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https://www.readbyqxmd.com/read/28088611/lactobacillus-paracasei-modulates-lps-induced-inflammatory-cytokine-release-by-monocyte-macrophages-via-the-up-regulation-of-negative-regulators-of-nf-kappab-signaling-in-a-tlr2-dependent-manner
#1
Ke-Yi Sun, Dong-Hua Xu, Chao Xie, Susan Plummer, James Tang, Xiao Fan Yang, Xiao Hui Ji
The application of the probiotic lactobacillus is suggested in the treatment of some inflammatory diseases of intestines due to its potential ability to attenuate inflammation. However, the mechanism is not completely understood. In PBMCs, Lactobacillus paracasei (L. Paracasei) down-regulated the LPS-induced production of TNF-α and IL-6. Using a macrophage-like differentiated THP-1 cell line induced by PMA, we investigated the effect of L. paracasei on the production of pro-inflammatory cytokines by monocyte-macrophages...
January 12, 2017: Cytokine
https://www.readbyqxmd.com/read/28088418/laboratory-silo-type-and-inoculation-effects-on-nutritional-composition-fermentation-and-bacterial-and-fungal-communities-of-oat-silage
#2
J J Romero, Y Zhao, M A Balseca-Paredes, F Tiezzi, E Gutierrez-Rodriguez, M S Castillo
The objectives were to evaluate (1) the use of 2 types of experimental silos (S) to characterize whole-crop oat (Avena sativa L.) silage with or without addition of an inoculant (I), and (2) the effect of inoculation on the microbial community structure of oats ensiled using only plastic bucket silos (BKT). From each of 6 sections in a field, oats were harvested, treated (INO) or not (CON) with inoculant, packed into 19-L BKT or vacuum bags (BG), and ensiled for 217 d. The inoculant added contained Lactobacillus buchneri and Pediococcus pentosaceus (4 × 10(5) and 1 × 10(5) cfu/g of fresh oats, respectively)...
January 11, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28087949/the-vaginal-microbiota-and-urinary-tract-infection
#3
Ann E Stapleton
The vagina is a key anatomical site in the pathogenesis of urinary tract infection (UTI) in women, serving as a potential reservoir for infecting bacteria and a site at which interventions may decrease the risk of UTI. The vaginal microbiota is a dynamic and often critical factor in this pathogenic interplay, because changes in the characteristics of the vaginal microbiota resulting in the loss of normally protective Lactobacillus spp. increase the risk of UTI. These alterations may result from the influence of estrogen deficiency, antimicrobial therapy, contraceptives, or other causes...
December 2016: Microbiology Spectrum
https://www.readbyqxmd.com/read/28087614/usefulness-of-ftir-spectroscopy-to-distinguish-rough-and-smooth-variants-of-lactobacillus-farciminis-cncm-i-3699
#4
R Tareb, M Bernardeau, C Amiel, J P Vernoux
In this study, the potential of FTIR spectroscopy for assessing putative biochemical and structural differences between the two variants Rough (R) and Smooth (S) of Lactobacillus farciminis CNCM-I-3699, a pleomorphic strain, was investigated. The main differences observed were localized in the polysaccharide (1200-900 cm(-1)) and protein (1700-1500 cm(-1)) regions. Based on spectral information in these two spectral ranges, clustering resulted in a dendrogram that showed a clear discrimination between both morphotypes...
January 12, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28087537/effects-of-lactobacillus-plantarum-oll2712-culture-conditions-on-the-anti-inflammatory-activity-for-murine-immune-cells-and-obese-and-type-2-diabetic-mice
#5
T Toshimitsu, S Ozaki, J Mochizuki, K Furuichi, Y Asami
: Studies on the health-promoting effects of lactic acid bacteria (LAB) are numerous, but few provide examples of the relationship between LAB function and culture conditions. We verified the effect of differences in culture conditions on Lactobacillus plantarum OLL2712 (OLL2712) functionality; this strain exhibits anti-inflammatory activity and preventive effects against metabolic disorders. We measured IL-10 and IL-12 production in murine immune cells treated with OLL2712 cells prepared under various culture conditions...
January 13, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28087493/antioxidant-and-antiproliferative-potential-of-biosurfactants-isolated-from-lactobacillus-casei-and-their-anti-biofilm-effect-in-oral-staphylococcus-aureus-strains
#6
Abderrahmen Merghni, Ines Dallel, Emira Noumi, Yassine Kadmi, Hajer Hentati, Samir Tobji, Adel Ben Amor, Maha Mastouri
Biosurfactants also called bioemulsifiers are amphipathic compounds produced by many microorganisms that allow them to exhibit a wide range of biological activities. The aim of this study was to determine the antioxidant and antiproliferative potential of biosurfactants isolated from Lactobacillus casei and to assess their anti-adhesive and anti-biofilm abilities against oral opportunistic Staphylococcus aureus strains. The antioxidant activity of biosurfactant was evaluated using the in vitro scavenging ability on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical...
January 10, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28087240/lactobacillus-deficient-cervicovaginal-bacterial-communities-are-associated-with-increased-hiv-acquisition-in-young-south-african-women
#7
Christina Gosmann, Melis N Anahtar, Scott A Handley, Mara Farcasanu, Galeb Abu-Ali, Brittany A Bowman, Nikita Padavattan, Chandni Desai, Lindsay Droit, Amber Moodley, Mary Dong, Yuezhou Chen, Nasreen Ismail, Thumbi Ndung'u, Musie S Ghebremichael, Duane R Wesemann, Caroline Mitchell, Krista L Dong, Curtis Huttenhower, Bruce D Walker, Herbert W Virgin, Douglas S Kwon
Elevated inflammation in the female genital tract is associated with increased HIV risk. Cervicovaginal bacteria modulate genital inflammation; however, their role in HIV susceptibility has not been elucidated. In a prospective cohort of young, healthy South African women, we found that individuals with diverse genital bacterial communities dominated by anaerobes other than Gardnerella were at over 4-fold higher risk of acquiring HIV and had increased numbers of activated mucosal CD4(+) T cells compared to those with Lactobacillus crispatus-dominant communities...
January 9, 2017: Immunity
https://www.readbyqxmd.com/read/28081358/microbial-diversity-during-fermentation-of-sweet-paste-a-chinese-traditional-seasoning-using-pcr-denaturing-gradient-gel-electrophoresis
#8
Ping Mao, Yuanliang Hu, Tingting Liao, Zhaoting Wang, Shumiao Zhao, Yunxiang Liang, Yongmei Hu
The aim of this study was to elucidate the changes in the microbial community and biochemical properties of a traditional sweet paste during fermentation. PCR-denaturing gradient gel electrophoresis (DGGE) analysis showed that Aspergillus oryzae was the predominant species in the koji and the majority of the fungi isolated belonged to 2 Zygosaccharomyces species in the mash. The bacterial DGGE profiles revealed the presence of Bacillus subtilis during fermentation, and Lactobacillus acidipiscis, Lactobacillus pubuzihii, Lactobacillus sp, Staphylococcus kloosi and several uncultured bacteria were also detected in the mash after 14 days of main fermentation...
January 12, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28081167/bacterial-communities-associated-with-houseflies-musca-domestica-l-sampled-within-and-between-farms
#9
Simon Bahrndorff, Nadieh de Jonge, Henrik Skovgård, Jeppe Lund Nielsen
The housefly feeds and reproduces in animal manure and decaying organic substances and thus lives in intimate association with various microorganisms including human pathogens. In order to understand the variation and association between bacteria and the housefly, we used 16S rRNA gene amplicon sequencing to describe bacterial communities of 90 individual houseflies collected within and between ten dairy farms in Denmark. Analysis of gene sequences showed that the most abundant classes of bacteria found across all sites included Bacilli, Clostridia, Actinobacteria, Flavobacteria, and all classes of Proteobacteria and at the genus level the most abundant genera included Corynebacterium, Lactobacillus, Staphylococcus, Vagococcus, Weissella, Lactococcus, and Aerococcus...
2017: PloS One
https://www.readbyqxmd.com/read/28079729/effect-of-probiotics-on-diarrhea-in-children-with-severe-acute-malnutrition-a-randomized-controlled-study-in-uganda
#10
Benedikte Grenov, Hanifa Namusoke, Betty Lanyero, Nicolette Nabukeera-Barungi, Christian Ritz, Christian Mølgaard, Henrik Friis, Kim F Michaelsen
OBJECTIVES: To assess the effect of probiotics on diarrhea during in- and outpatient treatment of children with severe acute malnutrition (SAM). METHODS: A randomized, double-blind, placebo-controlled study was conducted involving 400 children admitted with SAM. Patients received one daily dose of a blend of Bifidobacterium animalis subsp. lactis (BB-12) and Lactobacillus rhamnosus (LGG) (10 billion colony-forming units, 50:50) or placebo during hospitalization followed by an 8-12 week outpatient treatment period, depending on patients' recovery rate...
January 11, 2017: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/28077192/dietary-probiotic-supplementation-improves-growth-and-the-intestinal-morphology-of-nile-tilapia
#11
M A Ramos, S Batista, M A Pires, A P Silva, L F Pereira, M J Saavedra, R O A Ozório, P Rema
Probiotic administration can be a nutritional strategy to improve the immune response and growth performance of fish. The current study aimed to evaluate the effects of a probiotic blend (Bacillus sp., Pediococcus sp., Enterococcus sp., Lactobacillus sp.) as a dietary supplement on growth performance, feed utilization, innate immune and oxidative stress responses and intestinal morphology in juvenile Nile tilapia (Oreochromis niloticus). The probiotic was incorporated into a basal diet at three concentrations: 0 g/kg (A0: control), 3 g/kg (A1: 1...
January 12, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28076655/fecal-total-iron-concentration-is-inversely-associated-with-fecal-lactobacillus-in-preschool-children
#12
Sasank Kalipatnapu, Sivaraman Kuppuswamy, Giriprasad Venugopal, Venkatesh Kaliaperumal, Balamurugan Ramadass
BACKGROUND: Iron deficiency is associated with stunting and poor performance in children. Oral iron supplementation is widely promoted to correct iron deficiency. However, excess iron may be toxic to beneficial luminal gut bacteria and could support growth of pathobionts. OBJECTIVE: To analyze the fecal total iron concentration and fecal Lactobacillus levels in a cohort of stunted and normal children. DESIGN: The study was undertaken in two different locations...
January 11, 2017: Journal of Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28075415/inhibitory-effects-of-probiotic-lactobacillus-on-the-growth-of-human-colonic-carcinoma-cell-line-ht-29
#13
Zhung-Yuan Chen, You-Miin Hsieh, Chun-Chih Huang, Cheng-Chih Tsai
This study was conducted to investigate the inhibitory effect of Lactobacillus cells and supernatants on the growth of the human colon cancer cell line HT-29. Our study results indicated that the PM153 strain exhibits the best adhesion ability and the highest survival in the gastrointestinal tract simulation experiment. Furthermore, after an 8-h co-culture of PM153 and HT-29 cells, the PM153 strain can induce the secretion of nitric oxide from the HT-29 cells. In addition, after the co-culture of the BCRC17010 strain (10⁸ cfu/mL) and HT-29 cells, the Bax/Bcl-2 ratio in the HT-29 cells was 1...
January 10, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28074388/lactobacillus-slpa-promotes-esc-growth-through-the-erk1-2-pathway
#14
Jinying He, Ya Tuo, Wenjie Yan, Jing Yang
Bacterial surface layers (S-layers) are cell envelope structures ubiquitously found in gram-negative and gram-positive bacteria, including Lactobacillus. S-layers play a role in the determination and maintenance of cell shape as virulence factors, mediate cell adhesion, and regulate immature dendritic and T cells. In this study, we sought to understand the involvement of MAPK serine/threonine kinases in alterations in Endometrial epithelial cells (ESC) growth induced by Lactobacillus crispatus (L. crispatus) slpA, an S-layer protein...
January 10, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28073366/oral-treatment-with-lactobacillus-rhamnosus-attenuates-behavioural-deficits-and-immune-changes-in-chronic-social-stress
#15
Aadil Bharwani, M Firoz Mian, Michael G Surette, John Bienenstock, Paul Forsythe
BACKGROUND: Stress-related disorders involve systemic alterations, including disruption of the intestinal microbial community. Given the putative connections between the microbiota, immunity, neural function, and behaviour, we investigated the potential for microbe-induced gut-to-brain signalling to modulate the impact of stress on host behaviour and immunoregulation. METHODS: Male C57BL/6 mice treated orally over 28 days with either Lactobacillus rhamnosus (JB-1) ™ or vehicle were subjected to chronic social defeat and assessed for alterations in behaviour and immune cell phenotype...
January 11, 2017: BMC Medicine
https://www.readbyqxmd.com/read/28071802/essential-oils-against-pathogen-and-spoilage-microorganisms-of-fruit-juices-use-of-versatile-antimicrobial-delivery-systems
#16
Valentina Trinetta, Mark T Morgan, John N Coupland, Umut Yucel
Essential oils (EO) are increasingly used as natural antimicrobial compounds, however the effect of delivery system to enhance their antimicrobial activity has not been widely studied. Limonene (0 to 10 μL/mL) was added to microbial suspensions (∼10(5) CFU/mL) of selected foodborne pathogens (Listeria monocytogenes Scott A, Salmonella enterica Typhimurium, Escherichia coli and Staphylococcus aureus), and spoilage microorganisms (Lactobacillus plantarum, Saccharomyces cerevisiae, and Candida albicans). S...
January 10, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28071017/evaluation-of-the-potential-use-of-probiotic-strain-lactobacillus-plantarum-299v-in-lactic-fermentation-of-button-mushroom-fruiting-bodies
#17
Ewa Jabłońska-Ryś, Aneta Sławińska, Wojciech Radzki, Waldemar Gustaw
BACKGROUND: The available literature does not provide data on the application of probiotic strains in mushroom processing. The aim of the study was to evaluate the potential to use the L. plantarum 299v strain with documented probiotic properties in the process of lactic fermentation of button mushroom fruiting bodies (Agaricus bisporus). METHODS: Fresh button mushroom fruiting bodies and cultures of lactic acid bacteria L. plantarum Ib and a probiotic strain L...
October 2016: Acta Scientiarum Polonorum. Technologia Alimentaria
https://www.readbyqxmd.com/read/28071015/the-changes-of-proteins-fractions-shares-in-milk-and-fermented-milk-drinks
#18
Genowefa Bonczar, Maria Walczycka, Iwona Duda
BACKGROUND: The aim of this research was to observe the changes which take place in the electrophoretic picture of milk proteins after pasteurisation and inoculation with different starter cultures (both traditional and probiotic). After incubation, the yoghurt, kefir, acidified milk, fermented Bifidobacterium bifidum drink and Lactobacillus acidophillus drink were chilled for 14 days to observe the changes which occurred. METHODS: The research materials were raw and pasteurised milk, as well as fermented milk- based drinks...
October 2016: Acta Scientiarum Polonorum. Technologia Alimentaria
https://www.readbyqxmd.com/read/28071014/properties-of-ice-cream-fortified-with-zinc-and-lactobacillus-casei
#19
Hamid R Gheisari, Leila Ahadi, Sanaz Khezli, Tayebeh Dehnavi
BACKGROUND: In this study, the possible effects of zinc on physicochemical properties of ice cream and the survival of Lactobacillus casei during a 90 days storage at -18°C was investigated. METHODS: Samples were divided into four experimental groups as follows: control, zinc fortified ice cream, probiotic ice cream, zinc fortified and probiotic ice cream. The physicochemical, texture, organoleptic properties and the survival of probiotics, were investigated. RESULTS: Results showed that the addition of zinc did not affect the textural properties of ice creams...
October 2016: Acta Scientiarum Polonorum. Technologia Alimentaria
https://www.readbyqxmd.com/read/28071005/fermentation-optimization-of-goat-milk-with-lactobacillus-acidophilus-and-bifidobacterium-bifidum-by-box-behnken-design
#20
Guowei Shu, Chunju Bao, He Chen, Changfeng Wang, Hui Yang
BACKGROUND: Goat milk is only limited to the processing of goat milk powder and liquid milk, the products are mainly about milk powder and a few of them are made as milk tablet. Therefore, the study of probiotic goat milk will have great significance in the full use of goats and the development of the goat milk industry in China. METHODS: The effect of fermentation temperature (35°C, 37°C, 39°C), strain ratio (1:1:1, 2:1:1, 3:1:1) and inoculum size (4%, 5%, 6%) on viable counts of L...
April 2016: Acta Scientiarum Polonorum. Technologia Alimentaria
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