keyword
https://read.qxmd.com/read/26109069/factor-b-is-the-second-lipopolysaccharide-binding-protease-zymogen-in-the-horseshoe-crab-coagulation-cascade
#21
JOURNAL ARTICLE
Yuki Kobayashi, Toshiaki Takahashi, Toshio Shibata, Shunsuke Ikeda, Takumi Koshiba, Hikaru Mizumura, Toshio Oda, Shun-ichiro Kawabata
Factor B is a serine-protease zymogen in the horseshoe crab coagulation cascade, and it is the primary substrate for activated factor C, the LPS-responsive initiator of the cascade. Factor C is autocatalytically activated to α-factor C on LPS and is artificially converted to β-factor C, another activated form, by chymotrypsin. It is not known, however, whether LPS is required for the activation of factor B. Here we found that wild-type factor B expressed in HEK293S cells is activated by α-factor C, but not by β-factor C, in an LPS-dependent manner and that β-factor C loses the LPS binding activity of factor C through additional cleavage by chymotrypsin within the N-terminal LPS-binding region...
July 31, 2015: Journal of Biological Chemistry
https://read.qxmd.com/read/25521624/first-asian-invertebrate-immunity-symposium-busan-korea-preface-to-the-special-issue
#22
Bok Luel Lee, Shun-ichiro Kawabata, Chengshu Wang, Yonggyun Kim
No abstract text is available yet for this article.
January 2015: Archives of Insect Biochemistry and Physiology
https://read.qxmd.com/read/25140902/influenza-a-virus-protein-pb1-f2-translocates-into-mitochondria-via-tom40-channels-and-impairs-innate-immunity
#23
JOURNAL ARTICLE
Takuma Yoshizumi, Takeshi Ichinohe, Osamu Sasaki, Hidenori Otera, Shun-ichiro Kawabata, Katsuyoshi Mihara, Takumi Koshiba
Mitochondria contribute to cellular innate immunity against RNA viruses. Mitochondrial-mediated innate immunity is regulated by signalling molecules that are recruited to the mitochondrial membrane, and depends on the mitochondrial inner membrane potential (Δψm). Here we examine the physiological relevance of Δψm and the mitochondrial-associating influenza A viral protein PB1-F2 in innate immunity. When expressed in host cells, PB1-F2 completely translocates into the mitochondrial inner membrane space via Tom40 channels, and its accumulation accelerates mitochondrial fragmentation due to reduced Δψm...
August 20, 2014: Nature Communications
https://read.qxmd.com/read/25077965/the-n-terminal-arg-residue-is-essential-for-autocatalytic-activation-of-a-lipopolysaccharide-responsive-protease-zymogen
#24
JOURNAL ARTICLE
Yuki Kobayashi, Takafumi Shiga, Toshio Shibata, Miyuki Sako, Katsumi Maenaka, Takumi Koshiba, Hikaru Mizumura, Toshio Oda, Shun-ichiro Kawabata
Factor C, a serine protease zymogen involved in innate immune responses in horseshoe crabs, is known to be autocatalytically activated on the surface of bacterial lipopolysaccharides, but the molecular mechanism of this activation remains unknown. In this study, we show that wild-type factor C expressed in HEK293S cells exhibits a lipopolysaccharide-induced activity equivalent to that of native factor C. Analysis of the N-terminal addition, deletion, or substitution mutants shows that the N-terminal Arg residue and the distance between the N terminus and the tripartite of lipopolysaccharide-binding site are essential factors for autocatalytic activation, and that the positive charge of the N terminus may interact with an acidic amino acid(s) of the molecule to convert the zymogen into an active form...
September 12, 2014: Journal of Biological Chemistry
https://read.qxmd.com/read/24389234/interaction-between-tachyplesin-i-an-antimicrobial-peptide-derived-from-horseshoe-crab-and-lipopolysaccharide
#25
JOURNAL ARTICLE
Takahiro Kushibiki, Masakatsu Kamiya, Tomoyasu Aizawa, Yasuhiro Kumaki, Takashi Kikukawa, Mineyuki Mizuguchi, Makoto Demura, Shun-ichiro Kawabata, Keiichi Kawano
Lipopolysaccharide (LPS) is a major constituent of the outer membrane of Gram-negative bacteria and is the very first site of interactions with antimicrobial peptides (AMPs). In order to gain better insight into the interaction between LPS and AMPs, we determined the structure of tachyplesin I (TP I), an antimicrobial peptide derived from horseshoe crab, in its bound state with LPS and proposed the complex structure of TP I and LPS using a docking program. CD and NMR measurements revealed that binding to LPS slightly extends the two β-strands of TP I and stabilizes the whole structure of TP I...
March 2014: Biochimica et Biophysica Acta
https://read.qxmd.com/read/24213008/expression-and-functional-characterization-of-recombinant-tributyltin-binding-protein-type-2
#26
JOURNAL ARTICLE
Hina Satone, Eriko Akahoshi, Aiko Nakamura, Jae Man Lee, Masato Honda, Yohei Shimasaki, Shun-Ichiro Kawabata, Takahiro Kusakabe, Yuji Oshima
Tributyltin-binding proteins (TBT-bps) are members of the fish lipocalins that were isolated from the blood of Japanese flounder (Paralichthys olivaceus) and function in the binding and detoxification of TBT. In this study, we constructed a baculovirus-silkworm expression system and obtained recombinant TBT-bp2 (rTBT-bp2; 31.5 kDa) from the hemolymph of silkworm larvae injected with a recombinant baculovirus containing the TBT-bp2 gene. The binding potential of rTBT-bp2 was investigated and compared to that of the previously available recombinant TBT-bp1 (rTBT-bp1)...
2013: Journal of Toxicological Sciences
https://read.qxmd.com/read/23882120/transglutaminase-catalyzed-protein-protein-cross-linking-suppresses-the-activity-of-the-nf-%C3%AE%C2%BAb-like-transcription-factor-relish
#27
JOURNAL ARTICLE
Toshio Shibata, Sanae Sekihara, Takumi Fujikawa, Ryuta Miyaji, Kouki Maki, Takeshi Ishihara, Takumi Koshiba, Shun-ichiro Kawabata
Cross-linking of proteins by mammalian transglutaminases (TGs) plays important roles in physiological phenomena such as blood coagulation and skin formation. We show that Drosophila TG suppressed innate immune signaling in the gut. RNA interference (RNAi) directed against TG reduced the life span of flies reared under conventional nonsterile conditions but not of those raised under germ-free conditions. In conventionally reared flies, TG RNAi enhanced the expression of genes encoding antimicrobial peptides in the immune deficiency (IMD) pathway...
July 23, 2013: Science Signaling
https://read.qxmd.com/read/23337771/a-structural-perspective-of-the-mavs-regulatory-mechanism-on-the-mitochondrial-outer-membrane-using-bioluminescence-resonance-energy-transfer
#28
JOURNAL ARTICLE
Osamu Sasaki, Takuma Yoshizumi, Misa Kuboyama, Takeshi Ishihara, Emiko Suzuki, Shun-ichiro Kawabata, Takumi Koshiba
In most eukaryotic cells, mitochondria have various essential roles for proper cell function, such as energy production, and in mammals mitochondria also act as a platform for antiviral innate immunity. Mitochondrial-mediated antiviral immunity depends on the activation of the cytoplasmic retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs) signaling pathway, and on the participation of mitochondrial antiviral signaling (MAVS), which is localized on the mitochondrial outer membrane. After RNA virus infection, RLRs translocate to the mitochondrial surface to interact with MAVS, and the adaptor protein undergoes a conformational change that is essential for downstream signaling, although its structural features are poorly understood...
May 2013: Biochimica et Biophysica Acta
https://read.qxmd.com/read/22611464/microbe-specific-c3b-deposition-in-the-horseshoe-crab-complement-system-in-a-c2-factor-b-dependent-or-independent-manner
#29
JOURNAL ARTICLE
Keisuke Tagawa, Toyoki Yoshihara, Toshio Shibata, Kazuki Kitazaki, Yuichi Endo, Teizo Fujita, Takumi Koshiba, Shun-ichiro Kawabata
Complement C3 plays an essential role in the opsonization of pathogens in the mammalian complement system, whereas the molecular mechanism underlying C3 activation in invertebrates remains unknown. To understand the molecular mechanism of C3b deposition on microbes, we characterized two types of C2/factor B homologs (designated TtC2/Bf-1 and TtC2/Bf-2) identified from the horseshoe crab Tachypleus tridentatus. Although the domain architectures of TtC2/Bf-1 and TtC2/Bf-2 were identical to those of mammalian homologs, they contained five-repeated and seven-repeated complement control protein domains at their N-terminal regions, respectively...
2012: PloS One
https://read.qxmd.com/read/21528696/immunocompetent-molecules-and-their-response-network-in-horseshoe-crabs
#30
REVIEW
Shun-ichiro Kawabata
Horseshoe crab hemocyte selectively responds to bacterial lipopolysaccharides (LPS), which depends critically on the proteolytic activity of the LPS-responsive serine protease zymogen factor C. In response to stimulation by LPS, the hemocyte secretes several kinds of immunocompetent proteins. The coagulation cascade triggered by LPS or beta-1,3-D-glucans (BDG) results in the formation of coagulin fibrils that are subsequently stabilized by transglutaminase (TGase)-dependent cross-linking. Invading pathogens are recognized and agglutinated by lectins and then killed by antimicrobial peptides...
2010: Advances in Experimental Medicine and Biology
https://read.qxmd.com/read/21396342/tributyltin-binding-protein-type-1-a-lipocalin-prevents-inhibition-of-osteoblastic-activity-by-tributyltin-in-fish-scales
#31
JOURNAL ARTICLE
Hina Satone, Jae Man Lee, Yumi Oba, Takahiro Kusakabe, Eriko Akahoshi, Shizuho Miki, Nobuo Suzuki, Yuichi Sasayama, Mohamed Nassef, Yohei Shimasaki, Shun-Ichiro Kawabata, Tsuneo Honjo, Yuji Oshima
Tributyltin-binding protein type 1 (TBT-bp1) is a member of the lipocalin family of proteins which bind to small hydrophobic molecules. In this study, we expressed a recombinant TBT-bp1 (rTBT-bp1, ca. 35kDa) in a baculovirus expression system and purified the protein from the hemolymph of silkworm larvae injected with recombinant baculovirus. After incubation of a mixture of rTBT-bp1 and TBT and its fractionation by means of gel filtration chromatography, TBT was detected in the elution peak of rTBT-bp1, confirming the binding potential of rTBT-bp1 for TBT...
May 2011: Aquatic Toxicology
https://read.qxmd.com/read/21316390/report-on-the-22nd-annual-meeting-of-the-japanese-association-for-developmental-and-comparative-immunology-jadci-august-2-4-2010-nishijin-plaza-kyushu-university-fukuoka-local-organizer-shun-ichiro-kawabata-kyushu-university
#32
https://read.qxmd.com/read/21285412/mitochondrial-membrane-potential-is-required-for-mavs-mediated-antiviral-signaling
#33
JOURNAL ARTICLE
Takumi Koshiba, Kai Yasukawa, Yusuke Yanagi, Shun-ichiro Kawabata
Mitochondria, dynamic organelles that undergo cycles of fusion and fission, are the powerhouses of eukaryotic cells and are also involved in cellular innate antiviral immunity in mammals. Mitochondrial antiviral immunity depends on activation of the cytoplasmic retinoic acid-inducible gene I (RIG-I)-like receptor (RLR) signaling pathway and the participation of a mitochondrial outer membrane adaptor protein called MAVS (mitochondrial antiviral signaling). We found that cells that lack the ability to undergo mitochondrial fusion as a result of targeted deletion of both mitofusin 1 (Mfn1) and mitofusin 2 (Mfn2) exhibited impaired induction of interferons and proinflammatory cytokines in response to viral infection, resulting in increased viral replication...
2011: Science Signaling
https://read.qxmd.com/read/21036903/structure-function-analysis-of-the-yeast-mitochondrial-rho-gtpase-gem1p-implications-for-mitochondrial-inheritance
#34
JOURNAL ARTICLE
Takumi Koshiba, Holly A Holman, Kenji Kubara, Kai Yasukawa, Shun-ichiro Kawabata, Koji Okamoto, Jane MacFarlane, Janet M Shaw
Mitochondria undergo continuous cycles of homotypic fusion and fission, which play an important role in controlling organelle morphology, copy number, and mitochondrial DNA maintenance. Because mitochondria cannot be generated de novo, the motility and distribution of these organelles are essential for their inheritance by daughter cells during division. Mitochondrial Rho (Miro) GTPases are outer mitochondrial membrane proteins with two GTPase domains and two EF-hand motifs, which act as receptors to regulate mitochondrial motility and inheritance...
January 7, 2011: Journal of Biological Chemistry
https://read.qxmd.com/read/20976106/protein-crosslinking-by-transglutaminase-controls-cuticle-morphogenesis-in-drosophila
#35
JOURNAL ARTICLE
Toshio Shibata, Shigeru Ariki, Naoaki Shinzawa, Ryuta Miyaji, Haruka Suyama, Miyuki Sako, Nobuyuki Inomata, Takumi Koshiba, Hirotaka Kanuka, Shun-Ichiro Kawabata
Transglutaminase (TG) plays important and diverse roles in mammals, such as blood coagulation and formation of the skin barrier, by catalyzing protein crosslinking. In invertebrates, TG is known to be involved in immobilization of invading pathogens at sites of injury. Here we demonstrate that Drosophila TG is an important enzyme for cuticle morphogenesis. Although TG activity was undetectable before the second instar larval stage, it dramatically increased in the third instar larval stage. RNA interference (RNAi) of the TG gene caused a pupal semi-lethal phenotype and abnormal morphology...
October 18, 2010: PloS One
https://read.qxmd.com/read/20713082/peptidoglycan-activation-of-the-propo-system-without-a-peptidoglycan-receptor-protein-pgrp
#36
JOURNAL ARTICLE
Haipeng Liu, Chenglin Wu, Yasuyuki Matsuda, Shun-Ichiro Kawabata, Bok Luel Lee, Kenneth Söderhäll, Irene Söderhäll
Recognition of microbial polysaccharide by pattern recognition receptors triggers the prophenoloxidase (proPO) cascade, resulting in melanin synthesis and its deposition on the surface of invading pathogens. Several masquerade-like proteins and serine proteinase homologues have been shown to be involved in the proPO activation in insects. In this study, a novel serine proteinase homologue, Pl-SPH2, was found and isolated as a 30kDa protein from hemocytes of the freshwater crayfish, Pacifastacus leniusculus, by its binding property to a partially lysozyme digested or TCA-treated insoluble Lysine (Lys)-type peptidoglycan (PGN) and soluble polymeric Lys-type PGN...
January 2011: Developmental and Comparative Immunology
https://read.qxmd.com/read/20696274/purification-and-characterization-of-tributyltin-binding-protein-of-tiger-puffer-takifugu-rubripes
#37
JOURNAL ARTICLE
Yumi Oba, Akira Yamauchi, Yasuyuki Hashiguchi, Hina Satone, Shizuho Miki, Mohamed Nassef, Yohei Shimasaki, Takeshi Kitano, Miki Nakao, Shun-ichiro Kawabata, Tsuneo Honjo, Yuji Oshima
We successfully purified Trub.TBT-bpα, a tributyltin (TBT) binding protein (bp) of the tiger puffer, Takifugu rubripes. Tiger puffer was injected intraperitoneally with TBT (1.0mg/kg body weight) and Trub.TBT-bpα was purified from serum by ammonium sulfate fractionation, gel filtration chromatography and polyacrylamide gel electrophoresis. Gel electrophoresis revealed that the Trub.TBT-bpα has a molecular mass of approximately 48.5kDa and contains at least 40% N-glycan. The deduced 212 amino acid sequence of the protein showed the highest identity (41%, 212 amino acid overlap and E-value: 9e-42) with TBT-binding protein type 1 (TBT-bp1) of Paralichthys olivaceus (Japanese flounder)...
January 2011: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://read.qxmd.com/read/20541942/proteolytic-cascades-and-their-involvement-in-invertebrate-immunity
#38
REVIEW
Lage Cerenius, Shun-ichiro Kawabata, Bok Luel Lee, Masaru Nonaka, Kenneth Söderhäll
Bacteria and other potential pathogens are cleared rapidly from the body fluids of invertebrates by the immediate response of the innate immune system. Proteolytic cascades, following their initiation by pattern recognition proteins, control several such reactions, notably coagulation, melanisation, activation of the Toll receptor and complement-like reactions. However, there is considerable variation among invertebrates and these cascades, although widespread, are not present in all phyla. In recent years, significant progress has been made in identifying and characterizing these cascades in insects...
October 2010: Trends in Biochemical Sciences
https://read.qxmd.com/read/20406733/sadaaki-iwanaga-discovery-of-the-lipopolysaccharide-and-beta-1-3-d-glucan-mediated-proteolytic-cascade-and-unique-proteins-in-invertebrate-immunity
#39
REVIEW
Shun-ichiro Kawabata, Tatsushi Muta
Horseshoe crab haemolymph contains a single type of cells, granular haemocytes, which are extremely sensitive to bacterial lipopolysaccharides (LPS) and lead to haemolymph coagulation. Sadaaki Iwanaga isolated protease zymogens from the haemocytes and reconstituted LPS and beta-1,3-d-glucans-mediated haemolymph coagulation. This led to the first discovery of a proteolytic cascade triggered by pathogen-associated molecular patterns, an important milestone for studies on invertebrate innate immunity. Moreover, he separated components derived from haemocyte granules and haemolymph plasma, and consequently identified unique defense molecules, such as lectins, serpins, cystatins, antimicrobial substances and substrates for transglutaminase...
May 2010: Journal of Biochemistry
https://read.qxmd.com/read/19710471/factor-g-utilizes-a-carbohydrate-binding-cleft-that-is-conserved-between-horseshoe-crab-and-bacteria-for-the-recognition-of-beta-1-3-d-glucans
#40
JOURNAL ARTICLE
Yuki Ueda, Shuhei Ohwada, Yoshito Abe, Toshio Shibata, Manabu Iijima, Yukiko Yoshimitsu, Takumi Koshiba, Munehiro Nakata, Tadashi Ueda, Shun-ichiro Kawabata
In the horseshoe crab, the recognition of beta-1,3-D-glucans by factor G triggers hemolymph coagulation. Factor G contains a domain of two tandem xylanase Z-like modules (Z1-Z2), each of which recognizes beta-1,3-D-glucans. To gain an insight into the recognition of beta-1,3-D-glucans from a structural view point, recombinants of Z1-Z2, the C-terminal module Z2, Z2 with a Cys to Ala substitution (Z2A), and its tandem repeat Z2A-Z2A were characterized. Z2 and Z1-Z2, but not Z2A and Z2A-Z2A, formed insoluble aggregates at higher concentrations more than approximately 30 and 3 microM, respectively...
September 15, 2009: Journal of Immunology
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