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https://www.readbyqxmd.com/read/28453269/production-of-protein-concentrate-and-1-3-propanediol-by-wheat-based-thin-stillage-fermentation
#1
Kornsulee Ratanapariyanuch, Youn Young Shim, Shahram Emami, Martin John Tarsisius Reaney
Fermentation of wheat with yeast produces thin stillage (W-TS) and distiller's wet grains. A subsequent fermentation of W-TS (two-stage fermentation, TSF) with endemic bacteria at 25 and 37 °C decreased glycerol and lactic acid concentrations while 1,3-propanediol (1,3-PD) and acetic acid accumulated with greater 1,3-PD and acetic acid produced at 37 °C. During TSF, W-TS colloids coagulated and floated in the fermentation medium producing separable liquid and slurry fractions. The predominant endemic bacteria in W-TS were Lactobacillus panis, L...
April 28, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28446905/okara-a-nutritionally-valuable-by-product-able-to-stabilize-lactobacillus-plantarum-during-freeze-drying-spray-drying-and-storage
#2
Gabriel Quintana, Esteban Gerbino, Andrea Gómez-Zavaglia
Okara is a nutritionally valuable by-product produced in large quantities as result of soymilk elaboration. This work proposes its use as both culture and dehydration medium during freeze-drying, spray-drying, and storage of Lactobacillus plantarum CIDCA 83114. Whole and defatted okara were employed as culture media for L. plantarum CIDCA 83114. The growth kinetics were followed by plate counting and compared with those of bacteria grown in MRS broth (control). No significant differences in plate counting were observed in the three media...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28446769/identification-of-key-proteins-and-pathways-in-cadmium-tolerance-of-lactobacillus-plantarum-strains-by-proteomic-analysis
#3
Qixiao Zhai, Yue Xiao, Jianxin Zhao, Fengwei Tian, Hao Zhang, Arjan Narbad, Wei Chen
Our previous study confirmed the protective potential of Lactobacillus plantarum (L. plantarum) strains in alleviation of cadmium (Cd) toxicity in vivo and demonstrated that the observed protection largely depended on the tolerance of the strains to Cd-induced stress. It was also observed that there were significant intra-species differences in Cd tolerance of L. plantarum strains. In this study, we investigated the mechanism of Cd induced stress response of L. plantarum strains using the isobaric tags for relative and absolute quantitation (iTRAQ) based comparative proteomics...
April 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28445967/lactobacillus-casei-zhang-and-vitamin-k2-prevent-intestinal-tumorigenesis-in-mice-via-adiponectin-elevated-different-signaling-pathways
#4
Yong Zhang, Chen Ma, Jie Zhao, Haiyan Xu, Qiangchuan Hou, Heping Zhang
The incidence of colon cancer has increased considerably and the intestinal microbiota participate in the development of colon cancer. We showed that the L. casei Zhang or vitamin K2 (Menaquinone-7) intervention significantly alleviated intestinal tumor burden in mice. This was associated with increased serum adiponectin levels in both treatments. But osteocalcin level was only increased by L. casei Zhang. Furthermore, the anti-carcinogenic actions of L. casei Zhang were mediated by hepatic Chloride channel-3(CLCN3)/Nuclear Factor Kappa B(NF-κB) and intestinal Claudin15/Chloride intracellular channel 4(CLIC4)/Transforming Growth Factor Beta(TGF-β) signaling, while the vitamin K2 effect involved a hepatic Vitamin D Receptor(VDR)-phosphorylated AMPK signaling pathway...
April 11, 2017: Oncotarget
https://www.readbyqxmd.com/read/28444600/antibiotic-sensitivity-pattern-of-indigenous-lactobacilli-isolated-from-curd-and-human-milk-samples
#5
Chetan Sharma, Sachin Gulati, Nishchal Thakur, Brij Pal Singh, Sanjolly Gupta, Simranpreet Kaur, Santosh Kumar Mishra, Anil Kumar Puniya, Jatinder Pal Singh Gill, Harsh Panwar
The gut microbiota plays a vital role in host well-being and lactic acid bacteria (LAB) have gained an overwhelming attention as health promoter. This perception has evolved from traditional dairy products to a money-spinning market of probiotics. The safety of probiotics is coupled to their intended use and LAB may act as pool of antimicrobial resistance genes that could be transferred to pathogens, either in food matrix or in gastrointestinal tract, which could be detrimental to host. This study evaluated the antibiotic susceptibility patterns of LAB isolated from curd (20) and human milk (11) samples...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28444596/response-surface-design-for-accumulation-of-selenium-by-different-lactic-acid-bacteria
#6
Jingpeng Yang, Yao Li, Li Zhang, Mingtao Fan, Xinyuan Wei
The accumulation of selenium (Se) by Lactobacillus delbrueckii ssp. bulgaricus (Lb) and Streptococcus thermophilus (St) at the different cultivation conditions, including initial pH, inoculum dose (%), and temperature (°C), was investigated in this work. Se enrichment efficiency was optimized using the Design-Expert software for response surface methodology on a basis of single-factor experiment. The antioxidant activities of Se-enriched Lactic acid bacteria (LAB) were also investigated. The qualitative analysis of Se-enriched LAB was performed by FT-IR spectra...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28443847/the-impact-of-dietary-sn-2-palmitic-triacylglycerols-in-combination-with-docosahexaenoic-acid-or-arachidonic-acid-on-lipid-metabolism-and-host-faecal-microbiota-composition-in-sprague-dawley-rats
#7
Jianchun Wan, Songyou Hu, Jörg J Jacoby, Jie Liu, Yaqiong Zhang, Liangli Lucy Yu
Sn-2 palmitic acid triacylglycerols (sn2PA fat) and polyunsaturated fatty acids are thought to influence the metabolic status and intestinal bacterial population of the host. In this study, the impact of sn2PA fat in combination with DHA or ARA in the diet on lipid metabolism in the liver and faecal microbiota composition were investigated in rats fed diets containing sn2PA fat, 90% sn2PA fat + 10% DHA oil (wt%), or 90% sn2PA fat + 10% ARA oil (wt%). Tissue fatty acid composition was measured using gas chromatography (GC), whereas the faecal microbial composition was assessed using 16S rRNA high-throughput sequencing technology...
April 26, 2017: Food & Function
https://www.readbyqxmd.com/read/28443531/probiotics-to-prevent-necrotising-enterocolitis-and-nosocomial-infection-in-very-low-birth-weight-preterm-infants
#8
J Uberos, E Aguilera-Rodríguez, A Jerez-Calero, M Molina-Oya, A Molina-Carballo, E Narbona-López
The aim of the study was to determine whether routine probiotic supplementation (RPS) with Lactobacillus rhamnosus GG (LGG) or Lactobacillus acidophilus +Lactobacillus bifidum is associated with reduced risk of necrotising enterocolitis (NEC)≥Stage II in preterm neonates born at ≤32 weeks' gestation. We conducted a retrospective cohort study on the effect of probiotic supplementation in very low birth weight infants in our neonatal unit by comparing two periods: before and after supplementation. The incidence of NEC≥Stage II, late-onset sepsis and all-cause mortality was compared for an equal period 'before' (Period I) and 'after' (Period II) RPS with LGG or L...
April 26, 2017: British Journal of Nutrition
https://www.readbyqxmd.com/read/28443383/beneficial-effects-of-lactobacillus-casei-strain-shirota-on-academic-stress-induced-sleep-disturbance-in-healthy-adults-a-double-blind-randomised-placebo-controlled-trial
#9
M Takada, K Nishida, Y Gondo, H Kikuchi-Hayakawa, H Ishikawa, K Suda, M Kawai, R Hoshi, Y Kuwano, K Miyazaki, K Rokutan
The present study examined whether Lactobacillus casei strain Shirota (LcS) improves sleep quality under psychological stress. A double-blind, placebo-controlled trial was conducted in healthy 4(th) year medical students exposed to academic examination stress. The trial was repeated over two consecutive years in different groups of students, and the data were pooled. For 8 weeks prior to and 3 weeks after a national standardised examination, a total of 48 and 46 subjects received a daily dose of 100 ml of LcS-fermented milk or non-fermented placebo milk, respectively...
April 26, 2017: Beneficial Microbes
https://www.readbyqxmd.com/read/28443224/the-effect-of-lactobacillus-acidophilus-ptcc-1643-on-cultured-intestinal-epithelial-cells-infected-with-salmonella-enterica-serovar-enteritidis
#10
Mona Moshiri, Mohammad Mehdi Soltan Dallal, Farhad Rezaei, Masoumeh Douraghi, Laleh Sharifi, Zahra Noroozbabaei, Mehrdad Gholami, Abbas Mirshafiey
OBJECTIVES: Gastrointestinal disorders caused by Salmonella enterica serovar Enteritidis (SesE) are a significant health problem around the globe. Probiotic bacteria have been shown to have positive effects on the immune responses. Lactobacillus acidophilus was examined for its capability to influence the innate immune response of HT29 intestinal epithelial cells towards SesE. The purpose of this work was to assess the effect of L. acidophilus PTCC 1643 on cultured intestinal epithelial cells infected with SesE...
February 2017: Osong Public Health and Research Perspectives
https://www.readbyqxmd.com/read/28443073/feed-supplementation-with-red-seaweeds-chondrus-crispus-and-sarcodiotheca-gaudichaudii-reduce-salmonella-enteritidis-in-laying-hens
#11
Garima Kulshreshtha, Bruce Rathgeber, Janice MacIsaac, Martine Boulianne, Lehoux Brigitte, Glenn Stratton, Nikhil A Thomas, Alan T Critchley, Jeff Hafting, Balakrishnan Prithiviraj
Salmonella Enteritidis is vertically transmitted to eggs from laying hens through infected ovaries and oviducts. S. Enteritidis can also penetrate the eggshell from contaminated feces. Reducing S. Enteritidis in laying hens is vital to provide safer eggs and minimize the spread of salmonellosis to humans. Antibiotics have been widely used to control bacterial diseases in broilers and laying hens. However, there is a major concern that the use of antibiotics leads to the development of antibiotic resistance and adverse effects on microbiota of the treated birds...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28443071/deletion-of-lipoteichoic-acid-synthase-impacts-expression-of-genes-encoding-cell-surface-proteins-in-lactobacillus-acidophilus
#12
Kurt Selle, Yong J Goh, Brant R Johnson, Sarah O'Flaherty, Joakim M Andersen, Rodolphe Barrangou, Todd R Klaenhammer
Lactobacillus acidophilus NCFM is a well-characterized probiotic microorganism, supported by a decade of genomic and functional phenotypic investigations. L. acidophilus deficient in lipoteichoic acid (LTA), a major immunostimulant in Gram-positive bacteria, has been shown to shift immune system responses in animal disease models. However, the pleiotropic effects of removing LTA from the cell surface in lactobacilli are unknown. In this study, we surveyed the global transcriptional and extracellular protein profiles of two strains of L...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28442250/analysis-of-microbiota-in-first-episode-psychosis-identifies-preliminary-associations-with-symptom-severity-and-treatment-response
#13
Emanuel Schwarz, Johanna Maukonen, Tiina Hyytiäinen, Tuula Kieseppä, Matej Orešič, Sarven Sabunciyan, Outi Mantere, Maria Saarela, Robert Yolken, Jaana Suvisaari
The effects of gut microbiota on the central nervous system, along its possible role in mental disorders, have received increasing attention. Here we investigated differences in fecal microbiota between 28 patients with first-episode psychosis (FEP) and 16 healthy matched controls and explored whether such differences were associated with response after up to 12months of treatment. Numbers of Lactobacillus group bacteria were elevated in FEP-patients and significantly correlated with severity along different symptom domains...
April 22, 2017: Schizophrenia Research
https://www.readbyqxmd.com/read/28442158/diversity-and-evolutionary-aspects-of-mucin-binding-mucbp-domain-repeats-among-lactobacillus-plantarum-group-strains-through-comparative-genetic-analysis
#14
Sundru Manjulata Devi, Prakash M Halami
Mucin binding protein (Mub) of Lactobacillus plantarum- group (LPG) is considered as a potential marker gene due to its diversity and adherence property to host intestinal layer. We recently observed strain specific diversity in mub gene of LPG strains (n=8) and grouped them into Type I, II and III based on the insertion and/or deletion of MucBP domain repeats (Devi et al., 2016). The mub gene expression pattern noted by real-time PCR revealed 67.64 increased fold change in MTCC 9483 with four MucBP repeats...
April 12, 2017: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/28441885/lactobacillus-plantarum-l9-but-not-lactobacillus-acidophilus-la-reduces-tumour-necrosis-factor-induced-bacterial-translocation-in-caco-2-cells
#15
B Wang, J Chen, S Wang, X Zhao, G Lu, X Tang
Translocation of bacteria across the intestinal barrier is important in the pathogenesis of systemic sepsis and multiple organ dysfunction syndromes. Inflammatory cytokines increase paracellular permeability that allows increased luminal bacteria to translocate across mucosal epithelium and further deteriorate the gut barrier. In order to reduce this risk, the prophylactic use of probiotics has been recently addressed. In this paper, we investigate the protective role toward tumour necrosis factor (TNF)-α induced non-pathogenic Escherichia coli translocation across Caco-2 monolayers of Lactobacillus strains...
April 26, 2017: Beneficial Microbes
https://www.readbyqxmd.com/read/28440871/effects-of-different-salt-treatments-on-the-fermentation-metabolites-and-bacterial-profiles-of-kimchi
#16
Dong Wook Kim, Bo-Min Kim, Hyeon-Jeong Lee, Gwang-Ju Jang, Seong Hwa Song, Jae-In Lee, Sang Bong Lee, Jae Min Shim, Kang Wook Lee, Jeong Hwan Kim, Kyung-Sik Ham, Feng Chen, Hyun-Jin Kim
The effects of purified salt (PS) and mineral-rich sea salt (MRS), both with different mineral profiles, on kimchi fermentation were studied using a culture-dependent 16S rRNA sequencing technique and mass-based metabolomic analysis. The different mineral profiles in the fermentation medium caused changes in the bacterial profiles of the 2 kimchi products. An increase of Leuconostoc species in MRS-kimchi decreased the Lactobacillus/Leuconostoc ratio, which led to changes in metabolites (including sugars, amino acids, organic acids, lipids, sulfur compounds, and terpenoids) associated with kimchi quality...
April 25, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28439768/discrimination-of-wine-lactic-acid-bacteria-by-raman-spectroscopy
#17
Susan B Rodriguez, Mark A Thornton, Roy J Thornton
Species of Lactobacillus, Pediococcus, Oenococcus, and Leuconostoc play an important role in winemaking, as either inoculants or contaminants. The metabolic products of these lactic acid bacteria have considerable effects on the flavor, aroma, and texture of a wine. However, analysis of a wine's microflora, especially the bacteria, is rarely done unless spoilage becomes evident, and identification at the species or strain level is uncommon as the methods required are technically difficult and expensive. In this work, we used Raman spectral fingerprints to discriminate 19 strains of Lactobacillus, Pediococcus, and Oenococcus...
April 24, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28439679/screening-lactic-acid-bacteria-strains-with-ability-to-bind-di-n-butyl-phthalate-via-turbiscan-technique
#18
Zhao Lili, Zhao Hongfei, Sana Shoukat, Zhang Xiaochen, Zhang Bolin
Di-n-butyl phthalate (DBP) is a ubiquitous environmental contaminant that poses a risk to humans. Previous work indicates that the ability of lactic acid bacteria (LAB) to bind phthalic acid esters is strain-specific. As cell suspensions of LAB strains in aqueous solution are likely to be colloidal dispersions, this study provided a technique to efficiently screen LAB strains that bind DBP via Turbiscan, which has been widely used to measure the stability of emulsions or colloidal dispersions. Eleven LAB strains belonging to Lactobacillus plantarum, Lb...
April 24, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28439658/pan-genomic-approaches-in-lactobacillus-reuteri-as-a-porcine-probiotic-investigation-of-host-adaptation-and-antipathogenic-activity
#19
Jun-Yeong Lee, Geon Goo Han, Jaeyun Choi, Gwi-Deuk Jin, Sang-Kee Kang, Byung Jo Chae, Eun Bae Kim, Yun-Jaie Choi
After the introduction of a ban on the use of antibiotic growth promoters (AGPs) for livestock, reuterin-producing Lactobacillus reuteri is getting attention as an alternative to AGPs. In this study, we investigated genetic features of L. reuteri associated with host specificity and antipathogenic effect. We isolated 104 L. reuteri strains from porcine feces, and 16 strains, composed of eight strains exhibiting the higher antipathogenic effect (group HS) and eight strains exhibiting the lower effect (group LS), were selected for genomic comparison...
April 24, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/28439489/detection-and-analysis-of-lactobacillus-paracasei-penicillin-binding-proteins-revealed-the-presence-of-cholate-sensitive-penicillin-binding-protein-3-and-an-elongated-cell-shape-in-a-cholate-sensitive-strain
#20
Masahiro Hattori, Glaezel Angelique Torres, Naoto Tanaka, Sanae Okada, Akihito Endo, Junichi Nakagawa
Penicillin-binding proteins (PBPs) are responsible for peptidoglycan synthesis. By using biotinylated ampicillin, we detected PBPs of Lactobacillus paracasei strains. Ten PBPs were identified, 7 of which had apparent molecular sizes similar to those of Escherichia coli. In the presence of cholate, strain NRIC 0625 showed an elongated shape, and its putative PBP3 showed cholate-sensitive penicillin-binding activity. Furthermore, this strain was highly sensitive to cefalexin, which is known to inhibit cell division by inactivating PBP3...
2017: Bioscience of Microbiota, Food and Health
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