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https://www.readbyqxmd.com/read/29232639/tracking-variations-of-fluorescent-dissolved-organic-matter-during-wastewater-treatment-by-accumulative-fluorescence-emission-spectroscopy-combined-with-principal-component-second-derivative-and-canonical-correlation-analyses
#1
Xujing Guo, Huibin Yu, Zongcheng Yan, Hongjie Gao, Yizhang Zhang
Accumulative fluorescence emission (AFE) spectroscopy combined with principal component analysis (PCA), second derivative and canonical correlation analysis (CCA) was firstly developed into an available tool to track variations in dissolved organic matter (DOM) fractions and contents during wastewater treatment. Samples were collected from a wastewater treatment plant with a traditional anaerobic/anoxic/oxic (A2O) process. The AFE spectroscopy deduced from the sum of intensities along the excitation wavelengths of fluorescence excitation emission matrix (EEM), could distinctly track tyrosine-like, tryptophan-like, fulvic-like substances...
December 6, 2017: Chemosphere
https://www.readbyqxmd.com/read/29231915/lactobacillus-gasseri-endocarditis-on-the-aortic-valve-bioprosthesis-a-case-report
#2
Waldemar Elikowski, Małgorzata Małek-Elikowska, Monika Lisiecka, Anna Bodora, Katarzyna Wiśniewska, Zofia Oko-Sarnowska
Lactobacillus is a genus of Gram-positive, facultative anaerobic/ microaerophilic, rod-shaped bacteria. Lactobacilli constitute a significant component of the human microbiota in the oral cavity, gastrointestinal tract and female urogenital tract. They are also widely used as probiotics. Rarely, especially in patients with impaired immunity or with structural heart disease, do Lactobacilli become the pathogen responsible for serious infections, e.g. infective endocarditis (IE). CASE REPORT: The authors describe a case of an 80-year-old female with a past history of aortic valve replacement, mitral annuloplasty and pacemaker implantation admitted to hospital due to weakening, subfebrile state and chills...
November 23, 2017: Polski Merkuriusz Lekarski: Organ Polskiego Towarzystwa Lekarskiego
https://www.readbyqxmd.com/read/29231875/live-faecalibacterium-prausnitzii-does-not-enhance-epithelial-barrier-integrity-in-an-apical-anaerobic-co-culture-model-of-the-large-intestine
#3
Eva Maier, Rachel C Anderson, Nicole C Roy
Appropriate intestinal barrier maturation during infancy largely depends on colonization with commensal bacteria. Faecalibacterium prausnitzii is an abundant obligate anaerobe that colonizes during weaning and is thought to maintain colonic health throughout life. We previously showed that F. prausnitzii induced Toll-like receptor 2 (TLR2) activation, which is linked to enhanced tight junction formation. Therefore, we hypothesized that F. prausnitzii enhances barrier integrity, an important factor in appropriate intestinal barrier maturation...
December 12, 2017: Nutrients
https://www.readbyqxmd.com/read/29231866/the-consequences-of-being-in-an-infectious-biofilm-microenvironmental-conditions-governing-antibiotic-tolerance
#4
REVIEW
Majken Sønderholm, Thomas Bjarnsholt, Maria Alhede, Mette Kolpen, Peter Ø Jensen, Michael Kühl, Kasper N Kragh
The main driver behind biofilm research is the desire to understand the mechanisms governing the antibiotic tolerance of biofilm-growing bacteria found in chronic bacterial infections. Rather than genetic traits, several physical and chemical traits of the biofilm have been shown to be attributable to antibiotic tolerance. During infection, bacteria in biofilms exhibit slow growth and a low metabolic state due to O₂ limitation imposed by intense O₂ consumption of polymorphonuclear leukocytes or metabolically active bacteria in the biofilm periphery...
December 12, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29230966/transcriptome-analysis-and-anaerobic-c4-dicarboxylate-transport-in-actinobacillus-succinogenes
#5
Mi Na Rhie, Byeonghyeok Park, Hyeok-Jin Ko, In-Geol Choi, Ok Bin Kim
A global transcriptome analysis of the natural succinate producer Actinobacillus succinogenes revealed that 353 genes were differentially expressed when grown on various carbon and energy sources, which were categorized into six functional groups. We then analyzed the expression pattern of 37 potential C4 -dicarboxylate transporters in detail. A total of six transporters were considered potential fumarate transporters: three transporters, Asuc_1999 (Dcu), Asuc_0304 (DASS), and Asuc_0270-0273 (TRAP), were constitutively expressed, whereas three others, Asuc_1568 (DASS), Asuc_1482 (DASS), and Asuc_0142 (Dcu), were differentially expressed during growth on fumarate...
December 12, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/29230797/the-association-of-pre-operative-home-accelerometry-with-cardiopulmonary-exercise-variables
#6
H W Cui, G S Kirby, K Surmacz, C Hargrove, J Griffiths, B W Turney
We investigated the association of pre-operative activity, reported by the Duke Activity Score Index, Short Form-12 and measured by an accelerometer worn at home, with five cardiopulmonary exercise variables: peak power; peak oxygen consumption; anaerobic threshold; and ventilatory equivalents for oxygen and carbon dioxide. Fifty patients scheduled for major surgery underwent a standard pre-operative cardiopulmonary exercise test and wore a chest-mounted triaxial accelerometer for a mean (SD) duration of 3...
December 12, 2017: Anaesthesia
https://www.readbyqxmd.com/read/29230684/imipenem-relebactam-and-meropenem-vaborbactam-two-novel-carbapenem-%C3%AE-lactamase-inhibitor-combinations
#7
REVIEW
George G Zhanel, Courtney K Lawrence, Heather Adam, Frank Schweizer, Sheryl Zelenitsky, Michael Zhanel, Philippe R S Lagacé-Wiens, Andrew Walkty, Andrew Denisuik, Alyssa Golden, Alfred S Gin, Daryl J Hoban, Joseph P Lynch, James A Karlowsky
Relebactam (formerly known as MK-7655) is a non-β-lactam, bicyclic diazabicyclooctane, β-lactamase inhibitor that is structurally related to avibactam, differing by the addition of a piperidine ring to the 2-position carbonyl group. Vaborbactam (formerly known as RPX7009) is a non-β-lactam, cyclic, boronic acid-based, β-lactamase inhibitor. The structure of vaborbactam is unlike any other currently marketed β-lactamase inhibitor. Both inhibitors display activity against Ambler class A [including extended-spectrum β-lactamases (ESBLs), Klebsiella pneumoniae carbapenemases (KPCs)] and class C β-lactamases (AmpC)...
December 12, 2017: Drugs
https://www.readbyqxmd.com/read/29230577/long-term-adaptation-of-escherichia-coli-to-methanogenic-co-culture-enhanced-succinate-production-from-crude-glycerol
#8
Nam Yeun Kim, Su Nyung Kim, Ok Bin Kim
Escherichia coli can hardly grow anaerobically on glycerol without exogenous electron acceptor. The formate-consuming methanogen Methanobacterium formicicum plays a role as a living electron acceptor in glycerol fermentation of E. coli. Wild-type and mutant E. coli strains were screened for succinate production using glycerol in a co-culture with M. formicicum. Subsequently, E. coli was adapted to glycerol fermentation over 39 rounds (273 days) by successive co-culture with M. formicicum. The adapted E. coli (19...
December 12, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/29230528/cofactor-f430-as-a-biomarker-for-methanogenic-activity-application-to-an-anaerobic-bioreactor-system
#9
I Passaris, P Van Gaelen, R Cornelissen, K Simoens, D Grauwels, L Vanhaecke, D Springael, I Smets
Over the last decades, anaerobic bioreactor technology proved to be a competitive technology for purifying wastewater while producing biogas. Methanogens perform the crucial final step in methane production, and monitoring their activity is of paramount importance for system understanding and management. Cofactor F430 is an essential prosthetic group of the methyl-coenzyme M reductase (MCR) enzyme catalysing this final step. This research investigates whether the quantification of cofactor F430 in bioreactor systems is a viable intermediate-complexity monitoring tool in comparison to the conventional biogas and volatile fatty acid (VFA) concentration follow-up and molecular genetic techniques targeting the mcrA gene encoding the MCR protein or its transcripts...
December 11, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29230206/anaerobic-corrosion-of-304-stainless-steel-caused-by-the-pseudomonas-aeruginosa-biofilm
#10
Ru Jia, Dongqing Yang, Dake Xu, Tingyue Gu
Pseudomonas aeruginosa is a ubiquitous bacterium capable of forming problematic biofilms in many environments. They cause biocorrosion of medical implants and industrial equipment and infrastructure. Aerobic corrosion of P. aeruginosa against stainless steels has been reported by some researchers while there is a lack of reports on anaerobic P. aeruginosa corrosion in the literature. In this work, the corrosion by a wild-type P. aeruginosa (strain PAO1) biofilm against 304 stainless steel (304 SS) was investigated under strictly anaerobic condition for up to 14 days...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29228768/recovery-of-mrs3%C3%AE-mrs4%C3%AE-saccharomyces-cerevisiae-cells-under-iron-sufficient-conditions-and-the-role-of-fe580
#11
Michael J Moore, Joshua Wofford, Andrew Dancis, Paul Alan Lindahl
Mrs3 and Mrs4 are mitochondrial inner membrane proteins that deliver an unidentified cytosolic iron species into the matrix for use in iron-sulfur-cluster (ISC) and heme biosynthesis. The Mrs3/4 double-deletion strain (ΔΔ) grew slowly in iron-deficient glycerol/ethanol medium but recovered to WT rates in iron-sufficient medium. ΔΔ cells grown under both iron-deficient and iron-sufficient respiring conditions accumulated large amounts of iron relative to WT cells, indicating iron homeostatic dysregulation regardless of nutrient iron status...
December 11, 2017: Biochemistry
https://www.readbyqxmd.com/read/29227859/waste-biorefineries-integrating-anaerobic-digestion-and-microalgae-cultivation-for-bioenergy-production
#12
REVIEW
Yi-di Chen, Shih-Hsin Ho, Dillirani Nagarajan, Nan-Qi Ren, Jo-Shu Chang
Commercialization of microalgal cultivation has been well realized in recent decades with the use of effective strains that can yield the target products, but it is still challenged by the high costs arising from mass production, harvesting, and further processing. Recently, more interest has been directed towards the utilization of waste resources, such as sludge digestate, to enhance the economic feasibility and sustainability of microalgae production. Anaerobic digestion for waste disposal and phototrophic microalgal cultivation are well-characterized technologies in both fields...
December 8, 2017: Current Opinion in Biotechnology
https://www.readbyqxmd.com/read/29227730/physical-and-performance-characteristics-related-to-unintentional-musculoskeletal-injury-in-special-forces-operators-a-prospective-analysis
#13
Nicholas R Heebner, John P Abt, Mita Lovalekar, Kim Beals, Timothy C Sell, Jeffery Morgan, Shawn Kane, Scott Lephart
CONTEXT:   Seventy-seven percent of musculoskeletal injuries sustained by United States Army Special Forces Operators are preventable. Identification of predictive characteristics will promote the development of screening methods to augment injury-prevention programs. OBJECTIVE:   To determine physical and performance characteristics that predict musculoskeletal injuries. SETTING:   Clinical laboratory. PATIENTS OR OTHER PARTICIPANTS:   A total of 95 Operators (age = 32...
December 11, 2017: Journal of Athletic Training
https://www.readbyqxmd.com/read/29226760/the-diagnostic-performance-of-the-beta-glucan-assay-in-the-detection-of-intra-amniotic-infection-with-candida-species
#14
Percy Pacora, Roberto Romero, Offer Erez, Eli Maymon, Bogdan Panaitescu, Juan Pedro Kusanovic, Adi L Tarca, Chaur-Dong Hsu, Sonia S Hassan
INTRODUCTION: A bioassay based on the detection of beta-glucan, a constituent of the cell wall of fungi, has been successfully used to diagnose fungal infections in a variety of biological fluids but not yet in the amniotic fluid. OBJECTIVE: To determine the diagnostic performance of a beta-glucan bioassay in the detection of Candida species in the amniotic fluid. METHODS: The study population comprised women who had a singleton pregnancy without congenital or chromosomal abnormalities, who experienced preterm labor or preterm prelabor rupture of the fetal membranes, and who underwent transabdominal amniocentesis for clinical indications...
December 10, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/29226713/-prognostic-importance-of-lactate-dehydrogenase-ldh-in-oncology
#15
Dániel Deme, András Telekes
Glycolysis is increased in most of the malignant cells, providing the largest proportion of energy needed for cell proliferation. Lactate dehydrogenase (LDH) catalyses the reversible process of pyruvate to lactate in anaerobic condition. LDHA isoenzyme expressed mainly by malignant cells, significantly increases lactate formation. Lactate induces the proliferation of oxygenated malignant cells, angiogenesis, and inhibits the innate and adaptive immune responses. Baseline serum LDH elevation correlates with shorter survival...
December 2017: Orvosi Hetilap
https://www.readbyqxmd.com/read/29226074/characterization-of-auxiliary-iron-sulfur-clusters-in-a-radical-s-adenosylmethionine-enzyme-pqqe-from-methylobacterium-extorquens-am1
#16
Natsaran Saichana, Katsuyuki Tanizawa, Hiroshi Ueno, Jiří Pechoušek, Petr Novák, Jitka Frébortová
PqqE is a radical S-adenosyl-l-methionine (SAM) enzyme that catalyzes the initial reaction of pyrroloquinoline quinone (PQQ) biosynthesis. PqqE belongs to the SPASM (subtilosin/PQQ/anaerobic sulfatase/mycofactocin maturating enzymes) subfamily of the radical SAM superfamily and contains multiple Fe-S clusters. To characterize the Fe-S clusters in PqqE from Methylobacterium extorquens AM1, Cys residues conserved in the N-terminal signature motif (CX 3 CX 2C) and the C-terminal seven-cysteine motif (CX 9-15 GX 4 CX n CX 2 CX 5 CX 3 CX n C; n = an unspecified number) were individually or simultaneously mutated into Ser...
December 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/29225380/performance-of-anaerobic-biotrickling-filter-and-its-microbial-diversity-for-the-removal-of-stripped-disinfection-byproducts
#17
Bineyam Mezgebe, George A Sorial, E Sahle-Demessie, Ashraf Aly Hassan, Jingrang Lu
The objective of this research was to evaluate the biodegradation of chloroform by using biotrickling filter (BTF) and determining the dominant bacteria responsible for the degradation. The research was conducted in three phases under anaerobic condition, namely, in the presence of co-metabolite (Phase I), in the presence of co-metabolite and surfactant (Phase II) and in the presence of surfactant but no co-metabolite (Phase III). The results showed that the presence of ethanol as a co-metabolite provided 49% removal efficiency...
November 1, 2017: Water, Air, and Soil Pollution
https://www.readbyqxmd.com/read/29225287/lysogenisation-of-shiga-toxin-encoding-bacteriophage-represses-cell-motility
#18
Shoichi Mitsunaka, Naoki Sudo, Yasuhiko Sekine
Bacteriophages are genetic elements that play key roles in the evolution and diversification of bacterial genomes. The Shiga toxin (Stx)-encoding phage plays an important role in the horizontal transfer of the stx gene. However, the influence of the Stx phage integration on the physiological properties and gene expression pattern of the host have not been clearly resolved. In this study, we constructed the Sp5 lysogen through lysogenisation of E. coli K-12 by Sp5, an Stx2 phage in enterohaemorrhagic E. coli (EHEC) O157:H7 Sakai, and examined the effect of the resulting lysogen on cell motility under various growth conditions...
December 8, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/29224929/anaerobic-biodegradation-of-dissolved-ethanol-in-a-pilot-scale-sand-aquifer-variability-in-plume-redox-biogeochemistry
#19
Heather C McLeod, James W Roy, Gregory F Slater, James E Smith
The use of ethanol in alternative fuels has led to contamination of groundwater with high concentrations of this easily biodegradable organic compound. Previous laboratory and field studies have shown vigorous biodegradation of ethanol plumes, with prevalence of reducing conditions and methanogenesis. The objective of this study was to further our understanding of the dynamic biogeochemistry processes, especially dissolved gas production, that may occur in developing and aging plume cores at sites with ethanol or other organic contamination of groundwater...
December 5, 2017: Journal of Contaminant Hydrology
https://www.readbyqxmd.com/read/29224872/greenhouse-gas-emissions-from-liquid-dairy-manure-prediction-and-mitigation
#20
Søren O Petersen
The handling and use of manure on livestock farms contributes to emissions of the greenhouse gases (GHG) CH4 and N2O, especially with liquid manure management. Dairy farms are diverse with respect to manure management, with practices ranging from daily spreading to long-term storage for more efficient recycling of manure nutrients for crop production. Opportunities for GHG mitigation will depend on the baseline situation with respect to handling and storage, and therefore prediction and mitigation at the farm level requires a dynamic description of housing systems and storage conditions, and use of treatment technologies...
December 7, 2017: Journal of Dairy Science
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