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https://www.readbyqxmd.com/read/29334709/-molecular-epidemiological-study-of-human-coronavirus-oc43-in-shanghai-from-2009-2016
#1
Y J Yang, Y W Hu
Objective: To understand the epidemiological characteristics of Human coronavirus (HCoV), the patterns of emergence and circulation, and the genotype distribution of human coronavirus OC43 (HCoV-OC43) from November, 2009 to April, 2016 in Shanghai. Methods: A total of 6 059 respiratory specimens, including pharyngeal swab, sputum, nasopharyngeal aspirates and alveolar lavage fluid, as well as relative clinical data were collected from patients with acute respiratory infections from seven sentinel hospitals during November, 2009 to April, 2016 in Shanghai...
January 6, 2018: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
https://www.readbyqxmd.com/read/29333771/-effect-of-hydrofluoric-acid-concentration-on-the-surface-morphology-and-bonding-effectiveness-of-lithium-disilicate-glass-ceramics-to-resin-composites
#2
Qian Hailan, Ren Lingyan, Nie Rongrong, Meng Xiangfeng
OBJECTIVE: This study aimed at determining the influence of hydrofluoric acid (HF) in varied concentrations on the surface morphology of lithium disilicate glass ceramics and bond durability between resin composites and post-treated lithium disilicate glass ceramics. METHODS: After being sintered, ground, and washed, 72 as-prepared specimens of lithium disilicate glass ceramics with dimensions of 11 mm×13 mm×2 mm were randomly divided into three groups. Each group was treated with acid solution [32% phosphoric acid (PA) or 4% or 9...
December 1, 2017: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/29332472/statistical-primer-for-athletic-trainers-the-essentials-of-understanding-measures-of-reliability-and-minimal-important-change
#3
Bryan L Riemann, Monica Lininger
OBJECTIVE:   To describe the concepts of measurement reliability and minimal important change. BACKGROUND:   All measurements have some magnitude of error. Because clinical practice involves measurement, clinicians need to understand measurement reliability. The reliability of an instrument is integral in determining if a change in patient status is meaningful. DESCRIPTION:   Measurement reliability is the extent to which a test result is consistent and free of error...
January 13, 2018: Journal of Athletic Training
https://www.readbyqxmd.com/read/29331874/thyroid-hormone-resuscitation-after-brain-death-in-potential-organ-donors-a-primer-for-neurocritical-care-providers-and-narrative-review-of-the-literature
#4
REVIEW
Ian A Buchanan, Vivek A Mehta
Solid organ transplantation has become a mainstay in the contemporary management of end-stage organ failures fueled by advances in immunosuppression, intensive care and surgical technology. Every year, a vast number of transplantable organs is lost on account of hemodynamic instability in potential brain-dead organ donors. Because of a growing organ shortage, measures that increase total donor supply pools are desperately needed. Thyroid hormone has been identified as an adjunctive therapy in donor management due to its potential for increasing organ supply and is currently endorsed by transplant organizations such as United Network for Organ Sharing (UNOS)...
January 8, 2018: Clinical Neurology and Neurosurgery
https://www.readbyqxmd.com/read/29331077/optimized-paired-sgrna-cas9-cloning-and-expression-cassette-triggers-high-efficiency-multiplex-genome-editing-in-kiwifruit
#5
Zupeng Wang, Shuaibin Wang, Dawei Li, Qiong Zhang, Li Li, Caihong Zhong, Yifei Liu, Hongwen Huang
Kiwifruit is an important fruit crop; however, technologies for its functional genomic and molecular improvement are limited. The clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) system has been successfully applied to genetic improvement in many crops, but its editing capability is variable depending on the different combinations of the synthetic guide RNA (sgRNA) and Cas9 protein expression devices. Optimizing conditions for its use within a particular species is therefore needed to achieve highly efficient genome editing...
January 13, 2018: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29329946/a-primer-on-caspase-mechanisms
#6
REVIEW
Monica Gonzalez Ramirez, Guy S Salvesen
Caspases belong to a diverse clan of proteolytic enzymes known as clan CD with highly disparate functions in cell signaling. The caspase members of this clan are only found in animals, and most of them orchestrate the demise of cells by the highly distinct regulated cell death phenotypes known as apoptosis and pyroptosis. This review looks at the mechanistic distinctions between the activity and activation mechanisms of mammalian caspases compared to other members of clan CD. We also compare and contrast the role of different caspase family members that program anti-inflammatory and pro-inflammatory cell death pathways...
January 9, 2018: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/29329935/tick-borne-pathogen-detection-what-s-new
#7
Alejandro Cabezas-Cruz, Muriel Vayssier-Taussat, Gilbert Greub
Ticks and the pathogens they transmit constitute a growing burden for human and animal health worldwide. Traditionally, tick-borne pathogen detection has been carried out using PCR-based methods that rely in known sequences for specific primers design. This approach matches with the view of a 'single-pathogen' epidemiology. Recent results, however, have stressed the importance of coinfections in pathogen ecology and evolution with impact in pathogen transmission and disease severity. New approaches, including high-throughput technologies, were then used to detect multiple pathogens, but they all need a priori information on the pathogens to search...
January 9, 2018: Microbes and Infection
https://www.readbyqxmd.com/read/29329796/the-evolving-face-of-myocardial-reperfusion-in-acute-coronary-syndromes-a-primer-for-the-internist
#8
REVIEW
R Jay Widmer, Peter M Pollak, Malcolm R Bell, Bernard J Gersh, Nandan S Anavekar
Acute coronary syndromes (ACSs) account for a large proportion of disease burden in the United States and worldwide, and our understanding of ACS management continues to evolve. In this review we take a practical approach to evaluating and treating a patient with ACS, focusing on the optimal timing and methods of coronary reperfusion. Beginning with initial assessment and risk stratification, a provider managing the patient with ACS must be able to expeditiously decide on and implement the correct guideline-directed pathway to optimize outcomes...
January 9, 2018: Mayo Clinic Proceedings
https://www.readbyqxmd.com/read/29329457/a-novel-application-of-rnase-h2-dependent-quantitative-pcr-for-detection-and-quantification-of-grosmannia-clavigera-a-mountain-pine-beetle-fungal-symbiont-in-environmental-samples
#9
Chandra H McAllister, Colleen E Fortier, Kate R St Onge, Bianca M Sacchi, Meaghan J Nawrot, Troy Locke, Janice E K Cooke
Mountain pine beetle (Dendroctonus ponderosae Hopkins; MPB) is an economically and ecologically important pest of pine species in western North America. Mountain pine beetles form complex multipartite relationships with microbial partners, including the ophiostomoid fungi Grosmannia clavigera (Robinson-Jeffrey and Davidson) Zipfel, de Beer and Wingfield, Ophiostoma montium (Rumbold) von Arx, Grosmannia aurea (Robinson-Jeffrey and Davidson) Zipfel, de Beer and Wingfield, Leptographium longiclavatum (Lee, Kim, and Breuil) and Leptographium terebrantis (Barras and Perry)...
January 10, 2018: Tree Physiology
https://www.readbyqxmd.com/read/29328547/blow-flies-as-urban-wildlife-sensors
#10
Constanze Hoffmann, Kevin Merkel, Andreas Sachse, Pablo Rodríguez, Fabian H Leendertz, Sébastien Calvignac-Spencer
Wildlife detection in urban areas is very challenging. Conventional monitoring techniques such as direct observation are faced with the limitation that urban wildlife is extremely elusive. It was recently shown that invertebrate-derived DNA (iDNA) can be used to assess wildlife diversity in tropical rainforests. Flies, which are ubiquitous and very abundant in most cities, may also be used to detect wildlife in urban areas. In urban ecosystems, however, overwhelming quantities of domestic mammal DNA could completely mask the presence of wild mammal DNA...
January 12, 2018: Molecular Ecology Resources
https://www.readbyqxmd.com/read/29327701/distribution-of-killer-immunoglobulin-like-receptor-genes-in-hiv-infected-long-term-non-progressors-from-mumbai-india
#11
Vijay R Chavan, Zakiya Ansari, Preeti Mehta, Jayanti Mania-Pramanik
BACKGROUND: Few reports suggest the association of killer immunoglobulin-like receptors of natural killer cells with human immunodeficiency virus infection. India with world's third largest population of human immunodeficiency virus / acquired immunodeficiency syndrome, offers scope to study such association. OBJECTIVE: Current study (2010-2015) was designed to evaluate if killer immunoglobulin-like receptors gene polymorphisms are associated with HIV infection outcomes specifically, with long term non progressors...
January 12, 2018: Indian Journal of Dermatology, Venereology and Leprology
https://www.readbyqxmd.com/read/29327589/transition-metal-dichalcogenide-nanosheets-for-visual-monitoring-pcr-rivaling-a-real-time-pcr-instrument
#12
Liu Wang, Zhicheng Huang, Rui Wang, Yibo Liu, Cheng Qian, Jian Wu, Juewen Liu
Monitoring the progress of polymerase chain reactions (PCR) is of critical importance in bioanalytical chemistry and molecular biology. While real-time PCR thermocyclers are ideal for this purpose, its high cost has limited applications in resource-poor areas. Direct visual detection would be a more attractive alternative. To monitor PCR amplification, DNA staining dyes such as SYBR Green I (SG) are often used. Although these dyes give higher fluorescence when binding to double-stranded (ds) DNA products, they also yield strong background fluorescence in the presence of a high concentration of single-stranded (ss) DNA primers...
January 12, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29327500/multiplex-polymerase-chain-reaction-detection-of-selected-bacterial-species-from-symptomatic-and-asymptomatic-non-vital-teeth-with-primary-endodontic-infections
#13
Smita Pattanshetty, Vijayalakshmi S Kotrashetti, Kishore Bhat, Ramakant S Nayak, Pradeep Somannavar, Madhu Pujar
AIM: The aim of the present study was to investigate the presence of selective anaerobic microorganisms in primary root canal infections of symptomatic and asymptomatic non-vital teeth with periapical pathosis using multiplex polymerase chain reaction. METHODS: A total of 100 root canal samples (50 from symptomatic and 50 from asymptomatic teeth) were obtained from patients with primary endodontic infections. DNA extracted from the samples was amplified by using specific primers for the 16S rRNA gene of each bacterium, and semiquantification was done to analyze the prevalence of microorganisms and their correlation to clinical features...
January 11, 2018: Journal of Investigative and Clinical Dentistry
https://www.readbyqxmd.com/read/29327293/overexpression-of-spot-gene-in-coccoid-forms-of-clinical-helicobacter-pylori-isolates
#14
Farkhondeh Poursina, Jamshid Fagri, Nasrin Mirzaei, Hajieh Ghasemian Safaei
Helicobacter pylori (H. pylori) can convert to coccoid form in unfavorable conditions or as a result of antibiotic treatment. In order to adapt to harsh environments, H. pylori requires a stringent response which, encoded by the spoT gene, has a bifunctional enzyme possessing both (p)ppGpp synthetic and degrading activity. Our goal in this study was to compare spoT gene expression in spiral and induced coccoid forms of H. pylori with use of amoxicillin. First, clinical isolate coccoid forms were induced with amoxicillin; then, the viability test was analyzed by flow cytometer...
January 11, 2018: Folia Microbiologica
https://www.readbyqxmd.com/read/29326512/evaluating-the-effect-of-different-conditioning-agents-on-the-shear-bond-strength-of-resin-modified-glass-ionomers
#15
Namith Rai, Rajaram Naik, Ravi Gupta, Shobana Shetty, Amith Singh
Aim of the Study: This study aims to evaluate the effects three different conditioning agents on the shear bond strength of resin-modified glass ionomers to human dentin. Materials and Methods: One hundred and twenty recently extracted, caries-free premolars and molars will be cleaned of debris and disinfected in a 0.5% solution of sodium hypochlorite and sterile water for 30 min. The occlusal surface of each tooth will be reduced using conventional model trimmer with water to produce the dentin surface...
October 2017: Contemporary Clinical Dentistry
https://www.readbyqxmd.com/read/29325701/developmental-cognitive-neuroscience-using-latent-change-score-models-a-tutorial-and-applications
#16
REVIEW
Rogier A Kievit, Andreas M Brandmaier, Gabriel Ziegler, Anne-Laura van Harmelen, Susanne M M de Mooij, Michael Moutoussis, Ian M Goodyer, Ed Bullmore, Peter B Jones, Peter Fonagy, Ulman Lindenberger, Raymond J Dolan
Assessing and analysing individual differences in change over time is of central scientific importance to developmental neuroscience. However, the literature is based largely on cross-sectional comparisons, which reflect a variety of influences and cannot directly represent change. We advocate using latent change score (LCS) models in longitudinal samples as a statistical framework to tease apart the complex processes underlying lifespan development in brain and behaviour using longitudinal data. LCS models provide a flexible framework that naturally accommodates key developmental questions as model parameters and can even be used, with some limitations, in cases with only two measurement occasions...
November 22, 2017: Developmental Cognitive Neuroscience
https://www.readbyqxmd.com/read/29324636/simple-sequence-repeat-ssr-genetic-linkage-map-of-d-genome-diploid-cotton-derived-from-an-interspecific-cross-between-gossypium-davidsonii-and-gossypium-klotzschianum
#17
Joy Nyangasi Kirungu, Yanfeng Deng, Xiaoyan Cai, Richard Odongo Magwanga, Zhongli Zhou, Xingxing Wang, Yuhong Wang, Zhenmei Zhang, Kunbo Wang, Fang Liu
The challenge in tetraploid cotton cultivars is the narrow genetic base and therefore, the bottleneck is how to obtain interspecific hybrids and introduce the germplasm directly from wild cotton to elite cultivars. Construction of genetic maps has provided insight into understanding the genome structure, interrelationships between organisms in relation to evolution, and discovery of genes that carry important agronomic traits in plants. In this study, we generated an interspecific hybrid between two wild diploid cottons, Gossypium davidsonii and Gossypium klotzschianum, and genotyped 188 F2:3 populations in order to develop a genetic map...
January 11, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29323936/sleep-research-a-primer-for-media-scholars
#18
Liese Exelmans, Jan Van den Bulck
The average amount of sleep people of all ages get has declined sharply in the past 50 years. The detrimental health effects of sleep deprivation are well documented and substantial. Even though electronic media use often takes place in the hours before sleep, the extent to which media use may interact with sleep is understudied and not well understood. Communication scholars are well positioned to contribute to this area, as a systematic, theoretical understanding of the relationship between media and sleep is still lacking...
January 11, 2018: Health Communication
https://www.readbyqxmd.com/read/29323541/development-of-a-targeted-next-generation-sequencing-assay-to-detect-diagnostically-relevant-mutations-of-jak2-calr-and-mpl-in-myeloproliferative-neoplasms
#19
Thomas Frawley, Cathal P O'Brien, Eibhlin Conneally, Elisabeth Vandenberghe, Melanie Percy, Stephen E Langabeer, Karl Haslam
BACKGROUND: The classical Philadelphia chromosome-negative myeloproliferative neoplasms (MPNs), consisting of polycythemia vera, essential thrombocythemia, and primary myelofibrosis, are a heterogeneous group of neoplasms that harbor driver mutations in the JAK2, CALR, and MPL genes. The detection of these mutations has been incorporated into the recent World Health Organization (WHO) diagnostic criteria for MPN. Given a pressing clinical need to screen for these mutations in a routine diagnostic setting, a targeted next-generation sequencing (NGS) assay for the detection of MPN-associated mutations located in JAK2 exon 14, JAK2 exon 12, CALR exon 9, and MPL exon 10 was developed to provide a single platform alternative to reflexive, stepwise diagnostic algorithms...
January 11, 2018: Genetic Testing and Molecular Biomarkers
https://www.readbyqxmd.com/read/29323478/simultaneous-detection-of-antibiotic-resistance-genes-on-paper-based-chip-using-ru-phen-2dppz-2-turn-on-fluorescence-probe
#20
Bofan Li, Xiaoming Zhou, Hongxing Liu, Huaping Deng, Ru Huang, Da Xing
Antibiotic resistance, the ability of some bacteria to resist antibiotic drug, has been a major global health burden due to the extensive use of antibiotic agents. Antibiotic resistance is encoded via respective genes, hence the specific detection of these genes is necessary for diagnostic and treat of antibiotic resistance cases. Conventional methods for monitoring antibiotic resistance genes require sample to be transported to a central laboratory for tedious and sophisticated tests, which is grueling and time-consuming...
January 11, 2018: ACS Applied Materials & Interfaces
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