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Michał Książkiewicz, Sandra Rychel, Matthew N Nelson, Katarzyna Wyrwa, Barbara Naganowska, Bogdan Wolko
BACKGROUND: The Arabidopsis FLOWERING LOCUS T (FT) gene, a member of the phosphatidylethanolamine binding protein (PEBP) family, is a major controller of flowering in response to photoperiod, vernalization and light quality. In legumes, FT evolved into three, functionally diversified clades, FTa, FTb and FTc. A milestone achievement in narrow-leafed lupin (Lupinus angustifolius L.) domestication was the loss of vernalization responsiveness at the Ku locus. Recently, one of two existing L...
October 21, 2016: BMC Genomics
Gianpaolo Savio, Teresa Baroni, Gianmaria Concheri, Ermenegildo Baroni, Roberto Meneghello, Federico Longo, Maurizio Isola
OBJECTIVE: To define and validate a method for the measurement of 3-dimensional (3D) morphometric parameters in polygonal mesh models of canine femora. STUDY DESIGN: Ex vivo/computerized model. SAMPLE POPULATION: Sixteen femora from 8 medium to large-breed canine cadavers (mean body weight 28.3 kg, mean age 5.3 years). METHODS: Femora were measured with a 3D scanner, obtaining 3D meshes. A computer-aided design-based (CAD) software tool was purposely developed, which allowed automatic calculation of morphometric parameters on a mesh model...
September 26, 2016: Veterinary Surgery: VS
C E Scantlebury, G L Pinchbeck, P Loughnane, N Aklilu, T Ashine, A P Stringer, L Gordon, M Marshall, R M Christley, A J McCarthy
Histoplasma capsulatum var. farciminosum (HCF), the causative agent of epizootic lymphangitis (EZL), is endemic in parts of Africa. Diagnosis based on clinical signs and microscopy lacks specificity, and is a barrier to further understanding this neglected disease. Here, a nested PCR method targeting the ITS region of the rRNA operon was validated for application to equine clinical samples. Twenty-nine horses with signs of EZL, from different climatic regions of Ethiopia, were clinically examined. Blood samples and aspirates of pus from cutaneous nodules were taken, along with blood from a further 20 horses with no cutaneous EZL lesions...
October 5, 2016: Journal of Clinical Microbiology
Chao Zou, Yimin Wang, James A Moore, Masoud Sanayei
Transit-oriented development, such as metro depot and over-track building complexes, has expanded rapidly over the last 5years in China. Over-track building construction has the advantage of comprehensive utilization of land resources, ease of commuting to work, and provide funds for subway construction. But the high frequency of subway operations into and out of the depots can generate excessive vibrations that transmit into the over track buildings, radiate noise within the buildings, hamper the operation of vibration sensitive equipment, and adversely affect the living quality of the building occupants...
October 1, 2016: Science of the Total Environment
Rebecca Moyer, Wolfgang Wirth, Felix Eckstein
OBJECTIVE: To investigate the interaction between malalignment and body mass index (BMI) on cartilage thickness change in patients with knee osteoarthritis (OA). METHODS: Femorotibial cartilage thickness was measured from baseline to 2-years in 558 knees with radiographic OA. Cartilage thickness was determined in the central weight-bearing medial (cMFTC) and lateral (cLFTC) compartments. Femorotibial angle (FTA) was stratified into neutral, minor and definite malalignment...
October 1, 2016: Arthritis Care & Research
Vanessa L Hale, Chia L Tan, Kefeng Niu, Yeqin Yang, Duoying Cui, Hongxia Zhao, Rob Knight, Katherine R Amato
Gut microbiota can provide great insight into host health, and studies of the gut microbiota in wildlife are becoming more common. However, the effects of field conditions on gut microbial samples are unknown. This study addresses the following questions: 1) How do environmental factors such as sunlight and insect infestations affect fecal microbial DNA? 2) How does fecal microbial DNA change over time after defecation? 3) How does storage method affect microbial DNA? Fresh fecal samples were collected, pooled, and homogenized from a family group of 6 spider monkeys, Ateles geoffroyi...
November 2016: Journal of Microbiological Methods
Peng Chen, Min Zeng, Jie Xie, Long Wang, Weiping Su, Yihe Hu
OBJECTIVE: To investigate the clinical effect of total knee arthroplasty on patients with knee osteoarthritis combined with mild to moderate valgus knee deformity.
 METHODS: A total of 15 patients received total knee arthroplasty for correcting mild (10°-15°) to moderate (15°-30°) valgus knee between January 2011 and February 2014 in Xiangya Hospital of Central South University. We adopted a stable prosthesis surgery through patellar medial approach, osteophytes cleaning, conventional osteotomy, a selective soft tissue release and balance technical correcting of knee valgus deformity...
September 28, 2016: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
Seung-Hyun Jung, Min Sung Kim, Chan Kwon Jung, Hyun-Chun Park, So Youn Kim, Jieying Liu, Ja-Seong Bae, Sung Hak Lee, Tae-Min Kim, Sug Hyung Lee, Yeun-Jun Chung
Follicular thyroid adenoma (FTA) precedes follicular thyroid carcinoma (FTC) by definition with a favorable prognosis compared to FTC. However, the genetic mechanism of FTA to FTC progression remains unknown. For this, it is required to disclose FTA and FTC genomes in mutational and evolutionary perspectives. We performed whole-exome sequencing and copy number profiling of 14 FTAs and 13 FTCs, which exhibited previously-known gene mutations (NRAS, HRAS, BRAF, TSHR and EIF1AX) and copy number alterations (CNAs) (22q loss and 1q gain) in follicular tumors...
September 9, 2016: Oncotarget
Mónica Franco, Vanda Nogueira
PURPOSE: We report on a case of unilateral acute syphilitic posterior placoid chorioretinitis (ASPPC) with spontaneous resolution of the lesions, and discuss the role of an altered versus adequate immune response as the major pathogenic factor. METHODS: We describe a case of acute loss of visual acuity (VA) in the left eye (LE) in a 55-year-old healthy man. RESULTS: The patient presented with VA of 20/20 in the right eye (RE) and hand movements in the LE...
2016: GMS Ophthalmol Cases
Marie-Louise Kampmann, Anders Buchard, Claus Børsting, Niels Morling
Here, we demonstrate that punches from buccal swab samples preserved on FTA cards can be used for high-throughput DNA sequencing, also known as massively parallel sequencing (MPS). We typed 44 reference samples with the HID-Ion AmpliSeq Identity Panel using washed 1.2 mm punches from FTA cards with buccal swabs and compared the results with those obtained with DNA extracted using the EZ1 DNA Investigator Kit. Concordant profiles were obtained for all samples. Our protocol includes simple punch, wash, and PCR steps, reducing cost and hands-on time in the laboratory...
2016: BioTechniques
Quan-Jiang Song, Xiao-Gang Weng, Dong-Jie Cai, Wang Zhang, Jiu-Feng Wang
Bovine viral diarrhea virus (BVDV), the causative agent of bovine viral diarrhea/mucosal disease (BVD/MD), is an important pathogen of cattle and other wild animals throughout the world. BVDV infection typically leads to an impaired immune response in cattle. In the present study, we investigated the effect of Forsythoside A (FTA) on BVDV infection of bovine peripheral blood mononuclear cells (PBMCs). We found that Forsythoside A could not only promote proliferation of PBMCs and T cells activation but also inhibit the replication of BVDV as well as apoptosis induced by BVDV...
2016: PloS One
Roberta Duarte Bezerra Pinto, Thiago Rubim Bellott Batista Nascimento, Jhéssica Pacheco Bicalho de Andrade, Fernanda Policarpo, Rogério Ribeiro Estrella, Enoi Guedes Vilar
Syphilis is an infectious disease with mucocutaneous lesions that are characteristic of their stage (primary, secondary, or tertiary). These are not always typical and may have other morphologies and unusual symptoms, making diagnosis difficult. The report herein is of a healthy woman with vesicular lesions associated with intense itching.  The diagnosis of secondary syphilis was confirmed with VDRL 1/512 and FTA Abs positive.  Histopathology showed spongiosis in the epidermis and infiltrated lymphocytes and plasma cell in the dermis...
2016: Dermatology Online Journal
Ria R Ghai, Mary I Thurber, Azza El Bakry, Colin A Chapman, Tony L Goldberg
BACKGROUND: Health clinics in rural Africa are typically resource-limited. As a result, many patients presenting with fever are treated with anti-malarial drugs based only on clinical presentation. This is a considerable issue in Uganda, where malaria is routinely over-diagnosed and over-treated, constituting a wastage of resources and an elevated risk of mortality in wrongly diagnosed patients. However, rapid diagnostic tests (RDTs) for malaria are increasingly being used in health facilities...
2016: Malaria Journal
Naiyu Zheng, Jianing Zeng, Qin C Ji, Aida Angeles, Anne-Francoise Aubry, Shenita Basdeo, Adela Buzescu, Ishani Savant Landry, Navin Jariwala, Wesley Turley, Richard Burrell, Mark E Arnold
Dried saliva spot (DSS) sampling is a non-invasive sample collection technique for bioanalysis that can be potentially implemented at the patient's home. A UHPLC-MS/MS assay was developed using detergent-assisted sample extraction to quantify BMS-927711, a drug candidate in development for the treatment of migraines, in human DSS. By implementing DSS sampling at the patients' home, the bioanalytical sample collection for pharmacokinetic evaluation can be done at the time of the acute migraine attack without the need for clinical visits...
August 31, 2016: Analytica Chimica Acta
Sasiwimon Chumwangwapee, Artit Chingsungnoen, Sineenat Siri
In forensic DNA analyses, biological specimens are collected and stored for subsequent recovery and analysis of DNA. A cost-effective and efficient DNA recovery approach is therefore a need. This study aims to produce a plasma modified cellulose-chitosan membrane (pCE-CS) that efficiently binds and retains DNA as a potential DNA collecting card. The pCE-CS membrane was produced by a phase separation of ionic liquid dissolving CE and CS in water with subsequent surface-modification by a two-step exposure of argon plasma and nitrogen gas...
July 30, 2016: Forensic Science International. Genetics
Zhentao Man, Dan Sha, Shui Sun, Tao Li, Bin Li, Guang Yang, Laibo Zhang, Changshun Wu, Peng Jiang, Xiaojuan Han, Wei Li
Total hip arthroplasty (THA) is a common procedure for the treatment of end-stage hip joint disease, and the demand for revision THA will double by 2026. Ti6Al4V (Titanium, 6% Aluminum, and 4% Vanadium) is a kind of alloy commonly used to make hip prothesis. To promote the osseointegration between the prothesis and host bone is very important for the revision THA. The peptide Arg-Gly-Asp (RGD) could increase cell attachment and has been used in the vascular tissue engineering. In this study, we combined the RGD with Ti6Al4V alloy using the covalent cross-linking method to fabricate the functional Ti6Al4V alloy (FTA)...
2016: BioMed Research International
María Carmen López de Las Hazas, Maria José Motilva, Carme Piñol, Alba Macià
In this study, a fast and simple blood sampling and sample pre-treatment method based on the use of the dried blood spot (DBS) cards and ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) for the quantification of olive oil phenolic metabolites in human blood was developed and validated. After validation, the method was applied to determine hydroxytyrosol metabolites in human blood samples after the acute intake of an olive oil phenolic extract. Using the FTA DMPK-A DBS card under optimum conditions, with 20µL as the blood solution volume, 100µL of methanol/Milli-Q water (50/50, v/v) as the extraction solvent and 7 disks punched out from the card, the main hydroxytyrosol metabolites (hydroxytyrosol-3-O-sulphate and hydroxytyrosol acetate sulphate) were identified and quantified...
October 1, 2016: Talanta
Yasushi Akamatsu, Hideo Kobayashi, Yoshihiro Kusayama, Ken Kumagai, Tomoyuki Saito
PURPOSE: To show the differences in lateral and anterior femoral shaft bowing (FSB) between radiographic and computed tomography (CT) images and to assess whether FSB is associated with various predictive factors in women with knee osteoarthritis (OA) using CT images. METHODS: We enrolled 135 Japanese women with medial compartment knee OA. Lateral and anterior FSB were measured by radiography and reconstructed CT. Age, body mass index (BMI), femorotibial angle (FTA), femoral length, and lumbar spine and femoral neck bone mineral density (BMD) were set as predictive factors for progression of knee OA...
September 2016: Archives of Orthopaedic and Trauma Surgery
Elise Daudens-Vaysse, Martine Ledrans, Noellie Gay, Vanessa Ardillon, Sylvie Cassadou, Fatiha Najioullah, Isabelle Leparc-Goffart, Dominique Rousset, Cécile Herrmann, Raymond Cesaire, Marianne Maquart, Olivier Flusin, Séverine Matheus, Patricia Huc-Anaïs, Josiane Jaubert, Anne Criquet-Hayot, Bruno Hoen, Felix Djossou, Corinne Locatelli-Jouans, Alain Blateau, Anne-Marie McKenzie, Mathilde Melin, Patrick Saint-Martin, Frédérique Dorléans, Claudine Suivant, Luisiane Carvalho, Marion Petit-Sinturel, Audrey Andrieu, Harold Noël, Alexandra Septfons, Anne Gallay, Marie-Claire Paty, Laurent Filleul, André Cabié
Following of the emergence of Zika virus in Brazil in 2015, an epidemiological surveillance system was quickly implemented in the French overseas Territories of America (FTA) according to previous experience with dengue and chikungunya and has detected first cases of Zika. General practitioners and medical microbiologists were invited to report all clinically suspected cases of Zika, laboratory investigations were systematically conducted (RT-PCR). On 18 December, the first autochthonous case of Zika virus infection was confirmed by RT-PCR on French Guiana and Martinique, indicating introduction of Zika virus in FTA...
July 14, 2016: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
Hirotomo Kato, Eduardo A Gomez, Luiggi Martini-Robles, Jenny Muzzio, Lenin Velez, Manuel Calvopiña, Daniel Romero-Alvarez, Tatsuyuki Mimori, Hiroshi Uezato, Yoshihisa Hashiguchi
A countrywide epidemiological study was performed to elucidate the current geographic distribution of causative species of cutaneous leishmaniasis (CL) in Ecuador by using FTA card-spotted samples and smear slides as DNA sources. Putative Leishmania in 165 samples collected from patients with CL in 16 provinces of Ecuador were examined at the species level based on the cytochrome b gene sequence analysis. Of these, 125 samples were successfully identified as Leishmania (Viannia) guyanensis, L. (V.) braziliensis, L...
July 2016: PLoS Neglected Tropical Diseases
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