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Monoclonal antibodies

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https://www.readbyqxmd.com/read/29677631/enhancement-of-monoclonal-antibody-production-after-single-and-combination-treatment-of-the-hybridoma-cells-with-all-trans-retinoic-acid-and-docosahexaenoic-acid-an-in-vitro-and-in-vivo-study
#1
Mohsen Rokni, Ali Reza Razavi, Fazel Shokri, Kazem Ahmadi Kia, Farid Solaymani-Mohammadi, Reza Chahardoli, Ali Akbar Saboor-Yaraghi
Murine hybridoma cells can produce monoclonal antibody (MAb) and the production of these antibodies in culture and peritoneum can be affected by different factors, including stimulants, inhibitors and supplements. Among these factors, the impact of micronutrients on the production of MAbs by mouse hybridoma cells has not fully been explored. In this study the murine hybridoma cells, M3C5, were cultured and treated with different concentrations of ATRA and DHA, alone, in combinations, and at different time of exposure...
April 17, 2018: International Immunopharmacology
https://www.readbyqxmd.com/read/29677301/association-between-baseline-ldl-c-level-and-total-and-cardiovascular-mortality-after-ldl-c-lowering-a-systematic-review-and-meta-analysis
#2
Eliano P Navarese, Jennifer G Robinson, Mariusz Kowalewski, Michalina Kolodziejczak, Felicita Andreotti, Kevin Bliden, Udaya Tantry, Jacek Kubica, Paolo Raggi, Paul A Gurbel
Importance: Effects on specific fatal and nonfatal end points appear to vary for low-density lipoprotein cholesterol (LDL-C)-lowering drug trials. Objective: To evaluate whether baseline LDL-C level is associated with total and cardiovascular mortality risk reductions. Data Sourcesand Study Selection: Electronic databases (Cochrane, MEDLINE, EMBASE, TCTMD, ClinicalTrials.gov, major congress proceedings) were searched through February 2, 2018, to identify randomized clinical trials of statins, ezetimibe, and PCSK9-inhibiting monoclonal antibodies...
April 17, 2018: JAMA: the Journal of the American Medical Association
https://www.readbyqxmd.com/read/29677240/uveal-effusion-after-immune-checkpoint-inhibitor-therapy
#3
Merina Thomas, Stephen T Armenti, M Bernadete Ayres, Hakan Demirci
Importance: Immune checkpoint inhibitors, including antiprogrammed cell death protein-1 (anti-PD-1) and antiprogrammed cell death ligand-1 (anti-PD-L1) monoclonal antibodies, have recently been introduced as a promising new immunotherapy for solid cancers. The adverse effects typically include inflammation of the skin, endocrine, and gastrointestinal systems. Objective: To describe 3 patients who developed uveal effusion after initiating anti-PD-1 and anti-PD-L1 monoclonal antibody therapy...
April 12, 2018: JAMA Ophthalmology
https://www.readbyqxmd.com/read/29677174/the-role-of-autophagy-and-the-chemokine-c-x-c-motif-ligand-16-during-acute-lung-injury-in-mice
#4
Ye Gao, Ni Wang, Rui H Li, Yang Z Xiao
BACKGROUND Acute lung injury (ALI) is responsible for mortality in hospitalized patients. Autophagy can negatively regulate inflammatory response, and CXCL16 (chemokine (C-X-C motif) ligand 16) is a kind of chemokine, which is closely related to the inflammatory response. However, the relationship between autophagy and CXCL16 in ALI is still unclear. This study aimed to investigate the role of autophagy and chemokine CXCL16 in ALI in mice. MATERIAL AND METHODS Thirty-two male C57BL/6 mice were divided into four groups...
April 20, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29676864/bioequivalence-of-canakinumab-injected-subcutaneously-via-an-autoinjector-device-or-a-prefilled-safety-syringe-device-in-healthy-subjects
#5
SivaNaga S Anumolu, Sam Lindgren, Janardhana Vemula, David Floch, Christine Reynolds, Hans-Joachim Wallny, Haiying Sun
Canakinumab, a high-affinity human anti-interleukin-1β monoclonal antibody, is being used for the treatment of a broad spectrum of inflammatory diseases. This phase 1 study compared the relative bioavailability of a single dose of subcutaneous canakinumab either self-administered with an autoinjector (AI) or administered by a health care professional (HCP) with a prefilled safety syringe (SS) in healthy subjects. The study enrolled 80 subjects randomized 1:1 to receive 150 mg/mL of a liquid formulation of canakinumab via an AI or SS into either the thigh or abdomen...
April 20, 2018: Clinical Pharmacology in Drug Development
https://www.readbyqxmd.com/read/29676602/tocilizumab-for-the-treatment-of-giant-cell-arteritis
#6
Michael Schirmer, Francesco Muratore, Carlo Salvarani
Giant cell arteritis (GCA) is the most frequent type of vasculitis, occurring in people older than 50 years. So far, treatment has been limited to corticosteroids and methotrexate only. Areas covered: Interleukin-6 (IL-6) plays a role in the pathophysiology of GCA. This review covers recent advances in the treatment of GCA with tocilizumab (TCZ), which specifically binds to both soluble and membrane-bound IL-6R and inhibits IL-6R-mediated signaling. Expert commentary: Two randomized controlled trials recently showed the efficacy of the IL-6 receptors inhibitor monoclonal antibody TCZ for the induction and maintenance of remission in patients with new-onset and relapsing GCA...
April 20, 2018: Expert Review of Clinical Immunology
https://www.readbyqxmd.com/read/29676460/resistance-to-proteasome-inhibitors-and-other-targeted-therapies-in-myeloma
#7
REVIEW
Craig T Wallington-Beddoe, Magdalena Sobieraj-Teague, Bryone J Kuss, Stuart M Pitson
The number of novel therapies for the treatment of myeloma is rapidly increasing, as are the clinical trials evaluating them in combination with other novel and established therapies. Proteasome inhibitors, immunomodulatory agents and monoclonal antibodies are the most well known and studied classes of novel agents targeting myeloma, with histone deacetylase inhibitors, nuclear export inhibitors and several other approaches also being actively investigated. However, in parallel with the development and clinical use of these novel myeloma therapies is the emergence of novel mechanisms of resistance, many of which remain elusive, particularly for more recently developed agents...
April 20, 2018: British Journal of Haematology
https://www.readbyqxmd.com/read/29676212/pro-140-a-monoclonal-antibody-targeting-ccr5-as-a-long-acting-single-agent-maintenance-therapy-for-hiv-1-infection
#8
Kush Dhody, Nader Pourhassan, Kazem Kazempour, Derry Green, Shide Badri, Hana Mekonnen, Paul J Maddon, Denis Burger
Background PRO 140 is a humanized monoclonal antibody targeting CCR5 with potent antiviral activity in patients with CCR5-tropic HIV-1 infection. In phase 2b studies, we evaluated the long-term efficacy, safety, and tolerability of PRO 140 monotherapy in maintaining viral suppression for over 24 months in patients who were stable on combination antiretroviral therapy on entry into the trials. Methods and Results Forty-one adult patients, infected exclusively with CCR5-tropic HIV-1 with viral loads <50 copies/mL, were switched from daily oral combination ART regimens to weekly PRO 140 monotherapy for 12 weeks...
April 20, 2018: HIV Clinical Trials
https://www.readbyqxmd.com/read/29675744/ibalizumab-first-global-approval
#9
Anthony Markham
TaiMed Biologics is developing ibalizumab (Trogarzo™, ibalizumab-uiyk)-a humanised IgG4 monoclonal antibody-as a treatment for HIV-1 infection. Ibalizumab blocks HIV entry into CD4 cells while preserving normal immunological function and is the first CD4-directed post-attachment HIV-1 inhibitor and the first humanised monoclonal antibody for the treatment of HIV/AIDS. This article summarizes the milestones in the development of ibalizumab leading to this first approval in HIV-1 treatment.
April 19, 2018: Drugs
https://www.readbyqxmd.com/read/29674997/development-by-genetic-immunization-of-monovalent-antibodies-against-human-vasoactive-intestinal-peptide-receptor-1-vpac1-new-innovative-and-versatile-tools-to-study-vpac1-receptor-function
#10
Xavier Peyrassol, Toon Laeremans, Vannessa Lahura, Maja Debulpaep, Hassan El Hassan, Jan Steyaert, Marc Parmentier, Ingrid Langer
Multi-membrane spanning proteins, such as G protein-coupled receptors (GPCRs) and ion channels, are extremely difficult to purify as native proteins. Consequently, the generation of antibodies that recognize the native conformation can be challenging. By combining genetic immunization, phage display, and biopanning, we identified a panel of monovalent antibodies (nanobodies) targeting the vasoactive intestinal peptide receptor 1 (VPAC1) receptor. The nine unique nanobodies that were classified into four different families based on their CDR3 amino acid sequence and length, were highly specific for the human receptor and bind VPAC1 with moderate affinity...
2018: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/29674708/population-structure-of-environmental-and-clinical-legionella-pneumophila-isolates-in-catalonia
#11
Sara Quero, Noemí Párraga-Niño, Irene Barrabeig, Maria Rosa Sala, M Luisa Pedro-Botet, Eduard Monsó, Mireia Jané, Miquel Sabrià, Marian Garcia-Núñez
Legionella is the causative agent of Legionnaires' disease (LD). In Spain, Catalonia is the region with the highest incidence of LD cases. The characterisation of clinical and environmental isolates using molecular epidemiology techniques provides epidemiological data for a specific geographic region and makes it possible to carry out phylogenetic and population-based analyses. The aim of this study was to describe and compare environmental and clinical isolates of Legionella pneumophila in Catalonia using sequence-based typing and monoclonal antibody subgrouping...
April 19, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29674421/anti-misrii-radiolabeled-antibodies-new-tools-for-a-theranostic-approach-in-ovarian-cancer
#12
Emmanuel Deshayes, Riad Ladjohounlou, Pierre Le Fur, Alexandre Pichard, Catherine Lozza, Vincent Boudousq, Samuel Sevestre, Marta Jarlier, Roxana Kashani, Joanna Koch, Jane Sosabowski, Julie Foster, Nicolas Chouin, Frank Bruchertseifer, Alfred Morgenstern, Pierre-Olivier Kotzki, Isabelle Navarro-Teulon, Jean-Pierre Pouget
We have developed the 16F12 mouse monoclonal antibody (mAb) that targets the MISRII receptor expressed by ovarian tumors. Here, we assessed in preclinical models the possibility to use radiolabeled 16F12 in a theranostic approach for small-volume ovarian peritoneal carcinomatosis, for instance after cytoreductive surgery. Methods: DOTA-, DTPA- or DFO-conjugated 16F12 mAb was radiolabeled with beta (177 Lu) or alpha (213 Bi) particle emitters for therapeutic use, and with 89 Zr for positron emission tomography (PET) imaging...
April 19, 2018: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/29674076/a-facile-route-to-glycated-albumin-detection
#13
Nadra Bohli, Olivier Meilhac, Philippe Rondeau, Syrine Gueffrache, Laurence Mora, Adnane Abdelghani
In this paper we propose an easy way to detect the glycated form of human serum albumin which is biomarker for several diseases such as diabetes and Alzheimer. The detection platform is a label free impedimetric immunosensor, in which we used a monoclonal human serum albumin antibody as a bioreceptor and electrochemical impedance as a transducing method. The antibody was deposited onto a gold surface by simple physisorption technique. Bovine serum albumin was used as a blocking agent for non-specific binding interactions...
July 1, 2018: Talanta
https://www.readbyqxmd.com/read/29674074/fluorescent-microarray-for-multiplexed-quantification-of-environmental-contaminants-in-seawater-samples
#14
Ana Sanchis, J-Pablo Salvador, Katrina Campbell, Christopher T Elliott, Weilin L Shelver, Qing X Li, M-Pilar Marco
The development of a fluorescent multiplexed microarray platform able to detect and quantify a wide variety of pollutants in seawater is reported. The microarray platform has been manufactured by spotting 6 different bioconjugate competitors and it uses a cocktail of 6 monoclonal or polyclonal antibodies raised against important families of chemical pollutants such as triazine biocide (i.e. Irgarol 1051®), sulfonamide and chloramphenicol antibiotics, polybrominated diphenyl ether flame-retardant (PBDE, i.e...
July 1, 2018: Talanta
https://www.readbyqxmd.com/read/29674041/optimization-of-classification-and-regression-analysis-of-four-monoclonal-antibodies-from-raman-spectra-using-collaborative-machine-learning-approach
#15
Laetitia Minh Maï Le, Balázs Kégl, Alexandre Gramfort, Camille Marini, David Nguyen, Mehdi Cherti, Sana Tfaili, Ali Tfayli, Arlette Baillet-Guffroy, Patrice Prognon, Pierre Chaminade, Eric Caudron
The use of monoclonal antibodies (mAbs) constitutes one of the most important strategies to treat patients suffering from cancers such as hematological malignancies and solid tumors. These antibodies are prescribed by the physician and prepared by hospital pharmacists. An analytical control enables the quality of the preparations to be ensured. The aim of this study was to explore the development of a rapid analytical method for quality control. The method used four mAbs (Infliximab, Bevacizumab, Rituximab and Ramucirumab) at various concentrations and was based on recording Raman data and coupling them to a traditional chemometric and machine learning approach for data analysis...
July 1, 2018: Talanta
https://www.readbyqxmd.com/read/29673975/tlr-2-il-10-and-il-17-mediated-immunity-in-experimental-chemotherapy-murine-model-of-systemic-candidiasis-cyclophosphamides-impact-and-roles
#16
Parvin Dehghan, Sepideh Tolouie, Behzad Baradaran, Sanam Nami, Hamid Morovati
The majority of immune components such as Toll-like receptor (TLR)-2, interleukin (IL)-17, neutrophils, and IL-10 play pivotal roles in immunity to Candida albicans (C. albicans) through identifying and launching inflammatory and regulatory responses. Chemotherapy is one of the most potent risk factors for systemic candidiasis through inducing immunosuppression (mostly cyclophosphamide induced immunosuppression) and there is a sensible lack of study around the immunity to C. albicans in such a situation. In this study, following the establishment of infection and immunosuppression in Balb/c mice model, the mRNA/protein levels of TLR-2, IL-10, IL-17, and Myeloperoxidase (MPO) in serum/kidney were measured using Real-time PCR and ELISA respectively...
April 16, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29673969/rgd-cadherins-and-%C3%AE-2%C3%AE-1-integrin-in-cancer-metastasis-a-dangerous-liaison
#17
REVIEW
J Ignacio Casal, Rubén A Bartolomé
We propose a new cadherin family classification comprising epithelial cadherins (cadherin 17 [CDH17], cadherin 16, VE-cadherin, cadherin 6 and cadherin 20) containing RGD motifs within their sequences. Expression of some RGD cadherins is associated with aggressive forms of cancer during the late stages of metastasis, and CDH17 and VE-cadherin have emerged as critical actors in cancer metastasis. After binding to α2β1 integrin, these cadherins promote integrin β1 activation, and thereby cell adhesion, invasion and proliferation, in liver and lung metastasis...
April 16, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29673745/-acquired-c1-esterase-inhibitor-deficiency-via-bradykinin-mediated-angioedema-four-cases
#18
C Jacquin-Porretaz, F Castelain, E Daguindau, E Seilles, C Nardin, F Aubin, F Pelletier
BACKGROUND: Acquired C1-esterase inhibitor (C1-INH) deficiency angioedema (C1-INH-AAE) is a form of bradykinin-mediated angioedema. This rare disorder is due to acquired consumption of C1-INH, hyperactivation of the classic pathway of human complement, and potentially fatal recurrent angioedema symptoms. Clinical symptoms of C1-INH-AAE are very similar to those of hereditary angioedema (HAE) but usually appear after the fourth decade of life and induce abdominal pain less frequently. Laboratory tests are essential in establishing the diagnosis with low levels or abnormal structure and function of C1-INH...
April 16, 2018: Annales de Dermatologie et de Vénéréologie
https://www.readbyqxmd.com/read/29673644/3d-mesoscopic-fluorescence-tomography-for-imaging-micro-distribution-of-antibody-photon-absorber-conjugates-during-near-infrared-photoimmunotherapy-in-vivo
#19
Qinggong Tang, Tadanobu Nagaya, Yi Liu, Hannah Horng, Jonathan Lin, Kazuhide Sato, Hisataka Kobayashi, Yu Chen
As a novel low-side-effect cancer therapy, photo-immunotherapy (PIT) is based on conjugating monoclonal antibody (mAb) with a near-infrared (NIR) phthalocyanine dye IRDye700DX (IR 700). IR700 is not only fluorescent to be used as an imaging agent, but also phototoxic. When illuminating with NIR light, PIT can induce highly-selective cancer cell death while leaving most of tumor blood vessels unharmed, leading to an effect termed super-enhanced permeability and retention (SUPR), which can significantly improve the effectiveness of anti-cancer drug...
April 16, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29673459/-preparation-characterization-and-application-of-monoclonal-antibody-against-recq-helicase
#20
Ronghong Zhang, Tiewen Jia, Fang Lian, Jielin Liu, Meng Zhou
Objective To prepare monoclonal antibodies (mAb) against RecQ helicase, characterize their biological properties, and then investigate their activity against RecQ helicase in tumor cells. Methods BALB/c mice were immunized with purified recombinant RecQ helicase from E.coli. Hybridoma technique was used to generate the mAb against RecQ helicase. The chromosome karyotype analysis of the hybridoma cells was performed by colchicine blocking. The Ig subtype and titer of mAb in ascitic fluid were determined by ELISA...
February 2018: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
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