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Lavinia Gambelli, Simon Guerrero-Cruz, Rob J Mesman, Geert Cremers, Mike S M Jetten, Huub J M Op den Camp, Boran Kartal, Claudia Lueke, Laura van Niftrik
Methane is a very potent greenhouse gas and can be oxidized aerobically or anaerobically through microbial-mediated processes, thus decreasing methane emissions to the atmosphere. Using a complementary array of methods including phylogenetic analysis, physiological experiments, and light and electron microscopy techniques (including electron tomography), we investigated the community composition and ultrastructure of a continuous bioreactor enrichment culture, in which anaerobic methane oxidation (AOM) was coupled to nitrate reduction...
November 17, 2017: Applied and Environmental Microbiology
Xiao-Cong Zhou, Chun-Wei Huang, Yan-Yan Dai, Zhi-Yang Huang, Zheng Lou
RATIONALE: Colonoscopy is a relatively safe and common procedure with low risks of complications, and acute appendicitis with perforation is an extremely rare complication of colonoscopy. The current study presents an unusual case of acute gangrenous appendicitis with perforation following a screening colonoscopy. PATIENT CONCERNS: A 73-year-old man presented to our emergency department with lower right abdominal pain 3 days after a routine screening colonoscopy...
November 2017: Medicine (Baltimore)
Yu-Bo Zhou, Hong-Tian Li, Leonardo Trasande, Lin-Lin Wang, Ya-Li Zhang, Ke-Yi Si, Man-Xi Bai, Jian-Meng Liu
Adequate docosahexaenoic acid (DHA) is essential for the optimal growth and development of the fetus. Maternal DHA content fluctuates during pregnancy. The correlation of DHA content with dietary intake might be varied over the course of pregnancy. We aimed to compare the dietary DHA intake, estimated by a DHA-specific semiquantitative food frequency questionnaire (FFQ) against its blood content, among mid- and late-term pregnant women. A total of 804 Chinese pregnant women completed the tailored FFQ and provided fasting venous blood samples...
November 16, 2017: Nutrients
Lang-Hong Wang, Xin-An Zeng, Man-Sheng Wang, Charles S Brennan, Deming Gong
In this work, modifications of cell membrane fluidity, fatty acid composition and fatty acid biosynthesis-associated genes of Escherichia coli ATCC 25922 (E. coli) and Staphylococcus aureus ATCC 6538 (S. aureus), during growth in the presence of naringenin (NAR), one of the natural antibacterial components in citrus plants, was investigated. Compared to Escherichia coli, the growth of Staphylococcus aureus was significantly inhibited by NAR in low concentrations. Combination of gas chromatography-mass spectrometry with fluorescence polarization analysis revealed that E...
November 11, 2017: Biochimica et Biophysica Acta
Sung Man Park, Yu Ran Lee, Do Won Kang, Hong Lae Kim, Chan Ho Kwon
One-photon vacuum ultraviolet mass-analyzed threshold ionization (VUV-MATI) spectroscopy was used to characterize the essential conformations of tetrahydrofuran (THF) and thus determine the stereochemistry of the furanose ring constituting the backbones of DNA and RNA. Since the VUV-MATI spectrum of THF exactly corresponds to the vibrational spectrum of the gas-phase THF cation, the above cation was detected using time-of-flight mass spectrometry featuring the delayed pulsed-field ionization of the target in high Rydberg states by scanning the wavelength of the VUV pulse across the region of the vibrational spectrum...
November 8, 2017: Physical Chemistry Chemical Physics: PCCP
Tatsunori Takayasu, Hiroki Yamamoto, Yuko Ishida, Mizuho Nosaka, Mariko Kawaguchi, Yumi Kuninaka, Akihiko Kimura, Toshikazu Kondo
We herein report a fatal intoxication case caused by the ingestion of the insecticides chlorpyrifos-methyl (CPFM) and fenitrothion (MEP). A 70-year-old man was found dead in his house and a cup containing a small amount of agricultural chemicals was on the table near his body. External and internal examinations revealed no injuries. In a gas chromatography-mass spectrometry (GC-MS) screening test, CPFM, MEP, and their metabolites, 3,5,6-trichloro-2-pyridinol (TCPY) and 3-methyl-4-nitrophenol (3MNP), respectively, were qualitatively detected in his stomach contents...
October 4, 2017: Legal Medicine
Zijun Yan, Man He, Beibei Chen, Bo Gui, Cheng Wang, Bin Hu
Covalent triazine frameworks (CTFs), featuring with high surface area, good thermal, chemical and mechanical stability, are good adsorbents in sample pretreatment. Herein, magnetic CTFs/Ni composite was prepared by in situ reduction of nickel ions on CTFs matrix with a solvothermal method. The prepared CTFs/Ni composite exhibited good preparation reproducibility, high chemical stability, and high extraction efficiency for targeted phthalate esters (PAEs) due to π-π interaction and hydrophobic effect. The porous structure of CTFs/Ni composite benefited the fast transfer of target PAEs from aqueous solution to the adsorbents, and the integrated magnetism contributed to the rapid separation of adsorbents from sample and elution solution...
October 10, 2017: Journal of Chromatography. A
Yan Man, Gang Liang, An Li, Ligang Pan
Mycotoxins are one of the main factors impacting food safety. Mycotoxin contamination has threatened the health of humans and animals. Conventional methods for the detection of mycotoxins are gas chromatography (GC) or liquid chromatography (LC) coupled with mass spectrometry (MS), or enzyme-linked immunosorbent assay (ELISA). However, all these methods are time-consuming, require large-scale instruments and skilled technicians, and consume large amounts of hazardous regents and solvents. Interestingly, a microchip requires less sample consumption and short analysis time, and can realize the integration, miniaturization, and high-throughput detection of the samples...
October 14, 2017: Toxins
Lei-Xiong, Ke-Hui Ouyang, Yan-Jiang, Zhan-Wei Yang, Wen-Bing Hu, Hui-Chen, Ning-Wang, Xin-Liu, Wen-Jun Wang
This study was designed to study the chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage. A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fourier transform infrared spectra (FT-IR), UV-vis, gas chromatography-mass spectrometry (GC-MS), high performance gel chromatography (HPGLC) and scanning electron microscopy (SEM). Cell viability was measured by MTT test, phagocytosis assay was measured by Neutral red uptake assay, nitrite was measured by Griess assay, TNF-α and IL-1β analysis were measured by ELISA, PGE2 was measured by enzyme immunoassay system...
October 12, 2017: International Journal of Biological Macromolecules
Prayuth Tunsuriyawong, Krit Pongpirul, Tagann Chaisam, Petpring Prajuabpansri
An 18-year-old Caucasian man presented with a lack of sense of surrounding smell. The problem was first noticed when a family member discussed the smell of the food, which he had no idea what it was. The patient had normal development and sexual function, no history of trauma, surgery, chemical exposure or infection. Physical examination revealed no significant abnormalities. Smell threshold test using phenyl-ethyl-alcohol revealed bilateral anosmia. MRI showed bilateral aplastic olfactory bulbs and tracts associated with absent cortical growth of the olfactory sulci and asymmetrical gyrus rectus...
October 11, 2017: BMJ Case Reports
Andrea Appeltans, Marco Mura, Giulio Bamonte
INTRODUCTION: The purpose of this report is to describe a case of macular hole development after vitrectomy for floaters with induction of posterior vitreous detachment. CASE REPORT: A 44-year-old otherwise healthy man presented with visually debilitating floaters in his right eye; these had been present for more than 2 years. Preoperative examination was unremarkable in both eyes, apart from some degree of vitreous degeneration in the right eye. Preoperative visual acuity was 20/20 bilaterally...
December 2017: Ophthalmology and Therapy
Takako Sato, Hiroshi Nishioka, Kento Tsuboi, Munehiro Katagi, Akihiro Miki, Takashi Saito, Shuntaro Abe, Masakatsu Nomura, Misa Kitagawa, Hitoshi Tsuchihashi, Koichi Suzuki
In Japan, there are increasing reports of death by poisoning following butane abuse. To determine the specific cause of death in such cases, it is important to confirm the presence of fuel gas components in the body, although careful analysis is required because of their volatile properties. In most reported cases, the subject died suddenly during or immediately after butane aspiration. Thus, the butane concentration in the samples from the deceased should be relatively high. Herein, we present a case of an 18-year-old man found with cardiopulmonary arrest, who then exhibited hypoxic encephalopathy for 16days in a hospital...
October 3, 2017: Legal Medicine
Rachel J Perry, Liang Peng, Gary W Cline, Gina M Butrico, Yongliang Wang, Xian-Man Zhang, Douglas L Rothman, Kitt Falk Petersen, Gerald I Shulman
Hepatic mitochondria play a central role in the regulation of intermediary metabolism and maintenance of normoglycemia, and there is great interest in assessing rates of hepatic mitochondrial citrate synthase flux (V CS) and pyruvate carboxylase flux (V PC) in vivo. Here, we show that a positional isotopomer NMR tracer analysis (PINTA) method can be used to non-invasively assess rates of V CS and V PC fluxes using a combined NMR/gas chromatography-mass spectrometry analysis of plasma following infusion of [3-(13)C]lactate and glucose tracer...
October 6, 2017: Nature Communications
Prashant N Mohite, Alexander Rosenberg, Clara Hernández Caballero, Simona Soresi, Javid Fatullayev, Anna Reed, Aron-Frederik Popov, Anton Sabashnikov, André R Simon
Extracorporeal membrane oxygenation (ECMO) as a bridge to lung transplant (LTx) is not uncommon, but it is not commonplace yet. We present a case of a 45-year-old man with cystic fibrosis with recent deterioration in lung function who was initially supported with veno-venous (VV) ECMO. However, he subsequently required conversion to veno-veno-arterial (VVA) ECMO. After 21 days of support, he underwent double lung transplantation, with an uneventful postoperative course. This case shows that, in patients with end-stage respiratory failure awaiting lung transplantation, extracorporeal life support may require escalation to improve gas exchange and address circulatory requirements...
October 2017: Perfusion
Ho-Kyung Lim, Soo-Hwan Byun, Jae-Man Woo, Sae-Mi Kim, Sung-Mi Lee, Bong-Ju Kim, Hyoun-Ee Kim, Jung-Woo Lee, Soung-Min Kim, Jong-Ho Lee
Magnesium (Mg) has the advantage of being resorbed in vivo, but its resorption rate is difficult to control. With uncontrolled resorption, Magnesium as a bone fixation material has minimal clinical value. During resorption not only is the strength rapidly weakened, but rapid formation of metabolite also occurs. In order to overcome these disadvantages, hydroxyapatite (HA) surface coating of pure magnesium plate was attempted in this study. Magnesium plates were inserted above the frontal bone of Sprague-Dawley rats in both the control group (Bare-Mg group) and the experimental group (HA-Mg group)...
September 30, 2017: Materials
Alisha Z Jiwani, Vikhyat S Bebarta, Leopoldo C Cancio
CONTEXT: Hydroxocobalamin is an effective cyanide antidote. While erythema, hypertension, and chromaturia are recognized side effects, methemoglobinemia has not been reported. Methemoglobin levels are most accurately measured by co-oximetry. We describe an extensively burned patient who developed methemoglobinemia within an hour of hydroxocobalamin administration. CASE DETAILS: A 47-year old man without genetic deficiencies or abnormal hemoglobin variants presented with 61% body surface area thermal burns and grade 1 inhalation injury sustained during a tugboat engine explosion...
October 3, 2017: Clinical Toxicology
Seunggun Yu, Hyesung Cho, Jun Pyo Hong, Hyunchul Park, Jason Christopher Jolly, Hong Suk Kang, Jin Hong Lee, Junsoo Kim, Seung Hwan Lee, Albert S Lee, Soon Man Hong, Cheolmin Park, Shu Yang, Chong Min Koo
Designing topographic clusters is of significant interest, yet it remains challenging as they often lack mobility or deformability. Here we exploit the huge volumetric expansion (up to 3000%) of a new type of building block, thermally expandable microbombs. They consist of a viscoelastic polymeric shell and a volatile gas core, which, within structural confinement, create micro-clusters via inverse jamming and topographical close-packing. Upon heating, microbombs anchored in rigid confinement underwent balloon-like blowing up, allowing for dense clusters via soft interplay between viscoelastic shells...
September 28, 2017: Nature Communications
Weixiao Dou, Zhen Zhou, Jiongjiong Ye, Rongwei Huang, Lu-Man Jiang, Guofeng Chen, Xiaoyun Fei
Flue gas desulfurization (FGD) wastewater treatment by conventional neutralization, chemical precipitation and coagulation process removes most suspended solids and heavy metals, and provides an effluent rich in calcium, alkalinity and chloride, which obstructs its reclamation and reuse but is in favor of phosphorus (P) precipitation. The goals of this study were to investigate feasibility of reusing FGD effluent as a calcium source for P removal from P-rich wastewater. Results revealed that increasing the volumetric ratio between FGD effluent and P-rich wastewater achieved higher pH value and Ca/P ratio, and thus enhanced P removal efficiency to 94...
September 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
A Alperen Günay, Soumyadip Sett, Junho Oh, Nenad Miljkovic
Droplet evaporation is an important phenomenon governing many man-made and natural processes. Characterizing the rate of evaporation with high accuracy has attracted the attention of numerous scientists over the past century. Traditionally, researchers have studied evaporation by observing the change in the droplet size in a given time interval. However, the transient nature coupled with the significant mass-transfer-governed gas dynamics occurring at the droplet three-phase contact line makes the classical method crude...
October 10, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
M Queißer, M R Burton, F Arzilli, A Chiarugi, G I Marliyani, F Anggara, A Harijoko
Studying the quantity and origin of CO2 emitted by back-arc mud volcanoes is critical to correctly model fluid-dynamical, thermodynamical, and geochemical processes that drive their activity and to constrain their role in the global geochemical carbon cycle. We measured CO2 fluxes of the Bledug Kuwu mud volcano on the Kendeng Fold and thrust belt in the back arc of Central Java, Indonesia, using scanning remote sensing absorption spectroscopy. The data show that the expelled gas is rich in CO2 with a volume fraction of at least 16 vol %...
June 2017: Journal of Geophysical Research. Solid Earth
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