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https://www.readbyqxmd.com/read/28651321/methane-production-and-characteristics-of-the-microbial-community-in-a-two-stage-fixed-bed-anaerobic-reactor-using-molasses
#1
Xingyao Meng, Xufeng Yuan, Jiwei Ren, Xiaofen Wang, Wanbin Zhu, Zongjun Cui
Molasses is a typical feedstock for fermentation, but the effluent is hard to treat. In this study, molasses containing a high concentration of organic matter was treated by a two-stage Fix-bed reactor system with an increased organic loading rate (OLR). The results indicated at high molasses loading rate, the two-stage system was more efficient (i.e. organic matter removal, the COD of effluent and biogas production) than the single-stage system. The relative abundance of Anaerolineaceae and W5_norank was higher in the first stage (R1), where these organisms digest carbohydrates, while the second stage (R2) had higher relative abundance of Synergistaceae and SB-1_norank, which digest VFAs and decomposition-resistant compounds to produce compounds used by hydrogen methanogens...
May 31, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28651317/effects-of-multi-environmental-factors-on-physiological-and-biochemical-responses-of-large-yellow-croaker-larimichthys-crocea
#2
Qian-Feng Wang, Wei-Liang Shen, Cheng Liu, Dan-Li Mu, Xiong-Fei Wu, Nian-Gang Guo, Jun-Quan Zhu
Land-based recirculating aquaculture systems (RAS) and cage culture are important methods of Larimichthys crocea production. The effects of environmental factors on physiological and biochemical aspects of L. crocea require clarification. Temperature and salinity are controlled in RAS and directly affect L. crocea growth and survival. To explore optimal parameters, the oxygen consumption rate (RO), ammonium excretion rate (RN), and O/N ratio at different temperatures (8, 14, 20, 26, and 32 °C) and salinities (5, 15, 25, and 35‰) were determined...
June 13, 2017: Chemosphere
https://www.readbyqxmd.com/read/28651243/structure-of-the-lps-o-chain-from-fusobacterium-nucleatum-strain-12230
#3
Evgeny Vinogradov, Frank St Michael, Andrew D Cox
Fusobacterium nucleatum is an anaerobic bacterium found in the human mouth where it causes periodontitis. It was also found in colorectal cancer tissues and is linked with pregnancy complications, including pre-term and still births. Cell surface structures of the bacterium could be implicated in pathogenesis. Here we report the following structure of the lipopolysaccharide O-chain of F. nucleatum strain 12230: -6-α-d-Glc-4-β-d-GlcNHBu3NHBuA-3-β-d-QuiNAc4NABu- where ABu and HBu indicate (R)-3-aminobutanoyl and (R)-3-hydroxybutanoyl, respectively; all monosaccharides are in the pyranose form...
June 16, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/28651138/effect-of-the-high-cross-flow-velocity-on-performance-of-a-pilot-scale-anaerobic-membrane-bioreactor-for-treating-antibiotic-solvent-wastewater
#4
Dongxue Hu, Tingting Xiao, Zhaobo Chen, Haixu Wang, Jiao Xu, Xue Li, Haiyan Su, Ying Zhang
The effect of high cross flow velocity (CFV) on the operational efficiency of a pilot-scale anaerobic membrane bioreactor (AnMBR) treating antibiotic solvent effluents was explored. The average of total Chemical Oxygen Demand (COD) and tetrahydrofuran (THF) removal efficiencies during four Runs were 96.5% and 98.7%. Meanwhile, biological removal contributions were 74.3% and 78%, the rest part was attributed by the physical removal process of the membrane block, VFA (Volatile Fatty Acids) and alpha value (VFA/alkalinity) increased with the increase of CFV, resulted alkalinity decreased...
June 9, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28651133/material-flow-and-sustainability-analyses-of-biorefining-of-municipal-solid-waste
#5
Jhuma Sadhukhan, Elias Martinez-Hernandez
This paper presents material flow and sustainability analyses of novel mechanical biological chemical treatment system for complete valorization of municipal solid waste (MSW). It integrates material recovery facility (MRF); pulping, chemical conversion; effluent treatment plant (ETP), anaerobic digestion (AD); and combined heat and power (CHP) systems producing end products: recyclables (24.9% by mass of MSW), metals (2.7%), fibre (1.5%); levulinic acid (7.4%); recyclable water (14.7%), fertiliser (8.3%); and electricity (0...
June 17, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28651084/treatment-of-food-waste-recycling-wastewater-using-anaerobic-ceramic-membrane-bioreactor-for-biogas-production-in-mainstream-treatment-process-of-domestic-wastewater
#6
Yeongmi Jeong, Slawomir W Hermanowicz, Chanhyuk Park
A bench-scale anaerobic membrane bioreactor (AnMBR) equipped with submerged flat-sheet ceramic membranes was operated at mesophilic conditions (30-35 °C) treating domestic wastewater (DWW) supplemented with food wasterecycling wastewater (FRW) to increase the organic loading rate (OLR) for better biogas production. Coupling ceramic membrane filtration with AnMBR treatment provides an alternative strategy for high organic wastewater treatment at short hydraulic retention times (HRTs) with the potential benefits of membrane fouling because they have a high hydrophilicity and more robust at extreme conditions...
June 19, 2017: Water Research
https://www.readbyqxmd.com/read/28650753/lower-extremity-infections-caused-by-serratia-marcescens-a-report-of-three-cases-and-a-literature-review
#7
Luis Marin, Raymond Rowan, Ana Mantilla, Bamidele Olupona, Ann MacIntyre
Serratia marcescens is a ubiquitous, facultatively anaerobic, gram-negative bacillus that has been cited to cause infection in immunocompromised populations. In the literature, S marcescens infections of the lower extremity have presented as granulomatous ulceration, abscess, bullous cellulitis, and necrotizing fasciitis. Herein we present a series of three cases of lower-extremity infections in which S marcescens was the sole or a contributing pathogen. We discuss the commonalities of these three cases as well as with those previously cited...
May 2017: Journal of the American Podiatric Medical Association
https://www.readbyqxmd.com/read/28650431/illuminating-vital-surface-molecules-of-symbionts-in-health-and-disease
#8
Jason E Hudak, David Alvarez, Ashwin Skelly, Ulrich H von Andrian, Dennis L Kasper
The immunomodulatory surface molecules of commensal and pathogenic bacteria are critical to microorganisms' survival and the host's response(1,2). Recent studies have highlighted the unique and important responses elicited by commensal-derived surface macromolecules(3-5). However, the technology available to track these molecules in host cells and tissues remains primitive. We report, here, an interdisciplinary approach that uses metabolic labelling combined with bioorthogonal click chemistry (that is, reactions performed in living organisms)(6) to specifically tag up to three prominent surface immunomodulatory macromolecules-peptidoglycan, lipopolysaccharide and capsular polysaccharide-either simultaneously or individually in live anaerobic commensal bacteria...
June 26, 2017: Nature Microbiology
https://www.readbyqxmd.com/read/28650071/in-vivo-synergistic-activity-of-a-cazyme-cassette-from-acidothermus-cellulolyticus-significantly-improves-the-cellulolytic-activity-of-the-c-bescii-exoproteome
#9
Sun-Ki Kim, Daehwan Chung, Michael E Himmel, Yannick J Bomble, Janet Westpheling
The use of microbial cells to convert plant biomass directly to fuels and chemicals is referred to as consolidated bioprocessing (CBP). Members of the bacterial genus, Caldicellulosiruptor (Gram-positive, anaerobic hyperthermophiles) are capable of deconstructing plant biomass without enzymatic or chemical pretreatment. This is accomplished by the production and secretion of free, multi-domain enzymes that outperform commercial enzyme cocktails on some substrates. Here, we show that the exoproteome of C. bescii may be enhanced by the heterologous expression of enzymes from Acidothermus cellulolyticus that act synergistically to improve sugar release from complex substrates; as well as improve cell growth...
June 26, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28649508/isolation-of-shewanella-putrefaciens-in-an-elderly-man-with-subacute-intestinal-obstruction-appendicitis
#10
Arif Maqsood Ali, Muhammad Noorulamin, Shazia Arif
Shewanella is Gram-negative motile bacillus, non fermentative and facultative anaerobe. Its natural habitat is all forms of water and soil, but has also been isolated from fish, dairy products, oils, and carcasses. Often found with microflora of the marine environment. Bacterial infections with Shewanella spp. are rare. The exposure to the marine environment, sea and diary food are considered as a risk factor for Shewanella spp. infection. Clinical infections seen are otitis, soft tissue infection, bacteremia, ear infection, eye infection, infective arthritis, osteomyelitis, infective endocarditis and peritonitis...
2017: IDCases
https://www.readbyqxmd.com/read/28649277/diversity-of-microbial-carbohydrate-active-enzymes-in-danish-anaerobic-digesters-fed-with-wastewater-treatment-sludge
#11
Casper Wilkens, Peter Kamp Busk, Bo Pilgaard, Wen-Jing Zhang, Kåre L Nielsen, Per Halkjær Nielsen, Lene Lange
BACKGROUND: Improved carbohydrate-active enzymes (CAZymes) are needed to fulfill the goal of producing food, feed, fuel, chemicals, and materials from biomass. Little is known about how the diverse microbial communities in anaerobic digesters (ADs) metabolize carbohydrates or which CAZymes that are present, making the ADs a unique niche to look for CAZymes that can potentiate the enzyme blends currently used in industry. RESULTS: Enzymatic assays showed that functional CAZymes were secreted into the AD environments in four full-scale mesophilic Danish ADs fed with primary and surplus sludge from municipal wastewater treatment plants...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28648748/optimized-anaerobic-aerobic-sequential-system-for-the-treatment-of-food-waste-and-wastewater
#12
S Ghanimeh, C Abou Khalil, C Bou Mosleh, C Habchi
Considering that modern wastewater and solid waste processing facilities seek efficient energy recovery methods, this study investigates anaerobic-aerobic sequential systems for combined treatment of raw wastewater with food waste. The optimum loading rate was found to be 1.6mgVSL(-1)d(-1) resulting in a stable operation of the anaerobic compartment. Yet, the increase in ammonia concentration resulted in gradual accumulation of VFA, until reaching a tipping point of 3000mgL(-1) beyond which an abrupt increase in VFA to above 6000mgL(-1) was observed, with acute stability loss and performance deterioration...
June 22, 2017: Waste Management
https://www.readbyqxmd.com/read/28648747/anaerobic-co-digestion-of-chicken-manure-and-microalgae-chlorella-sp-methane-potential-microbial-diversity-and-synergistic-impact-evaluation
#13
Ruirui Li, Na Duan, Yuanhui Zhang, Zhidan Liu, Baoming Li, Dongming Zhang, Taili Dong
Anaerobic digestion (AD) is a promising alternative for livestock manure management. This paper presents the experimental results obtained through a batch experiment by using chicken manure (CM) and microalgae Chlorella sp. as co-substrates. The effect of co-digestion was evaluated by varying CM to Chlorella sp. ratios (0:10, 2:8, 4:6, 6:4, 8:2, 10: 0 based on the volatile solids (VS)). The major objective of this study is to evaluate the feasibility and synergistic impact of co-digestion of CM and Chlorella sp...
June 22, 2017: Waste Management
https://www.readbyqxmd.com/read/28648746/anaerobic-digestion-of-solid-agroindustrial-waste-in-semi-continuous-mode-evaluation-of-mono-digestion-and-co-digestion-systems
#14
Frantseska-Maria Pellera, Evangelos Gidarakos
The present study aimed at investigating the anaerobic digestion of four agroindustrial waste, namely cotton gin waste, winery waste, olive pomace and juice industry waste, in semi-continuous mode, conducting mono-digestion and co-digestion assays, using an artificial organic fraction sample as co-substrate. These assays were divided into two groups, in which different conditions were applied. Group I investigated the variation in two operational parameters, i.e. the organic loading rate (OLR) and the hydraulic retention time (HRT), while in Group II, the assays were fed with different substrates in a sequential order...
June 22, 2017: Waste Management
https://www.readbyqxmd.com/read/28648745/batch-anaerobic-digestion-of-deproteinated-malt-whisky-pot-ale-using-different-source-inocula
#15
Raquel Barrena, Julio E Traub, Cristina Rodriguez Gil, Julian A S Goodwin, Alan J Harper, Nik A Willoughby, Antoni Sánchez, Thomas J Aspray
A novel process has been developed for the selective removal of protein from pot ale with recovered protein holding potential as a value-added by-product for the whisky industry. The purpose of this work was to assess the effect of deproteination on pot ale physicochemical characterisation and anaerobic digestion (AD) treatment. Pot ales were taken from five malt whisky distilleries and tested untreated, after centrifugation/filtration and after deproteination at laboratory or pilot scale. At laboratory scale, the deproteination process removed around 20% of total chemical oxygen demand (tCOD) from untreated pot ale and at least 30% dissolved copper from centrifuged pot ale...
June 22, 2017: Waste Management
https://www.readbyqxmd.com/read/28648369/water-diversion-projects-negatively-impact-lake-metabolism-a-case-study-in-lake-dazong-china
#16
Xiaolong Yao, Lu Zhang, Yunlin Zhang, Yingyang Du, Xingyu Jiang, Min Li
Water diversion projects are one of the major tools for counteracting water resource shortage in China. Many diversion projects aimed at diverting water from the Yangtze River or other rivers. This study, using one of the lakes receiving Yangtze River water as a case study, illustrated how nutrient, O2, and N2 fluxes across the sediment-water interface (SWI) changed with increasing nitrate loads during in situ benthic chamber incubation. Increasing nitrate loads in the overlying water caused complex changes in the SWI fluxes...
June 22, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28648180/kinetic-study-on-anaerobic-oxidation-of-methane-coupled-to-denitrification
#17
Hou Yu, Hiroyuki Kashima, John M Regan, Abid Hussain, Elsayed Elbeshbishy, Hyung-Sool Lee
Monod kinetic parameters provide information required for kinetic analysis of anaerobic oxidation of methane coupled to denitrification (AOM-D). This information is critical for engineering AOM-D processes in wastewater treatment facilities. We first experimentally determined Monod kinetic parameters for an AOM-D enriched culture and obtained the following values: maximum specific growth rate (μmax) 0.121/d, maximum substrate-utilization rate (qmax) 28.8mmol CH4/g cells-d, half maximum-rate substrate concentration (Ks) 83μΜ CH4, growth yield (Y) 4...
September 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28647695/the-warburg-effect-and-mass-spectrometry-based-proteomic-analysis
#18
REVIEW
Weidong Zhou, Lance A Liotta, Emanuel F Petricoin
Compared to normal cells, cancer cells have a unique metabolism by performing lactic acid fermentation in the presence of oxygen, also known as the Warburg effect. Researchers have proposed several hypotheses to elucidate the phenomenon, but the mechanism is still an enigma. In this review, we discuss three typical models, such as "damaged mitochondria", "adaptation to hypoxia", and "cell proliferation requirement", as well as contributions from mass spectrometry analysis toward our understanding of the Warburg effect...
July 2017: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/28647463/the-structurally-unique-photosynthetic-chlorella-variabilis-nc64a-hydrogenase-does-not-interact-with-plant-type-ferredoxins
#19
Vera Engelbrecht, Patricia Rodríguez-Maciá, Julian Esselborn, Anne Sawyer, Anja Hemschemeier, Olaf Rüdiger, Wolfgang Lubitz, Martin Winkler, Thomas Happe
Hydrogenases from green algae are linked to the photosynthetic electron transfer chain via the plant-type ferredoxin PetF. In this work the [FeFe]-hydrogenase from the Trebouxiophycean alga Chlorella variabilis NC64A (CvHydA1), which in contrast to other green algal hydrogenases contains additional FeS-cluster binding domains, was purified and specific enzyme activities for both hydrogen (H2) production and H2 oxidation were determined. Interestingly, although C. variabilis NC64A, like many Chlorophycean algal strains, exhibited light-dependent H2 production activity upon sulfur deprivation, CvHydA1 did not interact in vitro with several plant-type [2Fe-2S]-ferredoxins, but only with a bacterial2[4Fe4S]-ferredoxin...
June 21, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28647320/an-integrated-aerobic-anaerobic-strategy-for-performance-enhancement-of-pseudomonas-aeruginosa-inoculated-microbial-fuel-cell
#20
Xiao-Yu Yong, Zhi-Ying Yan, Hai-Bo Shen, Jun Zhou, Xia-Yuan Wu, Li-Juan Zhang, Tao Zheng, Min Jiang, Ping Wei, Hong-Hua Jia, Yang-Chun Yong
Microbial fuel cell (MFC) is a promising device for energy generation and organic waste treatment simultaneously by electrochemically active bacteria (EAB). In this study, an integrated aerobic-anaerobic strategy was developed to improve the performance of P. aeruginosa-inoculated MFC. With an aerobic start-up and following an anaerobic discharge process, the current density of MFC reached a maximum of 99.80µA/cm(2), which was 91.6% higher than the MFC with conventional constant-anaerobic operation. Cyclic voltammetry and HPLC analysis showed that aerobic start-up significantly increased electron shuttle (pyocyanin) production (76% higher than the constant-anaerobic MFC)...
June 12, 2017: Bioresource Technology
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