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https://www.readbyqxmd.com/read/28449327/skeletal-muscle-il-15-il-15r%C3%AE-and-myofibrillar-protein-synthesis-after-resistance-exercise
#1
Alberto Pérez-López, James McKendry, Marcos Martin-Rincon, David Morales-Alamo, Bárbara Pérez-Köhler, David Valadés, Julia Buján, Jose A L Calbet, Leigh Breen
In vitro and in vivo studies described the myokine IL-15 and its receptor IL-15Rα as anabolic/anti-atrophy agents, however the protein expression of IL-15Rα has not been measured in human skeletal muscle and data regarding IL-15 expression remain inconclusive. The purpose of the study was to determine serum and skeletal muscle IL-15 and IL-15Rα responses to resistance exercise session and to analyse their association with myofibrillar protein synthesis (MPS). Fourteen participants performed a bilateral leg resistance exercise composed of 4 sets of leg press and 4 sets of knee extension at 75% 1RM to task failure...
April 27, 2017: Scandinavian Journal of Medicine & Science in Sports
https://www.readbyqxmd.com/read/28447690/bacterial-magnetosomes-nature-s-powerful-contribution-to-mpi-tracer-research
#2
A Kraupner, D Eberbeck, D Heinke, R Uebe, D Schüler, A Briel
The magnetic particle spectrum (MPS) of bacterial magnetosomes, isolated from Magnetospirillum gryphiswaldense, is measured and compared to that of the current "gold standard", Resovist®. It is shown that the amplitudes of the magnetosomes' harmonics by far exceed that of Resovist®; the amplitude of the third harmonic is higher by a factor of 7, and is the highest value obtained for iron oxide nanoparticles to date.
April 27, 2017: Nanoscale
https://www.readbyqxmd.com/read/28443964/effects-of-chronic-exercise-on-endothelial-progenitor-cells-and-microparticles-in-professional-runners
#3
Célia Regina de Oliveira Bittencourt, Maria Cristina de Oliveira Izar, Carolina Nunes França, Valdir Lauro Schwerz, Rui Manuel Dos Santos Póvoa, Francisco Antonio Helfenstein Fonseca
BACKGROUND: The effects of chronic exposure to exercise training on vascular biomarkers have been poorly explored. OBJECTIVE: Our study aimed to compare the amounts of endothelial progenitor cells (EPCs), and endothelial (EMP) and platelet (PMP) microparticles between professional runners and healthy controls. METHODS: Twenty-five half-marathon runners and 24 age- and gender-matched healthy controls were included in the study. EPCs (CD34+/KDR+, CD133+/KDR+, and CD34+/CD133+), EMP (CD51+) and PMP (CD42+/CD31+) were quantified by flow-cytometry...
March 2017: Arquivos Brasileiros de Cardiologia
https://www.readbyqxmd.com/read/28442543/effect-of-informal-employment-on-the-relationship-between-psychosocial-work-risk-factors-and-musculoskeletal-pain-in-central-american-workers
#4
David Gimeno Ruiz de Porras, Marianela Rojas Garbanzo, Aurora Aragón, Lino Carmenate-Milián, Fernando G Benavides
INTRODUCTION: The constant increase on the psychosocial demands experienced at work seems to contribute to the increase in health problems such as musculoskeletal pain (MSP). This association may be especially important in low-income and middle-income countries, where there is a large proportion of informal workers among whom there is little research. We analysed the association between psychosocial work risk factors and MSP among formal and informal workers using the First Central American Survey of Working Conditions and Health...
April 25, 2017: Occupational and Environmental Medicine
https://www.readbyqxmd.com/read/28440931/microplastics-as-vectors-for-environmental-contaminants-exploring-sorption-desorption-and-transfer-to-biota
#5
Nanna B Hartmann, Sinja Rist, Julia Bodin, Louise Hs Jensen, Stine N Schmidt, Philipp Mayer, Anders Meibom, Anders Baun
The occurrence and effects of microplastics (MPs) in the aquatic environment are receiving increasing attention. In addition to their possible direct adverse effects on biota, the potential role of MPs as vectors for hydrophobic organic chemicals (HOCs), compared to natural pathways, is a topic of much debate. It is evident, however, that temporal and spatial variations of MP occurrence do (and will) occur. To further improve the estimations of the role of MPs as vectors for HOC transfer into biota under varying MP concentrations and environmental conditions, it is important to identify and understand the governing processes...
May 2017: Integrated Environmental Assessment and Management
https://www.readbyqxmd.com/read/28437975/experimental-demonstration-of-efficient-quantum-state-tomography-of-matrix-product-states
#6
Yuan-Yuan Zhao, Zhibo Hou, Guo-Yong Xiang, Yong-Jian Han, Chuan-Feng Li, Guang-Can Guo
Quantum state tomography is a key technology for fully determining a quantum state. Unfortunately, standard quantum state tomography is intractable for general many-body quantum states, because the number of measurements and the post-processing time increase exponentially with the size of the system. However, for the matrix product states (MPSs), there exists an efficient method using linearly scaled local measurements and polynomially scaled post-processing times. In this study, we demonstrate the validity of the method in practice by reconstructing a four-photon MPS from its local two- or three-photon reduced-density matrices with the presence of statistical errors and systematical errors in experiment...
April 17, 2017: Optics Express
https://www.readbyqxmd.com/read/28436478/tissue-accumulation-of-microplastics-in-mice-and-biomarker-responses-suggest-widespread-health-risks-of-exposure
#7
Yongfeng Deng, Yan Zhang, Bernardo Lemos, Hongqiang Ren
Microplastics (MPs) are a significant environmental health issue and increasingly greater source of concern. MPs have been detected in oceans, rivers, sediments, sewages, soil and even table salts. MPs exposure on marine organisms and humans has been documented, but information about the toxicity of MPs in mammal is limited. Here we used fluorescent and pristine polystyrene microplastics (PS-MPs) particles with two diameters (5 μm and 20 μm) to investigate the tissue distribution, accumulation, and tissue-specific health risk of MPs in mice...
April 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28432840/detection-of-egfr-mutations-in-patients-with-non-small-cell-lung-cancer-by-high-resolution-melting-comparison-with-other-methods
#8
Carlos Martínez-Carretero, Fernando Iguaz Pascual, Antonio Rus, Ivan Bernardo
BACKGROUND: The discovery of mutations in the epidermal growth factor receptor gene (EGFR) related to the clinical response to tyrosine kinase inhibitors, has transformed the management of non-small cell lung cancer (NSCLC). Several methods have been developed for determination of mutations in EGFR, with different sensitivity and potential ability to detect a different number of mutations. METHODS: We developed a screening method by high resolution melting (HRM) to detect EGFR mutations, and compared the results of 123 fixed in formalin and paraffin embedded (FFPE) tumor tissue samples with the detection of mutations by allele-specific PCR...
April 22, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28429088/an-snp-panel-for-the-analysis-of-paternally-inherited-alleles-in-maternal-plasma-using-ion-torrent-pgm
#9
Donggui Yang, Hao Liang, Shaobin Lin, Qing Li, Xiaoyan Ma, Jun Gao, Hongyu Sun, Qingqing Chen, Jianzhu Wu, Xueling Ou
Researchers have sought to develop an effective protocol for paternity analysis using cell-free DNA (cfDNA) in maternal plasma. The use of massively parallel sequencing (MPS) technology for SNP testing is attractive because of its high-throughput capacity and resolution to single-base precision. In this study, we designed a customized SNP panel for cfDNA sequencing that includes 720 short amplicons (< 140 bp) targeting SNPs on the autosome and Y chromosome. The systemic performance was evaluated using the Ion Torrent PGM, indicating balanced coverage among most of the included loci, except for 78 poorly performing SNPs that were observed to have an inconsistent allele balance, lower coverage reads or high background signals...
April 20, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28428471/motion-frozen-imaging-by-gated-myocardial-perfusion-single-photon-emission-computed-tomography-using-multi-focus-fan-beam-collimator-in-thallium-201-study
#10
Kaito Takahashi, Takashi Takeuchi, Yoshiyuki Hosokai, Hayato Odagiri, Haruo Saito
PURPOSE: This study aimed to evaluate the statistical noise of motion-frozen (MF) image generated by gated myocardial perfusion single photon emission computed tomography (SPECT) imaging using IQ · SPECT and to determine the optimal acquisition and reconstruction parameters for MF image using IQ · SPECT. METHODS: A movement cardiac phantom and static cardiac phantom were used to acquire the MF images. The acquisition times used were different in 8 and 16 frames per R-R interval, and varying reconstruction parameters (subset and iteration) were used...
2017: Nihon Hoshasen Gijutsu Gakkai Zasshi
https://www.readbyqxmd.com/read/28428354/multiplex-tandem-mass-spectrometry-enzymatic-activity-assay-for-newborn-screening-of-the-mucopolysaccharidoses-and-type-2-neuronal-ceroid-lipofuscinosis
#11
Yang Liu, Fan Yi, Arun Babu Kumar, Naveen Kumar Chennamaneni, Xinying Hong, C Ronald Scott, Michael H Gelb, Frantisek Turecek
BACKGROUND: We expanded the use of tandem mass spectrometry combined with liquid chromatography (LC-MS/MS) for multiplex newborn screening of seven lysosomal enzymes in dried blood spots (DBS). The new assays are for enzymes responsible for the mucopolysaccharidoses (MPS-I, -II, -IIIB, -IVA, -VI, and -VII) and type 2 neuronal ceroid lipofuscinosis (LINCL). METHODS: New substrates were prepared and char-acterized for tripeptidyl peptidase 1 (TPP1), α-N-acetylglucosaminidase (NAGLU), and lysosomal β-glucuronidase (GUSB)...
April 20, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28427773/bioturbation-transports-secondary-microplastics-to-deeper-layers-in-soft-marine-sediments-of-the-northern-baltic-sea
#12
Pinja Näkki, Outi Setälä, Maiju Lehtiniemi
Microplastics (MPs) are observed to be present on the seafloor ranging from coastal areas to deep seas. Because bioturbation alters the distribution of natural particles on inhabited soft bottoms, a mesocosm experiment with common benthic invertebrates was conducted to study their effect on the distribution of secondary MPs (different-sized pieces of fishing line<1mm). During the study period of three weeks, the benthic community increased MP concentration in the depth of 1.7-5.1cm in the sediment. The experiment revealed a clear vertical gradient in MP distribution with their abundance being highest in the uppermost parts of the sediment and decreasing with depth...
April 17, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28426871/macular-pigment-density-and-quantitative-fundus-autofluorescence-in-young-healthy-subjects
#13
Chiara M Eandi, Marco Nassisi, Carlo Lavia, Camilla Alovisi, Ugo de Sanctis
Purpose: To measure macular pigment (MP) and find possible correlation between heterochromatic flicker photometry (HFP) and quantitative autofluorescence (qAF) in young healthy subjects. Methods: We enrolled 80 eyes of 40 young healthy subjects. Macular pigment optical density (MPOD) was automatically calculated with a macular pigment screener (MPS; MPODHFP). We calculated qAF comparing gray levels (GL) of qAF images with GL of internal reference of a confocal scanning laser ophthalmoscopy...
April 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28425064/a-pathway-to-personalizing-therapy-for-metastases-using-liver-on-a-chip-platforms
#14
A S Khazali, A M Clark, A Wells
Metastasis accounts for most cancer-related deaths. The majority of solid cancers, including those of the breast, colorectum, prostate and skin, metastasize at significant levels to the liver due to its hemodynamic as well as tumor permissive microenvironmental properties. As this occurs prior to detection and treatment of the primary tumor, we need to target liver metastases to improve patients' outcomes. Animal models, while proven to be useful in mechanistic studies, do not represent the heterogeneity of human population especially in drug metabolism lack proper human cell-cell interactions, and this gap between animals and humans results in costly and inefficient drug discovery...
April 19, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/28424820/microparticles-during-long-term-follow-up-after-acute-myocardial-infarction-association-to-atherosclerotic-burden-and-risk-of-cardiovascular-events
#15
Christina Christersson, Åsa Thulin, Agneta Siegbahn
Microparticles (MPs) are formed from platelets (PMPs), endothelial cells (EMPs) and monocytes (MMPs), and in acute myocardial infarction (MI), there is an increase of MPs in the culprit artery. In this study MPs were evaluated in whole blood in 105 patients with MI at five time-points during a two-year follow-up (FU). Patients with non-ST-elevated MI had higher concentrations of CD41+MPs compared to ST-elevated MI patients (p=0.024). The concentrations of PMPs in whole blood increased during the time period (p<0...
April 20, 2017: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/28423578/endothelial-microparticles-delivering-microrna-155-into-t-lymphocytes-are-involved-in-the-initiation-of-acute-graft-versus-host-disease-following-allogeneic-hematopoietic-stem-cell-transplantation
#16
Ran Zhang, Xiaoxiao Wang, Mei Hong, Ting Luo, Miaomiao Zhao, Haorui Shen, Jun Fang, Xiaojie Li, Sibin Zang, Ping Chen, Dimin Nie, Peng Zheng, Qiuling Wu, Linghui Xia
Endothelial microparticles (EMPs) upregulation has been observed in acute graft-versus-host disease (aGVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT). However, the role of EMPs remains unclear. We found that EMPs derived from TNF-α-stimulated human umbilical vein endothelial cells (EA.hy926) concentrated more microRNA-155 (miR-155) compared with maternal cells. The miR-155 levels in MPs from peripheral blood of aGVHD patients and mice were remarkably elevated and significantly higher than the levels in plasma...
April 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/28423163/metafrontier-frameworks-for-studying-hospital-productivity-growth-and-quality-changes
#17
Kuan-Chen Chen, Li-Nien Chien, Yi-Hsin Hsu, Ming-Miin Yu
Objective: The objective of this study was to evaluate productivity growth and quality changes among different levels of accredited hospitals. Design: This study used an attribute-incorporating Malmquist productivity index (MPI) under the metafrontier framework. This is the first attempt to compare productivity changes among hospitals operating under different production frontiers. Participants: The data consisted of 20 academic medical centers (AMCs), 61 metropolitan hospitals (MPs) and 112 local community hospitals (LCs) in Taiwan during the period 2007-2010...
December 1, 2016: International Journal for Quality in Health Care
https://www.readbyqxmd.com/read/28418018/neurodevelopmental-changes-in-excitatory-synaptic-structure-and-function-in-the-cerebral-cortex-of-sanfilippo-syndrome-iiia-mice
#18
Chrissa A Dwyer, Samantha L Scudder, Ying Lin, Lara E Dozier, Dustin Phan, Nicola J Allen, Gentry N Patrick, Jeffrey D Esko
Sanfilippo syndrome, MPS IIIA-D, results from deficits in lysosomal enzymes that specifically degrade heparan sulfate, a sulfated glycosaminoglycan. The accumulation of heparan sulfate results in neurological symptoms, culminating in extensive neurodegeneration and early death. To study the impact of storage in postnatal neurodevelopment, we examined murine models of MPS IIIA, which lack the enzyme sulfamidase. We show that changes occur in excitatory postsynaptic structure and function in the somatosensory cortex prior to signs of neurodegeneration...
April 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28417259/comparison-between-magnetic-bead-and-qpcr-library-normalisation-methods-for-forensic-mps-genotyping
#19
Bhavik Mehta, Samantha Venables, Paul Roffey
Massively parallel sequencing (MPS) is fast approaching operational use in forensic science, with the capability to analyse hundreds of DNA identity and DNA intelligence markers in multiple samples simultaneously. The ForenSeq™ DNA Signature Kit on MiSeq FGx™ (Illumina) workflow can provide profiles for autosomal short tandem repeats (STRs), X chromosome and Y chromosome STRs, identity single nucleotide polymorphisms (SNPs), biogeographical ancestry SNPs and phenotype (eye and hair colour) SNPs from a sample...
April 18, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28415504/in-vitro-evaluation-of-hydroxyapatite-coatings-with-002-crystallographic-texture-deposited-by-micro-plasma-spraying
#20
Yiming Wang, Xiaomei Liu, Tingting Fan, Zhen Tan, Zheng Zhou, Dingyong He
Hydroxyapatite (HA) coatings are usually deposited on the metallic implant to increase the biocompatibility and protect the bloodstream from harmful metal ions. Atmospheric plasma spray (APS) is known as a cost effective deposition method. However, the low crystallinity of APS deposited coating accelerates its dissolution in body fluid. We used micro-plasma spray (MPS) to develop chemically stable HA coatings, and performed APS as reference. Results showed that MPS deposited coatings exhibited (002) crystallographic texture while the reference sample did not...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
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