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Hongge Jia, Yunzen Zhang, Vladimir Orbovic, Jin Xu, Frank White, Jeffrey Jones, Nian Wang
Citrus is a highly valued tree crop worldwide, while, at the same time, citrus production faces many biotic challenges, including bacterial canker and Huanglongbing (HLB). Breeding for disease resistant varieties is the most efficient and sustainable approach to control plant diseases. Traditional breeding of citrus varieties is challenging due to multiple limitations, including polyploidy, polyembryony, extended juvenility, and long crossing cycles. Targeted genome editing technology has the potential to shorten varietal development for some traits, including disease resistance...
December 9, 2016: Plant Biotechnology Journal
Yi-Lin Zhang, Jia-Wen Xu, Xiong-Hao Wu, Peng-Xiao Chen, Fang Wei, Dong-Dong Gao, Xiao-Wen Chen, Yu-Li Luo, Yun-Xin Zhu, Jiehong Huang, Ya-Xia Tan, Lei Zhao, Wen-Liang Zhou
Sodium tanshinone IIA sulphonate, a water-soluble derivative of tanshinone IIA, has been proven to possess versatile biological properties, but its pharmacological effect on tracheal smooth muscle remains elusive. This paper presents a study on the relaxant effect and underlying mechanisms of sodium tanshinone IIA sulphonate on mouse tracheal smooth muscle. The relaxant effect of sodium tanshinone IIA sulphonate was evaluated in mouse tracheal rings using a mechanical recording system. Intracellular Ca(2+) concentration was measured in primary cultured tracheal smooth muscle cells using confocal imaging system...
December 9, 2016: Planta Medica
Ming-Zhi Zhang, Chen-Yang Jia, Yu-Cheng Gu, Nick Mulholland, Sarah Turner, David Beattie, Wei-Hua Zhang, Guang-Fu Yang, John Clough
Based on examples of the successful applications in drug discovery of bioisosterism, a series of streptochlorin analogues in which indole has been replaced by other heterocycles has been designed and synthesized, as a continuation of our studies aimed at the discovery of novel streptochlorin analogues with improved antifungal activity. Biological testing showed that most of the indole-replaced streptochlorin analogues were inactive, though compound 6f had a broad spectrum of antifungal activity with significant activity against Alternaria solani...
December 2, 2016: European Journal of Medicinal Chemistry
Xiuqin Hong, Nualnong Wongtongkam, Paul Russell Ward, Shuiyuan Xiao, Shuling Wang, Qian Peng, Qi Zuo, Dan Zeng, Jia Wang, Chenxu Wang, Jian Chen, Na Zhang
Hypertension is a major risk factor for cardiovascular diseases in China; hence, identifying good serum markers might provide cost benefits in terms of reducing morbidity rates. In this population-based case-control study, participants were recruited from five districts in Hunan province, and 416 cases were matched with an equal number of controls. Markers related to elevated blood pressure were assessed: Body Mass Index, total cholesterol, triglycerides, fasting blood glucose, and creatinine. Three potential serum markers homocysteine (HCY), C-reactive protein (CRP), and alanine aminotransferase (ALT) were dichotomized as normal or high level...
December 9, 2016: Clinical and Experimental Hypertension: CHE
Yang Xiaoling, Zhao Li, Li ShuQiang, Ma Shengchao, Yang Anning, Ding Ning, Li Nan, Jia Yuexia, Yang Xiaoming, Li Guizhong, Jiang Yideng
Hyperhomocysteinemia (HHcy) is an independent risk factor for cardiovascular diseases, such as atherosclerosis. HHcy promotes atherogenesis by modifying the histone methylation patterns and miRNA regulation. In this study, we investigated the effects of homocysteine (Hcy) on the expression of enhancer of zeste homolog 2 (EZH2), and tested our hypothesis that Hcy-induced atherosclerosis is mediated by increased EZH2 expression, which is regulated by miR-92a. The levels of EZH2 and H3K27me3 were increased in the aorta of ApoE-/- mice fed a high-methionine diet for 16 weeks, whereas miR-92a expression was decreased...
2016: PloS One
Cunling Jia, Xiaoyan Kong, James E Koltes, Xiao Gou, Shuli Yang, Dawei Yan, Shaoxiong Lu, Zehui Wei
Tibetan pigs have survived at high altitude for millennia and they have a suite of adaptive features to tolerate the hypoxic environment. However, the molecular mechanisms underlying the regulation of hypoxia-adaptive phenotypes have not been completely elucidated. In this study, we analyzed differentially expressed genes (DEGs), biological pathways and constructed co-expression regulation networks using whole-transcriptome microarrays from lung tissues of Tibetan and Duroc pigs both at high and low altitude...
2016: PloS One
Weiwei Yang, Anning Wang, Chunling Zhao, Qinghua Li, Zhifang Pan, Xuefu Han, Cuijuan Zhang, Guohui Wang, Chao Ji, Guili Wang, Guangtao Jia, Jiyu Ju, Wei Gao, Wenjing Yu, Xiaoying Liu, Xi Chen, Weiguo Feng, Zhiqin Gao, Jie Li, Chune Ren
Preeclampsia (PE) is one of the leading causes of maternal and perinatal mortality and morbidity. One of the main hallmarks observed in PE is impaired inflammation state. In the current study, we found that miR-125b was deregulated in placental tissues and plasma derived from PE patients, which suggest a potential association between this miRNA and the pathogenesis of PE. Overexpression of miR-125b significantly reduced SGPL1 expression, and luciferase assays confirmed that SGPL1 is a direct target of miR-125b...
2016: PloS One
Ying Zhou, Nan Song, Xin Li, Ying Han, Zihan Ren, Jing-Xian Xu, Yu-Chen Han, Fang Li, Xinshan Jia
We investigated the relationship between promoter methylation and tracheal stem cell activation. We developed a model of rat tracheal epithelium regeneration after 5-fluorouracil (5-FU)-induced injury. Using immunohistochemistry and Western blotting, the expression levels of the stem cell pluripotency regulator Oct3/4 and differentiation marker CK14 were measured after 5-FU treatment. The methylation status of the Oct3/4, Nanog, and Sox2 promoters was investigated using methylation-specific PCR. Additionally, the effects of 5-azacytidine (5-azaC), a demethylating agent, on Oct3/4, Nanog, and Sox2 mRNA and protein expression were evaluated...
December 7, 2016: Oncotarget
Jia Chen, Theodore S Jardetzky, Richard Longnecker
: Epstein-Barr virus (EBV) is associated with infectious mononucleosis and a variety of cancers as well as lymphoproliferative disorders in immunocompromised patients. EBV mediates viral entry into epithelial and B cells using fusion machinery composed of four glycoproteins: gB, the gH/gL complex, and gp42. gB and gH/gL are required for both epithelial and B cell fusion. The specific role of gH/gL in fusion has been the most elusive among the required herpesvirus entry glycoproteins. Previous mutational studies have focused on the ectodomain of EBV gH and not on the gH cytoplasmic tail domain (CTD)...
November 15, 2016: MBio
Yakun Tang, Lang Liu, Hongyang Zhao, Shasha Gao, Yan Lv, Lingbing Kong, Junhong Ma, Dianzeng Jia
Compared with Li4Ti5O12, LiCrTiO4 with the same spinel structure exhibits superior conductivity and Li(+) diffusion properties. However, there has been no extensive study on LiCrTiO4 as an anode material in lithium ion batteries, due to its pulverization, loss of electrical contact, and particle aggregation. A unique architecture based on hybrid porous CNTs embedding ultrasmall LiCrTiO4 nanoparticles (6 ± 2 nm) was designed by using a facile sol-gel process combined with subsequent heat treatment. As an anode for lithium ion batteries, due to the absence of the aforementioned problems, the electrode exhibited high reversible capacity, excellent rate capability and superior long-term cycling stability at high current densities...
December 9, 2016: Chemical Communications: Chem Comm
Pin Chieh Wu, Wei-Yi Tsai, Wei Ting Chen, Yao-Wei Huang, Ting-Yu Chen, Jia-Wern Chen, Chun Yen Liao, Cheng Hung Chu, Greg Sun, Din Ping Tsai
All forms of light manipulation rely on light-matter interaction, the primary mechanism of which is the modulation of its electromagnetic fields by the localized electromagnetic fields of atoms. One of the important factors that influence the strength of interaction is the polarization of the electromagnetic field. The generation and manipulation of light polarization have been traditionally accomplished with bulky optical components such as waveplates, polarizers, and polarization beam splitters that are optically thick...
December 8, 2016: Nano Letters
Feng-Qin Wang, Qiao-Qiao Li, Qian Zhang, Yin-Zhen Wang, Yuan-Jia Hu, Peng Li, Jian-Bo Wan, Feng-Qing Yang, Zhi-Ning Xia
In this study, the affinity interactions between RAW 264.7 macrophages and three small molecules include naringin, oleuropein and paeoniflorin were evaluated by affinity capillary electrophoresis (ACE), partial filling affinity capillary electrophoresis (PFACE) and frontal analysis capillary electrophoresis (FACE), respectively. The result indicated that 1) ACE (varying concentrations of cell suspension were filled in the capillary as receptor) may not be suitable for the evaluation of interactions between cell and small molecules due to the high viscosity of cell suspension; 2) PFACE can qualitatively evaluate the interaction, but the difference in viscosity between RAW264...
December 9, 2016: Electrophoresis
Zhong-Yi Yan, Zhi-Qing Luo, Lai-Jian Zhang, Jia Li, Jia-Qiang Liu
MicroRNAs have been used as diagnostic and prognostic biomarkers for many cancers including oral squamous cell carcinoma (OSCC). Several studies have been shown that microRNA (miRNA) play important roles during the progression of OSCC. However, the results vary largely in different studies due to different platforms and sample sizes. In this study, we systematically evaluated a large scale of miRNA profiles from current qualified OSCC samples, and further investigated the functions of genes regulated by these key miRNAs as well as the signaling pathways through which these miRNA effect carcinogenesis...
December 9, 2016: Journal of Cellular Physiology
Peng Zhou, Yok Teng Chionh, Sergio Erdal Irac, Matae Ahn, Justin Han Jia Ng, Even Fossum, Bjarne Bogen, Florent Ginhoux, Aaron T Irving, Charles-Antoine Dutertre, Lin-Fa Wang
Bats carry and shed many emerging infectious disease agents including Ebola virus and SARS-like Coronaviruses, yet they rarely display clinical symptoms of infection. Bat epithelial or fibroblast cell lines were previously established to study the bat immune response against viral infection. However, the lack of professional immune cells such as dendritic cells (DC) and macrophages has greatly limited the significance of current investigations. Using Pteropus alecto (P. alecto) GM-CSF plus IL4, FLT3L and CSF-1, we successfully generated bat bone marrow-derived DC and macrophages...
December 9, 2016: Scientific Reports
Hu Qiao, Bo Hu, Hong Zhou, Jianqun Yan, Ru Jia, Bo Lu, Bo Sun, Xiao Luo, Yuanyuan Fan, Nan Wang
The purpose of this study was to determine whether aldosterone has a rapid action in the nucleus tractus solitarius (NTS) that increases sodium intake, and to examine whether this effect of aldosterone, if present, is mediated by G protein-coupled estrogen receptor (GPER). Adult male Sprague-Dawley rats with a stainless-steel cannula in the NTS were used. Aldosterone was injected into the NTS at the doses of 1, 5, 10 and 20 ng 0.1 μl(-1). A rapid dose-related increase of 0.3 M NaCl intake was induced within 30 min and this increase was not suppressed by the mineralocorticoid receptor (MR) antagonist spironolactone (10 ng 0...
December 9, 2016: Scientific Reports
Shengjun Tan, Margarida Cardoso-Moreira, Wenwen Shi, Dan Zhang, Jiawei Huang, Yanan Mao, Hangxing Jia, Yaqiong Zhang, Chunyan Chen, Yi Shao, Liang Leng, Zhonghua Liu, Xun Huang, Manyuan Long, Yong E Zhang
In a broad range of taxa, genes can duplicate through an RNA intermediate in a process mediated by retrotransposons (retroposition). In mammals, L1 retrotransposons drive retroposition, but the elements responsible for retroposition in other animals have yet to be identified. Here, we examined young retrocopies from various animals that still retain the sequence features indicative of the underlying retroposition mechanism. In Drosophila melanogaster, we identified and de novo assembled 15 polymorphic retrocopies and found that all retroposed loci are chimeras of internal retrocopies flanked by discontinuous LTR retrotransposons...
December 2016: Genome Research
Jia Yu Ng, Richard Stirling
No abstract text is available yet for this article.
December 1, 2016: Postgraduate Medical Journal
Lin Zhou, Kun Wang, Zhenhua Wu, Haidao Dong, Hao Sun, Xuanhong Cheng, Hong Lian Zhang, Hongbo Zhou, Chunping Jia, Qinghui Jin, Hongju Mao, Jean-Luc Coll, Jianlong Zhao
Two-dimensional graphene devices are widely used for biomolecule detection. Nevertheless, the surface modification of graphene is critical to achieve the high sensitivity and specificity required for biological detection. Herein, native bovine serum albumin (BSA) in inorganic solution is denatured on the graphene surface by heating, leading to the formation of nanoscale BSA protein films adsorbed on the graphene substrate via π-stacking interactions. This technique yields a controllable, scalable, uniform, and high-coverage method for graphene biosensors...
December 6, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
Rong-Jia Wei, Takuya Shiga, Graham N Newton, David Robinson, Sadamu Takeda, Hiroki Oshio
A cage complex, H2O@[Co5Fe4], was found to encapsulate two water molecules during its self-assembly. The complex exhibited remarkable multifunctionality, combining magnetic switching from the thermal electron-transfer-coupled spin transition of the cage host and dipolar reorientational motion of the confined water, as evidenced by permittivity measurements, density functional theory calculations, and solid-state (2)H NMR spectra.
December 5, 2016: Inorganic Chemistry
Jia Yuan, Xinping Long, Chaoyang Zhang
N-Oxidization is an important strategy for enhancing the density and energy of energetic materials. Nevertheless, the influence of N(+)-O(-) introduction on molecular stability remains relatively unknown. Thus, the present work comprehensively studied 102 basic N-rich ring structures, including azoles, furazans, and azines, as well as their N-oxides by quantum chemical calculations. The introduction of N(+)-O(-) weakens molecular stability in most cases because the process elongates chemical bonds, decreases ring aromaticity, narrows the gaps between the highest occupied and lowest unoccupied molecular orbitals, and increases the photochemical reactivity...
December 1, 2016: Journal of Physical Chemistry. A
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