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H Askar, F Schwendicke, J Lausch, H Meyer-Lueckel, S Paris
OBJECTIVE: Infiltrant resin (IR) is currently indicated for non-cavitated caries lesions. However, modifying the technique might expand its indication spectrum to micro-cavitated lesions. The present study aimed to evaluate the penetration/filling ability of a newly developed micro-filled infiltrant resin (MFIR) in non-, micro- and cavitated natural caries lesions. MATERIALS AND METHODS: Proximal lesions in 120 extracted human teeth with ICDAS-2 (n = 30), 3 (n = 45) and 5 (n = 45) lesions were etched with 15% hydrochloric acid gel for 2 min and allocated to one of the following treatments; IR: lesions (ICDAS-2, 3 and 5; each n = 15) were treated with commercial infiltrant resin for 3 min...
May 15, 2018: Journal of Dentistry
Hyeong-Seop Jung, Min Hee Kim, Ji Youn Shin, Se Ra Park, Ju-Young Jung, Won Ho Park
A nanofibrous β-chitin web was fabricated via electrospinning for use as a novel wound dressing material. β-chitin was extracted from cuttlefish bone using deproteinization and demineralization. First, cuttlefish bone was alkali-treated to remove the proteins and was then treated with the acid for demineralization. The extracted β-chitin was dissolved in formic acid as solvent to evaluate its electrospinnability, and the electrospinnability increased remarkably when β-chitin was blended with poly(ethylene oxide) (PEO) than without...
August 1, 2018: Carbohydrate Polymers
Yasmin Etienne Albuquerque, Marcelle Danelon, Marcos José Salvador, Cristiane Yumi Koga-Ito, Alberto Carlos Botazzo Delbem, Román Yesid Ramirez-Rueda, Aline Vidal Lacerda Gontijo, Fernanda Lourenção Brighenti
AIM: To validate an in vitro caries model and to evaluate an experimental mouthwash containing Croton doctoris essential oil.  Materials & methods: To validate the experimental model, we used McBain medium and polymicrobial biofilms. The EOM (essential oil mouthwash) was tested using the validated model. Microbial composition (colony-forming unit/ml), acidogenicity, enamel demineralization (percentage of surface enamel hardness loss), cytotoxicity and essential oil composition were evaluated...
May 17, 2018: Future Microbiology
Luiz Carlos Ferreira da Silva, Gabriela Granja Porto, Emanuel Sávio de Souza Andrade, José Rodrigues Laureano Filho
PURPOSE: To compare bone regeneration in critical-sized defects in rat calvarium using demineralized bone matrix and calcium phosphate cement. METHODS: Thirty Wistar rats were divided into 3 groups of 10 animals each. Two defects of 5-mm were made in the parietal bones of each animal. Group I had calcium phosphate cement placed in the experimental defect, Group II had filled with demineralized bone matrix and Group III had with the combination of the matrix and cement in equal parts...
April 2018: Acta Cirúrgica Brasileira
Maria Isabel Dantas de Medeiros, Hugo Lemes Carlo, Rogério Lacerda Dos Santos, Frederico Barbosa Sousa, Ricardo Dias de Castro, Renata Cristina Sobreira França, Fabíola Galbiatti de Carvalho
The effect of fluoride agents on the retention of orthodontic brackets to enamel under erosive challenge is little investigated. The aim of this study was to evaluate the effect of titanium tetrafluoride (TiF4) and sodium fluoride (NaF) agents on the shear bond strength of brackets to enamel and on the enamel microhardness around brackets under erosive challenge. Brackets were bonded to bovine incisors. Five groups were formed according to fluoride application (n=10): TiF4 varnish, TiF4 solution, NaF varnish, NaF solution and control (without application)...
May 14, 2018: Journal of Applied Oral Science: Revista FOB
Hee-Eun Kim, Baek-Il Kim
PURPOSE: The aim of thisin situ study was to measure baseline fluorescence loss values (ΔF(0) ) using the quantitative light-induced fluorescence (QLF) technology to screen enamel lesions for effective remineralization following fluoride application. METHODS: In this single-blindedin situ study, 20 adult volunteers wore intraoral appliances containing 4 specimens of human enamel. The surfaces of the specimens were divided into 3 regions: sound, demineralized, and treated regions...
May 12, 2018: Photodiagnosis and Photodynamic Therapy
M Winters, A Torkelson, R Booth, C Mailand, Y Hoareau, S Tucker, S K Wasser
Genotyping ivory samples can determine the geographic origin of poached ivory as well as the legality of ivory being sold in ivory markets. We conducted a series of experiments to determine where the DNA is most concentrated in ivory samples and how best to increase DNA yield from groups of samples likely to vary in DNA concentration. We examined variation in DNA amplification success from: the layer(s) of the tusk (cementum and/or dentine) being extracted, demineralization temperature and time, and the concentration of eluates...
April 27, 2018: Forensic Science International
Amsaveni Ramachandran, Kun He, Chun-Chieh Huang, Reza Shahbazian-Yassar, Tolou Shokuhfar, Anne George
In search for bone and dentin extracellular matrix (ECM) proteins, transforming growth factor beta receptor II interacting protein 1 (TRIP-1) was identified as a novel protein synthesized by osteoblasts and odontoblasts and exported to the ECM. TRIP-1 is a WD-40 (WD is Tryptophan-Aspartic acid dipeptide) protein that has been well recognized for its physiological role in the endoplasmic reticulum (ER). In the ER, TRIP-1 functions as an essential subunit of eukaryotic elongation initiation factor 3 and is involved in the protein translational machinery...
December 2018: Connective Tissue Research
Mohammad Tariqur Rahman, Ashfaque Hossain, Chew Hooi Pin, Noor Azlin Yahya
Chronic oxidative stress and reactive oxygen species (ROS) in oral cavity as well as acidic pH on dental enamel surface due to the metabolic activities of bacterial plaque are the major contributors in the development and progression of dental caries. Along with other factors, deposition or dissolution Ca and Mg mostly determines the re- or demineralization of dental enamel. Zn plays an important role for both Ca and Mg bioavailability in oral cavity. Metallothionein (MT), a group of small molecular weight, cysteine-rich proteins (~ 7 kDa), is commonly induced by ROS, bacterial infection, and Zn...
May 9, 2018: Biological Trace Element Research
Katelyn Elizabeth Mason, Deon Anex, Todd Grey, Bradley Hart, Glendon Parker
Bone tissue contains organic material that is useful for forensic investigations and may contain preserved endogenous protein that can persist in the environment for extended periods of time over a range of conditions. Single amino acid polymorphisms in these proteins reflect genetic information since they result from non-synonymous single nucleotide polymorphisms (SNPs) in DNA. Detection of genetically variant peptides (GVPs) - those peptides that contain amino acid polymorphisms - in digests of bone proteins allows for the corresponding SNP alleles to be inferred...
April 22, 2018: Forensic Science International
Rana Alkattan, Frank Lippert, Qing Tang, George J Eckert, Masatoshi Ando
OBJECTIVES: The objectives were to investigate the hardness and chemical composition of sound, demineralized and pH-cycled bovine enamel and determine their influence on demineralization and remineralization behavior. METHODS: Ninety-four, 5 × 5 × 2-mm bovine enamel specimens were demineralized using three different times [(24 h (n = 33), 48 h (n = 30), 96 h (n = 31)]. The specimens were then pH-cycled using either 367 ppm F sodium fluoride or deionized water...
May 5, 2018: Journal of Dentistry
You-Min Kim, Dong-Hyun Kim, Chang Weon Song, Seog-Young Yoon, Se-Yeon Kim, Hee Sam Na, Jin Chung, Yong-Il Kim, Yong Hoon Kwon
Objective: The aim of this study was to evaluate the mechanical and biological properties of orthodontic bonding agents containing silver- or zinc-doped bioactive glass (BAG) and determine the antibacterial and remineralization effects of these agents. Methods: BAG was synthesized using the alkali-mediated solgel method. Orthodontic bonding agents containing BAG were prepared by mixing BAG with flowable resin. Transbond™ XT (TXT) and Charmfil™ Flow (CF) were used as controls...
May 2018: Korean Journal of Orthodontics
Kou Fujita-Nakajima, Naoko Aoki-Tabei, Akishi Arita, Norihiro Nishiyama
The acidic monomers utilized in all-in-one adhesives play a key role in the enamel and dentin bonding performance. The purpose of this study is to investigate the mechanism by which 10-methacryloyloxydecyl dihydrogen phosphate (MDP) demineralizes the enamel and dentin surfaces prepared by a diamond bur in three types of experimental MDP-based all-in-one (EX) adhesives containing different amounts of water (46.6, 93.2 and 208.1 mg/g). The enamel and dentin reactants of EX adhesives were analyzed using solidstate phosphorous-31 nuclear magnetic resonance and X-ray diffraction...
May 2, 2018: Dental Materials Journal
Masahiro Teshima
The effects of the water concentration in an experimental 10-methacryloyloxydecyl dihydrogen phosphate (MDP)-based all-in-one (EX) adhesive were examined on the ability of MDP to remove the smear layer from the ground dentin surface and on the dentin bonding performance. Four types of EX adhesives were prepared by varying the amount of water (46.6, 93.2, 149.8 and 208.1 mg/g), but the MDP concentration was kept a constant at 49.9 mg/g. Scanning electron microscopy and bond strength measurements were performed on the dentin surface demineralized by each EX adhesive...
May 2, 2018: Dental Materials Journal
Angel Y Sanchez, Caroline L de Oliveira, Thais C Negrini, Lina N Hashizume, Anderson T Hara, Marisa Maltz, Rodrigo A Arthur
There is limited evidence that arginine-containing fluoridated dentifrices (AFD) have a better anticaries effect than regular fluoridated dentifrices (FD), especially in subjects at a higher risk for caries development. This study aimed to assess the effect of AFD on enamel demineralization and on the microbial and biochemical compositions of biofilm formed under different frequencies of sucrose exposure. It consisted of an in situ split-mouth design, where 12 adult volunteers who used FD for at least 2 months prior to the beginning of this study wore acrylic palatal appliances containing 4 bovine enamel specimens (1 pair at each side of the appliance) during 2 phases of 14 days each...
May 4, 2018: Caries Research
Tisson V Job, Girish T Narayana, Kishan K Venkappa, K Binu Nathan, Shameem Ahsan, Jayakkodi Harikaran
Aim: The aim of this study was to compare the remineralization potential of three different dentifrices using Raman spectroscopy and confocal laser scanning microscopy (CLSM). Materials and methods: Totally, 30 extracted intact impacted third molar teeth were selected and the crown of each tooth in a group was separated from the root and longitudinally sectioned into four parts with each section under a subgroup, of which one section was an untreated section, the second and the third sections were demineralized in a demineralizing solution, and the third section was remineralized after demineralization...
April 1, 2018: Journal of Contemporary Dental Practice
Joseph R Greenberg, Stephen Sinclair, Cheryl A Janssen, Kevin Krick, Mimi O'Brien, Kate Flanagan, Michael A Posner
OBJECTIVE: To evaluate the reliability, ease, and efficiency of data entry for an oral health screening app that allows iPad® entry of data, including permanent versus deciduous teeth present, visual image comparison grading of demineralization/caries, fluorosis, periodontal inflammation, oral hygiene status, identification of sealants/restorations, dental trauma, orthodontic malocclusion, mandibular joint dysfunction, and early childhood caries. METHODS: 89 consented children were examined first by a public health dental hygienist in a dental office reception area and then by a dentist in a treatment operatory...
May 2018: Compendium of Continuing Education in Dentistry
Vani Damodaran, Nilesh J Vasa, R Sarathi
A high-speed 840 nm based polarization-sensitive time domain optical coherence tomography (PSOCT) technique is proposed and demonstrated based on the quadratic electro-optic property of potassium tantalate niobate (KTN) crystals. A longitudinal (axial) scanning depth of ≈10  μm is obtained for an applied AC voltage of 600 V, at 1000 Hz and temperature maintained around 40°C. The OCT system with the KTN-based electro-optic delay line combined with a linear actuation is extended to image an early dental demineralization...
April 10, 2018: Applied Optics
Antonio José Ortiz-Ruiz, Iban Jesús Muñoz-Gómez, Ana Pérez-Pardo, Concepción Germán-Cecilia, Yolanda Martínez-Beneyto, Ascensión Vicente
The aim was to evaluate the effect of fluoride varnish on the shear bond strength (SBS) on polished and non-polished intact and demineralized enamel. Bovine incisors (half demineralized) were used. Bifluorid 12™ was applied. Bonding was made with Futurabond® M + and GrandioSO, 24 h and 7 days after varnishing. In some groups, varnish was removed by polishing before bonding. SBS was measured. Fracture type was determined by stereomicroscopy and scanning electron microscope (SEM) observations of the enamel surface were made...
April 27, 2018: Odontology
T Shishniashvili, T Suladze, M Makhviladze, M Kalandaze, V Margvelashvili
OBJECTIVES: The goal of the present study correlates dental hard tissue mineralization, mucosal pathologies in the oral cavity and different degrees of intestinal dysbiosis. STUDY DESIGN: the study examined two groups: the study group (Group I) included 229 children and adolescents aged 1-17 (mean age 5±1years) with oral pathologies (caries, acute or chronic candidiasis) and confirmed dysbiosis of varying severity and stages as well. Group II (the Control Group) was composed of 50 patients aged 1 - 16 (mean age 5±1years) with oral pathologies but with no detected changes in gastrointestinal (GI) flora...
April 26, 2018: Journal of Clinical Pediatric Dentistry
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