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https://www.readbyqxmd.com/read/28709491/band-pattern-analysis-of-mutations-in-rifampicin-resistance-strain-of-mycobacterium-tuberculosis-by-line-probe-assay-in-patients-from-delhi-india
#1
Himanshu Vashistha, M Hanif, K K Chopra, Ashwani Khanna, Divya Shrivastava
BACKGROUND: The GenoType MTBDRplus, a commercial Line Probe Assay (LPA) kit from Hain Lifescience, Germany, is endorsed by India's RNTCP Program for diagnosis of DRTB cases among smear-positive sputum samples. Although the LPA has been studied in several laboratories, there is a wide variation in existing M. tuberculosis strains across the globe, and false results can occur due to the presence of unique genetic mutations in different settings. AIM AND OBJECTIVE: An attempt was made to carry out band pattern analysis using LPA and also to observe uncommon mutations in MDR strains...
July 2017: Indian Journal of Tuberculosis
https://www.readbyqxmd.com/read/28671824/exclusive-extracellular-bioreduction-of-methyl-orange-by-azo-reductase-free-geobacter-sulfurreducens
#2
Yi-Nan Liu, Feng Zhang, Jie Li, Dao-Bo Li, Dong-Feng Liu, Wen-Wei Li, Han-Qing Yu
Azo dyes are a class of recalcitrant organic pollutants causing severe environmental pollution. For their biodecolorization, the azo reductase system was considered as the major molecular basis in bacteria. However, the intracellular localization of azo reductase limits their function for efficient azo dye decolorization. This limitation may be circumvented by electrochemically active bacteria (EAB) which is capable of extracellular respiration. To verify the essential role of extracellular respiration in azo dye decolorization, Geobacter sulfurreducens PCA, a model EAB, was used for the bioreduction of methyl orange (MO), a typical azo dye...
July 14, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28357001/laser-assisted-non-surgical-periodontal-therapy-a-double-blind-randomized-clinical-trial
#3
Joseph D Everett, Jeffrey A Rossmann, David G Kerns, Ibtisam Al-Hashimi
The objective of this study was to examine potential benefits of using laser therapy for secular decontamination in conjunction with scaling and root planing in the treatment of chronic periodontitis. The study was performed on 173 teeth in 14 patients in a split-mouth design, one side received scaling and root planing followed by laser therapy using a carbon dioxide (CO2) laser with an ablative handpiece (test group); the contralateral side received scaling and root planing without laser (control group). Clinical and laboratory parameters were evaluated prior to treatment and at 3 and 6 months following therapy; clinical measurements were performed by two blinded examiners...
2017: Open Dentistry Journal
https://www.readbyqxmd.com/read/28284251/bottom-up-or-top-down-unit-cost-estimation-of-tuberculosis-diagnostic-tests-in-india
#4
S Rupert, A Vassall, N Raizada, S D Khaparde, C Boehme, V S Salhotra, K S Sachdeva, S A Nair, A H Van't Hoog
SETTING: Of 18 sites that participated in an implementation study of the Xpert® MTB/RIF assay in India, we selected five microscopy centres and two reference laboratories. OBJECTIVE: To obtain unit costs of diagnostic tests for tuberculosis (TB) and drug-resistant TB. DESIGN: Laboratories were purposely selected to capture regional variations and different laboratory types. Both bottom-up and the top-down methods were used to estimate unit costs...
April 1, 2017: International Journal of Tuberculosis and Lung Disease
https://www.readbyqxmd.com/read/28101947/combined-surgical-therapy-of-advanced-peri-implantitis-evaluating-two-methods-of-surface-decontamination-a-7-year-follow-up-observation
#5
Frank Schwarz, Gordon John, Andrea Schmucker, Narja Sahm, Jürgen Becker
OBJECTIVES: To assess the long-term outcomes (>4 years) following combined surgical resective/regenerative therapy of advanced peri-implantitis lesions using two surface decontamination methods. MATERIAL & METHODS: Fifteen patients (n = 15 combined supra- and intrabony defects) completed a follow-up observation period of 7 years. The treatment procedure included access flap surgery, granulation tissue removal and implantoplasty at buccally and supracrestally exposed implant parts, and a randomly assigned decontamination of the unmodified intrabony implant surface areas using either (i) an Er:YAG laser (ERL) or (ii) plastic curettes + cotton pellets + sterile saline (CPS)...
March 2017: Journal of Clinical Periodontology
https://www.readbyqxmd.com/read/28038315/in-situ-probes-of-capture-and-decomposition-of-chemical-warfare-agent-simulants-by-zr-based-metal-organic-frameworks
#6
Anna M Plonka, Qi Wang, Wesley O Gordon, Alex Balboa, Diego Troya, Weiwei Guo, Conor H Sharp, Sanjaya D Senanayake, John R Morris, Craig L Hill, Anatoly I Frenkel
Zr-based metal organic frameworks (MOFs) have been recently shown to be among the fastest catalysts of nerve-agent hydrolysis in solution. We report a detailed study of the adsorption and decomposition of a nerve-agent simulant, dimethyl methylphosphonate (DMMP), on UiO-66, UiO-67, MOF-808, and NU-1000 using synchrotron-based X-ray powder diffraction, X-ray absorption, and infrared spectroscopy, which reveals key aspects of the reaction mechanism. The diffraction measurements indicate that all four MOFs adsorb DMMP (introduced at atmospheric pressures through a flow of helium or air) within the pore space...
January 18, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/27778309/infection-prevention-and-ultrasound-probe-decontamination-practices-in-europe-a-survey-of-the-european-society-of-radiology
#7
Christiane Marita Nyhsen, Hilary Humphreys, Carlos Nicolau, Gerhard Mostbeck, Michel Claudon
OBJECTIVES: Although ultrasound (US) is considered one of the safest imaging modalities, concerns have been raised regarding potential infection transmission risks through US procedures. A survey was undertaken by the European Society of Radiology (ESR) to establish infection prevention and control measures in US and to highlight the importance of good medical practice. METHODS: An online survey was sent to all 22,000 full ESR members. RESULTS: The response rate of completed surveys was 4...
December 2016: Insights Into Imaging
https://www.readbyqxmd.com/read/27747644/efficacy-of-alternative-or-adjunctive-measures-to-conventional-treatment-of-peri-implant-mucositis-and-peri-implantitis-a-systematic-review-and-meta-analysis
#8
REVIEW
Frank Schwarz, Andrea Schmucker, Jürgen Becker
In patients with peri-implant mucositis and peri-implantitis, what is the efficacy of nonsurgical (i.e. referring to peri-implant mucositis and peri-implantitis) and surgical (i.e. referring to peri-implantitis) treatments with alternative or adjunctive measures on changing signs of inflammation compared with conventional nonsurgical (i.e. mechanical/ultrasonic debridement) and surgical (i.e. open flap debridement) treatments alone? After electronic database and hand search, a total of 40 publications (reporting on 32 studies) were finally considered for the qualitative and quantitative assessment...
December 2015: International Journal of Implant Dentistry
https://www.readbyqxmd.com/read/27711122/prevalence-of-drug-resistance-mycobacterium-tuberculosis-among-patients-seen-in-coast-provincial-general-hospital-mombasa-kenya
#9
Ida Pam Ombura, Noel Onyango, Susan Odera, Florence Mutua, Joshua Nyagol
BACKGROUND: Although prevention and control of spread of multi-drug resistant tuberculosis strains is a global challenge, there is paucity of data on the prevalence of DR-TB in patients diagnosed with TB in referral hospitals in Kenya. The present study assessed patients' characteristics and prevalence of drug resistant TB in sputa smear positive TB patients presenting to Coast Provincial General Hospital (CPGH) in Mombasa, Kenya. METHODS: Drug resistance was evaluated in 258 randomly selected sputa smear TB positive cases between the periods of November 2011 to February 2012 at the CPGH-Mombasa...
2016: PloS One
https://www.readbyqxmd.com/read/27705845/inactivation-efficiency-and-mechanism-of-uv-tio2-photocatalysis-against-murine-norovirus-using-a-solidified-agar-matrix
#10
Daseul Park, Hafiz Muhammad Shahbaz, Sun-Hyoung Kim, Mijin Lee, Wooseong Lee, Jong-Won Oh, Dong-Un Lee, Jiyong Park
Human norovirus (HuNoV) is the primary cause of viral gastroenteritis worldwide. Fresh blueberries are among high risk foods associated with norovirus related outbreaks. Therefore, it is important to assess intervention strategies to reduce the risk of foodborne illness. The disinfection efficiency of decontamination methods is difficult to evaluate for fruits and vegetables due to an inconsistent degree of contamination and irregular surface characteristics. The inactivation efficiency and mechanism of murine norovirus 1 (MNV-1, a surrogate for HuNoV) was studied on an experimentally prepared solidified agar matrix (SAM) to simulate blueberries using different wavelengths (A, B, C) of UV light both with and without TiO2 photocatalysis (TP)...
December 5, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27702410/decontamination-in-the-electron-probe-microanalysis-with-a-peltier-cooled-cold-finger
#11
Ben Buse, Stuart Kearns, Charles Clapham, Donovan Hawley
A prototype Peltier thermoelectric cooling unit has been constructed to cool a cold finger on an electron microprobe. The Peltier unit was tested at 15 and 96 W, achieving cold finger temperatures of -10 and -27°C, respectively. The Peltier unit did not adversely affect the analytical stability of the instrument. Heat conduction between the Peltier unit mounted outside the vacuum and the cold finger was found to be very efficient. Under Peltier cooling, the vacuum improvement associated with water vapor deposition was not achieved; this has the advantage of avoiding severe degradation of the vacuum observed when warming up a cold finger from liquid nitrogen (LN2) temperatures...
October 5, 2016: Microscopy and Microanalysis
https://www.readbyqxmd.com/read/27597255/ochratoxin-a-activates-neutrophils-and-kills-these-cells-through-necrosis-an-effect-eliminated-through-its-conversion-into-ochratoxin-%C3%AE
#12
Larine Kupski, Marisa Freitas, Daniela Ribeiro, Eliana Badiale Furlong, Eduarda Fernandes
Ochratoxin A (OTA) is a mycotoxin produced by several species of fungi from the Aspergillus and Penicillium genera that frequently grow in improperly stored food products. OTA has carcinogenic, teratogenic and nephrotoxic potential and sustains a high half-life in human blood. Despite the recently efforts to decontaminate OTA through its conversion into its metabolite ochratoxin alpha (OTα), there are just a few reports in literature comparing the toxic effects of these toxins. Thus, herein we studied and compared the proinflammatory and toxicological effects of OTA and its metabolite OTα in human neutrophils in vitro...
August 10, 2016: Toxicology
https://www.readbyqxmd.com/read/27473702/multiple-methods-for-assessing-the-dose-to-skin-exposed-to-radioactive-contamination
#13
J Dubeau, B E Heinmiller, M Corrigan
There is the possibility for a worker at a nuclear installation, such as a nuclear power reactor, a fuel production facility or a medical facility, to come in contact with radioactive contaminants. When such an event occurs, the first order of business is to care for the worker by promptly initiating a decontamination process. Usually, the radiation protection personnel performs a G-M pancake probe measurement of the contamination in situ and collects part or all of the radioactive contamination for further laboratory analysis...
April 28, 2017: Radiation Protection Dosimetry
https://www.readbyqxmd.com/read/27469554/oxygen-high-level-laser-therapy-is-efficient-in-treatment-of-chronic-periodontitis-a-clinical-and-microbiological-study-using-pcr-analysis
#14
G Caccianiga, G Rey, A Paiusco, D Lauritano, F Cura, Z Ormianer, F Carinci
In periodontology, lasers have been suggested for the photodynamic therapy (PDT). Such therapy can be defined as the inactivation of cells, microorganisms or molecules induced by light and not by heat. The aim of our study is to assess the effect of Oxygen high-level laser therapy (OHLLT) in removing all bacterial deposits on root or implant surface by means of mechanical instrumentation and laser irradiation. OHLLT has two effects on targeted bacteria and tissues, decontamination and biostimulation. A total of 33 patients were randomly selected with a diagnosis of chronic periodontitis...
April 2016: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/27448223/local-adjunct-effect-of-antimicrobial-photodynamic-therapy-for-the-treatment-of-chronic-periodontitis-in-type-2-diabetics-split-mouth-double-blind-randomized-controlled-clinical-trial
#15
RANDOMIZED CONTROLLED TRIAL
Nídia Cristina Castro Dos Santos, Naira Maria Rebelatto Bechara Andere, Cássia Fernandes Araujo, Andrea Carvalho de Marco, Lúcio Murilo Dos Santos, Maria Aparecida Neves Jardini, Mauro Pedrine Santamaria
Diabetes has become a global epidemic. Its complications can have a significant impact on quality of life, longevity, and public health costs. The presence of diabetes might impair the prognosis of periodontal treatments due to its negative influence on wound healing. Antimicrobial photodynamic therapy (aPDT) is a local approach that can promote bacterial decontamination in periodontal pockets. The aim of this study was to investigate the local effect of adjunct aPDT to ultrasonic periodontal debridement (UPD) and compare it to UD only for the treatment of chronic periodontitis in type 2 diabetic patients...
November 2016: Lasers in Medical Science
https://www.readbyqxmd.com/read/27333008/a-novel-surgical-procedure-for-er-yag-laser-assisted-periodontal-regenerative-therapy-case-series
#16
Yoichi Taniguchi, Akira Aoki, Kazuto Sakai, Koji Mizutani, Walter Meinzer, Yuichi Izumi
The objective of this study was to evaluate an Er:YAG laser (ErL) application for periodontal regenerative surgery in angular bone defects at nine sites in six patients. Debridement was thoroughly performed using a combination of curettage with a Gracey-type curette and ErL irradiation at a panel setting of 70 mJ/pulse and 20 Hz with sterile saline spray. After applying an enamel matrix derivative and autogenous bone grafting, ErL was used to form a blood clot coagulation on the grafted bone surface at 50 mJ/pulse and 20 Hz without water spray for approximately 30 seconds...
July 2016: International Journal of Periodontics & Restorative Dentistry
https://www.readbyqxmd.com/read/27244910/-correction-of-enteral-insufficiency-syndrome-in-patients-suffering-diffuse-peritonitis
#17
I V Ioffe, V V Lesnoy
The results of treatment of 65 patients, suffering diffuse peritonitis, were analyzed. For the abdominal cavity sanation and intestinal decontamination the adopted selective bacteriophages (polyvalent pyobacteriophage, intesti-bacteriophage, and the coliproteus one) were applied. The abdominal cavity state was estimated while doing a programmed relaparotomy. Bacteriologic investigation of exudate, excreted along nasointestinal probe, and of peritoneal exudate was conducted. The intestinal motor-evacuation function restoration was estimated in accordance to data of ultrasonographic investigation...
February 2016: Klinichna Khirurhiia
https://www.readbyqxmd.com/read/27213530/successful-surgical-protocols-in-the-treatment-of-peri-implantitis-a-narrative-review-of-the-literature
#18
REVIEW
Stuart J Froum, Alex S Dagba, Ye Shi, Alejandro Perez-Asenjo, Paul S Rosen, Wendy C W Wang
BACKGROUND: The aim was to identify and evaluate those surgical protocols reporting on positive clinical outcomes for treating peri-implantitis with 12 or more months of follow-up. Method of surface decontamination (SDC) was evaluated for any correlation with outcomes. RESULTS: A literature search was performed of all articles published in English between January 1, 2001 and April 30, 2015. Of the 639 identified, 26 satisfied the inclusion criteria. Outcomes reported on included reductions in bleeding on probing (BoP) and probing depth (PD), mean radiographic bone fill (RBF), and mean change in marginal soft tissue levels (MR±)...
June 2016: Implant Dentistry
https://www.readbyqxmd.com/read/27190958/management-of-chronic-periodontitis-using-chlorhexidine-chip-and-diode-laser-a-clinical-study
#19
Kachapilly Arun Jose, Majo Ambooken, Jayan Jacob Mathew, Annie Valayil Issac, Ajithkumar Parachalil Kunju, Renjith Athirkandathil Parameshwaran
INTRODUCTION: The use of adjuncts like chlorhexidine local delivery and diode laser decontamination have been found to improve the clinical outcomes of scaling and root planing in non-surgical periodontal therapy in patients with chronic periodontitis. AIM: To evaluate the effects of diode laser and chlorhexidine chip as adjuncts to scaling and root planing in the management of chronic periodontitis. The objective is to evaluate the outcome of chlorhexidine chip and diode laser as adjuncts to scaling and root planing on clinical parameters like Plaque Index, Gingival Index, probing pocket depth and clinical attachment level...
April 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27174232/managing-the-risk-of-viral-haemorrhagic-fever-transmission-in-a-non-high-level-intensive-care-unit-experiences-from-a-case-of-crimean-congo-haemorrhagic-fever-in-scotland
#20
K M Roy, S Ahmed, T Inkster, A Smith, G Penrice
BACKGROUND: A case of Crimean-Congo haemorrhagic fever (CCHF) was imported into Scotland in 2012. AIM: To discuss the public health response to the case, and the control measures taken to prevent secondary transmission. METHODS: Following confirmation of the case, an incident management team (IMT) was convened to ensure that: (i) all individuals exposed to the case and/or their blood/body fluids were identified, assessed and followed-up appropriately; and (ii) the appropriate disinfection or disposal of equipment was used to manage the patient, laboratory specimens obtained from the case, and their environment...
July 2016: Journal of Hospital Infection
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