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Decontamination of probes

Frank Schwarz, Andrea Schmucker, Jürgen Becker
In patients with peri-implant mucositis and peri-implantitis, what is the efficacy of nonsurgical (i.e. referring to peri-implant mucositis and peri-implantitis) and surgical (i.e. referring to peri-implantitis) treatments with alternative or adjunctive measures on changing signs of inflammation compared with conventional nonsurgical (i.e. mechanical/ultrasonic debridement) and surgical (i.e. open flap debridement) treatments alone? After electronic database and hand search, a total of 40 publications (reporting on 32 studies) were finally considered for the qualitative and quantitative assessment...
December 2015: Int J Implant Dent
Ida Pam Ombura, Noel Onyango, Susan Odera, Florence Mutua, Joshua Nyagol
BACKGROUND: Although prevention and control of spread of multi-drug resistant tuberculosis strains is a global challenge, there is paucity of data on the prevalence of DR-TB in patients diagnosed with TB in referral hospitals in Kenya. The present study assessed patients' characteristics and prevalence of drug resistant TB in sputa smear positive TB patients presenting to Coast Provincial General Hospital (CPGH) in Mombasa, Kenya. METHODS: Drug resistance was evaluated in 258 randomly selected sputa smear TB positive cases between the periods of November 2011 to February 2012 at the CPGH-Mombasa...
2016: PloS One
Daseul Park, Hafiz Muhammad Shahbaz, Sun-Hyoung Kim, Mijin Lee, Wooseong Lee, Jong-Won Oh, Dong-Un Lee, Jiyong Park
Human norovirus (HuNoV) is the primary cause of viral gastroenteritis worldwide. Fresh blueberries are among high risk foods associated with norovirus related outbreaks. Therefore, it is important to assess intervention strategies to reduce the risk of foodborne illness. The disinfection efficiency of decontamination methods is difficult to evaluate for fruits and vegetables due to an inconsistent degree of contamination and irregular surface characteristics. The inactivation efficiency and mechanism of murine norovirus 1 (MNV-1, a surrogate for HuNoV) was studied on an experimentally prepared solidified agar matrix (SAM) to simulate blueberries using different wavelengths (A, B, C) of UV light both with and without TiO2 photocatalysis (TP)...
December 5, 2016: International Journal of Food Microbiology
Ben Buse, Stuart Kearns, Charles Clapham, Donovan Hawley
A prototype Peltier thermoelectric cooling unit has been constructed to cool a cold finger on an electron microprobe. The Peltier unit was tested at 15 and 96 W, achieving cold finger temperatures of -10 and -27°C, respectively. The Peltier unit did not adversely affect the analytical stability of the instrument. Heat conduction between the Peltier unit mounted outside the vacuum and the cold finger was found to be very efficient. Under Peltier cooling, the vacuum improvement associated with water vapor deposition was not achieved; this has the advantage of avoiding severe degradation of the vacuum observed when warming up a cold finger from liquid nitrogen (LN2) temperatures...
October 5, 2016: Microscopy and Microanalysis
Larine Kupski, Marisa Freitas, Daniela Ribeiro, Eliana Badiale Furlong, Eduarda Fernandes
Ochratoxin A (OTA) is a mycotoxin produced by several species of fungi from the Aspergillus and Penicillium genera that frequently grow in improperly stored food products. OTA has carcinogenic, teratogenic and nephrotoxic potential and sustains a high half-life in human blood. Despite the recently efforts to decontaminate OTA through its conversion into its metabolite ochratoxin alpha (OTα), there are just a few reports in literature comparing the toxic effects of these toxins. Thus, herein we studied and compared the proinflammatory and toxicological effects of OTA and its metabolite OTα in human neutrophils in vitro...
August 10, 2016: Toxicology
J Dubeau, B E Heinmiller, M Corrigan
There is the possibility for a worker at a nuclear installation, such as a nuclear power reactor, a fuel production facility or a medical facility, to come in contact with radioactive contaminants. When such an event occurs, the first order of business is to care for the worker by promptly initiating a decontamination process. Usually, the radiation protection personnel performs a G-M pancake probe measurement of the contamination in situ and collects part or all of the radioactive contamination for further laboratory analysis...
July 29, 2016: Radiation Protection Dosimetry
G Caccianiga, G Rey, A Paiusco, D Lauritano, F Cura, Z Ormianer, F Carinci
In periodontology, lasers have been suggested for the photodynamic therapy (PDT). Such therapy can be defined as the inactivation of cells, microorganisms or molecules induced by light and not by heat. The aim of our study is to assess the effect of Oxygen high-level laser therapy (OHLLT) in removing all bacterial deposits on root or implant surface by means of mechanical instrumentation and laser irradiation. OHLLT has two effects on targeted bacteria and tissues, decontamination and biostimulation. A total of 33 patients were randomly selected with a diagnosis of chronic periodontitis...
April 2016: Journal of Biological Regulators and Homeostatic Agents
Nídia Cristina Castro Dos Santos, Naira Maria Rebelatto Bechara Andere, Cássia Fernandes Araujo, Andrea Carvalho de Marco, Lúcio Murilo Dos Santos, Maria Aparecida Neves Jardini, Mauro Pedrine Santamaria
Diabetes has become a global epidemic. Its complications can have a significant impact on quality of life, longevity, and public health costs. The presence of diabetes might impair the prognosis of periodontal treatments due to its negative influence on wound healing. Antimicrobial photodynamic therapy (aPDT) is a local approach that can promote bacterial decontamination in periodontal pockets. The aim of this study was to investigate the local effect of adjunct aPDT to ultrasonic periodontal debridement (UPD) and compare it to UD only for the treatment of chronic periodontitis in type 2 diabetic patients...
November 2016: Lasers in Medical Science
Yoichi Taniguchi, Akira Aoki, Kazuto Sakai, Koji Mizutani, Walter Meinzer, Yuichi Izumi
The objective of this study was to evaluate an Er:YAG laser (ErL) application for periodontal regenerative surgery in angular bone defects at nine sites in six patients. Debridement was thoroughly performed using a combination of curettage with a Gracey-type curette and ErL irradiation at a panel setting of 70 mJ/pulse and 20 Hz with sterile saline spray. After applying an enamel matrix derivative and autogenous bone grafting, ErL was used to form a blood clot coagulation on the grafted bone surface at 50 mJ/pulse and 20 Hz without water spray for approximately 30 seconds...
July 2016: International Journal of Periodontics & Restorative Dentistry
I V Ioffe, V V Lesnoy
The results of treatment of 65 patients, suffering diffuse peritonitis, were analyzed. For the abdominal cavity sanation and intestinal decontamination the adopted selective bacteriophages (polyvalent pyobacteriophage, intesti-bacteriophage, and the coliproteus one) were applied. The abdominal cavity state was estimated while doing a programmed relaparotomy. Bacteriologic investigation of exudate, excreted along nasointestinal probe, and of peritoneal exudate was conducted. The intestinal motor-evacuation function restoration was estimated in accordance to data of ultrasonographic investigation...
February 2016: Klinichna Khirurhiia
Stuart J Froum, Alex S Dagba, Ye Shi, Alejandro Perez-Asenjo, Paul S Rosen, Wendy C W Wang
BACKGROUND: The aim was to identify and evaluate those surgical protocols reporting on positive clinical outcomes for treating peri-implantitis with 12 or more months of follow-up. Method of surface decontamination (SDC) was evaluated for any correlation with outcomes. RESULTS: A literature search was performed of all articles published in English between January 1, 2001 and April 30, 2015. Of the 639 identified, 26 satisfied the inclusion criteria. Outcomes reported on included reductions in bleeding on probing (BoP) and probing depth (PD), mean radiographic bone fill (RBF), and mean change in marginal soft tissue levels (MR±)...
June 2016: Implant Dentistry
Kachapilly Arun Jose, Majo Ambooken, Jayan Jacob Mathew, Annie Valayil Issac, Ajithkumar Parachalil Kunju, Renjith Athirkandathil Parameshwaran
INTRODUCTION: The use of adjuncts like chlorhexidine local delivery and diode laser decontamination have been found to improve the clinical outcomes of scaling and root planing in non-surgical periodontal therapy in patients with chronic periodontitis. AIM: To evaluate the effects of diode laser and chlorhexidine chip as adjuncts to scaling and root planing in the management of chronic periodontitis. The objective is to evaluate the outcome of chlorhexidine chip and diode laser as adjuncts to scaling and root planing on clinical parameters like Plaque Index, Gingival Index, probing pocket depth and clinical attachment level...
April 2016: Journal of Clinical and Diagnostic Research: JCDR
K M Roy, S Ahmed, T Inkster, A Smith, G Penrice
BACKGROUND: A case of Crimean-Congo haemorrhagic fever (CCHF) was imported into Scotland in 2012. AIM: To discuss the public health response to the case, and the control measures taken to prevent secondary transmission. METHODS: Following confirmation of the case, an incident management team (IMT) was convened to ensure that: (i) all individuals exposed to the case and/or their blood/body fluids were identified, assessed and followed-up appropriately; and (ii) the appropriate disinfection or disposal of equipment was used to manage the patient, laboratory specimens obtained from the case, and their environment...
July 2016: Journal of Hospital Infection
Sarmed S Sami, Krish Ragunath, Emilie A Wilkes, Martin James, Rodrigo Mansilla-Vivar, Jacobo Ortiz-Fernández-Sordo', Jonathan White, Amardeep Khanna, Marina Coletta, Sunil Samuel, Guruprasad P Aithal, Indra Neil Guha
BACKGROUND AND AIMS: Screening for oesophageal varices (OV) using conventional oesophagogastroduodenoscopy (C-OGD) is invasive and requires costly monitoring, recovery, and decontamination facilities. We aimed to evaluate the technical feasibility, acceptability and accuracy of a novel, portable, and disposable office-based transnasal endoscope (EG Scan(™) ) compared to C-OGD as the reference standard. METHODS: This was a prospective cohort study. Consecutive adult patients with liver cirrhosis were invited to participate...
April 28, 2016: Liver International: Official Journal of the International Association for the Study of the Liver
I Magiopoulos, J S McQuillan, C L Burd, M Mowlem, M-N Tsaloglou
Direct measurement and sampling of pristine environments, such as subglacial lakes, without introducing contaminating microorganisms and biomolecules from the surface, represents a significant engineering and microbiological challenge. In this study, we compare methods for decontamination of titanium grade 5 surfaces, the material extensively used to construct a custom-made probe for reaching, measuring and sampling subglacial Lake Ellsworth in West Antarctica. Coupons of titanium were artificially contaminated with Pseudomonas fluorescens bacteria and then exposed to a number of decontamination procedures...
April 2016: Journal of Microbiological Methods
Mario Roccuzzo, Luigi Gaudioso, Marco Lungo, Paola Dalmasso
AIM: To evaluate the efficacy of a reconstructive surgical procedure in single peri-implantitis infrabony defects. METHODS: Seventy-five patients with one peri-implantitis crater-like lesion with pocket depth (PD) ≥ 6 mm, were included. Each defect was assigned to one characteristic class, by an independent examiner. After implant decontamination, defects were filled with deproteinized bovine bone mineral with 10% collagen. RESULTS: At 1-year follow-up, four patients were lost and six implants removed...
March 2016: Journal of Clinical Periodontology
S Czurda, S Smelik, S Preuner-Stix, F Nogueira, T Lion
Nucleic acid amplification techniques permitting sensitive and rapid screening in patients at risk for invasive fungal infections are an important addition to conventional fungal diagnostic methods. However, contamination with fungal DNA may be a serious threat to the validity of fungal amplification-based assays. Besides rigorous handling procedures to avoid false-positive test results from exogenous sources, we have implemented protocols for comprehensive assessment of fungal contamination in all materials involved in the analytical process...
January 2016: Journal of Clinical Microbiology
Stuart J Froum, Scott H Froum, Paul S Rosen
This article presents the results of a consecutive case series of 170 treated peri-implantitis-affected implants in 100 patients with follow-up measurements from 2 to 10 years. A total of 51 implants in 38 patients previously reported on were followed for an additional 2.5 years, and 119 additional implants in 62 additional patients were treated with the same protocol and monitored for at least 2 years posttreatment. The treatment consisted of flap reflection, surface decontamination, use of enamel matrix derivative (EMD) or platelet-derived growth factor (PDGF), and guided bone regeneration with mineralized freezedried bone and/or anorganic bovine bone combined with PDGF or EMD and covered with an absorbable membrane and/or subepithelial connective tissue graft...
November 2015: International Journal of Periodontics & Restorative Dentistry
Pieter M M De Kesel, Willy E Lambert, Christophe P Stove
Caffeine is the probe drug of choice to assess the phenotype of the drug metabolizing enzyme CYP1A2. Typically, molar concentration ratios of paraxanthine, caffeine's major metabolite, to its precursor are determined in plasma following administration of a caffeine test dose. The aim of this study was to develop and validate an LC-MS/MS method for the determination of caffeine and paraxanthine in hair. The different steps of a hair extraction procedure were thoroughly optimized. Following a three-step decontamination procedure, caffeine and paraxanthine were extracted from 20 mg of ground hair using a solution of protease type VIII in Tris buffer (pH 7...
November 1, 2015: Talanta
K Jepsen, S Jepsen, M L Laine, D Anssari Moin, A Pilloni, B Zeza, M Sanz, A Ortiz-Vigon, A M Roos-Jansåker, S Renvert
There is a paucity of data for the effectiveness of reconstructive procedures in the treatment of peri-implantitis. The objective of this study was to compare reconstruction of peri-implant osseous defects with open flap debridement (OFD) plus porous titanium granules (PTGs) compared with OFD alone. Sixty-three patients (36 female, 27 male; mean age 58.4 y [SD 12.3]), contributing one circumferential peri-implant intraosseous defect, were included in a multinational, multicenter randomized trial using a parallel-group design...
January 2016: Journal of Dental Research
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