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E. coli Nissle

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https://www.readbyqxmd.com/read/28398304/engineered-probiotic-escherichia-coli-can-eliminate-and-prevent-pseudomonas-aeruginosa-gut-infection-in-animal-models
#1
In Young Hwang, Elvin Koh, Adison Wong, John C March, William E Bentley, Yung Seng Lee, Matthew Wook Chang
Bacteria can be genetically engineered to kill specific pathogens or inhibit their virulence. We previously developed a synthetic genetic system that allows a laboratory strain of Escherichia coli to sense and kill Pseudomonas aeruginosa in vitro. Here, we generate a modified version of the system, including a gene encoding an anti-biofilm enzyme, and use the probiotic strain Escherichia coli Nissle 1917 as host. The engineered probiotic shows in vivo prophylactic and therapeutic activity against P. aeruginosa during gut infection in two animal models (Caenorhabditis elegans and mice)...
April 11, 2017: Nature Communications
https://www.readbyqxmd.com/read/28337589/colonization-by-non-pathogenic-bacteria-alters-mrna-expression-of-cytochromes-p450-in-originally-germ-free-mice
#2
L Jourová, P Anzenbacher, B Lišková, Z Matušková, P Hermanová, T Hudcovic, H Kozáková, L Hrnčířová, E Anzenbacherová
Gut microbiota provides a wide range of beneficial function for the host and has an immense effect on the host's health state. It has also been shown that gut microbiome is often involved in the biotransformation of xenobiotics; however, the molecular mechanisms of the interaction between the gut bacteria and the metabolism of drugs by the host are still unclear. To investigate the effect of microbial colonization on messenger RNA (mRNA) expression of liver cytochromes P450 (CYPs), the main drug-metabolizing enzymes, we used germ-free (GF) mice, lacking the intestinal flora and mice monocolonized by non-pathogenic bacteria Lactobacillus plantarum (NIZO2877) or probiotic bacteria Escherichia coli Nissle 1917 compared to specific pathogen-free (SPF) mice...
March 23, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28172826/p115-escherichia-coli-nissle-1917-modulate-gut-microbiota-composition-in-ulcerative-colitis-patients
#3
C Graziani, V Petito, F Del Chierico, F Mangiola, S Pecere, E Schiavoni, M Pizzoferrato, L R Lopetuso, L Putignani, A Gasbarrini, F Scaldaferri
No abstract text is available yet for this article.
February 1, 2017: Journal of Crohn's & Colitis
https://www.readbyqxmd.com/read/28105081/cross-feeding-of-glucose-metabolism-byproducts-of-escherichia-coli-human-gut-isolates-and-probiotic-strains-affect-survival-of-vibrio-cholerae
#4
Chirantana Sengupta, Manjula Ekka, Saurabh Arora, Prashant D Dhaware, Rukhsana Chowdhury, Saumya Raychaudhuri
Vibrio cholerae converts glucose into either acid or the neutral end product acetoin and its survival in carbohydrate enriched media is linked to the nature of the byproducts produced. It has been demonstrated in this study that Escherichia coli strain isolated from the gut of healthy human volunteers and the commonly used probiotic E. coli Nissle strain that metabolize glucose to acidic byproducts drastically reduce the survival of V. cholerae strains irrespective of their glucose sensitivity and acetoin production status...
2017: Gut Pathogens
https://www.readbyqxmd.com/read/28094807/antimicrobial-probiotics-reduce-salmonella-enterica-in-turkey-gastrointestinal-tracts
#5
Brittany Forkus, Seth Ritter, Michail Vlysidis, Kathryn Geldart, Yiannis N Kaznessis
Despite the arsenal of technologies employed to control foodborne nontyphoidal Salmonella (NTS), infections have not declined in decades. Poultry is the primary source of NTS outbreaks, as well as the fastest growing meat sector worldwide. With recent FDA rules for phasing-out antibiotics in animal production, pressure is mounting to develop new pathogen reduction strategies. We report on a technology to reduce Salmonella enteritidis in poultry. We engineered probiotic E. coli Nissle 1917, to express and secrete the antimicrobial peptide, Microcin J25...
January 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28094590/effects-of-supernatants-from-escherichia-coli-nissle-1917-and-faecalibacterium-prausnitzii-on-intestinal-epithelial-cells-and-a-rat-model-of-5-fluorouracil-induced-mucositis
#6
Hanru Wang, Yoga D Jatmiko, Susan E P Bastian, Suzanne Mashtoub, Gordon S Howarth
Faecalibacterium prausnitzii (Fp) and Escherichia coli Nissle 1917 (EcN) are probiotics, which have been reported to ameliorate certain gastrointestinal disorders. We evaluated the effects of supernatants (SN) derived from Fp and EcN on 5-fluorouracil (5-FU)-treated intestinal cells and in a rat model of mucositis. In vitro: IEC-6, Caco-2, and T-84 cells were analyzed for viability and monolayer permeability. In vivo: Female dark agouti rats were gavaged with Fp or EcN SN and injected intraperitoneally with saline (control) or 5-FU to induce mucositis...
February 2017: Nutrition and Cancer
https://www.readbyqxmd.com/read/28069496/probiotic-e-coli-nissle-1917-biofilms-on-silicone-substrates-for-bacterial-interference-against-pathogen-colonization
#7
Quan Chen, Zhiling Zhu, Jun Wang, Analette I Lopez, Siheng Li, Amit Kumar, Fei Yu, Haoqing Chen, Chengzhi Cai, Lijuan Zhang
Bacterial interference is an alternative strategy to fight against device-associated bacterial infections. Pursuing this strategy, a non-pathogenic bacterial biofilm is used as a live, protective barrier to fence off pathogen colonization. In this work, biofilms formed by probiotic Escherichia coli strain Nissle 1917 (EcN) are investigated for their potential for long-term bacterial interference against infections associated with silicone-based urinary catheters and indwelling catheters used in the digestive system, such as feeding tubes and voice prostheses...
January 6, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28018313/outer-membrane-vesicles-and-soluble-factors-released-by-probiotic-escherichia-coli-nissle-1917-and-commensal-ecor63-enhance-barrier-function-by-regulating-expression-of-tight-junction-proteins-in-intestinal-epithelial-cells
#8
Carina-Shianya Alvarez, Josefa Badia, Manel Bosch, Rosa Giménez, Laura Baldomà
The gastrointestinal epithelial layer forms a physical and biochemical barrier that maintains the segregation between host and intestinal microbiota. The integrity of this barrier is critical in maintaining homeostasis in the body and its dysfunction is linked to a variety of illnesses, especially inflammatory bowel disease. Gut microbes, and particularly probiotic bacteria, modulate the barrier integrity by reducing gut permeability and reinforcing tight junctions. Probiotic Escherichia coli Nissle 1917 (EcN) is a good colonizer of the human gut with proven therapeutic efficacy in the remission of ulcerative colitis in humans...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27934011/a-mechanistic-model-for-colibactin-induced-genotoxicity
#9
Alan R Healy, Herman Nikolayevskiy, Jaymin R Patel, Jason M Crawford, Seth B Herzon
Precolibactins and colibactins represent a family of natural products that are encoded by the clb gene cluster and are produced by certain commensal, extraintestinal, and probiotic E. coli. clb(+) E. coli induce megalocytosis and DNA double-strand breaks in eukaryotic cells, but paradoxically, this gene cluster is found in the probiotic Nissle 1917. Evidence suggests precolibactins are converted to genotoxic colibactins by colibactin peptidase (ClbP)-mediated cleavage of an N-acyl-d-Asn side chain, and all isolation efforts have employed ΔclbP strains to facilitate accumulation of precolibactins...
December 7, 2016: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/27878261/bacterial-ghosts-as-adjuvants-in-syngeneic-tumour-cell-lysate-based-anticancer-vaccination-in-a-murine-lung-carcinoma-model
#10
Jan Aleksander Kraśko, Karolina Žilionytė, Adas Darinskas, Marius Strioga, Svetlana Rjabceva, Iosif Zalutsky, Marina Derevyanko, Vladimir Kulchitsky, Werner Lubitz, Pavol Kudela, Edita Miseikyte-Kaubriene, Olha Karaman, Hennadii Didenko, Hryhorii Potebnya, Vasyl Chekhun, Vita Pašukonienė
Instead of relying on external anticancer factors for treatment, immunotherapy utilizes the host's own immune system and directs it against given tumour antigens. This study demonstrated that it is possible to overcome the documented immunosuppressive properties of tumour cell lysate by supplementing it with appropriate adjuvant. Lewis lung carcinoma (LLC)‑challenged C57BL/6 mice were treated with LLC cryo‑lysate mixed with either bacterial ghosts (BGs) generated from E. coli Nissle 1917 or B. subtilis 70 kDa protein as adjuvants...
January 2017: Oncology Reports
https://www.readbyqxmd.com/read/27798599/microcins-mediate-competition-among-enterobacteriaceae-in-the-inflamed-gut
#11
Martina Sassone-Corsi, Sean-Paul Nuccio, Henry Liu, Dulcemaria Hernandez, Christine T Vu, Amy A Takahashi, Robert A Edwards, Manuela Raffatellu
The Enterobacteriaceae are a family of Gram-negative bacteria that include commensal organisms as well as primary and opportunistic pathogens that are among the leading causes of morbidity and mortality worldwide. Although Enterobacteriaceae often comprise less than 1% of a healthy intestine's microbiota, some of these organisms can bloom in the inflamed gut; expansion of enterobacteria is a hallmark of microbial imbalance known as dysbiosis. Microcins are small secreted proteins that possess antimicrobial activity in vitro, but whose role in vivo has been unclear...
December 8, 2016: Nature
https://www.readbyqxmd.com/read/27795372/escherichia-coli-probiotic-strain-ed1a-in-pigs-has-a-limited-impact-on-the-gut-carriage-of-extended-spectrum-%C3%AE-lactamase-producing-e-coli
#12
G Mourand, F Paboeuf, M A Fleury, E Jouy, S Bougeard, E Denamur, I Kempf
Four trials were conducted to evaluate the impact of Escherichia coli probiotic strain ED1a administration to pigs on the gut carriage or survival in manure of extended-spectrum-β-lactamase-producing E. coli Groups of pigs were orally inoculated with strain E. coli M63 carrying the blaCTX-M-1 gene (n = 84) or used as a control (n = 26). In the first two trials, 24 of 40 E. coli M63-inoculated pigs were given E. coli ED1a orally for 6 days starting 8 days after oral inoculation. In the third trial, 10 E. coli M63-inoculated pigs were given either E...
January 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27782051/pmpes-a-modular-peptide-expression-system-for-the-delivery-of-antimicrobial-peptides-to-the-site-of-gastrointestinal-infections-using-probiotics
#13
Kathryn Geldart, Brittany Forkus, Evelyn McChesney, Madeline McCue, Yiannis N Kaznessis
Antimicrobial peptides are a promising alternative to traditional antibiotics, but their utility is limited by high production costs and poor bioavailability profiles. Bacterial production and delivery of antimicrobial peptides (AMPs) directly at the site of infection may offer a path for effective therapeutic application. In this study, we have developed a vector that can be used for the production and secretion of seven antimicrobial peptides from both Escherichia coli MC1061 F' and probiotic E.coli Nissle 1917...
October 5, 2016: Pharmaceuticals
https://www.readbyqxmd.com/read/27766164/insights-from-100-years-of-research-with-probiotic-e-coli
#14
REVIEW
Trudy M Wassenaar
A century ago, Alfred Nissle discovered that intentional intake of particular strains of Escherichia coli could treat patients suffering from infectious diseases. Since then, one of these strains became the most frequently used probiotic E. coli in research and was applied to a variety of human conditions. Here, properties of that E. coli Nissle 1917 strain are compared with other commercially available E. coli probiotic strains, with emphasis on their human applications. A literature search formed the basis of a summary of research findings reported for the probiotics Mutaflor, Symbioflor 2, and Colinfant...
September 29, 2016: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/27686530/extraintestinal-pathogenic-escherichia-coli-are-associated-with-intestinal-inflammation-in-patients-with-ulcerative-colitis
#15
Hengameh C Mirsepasi-Lauridsen, Sofie Ingdam Halkjaer, Esben Munk Mortensen, Magnus C Lydolph, Inge Nordgaard-Lassen, Karen Angeliki Krogfelt, Andreas Munk Petersen
E. coli of the phylogenetic group B2 harbouring Extra intestinal Pathogenic Escherichia coli (ExPEC) genes are frequently seen as colonizers of the intestine in patients with active ulcerative colitis (UC). In this study, we describe the influence of E. coli Nissle (EcN) B2 as add-on treatment to conventional therapies in patients with active UC. For this study one hundred active UC patients were randomized to ciprofloxacin or placebo for 1 week followed by EcN or placebo for 7 weeks. Stool samples were collected at weeks 0, 1, 8, 12, where E...
September 30, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27619890/escherichia-coli-strain-nissle-1917-from-bench-to-bedside-and-back-history-of-a-special-escherichia-coli-strain-with-probiotic-properties
#16
Ulrich Sonnenborn
Among the gram-negative microorganisms with probiotic properties, Escherichia coli strain Nissle 1917 (briefly EcN) is probably the most intensively investigated bacterial strain today. Since nearly 100 years, the EcN strain is used as the active pharmaceutical ingredient in a licensed medicinal product that is distributed in Germany and several other countries. Over the last few decades, novel probiotic activities have been detected, which taken together are specific of this versatile E. coli strain. This review gives a short overview on the discovery and history of the EcN strain...
October 2016: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/27542382/synthesis-of-natural-variants-and-synthetic-derivatives-of-the-cyclic-nonribosomal-peptide-luminmide-in-permeabilized-e-coli-nissle-and-product-formation-kinetics
#17
Christian Weyler, Elmar Heinzle
We used a recombinant, permeabilized E. coli Nissle strain harbouring the plu3263 gene cluster from Photorhabdus luminescens for the synthesis of luminmide type cyclic pentapeptides belonging to the class of nonribosomally biosynthesized peptides (NRP). Cells could be fully permeabilized using 1 % v/v toluene. Synthesis of luminmides was increased fivefold when 0.3 mM EDTA was added to the substrate mixture acting as an inhibitor of metal proteases. Luminmide formation was studied applying different amino acid concentrations...
January 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27487076/outer-membrane-vesicles-from-the-probiotic-escherichia-coli-nissle-1917-and-the-commensal-ecor12-enter-intestinal-epithelial-cells-via-clathrin-dependent-endocytosis-and-elicit-differential-effects-on-dna-damage
#18
María-Alexandra Cañas, Rosa Giménez, María-José Fábrega, Lorena Toloza, Laura Baldomà, Josefa Badia
Interactions between intestinal microbiota and the human host are complex. The gut mucosal surface is covered by a mucin layer that prevents bacteria from accessing the epithelial cells. Thus, the crosstalk between microbiota and the host mainly rely on secreted factors that can go through the mucus layer and reach the epithelium. In this context, vesicles released by commensal strains are seen as key players in signaling processes in the intestinal mucosa. Studies with Gram-negative pathogens showed that outer membrane vesicles (OMVs) are internalized into the host cell by endocytosis, but the entry mechanism for microbiota-derived vesicles is unknown...
2016: PloS One
https://www.readbyqxmd.com/read/27372848/use-of-escherichia-coli-nissle-1917-producing-recombinant-colicins-for-treatment-of-ibd-patients
#19
Roman Kotłowski
Patients with Crohn's Disease and Ulcerative Colitis infected with Adherent-Invasive Escherichia coli strains constitute the largest group among Inflammatory Bowel Disease subjects, when taking into account all known etiological agents of the disease. A possible link between these pathogenic bacteria and inflammation process has gained the confidence in recently published papers. Observed enteric neuroglial cells apoptosis and epithelial gaps of ileum are probably the key manifestations of inflammation, which has been shown in IBD patients in contrary to the samples taken from healthy individuals...
August 2016: Medical Hypotheses
https://www.readbyqxmd.com/read/27340358/intestinal-borne-dermatoses-significantly-improved-by-oral-application-of-escherichia-coli-nissle-1917
#20
Elina Manzhalii, Daniel Hornuss, Wolfgang Stremmel
AIM: To evaluate the effect of oral Escherichia coli (E. coli) Nissle application on the outcome of intestinal-borne dermatoses. METHODS: In a randomized, controlled, non-blinded prospective clinical trial 82 patients with intestinal-borne facial dermatoses characterized by an erythematous papular-pustular rash were screened. At the initiation visit 37 patients entered the experimental arm and 20 patients constituted the control arm. All 57 patients were treated with a vegetarian diet and conventional topical therapy of the dermatoses with ointments containing tetracycline, steroids and retinoids...
June 21, 2016: World Journal of Gastroenterology: WJG
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