keyword
https://read.qxmd.com/read/38530900/structural-and-mechanistic-basis-of-the-central-energy-converting-methyltransferase-complex-of-methanogenesis
#21
JOURNAL ARTICLE
Iram Aziz, Kanwal Kayastha, Susann Kaltwasser, Janet Vonck, Sonja Welsch, Bonnie J Murphy, Jörg Kahnt, Di Wu, Tristan Wagner, Seigo Shima, Ulrich Ermler
Methanogenic archaea inhabiting anaerobic environments play a crucial role in the global biogeochemical material cycle. The most universal electrogenic reaction of their methane-producing energy metabolism is catalyzed by N     5 -methyl-tetrahydromethanopterin: coenzyme M methyltransferase (MtrABCDEFGH), which couples the vectorial Na+ transport with a methyl transfer between the one-carbon carriers tetrahydromethanopterin and coenzyme M via a vitamin B12 derivative (cobamide) as prosthetic group...
April 2, 2024: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/38530522/performance-evaluation-of-anaerobic-aerobic-hybrid-baffled-reactor-coupled-with-an-anaerobic-filter-treating-landfill-leachate
#22
JOURNAL ARTICLE
Zabihollah Yousefi, Esmaeil Babanezhad, Yalda Hashempour, Reza Ali Mohammadpour, Fatemeh Mortezazadeh
The Anaerobic Baffled Reactor (ABR) is an effective solution for landfill leachate treatment using an anaerobic fermentation process, which helps to reduce operating costs and sludge volume. To better understand the biological, chemical, and physical processes involved, especially when combining the ABR with an aerobic component, the study aimed to investigate the performance of an Anaerobic-Aerobic Hybrid Baffled Reactor (AABR) that includes an Anaerobic Filter (AF) for treating landfill leachate. This research utilized two glass reactors...
March 26, 2024: Environmental Science and Pollution Research International
https://read.qxmd.com/read/38529905/-fusobacterium-nucleatum-subsp-polymorphum-recovered-from-malignant-and-potentially-malignant-oral-disease-exhibit-heterogeneity-in-adhesion-phenotypes-and-adhesin-gene-copy-number-shaped-by-inter-subspecies-horizontal-gene-transfer-and-recombination-derived
#23
JOURNAL ARTICLE
Claire Crowley, Ajith Selvaraj, Arvind Hariharan, Claire M Healy, Gary P Moran
Fusobacterium nucleatum is an anaerobic commensal of the oral cavity associated with periodontitis and extra-oral diseases, including colorectal cancer. Previous studies have shown an increased relative abundance of this bacterium associated with oral dysplasia or within oral tumours. Using direct culture, we found that 75 % of Fusobacterium species isolated from malignant or potentially malignant oral mucosa were F. nucleatum subsp. polymorphum . Whole genome sequencing and pangenome analysis with Panaroo was carried out on 76 F ...
March 2024: Microbial Genomics
https://read.qxmd.com/read/38529709/acute-alcohol-ingestion-decreases-the-work-done-above-the-end-test-power-during-a-3-min-all-out-cycling-exercise
#24
JOURNAL ARTICLE
Danilo França Conceição Dos Santos, Samuel Penna Wanner, Rodolfo Ferreira de Paula, Gustavo Oliveira Zanetti, Donizete Cícero Xavier de Oliveira, Fabio Lera Orsatti, Francisco Teixeira-Coelho
INTRODUCTION: Alcohol ingestion influences metabolism during a subsequent exercise session, as evidenced by increased blood lactate concentration during fixed-intensity exercise. Therefore, augmented blood concentrations of alcohol may interfere with the anaerobic metabolism during high-intensity, short-duration exercise bout, thereby leading to impaired athletic performance. OBJECTIVE: This study investigated whether the acute ingestion of alcohol as ethanol modulates performance parameters derived from the power-duration relationship in a 3-min all-out cycling test that allows for identifying the power output related to heavy and severe exercise intensities...
March 16, 2024: Alcohol and Alcoholism
https://read.qxmd.com/read/38528188/pseudodesulfovibrio-pelocollis-sp-nov-a-sulfate-reducing-bacterium-isolated-from-a-terrestrial-mud-volcano
#25
JOURNAL ARTICLE
Galina Slobodkina, Alexander Merkel, Andrei Novikov, Alexander Slobodkin
Terrestrial mud volcanoes (TMVs), surface expressions of a deep-subterranean sedimentary volcanism, are widespread throughout the world. The methane and sulfur cycles are recognized as the most important biogeochemical cycles in these environments. Only few anaerobic bacterial strains were recovered from TMVs. We have isolated a novel sulfate-reducing bacterium (strain SB368T ) from TMV located at Taman Peninsula, Russia. Optimum growth of strain SB368T was observed at 30 °C, pH 8.0 and 1% NaCl. Strain SB368T utilized lactate, pyruvate and fumarate in the presence of sulfate, sulfite or thiosulfate...
March 25, 2024: Current Microbiology
https://read.qxmd.com/read/38528061/menopausal-status-induces-vaginal-dysbiosis-in-women-with-human-papillomavirus-infection
#26
JOURNAL ARTICLE
Kyeong A So, Soo Young Hur, Chi Heum Cho, Jae Kwan Lee, Seok Ju Seong, Dae Hoon Jeong, Moran Ki, Tae Jin Kim
In this study, we examined the difference in the vaginal microbiota of women infected with human papillomavirus (HPV), according to menopausal status. A total of 75 cervicovaginal swab samples from 38 pre- and 37 postmenopausal women with HPV infection were obtained from the Korean HPV cohort. Vaginal microbiota analysis, including microbial diversity and specific bacterial abundances, was performed using 16S rRNA gene sequencing. The mean age of the pre- and postmenopausal women were 29.5 and 55.8 years, respectively (p < 0...
March 26, 2024: Scientific Reports
https://read.qxmd.com/read/38527882/biodegradability-of-bioplastics-in-different-aquatic-environments-a-systematic-review
#27
REVIEW
Maria Cristina Lavagnolo, Valentina Poli, Anna Maria Zampini, Valentina Grossule
Bioplastics were first introduced as environmentally friendly materials, with properties similar to those of conventional plastics. A bioplastic is defined as biodegradable if it can be decomposed into carbon dioxide under aerobic degradation, or methane and CO2 under anaerobic conditions, inorganic compounds, and new cellular biomass, by the action of naturally occurring microorganisms. This definition however does not provide any information on the environmental conditions, timescale and extent at which decomposition processes should occur...
August 2024: Journal of Environmental Sciences (China)
https://read.qxmd.com/read/38527737/dietary-methionine-enhances-portal-appearance-of-guanidinoacetate-and-synthesis-of-creatine-in-yucatan-miniature-piglets
#28
JOURNAL ARTICLE
Mahesha U Asiriwardhana, O Chandani Dinesh, Janet A Brunton, Robert F Bertolo
BACKGROUND: Creatine plays a significant role in energy metabolism and positively impacts anaerobic energy capacity, muscle mass, and physical performance. Endogenous creatine synthesis requires guanidinoacetic acid (GAA) and methionine. GAA can be an alternative to creatine supplements and has been tested as a beneficial feed additive in the animal industry. When pigs are fed GAA with excess methionine, creatine is synthesized without feedback regulation. In contrast, when dietary methionine is limiting, creatine synthesis is limited, yet GAA does not accumulate in plasma, urine, or liver...
March 23, 2024: Journal of Nutrition
https://read.qxmd.com/read/38527723/anaerobic-membrane-bioreactor-coupled-with-polyaluminum-chloride-for-high-strength-phenolic-wastewater-treatment-robust-performance-and-potential-mechanisms
#29
JOURNAL ARTICLE
Chao Pang, Shun Wang, Chunhua He, Mengqi Zheng, Wei Wang
Anaerobic digestion of phenolic wastewater by anaerobic membrane bioreactor (AnMBR) has revealed increasing attractiveness, but the application of AnMBRs for treating high-strength phenolic wastewater faces challenges related to elevated phenol stress and membrane fouling. In this study, the coupling of AnMBR and polyaluminum chloride (PAC) was developed for efficient treatment of high-strength phenolic wastewater. The system achieved robust removal efficiencies of phenol (99%) and quinoline (98%) at a gradual increase of phenol concentration from 1000 to 5000 mg/L and a constant quinoline concentration of 100 mg/L...
March 23, 2024: Environmental Research
https://read.qxmd.com/read/38527650/clostridioides-difficile-infection-promotes-gastrointestinal-dysfunction-in-human-and-mice-post-acute-phase-of-the-disease
#30
JOURNAL ARTICLE
Deiziane V S Costa, Nathalie V Pham, Andrea V Loureiro, Suemin E Yang, Brian W Behm, Cirle A Warren
OBJECTIVES: In the US, Clostridioides difficile (C. difficile) infection (CDI) is the 8th leading cause for hospital readmission and 7th for mortality among all gastrointestinal (GI) disorders. Here, we investigated GI dysfunction post-CDI in human and mice post-acute infection. MATERIALS AND METHODS: From March 2020 to July 2021, we reviewed the clinical records of 67 patients referred to the UVA Complicated C. difficile clinic for fecal microbiota transplantation (FMT) eligibility...
March 23, 2024: Anaerobe
https://read.qxmd.com/read/38526862/effect-of-regular-physical-activity-and-lifestyle-changes-on-insulin-resistance-in-patients-after-kidney-transplantation
#31
JOURNAL ARTICLE
Karol Granak, Matej Vnucak Monika Beliancinova, Ivana Dedinska
Insulin resistance (IR) is the most significant risk factor for post-transplant diabetes mellitus (PTDM). This study aimed to determine the effects of regular physical activity on IR and PTDM in patients after kidney transplantation (KT). The study group (n = 22) participated in aerobic or combined sports (aerobic and anaerobic). Monitoring was provided by a sports tracker (Xiaomi Mi Band 4, compatible with the Mi Fit mobile application). Waist circumference was significantly lower (p = 0.0437, p = 0...
2024: Bratislavské Lekárske Listy
https://read.qxmd.com/read/38526092/circular-metagenome-assembled-genome-of-candidatus-patescibacteria-recovered-from-anaerobic-digestion-sludge
#32
JOURNAL ARTICLE
Riku Sakurai, Yasuhiro Fukuda, Chika Tada
A single-contig, circular metagenome-assembled genome (cMAG) of Candidatus ( Ca. ) Patescibacteria was reconstructed from a mesophilic full-scale food waste treatment plant in Japan. The genome is of small size and lacks fundamental biosynthetic pathways. Taxonomic analysis using the Genome Taxonomy Database revealed that this cMAG belonged to the genus JAEZRQ01 ( Ca . Parcubacteria).
March 25, 2024: Microbiology Resource Announcements
https://read.qxmd.com/read/38525664/cadaver-preservative-properties-of-a-solution-composed-of-honey-ethyl-alcohol-liquid-paraffin-distilled-water-and-citric-acid-experiments-on-rabbit-cadavers
#33
JOURNAL ARTICLE
Yasin Demiraslan, İftar Gürbüz, Mehmet Özbek, Özlem Şahan Yapicier, Harun Karaca, Özcan Özgel, Hakan Öner
The objective of this study is to assess the efficacy of a solution including honey, ethyl alcohol, liquid paraffin, distilled water and citric acid (HEFS) as a preservative for rabbit cadavers, serving as a potential substitute for formaldehyde. The cadavers underwent preservation using three distinct solutions: 10% formalin, 35% alcohol and HEFS. The cadavers were subjected to a total of four sampling events, occurring at 4-month intervals, in order to collect specimens for microanatomical, histological, microbiological, mycological, colourimetric, texture and odour analysis...
May 2024: Anatomia, Histologia, Embryologia
https://read.qxmd.com/read/38525221/analysis-of-the-season-dependent-component-in-the-evaluation-of-morphological-and-biochemical-blood-parameters-in-shetland-ponies-of-both-sexes-during-exercise
#34
JOURNAL ARTICLE
Halina Tkaczenko, Oleksandr Lukash, Natalia Kurhaluk
INTRODUCTION: Determination of morphological and biochemical blood indices facilitates assessment of the health and welfare of horses, their nutrient demand, the effects of training already undertaken, and the horses' suitability for exercise. Identification of the season-dependent components and the effects of sex and exercise on changes in frequently referenced haematological and biochemical parameters was the main goal of the current study. MATERIAL AND METHODS: The blood morphology of 21 healthy adult Shetland ponies (11 mares and 10 stallions) aged 6...
March 2024: Journal of Veterinary Research
https://read.qxmd.com/read/38524237/detection-of-typical-indigenous-gut-bacteria-related-to-kanpyo-lagenaria-siceraria-var-hispida-powder-in-murine-caecum-and-human-faecal-cultures
#35
JOURNAL ARTICLE
Mahiro Yamamoto, Hikaru Ogura, Takashi Kuda, Yumeng Xia, Ayaka Nakamura, Hajime Takahashi, Junji Inoue, Shu Takayanagi
Kanpyo (KP) is an edible dried product produced by peeling the fruit of the gourd Lagenaria siceraria var . hispida ; it is used in the traditional Japanese cuisine. The health functionality of KP due to its rich dietary fibre is expected to include a possible combined effect of KP-responsive indigenous gut bacteria (KP-RIB). However, its effect on the gut microbiota is unclear. To determine the effects of the KP on the gut microbiota and their host, Institute of Cancer Research mice were fed a high-sucrose diet containing no fibre (NF) or 5% (w/w) KP for 14 days, and their caecal microbiota was analysed by 16S rRNA (V4) amplicon sequencing...
April 2024: 3 Biotech
https://read.qxmd.com/read/38523602/myd88-exacerbates-inflammation-induced-bone-loss-by-modulating-dynamic-equilibrium-between-th17-treg-cells-and-subgingival-microbiota-dysbiosis
#36
JOURNAL ARTICLE
Po-Yan Hsiao, Ren-Yeong Huang, Lin-Wei Huang, Ching-Liang Chu, Thomas Van Dyke, Lian-Ping Mau, Chia-Dan Cheng, Cheng-En Sung, Pei-Wei Weng, Yu-Chiao Wu, Yi-Shing Shieh, Wan-Chien Cheng
BACKGROUND: This study aimed to investigate the contribution of myeloid differentiation primary-response gene 88 (MyD88) on the differentiation of T helper type 17 (Th17) and regulatory T (Treg) cells and the emerging subgingival microbiota dysbiosis in Porphyromonas gingivalis-induced experimental periodontitis. METHODS: Alveolar bone loss, infiltrated inflammatory cells, immunostained cells for tartrate-resistant acid phosphatase (TRAP), the receptor activator of nuclear factor-kB ligand (RANKL), and osteoprotegerin (OPG) were quantified by microcomputerized tomography and histological staining between age- and sex-matched homozygous littermates (wild-type [WT, Myd88+/+ ] and Myd88-/- on C57BL/6 background)...
March 25, 2024: Journal of Periodontology
https://read.qxmd.com/read/38523189/antibacterial-and-hemocompatibility-potentials-of-nano-gold-cored-alginate-preparation-against-anaerobic-bacteria-from-acne-vulgaris
#37
JOURNAL ARTICLE
Hanan A Abbas, Ali A Taha, Ghassan M Sulaiman, Amer Al Ali, Humood Al Shmrany, Haralambos Stamatis, Hamdoon A Mohammed, Riaz A Khan
Acne is a prevalent dermatological disease, with high global incidence, and is a health menace. The current study aimed to isolate and characterize the anaerobic bacteria responsible for the condition. Causes of a total of 70 acne-based bacterium isolates obtained from patients of mild, moderate, and severe acne, 24 were Clostridium innocuum, 21 were Lactobacillus plantarum, 13 were Anaerococcus prevotii, and 12 were Peptoniphilus asaccharolyticus. Nearly 69% of males were suffering, while the rest were females at 31%...
March 24, 2024: Scientific Reports
https://read.qxmd.com/read/38523174/modeling-key-intermediates-during-anaerobic-digestion-of-lipid-rich-kitchen-waste-with-an-extended-adm1
#38
JOURNAL ARTICLE
Simon Weber
Quantitative dynamics of the key intermediates, gases and carbohydrates during anaerobic digestion of different lipid rich kitchen waste and lipid rich model kitchen waste were modeled. Six batch reactors loaded with 25 g <mml:math xmlns:mml="https://www.w3.org/1998/Math/MathML"><mml:msub><mml:mrow/> <mml:mtext>VS</mml:mtext></mml:msub> </mml:math>  l <mml:math xmlns:mml="https://www.w3.org/1998/Math/MathML"><mml:msup><mml:mrow/> <mml:mrow><mml:mo>-</mml:mo> <mml:mn>1</mml:mn></mml:mrow> </mml:msup> </mml:math> ( <mml:math xmlns:mml="https://www...
March 25, 2024: Biodegradation
https://read.qxmd.com/read/38522745/effect-of-bacteria-algae-ratio-on-treatment-of-anaerobic-digested-wastewater-by-symbiotic-coupling-of-bacteria-and-algae-under-the-background-of-carbon-neutralization
#39
JOURNAL ARTICLE
Zhipeng Liu, Lei Feng, Chenxi Liu
Environmental pollution is a growing concern, particularly the impact of sewage treatment gas on the atmosphere's greenhouse effect. Efficient sewage resource recycling is crucial to achieving carbon neutrality. The bacteria-algae symbiotic sewage treatment system combines wastewater treatment, carbon dioxide fixation, and biomass energy recovery to achieve the goal of carbon neutrality, environmental protection, and the transformation of high-value added products. This paper presents the construction of a sequencing batch photobiological reaction system that utilizes a microbial-algae symbiotic relationship...
March 22, 2024: Environmental Research
https://read.qxmd.com/read/38522678/dual-disintegration-of-microalgae-biomass-for-cost-effective-biomethane-production-energy-and-cost-assessment
#40
JOURNAL ARTICLE
Yukesh Kannah Ravi, S Kavitha, Siham Y Al-Qaradawi, J Rajesh Banu
The present study aims to enhance the biomethane production potential of microalgae via a dual disintegration process. During this process, the microalgae biomass was firstly subjected to cell wall weakening by thermochemical disintegration (TC) (50 to 80 °C), pH adjustment with alkali, NaOH (6 to 10) and time (0 to 10 min) and, secondly, by bacterial disintegration (BD). TC-BD disintegration was comparatively higher (33 %) than BD (24 %), TC (8.5 %), and control (7 %)...
March 22, 2024: Bioresource Technology
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