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Stefanie Amend, Roland Frankenberger, Susanne Lücker, Eugen Domann, Norbert Krämer
OBJECTIVES: The present study aimed to establish Lactobacillus casei in a completely automated Streptococcus mutans-based artificial mouth model and to investigate secondary caries inhibiting properties of glass ionomer cements / resin composite groups in vitro. METHODS: Sixty extracted, caries-free human third molars were used for preparation of standardized class-V-cavities. Specimens were restored with a resin-modified (Photac Fil; PF) as well as a conventional glass ionomer cement (Ketac Molar; KM) and one resin composite bonded with and without conduction of etch-and-rinse technique (Prime&Bond NT, Ceram X mono; C+ERT, C)...
March 12, 2018: Dental Materials: Official Publication of the Academy of Dental Materials
S C Ibeneme, C Ekanem, A Ezuma, N Iloanusi, N N Lasebikan, O A Lasebikan, O E Oboh
BACKGROUND: Depletion of ovarian hormone in postmenopausal women has been associated with changes in the locomotor apparatus that may compromise walking function including muscle atrophy/weakness, weight gain, and bone demineralization. Therefore, handgrip strength (HGS), bone mineral density (BMD) and body composition [percentage body fat mass (%BFM), fat mass (FM), Fat-free mass (FFM) and body mass index (BMI)], may significantly vary and predict WB in postmenopausal women. Consequently, the study sought to 1...
March 15, 2018: BMC Musculoskeletal Disorders
Thanyaporn Kangwannaronkul, Keskanya Subbalekha, Philaiporn Vivatbutsiri, Jaijam Suwanwela
PURPOSE: Bio-Oss and demineralized freeze-dried bone allograft (DFDBA) are two commercial bone grafts that have been associated with clinical success for many years. However, there are few in vivo studies regarding their healing mechanism. The purpose of this study was to investigate the level of bone formation using microcomputed tomography (micro-CT) and gene expression in mouse calvaria at 1 and 3 months after bone grafting with deproteinized bovine bone and freeze-dried human bone, and compare them to natural bone healing...
March 15, 2018: International Journal of Oral & Maxillofacial Implants
Yan Liu, Ling Zhang, Li-Na Niu, Tao Yu, Hockin H K Xu, Michael D Weir, Thomas W Oates, Franklin R Tay, Ji-Hua Chen
OBJECTIVES: To evaluate the bonding performance, antibacterial activity, and remineralization effect on enamel of the orthodontic adhesive containing MAE-DB and NACP. METHODS: Eighty non-carious human premolars were divided into 3 groups: Transbond XT (TB), PEHB + 5% MAE-DB (PD), and PEHB + 40% NACP + 5% MAE-DB (PND). Premolars were bonded with orthodontic brackets, the first subgroup (n = 10) and the second subgroup (n = 10) were subjected to shear bond strength testing after immersed in water for 1 day and in demineralization solution for 28 days respectively and then tested surface roughness, while the third subgroup (n = 6) was used for microhardness evaluation after aged in demineralization solution for 28 days...
March 10, 2018: Journal of Dentistry
Xue Li, Jun Cao, Xinpeng Bai, Zefang Jiang, Xuanri Shen
Human milk fat substitutes (HMFS) are rich in polyunsaturated fatty acids which upon microencapsulation, can be used as a source of high quality lipids in infant formula. The response surface methodology (RSM) was employed to optimize the microencapsulation condition of HMFS as a functional product. The microencapsulation efficiency (MEE) of microencapsulated HMFS was investigated with respect to four variables including concentration of soy lecithin (A), ratio of demineralized whey powder to malt dextrin (B), HFMS concentration (C), and homogenizing pressure (D)...
March 9, 2018: Journal of Oleo Science
J Silveira, S Coutinho, D Marques, J Castro, A Mata, M L Carvalho, S Pessanha
In this work we present the analysis of dental enamel treated with an over-the-counter whitening product, bought in e-commerce at a very low cost, used without medical supervision in an abusive manner, in order to evaluate its demineralization action. Moreover, we studied the influence of renewal or non-renewal of saliva solution in which the specimens were stored throughout the study. The Degree of Demineralization was determined through the evaluation of the PO4 3- symmetric stretching band (~959cm-1 ) in Raman spectra of the specimens in different days during the course of the study...
March 5, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Chien Thang Doan, Thi Ngoc Tran, Van Bon Nguyen, Anh Dzung Nguyen, San-Lang Wang
Chitosanases and proteases have received much attention due to their wide range of applications. Four kinds of chitinous materials, squid pens, shrimp heads, demineralized shrimp shells and demineralized crab shells, were used as the sole carbon and nitrogen (C/N) source to produce chitosanases, proteases and α-glucosidase inhibitors (αGI) by four different strains of Paenibacillus . Chitosanase productivity was highest in the culture supernatants using squid pens as the sole C/N source. The maximum chitosanase activity of fermented squid pens (0...
March 8, 2018: Marine Drugs
Petra Korlević, Sahra Talamo, Matthias Meyer
Current protocols for ancient DNA and radiocarbon analysis of ancient bones and teeth call for multiple destructive samplings of a given specimen, thereby increasing the extent of undesirable damage to precious archaeological material. Here we present a method that makes it possible to obtain both ancient DNA sequences and radiocarbon dates from the same sample material. This is achieved by releasing DNA from the bone matrix through incubation with either EDTA or phosphate buffer prior to complete demineralization and collagen extraction utilizing the acid-base-acid-gelatinization and ultrafiltration procedure established in most radiocarbon dating laboratories...
March 7, 2018: Scientific Reports
Carianne Mendes de Almeida, Carine Tais Welter Meereis, Fernanda Barbosa Leal, Aline Oliveira Ogliari, Evandro Piva, Fabrício A Ogliari
This study evaluated the shear bond strength (SBS) of self-adhesive resin cements (SARCs) to dentin and their physical-chemical properties. Five commercial SARCs were evaluated [SmartCem®2 - DENTSPLY (SC2); BisCem® - Bisco (BC); SeT PP® - SDI (SeT); Relyx U100® - 3M ESPE (U100) and YCEM® SA - Yller (YCEM)]. The SARCs were evaluated for SBS to dentin (n = 10) after 24 h, 6 months, and 12 months. The dentin demineralization caused by acidic monomers was observed by SEM, and pH-neutralization of eluate was observed for 24 h...
March 1, 2018: Brazilian Oral Research
M Sauvan, N Chabbert-Buffet, M Canis, P Collinet, X Fritel, S Geoffron, G Legendre, J-M Wattier, H Fernandez
OBJECTIVE: To provide clinical practice guidelines for the management of painful endometriosis in women without infertility. METHODS: Systematic review of the literature literature since 2006, level of evidence rating, external proofreading and grading of the recommendation grade by an expert group according to HAS methodology. RESULTS: Combined hormonal contraceptives (COP) and the levonorgestrel-releasing intra-uterin system (LNG-IUS) are recommended as first-line hormonal therapies for the treatment of painful endometriosis (grade B)...
March 3, 2018: Gynecologie, Obstetrique, Fertilite & Senologie
Amel Slimani, Delphine Tardivo, Ivan V Panayotov, Bernard Levallois, Csilla Gergely, Frederic Cuisinier, Hervé Tassery, Thierry Cloitre, Elodie Terrer
Dentin carious lesion is a dynamic process that involves demineralization and collagen denaturation. Collagen type I is the major protein in dentin and it has been investigated based on its optical properties. Multiphoton microscopy (MPM) is a nonlinear imaging technique that reveals the caries process using the collagen two-photon excitation fluorescence (2PEF) and its second-harmonic generation (SHG). Combining the histological and the International Caries Detection and Assessment System (ICDAS) classifications with nonlinear optical spectroscopy (NLOS), 2PEF and SHG intensities of enamel and dentin were highly altered during the caries process...
March 6, 2018: Caries Research
Wadih Abou Hamdan, Sherine Badri, Ahmed El Sayed
AIM: The aim of this study was to evaluate in vitro the effectiveness of fluoride Vanish™ in preventing enamel demineralization around and under orthodontic brackets, using a laser fluorescence monitoring device on a group of teeth, using a laser fluorescence monitoring device on a group of extracted teeth with bonded brackets. METHODS: Orthodontic brackets were bonded to 32 extracted premolars. Buccal surfaces of teeth were divided into two halves: mesial and distal, fluoride Vanish™ (3M Unitek, Monrovia, CA, USA) was applied only on the distal half while the mesial one served as control half...
March 1, 2018: International Orthodontics
Albert Ngo, Kenneth H Chan, Oanh Le, Jacob C Simon, Daniel Fried
Recent studies have shown that near-IR (NIR) imaging methods such as NIR reflectance can be used to image lesions on proximal surfaces, and optical coherence tomography (OCT) can be used to measure the depth of those lesions below the tooth surface. These imaging modalities can be used to acquire high contrast images of demineralized tooth surfaces, and 2-D and 3-D images can be extracted from this data. At NIR wavelengths longer than 1200-nm, there is no interference from stains and the contrast is only due to the increased light scattering of the demineralization...
January 2018: Proceedings of SPIE
Jacob C Simon, Donald A Curtis, Cynthia L Darling, Daniel Fried
In vivo and in vitro studies have demonstrated that near-infrared (NIR) light at λ=1300-1700-nm can be used to acquire high contrast images of enamel demineralization without interference of stains. The objective of this study was to determine if a relationship exists between the NIR image contrast of occlusal lesions and the depth of the lesion. Extracted teeth with varying amounts of natural occlusal decay were measured using a multispectral-multimodal NIR imaging system which captures λ=1300-nm occlusal transillumination, and λ=1500-1700-nm cross-polarized reflectance images...
January 2018: Proceedings of SPIE
Jakob M Townsend, Brian T Andrews, Yi Feng, Jinxi Wang, Randolph J Nudo, Erik Van Kampen, Stevin H Gehrke, Cory J Berkland, Michael S Detamore
Traumatic brain injury (TBI) is a life-threatening condition defined by internal brain herniation. Severe TBI is commonly treated by a two-stage surgical intervention, where decompressive craniectomy is first conducted to remove a large portion of calvarial bone and allow unimpeded brain swelling. In the second surgery, spaced weeks to months after the first, cranioplasty is performed to restore the cranial bone. Hydrogels with paste-like precursor solutions for surgical placement may potentially revolutionize TBI treatment by permitting a single-stage surgical intervention, capable of being implanted with the initial surgery, remaining pliable during brain swelling, and tuned to regenerate calvarial bone after brain swelling has subsided...
February 26, 2018: Acta Biomaterialia
Hengameh Khosropanah, Nazila Lashkarizadeh, Maryam Ayatollahi, Maryam Kaviani, Zohreh Mostafavipour
Statement of the Problem: A great challenge in periodontal therapy is the regeneration enhancement of osseous defects through applying osteoinductive materials. Demineralized freeze-dried bone allograft (DFDBA) has already been introduced as an allograft with osteoconductive and variable osteoinductive properties. Calcium hydroxide [Ca(OH)2 ] is an available well-known material in dentistry, which induces hard tissue formation. Purpose: This study evaluated the efficiency of combination of DFDBA and Ca(OH)2 in improving the quality of osteoinduction of DFDBA...
March 2018: Journal of Dentistry
Roda Seseogullari-Dirihan, Meltem Tekbas Atay, David Henry Pashley, Arzu Tezvergil-Mutluay
The aim of this study was to evaluate the effect of curcuminoids on the dentin endogenous protease activity. Demineralized dentin were pretreated with 50 or 100 µM of three different curcuminoids for 60 s and incubated up to 3 months. Untreated beams served as controls. Dry dentin mass was measured after incubation. Aliquots were analyzed for the quantity of ICTP and CTX releases for MMP and cathepsin-K mediated degradation, respectively. The effect of curcuminoids on matrix-bound MMP and soluble rhMMP-9 were measured using an activity assay...
February 27, 2018: Dental Materials Journal
Beatriz Martines de Souza, Constantino Fernandes Neto, Priscila Maria Aranda Salomão, Layla Reginna Silva Munhoz de Vasconcelos, Flaviana Bombarda de Andrade, Ana Carolina Magalhães
Titanium tetrafluoride (TiF4) is known for interacting with enamel reducing demineralization. However, no information is available about its potential antimicrobial effect. OBJECTIVES: This study evaluated the antimicrobial and anti-caries potential of TiF4 varnish compared to NaF varnish, chlorhexidine gel (positive control), placebo varnish and untreated (negative controls) using a dental microcosm biofilm model. MATERIAL AND METHODS: A microcosm biofilm was produced on bovine enamel previously treated with the varnishes, using inoculum from human saliva mixed with McBain saliva, under 0...
2018: Journal of Applied Oral Science: Revista FOB
Thomas Thurnheer, Georgios N Belibasakis
OBJECTIVE: Fluoride is widely used as an anti-caries agent, e.g. in toothpastes and mouth rinses. However, the nature of the anti-caries action is not entirely clear. Mechanisms suspected to explain the cariostatic effect include inhibitory effects on acid formation by bacteria, inhibition of extracellular polysaccharide (EPS) production, inhibition of enamel demineralization and enhancement of remineralizaton or combination thereof. The aim of this study was to examine with the supragingival Zurich in vitro biofilm model the effect of fluoride in NaF formulation, on the microbiota and on demineralization...
February 20, 2018: Archives of Oral Biology
A Lederer, K H Kunzelmann, R Hickel, F Litzenburger
The purpose was to develop an in vitro model for the validation of near-infrared transillumination (NIRT) for proximal caries detection, to enhance NIRT with high-dynamic-range imaging (HDRI), and to compare both methods, using micro-computed tomography (µCT) as a reference standard. Both proximal surfaces of 53 healthy or decayed permanent human teeth were examined using the Diagnocam (DC) (KaVo) and NIRT with HDRI (NIRT-HDRI). NIRT was combined with HDRI to improve the diagnostic performance by reducing under- and overexposed image areas...
February 1, 2018: Journal of Dental Research
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