keyword
MENU ▼
Read by QxMD icon Read
search

debose-boyd

keyword
https://www.readbyqxmd.com/read/27129778/contribution-of-accelerated-degradation-to-feedback-regulation-of-3-hydroxy-3-methylglutaryl-coenzyme-a-reductase-and-cholesterol-metabolism-in-the-liver
#1
Seonghwan Hwang, Isamu Z Hartman, Leona N Calhoun, Kristina Garland, Gennipher A Young, Matthew A Mitsche, Jeffrey McDonald, Fang Xu, Luke Engelking, Russell A DeBose-Boyd
Accumulation of sterols in endoplasmic reticulum membranes stimulates the ubiquitination of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), which catalyzes a rate-limiting step in synthesis of cholesterol. This ubiquitination marks HMGCR for proteasome-mediated degradation and constitutes one of several mechanisms for feedback control of cholesterol synthesis. Mechanisms for sterol-accelerated ubiquitination and degradation of HMGCR have been elucidated through the study of cultured mammalian cells...
June 24, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27121042/geranylgeranyl-regulated-transport-of-the-prenyltransferase-ubiad1-between-membranes-of-the-er-and-golgi
#2
Marc M Schumacher, Dong-Jae Jun, Youngah Jo, Joachim Seemann, Russell A DeBose-Boyd
UbiA prenyltransferase domain-containing protein-1 (UBIAD1) utilizes geranylgeranyl pyrophosphate (GGpp) to synthesize the vitamin K2 subtype menaquinone-4. Previously, we found that sterols trigger binding of UBIAD1 to endoplasmic reticulum (ER)-localized HMG-CoA reductase, the rate-limiting enzyme in synthesis of cholesterol and nonsterol isoprenoids, including GGpp. This binding inhibits sterol-accelerated degradation of reductase, which contributes to feedback regulation of the enzyme. The addition to cells of geranylgeraniol (GGOH), which can become converted to GGpp, triggers release of UBIAD1 from reductase, allowing for its maximal degradation and permitting ER-to-Golgi transport of UBIAD1...
July 2016: Journal of Lipid Research
https://www.readbyqxmd.com/read/26712526/transcription-factor-hepatocyte-nuclear-factor-1%C3%AE-regulates-renal-cholesterol-metabolism
#3
Karam Aboudehen, Min Soo Kim, Matthew Mitsche, Kristina Garland, Norma Anderson, Lama Noureddine, Marco Pontoglio, Vishal Patel, Yang Xie, Russell DeBose-Boyd, Peter Igarashi
HNF-1β is a tissue-specific transcription factor that is expressed in the kidney and other epithelial organs. Humans with mutations in HNF-1β develop kidney cysts, and HNF-1β regulates the transcription of several cystic disease genes. However, the complete spectrum of HNF-1β-regulated genes and pathways is not known. Here, using chromatin immunoprecipitation/next generation sequencing and gene expression profiling, we identified 1545 protein-coding genes that are directly regulated by HNF-1β in murine kidney epithelial cells...
August 2016: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/25742604/the-prenyltransferase-ubiad1-is-the-target-of-geranylgeraniol-in-degradation-of-hmg-coa-reductase
#4
Marc M Schumacher, Rania Elsabrouty, Joachim Seemann, Youngah Jo, Russell A DeBose-Boyd
Schnyder corneal dystrophy (SCD) is an autosomal dominant disorder in humans characterized by abnormal accumulation of cholesterol in the cornea. SCD-associated mutations have been identified in the gene encoding UBIAD1, a prenyltransferase that synthesizes vitamin K2. Here, we show that sterols stimulate binding of UBIAD1 to the cholesterol biosynthetic enzyme HMG CoA reductase, which is subject to sterol-accelerated, endoplasmic reticulum (ER)-associated degradation augmented by the nonsterol isoprenoid geranylgeraniol through an unknown mechanism...
2015: ELife
https://www.readbyqxmd.com/read/24860107/sequential-actions-of-the-aaa-atpase-valosin-containing-protein-vcp-p97-and-the-proteasome-19-s-regulatory-particle-in-sterol-accelerated-endoplasmic-reticulum-er-associated-degradation-of-3-hydroxy-3-methylglutaryl-coenzyme-a-reductase
#5
Lindsey L Morris, Isamu Z Hartman, Dong-Jae Jun, Joachim Seemann, Russell A DeBose-Boyd
Accelerated endoplasmic reticulum (ER)-associated degradation (ERAD) of the cholesterol biosynthetic enzyme 3-hydroxy-3-methylglutaryl-coenzyme A reductase results from its sterol-induced binding to ER membrane proteins called Insig-1 and Insig-2. This binding allows for subsequent ubiquitination of reductase by Insig-associated ubiquitin ligases. Once ubiquitinated, reductase becomes dislocated from ER membranes into the cytosol for degradation by 26 S proteasomes through poorly defined reactions mediated by the AAA-ATPase valosin-containing protein (VCP)/p97 and augmented by the nonsterol isoprenoid geranylgeraniol...
July 4, 2014: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/24062519/sufficient-production-of-geranylgeraniol-is-required-to-maintain-endotoxin-tolerance-in-macrophages
#6
Jinyong Kim, Joon No Lee, James Ye, Rosy Hao, Russell Debose-Boyd, Jin Ye
Endotoxin tolerance allows macrophages to produce large quantities of proinflammatory cytokines immediately after their contact with lipopolysaccharides (LPSs), but prevents their further production after repeated exposure to LPSs. While this response is known to prevent overproduction of proinflammatory cytokines, the mechanism through which endotoxin tolerance is established has not been identified. In the current study, we demonstrate that sufficient production of geranylgeraniol (GGOH) in macrophages is required to maintain endotoxin tolerance...
December 2013: Journal of Lipid Research
https://www.readbyqxmd.com/read/24025715/sterol-induced-dislocation-of-3-hydroxy-3-methylglutaryl-coenzyme-a-reductase-from-membranes-of-permeabilized-cells
#7
Rania Elsabrouty, Youngah Jo, Tammy T Dinh, Russell A DeBose-Boyd
The polytopic endoplasmic reticulum (ER)-localized enzyme 3-hydroxy-3-methylglutaryl CoA reductase catalyzes a rate-limiting step in the synthesis of cholesterol and nonsterol isoprenoids. Excess sterols cause the reductase to bind to ER membrane proteins called Insig-1 and Insig-2, which are carriers for the ubiquitin ligases gp78 and Trc8. The resulting gp78/Trc8-mediated ubiquitination of reductase marks it for recognition by VCP/p97, an ATPase that mediates subsequent dislocation of reductase from ER membranes into the cytosol for proteasomal degradation...
November 2013: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/24006306/geranylgeraniol-suppresses-the-viability-of-human-du145-prostate-carcinoma-cells-and-the-level-of-hmg-coa-reductase
#8
Nicolle V Fernandes, Hoda Yeganehjoo, Rajasekhar Katuru, Russell A DeBose-Boyd, Lindsey L Morris, Renee Michon, Zhi-Ling Yu, Huanbiao Mo
The rate-limiting enzyme of the mevalonate pathway, 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, provides essential intermediates for the prenylation of nuclear lamins and Ras and dolichol-mediated glycosylation of growth factor receptors. The diterpene geranylgeraniol downregulates the level of HMG CoA reductase and suppresses the growth of human liver, lung, ovary, pancreas, colon, stomach, and blood tumors. We evaluated the growth-suppressive activity of geranylgeraniol in human prostate carcinoma cells...
November 1, 2013: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/23580362/opening-up-new-fronts-in-the-fight-against-cholesterol
#9
COMMENT
Russell A Debose-Boyd, Jay D Horton
An unexpected connection between a secretory protein called PCSK9 and Sec24A, a well known protein-transport protein, could lead to the development of novel treatments for patients with high levels of low-density lipoproteins in their blood.
April 9, 2013: ELife
https://www.readbyqxmd.com/read/23403031/lipid-regulated-degradation-of-hmg-coa-reductase-and-insig-1-through-distinct-mechanisms-in-insect-cells
#10
Rebecca A Faulkner, Andrew D Nguyen, Youngah Jo, Russell A DeBose-Boyd
In mammalian cells, levels of the integral membrane proteins 3-hydroxy-3-methylglutaryl-CoA reductase and Insig-1 are controlled by lipid-regulated endoplasmic reticulum-associated degradation (ERAD). The ERAD of reductase slows a rate-limiting step in cholesterol synthesis and results from sterol-induced binding of its membrane domain to Insig-1 and the highly related Insig-2 protein. Insig binding bridges reductase to ubiquitin ligases that facilitate its ubiquitination, thereby marking the protein for cytosolic dislocation and proteasomal degradation...
April 2013: Journal of Lipid Research
https://www.readbyqxmd.com/read/23223569/ancient-ubiquitous-protein-1-mediates-sterol-induced-ubiquitination-of-3-hydroxy-3-methylglutaryl-coa-reductase-in-lipid-droplet-associated-endoplasmic-reticulum-membranes
#11
Youngah Jo, Isamu Z Hartman, Russell A DeBose-Boyd
Sterol-induced binding to Insigs in endoplasmic reticulum (ER) membranes triggers ubiquitination of the cholesterol biosynthetic enzyme 3-hydroxy-3-methylglutaryl CoA reductase. This ubiquitination, which is mediated by Insig-associated ubiquitin ligases gp78 and Trc8, is obligatory for extraction of reductase from lipid droplet-associated ER membranes into the cytosol for proteasome-mediated, ER-associated degradation (ERAD). In this study, we identify lipid droplet-associated, ancient, ubiquitous protein-1 (Aup1) as one of several proteins that copurify with gp78...
February 2013: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/22143767/sterol-induced-degradation-of-hmg-coa-reductase-depends-on-interplay-of-two-insigs-and-two-ubiquitin-ligases-gp78-and-trc8
#12
Youngah Jo, Peter C W Lee, Peter V Sguigna, Russell A DeBose-Boyd
Accumulation of sterols in membranes of the endoplasmic reticulum (ER) leads to the accelerated ubiquitination and proteasomal degradation of 3-hydroxy-3-methylglutaryl coenzyme A reductase, a rate-limiting enzyme in synthesis of cholesterol and nonsterol isoprenoids. This degradation results from sterol-induced binding of reductase to the Insig-1 or Insig-2 proteins of ER membranes. We previously reported that in immortalized human fibroblasts (SV-589 cells) Insig-1, but not Insig-2, recruits gp78, a membrane-bound RING-finger ubiquitin ligase...
December 20, 2011: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/21504873/regulation-of-cholesterol-and-fatty-acid-synthesis
#13
REVIEW
Jin Ye, Russell A DeBose-Boyd
In mammals, intracellular levels of cholesterol and fatty acids are controlled through a feedback regulatory system mediated by a family of transcription factors called sterol regulatory element-binding proteins (SREBPs). SREBPs are synthesized as inactive precursors bound to membranes of the endoplasmic reticulum. When cells are deprived of cholesterol and fatty acids, NH(2)-terminal fragments of SREBPs become proteolytically released from membranes and migrate to the nucleus to activate transcription of genes required for lipid synthesis and uptake...
July 2011: Cold Spring Harbor Perspectives in Biology
https://www.readbyqxmd.com/read/21343306/membrane-associated-ubiquitin-ligase-complex-containing-gp78-mediates-sterol-accelerated-degradation-of-3-hydroxy-3-methylglutaryl-coenzyme-a-reductase
#14
Youngah Jo, Peter V Sguigna, Russell A DeBose-Boyd
The endoplasmic reticulum (ER)-associated degradation (ERAD) pathway in the yeast Saccharomyces cerevisiae is mediated by two membrane-bound ubiquitin ligases, Doa10 and Hrd1. These enzymes are found in distinct multiprotein complexes that allow them to recognize and target a variety of substrates for proteasomal degradation. Although multiprotein complexes containing mammalian ERAD ubiquitin ligases likely exist, they have yet to be identified and characterized in detail. Here, we identify two ER membrane proteins, SPFH2 and TMUB1, as associated proteins of mammalian gp78, a membrane-bound ubiquitin ligase that bears significant sequence homology with mammalian Hrd1 and mediates sterol-accelerated ERAD of the cholesterol biosynthetic enzyme HMG-CoA reductase...
April 29, 2011: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/21217781/uncoupling-hypoxia-signaling-from-oxygen-sensing-in-the-liver-results-in-hypoketotic-hypoglycemic-death
#15
B Kucejova, N E Sunny, A D Nguyen, R Hallac, X Fu, S Peña-Llopis, R P Mason, R J Deberardinis, X-J Xie, R Debose-Boyd, V D Kodibagkar, S C Burgess, J Brugarolas
As the ultimate electron acceptor in oxidative phosphorylation, oxygen plays a critical role in metabolism. When oxygen levels drop, heterodimeric hypoxia-inducible factor (Hif) transcription factors become active and facilitate adaptation to hypoxia. Hif regulation by oxygen requires the protein von Hippel-Lindau (pVhl) and pVhl disruption results in constitutive Hif activation. The liver is a critical organ for metabolic homeostasis, and Vhl inactivation in hepatocytes results in a Hif-dependent shortening in life span...
May 5, 2011: Oncogene
https://www.readbyqxmd.com/read/20482385/control-of-cholesterol-synthesis-through-regulated-er-associated-degradation-of-hmg-coa-reductase
#16
REVIEW
Youngah Jo, Russell A Debose-Boyd
Multiple mechanisms for feedback control of cholesterol synthesis converge on the rate-limiting enzyme in the pathway, 3-hydroxy-3-methylglutaryl coenzyme A reductase. This complex feedback regulatory system is mediated by sterol and nonsterol metabolites of mevalonate, the immediate product of reductase activity. One mechanism for feedback control of reductase involves rapid degradation of the enzyme from membranes of the endoplasmic reticulum (ER). This degradation results from the accumulation of sterols in ER membranes, which triggers binding of reductase to ER membrane proteins called Insig-1 and Insig-2...
June 2010: Critical Reviews in Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/20406816/sterol-induced-dislocation-of-3-hydroxy-3-methylglutaryl-coenzyme-a-reductase-from-endoplasmic-reticulum-membranes-into-the-cytosol-through-a-subcellular-compartment-resembling-lipid-droplets
#17
Isamu Z Hartman, Pingsheng Liu, John K Zehmer, Katherine Luby-Phelps, Youngah Jo, Richard G W Anderson, Russell A DeBose-Boyd
Sterol-induced binding to Insigs in the endoplasmic reticulum (ER) allows for ubiquitination of 3-hydroxy-3-methylglutaryl coenzyme A reductase, the rate-limiting enzyme in cholesterol synthesis. This ubiquitination marks reductase for recognition by the ATPase VCP/p97, which mediates extraction and delivery of reductase from ER membranes to cytosolic 26 S proteasomes for degradation. Here, we report that reductase becomes dislocated from ER membranes into the cytosol of sterol-treated cells. This dislocation exhibits an absolute requirement for the actions of Insigs and VCP/p97...
June 18, 2010: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/19638338/insig-mediated-sterol-accelerated-degradation-of-the-membrane-domain-of-hamster-3-hydroxy-3-methylglutaryl-coenzyme-a-reductase-in-insect-cells
#18
Andrew D Nguyen, Soo Hee Lee, Russell A DeBose-Boyd
Sterol-accelerated degradation of the enzyme 3-hydroxy-3-methylglutaryl-coenzyme A reductase is one of several mechanisms through which cholesterol synthesis is controlled in mammalian cells. This degradation results from sterol-induced binding of the membrane domain of reductase to endoplasmic reticulum membrane proteins called Insig-1 and Insig-2, which are carriers of a ubiquitin ligase called gp78. The ensuing gp78-mediated ubiquitination of reductase is a prerequisite for its rapid, 26 S proteasome-mediated degradation from endoplasmic reticulum membranes, a reaction that slows a rate-limiting step in cholesterol synthesis...
September 25, 2009: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/19617589/intramembrane-glycine-mediates-multimerization-of-insig-2-a-requirement-for-sterol-regulation-in-chinese-hamster-ovary-cells
#19
Peter C W Lee, Russell A DeBose-Boyd
Sterol-induced binding of endoplasmic reticulum (ER) membrane proteins Insig-1 and Insig-2 to SREBP cleavage-activating protein (Scap) and HMG-CoA reductase triggers regulatory events that limit cholesterol synthesis in animal cells. Binding of Insigs to Scap prevents proteolytic activation of sterol-regulatory element binding proteins (SREBPs), membrane-bound transcription factors that enhance cholesterol synthesis, by trapping Scap-SREBP complexes in the ER. Insig binding to reductase causes ubiquitination and subsequent proteasome-mediated degradation of the enzyme from ER membranes, slowing a rate-limiting step in cholesterol synthesis...
January 2010: Journal of Lipid Research
https://www.readbyqxmd.com/read/18504457/feedback-regulation-of-cholesterol-synthesis-sterol-accelerated-ubiquitination-and-degradation-of-hmg-coa-reductase
#20
REVIEW
Russell A DeBose-Boyd
3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase produces mevalonate, an important intermediate in the synthesis of cholesterol and essential nonsterol isoprenoids. The reductase is subject to an exorbitant amount of feedback control through multiple mechanisms that are mediated by sterol and nonsterol end-products of mevalonate metabolism. Here, I will discuss recent advances that shed light on one mechanism for control of reductase, which involves rapid degradation of the enzyme. Accumulation of certain sterols triggers binding of reductase to endoplasmic reticulum (ER) membrane proteins called Insig-1 and Insig-2...
June 2008: Cell Research
keyword
keyword
121173
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"