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https://www.readbyqxmd.com/read/29776711/preliminary-assessment-of-the-diagnostic-performances-of-a-new-rapid-diagnostic-test-for-the-serodiagnosis-of-human-cystic-echinococcosis
#1
REVIEW
Ambra Vola, Francesca Tamarozzi, Rahmah Noordin, Muhammad Hafiznur Yunus, Sam Khanbabaie, Annalisa De Silvestri, Enrico Brunetti, Mara Mariconti
Rapid diagnostic tests for cystic echinococcosis (CE) are convenient to support ultrasound diagnosis in uncertain cases, especially in resource-limited settings. We found comparable diagnostic performances of the experimental Hyd Rapid Test and the commercial VIRapid HYDATIDOSIS Test, used in our diagnostic laboratory, using samples from well-characterized hepatic CE cases.
April 14, 2018: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29774832/intense-focus-of-alveolar-echinococcosis-south-kyrgyzstan
#2
Bakhadyr Bebezov, Nurlan Mamashev, Tilek Umetaliev, Iskender Ziadinov, Philip S Craig, Deborah E Joekel, Peter Deplazes, Felix Grimm, Paul R Torgerson
Human alveolar echinococcosis (AE) is a highly pathogenic zoonotic parasitic disease caused by Echinococcus multilocularis. An ultrasound study in southern Kyrgyzstan during 2012 revealed a prevalence of 4.2% probable or confirmed AE and an additional 2.2% possible AE, representing an emerging situation. The risk for probable or confirmed AE was significantly higher in dog owners.
June 2018: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/29774298/cystic-echinococcosis-in-marketed-offal-of-sheep-in-basrah-iraq-abattoir-based-survey-and-a-probabilistic-model-estimation-of-the-direct-economic-losses-due-to-hydatid-cyst
#3
Mohanad F Abdulhameed, Ihab Habib, Suzan A Al-Azizz, Ian Robertson
Cystic echinococcosis (CE) is a highly endemic parasitic zoonosis in Iraq with substantial impacts on livestock productivity and human health. The objectives of this study were to study the abattoir-based occurrence of CE in marketed offal of sheep in Basrah province, Iraq, and to estimate, using a probabilistic modelling approach, the direct economic losses due to hydatid cysts. Based on detailed visual meat inspection, results from an active abattoir survey in this study revealed detection of hydatid cysts in 7...
February 2018: Parasite Epidemiology and Control
https://www.readbyqxmd.com/read/29773136/molecular-characterization-of-echinococcus-species-in-dogs-from-four-regions-of-kenya
#4
Erastus Mulinge, Japhet Magambo, David Odongo, Sammy Njenga, Eberhard Zeyhle, Cecilia Mbae, Dorothy Kagendo, Francis Addy, Dennis Ebi, Marion Wassermann, Peter Kern, Thomas Romig
Cystic echinococcosis is endemic both in livestock and humans in many parts of Kenya. However, very little data exists on Echinococcus infections in dogs, and therefore their role in maintaining the transmission cycles and environmental contamination with eggs of Echinococcus species is unknown. The study aimed to establish the prevalence and distribution of Echinococcus granulosus sensu lato causing infection in dogs in Kenya. A total of 1621 dog faecal samples were collected from the environment in four different regions and examined microscopically for the presence of taeniid eggs...
May 15, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29771992/first-description-of-echinococcus-ortleppi-and-cystic-echinococcosis-infection-status-in-chile
#5
Felipe Corrêa, Caroll Stoore, Pamina Horlacher, Mauricio Jiménez, Christian Hidalgo, Cristian A Alvarez Rojas, Guilherme Figueiredo Barros, Henrique Bunselmeyer Ferreira, Marcela Hernández, Gonzalo Cabrera, Rodolfo Paredes
Cystic echinococcosis (CE), a parasitic disease caused by the cestode Echinococcus granulosus sensu lato (s.l.), is a worldwide zoonotic infection. Although endemic in Chile, information on the molecular characteristics of CE in livestock remains scarce. Therefore we aimed to describe the status of infection with E. granulosus s.l. in cattle from central Chile and also to contribute to the study of the molecular epidemiology of this parasite. According to our results, the prevalence of CE is 18.84% in cattle, similar to previous reports from Chile, suggesting that the prevalence in Santiago Metropolitan area has not changed in the last 30 years...
2018: PloS One
https://www.readbyqxmd.com/read/29770659/-investigation-on-unhealthy-related-behaviors-of-echinococcosis-patients-in-garz%C3%A3%C2%AA-prefecture-sichuan-province
#6
Tian Ke-Qing, Tian Tian, Shi Guo-Qing, Cao Chun-Li, Hao Yu-Wan, Zhu Ze-Lin, Zhou Xiao-Nong
OBJECTIVE: To understand the unhealthy related behaviors of echinococcosis patients in Tibetan region in Sichuan Province, so as to provide the references for the health education of echinococcosis. METHODS: The echinococcosis patients who were registered in the Tibetan region in Sichuan Province were selected as target population. The basic situation and unhealthy related behaviors of them were surveyed by individual interviews. RESULTS: Totally 368 echinococcosis patients were investigated...
April 17, 2018: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
https://www.readbyqxmd.com/read/29770658/-a-case-control-study-on-risk-factors-of-cystic-echinococcosis-in-humans-in-tibetan-areas
#7
Wu Wen-Ting, Wu Wei-Ping, Guan Ya-Yi, Han Shuai, Xue Chui-Zhao, Wang Xu, Liu Bai-Xue
OBJECTIVE: To understand the risk factors of cystic echinococcosis in Tibetan population, so as to provide the reference for development of prevention and control strategies. METHODS: Four cystic echinococcosis highly-prevalent townships were selected from Naqu County of Tibet and Shiqu County of Sichuan Province, which had similar environmental and altitude conditions, from August to September, 2017. Applying the 1∶1 case-control study, a household survey was conducted to investigate the risk factors of echinococcosis in humans...
April 16, 2018: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
https://www.readbyqxmd.com/read/29770657/-cloning-expression-and-immunity-analysis-of-transketolase-of-echinococcus-granulosus
#8
Cao De-Ping, Zhang Yao-Gang, Li Chao-Qun, Liu Jia, Jiang Bo-Fan
OBJECTIVE: To obtain the prokaryotic expression of transketolase genes and analyze its value as a diagnostic antigen for echinococcosis. METHODS: TK gene was amplified by PCR and cloned into prokaryotic vector pMD19-EgTK, and then subcloned into the expression vector pET-28a. The target gene TK prokaryotic expression plasmid pET-28a was constructed and transferred into BL21. The purified protein was identified by SDS-PAGE and Western blotting. The blood samples of patients with cystic echinococcosis (CE group), alveolar echinococcosis (AE group) and healthy people (healthy group) were collected and detected by ELISA with the recombinant EgTK protein as a diagnostic antigen...
April 20, 2018: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
https://www.readbyqxmd.com/read/29769131/echinococcus-multilocularis-and-echinococcus-shiquicus-in-a-small-mammal-community-on-the-eastern-tibetan-plateau-host-species-composition-molecular-prevalence-and-epidemiological-implications
#9
Xu Wang, Jiayu Liu, Qingqiu Zuo, Zhiqiang Mu, Xiaodong Weng, Xiaohui Sun, Junyao Wang, Belgees Boufana, Philip S Craig, Patrick Giraudoux, Francis Raoul, Zhenghuan Wang
BACKGROUND: The eastern part of the Tibetan Plateau is now recognized as an endemic region with the highest reported human infection rates in the world of human alveolar echinococcosis (AE) caused by Echinococcus multilocularis. Existing epidemiological studies on AE have mainly focused on the synanthropic environment, while basic parasitological and ecological aspects in wildlife host species remain largely unknown, especially for small mammal hosts. Therefore, we examined small mammal host species composition, occurrence, and the prevalence of both E...
May 16, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29765999/genetic-variability-of-antigen-b8-1-among-echinococcus-granulosus-isolates-from-human-cattle-and-sheep-in-fars-province-southern-iran
#10
Asieh Fatemi Esfedan, Bahador Sarkari, Fataneh Mikaeili
Background: Cystic echinococcosis (CE), known as hydatid cyst, is a zoonotic parasitic infection caused by the larval stage of Echinococcus granulosus (E. granulosus) . Antigen B, the major component of hydatid cyst fluid, is encoded by members of a multigene family. The present study aimed to evaluate the genetic diversity of the gene encoding antigen B8/1 ( EgAgB8/1 ) among the main intermediate hosts of E. granulosus . Methods: Twenty-eight hydatid cyst isolates (10 sheep, 9 human, and 9 cattle) were collected in Fars province, Iran...
April 2018: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29753095/cerebral-cystic-echinococcosis-in-mongolian-children-caused-by-echinococcus-canadensis
#11
Orkhontuul Shirmen, Batbold Batchuluun, Avaajigmed Lkhamjav, Tugbayar Tseveen, Tsetsegdelger Munkhjargal, Tsogtsaikhan Sandag, Enkhsaikhan Lkhagvasuren, Tetsuya Yanagida, Yuji Nishikawa, Akira Ito
Recent molecular re-evaluation of Echinococcus granulosus, which causes cystic echinococcosis (CE), has revealed that it is not a single species, but instead consists of 5 cryptic species. Among them, E. granulosus (dog-sheep strain) is predominant (75%) followed by Echinococcus canadensis (22%). The major affected organs, in humans, are the liver (88%) and lungs (11%). Primary cerebral CE comprises less than 1% of all cases. As cerebral CE cases are rare, there are few reports with molecular confirmation of the causative species...
May 9, 2018: Parasitology International
https://www.readbyqxmd.com/read/29752648/human-cystic-echinococcosis-in-hungary-2000-2014-a-retrospective-case-series-analysis-from-a-single-center-study
#12
Balázs Dezsényi, Áron Somorácz, József Danka, István Kucsera, Thomas F E Barth, Adriano Casulli
PURPOSE: Cystic echinococcosis (CE) is a parasitic disease caused by the tapeworm Echinococcus granulosus sensu lato. Although in Hungary the disease is listed among reportable infections, inadequacies in the reporting practice of CE by clinicians and pathologists have resulted in underscoring of this disease. The aim of this study was to describe the epidemiological and clinical characteristics of CE in Hungary using a datasource other than the official records that are based mainly on serological data...
May 11, 2018: Infection
https://www.readbyqxmd.com/read/29751768/the-preclinical-evaluation-of-immunocontraceptive-vaccines-based-on-canine-zona-pellucida-3-czp3-in-a-mouse-model
#13
Ying Wang, Yijie Li, Beibei Zhang, Fuchun Zhang
BACKGROUND: Stray dogs are the reservoirs and carriers of rabies and are definitive hosts of echinococcosis. To control the overpopulation of stray dogs, zona pellucida 3 (ZP3), a primary receptor for sperm, is a potential antigen for developing contraceptive vaccines. To enhance the immune responses and contraceptive effects of canine ZP3 (cZP3), dog gonadotropin-releasing hormone (GnRH) and a T cell epitope of chicken ovalbumin (OVA) were selected to construct two fusion proteins with cZP3, ovalbumin-GnRH-ZP3 (OGZ) and ovalbumin-ZP3 (OZ), and their contraceptive effects were evaluated in mice...
May 11, 2018: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/29746870/in-vitro-and-in-vivo-efficacies-of-carbazole-aminoalcohols-in-the-treatment-of-alveolar-echinococcosis
#14
Zhisheng Dang, Shuo Xu, Haobing Zhang, Weifeng Gui, Yumin Zhao, Liping Duan, Wei Hu
Benzimidazoles, including albendazole and mebendazole, are the major drugs for clinical chemotherapy of echinococcosis. They mainly exert parasitostatic effects depending on high dosages for long-term. Previous studies have identified carbazole aminoalcohols as novel anti-CE (cystic echinococcosis) agents. However, it is still to be confirmed whether it is effective on alveolar echinococcosis (AE) or not. In the present study, efficacies of novel carbazole aminoalcohols, propylamine, R-propylamine and S-propylamine were evaluated under in vitro and in vivo conditions...
May 7, 2018: Acta Tropica
https://www.readbyqxmd.com/read/29741144/in-vitro-study-on-protoscolicidal-effect-of-methanolic-extract-of-allium-hirtifolium-on-protoscoleces-of-cystic-echinococcosis
#15
Z Shahamir Tabatabaei, S Dehshahri, M M Taghi, F Esfandiari, F S Sadjjadi, M Ebrahimipour, S M Sadjjadi
Surgery is the treatment of choice for treatment of human cystic echinococcosis/ hydatidosis which is one of the most important parasitic zoonoses worldwide. Due to the risk of cyst rupture during surgery a protoscolicidal agent is usually injected into the cyst before surgery to prevent secondary cyst formation in case of cyst rupture. Due to the side effects of chemical protoscolicidals agents attempts has been focused on herbal medicine.In this regard, Allium sativum has already been used; however, the protoscolicidal effect of Allium hirtifolium from Allium family has not been investigated, so far...
May 9, 2018: Infectious Disorders Drug Targets
https://www.readbyqxmd.com/read/29736732/prevalence-and-genotyping-of-echinococcus-granulosus-in-sheep-in-narok-county-kenya
#16
D O Odongo, C M Tiampati, E Mulinge, C K Mbae, R P Bishop, E Zeyhle, J Magambo, M Wasserman, P Kern, T Romig
Cystic echinococcosis (CE) is a zoonotic disease caused by the larval stage of Echinococcus granulosus species (sensu lato, s.l.). In East Africa, several species/strains occur in livestock, wildlife, and humans, but there is limited information on frequencies of infection by different genotypes in the various mammalian hosts. We have obtained data on E. granulosus infection prevalence in sheep sampled from abattoirs in Narok County, southern Kenya. We inspected carcasses for the presence of hydatid cysts in 180 sheep randomly selected in five sub-locations...
May 8, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29733994/major-hepatectomies-in-liver-cystic-echinococcosis-a-bi-centric-experience-retrospective-cohort-study
#17
Jose Manuel Ramia, Alejandro Serrablo, Mario Serradilla, Aylhin Lopez-Marcano, Roberto de la Plaza, Ana Palomares
Surgical treatment of liver cystic echinococcosis (LCE) could be conservative or radical. Radical surgery includes liver resection, but usually are minor hepatectomy in favourable segments. Experience in major hepatectomy (MH) for LCE is limited. METHODS: Retrospective study. PERIOD: January 2007-December 2014. INCLUSION CRITERIA: liver infestation with Echinococcus granulosus causing active or complicated cysts. Epidemiological, clinical, radiological and surgical data were studied...
May 4, 2018: International Journal of Surgery
https://www.readbyqxmd.com/read/29727269/cystic-echinococcosis-in-a-domestic-cat-felis-catus-in-italy
#18
Piero Bonelli, Gabriella Masu, Silvia Dei Giudici, Davide Pintus, Angela Peruzzu, Toni Piseddu, Cinzia Santucciu, Assunta Cossu, Nicola Demurtas, Giovanna Masala
Echinococcus granulosus sensu lato is a zoonotic agent with a life cycle consisting of definitive hosts (dogs and wild carnivores), and intermediate hosts (usually ungulates). Other animals and humans may accidentally ingest eggs and contract cystic echinococcosis, acting as aberrant hosts. A 3-year-old neutered female cat was brought to a veterinary practice in Sassari (Italy) with abdominal distension. Ultrasound showed multiple intraperitoneal vesicles, which on laparotomy were found to be metacestodes of E...
2018: Parasite: Journal de la Société Française de Parasitologie
https://www.readbyqxmd.com/read/29719047/echinococcus-granulosus-cyst-fluid-enhances-epithelial-mesenchymal-transition
#19
Ahmed A Mohammed, J Allen, M T Rogan
AIMS: Cystic Echinococcosis is characterised by fluid filled hydatid cysts in the liver and lungs. The cysts are surrounded by a host fibrous layer (the pericyst) which acts to isolate the parasite from surrounding tissues. Previous studies in liver cysts have indicated that the parasite may be stimulating fibrosis. The aim of this study was to investigate whether Hydatid Cyst Fluid (HCF) could influence the potential for fibrosis to occur in lung tissue by stimulating epithelial to mesenchymal transition (EMT) in a human lung epithelial cell line...
May 2, 2018: Parasite Immunology
https://www.readbyqxmd.com/read/29708080/immunohistochemical-observation-of-local-inflammatory-cell-infiltration-in-the-host-tissue-reaction-site-of-human-hydatid-cysts
#20
R Jafari, B Sanei, A Baradaran, M Kolahdouzan, B Bagherpour, H Yousofi Darani
The aim of this study was to evaluate the pattern of local immune cell infiltration in human cystic echinococcosis (CE) by identifying the subtypes of immune cells using immunohistochemistry (IHC). Fifty surgically removed hydatid cyst samples and surrounding tissues were collected from patients referred to Al-Zahra Hospital, Isfahan, Iran. IHC was performed on the surrounding host tissue of hydatid cysts using anti-human CD3, CD19, CD8, CD4, CD68, CD56, Ki-67 and Foxp3 (forkhead box P3) antibodies. The results were then compared to hepatocellular carcinoma and chronic hepatitis...
April 30, 2018: Journal of Helminthology
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