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https://www.readbyqxmd.com/read/28103229/protein-phosphatase-1-down-regulates-zyg-1-levels-to-limit-centriole-duplication
#1
Nina Peel, Jyoti Iyer, Anar Naik, Michael P Dougherty, Markus Decker, Kevin F O'Connell
In humans perturbations of centriole number are associated with tumorigenesis and microcephaly, therefore appropriate regulation of centriole duplication is critical. The C. elegans homolog of Plk4, ZYG-1, is required for centriole duplication, but our understanding of how ZYG-1 levels are regulated remains incomplete. We have identified the two PP1 orthologs, GSP-1 and GSP-2, and their regulators I-2SZY-2 and SDS-22 as key regulators of ZYG-1 protein levels. We find that down-regulation of PP1 activity either directly, or by mutation of szy-2 or sds-22 can rescue the loss of centriole duplication associated with a zyg-1 hypomorphic allele...
January 19, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28102831/biomarkers-in-adult-asthma-a-systematic-review-of-8-isoprostane-in-exhaled-breath-condensate
#2
Adam M Peel, Christina-Jane Crossman-Barnes, Jonathan Tang, Stephen Fowler, Gwyneth Davies, Andrew Wilson, Yoon Loke
OBJECTIVES: We aimed to assess the evidence for the use of 8-isoprostane in exhaled breath condensate (EBC) as a biomarker in adult asthma. DESIGN: A systematic review and meta-analysis of EBC 8-isoprostane. METHODS: We searched a number of online databases (including PubMed, Embase and Scopus) in January 2016. We included studies of adult non-smokers with EBC collection and asthma diagnosis conducted according to recognised guidelines. We aimed to pool data using random effects meta-analysis and assess heterogeneity using I2...
January 19, 2017: Journal of Breath Research
https://www.readbyqxmd.com/read/28101042/use-of-internal-limiting-membrane-autograft-in-primary-vitrectomy-for-rhegmatogenous-retinal-detachment
#3
Sergio Eustolio Hernández-Da Mota, Jorge Francisco Béjar-Cornejo
During a pars plana vitrectomy, an unplanned retinotomy in the raphe was performed in a 55-year-old female patient with rhegmatogenous retinal detachment and proliferative vitreoretinopathy. Since diathermy and laser therapy were not available at that moment, it was decided to peel off a small graft of the internal limiting membrane adjacent to the retinotomy site which had been previously stained with Brilliant Blue G. The graft was displaced under perfluorocarbon fluids and placed inside the retinotomy. Three weeks after surgery, the apparent closure of the retinotomy was observed clinically...
September 2016: Case Reports in Ophthalmology
https://www.readbyqxmd.com/read/28101038/successful-management-in-a-case-of-traumatic-retinal-detachment-due-to-open-globe-injury-using-microincisional-vitrectomy
#4
Wei-Yu Lai, Tsung-Tien Wu
BACKGROUND: Retinal detachment (RD) following ocular trauma often results in guarded visual prognosis and sometimes leads to loss of the eye. With the advent of microincisional vitrectomy surgery and the development of surgical techniques, the management of ocular trauma has been transformed. CASE PRESENTATION: A 34-year-old man sustained an open globe injury from fragmented glass at work. He received primary repair and another follow-up surgery 9 days later, including vitrectomy, silicone oil tamponade, and lensectomy for RD and traumatic cataract at another medical center...
September 2016: Case Reports in Ophthalmology
https://www.readbyqxmd.com/read/28098729/vitrectomy-for-persistent-macular-holes-following-ocriplasmin-injection-a-comparative-multicenter-study
#5
Ricarda G Schumann, Armin Wolf, Hans Hoerauf, Albrecht Lommatzsch, Mathias Maier, Joachim Wachtlin, Michael J Koss, Thomas Kreutzer, Thomas Bertelmann, Sara Kazerounian, Stefan Mennel, Siegfried G Priglinger
PURPOSE: To determine functional and anatomical outcomes of pars plana vitrectomy for persistent full-thickness macular hole (MH) after intravitreal injection of ocriplasmin. METHODS: This is a multicenter retrospective interventional study of 37 eyes of 37 patients who underwent pars plana vitrectomy with internal limiting membrane peeling for persistent MH after ocriplasmin treatment between December 2013 and December 2015 and comparison with 35 eyes of 35 patients who were offered ocriplasmin injection but underwent pars plana vitrectomy alone without pharmacologic vitreolysis before surgery...
January 16, 2017: Retina
https://www.readbyqxmd.com/read/28097306/universal-trichloroacetic-acid-peel-technique-for-light-and-dark-skin
#6
Nabil Fanous, Shadi Zari
Importance: Despite their great potential, medium and deep trichloroacetic acid peels are underused in light-skinned patients and are rarely used in darker-skinned patients because of the widespread fear of pigmentary complications and scarring. This concern has led many physicians to opt for the use of lighter types of peels (glycolic acid peel, Jessner peel, etc) and different lasers and intense light technologies. Trichloroacetic acid peels have been described in numerous publications...
January 12, 2017: JAMA Facial Plastic Surgery
https://www.readbyqxmd.com/read/28096983/pde3-inhibition-with-enoximone-as-first-line-therapy-for-severe-persistent-pulmonary-hypertension-of-the-newborn-during-neonatal-transport-a-case-report
#7
Robin van der Lee, Barbara Peels, Corine Koopman-Esseboom
Severe Persistent pulmonary hypertension of the newborn (PPHN) can be effectively treated with a PDE3 inhibitor as first-line treatment during neonatal transport when iNO is not readily available. Starting iNO as soon as possible is strongly advised because of the complementary actions of both therapeutics.
January 2017: Clinical Case Reports
https://www.readbyqxmd.com/read/28095556/combination-therapy-for-acne-scarring-personal-experience-and-clinical-suggestions
#8
Lucija Kroepfl, Jason J Emer
Acne is one of the most prevalent skin conditions seen by dermatologists. The cosmetic sequelae of severe acne, including scarring and pigmentation, have a profound psychological impact on those in icted. Topical (eg, retinoids, antibiotics, dapsone, hydroxyacids) and oral treatments (eg, antibiotics and/or spironolactone) are often bene cial to control acne or in the instance of oral isotretinoin use, rid the acne permanently; however, these treatments have very little affect on the ultimate cosmetic outcome of the acne scarring and skin texture that results...
November 1, 2016: Journal of Drugs in Dermatology: JDD
https://www.readbyqxmd.com/read/28095088/modeling-longitudinal-relationships-between-habit-and-physical-activity-two-cross-lagged-panel-design-studies-in-older-adults
#9
Rob J H van Bree, Catherine Bolman, Aart N Mudde, Maartje M van Stralen, Denise A Peels, Hein de Vries, Lilian Lechner
These longitudinal studies in older adults targeted mediated relationships between habit and physical activity (PA). In The Netherlands two independent studies were conducted among 1976 (Study 1: Mage=63.63, SD=8.66, 30% functional limitations) and 2140 (Study 2: Mage=62.75, SD=8.57, 45% functional limitations) adults aged 50 years or older. Cross-lagged panel designs were applied to examine whether habit mediates the relationship between prior and later PA and whether PA simultaneously mediates the relationship between prior and later habit...
January 17, 2017: Journal of Aging and Physical Activity
https://www.readbyqxmd.com/read/28089219/prognostic-factors-of-epiretinal-membranes-a-systematic-review
#10
REVIEW
A I M Miguel, A Legris
INTRODUCTION: Epiretinal membranes (ERM) have been increasingly characterized with the advent of new optical coherence tomographies (OCTs). We intended to perform a systematic review regarding prognostic factors (PF) of ERM after surgery. MATERIALS AND METHODS: Systematic review of electronic databases was performed (last date of search was 10 August 2015): Medline, Scopus, Google Scholar, clinicaltrials.gov and current controlled trials. Search queries included: "membrane", "pucker", "prognosis", "prognostic", "epiretinal", "épirétinienne"...
January 11, 2017: Journal Français D'ophtalmologie
https://www.readbyqxmd.com/read/28089066/evaluation-and-prevention-of-the-negative-matrix-effect-of-terpenoids-on-pesticides-in-apples-quantification-by-gas-chromatography-tandem-mass-spectrometry
#11
Géraldine Giacinti, Christine Raynaud, Sophie Capblancq, Valérie Simon
The sample matrix can enhance the gas chromatography signal of pesticide residues relative to that obtained with the same concentration of pesticide in solvent. This paper is related to negative matrix effects observed in coupled gas chromatography-mass spectrometry ion trap (GC/MS(2)) quantification of pesticides in concentrated extracts of apple peel prepared by the Quick Easy Cheap Effective Rugged and Safe (QuEChERS) method. It is focused on the pesticides most frequently used on the apple varieties studied, throughout the crop cycle, right up to harvest, to combat pests and diseases and to improve fruit storage properties...
December 21, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28088087/ethylene-biosynthesis-and-perception-during-ripening-of-loquat-fruit-eriobotrya-japonica-lindl
#12
E Alos, A Martinez-Fuentes, C Reig, C Mesejo, M J Rodrigo, M Agustí, L Zacarías
In order to gain insights into the controversial ripening behavior of loquat fruits, in the present study we have analyzed the expression of three genes related to ethylene biosynthesis (ACS1, ACO1 and ACO2), two ethylene receptors (ERS1a and ERS1b), one signal transduction component (CTR1) and one transcription factor (EIL1) in peel and pulp of loquat fruit during natural ripening and also in fruits treated with ethylene (10μLL(-1)) and 1-MCP (10μLL(-1)), an ethylene action inhibitor. In fruits attached to or detached from the tree, a slight increase in ethylene production was detected at the yellow stage, but the respiration rate declined progressively during ripening...
December 19, 2016: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28086814/intestinal-helminth-co-infection-and-associated-factors-among-tuberculosis-patients-in-arba-minch-ethiopia
#13
Getaneh Alemu, Mohammedaman Mama
BACKGROUND: Helminths affect the outcome of tuberculosis by shifting cell mediated immune response to humoral and by total suppression of the host immune system. On the reverse, Mycobacterium infection favors immune escape of helminths. Therefore assessing helminth co-infection rate and predisposing factors in tuberculosis patients is mandatory to set strategies for better case management. METHODS: Facility based cross-sectional study was conducted in Arba Minch to assess the prevalence and associated factors of intestinal helminths among pulmonary tuberculosis patients from January to August, 2016...
January 13, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28079613/centripetal-movement-of-the-capillaries-in-the-central-macular-region-after-internal-limiting-membrane-peeling
#14
Kazuyuki Kumagai, Masanori Hangai, Mariko Furukawa, Tetsuyuki Suetsugu, Nobuchika Ogino
PURPOSE: To report a case that showed centripetal movements of the capillaries in the central macular region after vitrectomy with internal limiting membrane peeling. METHODS: A 57-year-old pseudophakic woman underwent successful vitrectomy with internal limiting membrane peeling and air tamponade for a vitreomacular traction. Optical coherence tomography angiographic images of the 3 mm × 3 mm inner retinal vascular plexus were examined preoperatively and at 3 months postoperatively...
January 11, 2017: Retinal Cases & Brief Reports
https://www.readbyqxmd.com/read/28079592/implementation-science-the-laboratory-as-a-command-centre
#15
Debrah I Boeras, John N Nkengasong, Rosanna W Peeling
PURPOSE OF REVIEW: Recent advances in point-of-care technologies to ensure universal access to affordable quality-assured diagnostics have the potential to transform patient management, surveillance programmes, and control of infectious diseases. Decentralization of testing can put tremendous stresses on fragile health systems if the laboratory is not involved in the planning, introduction, and scale-up strategies. RECENT FINDINGS: The impact of investments in novel technologies can only be realized if these tests are evaluated, adopted, and scaled up within the healthcare system with appropriate planning and understanding of the local contexts in which these technologies will be used...
January 11, 2017: Current Opinion in HIV and AIDS
https://www.readbyqxmd.com/read/28077027/probabilistic-acute-risk-assessment-of-cumulative-exposure-to-organophosphorus-and-carbamate-pesticides-from-dietary-vegetables-and-fruits-in-shanghai-populations
#16
Fan Li, Yaqun Yuan, Pai Meng, Min Wu, Shuguang Li, Bo Chen
Organophosphorus (OPs) and carbamate pesticides (CPs) are among the most widely used pesticides in China, playing a major role in protecting agricultural commodities. In this study, we determined the cumulative acute exposure to organophosphate and carbamate pesticides of Shanghai residents from vegetables and fruits (VFs). The food consumption data were obtained from the Shanghai Food Consumption Survey (SHFCS) of 2012-2014 including a total of 1973 participants aged 2-90 years. The pesticide residue data were obtained from the Shanghai monitoring program during 2008-2011 with 34 organophosphates and 11 carbamates analyzed in a total of 5335 samples of VFs...
January 12, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28075566/chilling-stress-upregulates-%C3%AE-linolenic-acid-oxidation-pathway-and-induces-volatiles-of-c6-and-c9-aldehydes-in-mango-fruit
#17
Velu Sivankalyani, Itay Maoz, Oleg Feygenberg, Dalia Maurer, Noam Alkan
Mango-fruit storage period and shelf life are prolonged by cold storage. However, chilling temperature induces physiological and molecular changes, compromising fruit quality. In our previous transcriptomic study of mango fruit, cold storage at suboptimal temperature (5 °C) activated the α-linolenic acid metabolic pathway. To evaluate changes in fruit quality during chilling, we analyzed mango "Keitt" fruit peel volatiles. GC-MS analysis revealed significant modulations in fruit volatiles during storage at suboptimal temperature...
January 11, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28073278/wear-resistance-limited-by-step-edge-failure-the-rise-and-fall-of-graphene-as-an-atomically-thin-lubricating-material
#18
Yizhou Qi, Jun Liu, Ji Zhang, Yalin Dong, Qunyang Li
Owing to its intrinsically lubricious property, graphene has a high potential to be an atomically thin solid lubricant for sliding interfaces. Despite its ultrahigh breaking strength at the nanoscale, graphene often fails to maintain its integrity when subjected to macroscale tribological tests. To reveal the true wear characteristics of graphene, a nanoscale diamond tip was used to scratch monolayer graphene mechanically exfoliated to SiO2 substrates. Our experimental results show that while graphene exhibited extraordinary wear resistance in the interior region, it could be easily damaged at the step edge under a much lower normal load (∼2 orders of magnitude smaller)...
January 11, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28071019/influence-of-different-extraction-conditions-on-antioxidant-properties-of-soursop-peel
#19
Wei Zin Lee, Sui Kiat Chang, Hock Eng Khoo, Chiaw Mei Sia, Hip Seng Yim
BACKGROUND: Soursop is a healthy fruit. Peels form about 20% of the soursop fruit and are usually discarded as waste product. With a view to utilizing soursop peel as a source of valuable compounds, this study aimed to investigate the influence of different extraction conditions on total phenolic content (TPC) and antioxidant capacity (AC) of soursop (Annona muricata L.) peel. METHODS: Different ethanol concentrations (20-100%, v/v), extraction temperatures (25- 60°C), and extraction time (1-5 h) were tested...
October 2016: Acta Scientiarum Polonorum. Technologia Alimentaria
https://www.readbyqxmd.com/read/28070259/phenol-removal-from-aqueous-environment-by-adsorption-onto-pomegranate-peel-carbon
#20
Mojtaba Afsharnia, Mahdi Saeidi, Amin Zarei, Mohammad Reza Narooie, Hamed Biglari
INTRODUCTION: Phenol and its derivatives are the most common poisonous compounds which are stable in aqueous media and lead to many health issues. In this study, application of the carbon resulted from pomegranate peel is investigated in removal of phenol by adsorption method. METHODS: to perform this cross-sectional study, first, samples of phenol with concentrations of 10 to 100 mg/L were prepared for six months in 2016. Then, the impacts of parameters such as pH, adsorbent dosage, contact time, and initial concentration of phenol in adsorption process were investigated independently in Gonabad Chemistry Lab using a spectrophotometer at 505 nm as the wavelength...
November 2016: Electronic Physician
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