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https://www.readbyqxmd.com/read/28107347/whole-genome-amplification-and-reduced-representation-genome-sequencing-of-schistosoma-japonicum-miracidia
#1
Jonathan A Shortt, Daren C Card, Drew R Schield, Yang Liu, Bo Zhong, Todd A Castoe, Elizabeth J Carlton, David D Pollock
BACKGROUND: In areas where schistosomiasis control programs have been implemented, morbidity and prevalence have been greatly reduced. However, to sustain these reductions and move towards interruption of transmission, new tools for disease surveillance are needed. Genomic methods have the potential to help trace the sources of new infections, and allow us to monitor drug resistance. Large-scale genotyping efforts for schistosome species have been hindered by cost, limited numbers of established target loci, and the small amount of DNA obtained from miracidia, the life stage most readily acquired from humans...
January 20, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28073899/paper-based-microrna-expression-profiling-from-plasma-and-circulating-tumor-cells
#2
Sai Mun Leong, Karen Mei-Ling Tan, Hui Wen Chua, Mo-Chao Huang, Wai Chye Cheong, Mo-Huang Li, Steven Tucker, Evelyn Siew-Chuan Koay
BACKGROUND: Molecular characterization of circulating tumor cells (CTCs) holds great promise for monitoring metastatic progression and characterizing metastatic disease. However, leukocyte and red blood cell contamination of routinely isolated CTCs makes CTC-specific molecular characterization extremely challenging. METHODS: Here we report the use of a paper-based medium for efficient extraction of microRNAs (miRNAs) from limited amounts of biological samples such as rare CTCs harvested from cancer patient blood...
January 10, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28048580/we-b-brc-00-concepts-in-risk-based-assessment
#3
Benedick Fraass
: Prospective quality management techniques, long used by engineering and industry, have become a growing aspect of efforts to improve quality management and safety in healthcare. These techniques are of particular interest to medical physics as scope and complexity of clinical practice continue to grow, thus making the prescriptive methods we have used harder to apply and potentially less effective for our interconnected and highly complex healthcare enterprise, especially in imaging and radiation oncology...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28047920/we-b-brc-01-current-methodologies-in-risk-assessment
#4
F Rath
: Prospective quality management techniques, long used by engineering and industry, have become a growing aspect of efforts to improve quality management and safety in healthcare. These techniques are of particular interest to medical physics as scope and complexity of clinical practice continue to grow, thus making the prescriptive methods we have used harder to apply and potentially less effective for our interconnected and highly complex healthcare enterprise, especially in imaging and radiation oncology...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28047253/we-b-brc-02-risk-analysis-and-incident-learning
#5
B Fraass
: Prospective quality management techniques, long used by engineering and industry, have become a growing aspect of efforts to improve quality management and safety in healthcare. These techniques are of particular interest to medical physics as scope and complexity of clinical practice continue to grow, thus making the prescriptive methods we have used harder to apply and potentially less effective for our interconnected and highly complex healthcare enterprise, especially in imaging and radiation oncology...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28046258/we-b-brc-03-risk-in-the-context-of-medical-imaging
#6
E Samei
: Prospective quality management techniques, long used by engineering and industry, have become a growing aspect of efforts to improve quality management and safety in healthcare. These techniques are of particular interest to medical physics as scope and complexity of clinical practice continue to grow, thus making the prescriptive methods we have used harder to apply and potentially less effective for our interconnected and highly complex healthcare enterprise, especially in imaging and radiation oncology...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28017635/use-of-fta-%C3%A2-card-methodology-for-sampling-and-molecular-characterization-of-echinococcus-granulosus-sensu-lato-in-africa
#7
Franck Boué, Ikhlass El Berbri, Vanessa Hormaz, Jean-Marc Boucher, Ahmed Bezeid El Mamy, Abdallah Traore, Ouafaa Fassi Fihri, Anne-Françoise Petavy, Allal Dakkak, Gérald Umhang
Cystic Echinococcosis is a parasitic disease caused by the cestode Echinococcus granulosus widely distributed in Africa. Monitoring of this parasite requires access to cyst samples on intermediate hosts observed at the slaughterhouse. In order to facilitate sampling in the field and analysis, the French National Reference Laboratory for Echinococcus spp. has developed a tissue derived from DNA sampling with FTA(®) card technology. The DNA samples were taken by applying the FTA(®) paper on the germinal layer after opening the cysts...
December 23, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/28008668/immunoglobulin-heavy-and-light-chains-and-t-cell-receptor-beta-and-gamma-chains-pcr-assessment-on-cytological-samples-a-study-comparing-fta-cards-and-cryopreserved-lymph-node-fine-needle-cytology
#8
A L Peluso, I Cozzolino, A Bottiglieri, L Lucchese, R M Di Crescenzo, M Langella, C Selleri, P Zeppa
OBJECTIVES: To evaluate and compare the DNA yield and quality extracted from lymph node fine needle cytology (FNC) samples stored on FTA cards to those cryopreserved, and to assess the immunoglobulin heavy and light chains (IGHK) and T-Cell receptor beta and gamma chains (TCRBG) PCR tests. METHODS: DNA extractions were performed on FNC of 80 non-Hodgkin lymphomas (NHL), four myelomas and 56 benign reactive hyperplasias (BRH) cryopreserved and stored on FTA cards...
December 22, 2016: Cytopathology: Official Journal of the British Society for Clinical Cytology
https://www.readbyqxmd.com/read/27927929/high-throughput-next-generation-sequencing-of-polioviruses
#9
Anna M Montmayeur, Terry Fei Fan Ng, Alexander Schmidt, Kun Zhao, Laura Magaña, Jane Iber, Christina J Castro, Qi Chen, Elizabeth Henderson, Edward Ramos, Jing Shaw, Roman L Tatusov, Naomi Dybdahl-Sissoko, Marie Claire Endegue-Zanga, Johnson A Adeniji, M Steven Oberste, Cara C Burns
Poliovirus (PV) is currently targeted for worldwide eradication and containment. Sanger-based sequencing of the VP1 capsid region is the current standard method for PV surveillance; however, the whole genome sequence is sometimes needed for higher resolution global surveillance. In this study, we optimized whole genome sequencing protocols for poliovirus isolates and FTA cards using NGS, aiming for high sequence coverage, efficiency, and throughput. We found that DNase treatment of poliovirus RNA followed by random RT, amplification, and the Nextera XT DNA Library Preparation Kit produced significantly better results than other preparations...
December 7, 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27919688/leishmaniases-in-ecuador-comprehensive-review-and-current-status
#10
REVIEW
Yoshihisa Hashiguchi, Lenin N Velez, Nancy V Villegas, Tatsuyuki Mimori, Eduardo A L Gomez, Hirotomo Kato
This article reviews current knowledge about leishmaniases in Ecuador, proceeding from 1920, when the first human case was described, to the present, mainly focusing on the recent research events published. Regarding basic situations, it appears that 23 of Ecuador's 24 provinces have leishmaniasis-case reports. The disease is one of the mandatory notification infectious diseases in the country since 2005. All the 21,305 cases notified to the Ministry of Public Health, during the period from 2001 through 2014, were said to involve different clinical features of cutaneous leishmaniasis (CL) but not visceral (VL)...
February 2017: Acta Tropica
https://www.readbyqxmd.com/read/27889569/an-evaluation-of-transit-procurement-training
#11
Robert B Noland, Marc D Weiner, Nicholas J Klein, Orin D Puniello
We evaluated a training course called "Orientation to Transit Procurement", designed and conducted by the National Transit Institute. This course is designed to provide Federal Transit Administration (FTA) grantees an overview of regulations and best practices related to the procurement process. Our objective in conducting the evaluation was to understand how transit agency staff made changes in procurement practices in response to the course training. The evaluation was mixed mode: an Internet survey followed by in-depth interviews with a small group of respondents...
November 3, 2016: Evaluation and Program Planning
https://www.readbyqxmd.com/read/27846440/evaluation-of-human-papillomavirus-detection-by-abbott-m2000-system-on-samples-collected-by-fta-elute%C3%A2-card-in-a-chinese-hiv-1-positive-population
#12
Yu Qin, Hongyun Zhang, Natalia Marlowe, Mandong Fei, Judy Yu, Xiaoqin Lei, Lulu Yu, Jia Zhang, Di Cao, Li Ma, Wen Chen
BACKGROUND: HIV+/AIDS women have an increased risk of developing into CIN and cervical cancer compared to the general population. Limited medical resource and the lack of AIDS relevant knowledge impair the coverage and efficiency of cervical cancer screening. OBJECTIVES: To compare the clinical performance of self-collected dry storage medium (FTA Elute card) and physician-collected PreservCyt medium in detection of high risk human papillomavirus (HR HPV) among HIV-1 positive population...
November 9, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27825733/a-comparison-of-dna-extraction-protocols-from-blood-spotted-on-fta-cards-for-the-detection-of-tick-borne-pathogens-by-reverse-line-blot-hybridization
#13
Zerihun Hailemariam, Jabbar Sabir Ahmed, Peter-Henning Clausen, Ard Menzo Nijhof
An essential step in the molecular detection of tick-borne pathogens (TBPs) in blood is the extraction of DNA. When cooled storage of blood under field conditions prior to DNA extraction in a dedicated laboratory is not possible, the storage of blood on filter paper forms a promising alternative. We evaluated six DNA extraction methods from blood spotted on FTA Classic(®) cards (FTA cards), to determine the optimal protocol for the subsequent molecular detection of TBPs by PCR and the Reverse Line Blot hybridization assay (RLB)...
January 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27822526/preservation-methods-differ-in-fecal-microbiome-stability-affecting-suitability-for-field-studies
#14
Se Jin Song, Amnon Amir, Jessica L Metcalf, Katherine R Amato, Zhenjiang Zech Xu, Greg Humphrey, Rob Knight
Immediate freezing at -20°C or below has been considered the gold standard for microbiome preservation, yet this approach is not feasible for many field studies, ranging from anthropology to wildlife conservation. Here we tested five methods for preserving human and dog fecal specimens for periods of up to 8 weeks, including such types of variation as freeze-thaw cycles and the high temperature fluctuations often encountered under field conditions. We found that three of the methods-95% ethanol, FTA cards, and the OMNIgene Gut kit-can preserve samples sufficiently well at ambient temperatures such that differences at 8 weeks are comparable to differences among technical replicates...
May 2016: MSystems
https://www.readbyqxmd.com/read/27818966/sexually-transmitted-infections-among-hospitalized-patients-with-human-immunodeficiency-virus-infection-and-acquired-immune-deficiency-syndrome-hiv-aids-in-zahedan-southeastern-iran
#15
Seyed Mohammad Hashemi-Shahri, Batool Sharifi-Mood, Hamid-Reza Kouhpayeh, Javad Moazen, Mohsen Farrokhian, Masoud Salehi
BACKGROUND: Studies show that nearly 40 million people are living with human immunodeficiency virus infection and acquired immune deficiency syndrome (HIV/AIDS) around the world and since the beginning of the epidemic, about 35 million have died from AIDS. Heterosexual intercourse is the most common route for transmission of HIV infection (85%). People with a sexually transmitted infection (STI), such as syphilis, genital herpes, chancroid, or bacterial vaginosis, are more likely to obtain HIV infection during sex...
September 2016: International Journal of High Risk Behaviors & Addiction
https://www.readbyqxmd.com/read/27806753/fault-tree-analysis-investigation-of-epidemic-hemorrhagic-fever-infection-acquired-in-animal-laboratories-in-china
#16
Xiao Yu Liu, Kang Ning Xue, Rong Rong, Chi Hong Zhao
Epidemic hemorrhagic fever has been an ongoing threat to laboratory personnel involved in animal care and use. Laboratory transmissions and severe infections occurred over the past twenty years, even though the standards and regulations for laboratory biosafety have been issued, upgraded, and implemented in China. Therefore, there is an urgent need to identify risk factors and to seek effective preventive measures that can curb the incidences of epidemic hemorrhagic fever among laboratory personnel. In the present study, we reviewed literature that relevant to animals laboratory-acquired hemorrhagic fever infections reported from 1995 to 2015, and analyzed these incidences using fault tree analysis (FTA)...
2016: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/27795491/genetic-profiling-of-two-phenotypically-distinct-outbred-rats-derived-from-a-colony-of-the-zucker-fatty-rats-maintained-at-the-tokyo-medical-university
#17
Satoshi Nakanishi, Takashi Kuramoto, Naomi Kashiwazaki, Norihide Yokoi
The Zucker fatty (ZF) rat is an outbred rat and a well-known model of obesity without diabetes, harboring a missense mutation (fatty, abbreviated as fa) in the leptin receptor gene (Lepr). Slc:Zucker (Slc:ZF) outbred rats exhibit obesity while Hos:ZFDM-Lepr(fa) (Hos:ZFDM) outbred rats exhibit obesity and type 2 diabetes. Both outbred rats have been derived from an outbred ZF rat colony maintained at the Tokyo Medical University. So far, genetic profiles of these outbred rats remain unknown. Here, we applied a simple genotyping method using Ampdirect reagents and FTA cards (Amp-FTA) in combination with simple sequence length polymorphisms (SSLP) markers to determine genetic profiles of Slc:ZF and Hos:ZFDM rats...
October 31, 2016: Experimental Animals
https://www.readbyqxmd.com/read/27787614/molecular-diagnosis-of-the-tick-borne-pathogen-anaplasma-marginale-in-cattle-blood-samples-from-nigeria-using-qpcr
#18
Nusirat Elelu, Joana Ferrolho, Joana Couto, Ana Domingos, Mark C Eisler
Tick-borne diseases (TBDs) are some of the most important animal health and management problems in Africa, including Nigeria. This study aims to determine the prevalence of an important TBD, anaplasmosis, in a North-central region of Nigeria. Blood samples were collected from cattle and stored on Whatman FTA(®) cards. Information on village, age and sex associated with each cattle was also recorded. The packed red blood cell volume (PCV) for each blood sample was determined. After DNA extraction, pathogen presence was evaluated by TaqMan(®) based qPCR of which 75...
December 2016: Experimental & Applied Acarology
https://www.readbyqxmd.com/read/27769166/expansion-of-the-phosphatidylethanolamine-binding-protein-family-in-legumes-a-case-study-of-lupinus-angustifolius-l-flowering-locus-t-homologs-lanftc1-and-lanftc2
#19
Michał Książkiewicz, Sandra Rychel, Matthew N Nelson, Katarzyna Wyrwa, Barbara Naganowska, Bogdan Wolko
BACKGROUND: The Arabidopsis FLOWERING LOCUS T (FT) gene, a member of the phosphatidylethanolamine binding protein (PEBP) family, is a major controller of flowering in response to photoperiod, vernalization and light quality. In legumes, FT evolved into three, functionally diversified clades, FTa, FTb and FTc. A milestone achievement in narrow-leafed lupin (Lupinus angustifolius L.) domestication was the loss of vernalization responsiveness at the Ku locus. Recently, one of two existing L...
October 21, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27716955/computation-of-femoral-canine-morphometric-parameters-in-three-dimensional-geometrical-models
#20
Gianpaolo Savio, Teresa Baroni, Gianmaria Concheri, Ermenegildo Baroni, Roberto Meneghello, Federico Longo, Maurizio Isola
OBJECTIVE: To define and validate a method for the measurement of 3-dimensional (3D) morphometric parameters in polygonal mesh models of canine femora. STUDY DESIGN: Ex vivo/computerized model. SAMPLE POPULATION: Sixteen femora from 8 medium to large-breed canine cadavers (mean body weight 28.3 kg, mean age 5.3 years). METHODS: Femora were measured with a 3D scanner, obtaining 3D meshes. A computer-aided design-based (CAD) software tool was purposely developed, which allowed automatic calculation of morphometric parameters on a mesh model...
September 26, 2016: Veterinary Surgery: VS
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