Rafael F Castelli, Alana Pereira, Leandro Honorato, Alessandro Valdez, Haroldo C de Oliveira, Jaqueline M Bazioli, Ane W A Garcia, Tabata D'Maiella Freitas Klimeck, Flavia C G Reis, Amanda C Camillo-Andrade, Marlon D M Santos, Paulo C Carvalho, Oscar Zaragoza, Charley C Staats, Leonardo Nimrichter, Taícia P Fill, Marcio L Rodrigues
Small molecules are components of fungal extracellular vesicles (EVs), but their biological roles are only superficially known. NOP16 is a eukaryotic gene that is required for the activity of benzimidazoles against Cryptococcus deuterogattii . In this study, during the phenotypic characterization of C. deuterogattii mutants expected to lack NOP16 expression, we observed a reduced EV production. Whole-genome sequencing, RNA-Seq, and cellular proteomics revealed that, contrary to our initial findings, these mutants expressed Nop16 but exhibited altered expression of 14 genes potentially involved in sugar transport...
March 12, 2024: Infection and Immunity