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https://www.readbyqxmd.com/read/27906436/iliac-crest-bone-graft-a-23-years-hystory-of-infection-at-donor-site-in-vertebral-arthrodesis-and-a-review-of-current-bone-substitutes
#1
L Babbi, G Barbanti-Brodano, A Gasbarrini, S Boriani
OBJECTIVE: This is an exemplary case report underlining a relevant morbidity which could be associated to the use of autologous iliac crest bone graft (ICBG) for spine fusion. CASE REPORT: Starting from 1990, a 25-years-old woman underwent two subsequent surgical treatments for non-Hodgkin lymphoma vertebral localizations. In the second surgery, arthrodesis was obtained with autograft through right posterior iliac crest osteotomy. During the chemotherapy treatment following the surgery, the patient suffered from infection at posterior iliac crest scar, the site of previous graft, caused by methicillin-resistant Staphylococcus aureus...
November 2016: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/27905624/-detection-of-the-meca-gene-in-coagulase-positive-staphylococcus-isolated-from-cats
#2
Nicolás Galarce, Loreto Muñoz, María Antonieta Jara, Paulo Lubí, Alejandra Sepúlveda, Sonia Anticevic
INTRODUCTION: Bacterial resistance is a global concern for public health. Reports of antimicrobial resistance, including that against methicillin, have increased in strains of coagulase positive Staphylococcus (CPS) isolated from pets, however in Chile this information is limited. OBJECTIVES: To determine the antimicrobial susceptibility profiles and to detect the mecA gene in CPS strains isolated from cats in Chile. MATERIALS AND METHODS: 134 samples were obtained from healthy cats and cats with skin lesions...
August 2016: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
https://www.readbyqxmd.com/read/27904402/microbiological-quality-and-variability-of-natural-microbiota-in-croatian-cheese-maturing-in-lambskin-sacks
#3
Jadranka Frece, Marija Vrdoljak, Mija Filipčić, Marko Jelić, Iva Čanak, Željko Jakopović, Jelka Pleadin, Ivana Gobin, Tibela Landeka Dragičević, Ksenija Markov
As in the traditional production of cheese in lambskin sacks raw cow's or sheep's milk is mostly used, the purpose of this study is to see how the production affects the microbiological quality of the cheese. To do that, we tested 39 samples of raw cow's and sheep's milk, curd, ripened cheese (15, 30 and 45 days) and lambskin sacks for native microbial population. Two-thirds of the milk, curd and cheese samples had higher counts of staphylococci and enterobacteria than permitted by regulations. Not a single sample had Salmonella and Listeria monocytogenes, but we did find Escherichia coli in sheep's milk and cheese, and yeast and mould in both types of milk and cheese...
June 2016: Food Technology and Biotechnology
https://www.readbyqxmd.com/read/27903603/multicenter-evaluation-of-a-modified-cefoxitin-disk-diffusion-method-and-pbp2a-testing-to-predict-meca-mediated-oxacillin-resistance-in-atypical-staphylococcus-aureus
#4
Shelley A Miller, James Karichu, Peggy Kohner, Nicolynn Cole, Janet A Hindler, Robin Patel, Sandra Richter, Romney M Humphries
Phenotypic variants of Staphylococcus aureus display small colony size, reduced pigment, hemolysis and/or coagulase activity are periodically isolated by the clinical laboratory. Antimicrobial susceptibility testing (AST) of these isolates is complicated as many do not grow on routine AST media, including Mueller Hinton agar and cation-adjusted Mueller Hinton broth. This multi-center study evaluated cefoxitin disk diffusion for 37 isolates (156 readings) of atypical S. aureus with MHA supplemented with 5% sheep's blood (BMHA), using mecA PCR as the reference standard...
November 30, 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27902784/dynamic-in-vivo-mutations-within-the-ica-operon-during-persistence-of-staphylococcus-aureus-in-the-airways-of-cystic-fibrosis-patients
#5
Bianca Schwartbeck, Johannes Birtel, Janina Treffon, Lars Langhanki, Alexander Mellmann, Devika Kale, Janina Kahl, Nina Hirschhausen, Claudia Neumann, Jean C Lee, Friedrich Götz, Holger Rohde, Hanae Henke, Peter Küster, Georg Peters, Barbara C Kahl
Cystic fibrosis (CF) is associated with chronic bacterial airway infections leading to lung insufficiency and decreased life expectancy. Staphylococcus aureus is one of the most prevalent pathogens isolated from the airways of CF patients. Mucoid colony morphology has been described for Pseudomonas aeruginosa, the most common pathogen in CF, but not for S. aureus. From the airways of 8 of 313 CF patients (2.5%) mucoid S. aureus isolates (n = 115) were cultured with a mean persistence of 29 months (range 1 month, 126 months)...
November 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27902777/the-two-component-system-arlrs-and-alterations-in-metabolism-enable-staphylococcus-aureus-to-resist-calprotectin-induced-manganese-starvation
#6
Jana N Radin, Jessica L Kelliher, Paola K Párraga Solórzano, Thomas E Kehl-Fie
During infection the host imposes manganese and zinc starvation on invading pathogens. Despite this, Staphylococcus aureus and other successful pathogens remain capable of causing devastating disease. However, how these invaders adapt to host-imposed metal starvation and overcome nutritional immunity remains unknown. We report that ArlRS, a global staphylococcal virulence regulator, enhances the ability of S. aureus to grow in the presence of the manganese-and zinc-binding innate immune effector calprotectin...
November 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27902490/detection-of-staphylococcus-aureus-in-cystic-fibrosis-patients-using-breath-voc-profiles
#7
A H Neerincx, B P Geurts, J van Loon, V Tiemes, J J Jansen, F J M Harren, L A J Kluijtmans, P J F M Merkus, S M Cristescu, L M C Buydens, R A Wevers
Staphylococcus aureus (S. aureus) is a common bacterium infecting children with cystic fibrosis (CF). Since current detection methods are difficult to perform in children, there is need for an alternative. This proof of concept study investigates whether breath profiles can discriminate between S. aureus infected and non-infected CF patients based on volatile organic compounds (VOCs). We collected exhaled breath of CF patients with and without S. aureus airways infections in which VOCs were identified using gas chromatography-mass spectrometry...
November 30, 2016: Journal of Breath Research
https://www.readbyqxmd.com/read/27902427/phenotypic-and-genetic-characterization-of-multi-drug-resistant-staphylococcus-aureus-in-the-tropics-of-southeast-asia
#8
Siti Norayuni Mohd Zulkeflle, Yus Amira Yusaimi, Norio Sugiura, Koji Iwamoto, Masafumi Goto, Motoo Utsumi, Nor'azizi Bin Othman, Zuriati Zakaria, Hirofumi Hara
Antibiotic resistance has become a major public health problem throughout the world. The presence of antibiotic resistant bacteria such as Staphylococcus aureus (S. aureus) and antibiotic resistance genes (ARGs) in hospital wastewater is cause for great concern today. In this study, 276 S. aureus isolates were recovered from hospital wastewater samples in Malaysia. All of the isolates were screened for susceptibility to nine different classes of antibiotics: ampicillin (AMP), ciprofloxacin (CFX), gentamicin (GMN), kanamycin (KMN), erythromycin (ERM), vancomycin (VAN), trimetoprim and sulfamethaxazole (TM-SMZ), chloramphenicol (CMP), tetracycline (TET), and nalidixic acid (NAL)...
November 3, 2016: Microbiology
https://www.readbyqxmd.com/read/27902406/mlvf-as-a-method-for-rapid-and-cost-effective-typing-of-animal-associated-staphylococcus-aureus-strains-from-lineages-other-than-st398
#9
Maja Kosecka-Strojek, Weronika M Ilczyszyn, Aneta Buda, Klaudia Polakowska, Krzysztof Murzyn, Tomasz Panz, Anna Bialecka, Andrzej Kasprowicz, Antoni Jakubczak, Jaroslaw Krol, Alina Wieliczko, Benedykt Wladyka, Jacek Miedzobrodzki
In veterinary medicine, Staphylococcus aureus is associated with a range of mild to severe infections. The high density of livestock in intensive farming systems increases the risk of disease spread and hampers its control and measures of prevention, making S. aureus one of the most important animal pathogens. Multiple-locus VNTR fingerprinting (MLVF) has been successfully applied to the characterization of livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) ST398 but not to the characterization of a wide range of other animal isolates...
October 25, 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27902394/validation-of-self-administered-nasal-swabs-and-postage-for-the-isolation-of-staphylococcus-aureus
#10
Ewan Harrison, Nicholas S Gleadall, Xiaoliang Ba, John Danesh, Sharon J Peacock, Mark Holmes
Staphylococcus aureus carriers are at higher risk of S. aureus infection and are a reservoir for transmission to others. Detection of nasal S. aureus carriage is important for both targeted decolonisation and epidemiological studies. Self-administered nasal swabbing has been reported previously, but the impact of posting swabs prior to culture on S. aureus yield has not been investigated. A longitudinal cohort study was performed in which healthy volunteers were recruited, trained in the swabbing procedure, and asked to take weekly nasal swabs for 6 weeks (median: 3 weeks, range 1 to 6 weeks)...
October 31, 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27902390/virulence-determinants-in-clinical-staphylococcus-aureus-from-monomicrobial-and-polymicrobial-infections-of-diabetic-foot-ulcers
#11
Kavitha Shettigar, Spoorthi Jain, Deepika V Bhat, Raviraj Acharya, Lingadakai Ramachandra, Kapaettu Satyamoorthy, T S Murali
Antibiotic resistance in Staphylococcus aureus is a major public health concern and Methicillin Resistant S. aureus has emerged as an important pathogen. We characterized S. aureus isolates from monomicrobial and polymicrobial wound infections from 200 diabetic individuals with foot ulcers to understand their underlying diversity and pathogenicity. Staphylococcal Cassette Chromosome mec typing was performed and genes coding for production of biofilm, Panton-Valentine Leukocidin, Toxic Shock Syndrome Toxin, Leukotoxins DE and M were screened...
October 19, 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27902380/antimicrobial-resistance-profiling-and-molecular-typing-of-methicillin-resistant-staphylococcus-aureus-mrsa-isolated-from-a-malaysian-teaching-hospital
#12
Ainihayati Noordin, Hassriana Fazzila Sapri, Nurul Azirah Mohamad Sani, Shih Kei Leong, Xin-Ee Tan, Toh Leong Tan, Noraziah Mohamad Zin, Hui-Min Neoh, Salasawati Hussin
The annual prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in Malaysia has been estimated to be at 30-40% of all S. aureus infections. Nevertheless, data on the antimicrobial resistance and genetic diversity of Malaysian MRSAs remain few. In 2009, we collected 318 MRSA strains from various wards of our teaching hospital located in Kuala Lumpur, the capital city of Malaysia, and performed antimicrobial susceptibility testing on the strains. The strains were then molecularly characterized via SCCmec and virulence gene (cna, sea, seb, sec, sed, see, seg, seh, sei, eta, etb, PVL and TSST-1) typing; a subset of 49 strains isolated from the Intensive Care Unit (ICU) were also typed using Pulsed-Field Gel Electrophoresis (PFGE)...
November 7, 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27902375/improving-time-to-optimal-staphylococcus-aureus-treatment-using-a-penicillin-binding-protein-2a-assay
#13
Sonia N Rao, Sheila K Wang, Jose Gonzalez Zamora, Amy P Hanson, Radhika S Polisetty, Kamaljit Singh
The penicillin-binding protein 2a (PBP2a) assay is a quick, accurate and inexpensive test for determining methicillin susceptibility in Staphylococcus aureus. A pre-post study design was conducted using a PBP2a assay with and without the impact of an antimicrobial stewardship (AS) intervention to improve time to optimal therapy for methicillin-susceptible (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) isolates. Our results demonstrate significantly improved time to optimal therapy and support the use of a PBP2a assay as part of an AS program for all healthcare facilities, especially those with limited resources...
October 14, 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27902367/staphylococcal-cassette-chromosome-mec-typing-and-meca-sequencing-in-methicillin-resistant-staphylococci-from-algeria-a-highly-diversified-element-with-new-mutations-in-meca
#14
Ferhat Djoudi, Celestino Bonura, Abdelaziz Touati, Aurora Aléo, Said Benallaoua, Caterina Mammina
Genetic mechanisms of methicillin resistance are still relevant in staphylococci. The aims of this study are to assess the possible exchanges of staphylococcal cassette chromosome mec (SCCmec) among isolates of methicillin-resistant staphylococci (MRS) and to check for known or new mutations in mecA DNA. A total of 35 MRS non-repetitive isolates were recovered, including 20 Staphylococcushaemolyticus, 7 Staphylococcusaureus, 4 Staphylococcussciuri, 2 Staphylococcussaprophyticus and 1 isolate each of Staphylococcusxylosus and Staphylococcuslentus...
November 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27901415/burden-of-surgical-site-infections-associated-with-select-spine-operations-and-involvement-of-staphylococcus-aureus
#15
Harshila Patel, Hanane Khoury, Douglas Girgenti, Sharon Welner, Holly Yu
BACKGROUND: Spine operations may be indicated for treatment of diseases including vertebral injuries, degenerative spinal conditions, disk disease, spinal misalignments, or malformations. Surgical site infection (SSI) is a clinically important complication of spine surgery. Staphylococcus aureus, including methicillin-resistant Staphylococcus aureus (MRSA), is a leading cause of post-spinal SSIs. METHODS: PubMed and applicable infectious disease conference proceedings were searched to identify relevant published studies...
November 30, 2016: Surgical Infections
https://www.readbyqxmd.com/read/27900629/dracorhodin-perochlorate-attenuates-staphylococcus-aureus-usa300-virulence-by-decreasing-%C3%AE-toxin-expression
#16
Yumin Liu, Dongxue Shi, Yan Guo, Meng Li, Yonghong Zha, Quankai Wang, Jianfeng Wang
α-Toxin, a pore-forming toxin secreted by most Staphylococcus aureus, plays critical role in the pathogenesis associated with various infectious diseases. The USA300 which is a major international epidemic methicilin-resisrant S. aureus has spread rapidly to multiple countries and become an emerging public health concern. In this study, the in vitro efficacy of Dracorhodin Perochlorate (DP) against USA300 virulence was evaluated. Using susceptibility testing, immunoblots, rabbit blood haemolytic assay and real-time RT-PCR, we observed that the α-toxin production was decreased when USA300 was co-cultured with different sub-inhibitory concentration of DP...
January 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27900434/combination-of-microbiological-spectroscopic-and-molecular-docking-techniques-to-study-the-antibacterial-mechanism-of-thymol-against-staphylococcus-aureus-membrane-damage-and-genomic-dna-binding
#17
Lang-Hong Wang, Zhi-Hong Zhang, Xin-An Zeng, De-Ming Gong, Man-Sheng Wang
Thymol (2-isopropyl-5-methylphenol) is a natural ingredient used as flavor or preservative agent in food products. The antibacterial mechanism of thymol against Gram-positive, Staphylococcus aureus was investigated in this work. A total of 15 membrane fatty acids were identified in S. aureus cells by gas chromatography-mass spectrometry. Exposure to thymol at low concentrations induced obvious alterations in membrane fatty acid composition, such as decreasing the proportion of branched 12-methyltetradecanoic acid and 14-methylhexadecanoic acid (from 22...
November 29, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27899152/the-anti-staphylococcus-aureus-activity-of-the-phenanthrene-fraction-from-fibrous-roots-of-bletilla-striata
#18
Jing-Jing Guo, Bin-Ling Dai, Ni-Pi Chen, Li-Xia Jin, Fu-Sheng Jiang, Zhi-Shan Ding, Chao-Dong Qian
BACKGROUND: Bletillae Rhizoma, the tuber of Bletilla striata, has been used in Chinese traditional medicine to treat infectious diseases. Chemical studies indicated that phenanthrene was one of the most important components of the herb, with a broad spectrum of antibiotic activity against Gram-positive bacteria. The objective of this study was to further characterize the antibacterial activity of the phenanthrene fraction from the fibrous root of the pseudobulb of B. striata. METHODS: The phenanthrene fraction (EF60) from the ethanol extract of fibrous roots of Bletilla striata pseudobulbs was isolated using polyamide column chromatography...
November 29, 2016: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/27899142/vancomycin-microspheres-reduce-postoperative-spine-infection-in-an-in-vivo-rabbit-model
#19
Gang Liu, Si Chen, Jun Fang, Baoshan Xu, Shuang Li, Yonghong Hao, Naif A Al-Dhabi, Shucai Deng, Veeramuthu Duraipandiyan
BACKGROUND: Surgical site infections are common and devastating complications after implants related surgeries. Staphylococcus aureus contamination is a leading cause of surgical site infections. This study aims at assessing the effect of vancomycin microspheres on reducing Staphylococcus aureus infection in an in vivo rabbit model. METHODS: Sixty surgical sites of 20 New Zealand White rabbits underwent spinal implant were randomly divided to three groups: the control group, the vancomycin group and vancomycin microspheres group...
November 29, 2016: BMC Pharmacology & Toxicology
https://www.readbyqxmd.com/read/27899074/antibiotic-resistance-and-clonal-diversity-of-invasive-staphylococcus-aureus-in-the-rural-ashanti-region-ghana
#20
Denise Dekker, Manuel Wolters, Eva Mertens, Kennedy Gyau Boahen, Ralf Krumkamp, Daniel Eibach, Norbert G Schwarz, Yaw Adu-Sarkodie, Holger Rohde, Martin Christner, Florian Marks, Nimako Sarpong, Jürgen May
BACKGROUND: Staphylococcus aureus is among the most common pathogens isolated from blood cultures in Ghana; yet the epidemiology of blood infections in rural settings is poorly described. This study aims to investigate antimicrobial susceptibility and clonal diversity of S. aureus causing bloodstream infections in two hospitals in the Ashanti Region, Ghana. METHODS: Blood cultures were performed for all febrile patients (≥37.5 °C) on hospital admission. Antibiotic susceptibility testing for S...
November 29, 2016: BMC Infectious Diseases
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