25, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28448507/detecting-the-impact-of-temperature-on-transmission-of-zika-dengue-and-chikungunya-using-mechanistic-models
#9
Erin A Mordecai, Jeremy M Cohen, Michelle V Evans, Prithvi Gudapati, Leah R Johnson, Catherine A Lippi, Kerri Miazgowicz, Courtney C Murdock, Jason R Rohr, Sadie J Ryan, Van Savage, Marta S Shocket, Anna Stewart Ibarra, Matthew B Thomas, Daniel P Weikel
Recent epidemics of Zika, dengue, and chikungunya have heightened the need to understand the seasonal and geographic range of transmission by Aedes aegypti and Ae. albopictus mosquitoes. We use mechanistic transmission models to derive predictions for how the probability and magnitude of transmission for Zika, chikungunya, and dengue change with mean temperature, and we show that these predictions are well matched by human case data. Across all three viruses, models and human case data both show that transmission occurs between 18-34°C with maximal transmission occurring in a range from 26-29°C...
April 27, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28447889/bone-marrow-as-a-hematopoietic-stem-cell-source-for-gene-therapy-in-sickle-cell-disease-scd-evidence-from-rhesus-and-scd-patients
#10
Naoya Uchida, Atsushi Fujita, Matthew M Hsieh, Aylin Bonifacino, Allen E Krouse, Mark E Metzger, Robert E Donahue, John F Tisdale
Steady state bone marrow (BM) is the preferred hematopoietic stem cell (HSC) source for gene therapy in sickle cell disease (SCD) due to the recognized risk of vaso-occlusive crisis during granulocyte colony-stimulating factor mobilization. We previously established clinically relevant HSC gene transfer in the rhesus model following transplantation of mobilized peripheral blood (PB) CD34+ cells transduced with lentiviral vectors. In this study, we examined steady state bone marrow (BM) in the rhesus competitive repopulation model and demonstrate similar gene marking in vitro and in vivo, as compared to mobilized PB CD34+ cells...
April 27, 2017: Human Gene Therapy. Clinical Development
https://www.readbyqxmd.com/read/28446879/the-mammalian-peptide-adrenomedullin-acts-as-a-growth-factor-in-tobacco-plants
#11
Rafael Peláez, María Niculcea, Alfredo Martínez
Growth factors are extracellular signals that regulate cell proliferation and total body mass. Some animal growth factors can work on plant tissues and vice versa. Here we show that the mammalian growth factor adrenomedullin (AM) induces growth in tobacco plants. Addition of synthetic AM resulted in a dose-dependent growth of tobacco calluses. Furthermore, AM transgenic plants showed enhanced survival and significant increases in stem diameter, plant height, leaf length, weight of all organs, and a reduction in the time to flowering when compared to plants transformed with the control vector...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28446872/a-neurocomputational-model-of-goal-directed-navigation-in-insect-inspired-artificial-agents
#12
Dennis Goldschmidt, Poramate Manoonpong, Sakyasingha Dasgupta
Despite their small size, insect brains are able to produce robust and efficient navigation in complex environments. Specifically in social insects, such as ants and bees, these navigational capabilities are guided by orientation directing vectors generated by a process called path integration. During this process, they integrate compass and odometric cues to estimate their current location as a vector, called the home vector for guiding them back home on a straight path. They further acquire and retrieve path integration-based vector memories globally to the nest or based on visual landmarks...
2017: Frontiers in Neurorobotics
https://www.readbyqxmd.com/read/28446674/broadening-cd4-and-cd8-t-cell-responses-against-hepatitis-c-virus-by-vaccination-with-ns3-overlapping-peptide-panels-in-cross-priming-liposomes
#13
Jonathan Filskov, Marianne Mikkelsen, Paul R Hansen, Jan P Christensen, Allan R Thomsen, Peter Andersen, Jens Bukh, Else Marie Agger
Despite the introduction of effective drugs to treat patients with chronic hepatitis C virus (HCV) infection, a vaccine would be the only means to substantially reduce the worldwide disease burden. An incomplete understanding of how HCV interacts with its human host and evades immune surveillance has hampered vaccine development. It is generally accepted that in infected individuals, a narrow repertoire of exhausted T cells is a hallmark of persistent infection, whereas broad vigorous CD4(+) and CD8(+) T cell responses are associated with control of acute hepatitis C...
April 26, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28446607/juvenile-hormone-and-its-receptor-methoprene-tolerant-promote-ribosomal-biogenesis-and-vitellogenesis-in-the-aedes-aegypti-mosquito
#14
Jia-Lin Wang, Tusar T Saha, Yang Zhang, Changyu Zhang, Alexander S Raikhel
Juvenile hormone (JH) controls many biological activities in insects, including development, metamorphosis and reproduction. In the Aedes aegypti mosquito, a vector of Dengue, Yellow fever, Chikungunya and Zika viruses, the metabolic tissue, the fat body, which is an analogue of the vertebrate liver, produces yolk proteins (YPs) for developing oocytes. JH is important for the fat body to acquire competence for YP production. However, the molecular mechanisms of how JH promotes mosquito reproduction are not completely understood...
April 26, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28445362/effect-of-human-fat-graft-on-breast-cancer-metastasis-in-a-murine-model
#15
Roberto Moltó-García, Virina González-Alonso, María Eloísa Villaverde-Doménech, Edurne Novella-Maestre
BACKGROUND: Isolated adipose stem cells have been reported to encourage migration and early metastasis of breast cancer. Mimicking a surgical situation, the authors developed a human breast cancer model to evaluate in vivo whether human adipose tissue promotes tumor growth and invasion. METHODS: Human adipose tissue was obtained from four patients. The MDA-MB-468 cell line was cultured with a lentiviral vector encoding a puromycin resistance gene and mCherry fluorescent protein...
May 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28445281/relationship-between-the-abnormal-diastolic-vortex-structure-and-impaired-left-ventricle-filling-in-patients-with-hyperthyroidism
#16
Bin-Yu Zhou, Ming-Xing Xie, Jing Wang, Xin-Fang Wang, Qing Lv, Man-Wei Liu, Shuang-Shuang Kong, Ping-Yu Zhang, Jin-Feng Liu
Intraventricular hydrodynamics plays an important role in evaluating cardiac function. Relationship between diastolic vortex and left ventricular (LV) filling is still rarely elucidated. The aim of this study was to evaluate the evolution of vortex during diastole in hyperthyroidism (HT) and explore the alteration of hydromechanics characteristics with sensitive indexes.Forty-three patients diagnosed with HT were classified into 2 groups according to whether myocardial damage existed: simple hyperthyroid group (HT1, n = 21) and thyrotoxic cardiomyopathy (HT2, n = 22)...
April 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28444748/identification-of-coagulation-gene-3-utr-variants-that-are-potentially-regulated-by-micrornas
#17
Carla Y Vossen, Astrid van Hylckama Vlieg, Raúl Teruel-Montoya, Salam Salloum-Asfar, Hugoline de Haan, Javier Corral, Pieter Reitsma, Bobby P C Koeleman, Constantino Martínez
MicroRNAs have been recognized as critical regulators of gene expression and might affect the risk of venous thrombosis. We aimed to identify 3' untranslated region (UTR) variants in coagulation genes that influence coagulation factor levels and venous thrombosis risk. The 3'UTR of coagulation genes were sequenced in subjects with extremely high or low plasma levels of these factors in two case-control studies. In total, 28 variants were identified. Five single nucleotide polymorphisms (SNPs) were predominantly present in one extreme level group (F2 rs1799963, F8 rs1050705 and F11 rs4253429, rs4253430 and rs1062547)...
April 26, 2017: British Journal of Haematology
https://www.readbyqxmd.com/read/28444003/species-abundance-and-temporal-variation-of-arbovirus-vectors-in-brownsville-texas
#18
Krithika Srinivasan, Beatriz Tapia, Arturo Rodriguez, Robert Wood, Jennifer J Salinas
The recent outbreaks of the dengue fever and West Nile viruses and the looming threats of the Zika and chikungunya viruses highlight the importance of establishing effective, proactive arboviral surveillance in communities at high risk of transmission, such as those on the Texas-Mexico border. Currently, there are no approved human vaccines available for these mosquito-borne diseases, so entomological control and case management are the only known methods for decreasing disease incidence. The principal vectors, which include Culex quinquefasciatus, Aedes aegypti, and Ae...
April 20, 2017: Revista Panamericana de Salud Pública, Pan American Journal of Public Health
https://www.readbyqxmd.com/read/28443981/mapping-the-dengue-scientific-landscape-worldwide-a-bibliometric-and-network-analysis
#19
Fabio Batista Mota, Bruna de Paula Fonseca E Fonseca, Andréia Cristina Galina, Roseli Monteiro da Silva
BACKGROUND: Despite the current global trend of reduction in the morbidity and mortality of neglected diseases, dengue's incidence has increased and occurrence areas have expanded. Dengue also persists as a scientific and technological challenge since there is no effective treatment, vaccine, vector control or public health intervention. Combining bibliometrics and social network analysis methods can support the mapping of dengue research and development (R&D) activities worldwide. OBJECTIVES: The aim of this paper is to map the scientific scenario related to dengue research worldwide...
May 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28443980/chagas-disease-national-survey-of-seroprevalence-in-children-under-five-years-of-age-conducted-in-2008
#20
Graciela Russomando, Blanca Cousiño, Zunilda Sanchez, Laura X Franco, Eva M Nara, Lilian Chena, Magaly Martínez, María E Galeano, Lucio Benitez
BACKGROUND: Since the early 1990s, programs to control Chagas disease in South America have focused on eradicating domiciliary Triatoma infestans, the main vector. Seroprevalence studies of the chagasic infection are included as part of the vector control programs; they are essential to assess the impact of vector control measures and to monitor the prevention of vector transmission. OBJECTIVE: To assess the interruption of domiciliary vector transmission of Chagas disease by T...
May 2017: Memórias do Instituto Oswaldo Cruz
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