keyword
MENU ▼
Read by QxMD icon Read
search

FTA

keyword
https://www.readbyqxmd.com/read/29098492/molecular-epidemiology-of-trypanosomatids-and-trypanosoma-cruzi-in-primates-from-peru
#1
Esar Aysanoa, Pedro Mayor, A Patricia Mendoza, Carlos M Zariquiey, E Angelo Morales, Jocelyn G Pérez, Mark Bowler, Julio A Ventocilla, Carlos González, G Christian Baldeviano, Andrés G Lescano
We determined the prevalence rate and risk of infection of Trypanosoma cruzi and other trypanosomatids in Peruvian non-human primates (NHPs) in the wild (n = 126) and in different captive conditions (n = 183). Blood samples were collected on filter paper, FTA cards, or EDTA tubes and tested using a nested PCR protocol targeting the 24Sα rRNA gene. Main risk factors associated with trypanosomatid and T. cruzi infection were genus and the human-animal context (wild vs captive animals). Wild NHPs had higher prevalence of both trypanosomatids (64...
November 2, 2017: EcoHealth
https://www.readbyqxmd.com/read/29078807/relationship-between-trypanosoma-brucei-rhodesiense-genetic-diversity-and-clinical-spectrum-among-sleeping-sickness-patients-in-uganda
#2
Charles D Kato, Claire M Mugasa, Ann Nanteza, Enock Matovu, Vincent P Alibu
OBJECTIVE: Human African trypanosomiasis (HAT) due to Trypanosoma brucei rhodesiense in East and southern Africa is reported to be clinically diverse. We tested the hypothesis that this clinical diversity is associated with a variation in trypanosome genotypes. RESULTS: Trypanosome DNA isolated from HAT patients was genotyped using 7 microsatellite markers directly from blood spotted FTA cards following a whole genome amplification. All markers were polymorphic and identified 17 multi-locus genotypes with 56% of the isolates having replicate genotypes...
October 27, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/29078072/development-of-a-lane-change-risk-index-using-vehicle-trajectory-data
#3
Hyunjin Park, Cheol Oh, Jaepil Moon, Seongho Kim
Surrogate safety measures (SSMs) have been widely used to evaluate crash potential, which is fundamental for the development of effective safety countermeasures. Unlike existing SSMs, which are mainly focused on the evaluation of longitudinal vehicle maneuvering leading to rear-end crashes, this study proposes a new method for estimating crash risk while a subject vehicle changes lanes, referred to as the lane change risk index (LCRI). A novel feature of the proposed methodology is its incorporation of the amount of exposure time to potential crash and the expected crash severity level by applying a fault tree analysis (FTA) to the evaluation framework...
October 24, 2017: Accident; Analysis and Prevention
https://www.readbyqxmd.com/read/29075860/dna-mismatch-repair-deficiency-promotes-genomic-instability-in-a-subset-of-papillary-thyroid-cancers
#4
Mahsa Javid, Thanyawat Sasanakietkul, Norman G Nicolson, Courtney E Gibson, Glenda G Callender, Reju Korah, Tobias Carling
BACKGROUND: Efficient DNA damage repair by MutL-homolog DNA mismatch repair (MMR) enzymes, MLH1, MLH3, PMS1 and PMS2, are required to maintain thyrocyte genomic integrity. We hypothesized that persistent oxidative stress and consequent transcriptional dysregulation observed in thyroid follicles will lead to MMR deficiency and potentiate papillary thyroid tumorigenesis. METHODS: MMR gene expression was analyzed by targeted microarray in 18 papillary thyroid cancer (PTC), 9 paracarcinoma normal thyroid (PCNT) and 10 normal thyroid (NT) samples...
October 11, 2017: World Journal of Surgery
https://www.readbyqxmd.com/read/29056180/using-fault-trees-to-advance-understanding-of-diagnostic-errors
#5
Deevakar Rogith, M Sriram Iyengar, Hardeep Singh
PROBLEM DEFINITION: Diagnostic errors annually affect at least 5% of adults in the outpatient setting in the United States. Formal analytic techniques are only infrequently used to understand them, in part because of the complexity of diagnostic processes and clinical work flows involved. In this article, diagnostic errors were modeled using fault tree analysis (FTA), a form of root cause analysis that has been successfully used in other high-complexity, high-risk contexts. How factors contributing to diagnostic errors can be systematically modeled by FTA to inform error understanding and error prevention is demonstrated...
November 2017: Joint Commission Journal on Quality and Patient Safety
https://www.readbyqxmd.com/read/29046410/correlation-of-treponemal-immunoassay-signal-strength-values-with-reactivity-of-confirmatory-treponemal-testing
#6
Yetunde F Fakile, Heather Jost, Karen W Hoover, Kathleen J Gustafson, Susan M Novak-Weekley, Jeff M Schapiro, Anthony Tran, Joan M Chow, Ina U Park
Automated treponemal immunoassays are used for syphilis screening with the reverse sequence algorithm; discordant results (e.g., enzyme immunoassay [EIA]-reactive, reactive plasma reagin [RPR]-non-reactive) are resolved with a second treponemal test. We conducted a study to determine automated immunoassay signal strength values consistently correlating with reactive confirmatory treponemal testing.We conducted a cross-sectional analysis of four automated immunoassays: BioPlex 2200 microbead immunoassay (MBIA), LIAISON chemiluminesence immunoassay (CIA), ADVIA-Centaur CIA, and TrepSure EIA, and three manual assays: Treponema Pallidum Particle Agglutination (TP-PA), Fluorescent Treponemal Antibody-Absorption (FTA-ABS) test, INNO-LIA line immunoassay...
October 18, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29046324/etv6-ntrk3-and-strn-alk-kinases-fusions-are-recurrent-events-in-papillary-thyroid-cancer-of-adult-population
#7
André U Bastos, Ana Carolina de Jesus, Janete Maria Cerutti
OBJECTIVE: PTC-specific analysis identified novel fusions involving RET, BRAF, NTRK1, NTRK3, AGK and ALK genes in adults and pediatric PTC. Although many novel fusions are PTC-specific event and, therefore, are ideal for diagnosis purposes, validation across additional and larger cohorts is essential for introducing these potential diagnostic or prognostic biomarkers into the clinical practice. As most of the BRAF, NTRK3 and ALK fusions were initially found in pediatric PTC or in more aggressive thyroid carcinomas, and there is a great disparity across population, in this study, we screened a large set of adult sporadic PTC cases for the most prevalent kinases fusion lately described in the TCGA...
October 18, 2017: European Journal of Endocrinology
https://www.readbyqxmd.com/read/29017583/mapping-freshwater-snails-in-north-western-angola-distribution-identity-and-molecular-diversity-of-medically-important-taxa
#8
Fiona Allan, Jose Carlos Sousa-Figueiredo, Aidan M Emery, Rossely Paulo, Clara Mirante, Alfredo Sebastião, Miguel Brito, David Rollinson
BACKGROUND: This study was designed to determine the distribution and identity of potential intermediate snail hosts of Schistosoma spp. in Bengo, Luanda, Kwanza Norte and Malanje Provinces in north-western Angola. This is an area where infection with Schistosoma haematobium, causing urogenital schistosomiasis, is common but little is yet known about transmission of the disease. Angola has had a varied past with regard to disease control and is revitalising efforts to combat neglected tropical diseases...
October 10, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28960364/rapid-and-simple-method-by-combining-fta%C3%A2-card-dna-extraction-with-two-set-multiplex-pcr-for-simultaneous-detection-of-non-o157-shiga-toxin-producing-escherichia-coli-strains-and-virulence-genes-in-food-samples
#9
S A Kim, S H Park, S I Lee, S C Ricke
The aim of this research was to optimize two multiplex polymerase chain reaction (PCR) assays that could simultaneously detect six non-O157 Shiga toxin-producing Escherichia coli (STEC) as well as the three virulence genes. We also investigated the potential of combining the FTA™ card-based DNA extraction with the multiplex PCR assays. Two multiplex PCR assays were optimized using six primer pairs for each non-O157 STEC serogroup and three primer pairs for virulence genes respectively. Each STEC strain specific primer pair only amplified 155, 238, 321, 438, 587 and 750 bp product for O26, O45, O103, O111, O121 and O145 respectively...
September 27, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28939507/toxicological-profile-and-acetylcholinesterase-inhibitory-potential-of-palicourea-deflexa-a-source-of-%C3%AE-carboline-alkaloids
#10
Pablo Ricardo Bertelli, Renata Biegelmeyer, Eduardo Pacheco Rico, Luiz Carlos Klein-Junior, Natally S B Toson, Luciane Minetto, Sergio A L Bordignon, André L Gasper, Sidnei Moura, Diogo L de Oliveira, Amélia T Henriques
Palicourea genus is chemically and taxonomically close to Psychotria genus, a well-known source of neuroactive alkaloids. It has been previously reported the pharmacological potential of these alkaloids in some targets related to the neurodegenerative process. In this context, this study was carried out in order to evaluate the toxic effects and acetylcholinesterase (AChE) inhibitory potential of Palicourea deflexa fraction of total alkaloids (FTA). P. deflexa FTA was analyzed by means of HPLC-DAD and HRMS-ESI...
September 20, 2017: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/28920803/assessment-of-a-lancet-and-swab-blood-sampling-technique-for-surveillance-of-elephant-endotheliotropic-herpesvirus-infection
#11
Javier Lopez, L do Vet M Sc, Jonathan Haycock, Andrew Mckenzie, Katharina Seilern-Moy, Akbar Dastjerdi
Lancing a finger elicits minimal pain in humans and is applied routinely to obtain small volumes of blood for clinical diagnostics. A modified lancet bleeding method and several blood sampling matrices were evaluated in this study for the purpose of routine elephant endotheliotropic herpesvirus (EEHV) surveillance in Asian elephants (Elephas maximus). The procedure enabled weekly sampling from elephants as young as 9 mo of age. The blood sampling matrices were evaluated for their sensitivity measuring β-actin, tumor necrosis factor α, and/or EEHV-1 by quantitative polymerase chain reaction assays...
September 2017: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
https://www.readbyqxmd.com/read/28846585/plutonium-bioassay-testing-of-united-states-atmospheric-nuclear-test-participants-and-occupation-forces-of-hiroshima-and-nagasaki-japan
#12
Dennis Michael Schaeffer, David R Case, Joanna T Ingraham, Paul K Blake
The pilot urine plutonium bioassay testing program engaged 100 atomic veterans and 58 persons representative of the United States general population using the fission track analysis technique, developed by Brookhaven National Laboratory. The methodology, collected data, scientific challenges, and test results are presented herein. The Defense Threat Reduction Agency and its predecessor agencies conducted this program in the late 1990s to the mid-2000s to determine the feasibility of bioassay testing to supplement dose reconstruction to estimate atomic veterans' doses under the agency's Nuclear Test Personnel Review Program...
October 2017: Health Physics
https://www.readbyqxmd.com/read/28841576/diagnostic-value-of-perinatal-autopsies-analysis-of-486-cases
#13
Nalan Neşe, Yeşim Bülbül
AIM: Autopsy is a beneficial procedure to determine the cause of death and the frequency of anomalies in perinatal losses. Even in the event of an autopsy not providing any additional information, completion of the procedure confirming the clinical diagnoses gives reassurance to both clinicians and parents. Here we present a 15-year archival study based on findings of perinatal autopsies. DESIGN AND METHODS: Four hundred and eighty-six cases from our archive were reviewed and according to the findings they were divided into three subcategories; (1) miscarriages (MCF); (2) fetuses terminated (FTA) for vital anomalies detected by prenatal ultrasonography; (3) premature or term newborns died within first month of life (neonates: NN)...
August 25, 2017: Journal of Perinatal Medicine
https://www.readbyqxmd.com/read/28830272/bone-morphological-factors-influencing-cartilage-degeneration-in-the-knee
#14
Kenji Takahashi, Sanshiro Hashimoto, Shinji Kiuchi, Atsuya Watanabe, Hiroshi Nakamura, Futoshi Ikuta, Tomoyuki Okuaki, Tatsunori Kataoka, Tokifumi Majima, Shinro Takai
OBJECTIVES: Morphological features of the distal femur and proximal tibia associated with cartilage degeneration are unknown. This study aimed to elucidate local anatomical parameters of the knee which correlate with articular cartilage degeneration using MRI T1ρ mapping. METHODS: This study involved 200 subjects with knee pain (mean age, 48.7 years; range, 14-80 years) with no severe osteoarthritic changes on plain X-ray. T1ρ values were measured in the regions of interest on the surface layer of the cartilage on mid-coronal images of the femorotibial joint...
August 23, 2017: Modern Rheumatology
https://www.readbyqxmd.com/read/28827007/failure-modes-and-effects-analysis-for-ocular-brachytherapy
#15
Yongsook C Lee, Yongbok Kim, Jason Wei-Yeong Huynh, Russell J Hamilton
PURPOSE: The aim of the study was to identify potential failure modes (FMs) having a high risk and to improve our current quality management (QM) program in Collaborative Ocular Melanoma Study (COMS) ocular brachytherapy by undertaking a failure modes and effects analysis (FMEA) and a fault tree analysis (FTA). METHODS AND MATERIALS: Process mapping and FMEA were performed for COMS ocular brachytherapy. For all FMs identified in FMEA, risk priority numbers (RPNs) were determined by assigning and multiplying occurrence, severity, and lack of detectability values, each ranging from 1 to 10...
August 18, 2017: Brachytherapy
https://www.readbyqxmd.com/read/28820347/association-between-eye-diagnosis-and-positive-syphilis-test-results-in-a-large-urban-sexually-transmitted-infection-primary-care-clinic-population
#16
Ann-Marie Lobo, Yan Gao, Laura Rusie, Magda Houlberg, Supriya D Mehta
In 2015, the Centers for Disease Control and Prevention (CDC) and the American Academy of Ophthalmology (AAO) released clinical advisories on rising cases of ocular syphilis. We examined the association between eye disease and syphilis infection among primary care and sexually transmitted infection (STI) clinic patients attending an urban lesbian, gay, bisexual, transgender (LGBT) health center. We conducted a retrospective medical record review of all patients who underwent syphilis testing at Howard Brown Health between 1 January 2010 and 31 December 2015...
January 1, 2017: International Journal of STD & AIDS
https://www.readbyqxmd.com/read/28812041/anthropometric-and-skeletal-parameters-predict-2-strand-semitendinosus-tendon-size-in-double-bundle-anterior-cruciate-ligament-reconstruction
#17
Daisuke Chiba, Eiichi Tsuda, Shizuka Sasaki, Xizhe Liu, Yasuyuki Ishibashi
BACKGROUND: Few studies have examined whether skeletal parameters predict hamstring graft size during anterior cruciate ligament reconstruction (ACLR). PURPOSE/HYPOTHESIS: The purpose of this study was to examine whether preoperative anthropometric and radiographic skeletal parameters could predict hamstring graft size during ACLR. We hypothesized that both anthropometric and skeletal parameters can be used to predict graft size in our double-bundle procedure and that the use of skeletal parameters will improve the accuracy of graft size prediction...
August 2017: Orthopaedic Journal of Sports Medicine
https://www.readbyqxmd.com/read/28807922/using-population-pharmacokinetic-modeling-and-monte-carlo-simulations-to-determine-whether-standard-doses-of-piperacillin-in-piperacillin-tazobactam-regimens-are-adequate-for-the-management-of-febrile-neutropenia
#18
Fekade Bruck Sime, Uwe Hahn, Morgyn S Warner, Ing Soo Tiong, Michael S Roberts, Jeffrey Lipman, Sandra L Peake, Jason A Roberts
Changes in the pharmacokinetics of piperacillin in febrile neutropenic patients have been reported to result in suboptimal exposures. This study aimed to develop a population pharmacokinetic model for piperacillin and perform dosing simulation to describe optimal dosing regimens for hematological malignancy patients with febrile neutropenia. Concentration-time data were obtained from previous prospective observational pharmacokinetic and interventional therapeutic drug monitoring studies. Nonparametric population pharmacokinetic analysis and Monte Carlo dosing simulations were performed with the Pmetrics package for R...
November 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28799895/arginine-and-aerobic-training-prevent-endothelial-and-metabolic-alterations-in-rats-at-high-risk-for-the-development-of-the-metabolic-syndrome
#19
Renata F Medeiros, Thaiane G Gaique, Thais Bento-Bernardes, Raquel Kindlovits, Tamiris M B Gomes, Nadia Alice V Motta, Fernanda Carla Brito, Caroline Fernandes-Santos, Karen J Oliveira, Antonio Claudio L Nóbrega
Endothelial function is a key mechanism in the development of CVD. Arginine and exercise are important non-pharmacological strategies for mitigating the impact of metabolic changes in the metabolic syndrome, but the effect of their combined administration is unknown. Thus, the aim of this study was to investigate the isolated and combined effects of aerobic training and arginine supplementation on metabolic variables and vascular reactivity in rats at high risk for developing the metabolic syndrome. Wistar rats were divided into two groups: control and fructose (F - water with 10 % fructose)...
July 2017: British Journal of Nutrition
https://www.readbyqxmd.com/read/28769140/aortic-aneurysm-in-a-patient-with-syphilis-related-spinal-pain-and-paraplegia
#20
Daniel Brito de Araujo, Danise Senna Oliveira, Rodrigo Kraft Rovere, Umberto Lopes de Oliveira Filho
The tertiary stage of syphilis is nowadays extremely rare, showing predilection for the cardiovascular and nervous systems. A 57-year-old Caucasian man sought medical assistance due to back pain that evolved to paraplegia of the lower limbs. A thoracic CT scan demonstrated an important aneurysmatic lesion of the descending thoracic aorta causing erosion of the vertebral bodies and VDRL and FTA-abs positivity. Although rare, syphilitic aortitis, the hallmark of cardiovascular syphilis, should be considered in the differential diagnosis in patients with thoracic aneurysm when in the absence of classic risk factors for atherosclerosis, especially in cases that progress with erosion of vertebral bodies...
2017: Reumatologia
keyword
keyword
12380
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"