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E. coli Nissle

Jan Aleksander Kraśko, Karolina Žilionytė, Adas Darinskas, Marius Strioga, Svetlana Rjabceva, Iosif Zalutsky, Marina Derevyanko, Vladimir Kulchitsky, Werner Lubitz, Pavol Kudela, Edita Miseikyte-Kaubriene, Olha Karaman, Hennadii Didenko, Hryhorii Potebnya, Vasyl Chekhun, Vita Pašukonienė
Instead of relying on external anticancer factors for treatment, immunotherapy utilizes the host's own immune system and directs it against given tumour antigens. This study demonstrated that it is possible to overcome the documented immunosuppressive properties of tumour cell lysate by supplementing it with appropriate adjuvant. Lewis lung carcinoma (LLC)‑challenged C57BL/6 mice were treated with LLC cryo‑lysate mixed with either bacterial ghosts (BGs) generated from E. coli Nissle 1917 or B. subtilis 70 kDa protein as adjuvants...
November 16, 2016: Oncology Reports
Martina Sassone-Corsi, Sean-Paul Nuccio, Henry Liu, Dulcemaria Hernandez, Christine T Vu, Amy A Takahashi, Robert A Edwards, Manuela Raffatellu
The Enterobacteriaceae are Gram-negative bacteria and include commensal organisms as well as primary and opportunistic pathogens that are among the leading causes of morbidity and mortality worldwide. Although Enterobacteriaceae often comprise less than 1% of a healthy intestine's microbiota(1), some of these organisms can bloom in the inflamed gut(2,3,4,5); indeed, expansion of enterobacteria is a hallmark of microbial imbalance known as "dysbiosis"(6). Microcins are small secreted proteins that possess antimicrobial activity in vitro(7,8), but whose role in vivo has been unclear...
October 31, 2016: Nature
G Mourand, F Paboeuf, M A Fleury, E Jouy, S Bougeard, E Denamur, I Kempf
Four trials were conducted to evaluate the impact of Escherichia coli probiotic strain ED1a (EcED1a) administration to pigs on the gut carriage or survival in manure of extended-spectrum beta-lactamase-producing E. coli Groups of pigs were orally inoculated with strain E. coli M63 (EcM63) carrying the blaCTX-M-1 gene (n=84) or used as control (n=26). In the first two trials, eight days later, 24 of 40 EcM63 inoculated pigs were given EcED1a orally for six days. In the third trial, 10 EcM63 inoculated pigs were given either EcED1a or probiotic E...
October 17, 2016: Antimicrobial Agents and Chemotherapy
Kathryn Geldart, Brittany Forkus, Evelyn McChesney, Madeline McCue, Yiannis N Kaznessis
Antimicrobial peptides are a promising alternative to traditional antibiotics, but their utility is limited by high production costs and poor bioavailability profiles. Bacterial production and delivery of antimicrobial peptides (AMPs) directly at the site of infection may offer a path for effective therapeutic application. In this study, we have developed a vector that can be used for the production and secretion of seven antimicrobial peptides from both Escherichia coli MC1061 F' and probiotic E.coli Nissle 1917...
October 5, 2016: Pharmaceuticals
Trudy M Wassenaar
A century ago, Alfred Nissle discovered that intentional intake of particular strains of Escherichia coli could treat patients suffering from infectious diseases. Since then, one of these strains became the most frequently used probiotic E. coli in research and was applied to a variety of human conditions. Here, properties of that E. coli Nissle 1917 strain are compared with other commercially available E. coli probiotic strains, with emphasis on their human applications. A literature search formed the basis of a summary of research findings reported for the probiotics Mutaflor, Symbioflor 2, and Colinfant...
September 29, 2016: European Journal of Microbiology & Immunology
Hengameh C Mirsepasi-Lauridsen, Sofie Ingdam Halkjaer, Esben Munk Mortensen, Magnus C Lydolph, Inge Nordgaard-Lassen, Karen Angeliki Krogfelt, Andreas Munk Petersen
E. coli of the phylogenetic group B2 harbouring Extra intestinal Pathogenic Escherichia coli (ExPEC) genes are frequently seen as colonizers of the intestine in patients with active ulcerative colitis (UC). In this study, we describe the influence of E. coli Nissle (EcN) B2 as add-on treatment to conventional therapies in patients with active UC. For this study one hundred active UC patients were randomized to ciprofloxacin or placebo for 1 week followed by EcN or placebo for 7 weeks. Stool samples were collected at weeks 0, 1, 8, 12, where E...
September 30, 2016: Scientific Reports
Ulrich Sonnenborn
Among the gram-negative microorganisms with probiotic properties, Escherichia coli strain Nissle 1917 (briefly EcN) is probably the most intensively investigated bacterial strain today. Since nearly 100 years, the EcN strain is used as the active pharmaceutical ingredient in a licensed medicinal product that is distributed in Germany and several other countries. Over the last few decades, novel probiotic activities have been detected, which taken together are specific of this versatile E. coli strain. This review gives a short overview on the discovery and history of the EcN strain...
October 2016: FEMS Microbiology Letters
Christian Weyler, Elmar Heinzle
We used a recombinant, permeabilized E. coli Nissle strain harbouring the plu3263 gene cluster from Photorhabdus luminescens for the synthesis of luminmide type cyclic pentapeptides belonging to the class of nonribosomally biosynthesized peptides (NRP). Cells could be fully permeabilized using 1 % v/v toluene. Synthesis of luminmides was increased fivefold when 0.3 mM EDTA was added to the substrate mixture acting as an inhibitor of metal proteases. Luminmide formation was studied applying different amino acid concentrations...
August 20, 2016: Applied Microbiology and Biotechnology
María-Alexandra Cañas, Rosa Giménez, María-José Fábrega, Lorena Toloza, Laura Baldomà, Josefa Badia
Interactions between intestinal microbiota and the human host are complex. The gut mucosal surface is covered by a mucin layer that prevents bacteria from accessing the epithelial cells. Thus, the crosstalk between microbiota and the host mainly rely on secreted factors that can go through the mucus layer and reach the epithelium. In this context, vesicles released by commensal strains are seen as key players in signaling processes in the intestinal mucosa. Studies with Gram-negative pathogens showed that outer membrane vesicles (OMVs) are internalized into the host cell by endocytosis, but the entry mechanism for microbiota-derived vesicles is unknown...
2016: PloS One
Roman Kotłowski
Patients with Crohn's Disease and Ulcerative Colitis infected with Adherent-Invasive Escherichia coli strains constitute the largest group among Inflammatory Bowel Disease subjects, when taking into account all known etiological agents of the disease. A possible link between these pathogenic bacteria and inflammation process has gained the confidence in recently published papers. Observed enteric neuroglial cells apoptosis and epithelial gaps of ileum are probably the key manifestations of inflammation, which has been shown in IBD patients in contrary to the samples taken from healthy individuals...
August 2016: Medical Hypotheses
Elina Manzhalii, Daniel Hornuss, Wolfgang Stremmel
AIM: To evaluate the effect of oral Escherichia coli (E. coli) Nissle application on the outcome of intestinal-borne dermatoses. METHODS: In a randomized, controlled, non-blinded prospective clinical trial 82 patients with intestinal-borne facial dermatoses characterized by an erythematous papular-pustular rash were screened. At the initiation visit 37 patients entered the experimental arm and 20 patients constituted the control arm. All 57 patients were treated with a vegetarian diet and conventional topical therapy of the dermatoses with ointments containing tetracycline, steroids and retinoids...
June 21, 2016: World Journal of Gastroenterology: WJG
Harshana Sabharwal, Christoph Cichon, Tobias A Ölschläger, Ulrich Sonnenborn, M Alexander Schmidt
Bacterium-host interactions in the gut proceed via directly contacted epithelial cells, the host's immune system, and a plethora of bacterial factors. Here we characterized and compared exemplary cytokine and microRNA (miRNA) responses of human epithelial and THP-1 cells toward the prototype enteropathogenic Escherichia coli (EPEC) strain E2348/69 (O127:H6) and the probiotic strain Escherichia coli Nissle 1917 (EcN) (O6:K5:H1). Human T84 and THP-1 cells were used as cell culture-based model systems for epithelial and monocytic cells...
September 2016: Infection and Immunity
María José Fábrega, Laura Aguilera, Rosa Giménez, Encarna Varela, María Alexandra Cañas, María Antolín, Josefa Badía, Laura Baldomà
The influence of microbiota in human health is well-known. Imbalances in microbiome structure have been linked to several diseases. Modulation of microbiota composition through probiotic therapy is an attempt to harness the beneficial effects of commensal microbiota. Although, there is wide knowledge of the responses induced by gut microbiota, the microbial factors that mediate these effects are not well-known. Gram-negative bacteria release outer membrane vesicles (OMVs) as a secretion mechanism of microbial factors, which have an important role in intercellular communication...
2016: Frontiers in Microbiology
Anice Sabag-Daigle, Henry M Blunk, Juan F Gonzalez, Brandi L Steidley, Prosper N Boyaka, Brian M M Ahmer
Salmonella enterica is among the most burdensome of foodborne disease agents. There are over 2,600 serovars that cause a range of disease manifestations ranging from enterocolitis to typhoid fever. While there are two vaccines in use in humans to protect against typhoid fever, there are none that prevent enterocolitis. If vaccines preventing enterocolitis were to be developed, they would likely protect against only one or a few serovars. In this report, we tested the hypothesis that probiotic organisms could compete for the preferred nutrient sources of Salmonella and thus prevent or treat infection...
July 2016: Infection and Immunity
Lauranne A A P Derikx, Levinus A Dieleman, Frank Hoentjen
The intestinal microbiota is one of the key players in the etiology of ulcerative colitis. Manipulation of this microflora with probiotics and prebiotics is an attractive strategy in the management of ulcerative colitis. Several intervention studies for both the induction and maintenance of remission in ulcerative colitis patients have been performed. Most of these studies evaluated VSL#3 or E. Coli Nissle 1917 and in general there is evidence for efficacy of these agents for induction and maintenance of remission...
February 2016: Best Practice & Research. Clinical Gastroenterology
Hengameh Chloé Mirsepasi-Lauridsen, Zhengyu Du, Carsten Struve, Godefroid Charbon, Jurgen Karczewski, Karen Angeliki Krogfelt, Andreas Munk Petersen, Jerry M Wells
OBJECTIVES: The potential of Escherichia coli (E. coli) isolated from inflammatory bowel disease (IBD) patients to damage the integrity of the intestinal epithelium was investigated. METHODS: E. coli strains isolated from patients with ulcerative colitis (UC) and healthy controls were tested for virulence capacity by molecular techniques and cytotoxic assays and transepithelial electric resistance (TER). E. coli isolate p19A was selected, and deletion mutants were created for alpha-hemolysin (α-hemolysin) (hly) clusters and cytotoxic necrotizing factor type 1 (cnf1)...
2016: Clinical and Translational Gastroenterology
Panos Lehouritis, Michael Stanton, Florence O McCarthy, Matthieu Jeavons, Mark Tangney
Some chemotherapeutic drugs (prodrugs) require activation by an enzyme for efficacy. We and others have demonstrated the ability of probiotic bacteria to grow specifically within solid tumours following systemic administration, and we hypothesised that the natural enzymatic activity of these tumour-localised bacteria may be suitable for activation of certain such chemotherapeutic drugs. Several wild-type probiotic bacteria; Escherichia coli Nissle, Bifidobacterium breve, Lactococcus lactis and Lactobacillus species, were screened against a panel of popular prodrugs...
January 28, 2016: Journal of Controlled Release: Official Journal of the Controlled Release Society
Amir H Faghihi, Shahram Agah, Mohsen Masoudi, Seyed M S Ghafoori, Abbas Eshraghi
AIM: to evaluate potential improvement effect for probiotic E. coliNissle 1917 in the management of refractory IBS in an Iranian population. METHODS: a double blind placebo controlled approach has been used in the current clinical trial. 139 confirmed IBS patients were included into the study, and were given probiotic E.coli Nissle 1917 for 6 weeks. 11 items Birmingham IBS Symptom Questionnairehas been used for evaluation of changes in the symptoms every 2 weeks...
July 2015: Acta Medica Indonesiana
Stina Rikke Jensen, Hengameh Chloé Mirsepasi-Lauridsen, Anna Hammerich Thysen, Jørn Brynskov, Karen A Krogfelt, Andreas Munk Petersen, Anders Elm Pedersen, Susanne Brix
Escherichia coli (E. coli) may be implicated in the pathogenesis of inflammatory bowel disease (IBD), as implied from a higher prevalence of mucosa-associated E. coli in the gut of IBD-affected individuals. However, it is unclear whether different non-diarrheagenic E. coli spp. segregate from each other in their ability to promote intestinal inflammation. Herein we compared the inflammation-inducing properties of non-diarrheagenic LF82, 691-04A, E. coli Nissle 1917 (ECN) and eleven new intestinal isolates from different locations in five IBD patients and one healthy control...
December 2015: International Journal of Medical Microbiology: IJMM
Timo Langemann, Ulrike Beate Mayr, Andrea Meitz, Werner Lubitz, Christoph Herwig
Flow cytometry (FCM) is a tool for the analysis of single-cell properties in a cell suspension. In this contribution, we present an improved FCM method for the assessment of E-lysis in Enterobacteriaceae. The result of the E-lysis process is empty bacterial envelopes-called bacterial ghosts (BGs)-that constitute potential products in the pharmaceutical field. BGs have reduced light scattering properties when compared with intact cells. In combination with viability information obtained from staining samples with the membrane potential-sensitive fluorescent dye bis-(1,3-dibutylarbituric acid) trimethine oxonol (DiBAC4(3)), the presented method allows to differentiate between populations of viable cells, dead cells, and BGs...
January 2016: Applied Microbiology and Biotechnology
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