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https://www.readbyqxmd.com/read/29031226/ectopic-vascularized-bone-formation-by-human-mesenchymal-stem-cell-microtissues-in-a-biocomposite-scaffold
#1
Rajan Narayan, Tarun Agarwal, Debasish Mishra, Somnath Maji, Sujata Mohanty, Asok Mukhopadhyay, Tapas Kumar Maiti
Three-dimensional multicellular human bone marrow mesenchymal stem cells (hBM-MSCs) are showing a great promise in the repair of bone tissue due to its osteogenic differentiation potential, mimicking in vivo microenvironment and immunomodulatory property. In the present study, the potential of hBM-MSC microtissues (MTs) in combination with a biocomposite material to form vascularized bone-like tissue at an ectopic site in an immunocompromised mouse was evaluated. The scaffold was fabricated using gelatin, carboxymethyl cellulose, polyvinyl alcohol and nano-hydroxyapatite (GCnHP) by the freeze-drying method...
October 5, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29031176/a-case-of-dedifferentiated-liposarcoma-of-the-heart-and-stomach
#2
Yoichi Hisata, Yuichi Tasaki, Satoshi Kozaki, Takafumi Yamada
INTRODUCTION: Liposarcoma of the heart and stomach is rare. PRESENTAION OF CASE: We report a case of liposarcoma in both organs with dedifferentiated histology. A patient was referred to our hospital with anorexia and weight loss. Upper gastrointestinal tract endoscopy revealed 5-10-mm elevated lesions, and echocardiography and computed tomography showed tumorous lesions in the left atrium. Tumor resection and mitral valve replacement were performed, and biopsy was performed for the gastric tumor...
October 6, 2017: International Journal of Surgery Case Reports
https://www.readbyqxmd.com/read/29029361/the-mechanism-of-attenuation-of-epithelial-mesenchymal-transition-by-a-phosphodiesterase-5-inhibitor-via-renal-klotho-expression
#3
Seung Tae Han, Jae Seok Kim, Jun Young Lee, Min Keun Kim, Jin Sae Yoo, Byoung Geun Han, Seung Ok Choi, Jae Won Yang
OBJECTIVES: Phosphodiesterase-5 (PDE-5) inhibitors induces vasodilation in several organs by blocking cyclic GMP (guanosine monophosphate) degradation. However, the existence of alternative mechanism of action in case of an impaired nitric oxide (NO) system remains controversial. Previous studies suggested that decreased NO bioavailability may result in the down-regulation of klotho expression, but the relationship between klotho and NO remains obscure. Therefore, we investigated whether a PDE-5 inhibitor could preserve epithelial mesenchymal transition (EMT) and relationship exists between the NO and renal klotho expression...
October 13, 2017: Clinical and Experimental Pharmacology & Physiology
https://www.readbyqxmd.com/read/29028795/alx4-relays-sequential-fgf-signaling-to-induce-lacrimal-gland-morphogenesis
#4
Ankur Garg, Mukesh Bansal, Noriko Gotoh, Gen-Sheng Feng, Jian Zhong, Fen Wang, Ariana Kariminejad, Steven Brooks, Xin Zhang
The sequential use of signaling pathways is essential for the guidance of pluripotent progenitors into diverse cell fates. Here, we show that Shp2 exclusively mediates FGF but not PDGF signaling in the neural crest to control lacrimal gland development. In addition to preventing p53-independent apoptosis and promoting the migration of Sox10-expressing neural crests, Shp2 is also required for expression of the homeodomain transcription factor Alx4, which directly controls Fgf10 expression in the periocular mesenchyme that is necessary for lacrimal gland induction...
October 13, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/29028628/role-of-pdgf-bb-in-proliferation-differentiation-and-maintaining-stem-cell-properties-of-pdl-cells-in-vitro
#5
Zornitsa Mihaylova, Rozaliya Tsikandelova, Pavel Sanimirov, Natalia Gateva, Vanyo Mitev, Nikolay Ishkitiev
OBJECTIVE: Platelet-derived growth factor-BB (PDGF-BB) is one of the most abundant growth factors in platelet derived products and has been shown to stimulate regeneration after tissue injury. There is a population of mesenchymal stem cells (MSC) in human periodontal ligament (PDL) which can contribute to tissue regeneration under appropriate conditions. DESIGN: PDL cells were isolated and characterized using stem cell and differentiation markers via immunofluorescence and flow cytometry and then cultured in vitro and treated with different concentrations of PDGF-BB...
September 25, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/29028086/downregulation-of-microrna-645-suppresses-breast-cancer-cell-metastasis-via-targeting-dcdc2
#6
Y Cai, W-F Li, Y Sun, K Liu
OBJECTIVE: To analyze the functioning mode of miR-645 on breast cancer cell metastasis and provide therapeutic targets for breast cancer. MATERIALS AND METHODS: Quantitative Real-time PCR (qRT-PCR) assay was employed to detect miR-645 expression level. Wound healing assay and transwell assay were performed to investigate metastasis capacity of breast cancer cells. Protein levels were assessed by Western blotting assay. The target gene was predicted and verified by bioinformatics analysis and luciferase assay...
September 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29028071/how-to-isolate-a-ready-to-use-adipose-derived-stem-cells-pellet-for-clinical-application
#7
E Raposio, N Bertozzi
Adipose-derived stem cells (ASCs) are multipotent mesenchymal stem cells (MSCs) that show definitive stem cell characteristics such as plastic adherence in culture, ability to maintain multipotency upon in vitro expansion, and self-renewal capacity. ASCs are particularly promising for use in regenerative medicine because they can be harvested easily from adipose tissue by standard liposuction, with minimal donor site morbidity. Since ASCs do not necessitate ex vivo expansion to obtain clinically significant cell numbers, it is critical to identify a standardized method that maximizes the number of ASCs collected...
September 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29027958/adipose-tissue-as-a-strategic-source-of-mesenchymal-stem-cells-in-bone-regeneration-a-topical-review-on-the-most-promising-craniomaxillofacial-applications
#8
REVIEW
Francesco Paduano, Massimo Marrelli, Massimiliano Amantea, Carlo Rengo, Sandro Rengo, Michel Goldberg, Gianrico Spagnuolo, Marco Tatullo
Bone regeneration in craniomaxillofacial surgery represents an issue that involves both surgical and aesthetic aspects. The most recent studies on bone tissue engineering involving adipose-derived stromal/stem cells (ASCs) have clearly demonstrated that such cells can play a crucial role in the treatment of craniomaxillofacial defects, given their strong commitment towards the osteogenic phenotype. A deeper knowledge of the molecular mechanisms underlying ASCs is crucial for a correct understanding of the potentialities of ASCs-based therapies in the most complex maxillofacial applications...
October 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29027917/porcine-dental-epithelial-cells-differentiated-in-a-cell-sheet-constructed-by-magnetic-nanotechnology
#9
Wataru Koto, Yoshinori Shinohara, Kazuyuki Kitamura, Takanori Wachi, Seicho Makihira, Kiyoshi Koyano
Magnetic nanoparticles (MNPs) are widely used in medical examinations, treatments, and basic research, including magnetic resonance imaging, drug delivery systems, and tissue engineering. In this study, MNPs with magnetic force were applied to tissue engineering for dental enamel regeneration. The internalization of MNPs into the odontogenic cells was observed by transmission electron microscopy. A combined cell sheet consisting of dental epithelial cells (DECs) and dental mesenchymal cells (DMCs) (CC sheet) was constructed using magnetic force-based tissue engineering technology...
October 13, 2017: Nanomaterials
https://www.readbyqxmd.com/read/29027713/engineering-cell-surface-function-with-dna-origami
#10
Ehsan Akbari, Molly Y Mollica, Christopher R Lucas, Sarah M Bushman, Randy A Patton, Melika Shahhosseini, Jonathan W Song, Carlos E Castro
A specific and reversible method is reported to engineer cell-membrane function by embedding DNA-origami nanodevices onto the cell surface. Robust membrane functionalization across epithelial, mesenchymal, and nonadherent immune cells is achieved with DNA nanoplatforms that enable functions including the construction of higher-order DNA assemblies at the cell surface and programed cell-cell adhesion between homotypic and heterotypic cells via sequence-specific DNA hybridization. It is anticipated that integration of DNA-origami nanodevices can transform the cell membrane into an engineered material that can mimic, manipulate, and measure biophysical and biochemical function within the plasma membrane of living cells...
October 13, 2017: Advanced Materials
https://www.readbyqxmd.com/read/29027711/expansion-of-mesenchymal-stem-cells-on-electrospun-scaffolds-maintains-stemness-mechano-responsivity-and-differentiation-potential
#11
Su-Jin Heo, Spencer E Szczesny, Dong Hwa Kim, Kamiel S Saleh, Robert L Mauck
Mesenchymal stem cells (MSCs) hold great promise for regenerative therapies and tissue engineering applications given their multipotential differentiation capacity. However, MSC isolation and expansion are typically performed on super-physiologically stiff tissue culture plastic (TCP), which may alter their behavior and lead to unintended consequences upon implantation. In contrast, electrospun nanofibrous scaffolds possess physical and mechanical properties that are similar to that of native tissue. In this study, we investigated whether isolation and expansion of juvenile bovine MSCs directly onto electrospun nanofibrous scaffolds better preserves MSC phenotype and stemness compared to TCP...
October 13, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/29027626/emerging-roles-for-lpp-in-metastatic-cancer-progression
#12
REVIEW
Elaine Ngan, Alex Kiepas, Claire M Brown, Peter M Siegel
LIM domain containing proteins are important regulators of diverse cellular processes, and play pivotal roles in regulating the actin cytoskeleton. Lipoma Preferred Partner (LPP) is a member of the zyxin family of LIM proteins that has long been characterized as a promoter of mesenchymal/fibroblast cell migration. More recently, LPP has emerged as a critical inducer of tumor cell migration, invasion and metastasis. LPP is thought to contribute to these malignant phenotypes by virtue of its ability to shuttle into the nucleus, localize to adhesions and, most recently, to promote invadopodia formation...
October 13, 2017: Journal of Cell Communication and Signaling
https://www.readbyqxmd.com/read/29027570/are-we-right-to-consider-mesenchymal-stem-cells-to-be-a-new-perspective-for-patients-with-juvenile-idiopathic-arthritis
#13
REVIEW
Krzysztof Orczyk, Elzbieta Smolewska
Juvenile idiopathic arthritis (JIA) is the most common cause of chronic arthritis in childhood. Up to 50% of patients are resistant to standard therapy, which includes non-steroid anti-inflammatory drugs, corticosteroids, disease-modifying anti-rheumatic drugs and biologic therapies. Intra-articular injection of mesenchymal stem cells (MSCs) is proposed as a new approach to JIA treatment. MSCs can modulate inflammation via mechanisms of both adaptive and innate immune response. They are able to inhibit T and B cell proliferation, promote regulatory T cells, suppress the maturation of dendritic cells, stimulate macrophage differentiation into M2 phenotype and reduce effectiveness of natural killer cells...
October 13, 2017: Archivum Immunologiae et Therapiae Experimentalis
https://www.readbyqxmd.com/read/29027529/-twin-pregnancy-with-complete-hydatidiform-mole
#14
I N Voloshchuk, I V Barinova, N I Kondrikov, R A Barto
The paper describes a case of twin pregnancy with complete hydatidiform mole (CHM). According to the data available in the literature, the concurrence of CHM with a normal placenta and a viable fetus occurs in 1 per 20,000-100,000 pregnancies, requires a differential diagnosis with partial hydatidiform mole and placental mesenchymal dysplasia, and is characterized by a high rate of complications. In this concurrence, the frequency of persistent trophoblastic disease is as high as 50%. In this case, the pregnancy ended in a spontaneous abortion at 16-17 weeks of pregnancy...
2017: Arkhiv Patologii
https://www.readbyqxmd.com/read/29027451/-enhancing-the-ability-of-autophagy-and-proliferation-of-bone-marrow-mesenchymal-stem-cells-by-interleukin-8-through-akt-stat3-pathway-in-hypoxic-environment
#15
Lei Shen, Shanqiang Zhang, Xiaodong Zhang, Yuting Zhang, Liping Xie, Yang Jiang, Yong Ma, Guofeng Li
To study the effects and mechanisms of interleukin-8 (IL-8) on the proliferation and autophagy of human bone marrow mesenchymal stem cells (hBMSC) under hypoxic condition. In the hypoxia model, we set the non-stimulated hBMSC as the hypoxia control group; the hBMSC stimulated by 100 μmol/L human IL-8 as the IL-8 group; the hBMSC stimulated by 50 μmol/L MK2206 (Akt protein inhibitor) and 100μmol/L IL-8 as the Akt inhibitor group; and the normal cultured hBMSC as the normal control group. The experiments of EdU cell proliferation and TUNEL apoptosis were respectively used to detect the number of positive cells that were labeled by EdU and apoptosis in each group, and Western blotting and ELISA were used respectively to detect the expression of autophagy protein (LC-3), Akt/STAT3 and other proteins in each group...
October 25, 2016: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/29027282/oestrogen-retains-human-periodontal-ligament-stem-cells-stemness-in-long-term-culture
#16
Qianmin Ou, Xiaoxiao Wang, Yanlan Wang, Yan Wang, Xuefeng Lin
OBJECTIVES: During long-term culture, loss of stemness is observed which greatly restricts the application of human periodontal ligament stem cells (hPDLSCs) in tissue regeneration. Oestrogen (E2) was found to significantly enhance the proliferation and osteogenic differentiation capacity in mesenchymal stem cells. Therefore, in this study, we investigated effects of E2 on hPDLSCs stemness in long-term culture. MATERIALS AND METHODS: Effects of E2 on hPDLSCs stemness were systematically evaluated...
October 12, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/29027279/long-non-coding-rna-pvt1-emerging-biomarker-in-digestive-system-cancer
#17
REVIEW
Dan-Dan Zhou, Xiu-Fen Liu, Cheng-Wei Lu, Om Prakash Pant, Xiao-Dong Liu
The digestive system cancers are leading cause of cancer-related death worldwide, and have high risks of morbidity and mortality. More and more long non-coding RNAs (lncRNAs) have been studied to be abnormally expressed in cancers and play a key role in the process of digestive system tumour progression. Plasmacytoma variant translocation 1 (PVT1) seems fairly novel. Since 1984, PVT1 was identified to be an activator of MYC in mice. Its role in human tumour initiation and progression has long been a subject of interest...
October 12, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/29027191/tha-conversion-rate-comparing-decompression-alone-with-autologous-bone-graft-or-stem-cells-in-osteonecrosis
#18
Francisco J Nally, Gerardo Zanotti, Martín A Buttaro, Fernando D Dilernia, Ignacio G Mansilla, Fernando M Comba, Francisco Piccaluga
INTRODUCTION: Core decompression (CD) with or without the addition of autologous bone graft (CDBG) is currently the most widely accepted treatment for hip osteonecrosis in stages of pre-collapse. The use of bone marrow mesenchymal stem cells (CDSC) is emerging as a promising biological alternative. We sought to determine the total hip arthroplasty (THA) conversion rate for patients treated with CD, CDBG or CDSC. MATERIAL AND METHODS: Between 1996 and 2012, 97 cases were evaluated in 72 patients: 47 CD cases, 34 CDGB cases and 16 CDSC cases...
October 9, 2017: Hip International: the Journal of Clinical and Experimental Research on Hip Pathology and Therapy
https://www.readbyqxmd.com/read/29027140/biosafety-and-antibacterial-ability-of-graphene-and-graphene-oxide-in-vitro-and-in-vivo
#19
Long Pang, Chunqiu Dai, Long Bi, Zhongshang Guo, Junjun Fan
In recent years, graphene (G) and graphene oxide (GO) nanoparticles have begun to be applied in surgical implant surface modification. However, biosafety and antibacterial ability of G and GO are still unclear. In this study, the biosafety of G and GO in vitro was evaluated by co-culture with bone marrow mesenchymal stem cells (BMSCs) and biosafety in vivo was observed by implanting materials into mice muscle tissue. Biosafety results showed that 10 μg/ml was the safety critical concentration for G and GO...
October 12, 2017: Nanoscale Research Letters
https://www.readbyqxmd.com/read/29027119/mollugin-enhances-the-osteogenic-action-of-bmp-2-via-the-p38-smad-signaling-pathway
#20
Seong-Hee Moon, Ikyon Kim, Seong Hwan Kim
Bone morphogenetic protein 2 (BMP-2) has been used clinically to encourage bone regeneration; although, there can be major side effects with larger doses. Therefore, there is a need to identify new small molecules to potentiate the osteogenic action of BMP-2. In this study, we investigated the effect of mollugin on bone formation in murine bi-potential mesenchymal progenitor C2C12 cells by combination with BMP-2. We found mollugin could enhance the BMP-2-mediated osteoblast differentiation of C2C12 cells. This was accompanied by the induction of other osteogenic BMPs...
October 12, 2017: Archives of Pharmacal Research
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