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Thitaporn Phetkaew, Marissak Kalpravidh, Rampaipat Penchome, Chalika Wangdee
OBJECTIVE:  This article aimed to determine and compare the angular values of the pelvic limb in normal and medial patellar luxation (MPL) stifles in Chihuahuas using radiography and computed tomographic (CT) scan, to identify the relationship between pelvic limb angles and severity of MPL. In addition, radiographic and CT images were compared to determine the more suitable method of limb deformity assessment. METHODS:  Sixty hindlimbs of Chihuahuas were divided into normal and grade 1, 2, 3 and 4 MPL groups...
February 2018: Veterinary and Comparative Orthopaedics and Traumatology: V.C.O.T
Fabia Wyss, Martin Schneiter, Udo Hetzel, Fta Pathologie, Saskia Keller, Martin Frenz, Jaroslav Rička, Jean-Michel Hatt
Pneumonia is a common complication of boid inclusion body disease (BIBD) in snakes. The tracheal mucociliary apparatus of eight boas ( Boa constrictor) and two pythons ( Python regius, Morelia viridis) was examined to assess whether absent or reduced mucociliary clearance could be a predisposing factor. Nine of the examined snakes were positive for BIBD by detection of inclusion bodies and three had lung pathologies other than the formation of inclusion bodies. A considerable individual variation of ciliary beat frequency (CBF, 3...
March 2018: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
Marie Sylvianne Rabodoarivelo, A Brandao, M C Cergole Novella, A G C Bombonatte, B Imperiale, N Rakotosamimanana, N Morcillo, V Rasolofo, J C Palomino, A Martin
Background: In low-income countries, rapid detection of tuberculosis (TB) drug resistance is often restricted by the difficulties of transporting and storing sputum samples from remote health centers to the reference laboratories where molecular tests are available. The aim of this study was to evaluate the performance of four transport and storage systems for molecular detection of rifampicin (RIF) and isoniazid (INH) resistance. Methods: This was a multicenter study...
January 2018: International Journal of Mycobacteriology
Xiao-Tian Zhang, Yue Ding, Ping Kang, Xin-Yu Zhang, Tong Zhang
Acute inflammation is a protective response of the host to physical injury and invading infection. Timely treatment of acute inflammatory reactions is essential to prevent damage to organisms that can eventually lead to chronic inflammation. Forsythoside A (FTA), an active constituent of Forsythia suspensa , has been reported to have anti-inflammatory, antioxidant, and antibacterial properties. Despite increasing knowledge of its anti-inflammatory effects, the mechanism and the effects on acute inflammation are poorly understood...
March 6, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Lan Yao, Zhen Xu, Hemiao Zhao, Zheng Tu, Zhifang Liu, Wanshui Li, Lan Hu, Lihua Wan
In this study, the complete mitochondrial genome (mtGenome) of six samples from three forensic cases was sequenced using the Ion Torrent Personal Genome Machine (PGM). The analyzed samples from forensic cases included bloodstains from several materials, such as gauze, Flinder's Technology Associates (FTA) cards and swabs. The age of the samples ranged from two months to twelve years. The complete mtGenomes were amplified using the tiling sequencing strategy which divided the whole mtGenome into 162 amplicons...
February 27, 2018: Legal Medicine
Gilda da Cunha Santos
CONTEXT: - Traditional methods for storing histologic and cytologic specimens for future use in molecular assays have consisted of either snap-freezing with cryopreservation or formalin-fixing, paraffin-embedding the samples. Although snap-freezing with cryopreservation is recommended for better preservation of nucleic acids, the infrastructure and space required for archiving impose challenges for high-volume pathology laboratories. Cost-effective, long-term storage at room temperature; relatively easy shipment; and standardized handling can be achieved with formalin-fixed, paraffin-embedded samples, but formalin fixation induces fragmentation and chemical modification of nucleic acids...
March 2018: Archives of Pathology & Laboratory Medicine
U B Inchara, R P Sathish, K M Shankar, P B Abhiman, P Prakash
A panel of four monoclonal antibodies (C-05, C-14, C-38 and C-56) specific to VP28 of White spot syndrome virus (WSSV) were evaluated individually and in cocktail to increase sensitivity of the Flow Through Assay (FTA) for detection of the virus. Recombinant VP28 and semi purified WSSV was used as antigen for evaluation. Out of the total 11 cocktails and four individual of MAbs, 2 MAb cocktails C-05 + C-56 and C-14 + C-56 exhibited highest sensitivity in the FTA. The two MAb cocktail were 100 times more sensitive than 1-step PCR and nearly equivalent to 2-step PCR for the detection of WSSV...
February 24, 2018: Journal of Immunological Methods
Vanessa R Melanson, Ryan Jochim, Michael Yarnell, Karen Bingham Ferlez, Soumya Shashikumar, Jason H Richardson
Background & objectives: Vector-borne pathogen surveillance programmes typically rely on the collection of large numbers of potential vectors followed by screening protocols focused on detecting pathogens in the arthropods. These processes are laborious, time consuming, expensive, and require screening of large numbers of samples. To streamline the surveillance process, increase sample throughput, and improve cost-effectiveness, a method to detect dengue virus and malaria parasites (Plasmodium falciparum) by leveraging the sugar-feeding behaviour of mosquitoes and their habit of expectorating infectious agents in their saliva during feeding was investigated in this study...
October 2017: Journal of Vector Borne Diseases
Hong-Lue Tan, Peng-Yi Dai, Wei-Feng Liu, Yan-Hao Yuan
OBJECTIVE: To explore the clinical efficacy of double-plate fixation for the treatment of old tibial plateau fractures with Schatzker type IV through anterior midline and posteromedial approaches. METHODS: From July 2013 to July 2015, 15 patients with old tibial plateau fractures were treated with internal fixation using locking reconstructive plate for the posteromedial fragment and anatomical locking plate for anteromedial fragment through antero midline and posteromedial approaches...
October 25, 2017: Zhongguo Gu Shang, China Journal of Orthopaedics and Traumatology
F Longo, T Nicetto, T Banzato, G Savio, M Drigo, R Meneghello, G Concheri, M Isola
The aim of this ex vivo study was to test a novel three-dimensional (3D) automated computer-aided design (CAD) method (aCAD) for the computation of femoral angles in dogs from 3D reconstructions of computed tomography (CT) images. The repeatability and reproducibility of three manual radiography, manual CT reconstructions and the aCAD method for the measurement of three femoral angles were evaluated: (1) anatomical lateral distal femoral angle (aLDFA); (2) femoral neck angle (FNA); and (3) femoral torsion angle (FTA)...
February 2018: Veterinary Journal
Hyun-Wook Kim, Ji-Han Kim, Jin-Kyu Seo, Derico Setyabrata, Yuan H Brad Kim
The aim of this study was to evaluate the effect of aging/freezing sequence and freezing rate on quality attributes and oxidative stability of frozen/thawed pork loins (M. longissimus lumborum, n = 6). Six treatments were prepared by combining 3 aging/freezing sequences (FT, freezing/thawing only; AFT, aging prior to freezing/thawing; and FTA, freezing/thawing and aging) with 2 freezing rates (slow- and fast-freezing). The lowest purge/thaw loss and drip loss were found for AFT, in which fast-freezing reduced total exudate loss (P < 0...
January 31, 2018: Meat Science
Stephan Braune, Christina König, Jason A Roberts, Axel Nierhaus, Oliver Steinmetz, Michael Baehr, Stefan Kluge, Claudia Langebrake
BACKGROUND: The aim of the study was to describe the population pharmacokinetics (PK) of meropenem in critically ill patients receiving sustained low-efficiency dialysis (SLED). METHODS: Prospective population PK study on 19 septic patients treated with meropenem and receiving SLED for acute kidney injury. Serial blood samples for determination of meropenem concentrations were taken before, during and after SLED in up to three sessions per patient. Nonparametric population PK analysis with Monte Carlo simulations were used...
January 30, 2018: Critical Care: the Official Journal of the Critical Care Forum
Muhammad Adeel Hassan, Junlong Liu, Muhammad Sohail Sajid, Altaf Mahmood, Shuai Yang Zhao, Qamar Abbas, Guiquan Guan, Hong Yin, Jianxun Luo
Piroplasmosis is one of the most important diseases of livestock, constraining optimal production and leading to economic loss. This study was carried out to detect Theileria annulata using 2 different molecular techniques: recombinase polymerase amplification (RPA) and conventional PCR. Blood samples were collected from 274 ticks infesting asymptomatic cattle from several counties of Chakwal, Faisalabad, and Jhang districts of Punjab province of Pakistan using FTA cards. Following extraction of genomic DNA, each sample was subjected to RPA optimized to amplify a 281 bp fragment of the Enolase gene for T...
January 26, 2018: Journal of Parasitology
Deanna D Rumble, Christopher P Hurt, David A Brown
BACKGROUND: Step kinematic variability has been characterized during gait using spatial and temporal kinematic characteristics. However, people can adopt different trajectory paths both between individuals and even within individuals at different speeds. Single point measures such as minimum toe clearance (MTC) and step length (SL) do not necessarily account for the multiple paths that the foot may take during the swing phase to reach the same foot fall endpoint. The purpose of this study was to test a step-by-step foot trajectory area (SBS-FTA) variability measure that is able to characterize sagittal plane foot trajectories of varying areas, and compare this measure against MTC and SL variability at different speeds...
2018: PloS One
Zhan-Feng Zhang, Dan Wang, Ji-Kang Min
OBJECTIVE: To study the correlation of postoperative femorotibial angle with medial compartmental joint line elevation after unicompartmental arthroplasty(UKA), as well as the correlation of joint line elevation with the clinical function by measuring radiological joint line. METHODS: A retrospective study of 56 patients from July 2012 to August 2015 was performed. The mean body mass index (BMI) was 23.5 (ranged, 18.3 to 30.1). The standing anteroposterior radiographs of these patients were assessed both pre-and post-operatively, and the knee function was evaluated according to HSS grading...
April 25, 2017: Zhongguo Gu Shang, China Journal of Orthopaedics and Traumatology
Marina Papaiakovou, Nils Pilotte, Ben Baumer, Jessica Grant, Kristjana Asbjornsdottir, Fabian Schaer, Yan Hu, Raffi Aroian, Judd Walson, Steven A Williams
BACKGROUND: Proper collection and storage of fecal samples is necessary to guarantee the subsequent reliability of DNA-based soil-transmitted helminth diagnostic procedures. Previous research has examined various methods to preserve fecal samples for subsequent microscopic analysis or for subsequent determination of overall DNA yields obtained following DNA extraction. However, only limited research has focused on the preservation of soil-transmitted helminth DNA in stool samples stored at ambient temperature or maintained in a cold chain for extended periods of time...
January 2018: PLoS Neglected Tropical Diseases
Jiali Zhang, Yue Zhang, Hailong Huang, Haiwei Zhang, Wei Lu, Guoqun Fu, Yunzhang Zhu
Forsythoside A (FTA), the major bioactive component extracted from Forsythiae fructus, has multiple biological properties especially anti-inflammatory property. Staphylococcus aureus (S. aureus), a Gram-positive organism, is one of most common pathogens that cause bovine mastitis. This study evaluated the anti-inflammatory effect of FTA in S. aureus-stimulated primary bovine mammary epithelial cells (bMEC). Primary bovine mammary epithelial cells were isolated from the mammary tissue of lactating cows and identified as bMEC...
January 9, 2018: Microbial Pathogenesis
Samira Mohammadi, Bahram Nasr Esfahani, Sharareh Moghim, Hossein Mirhendi, Fatemeh Riyahi Zaniani, Hajieh Ghasemian Safaei, Hossein Fazeli, Mahshid Salehi
Background: Nontuberculous mycobacteria (NTM) are a group of opportunistic pathogens and these are widely dispersed in water and soil resources. Identification of mycobacteria isolates by conventional methods including biochemical tests, growth rates, colony pigmentation, and presence of acid-fast bacilli is widely used, but these methods are time-consuming, labor-intensive, and may sometimes remain inconclusive. Materials and Methods: The DNA was extracted from NTM cultures using CTAB, Chelex, Chelex + Nonidet P-40, FTA® Elute card, and boiling The quantity and quality of the DNA extracted via these methods were determined using UV-photometer at 260 and 280 nm, and polymerase chain reaction (PCR) amplification of the heat-shock protein 65 gene with serially diluted DNA samples...
2017: Advanced Biomedical Research
Daisuke Abe, Satoshi Hamai, Ken Okazaki, Masato Yoshimoto, Takashi Komatsu, Yasuharu Nakashima
INTRODUCTION: Severe cases of genu varum represent a major challenge in obtaining normal configuration of the proximal tibia and overall limb alignment. PRESENTATION OF CASE: We performed inverted V-shaped high tibial osteotomy (HTO) by using a locking plate for recurrent severe bilateral tibia vara in a 15-year-old female patient with Turner syndrome. Preoperative medial proximal tibial angle (MPTA) and standing femorotibial angle (FTA) of the right/left legs were 67°/69° and 197°/203°, respectively...
December 8, 2017: International Journal of Surgery Case Reports
Kay Neumann, Markus Cavalar, Achim Rody, Luisa Friemert, Daniel A Beyer
INTRODUCTION: Growing evidence shows a causal role of high-risk humane papillomavirus (HPV) infections in the development of head and neck cancer. A recent case report shows two patients suffering from tonsillar cancer without any risk factors apart from their work as gynecologists doing laser ablations and loop electrosurgical excision procedures (LEEP). The aim of the present investigation is to evaluate whether surgical plume resulting from routine LEEPs of HSIL of the cervix uteri might be contaminated with the DNA of high-risk HPV...
December 13, 2017: Archives of Gynecology and Obstetrics
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