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https://www.readbyqxmd.com/read/28171796/increased-recovery-of-touch-dna-evidence-using-fta-paper-compared-to-conventional-collection-methods
#1
Irina A Kirgiz, Cassandra Calloway
Tape lifting and FTA paper scraping methods were directly compared to traditional double swabbing for collecting touch DNA from car steering wheels (n = 70 cars). Touch DNA was collected from the left or right side of each steering wheel (randomized) using two sterile cotton swabs, while the other side was sampled using water-soluble tape or FTA paper cards. DNA was extracted and quantified in duplicate using qPCR. Quantifiable amounts of DNA were detected for 100% of the samples (n = 140) collected independent of the method...
January 31, 2017: Journal of Forensic and Legal Medicine
https://www.readbyqxmd.com/read/28171784/developmental-validation-of-the-miseq-fgx-forensic-genomics-system-for-targeted-next-generation-sequencing-in-forensic-dna-casework-and-database-laboratories
#2
Anne C Jäger, Michelle L Alvarez, Carey P Davis, Ernesto Guzmán, Yonmee Han, Lisa Way, Paulina Walichiewicz, David Silva, Nguyen Pham, Glorianna Caves, Jocelyne Bruand, Felix Schlesinger, Stephanie J K Pond, Joe Varlaro, Kathryn M Stephens, Cydne L Holt
Human DNA profiling using PCR at polymorphic short tandem repeat (STR) loci followed by capillary electrophoresis (CE) size separation and length-based allele typing has been the standard in the forensic community for over 20 years. Over the last decade, Next-Generation Sequencing (NGS) matured rapidly, bringing modern advantages to forensic DNA analysis. The MiSeq FGx™ Forensic Genomics System, comprised of the ForenSeq™ DNA Signature Prep Kit, MiSeq FGx™ Reagent Kit, MiSeq FGx™ instrument and ForenSeq™ Universal Analysis Software, uses PCR to simultaneously amplify up to 231 forensic loci in a single multiplex reaction...
January 27, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28143557/factors-affecting-one-leg-standing-time-in-patients-with-end-stage-knee-osteoarthritis-and-the-age-related-recovery-process-following-total-knee-arthroplasty
#3
Kengo Harato, Shu Kobayashi, Iwao Kojima, Aiko Sakurai, Hidenori Tanikawa, Yasuo Niki
BACKGROUND: The aims of the present study were to investigate the factors affecting one-leg standing (OLS) time in patients with end-stage knee osteoarthritis (OA) and to clarify the age-related recovery process following total knee arthroplasty (TKA) in the early postoperative period. METHODS: A total of 80 knees of 40 patients with knee OA were enrolled. They were asked to perform relaxed standing on one leg for as long as possible. First, OLS time was measured...
February 1, 2017: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/28132085/tibial-condylar-valgus-osteotomy-tcvo-for-osteoarthritis-of-the-knee-5-year-clinical-and-radiological-results
#4
Ko Chiba, Akihiko Yonekura, Takashi Miyamoto, Makoto Osaki, Goji Chiba
PURPOSE: Tibial condylar valgus osteotomy (TCVO) is a type of opening-wedge high tibial osteotomy for advanced medial knee osteoarthritis (OA) with subluxated lateral joint. We report the concept, the current surgical technique with a locking plate, and the short-term clinical and radiological results of this procedure. METHODS: 11 knees with medial OA and a widened lateral joint were treated by TCVO (KL stage III: 6, IV: 5). In this procedure, by the L-shaped osteotomy from the medial side of the proximal tibia to the intercondylar eminence and the valgus correction, lateralization of the mechanical axis and reduction of the subluxated lateral joint are obtained with early postoperative weight-bearing...
January 28, 2017: Archives of Orthopaedic and Trauma Surgery
https://www.readbyqxmd.com/read/28127369/molecular-identification-of-hemoprotozoan-parasites-in-camels-camelus-dromedarius-of-iran
#5
Alireza Sazmand, Barbara Eigner, Mohammad Mirzaei, Seyed Hossein Hekmatimoghaddam, Josef Harl, Georg Gerhard Duscher, Hans-Peter Fuehrer, Anja Joachim
BACKGROUND: Although camels represent a valuable source of food, wool and hide in many countries, in-depth information about their vector-borne pathogens is scarce compared to other animals. The aim of the current study was to characterize vector-borne protozoa in the blood of dromedaries from Iran by molecular tools. METHODS: From June to July 2014, 200 peripheral blood samples were collected from asymptomatic one-humped camels in two provinces of Kerman and Sistan- va-Baloochestan in central and southeastern Iran...
October 2016: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28127357/simplified-pan-species-real-time-pcr-based-detection-of-plasmodium-spp-in-blood-smear
#6
Gholamreza Hassanpour, Hossein Mirhendi, Mehdi Mohebali, Ahmad Raeisi, Hojjat Zeraati, Hossein Keshavarz
BACKGROUND: We aimed to quicken and simplify the detection of Plasmodium in blood samples by developing and testing a pan-Plasmodium real-time PCR for accurate screening of individuals suspected of malaria. METHODS: A single primer/probe set for pan-species Plasmodium-specific real time PCR targeting a conserved region of the small subunit 18S ribosomal DNA was designed and evaluated for rapid diagnosis and screening of malaria infections using dried blood smears...
October 2016: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28107347/whole-genome-amplification-and-reduced-representation-genome-sequencing-of-schistosoma-japonicum-miracidia
#7
Jonathan A Shortt, Daren C Card, Drew R Schield, Yang Liu, Bo Zhong, Todd A Castoe, Elizabeth J Carlton, David D Pollock
BACKGROUND: In areas where schistosomiasis control programs have been implemented, morbidity and prevalence have been greatly reduced. However, to sustain these reductions and move towards interruption of transmission, new tools for disease surveillance are needed. Genomic methods have the potential to help trace the sources of new infections, and allow us to monitor drug resistance. Large-scale genotyping efforts for schistosome species have been hindered by cost, limited numbers of established target loci, and the small amount of DNA obtained from miracidia, the life stage most readily acquired from humans...
January 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28073899/paper-based-microrna-expression-profiling-from-plasma-and-circulating-tumor-cells
#8
Sai Mun Leong, Karen Mei-Ling Tan, Hui Wen Chua, Mo-Chao Huang, Wai Chye Cheong, Mo-Huang Li, Steven Tucker, Evelyn Siew-Chuan Koay
BACKGROUND: Molecular characterization of circulating tumor cells (CTCs) holds great promise for monitoring metastatic progression and characterizing metastatic disease. However, leukocyte and red blood cell contamination of routinely isolated CTCs makes CTC-specific molecular characterization extremely challenging. METHODS: Here we report the use of a paper-based medium for efficient extraction of microRNAs (miRNAs) from limited amounts of biological samples such as rare CTCs harvested from cancer patient blood...
January 10, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28048580/we-b-brc-00-concepts-in-risk-based-assessment
#9
Benedick Fraass
: Prospective quality management techniques, long used by engineering and industry, have become a growing aspect of efforts to improve quality management and safety in healthcare. These techniques are of particular interest to medical physics as scope and complexity of clinical practice continue to grow, thus making the prescriptive methods we have used harder to apply and potentially less effective for our interconnected and highly complex healthcare enterprise, especially in imaging and radiation oncology...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28047920/we-b-brc-01-current-methodologies-in-risk-assessment
#10
F Rath
: Prospective quality management techniques, long used by engineering and industry, have become a growing aspect of efforts to improve quality management and safety in healthcare. These techniques are of particular interest to medical physics as scope and complexity of clinical practice continue to grow, thus making the prescriptive methods we have used harder to apply and potentially less effective for our interconnected and highly complex healthcare enterprise, especially in imaging and radiation oncology...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28047253/we-b-brc-02-risk-analysis-and-incident-learning
#11
B Fraass
: Prospective quality management techniques, long used by engineering and industry, have become a growing aspect of efforts to improve quality management and safety in healthcare. These techniques are of particular interest to medical physics as scope and complexity of clinical practice continue to grow, thus making the prescriptive methods we have used harder to apply and potentially less effective for our interconnected and highly complex healthcare enterprise, especially in imaging and radiation oncology...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28046258/we-b-brc-03-risk-in-the-context-of-medical-imaging
#12
E Samei
: Prospective quality management techniques, long used by engineering and industry, have become a growing aspect of efforts to improve quality management and safety in healthcare. These techniques are of particular interest to medical physics as scope and complexity of clinical practice continue to grow, thus making the prescriptive methods we have used harder to apply and potentially less effective for our interconnected and highly complex healthcare enterprise, especially in imaging and radiation oncology...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28017635/use-of-fta-%C3%A2-card-methodology-for-sampling-and-molecular-characterization-of-echinococcus-granulosus-sensu-lato-in-africa
#13
Franck Boué, Ikhlass El Berbri, Vanessa Hormaz, Jean-Marc Boucher, Ahmed Bezeid El Mamy, Abdallah Traore, Ouafaa Fassi Fihri, Anne-Françoise Petavy, Allal Dakkak, Gérald Umhang
Cystic Echinococcosis is a parasitic disease caused by the cestode Echinococcus granulosus widely distributed in Africa. Monitoring of this parasite requires access to cyst samples on intermediate hosts observed at the slaughterhouse. In order to facilitate sampling in the field and analysis, the French National Reference Laboratory for Echinococcus spp. has developed a tissue derived from DNA sampling with FTA(®) card technology. The DNA samples were taken by applying the FTA(®) paper on the germinal layer after opening the cysts...
December 23, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/28008668/immunoglobulin-heavy-and-light-chains-and-t-cell-receptor-beta-and-gamma-chains-pcr-assessment-on-cytological-samples-a-study-comparing-fta-cards-and-cryopreserved-lymph-node-fine-needle-cytology
#14
A L Peluso, I Cozzolino, A Bottiglieri, L Lucchese, R M Di Crescenzo, M Langella, C Selleri, P Zeppa
OBJECTIVES: To evaluate and compare the DNA yield and quality extracted from lymph node fine needle cytology (FNC) samples stored on FTA cards to those cryopreserved, and to assess the immunoglobulin heavy and light chains (IGHK) and T-Cell receptor beta and gamma chains (TCRBG) PCR tests. METHODS: DNA extractions were performed on FNC of 80 non-Hodgkin lymphomas (NHL), four myelomas and 56 benign reactive hyperplasias (BRH) cryopreserved and stored on FTA cards...
December 22, 2016: Cytopathology: Official Journal of the British Society for Clinical Cytology
https://www.readbyqxmd.com/read/27927929/high-throughput-next-generation-sequencing-of-polioviruses
#15
Anna M Montmayeur, Terry Fei Fan Ng, Alexander Schmidt, Kun Zhao, Laura Magaña, Jane Iber, Christina J Castro, Qi Chen, Elizabeth Henderson, Edward Ramos, Jing Shaw, Roman L Tatusov, Naomi Dybdahl-Sissoko, Marie Claire Endegue-Zanga, Johnson A Adeniji, M Steven Oberste, Cara C Burns
The poliovirus (PV) is currently targeted for worldwide eradication and containment. Sanger-based sequencing of the viral protein 1 (VP1) capsid region is currently the standard method for PV surveillance. However, the whole-genome sequence is sometimes needed for higher resolution global surveillance. In this study, we optimized whole-genome sequencing protocols for poliovirus isolates and FTA cards using next-generation sequencing (NGS), aiming for high sequence coverage, efficiency, and throughput. We found that DNase treatment of poliovirus RNA followed by random reverse transcription (RT), amplification, and the use of the Nextera XT DNA library preparation kit produced significantly better results than other preparations...
February 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27919688/leishmaniases-in-ecuador-comprehensive-review-and-current-status
#16
REVIEW
Yoshihisa Hashiguchi, Lenin N Velez, Nancy V Villegas, Tatsuyuki Mimori, Eduardo A L Gomez, Hirotomo Kato
This article reviews current knowledge about leishmaniases in Ecuador, proceeding from 1920, when the first human case was described, to the present, mainly focusing on the recent research events published. Regarding basic situations, it appears that 23 of Ecuador's 24 provinces have leishmaniasis-case reports. The disease is one of the mandatory notification infectious diseases in the country since 2005. All the 21,305 cases notified to the Ministry of Public Health, during the period from 2001 through 2014, were said to involve different clinical features of cutaneous leishmaniasis (CL) but not visceral (VL)...
February 2017: Acta Tropica
https://www.readbyqxmd.com/read/27889569/an-evaluation-of-transit-procurement-training
#17
Robert B Noland, Marc D Weiner, Nicholas J Klein, Orin D Puniello
We evaluated a training course called "Orientation to Transit Procurement", designed and conducted by the National Transit Institute. This course is designed to provide Federal Transit Administration (FTA) grantees an overview of regulations and best practices related to the procurement process. Our objective in conducting the evaluation was to understand how transit agency staff made changes in procurement practices in response to the course training. The evaluation was mixed mode: an Internet survey followed by in-depth interviews with a small group of respondents...
November 3, 2016: Evaluation and Program Planning
https://www.readbyqxmd.com/read/27846440/evaluation-of-human-papillomavirus-detection-by-abbott-m2000-system-on-samples-collected-by-fta-elute%C3%A2-card-in-a-chinese-hiv-1-positive-population
#18
Yu Qin, Hongyun Zhang, Natalia Marlowe, Mandong Fei, Judy Yu, Xiaoqin Lei, Lulu Yu, Jia Zhang, Di Cao, Li Ma, Wen Chen
BACKGROUND: HIV+/AIDS women have an increased risk of developing into CIN and cervical cancer compared to the general population. Limited medical resource and the lack of AIDS relevant knowledge impair the coverage and efficiency of cervical cancer screening. OBJECTIVES: To compare the clinical performance of self-collected dry storage medium (FTA Elute card) and physician-collected PreservCyt medium in detection of high risk human papillomavirus (HR HPV) among HIV-1 positive population...
November 9, 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27825733/a-comparison-of-dna-extraction-protocols-from-blood-spotted-on-fta-cards-for-the-detection-of-tick-borne-pathogens-by-reverse-line-blot-hybridization
#19
Zerihun Hailemariam, Jabbar Sabir Ahmed, Peter-Henning Clausen, Ard Menzo Nijhof
An essential step in the molecular detection of tick-borne pathogens (TBPs) in blood is the extraction of DNA. When cooled storage of blood under field conditions prior to DNA extraction in a dedicated laboratory is not possible, the storage of blood on filter paper forms a promising alternative. We evaluated six DNA extraction methods from blood spotted on FTA Classic(®) cards (FTA cards), to determine the optimal protocol for the subsequent molecular detection of TBPs by PCR and the Reverse Line Blot hybridization assay (RLB)...
January 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27822526/preservation-methods-differ-in-fecal-microbiome-stability-affecting-suitability-for-field-studies
#20
Se Jin Song, Amnon Amir, Jessica L Metcalf, Katherine R Amato, Zhenjiang Zech Xu, Greg Humphrey, Rob Knight
Immediate freezing at -20°C or below has been considered the gold standard for microbiome preservation, yet this approach is not feasible for many field studies, ranging from anthropology to wildlife conservation. Here we tested five methods for preserving human and dog fecal specimens for periods of up to 8 weeks, including such types of variation as freeze-thaw cycles and the high temperature fluctuations often encountered under field conditions. We found that three of the methods-95% ethanol, FTA cards, and the OMNIgene Gut kit-can preserve samples sufficiently well at ambient temperatures such that differences at 8 weeks are comparable to differences among technical replicates...
May 2016: MSystems
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