keyword
MENU ▼
Read by QxMD icon Read
search

FTA

keyword
https://www.readbyqxmd.com/read/28343098/developmental-validation-of-qiagen-investigator-%C3%A2-24plex-qs-kit-and-investigator-%C3%A2-24plex-go-kit-two-6-dye-multiplex-assays-for-the-extended-codis-core-loci
#1
Melanie Kraemer, Anke Prochnow, Michael Bussmann, Mario Scherer, Ralf Peist, Carolyn Steffen
The original CODIS database based on 13 core STR loci has been overwhelmingly successful for matching suspects with evidence. In order to increase the power of discrimination, reduce the possibility of adventitious matches, and expand global data sharing, the CODIS Core Loci Working Group determined the expansion of the CODIS core loci to 20 STR plus three additional "highly recommended" loci (SE33, DY391, Amelogenin) Hares, 2015, 2012 [1,2]. The QIAGEN Investigator 24plex QS and Investigator 24plex GO! Kits are 6-dye multiplex assays that contain all markers of the expanded 23 CODIS core loci along with a unique internal performance control that is co-amplified with the STR markers...
March 12, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28324958/ultrafast-oscillatory-flow-pcr-for-amplication-of-urine-short-tandem-repeats
#2
Haoyu Tang, Tao Dong
The short tandem repeats (STRs) in human urine which is the most sensitive body fluid can be serviced as biomarkers for the individual identification. PCR microchips are automated, sensitive and rapid. In this report, COMSOL with Finite Element Analysis (FEA) is employed to study the temperature distribution of the base layer to optimize the structure of the microfluidic chip. Meanwhile, the oscillatory PCR microdevice is preliminary tested by DNA samples extracted from urine with the FTA papers and CSF1PO, TPOX, TH01 Multiplex kits...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28289733/correcting-for-microbial-blooms-in-fecal-samples-during-room-temperature-shipping
#3
Amnon Amir, Daniel McDonald, Jose A Navas-Molina, Justine Debelius, James T Morton, Embriette Hyde, Adam Robbins-Pianka, Rob Knight
The use of sterile swabs is a convenient and common way to collect microbiome samples, and many studies have shown that the effects of room-temperature storage are smaller than physiologically relevant differences between subjects. However, several bacterial taxa, notably members of the class Gammaproteobacteria, grow at room temperature, sometimes confusing microbiome results, particularly when stability is assumed. Although comparative benchmarking has shown that several preservation methods, including the use of 95% ethanol, fecal occult blood test (FOBT) and FTA cards, and Omnigene-GUT kits, reduce changes in taxon abundance during room-temperature storage, these techniques all have drawbacks and cannot be applied retrospectively to samples that have already been collected...
March 2017: MSystems
https://www.readbyqxmd.com/read/28285525/geochemical-and-hydrologic-factors-controlling-subsurface-transport-of-poly-and-perfluoroalkyl-substances-cape-cod-massachusetts
#4
Andrea Kristina Weber, Larry B Barber, Denis R LeBlanc, Elsie M Sunderland, Chad David Vecitis
Growing evidence that certain poly- and perfluoroalkyl substances (PFASs) are associated with negative human health effects prompted the U.S. Environmental Protection Agency to issue lifetime drinking water health advisories for perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) in 2016. Given that groundwater is a major source of drinking water, the main objective of this work was to investigate geochemical and hydrological processes governing the subsurface transport of PFASs at a former fire training area (FTA) on Cape Cod, Massachusetts, where PFAS-containing aqueous film-forming foams were used historically...
March 13, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28277871/meta-analysis-of-pemetrexed-plus-carboplatin-doublet-safety-profile-in-first-line-non-squamous-non-small-cell-lung-cancer-studies
#5
Isamu Okamoto, Wolfgang H W Schuette, Thomas E Stinchcombe, José Rodrigues-Pereira, Belén San Antonio, Jian Chen, Jingyi Liu, William J John, Ralph G Zinner
OBJECTIVES: This meta-analysis compared safety profiles (selected drug-related treatment-emergent adverse events [TEAEs]) of first-line pemetrexed plus carboplatin (PCb) area under the concentration-time curve 5 mg/min•mL (PCb5) or 6 mg/min•mL (PCb6), two widely used regimens in clinical practice for advanced non-squamous non-small cell lung cancer. METHODS: All patients received pemetrexed 500 mg/m(2) every 21 days with either of two carboplatin doses for up to 4-6 cycles...
March 12, 2017: Current Medical Research and Opinion
https://www.readbyqxmd.com/read/28235677/optimizing-direct-amplification-of-forensic-commercial-kits-for-str-determination
#6
M Caputo, M C Bobillo, A Sala, D Corach
Direct DNA amplification in forensic genotyping reduces analytical time when large sample sets are being analyzed. The amplification success depends mainly upon two factors: on one hand, the PCR chemistry and, on the other, the type of solid substrate where the samples are deposited. We developed a workflow strategy aiming to optimize times and cost when starting from blood samples spotted onto diverse absorbent substrates. A set of 770 blood samples spotted onto Blood cards, Whatman(®) 3 MM paper, FTA™ Classic cards, and Whatman(®) Grade 1 was analyzed by a unified working strategy including a low-cost pre-treatment, a PCR amplification volume scale-down, and the use of the 3500 Genetic Analyzer as the analytical platform...
January 25, 2017: Journal of Forensic and Legal Medicine
https://www.readbyqxmd.com/read/28224056/evaluation-and-comparison-of-fta-card-and-ctab-dna-extraction-methods-for-non-agricultural-taxa
#7
Chloe S Siegel, Florence O Stevenson, Elizabeth A Zimmer
PREMISE OF THE STUDY: An efficient, effective DNA extraction method is necessary for comprehensive analysis of plant genomes. This study analyzed the quality of DNA obtained using paper FTA cards prepared directly in the field when compared to the more traditional cetyltrimethylammonium bromide (CTAB)-based extraction methods from silica-dried samples. METHODS: DNA was extracted using FTA cards according to the manufacturer's protocol. In parallel, CTAB-based extractions were done using the automated AutoGen DNA isolation system...
February 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28171796/increased-recovery-of-touch-dna-evidence-using-fta-paper-compared-to-conventional-collection-methods
#8
Irina A Kirgiz, Cassandra Calloway
Tape lifting and FTA paper scraping methods were directly compared to traditional double swabbing for collecting touch DNA from car steering wheels (n = 70 cars). Touch DNA was collected from the left or right side of each steering wheel (randomized) using two sterile cotton swabs, while the other side was sampled using water-soluble tape or FTA paper cards. DNA was extracted and quantified in duplicate using qPCR. Quantifiable amounts of DNA were detected for 100% of the samples (n = 140) collected independent of the method...
January 31, 2017: Journal of Forensic and Legal Medicine
https://www.readbyqxmd.com/read/28171784/developmental-validation-of-the-miseq-fgx-forensic-genomics-system-for-targeted-next-generation-sequencing-in-forensic-dna-casework-and-database-laboratories
#9
Anne C Jäger, Michelle L Alvarez, Carey P Davis, Ernesto Guzmán, Yonmee Han, Lisa Way, Paulina Walichiewicz, David Silva, Nguyen Pham, Glorianna Caves, Jocelyne Bruand, Felix Schlesinger, Stephanie J K Pond, Joe Varlaro, Kathryn M Stephens, Cydne L Holt
Human DNA profiling using PCR at polymorphic short tandem repeat (STR) loci followed by capillary electrophoresis (CE) size separation and length-based allele typing has been the standard in the forensic community for over 20 years. Over the last decade, Next-Generation Sequencing (NGS) matured rapidly, bringing modern advantages to forensic DNA analysis. The MiSeq FGx™ Forensic Genomics System, comprised of the ForenSeq™ DNA Signature Prep Kit, MiSeq FGx™ Reagent Kit, MiSeq FGx™ instrument and ForenSeq™ Universal Analysis Software, uses PCR to simultaneously amplify up to 231 forensic loci in a single multiplex reaction...
January 27, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28143557/factors-affecting-one-leg-standing-time-in-patients-with-end-stage-knee-osteoarthritis-and-the-age-related-recovery-process-following-total-knee-arthroplasty
#10
Kengo Harato, Shu Kobayashi, Iwao Kojima, Aiko Sakurai, Hidenori Tanikawa, Yasuo Niki
BACKGROUND: The aims of the present study were to investigate the factors affecting one-leg standing (OLS) time in patients with end-stage knee osteoarthritis (OA) and to clarify the age-related recovery process following total knee arthroplasty (TKA) in the early postoperative period. METHODS: A total of 80 knees of 40 patients with knee OA were enrolled. They were asked to perform relaxed standing on one leg for as long as possible. First, OLS time was measured...
February 1, 2017: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/28132085/tibial-condylar-valgus-osteotomy-tcvo-for-osteoarthritis-of-the-knee-5-year-clinical-and-radiological-results
#11
Ko Chiba, Akihiko Yonekura, Takashi Miyamoto, Makoto Osaki, Goji Chiba
PURPOSE: Tibial condylar valgus osteotomy (TCVO) is a type of opening-wedge high tibial osteotomy for advanced medial knee osteoarthritis (OA) with subluxated lateral joint. We report the concept, the current surgical technique with a locking plate, and the short-term clinical and radiological results of this procedure. METHODS: 11 knees with medial OA and a widened lateral joint were treated by TCVO (KL stage III: 6, IV: 5). In this procedure, by the L-shaped osteotomy from the medial side of the proximal tibia to the intercondylar eminence and the valgus correction, lateralization of the mechanical axis and reduction of the subluxated lateral joint are obtained with early postoperative weight-bearing...
March 2017: Archives of Orthopaedic and Trauma Surgery
https://www.readbyqxmd.com/read/28127369/molecular-identification-of-hemoprotozoan-parasites-in-camels-camelus-dromedarius-of-iran
#12
Alireza Sazmand, Barbara Eigner, Mohammad Mirzaei, Seyed Hossein Hekmatimoghaddam, Josef Harl, Georg Gerhard Duscher, Hans-Peter Fuehrer, Anja Joachim
BACKGROUND: Although camels represent a valuable source of food, wool and hide in many countries, in-depth information about their vector-borne pathogens is scarce compared to other animals. The aim of the current study was to characterize vector-borne protozoa in the blood of dromedaries from Iran by molecular tools. METHODS: From June to July 2014, 200 peripheral blood samples were collected from asymptomatic one-humped camels in two provinces of Kerman and Sistan- va-Baloochestan in central and southeastern Iran...
October 2016: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28127357/simplified-pan-species-real-time-pcr-based-detection-of-plasmodium-spp-in-blood-smear
#13
Gholamreza Hassanpour, Hossein Mirhendi, Mehdi Mohebali, Ahmad Raeisi, Hojjat Zeraati, Hossein Keshavarz
BACKGROUND: We aimed to quicken and simplify the detection of Plasmodium in blood samples by developing and testing a pan-Plasmodium real-time PCR for accurate screening of individuals suspected of malaria. METHODS: A single primer/probe set for pan-species Plasmodium-specific real time PCR targeting a conserved region of the small subunit 18S ribosomal DNA was designed and evaluated for rapid diagnosis and screening of malaria infections using dried blood smears...
October 2016: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28107347/whole-genome-amplification-and-reduced-representation-genome-sequencing-of-schistosoma-japonicum-miracidia
#14
Jonathan A Shortt, Daren C Card, Drew R Schield, Yang Liu, Bo Zhong, Todd A Castoe, Elizabeth J Carlton, David D Pollock
BACKGROUND: In areas where schistosomiasis control programs have been implemented, morbidity and prevalence have been greatly reduced. However, to sustain these reductions and move towards interruption of transmission, new tools for disease surveillance are needed. Genomic methods have the potential to help trace the sources of new infections, and allow us to monitor drug resistance. Large-scale genotyping efforts for schistosome species have been hindered by cost, limited numbers of established target loci, and the small amount of DNA obtained from miracidia, the life stage most readily acquired from humans...
January 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28073899/paper-based-microrna-expression-profiling-from-plasma-and-circulating-tumor-cells
#15
Sai Mun Leong, Karen Mei-Ling Tan, Hui Wen Chua, Mo-Chao Huang, Wai Chye Cheong, Mo-Huang Li, Steven Tucker, Evelyn Siew-Chuan Koay
BACKGROUND: Molecular characterization of circulating tumor cells (CTCs) holds great promise for monitoring metastatic progression and characterizing metastatic disease. However, leukocyte and red blood cell contamination of routinely isolated CTCs makes CTC-specific molecular characterization extremely challenging. METHODS: Here we report the use of a paper-based medium for efficient extraction of microRNAs (miRNAs) from limited amounts of biological samples such as rare CTCs harvested from cancer patient blood...
January 10, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28048580/we-b-brc-00-concepts-in-risk-based-assessment
#16
Benedick Fraass
Prospective quality management techniques, long used by engineering and industry, have become a growing aspect of efforts to improve quality management and safety in healthcare. These techniques are of particular interest to medical physics as scope and complexity of clinical practice continue to grow, thus making the prescriptive methods we have used harder to apply and potentially less effective for our interconnected and highly complex healthcare enterprise, especially in imaging and radiation oncology. An essential part of most prospective methods is the need to assess the various risks associated with problems, failures, errors, and design flaws in our systems...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28047920/we-b-brc-01-current-methodologies-in-risk-assessment
#17
F Rath
Prospective quality management techniques, long used by engineering and industry, have become a growing aspect of efforts to improve quality management and safety in healthcare. These techniques are of particular interest to medical physics as scope and complexity of clinical practice continue to grow, thus making the prescriptive methods we have used harder to apply and potentially less effective for our interconnected and highly complex healthcare enterprise, especially in imaging and radiation oncology. An essential part of most prospective methods is the need to assess the various risks associated with problems, failures, errors, and design flaws in our systems...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28047253/we-b-brc-02-risk-analysis-and-incident-learning
#18
B Fraass
Prospective quality management techniques, long used by engineering and industry, have become a growing aspect of efforts to improve quality management and safety in healthcare. These techniques are of particular interest to medical physics as scope and complexity of clinical practice continue to grow, thus making the prescriptive methods we have used harder to apply and potentially less effective for our interconnected and highly complex healthcare enterprise, especially in imaging and radiation oncology. An essential part of most prospective methods is the need to assess the various risks associated with problems, failures, errors, and design flaws in our systems...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28046258/we-b-brc-03-risk-in-the-context-of-medical-imaging
#19
E Samei
Prospective quality management techniques, long used by engineering and industry, have become a growing aspect of efforts to improve quality management and safety in healthcare. These techniques are of particular interest to medical physics as scope and complexity of clinical practice continue to grow, thus making the prescriptive methods we have used harder to apply and potentially less effective for our interconnected and highly complex healthcare enterprise, especially in imaging and radiation oncology. An essential part of most prospective methods is the need to assess the various risks associated with problems, failures, errors, and design flaws in our systems...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28017635/use-of-fta-%C3%A2-card-methodology-for-sampling-and-molecular-characterization-of-echinococcus-granulosus-sensu-lato-in-africa
#20
Franck Boué, Ikhlass El Berbri, Vanessa Hormaz, Jean-Marc Boucher, Ahmed Bezeid El Mamy, Abdallah Traore, Ouafaa Fassi Fihri, Anne-Françoise Petavy, Allal Dakkak, Gérald Umhang
Cystic Echinococcosis is a parasitic disease caused by the cestode Echinococcus granulosus widely distributed in Africa. Monitoring of this parasite requires access to cyst samples on intermediate hosts observed at the slaughterhouse. In order to facilitate sampling in the field and analysis, the French National Reference Laboratory for Echinococcus spp. has developed a tissue derived from DNA sampling with FTA(®) card technology. The DNA samples were taken by applying the FTA(®) paper on the germinal layer after opening the cysts...
February 2017: Experimental Parasitology
keyword
keyword
12380
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"