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https://www.readbyqxmd.com/read/29054061/microbial-characteristics-analysis-and-kinetic-studies-on-substrate-composition-to-methane-after-microbial-and-nutritional-regulation-of-fruit-and-vegetable-wastes-anaerobic-digestion
#1
Chunhui Zhao, Hui Mu, Yuxiao Zhao, Liguo Wang, Bin Zuo
This study firstly evaluated the microbial role when choosing the acclimated anaerobic granular sludge (AGS) and waste activated sludge (WAS) as microbial and nutritional regulators to improve the biomethanation of fruit and vegetable wastes (FVW). Results showed that the enriched hydrogenotrophic methanogens, and Firmicutes and Spirochaeta in the AGS were responsible for the enhanced methane yield. A synthetic waste representing the mixture of WAS and FVW was then used to investigate the influences of different substrate composition on methane generations...
October 10, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29054059/enhancement-of-sludge-reduction-by-ultrasonic-pretreatment-and-packing-carriers-in-the-anaerobic-side-stream-reactor-performance-sludge-characteristics-and-microbial-community-structure
#2
Cheng Cheng, Zhen Zhou, Zhan Qiu, Jiyuan Yang, Wei Wu, Hongjian Pang
Effects of ultrasonic pretreatment and packing carriers on sludge reduction, settleability, dewaterability and microbial community structure in the anaerobic side-stream reactor (ASSR) were investigated with three anaerobic reactors operated in parallel. Ultrasonication from 3.65% in the ASSR to 5.08%, and packing carriers further enhanced the efficiency to 19.2%. Ultrasonic pretreatment of sludge decreased oxidation-reduction potential in ASSR and enhanced the release of intracellular substances. The deterioration of sludge settleability and dewaterability in the ASSR after ultrasonic pretreatment was improved by packing carriers...
October 10, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29054058/study-on-improving-anaerobic-co-digestion-of-cow-manure-and-corn-straw-by-fruit-and-vegetable-waste-methane-production-and-microbial-community-in-cstr-process
#3
Xuemei Wang, Zifu Li, Xue Bai, Xiaoqin Zhou, Sikun Cheng, Ruiling Gao, Jiachen Sun
Based on continuous anaerobic co-digestion of cow manure with available carbon slowly released corn straw, the effect of adding available carbon quickly released fruit and vegetable waste (FVW) was explored, meanwhile microbial community variation was studied in this study. When the FVW added was 5% and 1%, the methane production of the cow manure and corn straw was improved, and the start-up process was shortened. With higher proportion of FVW to 5%, the performance was superior with a mean methane yield increase of 22...
October 10, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29053985/rapid-cultivation-of-free-living-planktonic-anammox-cells
#4
Lei Zhang, Satoshi Okabe
Despite that anaerobic ammonium oxidizing (anammox) bacteria are key players in the global nitrogen cycle, no pure cultures are still available. Planktonic cell culture with high purity is, therefore, essential for physiological and biochemical studies of anammox bacteria. However, development of such planktonic cell cultures requires an enormous amount of time and effort. Here we developed a novel rapid method for cultivating free-living planktonic anammox cells. First, anammox granules were physically dispersed, immobilized in 6% polyvinyl alcohol-4% sodium alginate (PVA-SA) gel beads, and then pre-cultured in an up-flow column reactor...
October 15, 2017: Water Research
https://www.readbyqxmd.com/read/29053957/cdk4-phosphorylates-ampk%C3%AE-2-to-inhibit-its-activity-and-repress-fatty-acid-oxidation
#5
Isabel C Lopez-Mejia, Sylviane Lagarrigue, Albert Giralt, Laia Martinez-Carreres, Nadège Zanou, Pierre-Damien Denechaud, Judit Castillo-Armengol, Carine Chavey, Meritxell Orpinell, Brigitte Delacuisine, Anita Nasrallah, Caterina Collodet, Lianjun Zhang, Benoît Viollet, D Grahame Hardie, Lluis Fajas
The roles of CDK4 in the cell cycle have been extensively studied, but less is known about the mechanisms underlying the metabolic regulation by CDK4. Here, we report that CDK4 promotes anaerobic glycolysis and represses fatty acid oxidation in mouse embryonic fibroblasts (MEFs) by targeting the AMP-activated protein kinase (AMPK). We also show that fatty acid oxidation (FAO) is specifically induced by AMPK complexes containing the α2 subunit. Moreover, we report that CDK4 represses FAO through direct phosphorylation and inhibition of AMPKα2...
October 19, 2017: Molecular Cell
https://www.readbyqxmd.com/read/29053751/bacterial-community-analysis-of-anoxic-aeration-a-o-system-in-a-combined-process-for-gibberellin-wastewater-treatment
#6
Erming Ouyang, Yao Lu, Jiating Ouyang, Lele Wang, Xiaohui Wang
Gibberellin wastewater cannot be directly discharged without treatment due to its high concentrations of sulfate and organic compounds and strong acidity. Therefore, multi-stage anaerobic bioreactor + micro-aerobic+ anoxic/aeration (A/O) + biological contact oxidation combined processes are used to treat gibberellin wastewater. However, knowledge of the treatment effects of the A/O process and bacterial community structure in the aeration tank reactors of such systems is sparse. Therefore, this study was conducted to investigate the treatment effects and operation of the A/O process on gibberellin wastewater, as well as changes in the bacterial community structure of activated sludge in the aeration tank during treatment...
2017: PloS One
https://www.readbyqxmd.com/read/29053417/hif-1%C3%AE-promotes-breast-cancer-cell-mcf-7-proliferation-and-invasion-through-regulating-mir-210
#7
Yang Zhang, Jinyin Yan, Lu Wang, Hao Dai, Ning Li, Wanning Hu, Haifeng Cai
OBJECTIVE: Many malignant tumors grow in hypoxic condition, which is associated with tumor growth, invasion, and metastasis. MicroRNAs are of great significance in the development of multiple malignant tumors. This study cultured breast cancer cell MCF-7 under the condition of different concentrations of oxygen, to test cell proliferation and invasion, and detect miR-210 expression, aiming to analyze the influence of hypoxia on breast cancer cell behaviors as well as miR-210 expressions...
October 2017: Cancer Biotherapy & Radiopharmaceuticals
https://www.readbyqxmd.com/read/29053273/distinguishing-nitro-vs-nitrito-coordination-in-cytochrome-c-using-vibrational-spectroscopy-and-density-functional-theory
#8
Zach N Nilsson, Brian L Mandella, Kakali Sen, Demet Kekilli, Michael A Hough, Pierre Moënne-Loccoz, Richard W Strange, Colin R Andrew
Nitrite coordination to heme cofactors is a key step in the anaerobic production of the signaling molecule nitric oxide (NO). An ambidentate ligand, nitrite has the potential to coordinate via the N- (nitro) or O- (nitrito) atoms in a manner that can direct its reactivity. Distinguishing nitro vs nitrito coordination, along with the influence of the surrounding protein, is therefore of particular interest. In this study, we probed Fe(III) heme-nitrite coordination in Alcaligenes xylosoxidans cytochrome c' (AXCP), an NO carrier that excludes anions in its native state but that readily binds nitrite (Kd ∼ 0...
October 20, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/29053066/different-times-of-day-do-not-change-heart-rate-variability-recovery-after-light-exercise-in-sedentary-subjects-24%C3%A2-hours-holter-monitoring
#9
Eliza Prodel, Tiago Peçanha, Lilian Pinto Da Silva, Rogério Baumgratz De Paula, Daniel Godoy Martinez, Jorge Roberto Perrout De Lima, Mateus Camaroti Laterza
Incidence of cardiovascular events follows a circadian rhythm with peak occurrence during morning. Disturbance of autonomic control caused by exercise had raised the question of the safety in morning exercise and its recovery. Furthermore, we sought to investigate whether light aerobic exercise performed at night would increase HR and decrease HRV during sleep. Therefore, the aim of this study was to test the hypothesis that morning exercise would delay HR and HRV recovery after light aerobic exercise, additionally, we tested the impact of late night light aerobic exercise on HR and HRV during sleep in sedentary subjects...
October 20, 2017: Chronobiology International
https://www.readbyqxmd.com/read/29051020/draft-genome-sequences-of-three-multidrug-resistant-cutibacterium-formerly-propionibacterium-acnes-strains-isolated-from-acne-patients-china
#10
Nanxue Zhang, Zhong Lu, Ying Ma
OBJECTIVES: Cutibacterium acnes (formerly Propionibacterium acnes), an anaerobic Gram-positive bacterium, is a skin commensal and considered to be associated with acne. Here we report draft genome sequences of three multidrug-resistant C. acnes strains (CA17, CA39, and CA51), isolated from skin lesions of acne patients from China. METHODS: The whole genome of the three multidrug-resistant C. acnes strains were sequenced using an Illumina HiSeq 2500 platform. De novo assembly and annotation were performed with SPAdes-3...
October 16, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29050796/sites-of-infection-associated-with-streptococcus-anginosus-group-among-children
#11
Mihoko Furuichi, Yuho Horikoshi
Streptococcus anginosus group (SAG) are parts of normal flora of the oral cavity and associated with abscess forming in various sites on the body. Although the clinical features of infections caused by each member of the SAG in adults has been reported, it has not well been known in children. The aim of this study was to clarify the site of infections associated with individual SAG species among children. Medical records from March 2010 to July 2016 were reviewed at Tokyo Metropolitan Children's Medical Center...
October 17, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/29050652/gm-1-n-method-for-the-prediction-of-anaerobic-digestion-system-and-sensitivity-analysis-of-influential-factors
#12
Jingzheng Ren
Anaerobic digestion process has been recognized as a promising way for waste treatment and energy recovery in a sustainable way. Modelling of anaerobic digestion system is significantly important for effectively and accurately controlling, adjusting, and predicting the system for higher methane yield. The GM(1,N) approach which does not need the mechanism or a large number of samples was employed to model the anaerobic digestion system to predict methane yield. In order to illustrate the proposed model, an illustrative case about anaerobic digestion of municipal solid waste for methane yield was studied, and the results demonstrate that GM(1,N) model can effectively simulate anaerobic digestion system at the cases of poor information with less computational expense...
October 10, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29050018/root-surface-biofilms-and-caries
#13
Thuy Do, Nailê Damé-Teixeira, Monika Naginyte, Philip D Marsh
Following gingival recession, which increases with age, the root surface becomes exposed, creating new environments for microbial colonization and biofilm formation. The formation of root surface biofilms is influenced by the availability and composition of saliva and gingival crevicular fluid; they provide components for the conditioning film (acquired root surface pellicle) and also act as a source of nutrients. The early bacterial colonizers of the root surface are similar to those found on the enamel, and Gram-positive species such as Streptococcus sanguinis, S...
2017: Monographs in Oral Science
https://www.readbyqxmd.com/read/29049966/performance-comparison-of-secondary-and-tertiary-treatment-systems-for-treating-antibiotic-resistance
#14
Manisha Lamba, Shaikh Ziauddin Ahammad
Rapid emergence of antibiotic resistance (AR) in developing countries is posing a greater health risk and increasing the global disease burden. AR proliferation mediated by treated/untreated discharges from sewage treatment plants (STPs) is a prime public health concern. Efficient sewage treatment is among our key defenses against the dissemination of infectious diseases. The present study aims to estimate the efficiency of aerobic [activated sludge process (ASP) and modified trickling filter (MTF)] and anaerobic reactors (anaerobic flow-through reactor) along with the three disinfection techniques (UV, ozone and chlorination) in reducing ARB and ARGs present in the domestic sewage...
October 12, 2017: Water Research
https://www.readbyqxmd.com/read/29049954/application-of-carnobacterium-maltaromaticum-as-a-feed-additive-for-weaned-rabbits-to-improve-meat-microbial-quality-and-safety
#15
Amenan Prisca Koné, Juliana Maria Velez Zea, Dominic Gagné, Dany Cinq-Mars, Frédéric Guay, Linda Saucier
This study addresses the improvement of meat microbial quality by enriching the diet of farm animals with a protective culture. Weaned Grimaud rabbits were divided into two experimental groups: a control and a diet supplemented with Micocin® (Carnobacterium maltaromaticum CB1; 8Log10CFU/kg of feed). Overall, meat quality was not affected substantially by the treatment. Total Aerobic Mesophilic (TAM), Escherichia coli and other coliforms, Enterobacteriaceae, Staphylococcus aureus, Pseudomonas spp., Listeria spp...
October 2, 2017: Meat Science
https://www.readbyqxmd.com/read/29049419/multi-time-series-rna-seq-analysis-of-enterobacter-lignolyticus-scf1-during-growth-in-lignin-amended-medium
#16
Roberto Orellana, Gina Chaput, Lye Meng Markillie, Hugh Mitchell, Matt Gaffrey, Galya Orr, Kristen M DeAngelis
The production of lignocellulosic-derived biofuels is a highly promising source of alternative energy, but it has been constrained by the lack of a microbial platform capable to efficiently degrade this recalcitrant material and cope with by-products that can be toxic to cells. Species that naturally grow in environments where carbon is mainly available as lignin are promising for finding new ways of removing the lignin that protects cellulose for improved conversion of lignin to fuel precursors. Enterobacter lignolyticus SCF1 is a facultative anaerobic Gammaproteobacteria isolated from tropical rain forest soil collected in El Yunque forest, Puerto Rico under anoxic growth conditions with lignin as sole carbon source...
2017: PloS One
https://www.readbyqxmd.com/read/29046741/complete-genome-sequence-of-thermotoga-sp-strain-rq7
#17
Zhaohui Xu, Rutika Puranik, Junxi Hu, Hui Xu, Dongmei Han
Thermotoga sp. strain RQ7 is a member of the family Thermotogaceae in the order Thermotogales. It is a Gram negative, hyperthermophilic, and strictly anaerobic bacterium. It grows on diverse simple and complex carbohydrates and can use protons as the final electron acceptor. Its complete genome is composed of a chromosome of 1,851,618 bp and a plasmid of 846 bp. The chromosome contains 1906 putative genes, including 1853 protein coding genes and 53 RNA genes. The genetic features pertaining to various lateral gene transfer mechanisms are analyzed...
2017: Standards in Genomic Sciences
https://www.readbyqxmd.com/read/29046740/draft-genome-sequence-and-characterization-of-commensal-escherichia-coli-strain-bg1-isolated-from-bovine-gastro-intestinal-tract
#18
Audrey Segura, Pauline Auffret, Christophe Klopp, Yolande Bertin, Evelyne Forano
Escherichia coli is the most abundant facultative anaerobic bacteria in the gastro-intestinal tract of mammals but can be responsible for intestinal infection due to acquisition of virulence factors. Genomes of pathogenic E. coli strains are widely described whereas those of bovine commensal E. coli strains are very scarce. Here, we report the genome sequence, annotation, and features of the commensal E. coli BG1 isolated from the gastro-intestinal tract of cattle. Whole genome sequencing analysis showed that BG1 has a chromosome of 4,782,107 bp coding for 4465 proteins and 97 RNAs...
2017: Standards in Genomic Sciences
https://www.readbyqxmd.com/read/29046168/combined-treatment-of-mezcal-vinasses-by-ozonation-and-activated-sludge
#19
(no author information available yet)
In Mexico, mezcal production generates huge amounts of vinasses (MV) that cause negative environmental impacts. Thus, MV treatment is necessary before discharge to water bodies. Although there is no information for mezcal vinasses, similar effluents have been treated by biological processes (i.e. anaerobic and aerobic) usually complemented by oxidative chemical pretreatments (ozonation) and physico-chemical methods. In this work MV were first ozonated and followed by batch aerobic biological degradation. In the ozonation stage, organic matter removals were 4...
October 18, 2017: Water Environment Research: a Research Publication of the Water Environment Federation
https://www.readbyqxmd.com/read/29045925/mixotrophic-chlorella-sp-uj-3-cultivation-in-the-typical-anaerobic-fermentation-effluents
#20
Shuhao Huo, Miao Kong, Feifei Zhu, Bin Zou, Feng Wang, Ling Xu, Cunsheng Zhang, Daming Huang
The growth of mixotrophic Chlorella sp. UJ-3 cultivated in the three typical anaerobic fermentation effluents was investigated in this paper. The results showed that the microalgae grew best under intermediate light intensity for all the types of fermentation effluents. The butyrate type fermentation effluents induced the fastest growth rate for Chlorella sp. UJ-3, with a maximal cell concentration of 3.8×10(7) cells/mL. Under intermediate light intensity, the volatile fatty acids (VFAs) were almost depleted on the fifth day of the cultivation for all the three types of fermentation systems...
October 10, 2017: Bioresource Technology
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