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Liu Lu, Yu Feng, Haiyan Long, Alan McNally, Zhiyong Zong
A carbapenem-resistant Klebsiella pneumoniae was recovered from human blood. Its whole genome sequence was obtained using both Illumina and long-read MinION sequencing. The strain belongs to ST273, which has been found recently and caused an outbreak in Southeast Asia. It has two carbapenemase genes bla NDM-1 (carried by an ST7 IncN self-transmissible plasmid) and bla IMP-4 (located on an self-transmissible IncHI5 plasmid). Non-KPC-producing ST237 may represent a lineage of carbapenem-resistant K. pneumoniae , which warrants further monitoring...
April 2, 2018: Antimicrobial Agents and Chemotherapy
Xingyu Zhang, Di Chen, Guifeng Xu, Weichun Huang, Xing Wang
Infection by carbapenem-resistant Klebsiella pneumoniae (CR-KP) is a public health challenge worldwide, in particular among children, which was associated with high morbidity and mortality rates. There was limited data in pediatric populations, thus this study aimed to investigate molecular epidemiology and drug resistant mechanism of CR-KP strains from pediatric patients in Shanghai, China. A total of 41 clinical CR-KP isolates from sputum, urine, blood or drainage fluid were collected between July 2014 and May 2015 in Shanghai Children's Medical Center...
2018: PloS One
Li Fan, Zhe Wang, Qiang Wang, Zhijian Xiong, Ying Xu, Dandan Li, Shiwen Zhou
Objective: Acinetobacter baumannii is an opportunistic pathogen found in immunocompromised patients, especially cancer patients. This study was to investigate the clinical characteristics of cancer patients and the antimicrobial resistance of A.baumannii isolates. Materials and Methods: Clinical isolates were collected from the oncology department of a general teaching hospital, and the clinical and demographic information of patients was obtained from the hospital's information system...
January 2018: Journal of Cancer Research and Therapeutics
Monika Dolejska, Costas C Papagiannitsis, Matej Medvecky, Lenka Davidova-Gerzova, Adam Valcek
The complete nucleotide sequences of six IMP-4-encoding plasmids recovered from Enterobacteriaceae isolates of wildlife origin were characterized. Sequencing data showed that plasmids of different incompatibility groups (IncM, IncI1, IncF, and nontypeable [including an IncX5_2 and two pPrY2001-like]) carried the bla IMP-4 -carrying integrons In809 or In1460. Most of the plasmids carried an mph (A) region, and chrA -like, aac(3)-IId , and bla TEM-1b genes. Finally, plasmid analysis revealed the involvement of two different IS 26 - and Tn 1696 -associated mechanisms in the mobilization of IMP-4-encoding integrons...
May 2018: Antimicrobial Agents and Chemotherapy
Kai Zhou, Wei Yu, Ping Shen, Haifeng Lu, Baohong Wang, John W A Rossen, Yonghong Xiao
Genetic determinants of a clinical Klebsiella pneumoniae isolate (KP1814) coproducing IMP-4 and a rare ESBL gene SFO-1 was investigated. KP1814 belongs to a novel sequence type (ST) assigned to ST2270. WGS identified four circular DNA sequences in KP1814, including two multidrug-resistance (MDR) plasmids, one virulence plasmid, and one circular form. The MDR plasmid pKP1814-1 (299.9 Kb) is untypeable, and carries two large mosaic multiresistance regions (MRRs). blaSFO-1 and blaIMP-4 co-exists on MRR1, and blaSFO-1 is associated with an IS/Tn-independent genetic context...
December 11, 2017: Scientific Reports
Fang Dong, Jie Lu, Yan Wang, Jin Shi, Jing Hui Zhen, Ping Chu, Yang Zhen, Shu Jing Han, Yong Li Guo, Wen Qi Song
OBJECTIVE: To characterize carbapenem (CPM)-non-susceptible Klebsiella pneumoniae (K. pneumoniae) and carbape-nemase produced by these strains isolated from Beijing Children's Hospital based on a five-year surveillance. METHODS: The Minimal Inhibition Concentration values for 15 antibiotics were assessed using the Phonix100 compact system. PCR amplification and DNA sequencing were used to detect genes encoding carbapenemases. WHONET 5.6 was finally used for resistance analysis...
August 2017: Biomedical and Environmental Sciences: BES
Yan Yang, Jian Chen, Di Lin, Xujian Xu, Jun Cheng, Changgui Sun
With the abuse of antimicrobial agents in developing countries, increasing number of carbapenem-resistant Enterobacteriaceae (CRE) attracted considerable public concern. A retrospective study was conducted based on 242 CRE strains from a tertiary hospital in Hangzhou, China to investigate prevalence and drug resistance characteristics of CRE in southeast China. Bacterial species were identified. Antimicrobial susceptibility was examined by broth microdilution method or epsilometer test. Resistant β-lactamase genes were identified by polymerase chain reaction and sequencing...
July 8, 2017: Frontiers of Medicine
Juan Wang, Min Yuan, Hai Chen, Xia Chen, Yuanchun Jia, Xiong Zhu, Li Bai, Xue Bai, Séamus Fanning, Jinxing Lu, Juan Li
The nucleotide sequences of five plasmids from one Klebsiella oxytoca isolate were determined using the PacBio RS II system. Plasmid analysis revealed that bla NDM-1 was carried on an IncX3 plasmid. The bla IMP-4 and bla KPC-2 genes were located on IncN and IncP-6 plasmids, respectively. Comparative sequence analysis highlighted the successful spread of carbapenemase-harboring plasmids among different enterobacterial species. We report for the first time, to our knowledge, coproducing NDM-1, KPC-2, and IMP-4 carbapenemases on a K...
September 2017: Antimicrobial Agents and Chemotherapy
Kaisheng Lai, Yanning Ma, Ling Guo, Jingna An, Liyan Ye, Jiyong Yang
BACKGROUND: IMP-producing Klebsiella pneumoniae (IMPKpn) exhibits sporadic prevalence in China. The mechanisms related to the spread of IMPKpn remain unclear. METHODS: Carbapenem non-susceptible K. pneumoniae isolates were collected from our hospital. The genetic relatedness, antimicrobial susceptibility, as well as sequence types (ST) were analyzed by pulsed-field gel electrophoresis (PFGE), VITEK 2 AST test Kit, and multilocus sequence typing (MLST), respectively...
June 19, 2017: Annals of Clinical Microbiology and Antimicrobials
Arpasiri Srisrattakarn, Aroonlug Lulitanond, Chotechana Wilailuckana, Nicha Charoensri, Lumyai Wonglakorn, Pimjai Saenjamla, Prajuab Chaimanee, Jureerut Daduang, Aroonwadee Chanawong
Carbapenem-resistant Enterobacteriaceae isolates by carbapenemase production are being reported globally with increasing frequency, leading to limited therapeutic options. We therefore developed a loop-mediated isothermal amplification method with hydroxynaphthol blue dye (LAMP-HNB) for rapid confirmation of bla NDM , bla OXA-48 , bla VIM , bla IMP-14 and bla KPC groups. Sixty-two Enterobacteriaceae and Pseudomonas spp. isolates carrying various carbapenemase genes (28 bla NDM-1 , 9 bla IMP-14a , 2 bla IMP-48 , 1 bla IMP-1 , 1 bla IMP-4 , 1 bla IMP-9 , 1 bla IMP-15 , 4 bla VIM-2 , 1 bla VIM-1 , 1 bla IMP-14a & bla VIM-2 , 7 bla KPC-2 , 3 bla OXA-48 and 3 bla OXA-181 ) and 37 non-carbapenemase-producing Enterobacteriaceae isolates as confirmed by the PCR methods were included...
July 2017: World Journal of Microbiology & Biotechnology
Wenbo Lin, PengPeng Xu, YingYing Guo, Qingjie Jia, Tao Tao
Nkx2-2 homeoprotein is essential for the development of the central nervous system and pancreas. Although the nuclear localization signals of Nkx2-2 have been identified, the responsible transport receptor is still unknown. Here, we demonstrate that imp α1 not only interacts with Nkx2-2 but also transports it into the nucleus in vitro by acting together with imp β1. However, the nuclear import of Nkx2-2 in cells was not inhibited in response to knockdown expression of endogenous imp β1 or over-expression of Bimax2...
January 22, 2017: Biochemical and Biophysical Research Communications
Sam Abraham, Mark O'Dea, Darren J Trott, Rebecca J Abraham, David Hughes, Stanley Pang, Genevieve McKew, Elaine Y L Cheong, John Merlino, Sugiyono Saputra, Richard Malik, Thomas Gottlieb
Carbapenem-resistant Enterobacteriaceae (CRE) are a pressing public health issue due to limited therapeutic options to treat such infections. CREs have been predominantly isolated from humans and environmental samples and they are rarely reported among companion animals. In this study we report on the isolation and plasmid characterization of carbapenemase (IMP-4) producing Salmonella enterica Typhimurium from a companion animal. Carbapenemase-producing S. enterica Typhimurium carrying blaIMP-4 was identified from a systemically unwell (index) cat and three additional cats at an animal shelter...
October 21, 2016: Scientific Reports
Wei Feng, Dongsheng Zhou, Qian Wang, Wenbo Luo, Defu Zhang, Qiang Sun, Yigang Tong, Weijun Chen, Fengjun Sun, Peiyuan Xia
A total of 26 blaIMP-4-carrying strains of Pseudomonas aeruginosa and Klebsiella pneumoniae were isolated from 2009 to 2013 in a Chinese teaching hospital, and these strains can be assigned into multiple sequence types or allelic profiles as determined by multilocus sequence typing. Of these strains, P. aeruginosa P378 and K. pneumoniae 1220 harbor the IMP-4-encoding plasmids pP378-IMP and p1220-IMP, respectively, whose complete nucleotide sequences are determined to be genetically closely related to the IncN1-type plasmid pIMP-HZ1...
September 19, 2016: Scientific Reports
Q Wang, Y Zhang, X Yao, H Xian, Y Liu, H Li, H Chen, X Wang, R Wang, C Zhao, B Cao, H Wang
This study was aimed to determine the risk factors of Carbapenem-resistant Enterobacteriaceae (CRE) nosocomial infections and assess the clinical outcomes. A case-case-control design was used to compare two groups of case patients with control patients from March 2010 to November 2014 in China. Risk factors for the acquisition of CRE infections and clinical outcomes were analyzed by univariable and multivariable analysis. A total of 94 patients with CRE infections, 93 patients with Carbapenem-susceptible Enterobacteriaceae (CSE) infections, and 93 patients with organisms other than Enterobacteriaceae infections were enrolled in this study...
October 2016: European Journal of Clinical Microbiology & Infectious Diseases
Namraj Goire, Gerald B Harnett, Lyn C O'Reilly, Paul R Ingram, Michael J Leung, David J Speers, Paul E Healy, Timothy J J Inglis
No abstract text is available yet for this article.
July 5, 2016: Journal of Microbiological Methods
Feng Pang, Xiu-Qin Jia, Zhen-Zhu Song, Yan-Hua Li, Bin Wang, Qi-Gang Zhao, Chuan-Xin Wang, Yi Zhang, Le-Xin Wang
BACKGROUND: The emergence of Enterobacteriaceae harboring IMP-4 or IMP-8 carbapenemases is rare. We report an occurrence of Enterobacteriaceae harboring IMP-4 or IMP-8 carbapenemases in a Chinese tertiary care hospital from November 2010 to December 2012. METHODS: The clinical characteristics of 30 patients were described. The genetic relationship of isolates was determined by pulsed-field gel electrophoresis (PFGE). Carbapenemases were detected by modified Hodge test (MHT) and polymerase chain reactions (PCRs)...
March 2016: African Health Sciences
Arpasiri Srisrattakarn, Aroonlug Lulitanond, Chotechana Wilailuckana, Nicha Charoensri, Lumyai Wonglakorn, Sirikan Piyapatthanakul, Ampai Supajeen, Aroonwadee Chanawong
Carbapenemase-producing Enterobacteriaceae (CPE) isolates have now emerged worldwide. We therefore modified the phenotypic Carba NP test by use of a filter paper strip for easily and rapidly identifying CPE in routine laboratory. A collection of 56 CPE and carbapenemase-producing Pseudomonas spp. isolates (including 28 NDM-1, 11 IMP-14a, 1 IMP-1, 1 IMP-4, 1 IMP-9, 1 IMP-15, 4 VIM-2, 1 VIM-1, 1 IMP-14a with VIM-2, 3 OXA-48, 3 OXA-181 and 1 KPC-2 producers) and 41 non-CPE isolates (including 19 ESBL, 7 pAmpC, 3 AmpC, 9 ESBL with pAmpC and 3 non-ESBL & non-AmpC producers) as confirmed by the PCR methods were tested by the paper strip method using pharmaceutical imipenem/cilastatin as a substrate...
July 2016: World Journal of Microbiology & Biotechnology
Jun Li, Mingxiang Zou, Qingya Dou, Yongmei Hu, Haichen Wang, Qun Yan, Wen' En Liu
BACKGROUND: Pseudomonas aeruginosa strains that are classed as extensively drug resistant (XDR-PA) are resistant to all antibiotics except for one or two classes and are frequently the cause of hard-to-treat infections worldwide. Our study aimed to characterize clinical XDR-PA isolates recovered during 2011-2012 at nine hospitals in the Hunan province of China. METHODS: Thirty-seven non-repetitive XDR-PA strains from 37 patients were investigated for genes encoding antimicrobial resistance determinants, efflux pumps, outer membrane proteins, and movable genetic elements using polymerase chain reaction (PCR)...
May 23, 2016: Annals of Clinical Microbiology and Antimicrobials
Xiao-Li Cao, Li Cheng, Zhi-Feng Zhang, Ming-Zhe Ning, Wan-Qing Zhou, Kui Zhang, Han Shen
A total of 72 consecutive and nonduplicate clinical extended-spectrum β-lactamase (ESBL)-producing Enterobacter cloacae isolates were collected from our hospital from 2012 to 2014 for analyzing the prevalence of plasmid-mediated quinolone resistance (PMQR) genes, 16S rRNA methyltransferase (16S-RMTase) encoding genes, and carbapenem-hydrolyzing β-lactamase (CHβL) genes, as well as integrons. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were carried out to characterize the genetic relatedness...
January 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
Namraj Goire, Gerald B Harnett, Lyn C O'Reilly, Paul R Ingram, Michael J Leung, Davild J Speers, Paul E Healy, Timothy J J Inglis
A local predominance of carbapenemase producing Enterobacteriaceae with low minimum inhibitory concentrations (MIC) to meropenem prompted a review of methods available for carbapenemase detection. We report on results using two selective media, temocillin discs, CarbaNP test, GeneXpert Carba-R assay and an in-house PCR assay.
May 2016: Journal of Microbiological Methods
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