keyword
MENU ▼
Read by QxMD icon Read
search

Syntaxin

keyword
https://www.readbyqxmd.com/read/29655575/crmp2-neurofibromin-interface-drives-nf1-related-pain
#1
Aubin Moutal, Li Sun, Xiaofang Yang, Wennan Li, Song Cai, Shizhen Luo, Rajesh Khanna
An understudied symptom of the genetic disorder Neurofibromatosis type 1 (NF1) is chronic idiopathic pain. We used targeted editing of Nf1 in rats to provide direct evidence of a causal relationship between neurofibromin, the protein product of the Nf1 gene, and pain responses. Our study data identified a protein-interaction network with collapsin response meditator protein 2 (CRMP2) as a node and neurofibromin, syntaxin 1A, and the N-type voltage-gated calcium (CaV2.2) channel as interaction edges. Neurofibromin uncouples CRMP2 from syntaxin 1A...
April 12, 2018: Neuroscience
https://www.readbyqxmd.com/read/29623065/-in-situ-proximity-ligation-assay-reveals-co-localization-of-alpha-synuclein-and-snare-proteins-in-murine-primary-neurons
#2
Leire Almandoz-Gil, Emma Persson, Veronica Lindström, Martin Ingelsson, Anna Erlandsson, Joakim Bergström
The aggregation of alpha-synuclein (αSyn) is the pathological hallmark of Parkinson's disease, dementia with Lewy bodies and related neurological disorders. However, the physiological function of the protein and how this function relates to its pathological effects remain poorly understood. One of the proposed roles of αSyn is to promote the soluble N -ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex assembly by binding to VAMP-2. The objective of this study was to visualize the co-localization between αSyn and the SNARE proteins (VAMP-2, SNAP-25, and syntaxin-1) for the first time using in situ proximity ligation assay (PLA)...
2018: Frontiers in Neurology
https://www.readbyqxmd.com/read/29615512/-phytophthora-palmivora-establishes-tissue-specific-intracellular-infection-structures-in-the-earliest-divergent-land-plant-lineage
#3
Philip Carella, Anna Gogleva, Marta Tomaselli, Carolin Alfs, Sebastian Schornack
The expansion of plants onto land was a formative event that brought forth profound changes to the earth's geochemistry and biota. Filamentous eukaryotic microbes developed the ability to colonize plant tissues early during the evolution of land plants, as demonstrated by intimate, symbiosis-like associations in >400 million-year-old fossils. However, the degree to which filamentous microbes establish pathogenic interactions with early divergent land plants is unclear. Here, we demonstrate that the broad host-range oomycete pathogen Phytophthora palmivora colonizes liverworts, the earliest divergent land plant lineage...
April 3, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29599294/munc18-2-but-not-munc18-1-or-munc18-3-controls-compound-and-single-vesicle-regulated-exocytosis-in-mast-cells
#4
Berenice A Gutierrez, Miguel A Chavez, Alejandro I Rodarte, Marco Ramos, Andrea Dominguez, Youlia Petrova, Alfredo J Davalos, Renan M Costa, Ramon Elizondo, Michael J Tuvim, Burton F Dickey, Alan R Burns, Ruth Heidelberger, Roberto Adachi
Mast cells (MCs) play pivotal roles in many inflammatory conditions including infections, anaphylaxis and asthma. MCs store immunoregulatory compounds in their large cytoplasmic granules and, upon stimulation, secrete them via regulated exocytosis. Exocytosis in many cells requires the participation of Munc18 proteins (also known as syntaxin-binding proteins), and we found that mature MCs express all three mammalian isoforms: Munc18 1, 2 and 3. To study their functions in MC effector responses and test the role of MC degranulation in anaphylaxis, we used conditional knockout (cKO) mice in which each Munc18 protein was deleted exclusively in MCs...
March 29, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29564371/tracking-endocytosis-and-intracellular-trafficking-of-epitope-tagged-syntaxin-3-by-antibody-feeding-in-live-polarized-mdck-cells
#5
Adrian J Giovannone, Elena Reales, Pallavi Bhattaram, Alberto Fraile-Ramos, Thomas Weimbs
The uptake and trafficking of cell surface receptors can be monitored by a technique called 'antibody-feeding' which uses an externally applied antibody to label the receptor on the surface of cultured, live cells. Here, we adapt the traditional antibody-feeding experiment to polarized epithelial cells (Madin-Darby Canine Kidney) grown on permeable Transwell supports. By adding two tandem extracellular Myc epitope tags to the C-terminus of the SNARE protein syntaxin 3 (Stx3), we provided a site where an antibody could bind, allowing us to perform antibody-feeding experiments on cells with distinct apical and basolateral membranes...
February 5, 2018: Bio-protocol
https://www.readbyqxmd.com/read/29556948/duodeno-jejunal-bypass-restores-%C3%AE-cell-hypersecretion-and-islet-hypertrophy-in-western-diet-obese-rats
#6
Mariana Carla Mendes, Maria Lúcia Bonfleur, Rosane Aparecida Ribeiro, Camila Lubaczeuski, Ana Flavia Justino Fêo, Rodrigo Vargas, Everardo Magalhães Carneiro, Antonio Carlos Boschero, Allan Cezar Faria Araujo, Sandra Lucinei Balbo
PURPOSE: Duodeno-jejunal bypass (DJB) operation improves glucose homeostasis in morbid obesity, independently of weight loss or reductions in adiposity, through mechanisms not yet fully elucidated. Herein, we evaluated the effects of DJB upon glucose homeostasis, endocrine pancreatic morphology, and β-cell responsiveness to potentiating agents of cholinergic and cAMP pathways, in western diet (WD) obese rats, at 2 months after operation. METHODS: From 8 to 18 weeks of age male Wistar rats fed on a WD...
March 19, 2018: Endocrine
https://www.readbyqxmd.com/read/29554593/mir-34a-5p-mediates-sevoflurane-preconditioning-induced-inhibition-of-hypoxia-reoxygenation-injury-through-stx1a-in-cardiomyocytes
#7
Li Wenlan, Xia Zhongyuan, Lei Shaoqing, Zhan Liying, Zhao Bo, Liu Min
Anesthetic preconditioning is a cellular protective approach whereby exposure to a volatile anesthetic renders cardio injury. Sevoflurane preconditioning has been shown to exhibit cardio protective effect on hypoxia/reoxygenation (H/R) injury, but the underlying mechanism is unclear. Syntaxin 1A (STX1A), an important regulator in cardio disease, was predicted to be the target gene of microRNA-34a-5p (miR-34a-5p). The current research was designed to delineate the role of miR-34a-5p in regulating sevoflurane preconditioning in cardiomyocytes injury...
March 16, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29549124/kv2-1-clusters-on-%C3%AE-cell-plasma-membrane-act-as-reservoirs-that-replenish-pools-of-newcomer-insulin-granule-through-their-interaction-with-syntaxin-3
#8
Dafna Greitzer-Antes, Li Xie, Tairan Qin, Huanli Xie, Dan Zhu, Subhankar Dolai, Tao Liang, Fei Kang, Alexandre B Hardy, Yan He, Youhou Kang, Herbert Y Gaisano
The voltage-dependent K+ (Kv) channel Kv2.1 is a major delayed rectifier in many secretory cells, including pancreatic β-cells. In addition, Kv2.1 has a direct role in exocytosis at an undefined step, involving SNARE proteins and independent of its ion-conducting pore function. Here, we elucidated the precise step in exocytosis. We previously reported that syntaxin 3 (Syn-3) is the key syntaxin that mediates exocytosis of newcomer secretory granules that spend minimal residence time on the plasma membrane before fusion...
March 16, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29549094/syntaxin-17-promotes-lipid-droplet-formation-by-regulating-the-distribution-of-acyl-coa-synthetase-3
#9
Hana Kimura, Kohei Arasaki, Yuki Ohsaki, Toyoshi Fujimoto, Takayuki Ohtomo, Junji Yamada, Mitsuo Tagaya
Lipid droplets (LDs) are ubiquitous organelles that contain neutral lipids and are surrounded by a phospholipid monolayer. How proteins specifically localize to the phospholipid monolayer of the LD surface has been a matter of extensive investigations. In the present study we show that syntaxin 17 (Stx17), a SNARE protein whose expression in the liver is regulated by diet, participates in LD biogenesis by regulating the distribution of acyl-CoA synthetase 3 (ACSL3), a key enzyme for LD biogenesis that redistributes from the endoplasmic reticulum to LDs during LD formation...
March 16, 2018: Journal of Lipid Research
https://www.readbyqxmd.com/read/29548989/snap-25a-and-snap-25b-differently-mediate-interactions-with-munc18-1-and-g%C3%AE-%C3%AE-subunits
#10
Teresa Daraio, Ismael Valladolid-Acebes, Kerstin Brismar, Christina Bark
SNAP-25 is a protein involved in regulated membrane fusion and part of the SNARE complex. It exists as two splicing variants, SNAP-25a and SNAP-25b, which differ in 9 out of 206 amino acids. SNAP-25 together with Syntaxin 1 and VAMP-2 forms the ternary SNARE complex essential for mediating activity-dependent release of hormones and neurotransmitters. The functional difference between SNAP-25a and SNAP-25b is poorly understood as both can participate in SNARE complexes and mediate membrane fusion. However, we recently demonstrated that SNAP-25b-deficiency results in metabolic disease and increased insulin secretion...
March 13, 2018: Neuroscience Letters
https://www.readbyqxmd.com/read/29548366/solution-nmr-of-snares-complexin-and-%C3%AE-synuclein-in-association-with-membrane-mimetics
#11
REVIEW
Binyong Liang, Lukas K Tamm
SNARE-mediated membrane fusion is a ubiquitous process responsible for intracellular vesicle trafficking, including membrane fusion in exocytosis that leads to hormone and neurotransmitter release. The proteins that facilitate this process are highly dynamic and adopt multiple conformations when they interact with other proteins and lipids as they form highly regulated molecular machines that operate on membranes. Solution NMR is an ideal method to capture high-resolution glimpses of the molecular transformations that take place when these proteins come together and work on membranes...
April 2018: Progress in Nuclear Magnetic Resonance Spectroscopy
https://www.readbyqxmd.com/read/29544889/a-novel-stxbp1-mutation-causes-typical-rett-syndrome-in-a-japanese-girl
#12
Kotaro Yuge, Kazuhiro Iwama, Chihiro Yonee, Mayumi Matsufuji, Nozomi Sano, Tomoko Saikusa, Yukako Yae, Yushiro Yamashita, Takeshi Mizuguchi, Naomichi Matsumoto, Toyojiro Matsuishi
Rett syndrome (RTT) is a neurodevelopmental disorder mostly caused by mutations in Methyl-CpG-binding protein 2 (MECP2); however, mutations in various other genes may lead to RTT-like phenotypes. Here, we report the first case of a Japanese girl with RTT caused by a novel syntaxin-binding protein 1 (STXBP1) frameshift mutation (c.60delG, p.Lys21Argfs*16). She showed epilepsy at one year of age, regression of acquired psychomotor abilities thereafter, and exhibited stereotypic hand and limb movements at 3 years of age...
March 12, 2018: Brain & Development
https://www.readbyqxmd.com/read/29542271/ptdins-4-5-p-2-is-not-required-for-secretory-granule-docking
#13
Muhmmad Omar-Hmeadi, Nikhil Gandasi, Sebastian Barg
Phosphoinositides (PtdIns) play important roles in exocytosis and are thought to regulate secretory granule docking by co-clustering with the SNARE protein syntaxin to form a docking receptor in the plasma membrane. Here we tested this idea by high-resolution TIRF imaging of EGFP-labeled PtdIns markers or syntaxin-1 at secretory granule release sites in live insulin-secreting cells. In intact cells, PtdIns markers distributed evenly across the plasma membrane with no preference for granule docking sites. In contrast, syntaxin-1 was found clustered in the plasma membrane, mostly beneath docked granules...
March 14, 2018: Traffic
https://www.readbyqxmd.com/read/29511299/synaptic-loss-in-schizophrenia-a-meta-analysis-and-systematic-review-of-synaptic-protein-and-mrna-measures
#14
REVIEW
Emanuele Felice Osimo, Katherine Beck, Tiago Reis Marques, Oliver D Howes
Although synaptic loss is thought to be core to the pathophysiology of schizophrenia, the nature, consistency and magnitude of synaptic protein and mRNA changes has not been systematically appraised. Our objective was thus to systematically review and meta-analyse findings. The entire PubMed database was searched for studies from inception date to the 1st of July 2017. We selected case-control postmortem studies in schizophrenia quantifying synaptic protein or mRNA levels in brain tissue. The difference in protein and mRNA levels between cases and controls was extracted and meta-analysis conducted...
March 6, 2018: Molecular Psychiatry
https://www.readbyqxmd.com/read/29496544/frontotemporal-dysregulation-of-the-snare-protein-interactome-is-associated-with-faster-cognitive-decline-in-old-age
#15
Alfredo Ramos-Miguel, Andrea A Jones, Ken Sawada, Alasdair M Barr, Thomas A Bayer, Peter Falkai, Sue E Leurgans, Julie A Schneider, David A Bennett, William G Honer
The molecular underpinnings associated with cognitive reserve remain poorly understood. Because animal models fail to fully recapitulate the complexity of human brain aging, postmortem studies from well-designed cohorts are crucial to unmask mechanisms conferring cognitive resistance against cumulative neuropathologies. We tested the hypothesis that functionality of the SNARE protein interactome might be an important resilience factor preserving cognitive abilities in old age. Cognition was assessed annually in participants from the Rush "Memory and Aging Project" (MAP), a community-dwelling cohort representative of the overall aging population...
February 26, 2018: Neurobiology of Disease
https://www.readbyqxmd.com/read/29490270/spontaneous-vesicle-fusion-is-differentially-regulated-at-cholinergic-and-gabaergic-synapses
#16
Haowen Liu, Lei Li, Wei Wang, Jihong Gong, Xiaofei Yang, Zhitao Hu
The locomotion of C. elegans is balanced by excitatory and inhibitory neurotransmitter release at neuromuscular junctions. However, the molecular mechanisms that maintain the balance of synaptic transmission remain enigmatic. Here, we investigated the function of voltage-gated Ca2+ channels in triggering spontaneous release at cholinergic and GABAergic synapses. Recordings of the miniature excitatory/inhibitory postsynaptic currents (mEPSCs and mIPSCs, respectively) showed that UNC-2/CaV2 and EGL-19/CaV1 channels are the two major triggers for spontaneous release...
February 27, 2018: Cell Reports
https://www.readbyqxmd.com/read/29475951/soluble-syntaxin-3-functions-as-a-transcriptional-regulator
#17
Adrian J Giovannone, Christine Winterstein, Pallavi Bhattaram, Elena Reales, Seng Hui Low, Julie E Baggs, Mimi Xu, Matthew A Lalli, John B Hogenesch, Thomas Weimbs
Syntaxins are a conserved family of SNARE proteins and contain C-terminal transmembrane anchors required for their membrane fusion activity. Here we show that syntaxin 3 (Stx3) unexpectedly also functions as a nuclear regulator of gene expression. We found that alternative splicing creates a soluble isoform, we termed Stx3S, lacking the transmembrane anchor. Soluble Stx3S binds to the nuclear import factor RAN-binding protein 5 (RanBP5), targets to the nucleus and interacts physically and functionally with several transcription factors, including ETS variant 4 (ETV4) and activating transcription factor 2 (ATF2)...
February 23, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29449316/snareing-an-arp-requires-a-lir
#18
Sharon A Tooze
The fusion of autophagosomes with lysosomes is an obligatory step in the self-eating process of autophagy. In this issue, Kumar et al. (2018. J. Cell Biol. https://doi.org/10.1083/jcb.201708039) identify a protein complex, the autophagosome recognition particle (ARP), that chaperones a key SNARE, syntaxin 17, to the autophagosome membrane. Intriguingly, this protein complex coordinates both delivery and membrane insertion as a prelude to fusion.
February 15, 2018: Journal of Cell Biology
https://www.readbyqxmd.com/read/29445155/the-cytotoxicity-of-coxsackievirus-b3-is-associated-with-a-blockage-of-autophagic-flux-mediated-by-reduced-syntaxin-17-expression
#19
Lang Tian, Yeyi Yang, Chunyun Li, Jia Chen, Zhuoying Li, Xin Li, Shentang Li, Fang Wu, Zhangxue Hu, Zuocheng Yang
Coxsackievirus B3 (CVB3) is an important human pathogen linked to cardiac arrhythmias and acute heart failure. CVB3 infection has been reported to induce the formation of autophagosomes that support the viral replication in host cells. Interestingly, our study shows that the accumulation of autophagosomes during CVB3 infection is caused by a blockage of autophagosome-lysosome fusion rather than the induction of autophagosome biogenesis. Moreover, CVB3 decreases the transcription and translation of syntaxin 17 (STX17), a SNARE (soluble N-ethylmaleimide-sensitive factor activating protein receptor) protein involved in autophagosome-lysosome fusion...
February 14, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29426817/the-iap-family-member-bruce-regulates-autophagosome-lysosome-fusion
#20
Petra Ebner, Isabella Poetsch, Luiza Deszcz, Thomas Hoffmann, Johannes Zuber, Fumiyo Ikeda
Autophagy has an important role in cellular homeostasis by degrading and recycling cytotoxic components. Ubiquitination is known to target cargoes for autophagy; however, key components of this pathway remain elusive. Here we performed an RNAi screen to uncover ubiquitin modifiers that are required for starvation-induced macroautophagy in mammalian cells. Our screen uncovered BRUCE/Apollon/Birc6, an IAP protein, as a new autophagy regulator. Depletion of BRUCE leads to defective fusion of autophagosomes and lysosomes...
February 9, 2018: Nature Communications
keyword
keyword
12118
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"