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https://www.readbyqxmd.com/read/29779063/development-of-sperm-vitrification-protocols-for-two-endangered-salmonid-species-the-adriatic-grayling-thymallus-thymallus-and-the-marble-trout-salmo-marmoratus
#1
Eszter Kása, Jelena Lujić, Zoran Marinović, Tímea Kollár, Gergely Bernáth, Zoltán Bokor, Béla Urbányi, Kinga Katalin Lefler, Dušan Jesenšek, Ákos Horváth
Vitrification was applied to the sperm of two endangered fish species of Soča River basin in Slovenia, the Adriatic grayling (Thymallus thymallus) and marble trout (Salmo marmoratus) following testing different cooling devices and vitrifying media. Sperm was collected, diluted in species-specific non-activating media containing cryoprotectants, and vitrified by plunging directly into liquid nitrogen without pre-cooling. Progressive motility, curvilinear velocity, and straightness of fresh and vitrified-warmed sperm were evaluated with computer-assisted sperm analysis (CASA)...
May 19, 2018: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29560576/optimisation-of-sodium-and-potassium-concentrations-and-ph-in-the-artificial-seminal-plasma-of-common-carp-cyprinus-carpio-l
#2
Beata Irena Cejko, Ákos Horváth, Timea Kollár, Eszter Kása, Jelena Lujić, Zoran Marinović, Béla Urbányi, Radosław Kajetan Kowalski
The effect of sodium and potassium concentrations as well as optimal pH on the motility of common carp Cyprinus carpio L. sperm during short-term storage in artificial seminal plasma (ASP) was investigated. Sperm was collected from individual males (n = 5) and each sample diluted tenfold (1:9) in ASP (sperm:extender) containing 2 mM CaCl2 , 1 mM Mg2 SO4 and 20 mM Tris at pH 8.0 and supplemented by the following concentrations of sodium and potassium (mM/mM): 0/150, 20/130, 40/110, 75/75, 110/40, 130/20 and 150/0...
March 21, 2018: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29553568/cryopreservation-of-zebrafish-spermatogonia-by-whole-testes-needle-immersed-ultra-rapid-cooling
#3
Zoran Marinović, Jelena Lujić, Eszter Kása, Zsolt Csenki, Béla Urbányi, Ákos Horváth
Current trends in science and biotechnology lead to creation of thousands of new lines in model organisms thereby leading to the necessity for new methods for safe storage of genetic resources beyond the common practices of keeping breeding colonies. The main purpose of this study was to adapt the needle immersed vitrification (NIV) procedure to cryopreserve whole zebrafish testes. Cryopreservation of early-stage germ cells by whole testes NIV offers possibilities for the storage of zebrafish genetic resources, especially since after transplantation they can mature into both male and female gametes...
March 4, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29525864/development-of-an-in-vitro-toxicological-test-system-based-on-zebrafish-danio-rerio-sperm-analysis
#4
Tímea Kollár, Eszter Kása, Árpád Ferincz, Béla Urbányi, Zsolt Csenki-Bakos, Ákos Horváth
The effect of seven heavy metals on the motility parameter of zebrafish sperm was tested in order to develop an in vitro toxicological test system as an alternative to live animal testing. In vitro test systems are currently preferred in ecotoxicology due to their practical and ethical advantages and fish sperm can be a suitable model. A number of studies had been carried out previously on this topic, but the described methods had not been standardized in numerous aspects (donor species, measured endpoint, etc...
March 10, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29443049/handling-and-treatment-of-male-european-eels-anguilla-anguilla-for-hormonal-maturation-and-sperm-cryopreservation
#5
Juan G Herranz-Jusdado, Eszter Kása, Tímea Kollár, Víctor Gallego, David S Peñaranda, Christoffer Rozenfeld, Luz Pérez, Ákos Horváth, Juan F Asturiano
During the last years, several research groups have been working on the development and improvement of new protocols for the European eel handling and maturation. As of yet, weekly injections of human chorionic gonadotropin (hCG) have proved to maturate males after just 5-6 weeks of treatment, producing high volumes of high-quality sperm during several weeks. In addition, sperm cryopreservation protocols using different extenders, cryoprotectants and cooling and thawing times have been previously described for European eel...
January 28, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29359516/seminal-sirt1-expression-in-infertile-oligoasthenoteratozoospermic-men-with-varicocoele
#6
T Mostafa, N Nabil, L Rashed, K Makeen, M A El-Kasas, H A Mohamaed
In a case-controlled study, we assessed the expressed seminal NAD-dependent protein deacetylase (SIRT1) expression in infertile oligoasthenoteratozoospermic (OAT) men associated with varicocoele. Our study involved 81 men, recruited from the University hospitals, after ethical approval and informed consent. They were allocated into fertile normozoospermic men (n = 23), infertile OAT men without varicocoele (n = 23) and infertile OAT men with varicocoele (n = 35). Inclusion criteria consisted of confirmation of abnormal semen parameters and normal female partners whereas exclusion criteria were leukocytospermia, tobacco smoking, hormonal therapy, immunological disorders, dyslipidemia, hypogonadism, cardiovascular disorders, morbid obesity, and hepatic or renal failures...
March 2018: Andrology
https://www.readbyqxmd.com/read/29290106/design-synthesis-and-antifungal-activities-of-3-acyl-thiotetronic-acid-derivatives-new-fatty-acid-synthase-inhibitors
#7
Pei Lv, Yiliang Chen, Zheng Zhao, Taozhong Shi, Xiangwei Wu, Jiaying Xue, Qing X Li, Rimao Hua
Emerging fungal phytodiseases are increasingly becoming a food security threat. Twenty-six new 3-acylthiotetronic acid derivatives were designed, synthesized, characterized, and evaluated for activities against Valsa mali, Curvularia lunata, Fusarium graminearum, and Fusarium oxysporum f. sp. lycopersici. Among the 26 compounds, 6f was the most effective against V. mali, C. lunata, F. graminearum, and F. oxysporum f. sp. lycopersici with median effective concentrations (EC50 ) of 4.1, 3.1, 3.6, and 4.1 μg/mL, respectively, while the corresponding EC50 were 0...
January 31, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29205867/nanomotion-detection-method-for-testing-antibiotic-resistance-and-susceptibility-of-slow-growing-bacteria
#8
María Ines Villalba, Petar Stupar, Wojciech Chomicki, Massimiliano Bertacchi, Giovanni Dietler, Laura Arnal, María Elena Vela, Osvaldo Yantorno, Sandor Kasas
Infectious diseases are caused by pathogenic microorganisms and are often severe. Time to fully characterize an infectious agent after sampling and to find the right antibiotic and dose are important factors in the overall success of a patient's treatment. Previous results suggest that a nanomotion detection method could be a convenient tool for reducing antibiotic sensitivity characterization time to several hours. Here, the application of the method for slow-growing bacteria is demonstrated, taking Bordetella pertussis strains as a model...
January 2018: Small
https://www.readbyqxmd.com/read/29168172/extracellular-adenosine-induced-rac1-activation-in-pulmonary-endothelium-molecular-mechanisms-and-barrier-protective-role
#9
Anita Kovacs-Kasa, Kyung Mi Kim, Mary Cherian-Shaw, Stephen M Black, David J Fulton, Alexander D Verin
We have previously shown that Gs-coupled adenosine receptors (A2a) are primarily involved in adenosine-induced human pulmonary artery endothelial cell (HPAEC) barrier enhancement. However, the downstream events that mediate the strengthening of the endothelial cell (EC) barrier via adenosine signaling are largely unknown. In the current study, we tested the overall hypothesis that adenosine-induced Rac1 activation and EC barrier enhancement is mediated by Gs-dependent stimulation of cAMP-dependent Epac1-mediated signaling cascades...
August 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29055476/prevalence-of-cognitive-impairment-among-hungarian-patients-with-relapsing-remitting-multiple-sclerosis-and-clinically-isolated-syndrome
#10
Dániel Sandi, Tamás Biernacki, Dóra Szekeres, Judit Füvesi, Zsigmond Tamás Kincses, Csilla Rózsa, Klotild Mátyás, Krisztián Kása, Judit Matolcsi, Dóra Zboznovits, Zita Burány, Éva Langane, László Vécsei, Krisztina Bencsik
BACKGROUND: Cognitive impairment (CI) is a frequent symptom of multiple sclerosis (MS); its prevalence is reported to be 43-70%. It is one of the most important determinants of MS patients' quality of life, as it is one of the main factors for MS patients becoming unemployed. AIM: We aimed to determine the prevalence of CI among the relapsing-remitting MS (RRMS) and clinically isolated syndrome (CIS) patients in Hungary, to evaluate the predicting factors of CI and to assess the differences between sexes and patients with different educational levels...
October 2017: Multiple Sclerosis and related Disorders
https://www.readbyqxmd.com/read/29040289/sole-vibration-improves-locomotion-through-the-recovery-of-joint-movements-in-a-mouse-cast-model
#11
Atsushi Doi, Kazuaki Miyamoto, Yu-Shin Nakano, Juntaro Sakasaki, Syota Kasae, Keisuke Nishimura, Min-Chul Shin, Megumu Yoshimura
We investigated the effects of a vibratory stimulus on the plantar surface of the hind limb for motor, sensory, and locomotive function using a mouse cast model. The right knee joint of C57BL/6 male mice (7 weeks, 20 g, n = 31) was flexed with aluminum splint and tape for 6 weeks. These mice were randomly divided into 2 groups (control group, n = 11 and vibration group, n = 12). The mice in the vibration group received vibration on the sole of the ankle for 15 minutes per day, 5 days per week. After the knee joint cast was removed, we measured the range of motion (ROM) of both knee and ankle joints and the sensory threshold of the sole...
2017: PloS One
https://www.readbyqxmd.com/read/28993597/using-laser-interference-lithography-in-the-fabrication-of-a-simplified-micro-and-nanofluidic-device-for-label-free-detection
#12
Taiga Ajiri, Haruya Kasa, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Junji Nishii, Manabu Tokeshi
Recently, we developed a label-free detection method based on optical diffraction, and implemented it in on our fabricated micro- and nanofluidic device. This detection method is simple and useful for detecting biomolecules, but the device fabrication consists of complicated processes. In this paper, we propose a simple method for fabricating the micro- and nanofluidic device; the fabrication combines laser interference lithography with conventional photolithography. The performance of a device fabricated by the proposed method is comparable to the performance of the device in our previous study...
2017: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/28912165/holistic-care-of-complicated-tuberculosis-in-healthcare-settings-with-limited-resources
#13
REVIEW
Trevor Duke, Sharon Kasa Tom, Harry Poka, Henry Welch
In recent years, most of the focus on improving the quality of paediatric care in low-income countries has been on improving primary care using the Integrated Management of Childhood Illness, and improving triage and emergency treatment in hospitals aimed at reducing deaths in the first 24 hours. There has been little attention paid to improving the quality of care for children with chronic or complex diseases. Children with complicated forms of tuberculosis (TB), including central nervous system and chronic pulmonary TB, provide examples of acute and chronic multisystem paediatric illnesses that commonly present to district-level and second-level referral hospitals in low-income countries...
December 2017: Archives of Disease in Childhood
https://www.readbyqxmd.com/read/28856604/in-silico-drug-design-of-thiolactomycin-derivatives-against-mtb-kasa-enzyme-to-inhibit-multidrug-resistance-of-mycobacterium-tuberculosis
#14
D Ruban Durairaj, P Shanmughavel
Tuberculosis (TB) is a leading infectious disease which kills a huge number of people every year over a decade, caused by Mycobacterium tuberculosis. The conventional drugs in the market are no longer effective due to the increasing mycobacterial resistance to antibiotics. Hence, the need of finding efficient drugs to solve this multiple drug resistant factor is becoming an immediate issue. The first-line drugs in current practice for the treatment of TB emphasize on mycolic acid, which protects the bacteria from an immune response generated by the host...
August 30, 2017: Interdisciplinary Sciences, Computational Life Sciences
https://www.readbyqxmd.com/read/28845298/amyloid-single-cell-cytotoxicity-assays-by-nanomotion-detection
#15
Francesco S Ruggeri, Anne-Laure Mahul-Mellier, Sandor Kasas, Hilal A Lashuel, Giovanni Longo, Giovanni Dietler
Cells are extremely complex systems able to actively modify their metabolism and behavior in response to environmental conditions and stimuli such as pathogenic agents or drugs. The comprehension of these responses is central to understand the molecular bases of human pathologies, including amyloid misfolding diseases. Conventional bulk biological assays are limited by intrinsic cellular heterogeneity in gene, protein and metabolite expression, and can investigate only indirectly cellular reactions in non-physiological conditions...
2017: Cell Death Discovery
https://www.readbyqxmd.com/read/28830743/afm-contribution-to-unveil-pro-and-eukaryotic-cell-mechanical-properties
#16
REVIEW
S Kasas, P Stupar, G Dietler
Atomic force microscopy is nowadays a well-established technique that permits the investigation of numerous parameters of living matter. In particular, it allows the exploration of the mechanical properties of living organisms in almost physiological conditions. Here, we focus on the use of this technology to review recent contributions that relates the physiology and pathology of bacteria, yeast, plant and mammalian cells to their nano-mechanical properties.
January 2018: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/28819569/a-study-on-il8rb-gene-polymorphism-as-a-potential-immuno-compromised-adherent-in-exaggeration-of-parenteral-and-mammo-crine-oxidative-stress-during-mastitis-in-buffalo
#17
S M El Nahas, A H El Kasas, A A Abou Mossallem, M I Abdelhamid, Mohamad Warda
The genetic markers in inflammatory responses during mastitis afford a reasonable way for improving milk production in the Egyptian buffalo breed. Among them is the interleukin 8 Receptor Gene (IL8RB) (CXCR2); a chemokine receptor gene augments the neutrophil migration during infection. To understand its role better during mastitis in Egyptian buffalos, twenty-five dairy animals representing the normal, sub-clinically, clinically and chronically affected buffalos were randomly selected from different districts...
November 2017: Journal of Advanced Research
https://www.readbyqxmd.com/read/28730192/first-stereoselective-total-synthesis-of-brevipolide-m
#18
Kasa Shiva Raju, Gowravaram Sabitha
The first stereoselective total synthesis of a cytotoxic brevipolide M, which shares a pyrone framework bearing a tetrahydrofuran moiety and a cinnamate group with the readily available (-)-DET, is described. The key steps involved in the synthesis are the epoxide-opening, Brown's allylation, and the RCM reaction to install an α,β-unsaturated lactone ring and the inversion of the C-6' stereogenic hydroxyl group using the Mitsunobu reaction.
August 2, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28719068/correlation-between-the-proportion-of-stained-eggs-and-the-number-of-mites-dermanyssus-gallinae-monitored-using-a-non-parallel-board-trap
#19
Makiko Odaka, Kazumasa Ogino, Michitaka Shikada, Kenichi Asada, Syoujirou Kasa, Takahiro Inoue, Ken Maeda
The poultry red mite (Dermanyssus gallinae) is a serious problem for the poultry industry worldwide. However, the relationship between the mite population and the damage that they cause is still unclear. In this study, the mite population in poultry houses was examined using an established trap method, and the risk of blood-stained eggs caused by the mites was assessed. Traps were placed once a week outside the egg channels and/or on the floor in two poultry farms in Fukuoka Prefecture, Japan, from April 2012 to July 2014...
December 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28716606/dna-protein-interactions-explored-by-atomic-force-microscopy
#20
REVIEW
S Kasas, G Dietler
DNA-protein interactions play an important role in all living organisms on Earth. The advent of atomic force microscopy permitted for the first time to follow and to characterize interaction forces between these two molecular species. After a short description of the AFM and its imaging modes we review, in a chronological order some of the studies that we think importantly contributed to the field.
January 2018: Seminars in Cell & Developmental Biology
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