keyword
MENU ▼
Read by QxMD icon Read
search

Stromal

keyword
https://www.readbyqxmd.com/read/29793088/decellularized-and-matured-esophageal-scaffold-for-circumferential-esophagus-replacement-proof-of-concept-in-a-pig-model
#1
Guillaume Luc, Guillaume Charles, Caroline Gronnier, Magali Cabau, Charlotte Kalisky, Mallory Meulle, Reine Bareille, Samantha Roques, Lionel Couraud, Johanna Rannou, Laurence Bordenave, Denis Collet, Marlène Durand
Surgical resection of the esophagus requires sacrificing a long portion of it. Its replacement by the demanding gastric pull-up or colonic interposition techniques may be avoided by using short biologic scaffolds composed of decellularized matrix (DM). The aim of this study was to prepare, characterize, and assess the in vivo remodeling of DM and its clinical impact in a preclinical model. A dynamic chemical and enzymatic decellularization protocol of porcine esophagus was set up and optimized. The resulting DM was mechanically and biologically characterized by DNA quantification, histology, and histomorphometry techniques...
May 17, 2018: Biomaterials
https://www.readbyqxmd.com/read/29791894/highly-tumorigenic-diffuse-large-b-cell-lymphoma-cells-are-produced-by-coculture-with-stromal-cells
#2
Zhiguang Lin, Bobin Chen, Ting Wu, Xiaoping Xu
BACKGROUND/AIMS: Diffuse large B cell lymphoma (DLBCL) is heterogeneous. We aimed to explore how tumor microenvironment promotes lymphoma cell aggressiveness and heterogeneity. METHODS: We created a coculture system using human DLBCL cells and mouse bone marrow stromal cells. Proliferative capacity, drug resistance, clonogenicity, and tumorigenicity were compared in lymphoma cells from the coculture system and lymphoma cells cultured alone. Expression of Notch signaling associated genes was evaluated using real-time reverse transcriptase PCR and Western blot...
May 23, 2018: Acta Haematologica
https://www.readbyqxmd.com/read/29791034/high-expression-of-cd10-in-anaplastic-thyroid-carcinomas
#3
Tadao Nakazawa, Tetsuo Kondo, Huy Gia Vunong, Toru Odate, Masataka Kawai, Ippei Tahara, Kazunari Kasai, Tomohiro Inoue, Naoki Oishi, Kunio Mochizuki, Koichi Ito, Ryohei Katoh
AIMS: CD10 is an endopeptidase that degrades variable bioactive peptides in extra-cellular matrix. In addition to the enzymatic degradation, it affects multiple intra-cellular signal transduction pathways. CD10 expression has been extensively studied in human epithelial cancers of numerous organs and sites. However, its presence is not fully determined in thyroid carcinomas, especially in anaplastic thyroid carcinoma (ATC). METHODS AND RESULTS: We examined CD10 by immunohistochemistry (IHC) in 152 thyroid lesions: 9 adenomatous goiters (AGs) and 143 tumors including 47 anaplastic carcinomas...
May 23, 2018: Histopathology
https://www.readbyqxmd.com/read/29790424/the-fate-of-autologous-endometrial-mesenchymal-stromal-cells-after-application-in-the-healthy-equine-uterus
#4
Elisabeth Rink, Teresa Beyer, Hilari French, Elaine Watson, Christine Aurich, Xavier Donadeu
Because of their distinct differentiation, immunomodulatory and migratory capacities, endometrial mesenchymal stromal cells (MSCs) may provide an optimum source of therapeutic cells not only in relation to the uterus but also for regeneration of other tissues. This study reports the fate of endometrial MSCs following intrauterine application in mares. Stromal cell fractions were isolated from endometrial biopsies taken from seven reproductively healthy mares, expanded and fluorescence-labeled in culture. MSCs (15 x 106) or PBS were autologously infused into each uterine horn during early diestrus and subsequently tracked by fluorescence microscopy and flow cytometry of endometrial biopsies and blood samples taken periodically after infusion...
May 23, 2018: Stem Cells and Development
https://www.readbyqxmd.com/read/29790378/effect-of-hwnt10b-transgene-overexpression-on-peri-implant-osteogenesis-in-ovariectomized-rats
#5
Hanghang Liu, Nian Zhang, Yao Liu, Li Liu, Guozhu Yin, En Luo
Purpose This study aims to investigate the efficacy of human Wnt10b (hWnt10b) transgene in accelerating osseointegration around titanium implant in ovariectomized (OVX) rats, and to provide a new strategy for implant treatment of osteoporotic patients. Methods In vivo, osteoporosis rat model was constructed by bilateral ovariectomy and the expression change of Wnt pathway related genes was investigated. Then Ad-hWnt10b was locally administered to the femur defect prior to implant placement in OVX rats, surrounding femur tissues were collected 1 and 3 weeks respectively after implantation for imaging, biomechanical test, molecular and histological analysis...
May 23, 2018: Human Gene Therapy
https://www.readbyqxmd.com/read/29789905/pre-operative-mri-staging-of-endometrial-cancer-in-a-multicentre-cancer-network-can-we-match-single-centre-study-results
#6
N D Soneji, N Bharwani, A Ferri, V Stewart, A Rockall
OBJECTIVES: To evaluate the staging accuracy of magnetic resonance imaging (MRI) for endometrial cancer in daily practice over a 3-year period at a tertiary referral centre receiving scans from a large number of hospitals with varying protocols. To compare these daily practice results to published data from single-centre studies. METHODS: After ethical approval, MRI staging records for 270 studies from nine network and three centre hospitals were retrospectively collected and compared with final operative histopathology...
May 22, 2018: European Radiology
https://www.readbyqxmd.com/read/29789715/shared-and-independent-functions-of-apkc%C3%AE-and-par3-in-skin-tumorigenesis
#7
Susanne Vorhagen, Dominik Kleefisch, Oana-Diana Persa, Annika Graband, Alexandra Schwickert, Michael Saynisch, Michael Leitges, Carien M Niessen, Sandra Iden
The polarity proteins Par3 and aPKC are key regulators of processes altered in cancer. Par3/aPKC are thought to dynamically interact with Par6 but increasing evidence suggests that aPKC and Par3 also exert complex-independent functions. Whereas aPKCλ serves as tumor promotor, Par3 can either promote or suppress tumorigenesis. Here we asked whether and how Par3 and aPKCλ genetically interact to control two-stage skin carcinogenesis. Epidermal loss of Par3, aPKCλ, or both, strongly reduced tumor multiplicity and increased latency but inhibited invasion to similar extents, indicating that Par3 and aPKCλ function as a complex to promote tumorigenesis...
May 23, 2018: Oncogene
https://www.readbyqxmd.com/read/29789685/s100a4-protects-mice-from-high-fat-diet-induced-obesity-and-inflammation
#8
Shasha Hou, Ying Jiao, Qi Yuan, Junfeng Zhai, Tian Tian, Kaiji Sun, Zhinan Chen, Zhenlong Wu, Jinhua Zhang
As a member from S100 calcium-binding protein family, S100A4 is ubiquitous and elevated in tumor progression and metastasis, but its role in regulating obesity has not been well characterized. In this study, we showed that S100A4 was mainly expressed by stromal cells in adipose tissue and the S100A4 level in adipose tissue was decreased after high-fat diet (HFD). S100A4 deficient mice exhibited aggravated symptoms of obesity and suppressed insulin signaling after 12 weeks of HFD. Aggravated obesity in S100A4 deficient mice were found to be positively correlated with higher inflammatory status of the liver...
May 22, 2018: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/29789158/rap1-promotes-proliferation-and-migration-of-vascular-smooth-muscle-cell-via-the-erk-pathway
#9
Qin Li, Yunfei Teng, Jian Wang, Miao Yu, Yiqing Li, Hong Zheng
BACKGROUND: Rap1 is involved in a multitude of cellular signal transduction pathways, which has extensively been linked to cell proliferation and migration. It has been shown to be important in the regulation of physiological and pathological processes. The present study aims to elucidate its detailed mechanistic in proliferation and migration. MATERIAL/METHODS: Vascular smooth muscle cells (VSMCs) were transfected with pcDNA3.1(empty vector), pcDNA3.1 containing Myc-Tagged-Rap1V12 (Rap1V12) or pcDNA3...
April 17, 2018: Pathology, Research and Practice
https://www.readbyqxmd.com/read/29789013/prognostic-significance-of-pd-l1-expression-and-cd8-t-cell-infiltration-in-pulmonary-neuroendocrine-tumors
#10
Haiyue Wang, Zhongwu Li, Bin Dong, Wei Sun, Xin Yang, Ruping Liu, Lixin Zhou, Xiaozheng Huang, Ling Jia, Dongmei Lin
BACKGROUND: Recent research supports a significant role of immune checkpoint inhibitors in the treatment of solid tumors. However, relevant reports for programmed death-ligand 1 (PD-L1) and CD8+ tumor-infiltrating lymphocytes (TILs) in pulmonary neuroendocrine tumors (PNETs) have not been fully studied. Therefore, we investigated PNETs for the expression of PD-L1 and infiltration by CD8+ TILs as well as the prognostic value of both factors. METHODS: In total, 159 specimens of PNETs (35 TC, 2 AC, 28 LCNEC, 94 SCLC) were included in this study...
May 22, 2018: Diagnostic Pathology
https://www.readbyqxmd.com/read/29788742/mir-483-is-down-regulated-in-gastric-cancer-and-suppresses-cell-proliferation-invasion-and-protein-o-glcnacylation-by-targeting-ogt
#11
F Y Yu, C Y Zhou, Y B Liu, B Wang, L Mao, Y Li
MicroRNAs (miRNAs) are involved in the gastric carcinogenesis and progression. Here, we confirmed that miR-483 was frequently decreased in gastric cancer patients. The expression levels of miR-483 were negatively correlated with tumor stage, node metastasis and stromal invasion. Log-rank tests demonstrated that low expression of miR-483 was strongly correlated with poor overall survival in patients with gastric cancer. Moreover, ectopic expression of miR-483 remarkably suppressed gastric cancer cell proliferation by enhancing cell apoptosis and significantly inhibited the invasion of gastric cancer cells, while low expression of miR-483 exhibited the opposite effect...
March 14, 2018: Neoplasma
https://www.readbyqxmd.com/read/29788434/a-novel-mouse-model-of-testicular-granulosa-cell-tumors
#12
Xin Fang, Nan Ni, Yang Gao, David F Vincent, Laurent Bartholin, Qinglei Li
STUDY QUESTION: What is the role of dysregulated transforming growth factor beta (TGFB) signaling in the development of sex cord-stromal tumors in the testis? SUMMARY ANSWER: Overactivation of TGFB signaling results in the development of testicular tumors resembling granulosa cell tumors (GrCTs). WHAT IS KNOWN ALREADY: In an earlier study, we demonstrated that constitutively active TGFB receptor 1 (TGFBR1) in ovarian somatic cells promotes the development of ovarian GrCTs...
May 21, 2018: Molecular Human Reproduction
https://www.readbyqxmd.com/read/29788108/porphyromonas-gingivalis-strain-dependent-inhibition-of-uterine-spiral-artery-remodeling-in-the-pregnant-rat
#13
P Phillips, M B Brown, A Progulske-Fox, X J Wu, L Reyes
Porphyromonas gingivalis (Pg), is an important periodontal pathogen that is also implicated in pregnancy complications involving defective deep placentation (DDP). We hypothesized that Pg invasion of the placental bed promotes DDP. Pregnant rats were intravenously inoculated with sterile vehicle, Pg strain W83, or A7436 at gestation day (GD) 14 (acute cohort). Non-pregnant rats received repeated oral inoculations for 3 months before breeding (chronic cohort). Tissues and/or sera were collected at GD18 for analysis...
May 18, 2018: Biology of Reproduction
https://www.readbyqxmd.com/read/29788035/a-bayesian-hidden-potts-mixture-model-for-analyzing-lung-cancer-pathology-images
#14
Qiwei Li, Xinlei Wang, Faming Liang, Faliu Yi, Yang Xie, Adi Gazdar, Guanghua Xiao
Digital pathology imaging of tumor tissues, which captures histological details in high resolution, is fast becoming a routine clinical procedure. Recent developments in deep-learning methods have enabled the identification, characterization, and classification of individual cells from pathology images analysis at a large scale. This creates new opportunities to study the spatial patterns of and interactions among different types of cells. Reliable statistical approaches to modeling such spatial patterns and interactions can provide insight into tumor progression and shed light on the biological mechanisms of cancer...
May 18, 2018: Biostatistics
https://www.readbyqxmd.com/read/29787599/pathological-molecular-mechanism-of-symptomatic-late-onset-fuchs-endothelial-corneal-dystrophy-by-bioinformatic-analysis
#15
Zekai Cui, Qiaolang Zeng, Yonglong Guo, Shiwei Liu, Peiyuan Wang, Mengyuan Xie, Jiansu Chen
Fuchs endothelial corneal dystrophy (FECD) is a degenerative disease characterized by corneal endothelial decompensation. FECD causes corneal stromal and epithelial edema and progressively develops into bullous keratopathy, which can eventually lead to blindness. However, the exact pathogenesis is unknown. In this study, we performed an in-depth bioinformatic analysis of the dataset GSE74123 to determine the differentially expressed genes (DEGs) of symptomatic late-onset FECD compared with a normal control...
2018: PloS One
https://www.readbyqxmd.com/read/29787429/first-insights-into-the-m2-inflammatory-response-after-adipose-tissue-derived-stem-cell-injections-in-radiation-injured-muscles
#16
Diane Riccobono, Krisztina Nikovics, Sabine François, Anne-Laure Favier, Nicolas Jullien, Gerrit Schrock, Harry Scherthan, Michel Drouet
The cutaneous radiation syndrome is the clinical consequence of local high-dose irradiation. It is characterized by extensive inflammation, necrosis, and poor revascularization of the skin, resulting in muscle inflammation and fibrosis. Based on these physiopathological processes, subcutaneous injections of adipose-tissue-derived stem/stromal cells have shown favorable effects on skin-wound healing in a minipig model of cutaneous radiation syndrome, in which muscle fibrosis persisted. Since fibrosis is mainly due to the inflammatory processes that often affect underlying tissues as well, the beneficial effects of intramuscular injections of adipose-tissue-derived stem/stromal cells on tissue recovery were evaluated...
July 2018: Health Physics
https://www.readbyqxmd.com/read/29787037/effects-of-perinatal-exposure-to-dibutyltin-chloride-on-fat-and-glucose-metabolism-in-mice-and-molecular-mechanisms-in-vitro
#17
Raquel Chamorro-García, Bassem M Shoucri, Sigal Willner, Heidi Käch, Amanda Janesick, Bruce Blumberg
BACKGROUND: The organotin dibutyltin (DBT) is used in the manufacture of polyvinyl chloride (PVC) plastics, in construction materials, and in medical devices. Previous animal studies showed detrimental effects of DBT during in utero development at relatively high doses, but little was known about the effects of DBT exposure at environmentally relevant doses on endpoints such as obesity and metabolic disease. OBJECTIVES: We tested the potential obesogenic effects of DBT using in vitro and in vivo models...
May 2018: Environmental Health Perspectives
https://www.readbyqxmd.com/read/29786867/growth-hormone-releasing-hormone-antagonists-reduce-prostatic-enlargement-and-inflammation-in-carrageenan-induced-chronic-prostatitis
#18
Petra Popovics, Renzhi Cai, Wei Sha, Ferenc G Rick, Andrew V Schally
BACKGROUND: Inflammation plays a key role in the etiology of benign prostatic hyperplasia (BPH) through multiple pathways involving the stimulation of proliferation by cytokines and growth factors as well as the induction of the focal occurrence of epithelial-to-mesenchymal transition (EMT). We have previously reported that GHRH acts as a prostatic growth factor in experimental BPH and in autoimmune prostatitis models and its blockade with GHRH antagonists offer therapeutic approaches for these conditions...
May 21, 2018: Prostate
https://www.readbyqxmd.com/read/29786795/protocols-for-studies-on-stromal-cells-in-prostate-cancer
#19
Damien A Leach, Grant Buchanan
Interactions between tumor cells and fibroblasts play a pivotal role in cancer development and progression. Indeed, the paracrine communication between these two cell types is known to have physiological effects that alter carcinogenic and metastatic potential. An often overlooked player in these interactions is the involvement of the extracellular matrix (ECM). The network of ECM proteins secreted from fibroblasts is reportedly altered with cancer initiation and progression, and in several cases has been associated with patient outcome...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29786517/estrogen-receptor-%C3%AE-potential-target-for-therapy-in-adult-granulosa-cell-tumors
#20
Alessandra Ciucci, Gabriella Ferrandina, Floriana Mascilini, Flavia Filippetti, Giovanni Scambia, Gian Franco Zannoni, Daniela Gallo
OBJECTIVE: Adult granulosa cell tumor (AGCT) is a rare form of sex-cord stromal ovarian tumors. Due to their origin, AGCTs secrete estrogens, and thus, estrogen receptor (ER)-mediated signaling has been considered as a possible target for therapy. The aim of the present study was to get insights into estrogen receptor status and activity in AGCTs, as a strategy to provide molecular support for personalized hormonal treatments. METHODS: We evaluated by immunohistochemistry the expression of ERα, ERβ isoforms (i...
May 18, 2018: Gynecologic Oncology
keyword
keyword
1229
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"