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https://www.readbyqxmd.com/read/28222388/microbial-profile-of-dental-plaque-associated-to-white-spot-lesions-in-orthodontic-patients-immediately-after-the-bracket-removal
#1
Moniek W Beerens, Jacob M Ten Cate, Monique H van der Veen
OBJECTIVE: Denaturing Gradient Gel Electrophoresis (DGGE) is suggested to predict caries risk in young children. Such a tool would be valuable in orthodontic patients undergoing treatment with fixed appliances. In this cross-sectional study the applicability of DGGE and conventional microbiology for caries risk assessment in orthodontic patients were assessed. DESIGN: Dental plaque was obtained from orthodontic patients immediately prior to bracket removal. Presence of white spot lesions (WSL) was assessed immediately post debracketing...
February 9, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28221938/reduction-of-biogenic-amines-during-miso-fermentation-by-lactobacillus-plantarum-as-a-starter-culture
#2
Yi-Chen Lee, Hsien-Feng Kung, Ya-Ling Huang, Chien-Hui Wu, Yu-Ru Huang, Yung-Hsiang Tsai
Lactobacillus plantarum D-103 isolated from a miso product that possesses amine-degrading activity was used as a starter culture in miso fermentation (25°C for 120 days) in this study. The salt content in control samples (without starter culture) and inoculated samples (inoculated with L. plantarum D-103) remained constant at 10.4% of the original salt concentration throughout fermentation, whereas the pH value decreased from 6.2 to 4.6 during fermentation. The inoculated samples had significantly lower (P < 0...
September 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221912/isolation-identification-and-evaluation-of-new-lactic-acid-bacteria-strains-with-both-cellular-antioxidant-and-bile-salt-hydrolase-activities-in-vitro
#3
Shuang Xu, Taigang Liu, Chiraz Akorede Ibinke Radji, Jing Yang, Lanming Chen
In this study, we analyzed Chinese traditional fermented food to isolate and identify new lactic acid bacteria (LAB) strains with novel functional properties and to evaluate their cellular antioxidant and bile salt hydrolase (BSH) activities in vitro. A sequential screening strategy was developed to efficiently isolate and obtain 261 LAB strains tolerant of bile salt, acid, and H2O2 from nine Chinese traditional fermented foods. Among these strains, 70 were identified as having 2,2-diphenyl-1-picrylhydrazyl radical scavenging and/or BSH activity...
November 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221876/effect-of-magnesium-acetate-on-the-antimold-activity-of-lactobacillus
#4
Katarzyna Kycia, Anna Bzducha-Wróbel, Karolina Kraśniewska, Anna Chlebowska-Śmigiel, Małgorzata Gniewosz
The antimold activity of lactic acid bacteria (LAB) is used in food biopreservation. The aim of this study was to evaluate the effect of magnesium acetate added to de Man Rogosa Sharpe (MRS) medium on the antimold activity of three LAB strains ( Lactobacillus plantarum , Lactobacillus brevis , and Lactobacillus fermentum ) against molds contaminating food ( Aspergillus oryzae , Aspergillus niger , Penicillium chrysogenum , Fusarium avenaceum , and Rhizopus arrhizus ) and their ability to produce organic acids (acetic acid, lactic acid, and phenyllactic acid)...
January 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28221841/tetracycline-resistance-patterns-of-lactobacillus-buchneri-group-strains
#5
Marlies Feichtinger, Sigrid Mayrhofer, Wolfgang Kneifel, Konrad J Domig
Lactobacilli are applied as starter cultures for controlled fermentation in the production of food and feed. Among other lactobacilli, members of the Lactobacillus buchneri group are used in fermented milk, wine, and silage. Most of the L. buchneri species used for the manufacturing of food or feed are already on the list for qualified presumption of safety status and are recommended as biological agents by the European Food Safety Authority. Consequently, new strains intended as food or feed additives do not require any additional safety check than confirming the absence of transferable antibiotic resistance determinants...
October 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28220237/microbial-fuel-cell-coupled-to-biohydrogen-reactor-a-feasible-technology-to-increase-energy-yield-from-cheese-whey
#6
J Wenzel, L Fuentes, A Cabezas, C Etchebehere
An important pollutant produced during the cheese making process is cheese whey which is a liquid by-product with high content of organic matter, composed mainly by lactose and proteins. Hydrogen can be produced from cheese whey by dark fermentation but, organic matter is not completely removed producing an effluent rich in volatile fatty acids. Here we demonstrate that this effluent can be further used to produce energy in microbial fuel cells. Moreover, current production was not feasible when using raw cheese whey directly to feed the microbial fuel cell...
February 20, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28219800/variations-in-gut-microbiota-and-fecal-metabolic-phenotype-associated-with-depression-by-16s-rrna-gene-sequencing-and-lc-ms-based-metabolomics
#7
Meng Yu, Hongmei Jia, Chao Zhou, Yong Yang, Yang Zhao, Maohua Yang, Zhongmei Zou
As a prevalent, life-threatening and highly recurrent psychiatric illness, depression is characterized by a wide range of pathological changes; however, its etiology remains incompletely understood. Accumulating evidence supports that gut microbiota affects not only gastrointestinal physiology but also central nervous system (CNS) function and behavior through the microbiota-gut-brain axis. To assess the impact of gut microbiota on fecal metabolic phenotype in depressive conditions, an integrated approach of 16S rRNA gene sequencing combined with ultra high-performance liquid chromatography-mass spectrometry (UHPLC-MS) based metabolomics was performed in chronic variable stress (CVS)-induced depression rat model...
February 10, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28218810/phytosterols-improve-immunity-and-exert-anti-inflammatory-activity-in-weaned-piglets
#8
Qiaoling Hu, Zhuo Zhao, Shenglin Fang, Yiwei Zhang, Jie Feng
BACKGROUND: Phytosterols (PS), plant-derived natural steroid compounds, are novel feed additives to regulate immune function and promote pig growth. This study was conducted to determine the effects of PS on the immune response of weaned piglets. RESULTS: One hundred and twenty crossbred (Duroc × Landrace × Yorkshire) piglets with an average initial weight of 9.58 ± 0.26 kg were randomly allotted to three treatments. Treatments consisted of a control, PS (0...
February 20, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28217694/probiotics-for-prevention-of-urinary-stones
#9
John C Lieske
BACKGROUND: Urinary supersaturation is one key determinant of calcium oxalate (CaOx) urinary stone formation, and urinary excretions of oxalate and citrate are two key determinants. Each is influenced by gastrointestinal processes. METHODS: Open label and randomized placebo studies have examined the effect of oral probiotic preparations on urinary supersaturation and oxalate excretion. Cross sectional studies in humans have studied the association of Oxalobacter formigenes colonization status and urinary oxalate excretion and prevalence of urinary stones...
January 2017: Annals of Translational Medicine
https://www.readbyqxmd.com/read/28215898/influence-of-lactobacillus-plantarum-on-yogurt-fermentation-properties-and-subsequent-changes-during-postfermentation-storage
#10
Changkun Li, Jihong Song, Lai-Yu Kwok, Jicheng Wang, Yan Dong, Haijing Yu, Qiangchuan Hou, Heping Zhang, Yongfu Chen
This study aimed to evaluate the influence of 9 Lactobacillus plantarum with broad-spectrum antibacterial activity on fermented milk, including changes to the fermentation characteristics (pH, titration acidity, and viable counts), texture profile, relative content of volatile compounds, and sensory evaluation during 28-d storage at 4°C. First, L. plantarum IMAU80106, IMAU10216, and IMAU70095 were selected as candidates for further study because of their excellent coagulation and proteolytic activities. Subsequently, these L...
February 16, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28215741/comparison-of-dietary-inclusion-of-commercial-and-fermented-soybean-meal-on-oxidative-status-and-non-specific-immune-responses-in-white-shrimp-litopenaeus-vannameis
#11
Yu-Hung Lin, Jia-Jinn Mui
This study compared the effects of partial replacement of fish meal (FM) protein with commercial soybean meal (SBM) or Lactobacillus spp. fermented SBM (FSBM) on the oxidative status and non-specific immune responses in white shrimps, Litopenaeus vannamei. A FM-based diet was used as the control. To prepare the two experimental diets, 25% of the FM protein was replaced with SBM and FSBM. Three experimental diets were fed to three groups of shrimps (initial wt: 0.63 ± 0.01 g) in a recirculating rearing system for 12 weeks...
February 16, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28213763/the-lactobacillus-rhamnosus-and-lactobacillus-fermentum-strains-from-human-biotopes-characterized-with-mlst-and-toxin-antitoxin-gene-polymorphism
#12
E U Poluektova, R A Yunes, M V Epiphanova, V S Orlova, V N Danilenko
The diversity of Lb. rhamnosus and Lb. fermentum strains isolated from feces, saliva, and the vaginal cavity of 18-22-year-old healthy women residing in central regions of the Russian Federation has been characterized. The results obtained using multilocus sequence typing were identical to those obtained with the analysis of genetic and genomic polymorphism in TA systems. Different as well as identical Lb. rhamnosus and Lb. fermentum sequence types (ST) were isolated from various parts of the body of the same person...
February 17, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28213032/applicability-of-a-colorimetric-method-for-evaluation-of-lactic-acid-bacteria-with-probiotic-properties
#13
Min Young Jung, Jina Lee, Boyeon Park, Hyelyeon Hwang, Sung-Oh Sohn, Se Hee Lee, Hyeong In Lim, Hae Woong Park, Jong-Hee Lee
Rapid colorimetric methods using various indicator reagents have been developed to monitor bacterial viability. Here, we examined the applicability of a method based on the reduction of resazurin or water-soluble tetrazolium salt-8 (WST-8) to screen lactic acid bacteria (LAB) for growth, tolerance against bile acid and low pH. The resazurin reduction test proved unsuitable for screening LAB such as Lactobacillus plantarum and Leuconostoc mesenteroides since it reacted with acid present in the cultures. LAB growth could be indirectly quantified by measuring WST-8 reduction...
June 2017: Food Microbiology
https://www.readbyqxmd.com/read/28212503/lactobacillus-buchneri-s-layer-as-carrier-for-an-ara-h-2-derived-peptide-for-peanut-allergen-specific-immunotherapy
#14
Julia Anzengruber, Merima Bublin, Eva Bönisch, Bettina Janesch, Angelika Tscheppe, Matthias L Braun, Eva-Maria Varga, Christine Hafner, Heimo Breiteneder, Christina Schäffer
Peanut allergy is an IgE-mediated severe hypersensitivity disorder. The lack of a treatment of this potentially fatal allergy has led to intensive research on vaccine development. Here, we describe the design and initial characterization of a carrier-bound peptide derived from the most potent peanut allergen, Ara h 2, as a candidate vaccine. Based on the adjuvant capability of bacterial surface (S-) layers, a fusion protein of the S-layer protein SlpB from Lactobacillus buchneri CD034 and the Ara h 2-derived peptide AH3a42 was produced...
February 14, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28210874/erratum-to-effect-of-lactobacillus-reuteri-on-cell-viability-and-pge2-production-in-human-gingival-fibroblasts
#15
Gina A Castiblanco, Tulay Yucel-Lindberg, Stefan Roos, Svante Twetman
No abstract text is available yet for this article.
February 16, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28210467/diversity-and-distribution-of-culturable-lactic-acid-bacterial-species-in-indonesian-sayur-asin
#16
Wibowo Mangunwardoyo, Abinawanto, Andi Salamah, Endang Sukara, Sulistiani, Achmad Dinoto
BACKGROUND AND OBJECTIVES: Lactic acid bacteria (LAB) play important roles in processing of Sayur Asin (spontaneously fermented mustard). Unfortunately, information about LAB in Indonesian Sayur Asin, prepared by traditional manufactures which is important as baseline data for maintenance of food quality and safety, is unclear. The aim of this study was to describe the diversity and distribution of culturable lactic acid bacteria in Sayur Asin of Indonesia. MATERIALS AND METHODS: Four Sayur Asin samples (fermentation liquor and fermented mustard) were collected at harvesting times (3-7 days after fermentation) from two traditional manufactures in Tulung Agung (TA) and Kediri (KDR), East Java provinces, Indonesia...
August 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28210256/transcriptomic-analysis-of-the-innate-antiviral-immune-response-in-porcine-intestinal-epithelial-cells-influence-of-immunobiotic-lactobacilli
#17
Leonardo Albarracin, Hisakazu Kobayashi, Hikaru Iida, Nana Sato, Tomonori Nochi, Hisashi Aso, Susana Salva, Susana Alvarez, Haruki Kitazawa, Julio Villena
Lactobacillus rhamnosus CRL1505 and Lactobacillus plantarum CRL1506 are immunobiotic strains able to increase protection against viral intestinal infections as demonstrated in animal models and humans. To gain insight into the host-immunobiotic interaction, the transcriptomic response of porcine intestinal epithelial (PIE) cells to the challenge with viral molecular associated pattern poly(I:C) and the changes in the transcriptomic profile induced by the immunobiotics strains CRL1505 and CRL1506 were investigated in this work...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28209827/first-complete-genome-sequence-of-marinilactibacilluspiezotolerans-strain-15r-a-marine-lactobacillus-isolated-from-coal-bearing-sediment-2-0-kilometers-below-the-seafloor-determined-by-pacbio-single-molecule-real-time-technology
#18
Yuli Wei, Junwei Cao, Jiasong Fang, Chiaki Kato, Weicheng Cui
Marinilactibacillus piezotolerans strain 15R is a facultatively anaerobic heterotrophic lactobacillus isolated from deep marine subsurface sediment nearly 2 km below the seafloor in the northwestern Pacific. We report here the first whole-genome sequence of strain 15R. The identified genome sequence has 2,767,908 bp, 35.4% G+C content, and predicted 2,552 candidate protein-coding sequences, with no identified plasmids.
February 16, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28207855/novel-approaches-for-the-taxonomic-and-metabolic-characterization-of-lactobacilli-integration-of-16s-rrna-gene-sequencing-with-maldi-tof-ms-and-1h-nmr
#19
Claudio Foschi, Luca Laghi, Carola Parolin, Barbara Giordani, Monica Compri, Roberto Cevenini, Antonella Marangoni, Beatrice Vitali
Lactobacilli represent a wide range of bacterial species with several implications for the human host. They play a crucial role in maintaining the ecological equilibrium of different biological niches and are essential for fermented food production and probiotic formulation. Despite the consensus about the 'health-promoting' significance of Lactobacillus genus, its genotypic and phenotypic characterization still poses several difficulties. The aim of this study was to assess the integration of different approaches, genotypic (16S rRNA gene sequencing), proteomic (MALDI-TOF MS) and metabolomic (1H-NMR), for the taxonomic and metabolic characterization of Lactobacillus species...
2017: PloS One
https://www.readbyqxmd.com/read/28207812/of-the-milk-sugars-galactose-but-not-prebiotic-galacto-oligosaccharide-improves-insulin-sensitivity-in-male-sprague-dawley-rats
#20
Priska Stahel, Julie J Kim, Changting Xiao, John P Cant
BACKGROUND: Consumption of dairy products reduces risk of type 2 diabetes. Milk proteins and fats exhibit anti-diabetic properties but milk sugars have been studied little in this context. Galactose from milk lactose is readily converted to glycogen in the liver but its effects on insulin sensitivity have not been assessed. Prebiotic oligosaccharides from milk alter gut microbiota and can thereby influence host metabolism. Our objective was to assess the effect on insulin sensitivity of dietary galactose compared to glucose and fructose, and fermentable galacto-oligosaccharides compared to non-fermentable methylcellulose...
2017: PloS One
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