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https://www.readbyqxmd.com/read/28801691/a-new-data-processing-routine-facilitating-the-identification-of-surface-adhered-proteins-from-bacterial-conditioning-films-via-qcm-d-maldi-tof-ms
#1
Siegfried Hohmann, Anke Neidig, Boris Kühl, Frank Kirschhöfer, Jörg Overhage, Gerald Brenner-Weiß
Conditioning films are an important factor in the initiation and development of microbial biofilms, which are the leading cause of chronic infections associated with medical devices. Here, we analyzed the protein content of conditioning films formed after exposure to supernatants of cultures of the human pathogen Pseudomonas aeruginosa PAO1. Adhesion of substances from the supernatant was monitored using quartz crystal microbalance with dissipation monitoring (QCM-D) sensor chips modified with the commonly used implant material titanium dioxide (TiO2)...
August 11, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28798972/diagnostic-precision-of-triage-algorithms-for-mass-casualty-incidents-english-version
#2
A R Heller, N Salvador, M Frank, J Schiffner, R Kipke, C Kleber
BACKGROUND: Regarding survival and quality of life recent mass casualty incidents again emphasize the importance of early identification of the correct degree of injury/illness to enable prioritization of treatment amongst patients and their transportation to an appropriate hospital. The present study investigated existing triage algorithms in terms of sensitivity (SE) and specificity (SP) as well as its process duration in a relevant emergency patient cohort. METHODS: In this study 500 consecutive air rescue missions were evaluated by means of standardized patient records...
August 10, 2017: Der Anaesthesist
https://www.readbyqxmd.com/read/28776774/identification-of-some-bioactive-metabolites-in-a-fractionated-methanol-extract-from-ipomoea-aquatica-aerial-parts-through-tlc-hplc-uplc-esi-qtof-ms-and-lc-spe-nmr-fingerprints-analyses
#3
Mahmoud Hefny Gad, Emmy Tuenter, Nagwa El-Sawi, Sabry Younes, El-Mewafy El-Ghadban, Kristiaan Demeyer, Luc Pieters, Yvan Vander Heyden, Debby Mangelings
INTRODUCTION: The plant species Ipomoea aquatica contains various bioactive constituents, e.g. phenols and flavonoids, which have several medical uses. All previous studies were executed in Asia; however, no reports are available from Africa, and the secondary metabolites of this plant species from Africa are still unknown. OBJECTIVE: The present study aims finding suitable conditions to identify the bioactive compounds from different fractions. METHODOLOGY: Chromatographic fingerprint profiles of different fractions were developed using high-performance liquid chromatography (HPLC) and then these conditions were transferred to thin-layer chromatography (TLC)...
August 4, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28744481/visualizing-antimicrobials-in-bacterial-biofilms-three-dimensional-biochemical-imaging-using-tof-sims
#4
Sarah K Davies, Sarah Fearn, Luke P Allsopp, Freya Harrison, Ecaterina Ware, Stephen P Diggle, Alain Filloux, David S McPhail, Jacob G Bundy
Bacterial biofilms are groups of bacteria that exist within a self-produced extracellular matrix, adhering to each other and usually to a surface. They grow on medical equipment and inserts such as catheters and are responsible for many persistent infections throughout the body, as they can have high resistance to many antimicrobials. Pseudomonas aeruginosa is an opportunistic pathogen that can cause both acute and chronic infections and is used as a model for research into biofilms. Direct biochemical methods of imaging of molecules in bacterial biofilms are of high value in gaining a better understanding of the fundamental biology of biofilms and biochemical gradients within them...
July 2017: MSphere
https://www.readbyqxmd.com/read/28744243/the-odyssey-of-dental-anxiety-from-prehistory-to-the-present-a-narrative-review
#5
REVIEW
Enrico Facco, Gastone Zanette
Dental anxiety (DA) can be considered as a universal phenomenon with a high prevalence worldwide; DA and pain are also the main causes for medical emergencies in the dental office, so their prevention is an essential part of patient safety and overall quality of care. Being DA and its consequences closely related to the fight-or-flight reaction, it seems reasonable to argue that the odyssey of DA began way back in the distant past, and has since probably evolved in parallel with the development of fight-or-flight reactions, implicit memory and knowledge, and ultimately consciousness...
2017: Frontiers in Psychology
https://www.readbyqxmd.com/read/28734043/enabling-reduction-of-low-grade-inflammation-in-seniors-pilot-study-concept-rationale-and-design
#6
Todd M Manini, Stephen D Anton, Daniel P Beavers, Jane A Cauley, Mark A Espeland, Roger A Fielding, Stephen B Kritchevsky, Christiaan Leeuwenburgh, Kristina H Lewis, Christine Liu, Mary M McDermott, Michael E Miller, Russell P Tracy, Jeremy D Walston, Barbara Radziszewska, Jane Lu, Cindy Stowe, Samuel Wu, Anne B Newman, Walter T Ambrosius, Marco Pahor
OBJECTIVES: To test two interventions to reduce interleukin (IL)-6 levels, an indicator of low-grade chronic inflammation and an independent risk factor for impaired mobility and slow walking speed in older adults. DESIGN: The ENabling Reduction of low-Grade Inflammation in SEniors (ENRGISE) Pilot Study was a multicenter, double-blind, placebo-controlled randomized pilot trial of two interventions to reduce IL-6 levels. SETTING: Five university-based research centers...
July 22, 2017: Journal of the American Geriatrics Society
https://www.readbyqxmd.com/read/28722283/comparative-analysis-of-storage-conditions-and-homogenization-methods-for-tick-and-flea-species-for-identification-by-maldi-tof-ms
#7
A Nebbak, B El Hamzaoui, J-M Berenger, I Bitam, D Raoult, L Almeras, P Parola
Ticks and fleas are vectors for numerous human and animal pathogens. Controlling them, which is important in combating such diseases, requires accurate identification, to distinguish between vector and non-vector species. Recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was applied to the rapid identification of arthropods. The growth of this promising tool, however, requires guidelines to be established. To this end, standardization protocols were applied to species of Rhipicephalus sanguineus (Ixodida: Ixodidae) Latreille and Ctenocephalides felis felis (Siphonaptera: Pulicidae) Bouché, including the automation of sample homogenization using two homogenizer devices, and varied sample preservation modes for a period of 1-6 months...
July 19, 2017: Medical and Veterinary Entomology
https://www.readbyqxmd.com/read/28715745/a-practical-protocol-for-comprehensive-evaluation-of-sulfur-fumigation-of-gastrodia-rhizoma-using-metabolome-and-health-risk-assessment-analysis
#8
Chuanzhi Kang, Chang-Jiang-Sheng Lai, Dan Zhao, Tao Zhou, Da-Hui Liu, Chaogeng Lv, Sheng Wang, Liping Kang, Jian Yang, Zhi-Lai Zhan, Lu-Qi Huang, Lanping Guo
Gastrodia Rhizoma is one of the most heavily sulfur-fumigated edible and medical herbs in the marketplace. We developed a practical protocol using an ultra-performance liquid chromatography coupled with quadrupole time-of-flight-MS(E) (UPLC/QTOF-MS(E))-based metabolome and health risk assessment model to identify characteristic sulfur-fumigated markers, dissect chemical transformation mechanisms, and control the quality of sulfur-fumigated Gastrodia Rhizoma. Two sulfur-containing p-hydroxybenzyl products, one sulfur-containing disaccharide, one glycolipid, and two phospholipids were selected and identified as markers based on multivariate statistical analysis...
July 4, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28703095/identification-of-microorganisms-by-modern-analytical-techniques
#9
Boguław Buszewski, Agnieszka Rogowska, Paweł Pomastowski, Michał Złoch, Viorica Railean-Plugaru
Rapid detection and identification of microorganisms is a challenging and important aspect in a wide range offields, from medical to industrial, affecting human lives. Unfortunately, classical methods of microorganism identification are based on time-consuming and labor-intensive approaches. Screening techniques require the rapid and cheap grouping of bacterial isolates; however, modern bioanalytics demand comprehensive bacterial studies at a molecular level. Modern approaches for the rapid identification of bacteria use molecular techniques, such as 16S ribosomal RNA gene sequencing based on polymerase chain reaction or electromigration, especially capillary zone electrophoresis and capillary isoelectric focusing...
July 13, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28687801/altered-kyn-trp-gln-glu-and-met-methionine-sulfoxide-ratios-in-the-blood-plasma-of-medication-free-patients-with-major-depressive-disorder
#10
Hidehiro Umehara, Shusuke Numata, Shin-Ya Watanabe, Yutaka Hatakeyama, Makoto Kinoshita, Yukiko Tomioka, Kiyoshi Nakahara, Takeshi Nikawa, Tetsuro Ohmori
Capillary electrophoresis-time-of-flight mass spectrometry (CE-TOFMS) is a comprehensive, quantitative, and high throughput tool used to analyze metabolite profiles. In the present study, we used CE-TOFMS to profile metabolites found in the blood plasma of 33 medication-free patients with major depressive disorder (MDD) and 33 non-psychiatric control subjects. We then investigated changes which occurred in the metabolite levels during an 8-week treatment period. The medication-free MDD patients and control subjects showed significant differences in their mean levels of 33 metabolites, including kynurenine (KYN), glutamate (Glu), glutamine (Gln), methionine sulfoxide, and methionine (Met)...
July 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28671519/acinetobacter-colistiniresistens-sp-nov-formerly-genomic-species-13-sensu-bouvet-and-jeanjean-and-genomic-species-14-sensu-tjernberg-and-ursing-isolated-from-human-infections-and-characterized-by-intrinsic-resistance-to-polymyxins
#11
Alexandr Nemec, Lenka Radolfova-Krizova, Martina Maixnerova, Ondrej Sedo
Strains of the genusAcinetobacter, classified as genomic species 13BJ/14TU have been previously associated with human infections and resistance to colistin. To clarify the taxonomy of this provisional group, we investigated 24 strains that have been isolated from humans since the 1960s in 10 countries. The genus-wide analysis of the rpoB and gyrB sequences of all strains and whole-genome sequences of strains representing different rpoB/gyrB genotypes showed that the 24 strains formed a distinct monophyletic group within the so-called haemolytic clade of the genus Acinetobacter...
July 3, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28664820/ten-thousand-kilometre-transfer-of-cardiogenic-shock-patients-on-venoarterial-extracorporeal-membrane-oxygenation-for-emergency-heart-transplantation-cooperation-between-reunion-island-and-metropolitan-france
#12
Clément Charon, Jérôme Allyn, Bruno Bouchet, Fréderic Nativel, Eric Braunberger, Caroline Brulliard, Olivier Martinet, Nicolas Allou
BACKGROUND: There is no heart transplantation centre on the French overseas territory of Reunion Island (distance of 10,000 km). The aim of this study was to describe the characteristics of cardiogenic shock adult patients on venoarterial extracorporeal membrane oxygenation (VA-ECMO) who were transferred from Reunion Island to mainland France for emergency heart transplantation. METHODS: This retrospective observational study was conducted between 2005 and 2015...
June 1, 2017: European Heart Journal. Acute Cardiovascular Care
https://www.readbyqxmd.com/read/28662061/ultra-high-field-upper-extremity-peripheral-nerve-and-non-contrast-enhanced-vascular-imaging
#13
Shailesh B Raval, Cynthia A Britton, Tiejun Zhao, Narayanan Krishnamurthy, Tales Santini, Vijay S Gorantla, Tamer S Ibrahim
OBJECTIVE: The purpose of this study was to explore the efficacy of Ultra-high field [UHF] 7 Tesla [T] MRI as compared to 3T MRI in non-contrast enhanced [nCE] imaging of structural anatomy in the elbow, forearm, and hand [upper extremity]. MATERIALS AND METHOD: A wide range of sequences including T1 weighted [T1] volumetric interpolate breath-hold exam [VIBE], T2 weighted [T2] double-echo steady state [DESS], susceptibility weighted imaging [SWI], time-of-flight [TOF], diffusion tensor imaging [DTI], and diffusion spectrum imaging [DSI] were optimized and incorporated with a radiofrequency [RF] coil system composed of a transverse electromagnetic [TEM] transmit coil combined with an 8-channel receive-only array for 7T upper extremity [UE] imaging...
2017: PloS One
https://www.readbyqxmd.com/read/28630743/comparison-of-five-video-assisted-intubation-devices-by-novice-and-expert-laryngoscopists-for-use-in-the-aeromedical-evacuation-environment
#14
Matthew C Wallace, SSgt Tyler Britton, Robbie Meek, Sharon Walsh-Hart, Col Todd E Carter, Steven J Lisco
BACKGROUND: The critically ill or injured patient undergoing military medical evacuation may require emergent intubation. Intubation may be life-saving, but it carries risks. The novice or infrequent laryngoscopist has a distinct disadvantage because experience is critical for the rapid and safe establishment of a secured airway. This challenge is compounded by the austere environment of the back of an aircraft under blackout conditions. This study determined which of five different video-assisted intubation devices (VAIDs) was best suited for in-flight use by U...
2017: Military Medical Research
https://www.readbyqxmd.com/read/28628474/prediction-of-human-iron-bioavailability-using-rapid-c-elisas-for-human-plasma-hepcidin
#15
Nicole U Stoffel, Christophe Zeder, Eloïse Fort, Dorine W Swinkels, Michael B Zimmermann, Diego Moretti
BACKGROUND: Hepcidin is the central systemic regulator of iron metabolism, but its quantification in biological fluids is challenging. Rapid, accurate and user-friendly methods are needed. Our aim was to assess the ability of hepcidin as measured by three different c-ELISA assays to predict iron bioavailability in humans. METHODS: The three assays used were commercially available DRG and Peninsula assays and the c-ELISA method performed at Radboud University Medical Centre, Nijmegen, The Netherlands (Hepcidinanalysis...
July 26, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28628427/is-there-a-doctor-on-board-in-flight-medical-emergencies
#16
REVIEW
Jose V Nable, Christina L Tupe, Bruce D Gehle, William J Brady
Although not legally required to render assistance in the event of a medical emergency aboard an airplane, physicians have an ethical obligation to do so and should be prepared.
June 2017: Cleveland Clinic Journal of Medicine
https://www.readbyqxmd.com/read/28627917/functional-intimacy-needing-but-not-wanting-the-touch-of-a-stranger
#17
Juliana Schroeder, Ayelet Fishbach, Chelsea Schein, Kurt Gray
Intimacy is often motivated by love, but sometimes it is merely functional. For example, disrobing and being touched at an airport security check serves the goal of catching a flight, not building a relationship. We propose that this functional intimacy induces discomfort, making people prefer greater social distance from their interaction partner. Supporting this prediction, participants who considered (Experiments 1 and 2) or experienced (Experiment 3) more physically intimate medical procedures preferred a health provider who is less social...
June 19, 2017: Journal of Personality and Social Psychology
https://www.readbyqxmd.com/read/28608586/metabolism-of-novel-opioid-agonists-u-47700-and-u-49900-using-human-liver-microsomes-with-confirmation-in-authentic-urine-specimens-from-drug-users
#18
Alex J Krotulski, Amanda L A Mohr, Donna M Papsun, Barry K Logan
Recently, the number of adverse events, including death, involving novel opioids has continued to increase, providing additional and sustained challenges for forensic and medical communities. Identification of emerging novel opioids can be challenging, compounded by detection windows and unknown metabolic profiles. In this study, human liver microsomes were used for the generation of in vitro metabolic profiles of U-47700 and U-49900. Generated metabolites were analyzed via a SCIEX TripleTOF® 5600+ quadrupole time-of-flight mass spectrometer and resulting data files were processing using MetabolitePilot™...
June 13, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/28606186/comparison-of-chemical-profiles-between-the-root-and-aerial-parts-from-three-bupleurum-species-based-on-a-uhplc-qtof-ms-metabolomics-approach
#19
Lin Zhu, Zhi-Tao Liang, Tao Yi, Yue Ma, Zhong-Zhen Zhao, Bao-Lin Guo, Jian-Ye Zhang, Hu-Biao Chen
BACKGROUND: Bupleuri Radix (Chaihu) represents one of the most successful and widely used herbal medicines in Asia for the treatment of many diseases such as inflammatory disorders and infectious diseases over the past 2000 years. In the Chinese Pharmacopoeia, Chaihu is recorded as the dried roots of Bupleurum chinense DC. and B. scorzonerifolium Willd. (Umbelliferae). However, the widespread demand for the herb has tended to far outstrip the supply. Whether the aerial parts, which account for 70 ~ 85% of the dry weights of Bupleurum species, could be used as an alternative for the root has become an important scientific issue for the sustainable utilization of Bupleurum species...
June 12, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28606064/bacteremia-due-to-gordonia-polyisoprenivorans-case-report-and-review-of-literature
#20
Xiurong Ding, Yanhua Yu, Ming Chen, Chen Wang, Yanfang Kang, Hongman Li, Jinli Lou
BACKGROUND: Gordonia polyisoprenivorans is a ubiquitous aerobic actinomycetes bacterium that rarely cause infections in humans. Here, we report a case of G. polyisoprenivorans catheter-related bacteremia in an AIDS patient. CASE PRESENTATION: A 37-year-old man with a past medical history of AIDS-related lymphoma suffered bacteremia caused by a Gram-positive corynebacterium. The strain was identified as a Gordonia species by matrix-assisted laser desorption ionization-time of flight mass spectrometry and confirmed to G...
June 12, 2017: BMC Infectious Diseases
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