Christos Papantoniou, Ulrike Laugks, Julia Betzin, Cristina Capitanio, José Javier Ferrero, José Sánchez-Prieto, Susanne Schoch, Nils Brose, Wolfgang Baumeister, Benjamin H Cooper, Cordelia Imig, Vladan Lučić
Synaptic vesicle tethering, priming, and neurotransmitter release require a coordinated action of multiple protein complexes. While physiological experiments, interaction data, and structural studies of purified systems were essential for our understanding of the function of the individual complexes involved, they cannot resolve how the actions of individual complexes integrate. We used cryo-electron tomography to simultaneously image multiple presynaptic protein complexes and lipids at molecular resolution in their native composition, conformation, and environment...
June 23, 2023: Science Advances