keyword
https://read.qxmd.com/read/36657776/doctors-pay-review-body-must-be-made-truly-independent-says-bma
#1
JOURNAL ARTICLE
Gareth Iacobucci
No abstract text is available yet for this article.
January 19, 2023: BMJ: British Medical Journal
https://read.qxmd.com/read/35074587/role-of-conserved-regulatory-loop-residues-in-allosteric-propagation-of-serine-protease-htra2
#2
JOURNAL ARTICLE
Aasna L Parui, Nitu Singh, Shubhankar Dutta, Kakoli Bose
High temperature requirement protease A2 (HtrA2) is a mitochondrial serine protease that demonstrates multifaceted roles including protein quality control and proapoptotic properties in humans, making it a potential therapeutic target. Current literature suggests involvement of flexible regulatory loops in governing the allosteric propagation within the trimeric HtrA2 ensemble. Here, we have identified three important residues - R147, P148 (L3 loop) and F131 (LD loop) surrounding the catalytic-site that play crucial roles in stabilizing HtrA2 active conformation during its multimodal activation...
January 19, 2022: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/30946901/molecular-basis-for-feedback-inhibition-of-tyrosine-regulated-3-deoxy-d-arabino-heptulosonate-7-phosphate-synthase-from-escherichia-coli
#3
JOURNAL ARTICLE
Di Cui, Aihua Deng, Hua Bai, Zhao Yang, Yong Liang, Zhe Liu, Qidi Qiu, Laiyou Wang, Shuwen Liu, Yun Zhang, Yi Shi, Jianxun Qi, Tingyi Wen
3-deoxy-d-arabino-heptulosonate-7-phosphate synthase (DAHPS) is responsible for the biosynthesis of essential aromatic compounds in microorganisms and plants. It plays a crucial role in the regulation of the carbon flow into the shikimate pathway. Until now, the crystal structures and regulatory mechanisms of dimeric DAHPS enzymes from type Iα subclass have not been reported. Here, we reported dimeric structures of the tyrosine-regulated DAHPS from Escherichia coli, both in its apo form and complex with the inhibitor tyrosine at 2...
April 1, 2019: Journal of Structural Biology
https://read.qxmd.com/read/28175030/p148-fecal-calprotectin-as-a-noninvasive-indicator-for-ulcerative-colitis-disease-activity-in-the-korean-cohort
#4
JOURNAL ARTICLE
S-H Lee, H Seo, K Chang, E M Song, G-U Kim, M Seo, E J Kwon, S W Hwang, S H Park, D-H Yang, K-J Kim, J-S Byeon, S-J Myung, S-K Yang, B D Ye
No abstract text is available yet for this article.
February 1, 2017: Journal of Crohn's & Colitis
https://read.qxmd.com/read/27558264/mmp20-proteolysis-of-native-amelogenin-regulates-mineralization-in-vitro
#5
JOURNAL ARTICLE
S Y Kwak, Y Yamakoshi, J P Simmer, H C Margolis
Recent studies have shown that native phosphorylated full-length porcine amelogenin (P173) and its predominant cleavage product (P148) can inhibit spontaneous calcium phosphate formation in vitro by stabilizing an amorphous calcium phosphate (ACP) precursor phase. Since full-length amelogenin undergoes proteolysis by matrix metalloproteinase 20 (MMP20, enamelysin) soon after secretion, the present study was conducted to assess the effect of amelogenin proteolysis on calcium phosphate formation. Calcium and phosphate were sequentially added to protein solutions without and with added MMP20 (ratio = 200:1) under physiological-like conditions of ionic strength (163 mM) in 50 mM Tris-HCl (pH 7...
December 2016: Journal of Dental Research
https://read.qxmd.com/read/26446598/characterizing-the-murine-leukemia-virus-envelope-glycoprotein-membrane-spanning-domain-for-its-roles-in-interface-alignment-and-fusogenicity
#6
JOURNAL ARTICLE
Daniel J Salamango, Marc C Johnson
UNLABELLED: The membrane-proximal region of murine leukemia virus envelope (Env) is a critical modulator of its functionality. We have previously shown that the insertion of one amino acid (+1 leucine) within the membrane-spanning domain (MSD) abolished protein functionality in infectivity assays. However, functionality could be restored to this +1 leucine mutant by either inserting two additional amino acids (+3 leucine) or by deleting the cytoplasmic tail domain (CTD) in the +1 leucine background...
December 2015: Journal of Virology
https://read.qxmd.com/read/25869657/production-and-characterization-of-monospecific-and-bispecific-antibodies-against-dengue-virus-ns1-protein
#7
JOURNAL ARTICLE
Advaita Ganguly, Ravindra B Malabadi, Pravin K Bhatnagar, Xinli Tang, Dipankar Das, Raimer Loebenberg, Mavanur R Suresh, Hoon H Sunwoo
Dengue is a mosquito borne infection, which in recent years has become a major international public health concern. Annually, 100 million dengue virus infections are reported worldwide. The nonstructural protein 1 (NS1) of dengue virus is a useful target for diagnostics of dengue infection since the protein is abundantly circulating in the blood during acute phase of the disease, in both primary and secondary infections. This research paper highlights the development of a panel of Mab and bsMab for dengue NS1 detection...
August 2015: Journal of Virological Methods
https://read.qxmd.com/read/25717611/p148-immunohistochemical-staining-of-plasma-cells-and-eosinophils-in-inflammatory-bowel-disease-a-preliminary-report
#8
JOURNAL ARTICLE
(no author information available yet)
No abstract text is available yet for this article.
February 2015: Journal of Crohn's & Colitis
https://read.qxmd.com/read/25527438/improvement-of-catechin-production-in-escherichia-coli-through-combinatorial-metabolic-engineering
#9
JOURNAL ARTICLE
Shujuan Zhao, J Andrew Jones, Daniel M Lachance, Namita Bhan, Omar Khalidi, Sylesh Venkataraman, Zhengtao Wang, Mattheos A G Koffas
Reconstruction of highly efficient biosynthesis pathways is essential for the production of valuable plant secondary metabolites in recombinant microorganisms. In order to improve the titer of green tea catechins in Escherichia coli, combinatorial strategies were employed using the ePathBrick vectors to express the committed catechin pathway: flavanone 3β-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR), and leucoanthocyanidin reductase (LAR). Three F3H, three DFR, and two LAR genes originating from different plant species were selected and synthesized, to create 18 pathway variants to be screened in E...
March 2015: Metabolic Engineering
https://read.qxmd.com/read/25261469/g-protein-and-%C3%AE-arrestin-signaling-bias-at-the-ghrelin-receptor
#10
JOURNAL ARTICLE
Tama Evron, Sean M Peterson, Nikhil M Urs, Yushi Bai, Lauren K Rochelle, Marc G Caron, Larry S Barak
The G protein-coupled ghrelin receptor GHSR1a is a potential pharmacological target for treating obesity and addiction because of the critical role ghrelin plays in energy homeostasis and dopamine-dependent reward. GHSR1a enhances growth hormone release, appetite, and dopamine signaling through G(q/11), G(i/o), and G(12/13) as well as β-arrestin-based scaffolds. However, the contribution of individual G protein and β-arrestin pathways to the diverse physiological responses mediated by ghrelin remains unknown...
November 28, 2014: Journal of Biological Chemistry
https://read.qxmd.com/read/25181238/-p148-the-use-of-updated-dosimetric-guidelines-and-independent-dose-volume-histogram-analysis-improves-treatment-planning-for-men-treated-with-rapidarc-intensity-modulated-radiation-therapy-for-clinically-localized-prostate-cancer
#11
JOURNAL ARTICLE
https://read.qxmd.com/read/25158174/regulation-of-calcium-phosphate-formation-by-native-amelogenins-in-vitro
#12
JOURNAL ARTICLE
Seo-Young Kwak, Sonia Kim, Yasuo Yamakoshi, James P Simmer, Elia Beniash, Henry C Margolis
Our previous in vitro studies have shown that recombinant full-length porcine amelogenin rP172 can transiently stabilize amorphous calcium phosphate (ACP) and uniquely guide the formation of well-aligned bundles of hydroxyapatite (HA) crystals, as seen in the secretory stage of amelogenesis. This functional capacity is dependent on the hydrophilic C-terminal domain of full-length amelogenin. However, we have also found that native phosphorylated (single S-16 site) forms of full-length (P173) and C-terminal cleaved (P148) amelogenins can stabilize ACP for > 2 d and prevent HA formation...
August 2014: Connective Tissue Research
https://read.qxmd.com/read/25024114/micrornas-as-potential-biomarkers-for-vero-cell-tumorigenicity
#13
JOURNAL ARTICLE
Belete Teferedegne, Juliete Macauley, Gideon Foseh, Eugenia Dragunsky, Konstantin Chumakov, Haruhiko Murata, Keith Peden, Andrew M Lewis
MicroRNA expression appears to capture the process of neoplastic development in vitro in the VERO line of African green monkey kidney (AGMK) cells (Teferedegne et al. PLoS One 2010;5(12):e14416). In that study, specific miRNA signatures were correlated with the transition, during serial tissue-culture passage, of low-density passaged 10-87 VERO cells from a non-tumorigenic phenotype at passage (p) 148 to a tumorigenic phenotype at p256. In the present study, six miRNAs (miR-376a, miR-654-3p, miR-543, miR-299-3p, miR-134 and miR-369-3p) were chosen from the identified signature miRNAs for evaluation of their use as potential biomarkers to track the progression of neoplastic development in VERO cells...
August 20, 2014: Vaccine
https://read.qxmd.com/read/22243235/regulation-of-calcium-phosphate-formation-by-amelogenins-under-physiological-conditions
#14
JOURNAL ARTICLE
Seo-Young Kwak, Samantha Green, Felicitas B Wiedemann-Bidlack, Elia Beniash, Yasuo Yamakoshi, James P Simmer, Henry C Margolis
Amelogenin is essential for proper enamel formation. The present in vitro study extends our previous work at low (10 mM) ionic strength (IS) by examining the effect of amelogenin on mineralization under higher (162 mM) IS conditions found in developing enamel. Full-length phosphorylated (P173) and non-phosphorylated (rP172) amelogenins were examined, along with P148 and rP147 that lack the hydrophilic C-terminus. Calcium phosphate formation was assessed by pH change, while the minerals formed were characterized using transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy...
December 2011: European Journal of Oral Sciences
https://read.qxmd.com/read/22200995/porcine-amelogenin-alternative-splicing-proteolytic-processing-protein-protein-interactions-and-possible-functions
#15
Yasuo Yamakoshi
Amelogenin is the major secretory product of ameloblasts and is critical for proper tooth enamel formation. Amelogenin isoforms and their cleavage products comprise over 80% of total secretory stage enamel protein. We have isolated and characterized four secreted amelogenin isoforms from developing porcine enamel : P190 (27-kDa), P173 (25-kDa), P132 (18-kDa) and P56 (6.5-kDa ; leucine rich amelogenin polypeptide or LRAP). P190 and P132 are low abundance amelogenins that contain a novel exon 4-encoded segment of lack the exon 3-encoded segment, respectively...
2011: Journal of Oral Biosciences
https://read.qxmd.com/read/21074619/effects-of-phosphorylation-on-the-self-assembly-of-native-full-length-porcine-amelogenin-and-its-regulation-of-calcium-phosphate-formation-in-vitro
#16
JOURNAL ARTICLE
Felicitas B Wiedemann-Bidlack, Seo-Young Kwak, Elia Beniash, Yasuo Yamakoshi, James P Simmer, Henry C Margolis
The self-assembly of the predominant extracellular enamel matrix protein amelogenin plays an essential role in regulating the growth and organization of enamel mineral during early stages of dental enamel formation. The present study describes the effect of the phosphorylation of a single site on the full-length native porcine amelogenin P173 on self-assembly and on the regulation of spontaneous calcium phosphate formation in vitro. Studies were also conducted using recombinant non-phosphorylated (rP172) porcine amelogenin, along with the most abundant amelogenin cleavage product (P148) and its recombinant form (rP147)...
February 2011: Journal of Structural Biology
https://read.qxmd.com/read/19443653/role-of-20-kda-amelogenin-p148-phosphorylation-in-calcium-phosphate-formation-in-vitro
#17
JOURNAL ARTICLE
Seo-Young Kwak, Felicitas B Wiedemann-Bidlack, Elia Beniash, Yasuo Yamakoshi, James P Simmer, Amy Litman, Henry C Margolis
The potential role of amelogenin phosphorylation in enamel formation is elucidated through in vitro mineralization studies. Studies focused on the native 20-kDa porcine amelogenin proteolytic cleavage product P148 that is prominent in developing enamel. Experimental conditions supported spontaneous calcium phosphate precipitation with the initial formation of amorphous calcium phosphate (ACP). In the absence of protein, ACP was found to undergo relatively rapid transformation to randomly oriented plate-like apatitic crystals...
July 10, 2009: Journal of Biological Chemistry
https://read.qxmd.com/read/17719243/ph-triggered-self-assembly-of-native-and-recombinant-amelogenins-under-physiological-ph-and-temperature-in-vitro
#18
JOURNAL ARTICLE
Felicitas B Wiedemann-Bidlack, Elia Beniash, Yasuo Yamakoshi, James P Simmer, Henry C Margolis
Self-assembly of the extracellular matrix protein amelogenin is believed to play an essential role in regulating the growth and organization of enamel crystals during enamel formation. This study examines the effect of temperature and pH on amelogenin self-assembly under physiological pH conditions in vitro, using dynamic light scattering, turbidity measurements, and transmission electron microscopy. Full-length recombinant amelogenins from mouse (rM179) and pig (rP172) were investigated, along with proteolytic cleavage products (rM166 and native P148) lacking the hydrophilic C-terminus of parent molecules...
October 2007: Journal of Structural Biology
https://read.qxmd.com/read/16674702/co-operative-mineralization-and-protein-self-assembly-in-amelogenesis-silica-mineralization-and-assembly-of-recombinant-amelogenins-in-vitro
#19
JOURNAL ARTICLE
Christabel E Fowler, Elia Beniash, Yasuo Yamakoshi, James P Simmer, Henry C Margolis
An amorphous silica mineralization technique was used to produce inorganic/protein composites to elucidate the structure and mechanism of formation of amelogenin assemblies, which may play an important role in regulating enamel structure during the initial stages of amelogenesis. Full-length recombinant amelogenins from mouse (rM179) and pig (rP172) were investigated along with key degradation products (rM166 and native P148) lacking the hydrophilic C terminus found in parent molecules. The resulting products were examined using transmission electron microscopy and/or small-angle X-ray scattering...
May 2006: European Journal of Oral Sciences
https://read.qxmd.com/read/12489197/limited-proteolysis-of-amelogenin-toward-understanding-the-proteolytic-processes-in-enamel-extracellular-matrix
#20
REVIEW
J Moradian-Oldak, N Gharakhanian, I Jimenez
This article is a short review of our recent study on controlled proteolysis of amelogenins by a series of commercially available proteinases as well as the tooth-specific metalloproteinase enamelysin. A limited proteolysis approach and mass spectrometry were applied in order to determine the surface accessibility of conserved domains of amelogenin nanospheres. Furthermore, this study was aimed at exploring the factors that affect the activity of enamel proteases to process amelogenins and at providing insight into the mechanisms of amelogenin degradation during amelogenesis...
2002: Connective Tissue Research
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