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Zhaoxiang Yu, Xiaobo Lin, Ming Tian, Weiping Chang
Accumulating evidence indicates that microRNAs (miRNAs) play important roles in tumorigenesis and metastasis. Recent research has shown that miR‑196b is implicated in metastasis by regulating the migration and invasion of cancer cells. However, the clinical significance of miR‑196b and its role as well as the underlying mechanisms in hepatocellular carcinoma (HCC) remain largely unknown. Here, we detected miR‑196b expression in HCC and matched non-tumor tissues with qRT‑PCR. We found that miR‑196b displayed higher expression in HCC patient tissues and cells...
December 4, 2017: Oncology Reports
Hsiao-Wei Kao, Chao-Yu Pan, Chun-Hung Lai, Chew-Wun Wu, Wen-Liang Fang, Kuo-Hung Huang, Wen-Chang Lin
Many reports have implicated that microRNAs involve in cancer development and progression, such as miR-155 in breast cancers and miR-196 in gastric cancers. Furthermore, microRNAs are more stable than typical protein-coding gene mRNAs in varieties of clinical samples including body fluids. This suggests that they are potentially valuable biomarkers for cancer monitoring. In this study, we have used urine samples of gastric cancer patients to demonstrate the feasibility of urine microRNAs for gastric cancer detection...
August 22, 2017: Oncotarget
Hsiao-Wei Kao, Chao-Yu Pan, Chun-Hung Lai, Chew-Wun Wu, Wen-Liang Fang, Kuo-Hung Huang, Wen-Chang Lin
Many reports have implicated that microRNAs involve in cancer development and progression, such as miR-155 in breast cancers and miR-196 in gastric cancers. Furthermore, microRNAs are more stable than typical protein-coding gene mRNAs in varieties of clinical samples including body fluids. This suggests that they are potentially valuable biomarkers for cancer monitoring. In this study, we have used urine samples of gastric cancer patients to demonstrate the feasibility of urine microRNAs for gastric cancer detection...
April 7, 2017: Oncotarget
Jing Wen, Ying Huang, Hongying Li, Xupai Zhang, Peng Cheng, Donghong Deng, Zhigang Peng, Jun Luo, Weihua Zhao, Yongrong Lai, Zhenfang Liu
Myelodysplastic syndrome (MDS) is a clonal stem cell disorder characterized by ineffective hematopoiesis with a high risk of transformation to acute myeloid leukemia (AML). miRNAs function as tumor suppressors and oncogenes in various cancers and regulate the differentiation potential of hematopoietic stem and progenitor cells (HSPCs). It has been suggested that miRNAs may play an important role in progression of MDS. We analyzed bone marrow samples collected from MDS patients according to different risk stratification indicated by the International Prognostic Scoring System (IPSS)...
June 2017: International Journal of Hematology
Deivendran Rengaraj, Anh Duc Truong, Jihye Ban, Hyun S Lillehoj, Yeong Ho Hong
OBJECTIVE: Despite an increasing number of investigations into the pathophysiology of necrotic enteritis (NE) disease, etiology of NE-associated diseases, and gene expression profiling of NE-affected tissues, the microRNA (miRNA) profiles of NE-affected poultry have been poorly studied. The aim of this study was to induce NE disease in the genetically disparate Fayoumi chicken lines, and to perform non-coding RNA sequencing in the intestinal mucosal layer. METHODS: NE disease was induced in the Fayoumi chicken lines (M5...
July 2017: Asian-Australasian Journal of Animal Sciences
Zongze Shen, Xing Qin, Ming Yan, Rongrong Li, Gang Chen, Jianjun Zhang, Wantao Chen
Cancer-associated fibroblasts (CAFs), a major component of cancer stroma, play an important role in cancer progression but little is known about how CAFs affect tumorigenesis and development. MicroRNAs (miRNAs) are small non-coding RNAs that can negatively regulate target mRNA expression at post-transcriptional levels. In head and neck cancer (HNC), our analysis of miRNA arrays showed that miR-7, miR-196 and miR-335 were significantly up-regulated in CAFs when compared with their paired normal fibroblasts (NFs)...
January 3, 2017: Oncotarget
Songfei Shen, Jie Pan, Xingrong Lu, Pan Chi
The present study aimed to investigate the interaction between miR-196 and its target gene homeobox B8 (HoxB8) in colorectal cancer (CRC) cells, and the sensitivity of miR-196 and HoxB8 to fluorouracil, leucovorin and oxaliplatin (FOLFOX4) chemotherapy (1,200 mg/m(2) fluorouracil, 200 mg/m(2) leucovorin and 85 mg/m(2) oxaliplatin). In total, 80 tissue samples were collected in the present study. In total, 50 patients undergoing preoperative chemotherapy completed at least 3 cycles (2 weeks per cycle) of 85 mg/m(2) oxaliplatin (day 1) combined with a 2 h injection of 200 mg/m(2) leucovorin (days 1 and 2), a bolus injection of 400 mg/m(2) and 44 h continuous intravenous infusion of 1,200 mg/m(2) fluorouracil...
November 2016: Oncology Letters
Sung-Jae Baek, Katsutoshi Sato, Naohiro Nishida, Jun Koseki, Rikako Azuma, Koichi Kawamoto, Masamitsu Konno, Kazuhiko Hayashi, Taroh Satoh, Yuichiro Doki, Masaki Mori, Hideshi Ishii, Kazuhiko Ogawa
In this study, we compared the microRNA (miRNA) profiles of a control and X-ray- and carbon ion beam-resistant cells to identify miRNAs that can be used as radiosensitizers and biomarkers. Mouse squamous cell carcinoma line NR-S1, its X-ray-resistant derivative X60, and its carbon ion beam‑resistant derivative C30 were subjected to miRNA microarray analysis. Expression of miRNAs shown to be upregulated or downregulated in the microarray analysis was confirmed by qRT-PCR. Downregulated miRNAs were overexpressed in human pancreatic cancer cell lines PANC1 and MIA PaCa-2, and the resulting cells were tested for radiosensitivity using colony-forming and sphere-forming assays...
November 2016: Oncology Reports
Ying DU, Sheng Hua Yang, Sha Li, Chuan Jue Cui, Yan Zhang, Cheng Gang Zhu, Yuan Lin Guo, Na Qiong Wu, Ying Gao, Jing Sun, Qian Dong, Geng Liu, Jian Jun Li
OBJECTIVE: Very early-onset coronary artery disease (CAD) is a great challenge in cardiovascular medicine throughout the world, especially regarding its early diagnosis. This study explored whether circulating microRNAs (miRNAs) could be used as potential biomarkers for patients with very early-onset CAD. METHODS: We performed an initial screening of miRNA expression using RNA isolated from 20 patients with angiographically documented very early-onset CAD and 20 age- and sex-matched normal controls...
August 2016: Biomedical and Environmental Sciences: BES
Guanxiang Liang, Nilusha Malmuthuge, Yongjuan Guan, Yuwei Ren, Philip J Griebel, Le Luo Guan
The molecular regulatory mechanisms of host responses to Mycobacterium avium subsp. paratuberculosis (MAP) infection during the early subclinical stage are still not clear. In this study, surgically isolated ileal segments in newborn calves (n = 5) were used to establish in vivo MAP infection adjacent to an uninfected control intestinal compartment. RNA-Seq was used to profile the whole transcriptome (mRNAs) and the microRNAome (miRNAs) of ileal tissues collected at one-month post-infection. The most related function of the differentially expressed mRNAs between infected and uninfected tissues was "proliferation of endothelial cells", indicating that MAP infection may lead to the over-proliferation of endothelial cells...
April 22, 2016: Scientific Reports
Ya-Ching Lu, Joseph T Chang, Err-Cheng Chan, Yin-Kai Chao, Ta-Sen Yeh, Jinn-Shiun Chen, Ann-Joy Cheng
Over the past decade, the emergence of microRNA (miRNA) research has firmly established this molecular family as a key component in cells. MiRNAs, which function as negative gene regulators, participate in multiple biological processes and maintain homeostasis in cells. The dysregulation of miRNA may contribute to numerous human disorders, including cancer. Recently, miR-196 was found to be aberrantly expressed in a wide range of malignant diseases, which suggests that it plays important roles in carcinogenesis...
2016: Journal of Cancer
Yasmin G Hernandez, Aimee L Lucas
Pancreatic ductal adenocarcinoma (PDAC) is the 4(th) deadliest cancer in the United States, due to its aggressive nature, late detection, and resistance to chemotherapy. The majority of PDAC develops from 3 precursor lesions, pancreatic intraepithelial lesions (PanIN), intraductual papillary mucinous neoplasm (IPMN), and mucinous cystic neoplasm. Early detection and surgical resection can increase PDAC 5-year survival rate from 6% for Stage IV to 50% for Stage I. To date, there are no reliable biomarkers that can detect PDAC...
January 15, 2016: World Journal of Gastrointestinal Oncology
Jimmy Jacob, Rosy Favicchio, Negin Karimian, Maryam Mehrabi, Victoria Harding, Leandro Castellano, Justin Stebbing, Georgios Giamas
Lemur tyrosine kinase-3 (LMTK3) plays an important role in cancer progression and is associated with breast, lung, gastric and colorectal cancer. MicroRNAs (miRNAs) are small endogenous non-coding RNAs that typically repress target genes at post-transcriptional level and have an important role in tumorigenesis. By performing a miRNA expression profile, we identified a subset of miRNAs modulated by LMTK3. We show that LMTK3 induces miR-34a, miR-196-a2 and miR-182 levels by interacting with DEAD-box RNA helicase p68 (DDX5)...
March 1, 2016: Cancer Letters
Siew Fen Lisa Wong, Vikram Agarwal, Jennifer H Mansfield, Nicolas Denans, Matthew G Schwartz, Haydn M Prosser, Olivier Pourquié, David P Bartel, Clifford J Tabin, Edwina McGlinn
The Hox genes play a central role in patterning the embryonic anterior-to-posterior axis. An important function of Hox activity in vertebrates is the specification of different vertebral morphologies, with an additional role in axis elongation emerging. The miR-196 family of microRNAs (miRNAs) are predicted to extensively target Hox 3' UTRs, although the full extent to which miR-196 regulates Hox expression dynamics and influences mammalian development remains to be elucidated. Here we used an extensive allelic series of mouse knockouts to show that the miR-196 family of miRNAs is essential both for properly patterning vertebral identity at different axial levels and for modulating the total number of vertebrae...
September 1, 2015: Proceedings of the National Academy of Sciences of the United States of America
Sebastian Fantini, Valentina Salsi, Antonio Vitobello, Filippo M Rijli, Vincenzo Zappavigna
The miR-196 miRNA gene family located within the Hox gene clusters has been shown to function during embryogenesis and to be aberrantly expressed in various malignancies, including leukaemia, melanoma, and colorectal cancer. Despite its involvement in numerous biological processes, the control of miR-196 expression is still poorly defined. We identified the miR-196b promoter and found that the mature miR-196b originates from a large, non-coding primary transcript, which starts within an autonomous TATA box promoter and is not in physical continuity with either the Hoxa10 or Hoxa9 main primary transcripts...
August 2015: Biochimica et Biophysica Acta
Pavel Škrha, Aleš Hořínek, Michal Anděl, Jan Škrha
INTRODUCTION: Newly-onset diabetes mellitus (DM) in middle-aged and older people may be an early symptom of pancreatic cancer (PC). However, sensitive markers for PC are still missing. MicroRNAs (miRNAs) play an important role in a cell response regulation. Significant changes in miRNA expressions were observed in cancers. Our goal was to compare expressions of selected miRNAs in patients with PC, DM and controls. METHODS: We enrolled 74 patients with PC (42/32, with/without DM), 29 type 2 diabetic patients and 17 controls...
April 2015: Vnitr̆ní Lékar̆ství
Moein Amin, Alfred King-yin Lam
Aberrant expressions of micro-ribonucleic acids (miRs) are closely associated with the pathogenesis in many human cancers. In oesophageal adenocarcinomas, altered expressions of different sets of miRs are noted to be associated with the development of adenocarcinoma from Barrett's oesophagus. In different studies, miRs such as miR-192, miR-196 and miR-21 were frequently noted to up-regulated whereas miR-203, miR-205 and miR-let-7 were commonly down-regulated during the development of Barrett's oesophagus to oesophageal adenocarcinoma...
June 2015: Experimental and Molecular Pathology
Qian Xu, Ying Li, Yong-Fang Shang, Hui-Ling Wang, Min-Xiu Yao
AIM: To investigate the associations between miRNA-103 (miR-103) and insulin resistance and nonalcoholic fatty liver disease (NAFLD). METHODS: Serum samples were collected from 50 NAFLD patients who were overweight or obese (NAFLD group) and from 30 healthy subjects who served as controls (normal control group). Quantitative polymerase chain reaction was used to detect expression of miR-103. Fasting plasma glucose, fasting insulin, and triglyceride (TG) levels were measured...
January 14, 2015: World Journal of Gastroenterology: WJG
Olivia Candini, Carlotta Spano, Alba Murgia, Giulia Grisendi, Elena Veronesi, Maria Serena Piccinno, Manuela Ferracin, Massimo Negrini, Francesca Giacobbi, Franco Bambi, Edwin Mark Horwitz, Pierfranco Conte, Paolo Paolucci, Massimo Dominici
Human aging is associated with a decrease in tissue functions combined with a decline in stem cells frequency and activity followed by a loss of regenerative capacity. The molecular mechanisms behind this senescence remain largely obscure, precluding targeted approaches to counteract aging. Focusing on mesenchymal stromal/stem cells (MSC) as known adult progenitors, we identified a specific switch in miRNA expression during aging, revealing a miR-196a upregulation which was inversely correlated with MSC proliferation through HOXB7 targeting...
March 2015: Stem Cells
Ya-Ching Lu, Joseph T Chang, Chun-Ta Liao, Chung-Jan Kang, Shiang-Fu Huang, I-How Chen, Chi-Che Huang, Yu-Chen Huang, Wen-Ho Chen, Chi-Ying Tsai, Hung-Ming Wang, Tzu-Chen Yen, Guo-Rung You, Chang-Hsu Chiang, Ann-Joy Cheng
BACKGROUND: MicroRNA-196 (miR-196), which is highly up-regulated in oral cancer cells, has been reported to be aberrantly expressed in several cancers; however, the significance of miR-196 in oral cancer has not yet been addressed. METHODS: Cellular functions in response to miR-196 modulation were examined, including cell growth, migration, invasion and radio/chemosensitivity. Algorithm-based studies were used to identify the regulatory target of miR-196. The miR-196 target gene and downstream molecular mechanisms were confirmed by RT-qPCR, western blot, luciferase reporter and confocal microscopy analyses...
2014: Molecular Cancer
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