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https://www.readbyqxmd.com/read/27924608/a-method-to-isolate-purify-and-characterize-human-periodontal-ligament-stem-cells
#1
Krzysztof Mrozik, Stan Gronthos, Songtao Shi, P Mark Bartold
Human periodontal ligament stem cells (PDLSCs) are a unique population of mesenchymal stem cells (MSCs) that demonstrate the capacity to generate cementum- and periodontal ligament-like structures in vivo. As such, PDLSCs represent a promising cell-based therapy in reconstructive dentistry for the treatment of periodontal disease. The present chapter describes two methods for isolating PDLSCs from human PDL tissue including traditional plastic adherence, and immunomagnetic selection based on the expression of MSC-associated surface markers STRO-1 antigen, CD146 (MUC-18), CD29 (Integrin β-1), CD44, and CD106 (VCAM-1)...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27923222/pediatric-primary-diffuse-leptomeningeal-primitive-neuroectodermal-tumor-a-case-report-and-literature-review
#2
Jesna Mathew Sublett, Caitlin Davenport, Howard Eisenbrock, Shamsher Dalal, Syed A Jaffar Kazmi, Amir Kershenovich
BACKGROUND: Primary diffuse leptomeningeal primitive neuroectodermal tumor (PDL PNET) is extremely rare, with only 19 cases reported in the literature to date. We present a case of a child with rapidly progressive PDL PNET and a literature review. A 10-year-old boy presented with mood lability, hallucinations, generalized pain, enuresis, and headaches. Initial investigation failed to produce a diagnosis. The symptoms progressed to seizure, back pain, and papilledema. Imaging showed acute hydrocephalus and mild diffuse leptomeningeal enhancement without an identifiable primary lesion...
December 7, 2016: Pediatric Neurosurgery
https://www.readbyqxmd.com/read/27911476/nd-yag-and-pulsed-dye-laser-therapy-in-infantile-hemangiomas-a-retrospective-analysis-of-271-treated-hemangiomas-in-149-children
#3
F Hartmann, A Lockmann, L-L Grönemeyer, H A Haenssle, M Zutt, H von Fintel, I Kühnle, M P Schön, K-M Thoms
BACKGROUND: Infantile hemangiomas (IH) are common benign tumors in infancy. Most IH resolve spontaneously, but some require treatment due to ulceration, functional impairment or cosmetic disfiguration. While systemic propranolol is effective in many cases, laser therapy may be a safe topical alternative. OBJECTIVE: To assess the efficacy of combined Nd:YAG/pulsed dye laser (PDL) or PDL alone for therapy of IH. PATIENTS AND METHODS: 271 IH in 149 infants were treated with combined Nd:YAG/PDL or PDL alone...
December 2, 2016: Journal of the European Academy of Dermatology and Venereology: JEADV
https://www.readbyqxmd.com/read/27911144/comparison-of-pulsed-dye-laser-versus-combined-pulsed-dye-laser-and-nd-yag-laser-in-the-treatment-of-inflammatory-acne-vulgaris
#4
Manal Mohamed Salah El Din, Nevien Ahmed Samy, Amira Eid Salem
BACKGROUND: Both pulsed dye laser and combined 585/1,064-nm (sequential dual-wave-length PDL and Nd:YAG) laser improves inflammatory skin disorders including acne vulgaris. OBJECTIVE: To compare the efficacy of 585 nm pulsed dye laser versus sequential dual-wave-length PDL and Nd:YAG in treatment of acne vulgaris. PATIENTS AND METHOD: Thirty patients complaining of acne vulgaris were treated with a single pass of PDL alone on half of the face and a single pass of combined 585/1064-nm laser on the other half for four treatment sessions at 2 weeks interval, then assessed by the count and severity of the acne lesions & digital camera photographs Results: The study showed that inflammatory acne lesions count was significantly reduced by 82...
December 2, 2016: Journal of Cosmetic and Laser Therapy: Official Publication of the European Society for Laser Dermatology
https://www.readbyqxmd.com/read/27907187/substance-p-induces-ho-1-expression-in-raw-264-7-cells-promoting-switch-towards-m2-like-macrophages
#5
Giovanna Montana, Nadia Lampiasi
Substance P (SP) is a neuropeptide that mediates many physiological as well as inflammatory responses. Recently, SP has been implicated in the resolution of inflammation through induction of M2 macrophages phenotype. The shift between M1-like and M2-like, allowing the resolution of inflammatory processes, also takes place by means of hemeoxygenase-1 (HO-1). HO-1 is induced in response to oxidative stress and inflammatory stimuli and modulates the immune response through macrophages polarisation. SP induces HO-1 expression in human periodontal ligament (PDL), the latter potentially plays a role in cytoprotection...
2016: PloS One
https://www.readbyqxmd.com/read/27905816/cementocyte-cell-death-occurs-in-rat-cellular-cementum-during-orthodontic-tooth-movement
#6
Humihiro Matsuzawa, Naoko Toriya, Yuya Nakao, Moe Konno-Nagasaka, Toshiya Arakawa, Miki Okayama, Itaru Mizoguchi
OBJECTIVE: To clarify the mechanism of root resorption during orthodontic treatment, we examined cementocyte cell death and root resorption in the cellular cementum on the pressure side during experimental tooth movement. MATERIALS AND METHODS: Using 8-week-old male Wistar rats, the right first molar was pushed mesiobuccally with a force of 40 g by a Ni-Ti alloy wire while the contralateral first molar was used as a control. Localization and number of cleaved caspase-3-positive and single-stranded DNA (ssDNA) - positive cells were evaluated using dual-label immunohistochemistry with anticleaved caspase-3 and anti-ssDNA antibodies...
December 1, 2016: Angle Orthodontist
https://www.readbyqxmd.com/read/27901202/in-vivo-performance-of-different-scaffolds-for-dental-pulp-stem-cells-induced-for-odontogenic-differentiation
#7
Cigdem Atalayin, Huseyin Tezel, Taner Dagci, Nefise Ulku Karabay Yavasoglu, Gulperi Oktem, Timur Kose
This study was designed to determine the in vivo performance of three different materials as scaffolds for dental pulp stem cells (DPSC) undergoing induced odontogenic differentiation. An odontogenic medium modified by the addition of recombinant human bone morphogenetic protein 2 was used in the experimental groups to induce differentiation. Mesenchymal stem cell medium was used in the control groups. DPSC were transplanted onto the backs of mice via three scaffolds: copolymer of L-lactide and DL-lactide (PLDL), copolymer of DL-lactide (PDL) and hydroxyapatite tricalcium phosphate (HA/TCP)...
November 28, 2016: Brazilian Oral Research
https://www.readbyqxmd.com/read/27897460/current-evidence-on-the-socket-shield-technique-a-systematic-review
#8
Amit Srikant Gharpure, Neel B Bhatavadekar
The recently popularised socket shield technique involves intentional retention of a thin buccal section of the remnant root at the time of immediate implant placement to preserve the buccal crestal bone from resorption. The objective of this systematic review was to assess the literature available on the socket-shield technique and weigh its biological plausibility and long-term clinical prognosis. A Systematic Search was performed in PubMed-Medline, Embase, Web of Knowledge, Google Scholar and Cochrane Central for clinical/ animal studies up to May 2016 without restrictions of language, duration, and follow-up...
November 29, 2016: Journal of Oral Implantology
https://www.readbyqxmd.com/read/27893539/comparative-efficacy-of-radiofrequency-and-pulsed-dye-laser-in-the-treatment-of-rosacea
#9
Sue-Jeong Kim, Young Lee, Young-Joon Seo, Jeung-Hoon Lee, Myung Im
BACKGROUND: Laser and light-based therapies have been used successfully in the treatment of rosacea; however, evidence is lacking regarding the efficacy of radiofrequency (RF). OBJECTIVE: This study evaluated the efficacy of RF in the treatment of rosacea compared with pulsed dye laser (PDL). METHODS: Thirty patients with rosacea (erythematotelangiectatic rosacea [ETR], n = 20; papulopustular rosacea [PPR], n = 10) were enrolled in a randomized, controlled, split-face study...
November 23, 2016: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
https://www.readbyqxmd.com/read/27879157/secondary-submucosal-vocal-cyst-following-pulsed-dye-laser-application-for-sulcus-vocalis
#10
Joo Hyun Kim, Hye Jin Hwang, Ji Hyung Kim, Min Seok Kang, Hong-Shik Choi, Hyung Kwon Byeon
CONCLUSION: Submucosal vocal cyst can occur as a distinct long-term sequelae following pulsed dye laser (PDL) application for sulcus vocalis. It is recommended that the cyst lesion should be surgically removed for treatment. Optimal and precise irradiation of PDL is considered important to avoid the development of a such complication. OBJECTIVE: After several years of clinical practice and an extended period of follow-up of the patients who received PDL application in treating sulcus vocalis, the authors have encountered several cases of secondary submucosal vocal cyst development...
November 23, 2016: Acta Oto-laryngologica
https://www.readbyqxmd.com/read/27869377/physical-modalities-for-the-treatment-of-rosacea
#11
Maja A Hofmann, Percy Lehmann
Rosacea is characterised by a wide variety of vascular changes. Apart from telangiectasia and erythema, often so-called flushing occurs. These vascular abnormalities can be targeted with specific light and laser devices. In addition to KTP laser, pulsed dye laser (PDL) and Nd:YAG laser, also intense pulsed light devices (IPLs) are used. The described therapeutic effects include the reduction of vascular abnormalities and even improvement of papulopustular changes. While the KTP laser shows very good results in telangiectasia, the dye laser and IPL devices are used preferably in erythema...
December 2016: Journal der Deutschen Dermatologischen Gesellschaft, Journal of the German Society of Dermatology: JDDG
https://www.readbyqxmd.com/read/27869376/physikalische-methoden-zur-behandlung-der-rosazea
#12
Maja A Hofmann, Percy Lehmann
Die Rosazea ist durch vielfältige vaskuläre Veränderungen gekennzeichnet. Neben Teleangiektasien und Erythemen treten häufig auch sogenannte "Flushings" auf. Haupteinsatzgebiete von Licht- und Lasersystemen sind diese vaskulären Veränderungen, wie Erytheme und Teleangiektasien. Neben dem KTP-Laser, dem Farbstofflaser (PDL) und dem Nd:YAG-Laser kommen auch Blitzlampen (IPL) zum Einsatz. Neben der Rückbildung der vaskulären Komponente, ist auch eine Verbesserung der papulopustulösen Komponente beschrieben...
December 2016: Journal der Deutschen Dermatologischen Gesellschaft, Journal of the German Society of Dermatology: JDDG
https://www.readbyqxmd.com/read/27857352/magnetic-field-sensing-using-standard-uniform-fbgs
#13
F Descamps, D Kinet, S Bette, C Caucheteur
Magnetic field sensing can be directly (i.e. without requiring magnetic fuilds or magnetostrictive materials) obtained from the estimation of the circular birefringence induced in optical fibers through the so-called Faraday effect. In standard telecommunication-grade optical fiber, the amount of induced circular birefringence is however of the same order of the intrinsic fiber linear birefringence or even below. Hence, whenever uniform fiber Bragg gratings (FBGs) are used to probe this evolution, the resulting accuracy is usually very poor, even in the case of polarization-assisted measurements based on polarization dependent loss (PDL) or differential group delay (DGD)...
November 14, 2016: Optics Express
https://www.readbyqxmd.com/read/27832279/artificial-polymeric-scaffolds-as-extracellular-matrix-substitutes-for-autologous-conjunctival-goblet-cell-expansion
#14
Min He, Thomas Storr-Paulsen, Annie L Wang, Chiara E Ghezzi, Siran Wang, Matthew Fullana, Dimitrios Karamichos, Tor P Utheim, Rakibul Islam, May Griffith, M Mirazul Islam, Robin R Hodges, Gary E Wnek, David L Kaplan, Darlene A Dartt
Purpose: We fabricated and investigated polymeric scaffolds that can substitute for the conjunctival extracellular matrix to provide a substrate for autologous expansion of human conjunctival goblet cells in culture. Methods: We fabricated two hydrogels and two silk films: (1) recombinant human collagen (RHC) hydrogel, (2) recombinant human collagen 2-methacryloylxyethyl phosphorylcholine (RHC-MPC) hydrogel, (3) arginine-glycine-aspartic acid (RGD) modified silk, and (4) poly-D-lysine (PDL) coated silk, and four electrospun scaffolds: (1) collagen, (2) poly(acrylic acid) (PAA), (3) poly(caprolactone) (PCL), and (4) poly(vinyl alcohol) (PVA)...
November 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27828487/joint-tracking-and-equalization-scheme-for-multi-polarization-effects-in-coherent-optical-communication-systems
#15
Yiqiao Feng, Linqian Li, Jiachuan Lin, Hengying Xu, Wenbo Zhang, Xianfeng Tang, Lixia Xi, Xiaoguang Zhang
We propose a joint multi-polarization-effect tracking and equalization method based on two extended Kalman filters, which can cope with state of polarization (SOP) tracing, polarization demultiplexing, equalization for polarization dependent loss (PDL) and polarization mode dispersion (PMD) in PDM-M-QAM coherent optical communication system. The mathematical model of the proposed method is given and analyzed in detail. Through simulation, the proposed method is proved to be very effective in a 28 Gbaud/s PDM-16QAM system...
October 31, 2016: Optics Express
https://www.readbyqxmd.com/read/27828142/design-and-fabrication-of-a-low-loss-and-asymmetric-1%C3%A2-%C3%A3-%C3%A2-5-arbitrary-optical-power-splitter
#16
Liang Liang Wang, Jun Ming An, Jia Shun Zhang, Yuan Da Wu, Jian Guang Li, Xiao Jie Yin, Hong Jie Wang, Yue Wang, Fei Zhong, Qiang Zha, Xiong Wei Hu
In this paper, a low-loss and wavelength-insensitive asymmetric 1×5 arbitrary optical power splitter is designed and optimized, which is composed of a three-branch and two Y-branch elements. A low-loss and wavelength-independent arbitrary 1×5 optical power splitter can be obtained. Asymmetric 1×5 optical power splitters of different output ratios are fabricated using silica-based planar lightwave circuit (PLC) technology on a 6-inch quartz substrate. The measured results show that the IL<sub>output1</sub>, IL<sub>output2∼5</sub>, UNIF<sub>output2∼5</sub>, PDL, and WDL of the output ratio 33%:67%, 50%:50%, and 95%:5% are less than (5...
October 20, 2016: Applied Optics
https://www.readbyqxmd.com/read/27816392/assessing-pdl-1-and-pd-1-in-non-small-cell-lung-cancer-a-novel-immunoscore-approach
#17
Erna-Elise Paulsen, Thomas K Kilvaer, Mehrdad Rakaee Khanehkenari, Samer Al-Saad, Sigurd M Hald, Sigve Andersen, Elin Richardsen, Nora Ness, Lill-Tove Busund, Roy M Bremnes, Tom Donnem
INTRODUCTION: Immune checkpoint inhibitors targeting programmed cell death protein 1 (PD-1) or its ligand, PD-L1, have gained momentum in the treatment of non-small cell lung cancer (NSCLC). However, their prognostic significance remains controversial. The present study evaluated the expression of PD-L1 and PD-1 and their potential role in an Immunoscore, supplementing the TNM classification of NSCLC. MATERIALS AND METHODS: Tissue microarrays constructed from tumor tissue samples from 2 cohorts of a total of 536 patients (University Hospital of North Norway, n = 285; Nordland Hospital, n = 251) with primary resected stage I to IIIA NSCLC...
October 5, 2016: Clinical Lung Cancer
https://www.readbyqxmd.com/read/27812916/gabab-receptors-as-modulating-target-for-inflammatory-responses-of-the-periodontal-ligament
#18
Anna Konermann, Thomas Van Dyke, Alpdogan Kantarci, Andreas Jäger
PDL cells express GABAB1 and GABAB2 receptors, which are regulated by inflammation and thus might be implicated in periodontal immunology. It was the aim of this study to elucidate the functional role of GABAB receptors in immunomodulation regarding activation of proregenerative versus proinflammatory mechanisms. Human PDL cells were exposed to GABA and/or GABAB receptor antagonist CGP-52432 alone or in combination with IL-1β to mimic inflammation. The influence on marker expression for inflammatory tissue destruction was determined via qRT-PCR and Luminex assays...
November 3, 2016: Cellular and Molecular Neurobiology
https://www.readbyqxmd.com/read/27806346/cross-talk-between-microbiota-and-immune-fitness-to-steer-and-control-response-to-anti-pd-1-pdl-1-treatment
#19
REVIEW
Andrea Botticelli, Ilaria Zizzari, Federica Mazzuca, Paolo Antonio Ascierto, Lorenza Putignani, Luca Marchetti, Chiara Napoletano, Marianna Nuti, Paolo Marchetti
Immune Checkpoint Inhibitors (ICIs) are improving the survival of cancer patients, however only the 20-30% of treated patients present clinical benefits. Toxicity represents the major cause of reduced dosage, delayed drug administration and therapy discontinuation. Hence in the context of multiple treatment possibilities, the identification of predictive markers of response and toxicity is a challenging approach for drug selection in order to obtain the best clinical benefit while minimizing the side effects...
October 28, 2016: Oncotarget
https://www.readbyqxmd.com/read/27806071/fosfomycin-addition-to-poly-d-l-lactide-coating-does-not-affect-prophylaxis-efficacy-in-rat-implant-related-infection-model-but-that-of-gentamicin-does
#20
Anil Gulcu, Alp Akman, Ahmet Fahir Demirkan, Ali Cagdas Yorukoglu, Ilknur Kaleli, Ferda Bir
Gentamicin is the preferred antimicrobial agent used in implant coating for the prevention of implant-related infections (IRI). However, the present heavy local and systemic administration of gentamicin can lead to increased resistance, which has made its future use uncertain, together with related preventive technologies. Fosfomycin is an alternative antimicrobial agent that lacks the cross-resistance presented by other classes of antibiotics. We evaluated the efficacy of prophylaxis of 10% fosfomycin-containing poly(D,L-lactide) (PDL) coated K-wires in a rat IRI model and compared it with uncoated (Control 1), PDL-coated (Control 2), and 10% gentamicin-containing PDL-coated groups with a single layer of coating...
2016: PloS One
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