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Jiyeun Kate Kim

Jiyeun Kate Kim, Ho Am Jang, Min Seon Kim, Jae Hyun Cho, Junbeom Lee, Flaviana Di Lorenzo, Luisa Sturiale, Alba Silipo, Antonio Molinaro, Bok Luel Lee
Lipopolysaccharide, the outer cell-wall component of Gram-negative bacteria, has been shown to be important for symbiotic associations. We recently reported that the lipopolysaccharide O-antigen of Burkholderia enhances the initial colonization of the midgut of the bean bug, Riptortus pedestris However, the midgut-colonizing Burkholderia symbionts lack the O-antigen but display the core oligosaccharide on the cell surface. In this study, we investigated the role of the core oligosaccharide, which directly interacts with the host midgut, in the Riptortus-Burkholderia symbiosis...
November 24, 2017: Journal of Biological Chemistry
Allison Case, Angela Desmond, Daniel Lopes, Kelly Dye, Kelly Mapes, Stephen Ruback, Iliodora Pop, Jiyeun Kate Kim, Pavitra Chakravarty, Joan E Smallshaw, Laurentiu M Pop, Ellen S Vitetta
We demonstrate that a peptoid composed of five monomers and attached via a maleimide linker to a carrier protein elicits anti-peptoid, anti-linker and anti-carrier antibodies in rabbits. Specific anti-peptoid antibodies were affinity purified and used to reproducibly retrieve three specific peptoid-coupled beads from 20,000 irrelevant peptoid-beads using magnetic screening.
August 2016: Journal of Vaccines & Vaccination
Jiyeun Kate Kim, Ha Young Park, Bok Luel Lee
Riptortus pedestris harboring Burkholderia symbiont is a useful symbiosis model to study the molecular interactions between insects and bacteria. We recently reported that the lipopolysaccharide O-antigen is absent in the Burkholderia symbionts isolated from Riptortus guts. Here, we investigated the symbiotic role of O-antigen comprehensively in the Riptortus-Burkholderia model. Firstly, Burkholderia mutant strains deficient of O-antigen biosynthesis genes were generated and confirmed for their different patterns of the lipopolysaccharide by electrophoretic analysis...
July 2016: Developmental and Comparative Immunology
Jiyeun Kate Kim, Jun Beom Lee, Ho Am Jang, Yeon Soo Han, Takema Fukatsu, Bok Luel Lee
Valuable insect models have tremendously contributed to our understanding of innate immunity and symbiosis. Bean bug, Riptortus pedestris, is a useful insect symbiosis model due to harboring cultivable monospecific gut symbiont, genus Burkholderia. Bean bug is a hemimetabolous insect whose immunity is not well-understood. However, we recently identified three major antimicrobial peptides of Riptortus and examined the relationship between gut symbiosis and host immunity. We found that the presence of Burkholderia gut symbiont positively affects Riptortus immunity...
November 2016: Developmental and Comparative Immunology
Jun Beom Lee, Jin Hee Byeon, Ho Am Jang, Jiyeun Kate Kim, Jin Wook Yoo, Yoshitomo Kikuchi, Bok Luel Lee
We generated a Burkholderia mutant, which is deficient of an N-acetylmuramyl-l-alanine amidase, AmiC, involved in peptidoglycan degradation. When non-motile ΔamiC mutant Burkholderia cells harboring chain form were orally administered to Riptortus insects, ΔamiC mutant cells were unable to establish symbiotic association. But, ΔamiC mutant complemented with amiC gene restored in vivo symbiotic association. ΔamiC mutant cultured in minimal medium restored their motility with single-celled morphology. When ΔamiC mutant cells harboring single-celled morphology were administered to the host insect, this mutant established normal symbiotic association, suggesting that bacterial motility is essential for the successful symbiosis between host insect and Burkholderia symbiont...
September 14, 2015: FEBS Letters
Jiyeun Kate Kim, Jun Beom Lee, Ye Rang Huh, Ho Am Jang, Chan-Hee Kim, Jin Wook Yoo, Bok Luel Lee
The relation between gut symbiosis and immunity has been reported in various animal model studies. Here, we corroborate the effect of gut symbiont to host immunity using the bean bug model. The bean bug, Riptortus pedestris, is a useful gut symbiosis model due to the monospecific gut symbiont, genus Burkholderia. To examine the effect of gut symbiosis to host immunity, we generated the gut symbiont-harboring (symbiotic) insect line and the gut symbiont-lacking (aposymbiotic) insect line. Upon bacterial challenges, the symbiotic Riptortus exhibited better survival than aposymbiotic Riptortus...
November 2015: Developmental and Comparative Immunology
Jin Hee Byeon, Eun Sil Seo, Jun Beom Lee, Min Ja Lee, Jiyeun Kate Kim, Jin Wook Yoo, Yunjin Jung, Bok Luel Lee
Because gut symbiotic bacteria affect host biology, host insects are expected to evolve some mechanisms for regulating symbiont population. The bean bug, Riptortus pedestris, harbors the Burkholderia genus as a gut symbiont in the midgut organ, designated as the M4 region. Recently, we demonstrated that the lysate of M4B, the region adjacent to M4, harbors potent antibacterial activity against symbiotic Burkholderia but not to cultured Burkholderia. However, the bona fide substance responsible for observed antibacterial activity was not identified in the previous study...
November 2015: Developmental and Comparative Immunology
Jiyeun Kate Kim, Dae Woo Son, Chan-Hee Kim, Jae Hyun Cho, Roberta Marchetti, Alba Silipo, Luisa Sturiale, Ha Young Park, Ye Rang Huh, Hiroshi Nakayama, Takema Fukatsu, Antonio Molinaro, Bok Luel Lee
The molecular characterization of symbionts is pivotal for understanding the cross-talk between symbionts and hosts. In addition to valuable knowledge obtained from symbiont genomic studies, the biochemical characterization of symbionts is important to fully understand symbiotic interactions. The bean bug (Riptortus pedestris) has been recognized as a useful experimental insect gut symbiosis model system because of its cultivatable Burkholderia symbionts. This system is greatly advantageous because it allows the acquisition of a large quantity of homogeneous symbionts from the host midgut...
August 21, 2015: Journal of Biological Chemistry
Jiyeun Kate Kim, Bok Luel Lee
Symbiotic bacteria are common in insects and intimately affect the various aspects of insect host biology. In a number of insect symbiosis models, it has been possible to elucidate the effects of the symbiont on host biology, whereas there is a limited understanding of the impact of the association on the bacterial symbiont, mainly due to the difficulty of cultivating insect symbionts in vitro. Furthermore, the molecular features that determine the establishment and persistence of the symbionts in their host (i...
January 2015: Archives of Insect Biochemistry and Physiology
Jiyeun Kate Kim, Jeong Yun Kwon, Soo Kyoung Kim, Sang Heum Han, Yeo Jin Won, Joon Hee Lee, Chan-Hee Kim, Takema Fukatsu, Bok Luel Lee
The Riptortus-Burkholderia symbiotic system is an experimental model system for studying the molecular mechanisms of an insect-microbe gut symbiosis. When the symbiotic midgut of Riptortus pedestris was investigated by light and transmission electron microscopy, the lumens of the midgut crypts that harbor colonizing Burkholderia symbionts were occupied by an extracellular matrix consisting of polysaccharides. This observation prompted us to search for symbiont genes involved in the induction of biofilm formation and to examine whether the biofilms are necessary for the symbiont to establish a successful symbiotic association with the host...
July 2014: Applied and Environmental Microbiology
Jiyeun Kate Kim, Sang Heum Han, Chan-Hee Kim, Yong Hun Jo, Ryo Futahashi, Yoshitomo Kikuchi, Takema Fukatsu, Bok Luel Lee
The majority of insects possess symbiotic bacteria. Since symbiont titers can affect host phenotypes of biological importance, host insects are expected to evolve some mechanisms for regulating symbiont population. Here we report that, in the Riptortus-Burkholderia gut symbiosis, titers of the beneficial symbiont transiently decrease at the pre-molt stages in host development. This molting-associated suppression of the symbiont population is coincident with the increase of antimicrobial activity in the symbiotic midgut, which is observed in both symbiotic and aposymbiotic insects...
March 2014: Developmental and Comparative Immunology
Jiyeun Kate Kim, Ho Am Jang, Yeo Jin Won, Yoshitomo Kikuchi, Sang Heum Han, Chan-Hee Kim, Naruo Nikoh, Takema Fukatsu, Bok Luel Lee
The Riptortus-Burkholderia symbiotic system represents a promising experimental model to study the molecular mechanisms involved in insect-bacterium symbiosis due to the availability of genetically manipulated Burkholderia symbiont. Using transposon mutagenesis screening, we found a symbiosis-deficient mutant that was able to colonize the host insect but failed to induce normal development of host's symbiotic organ. The disrupted gene was identified as purL involved in purine biosynthesis. In vitro growth impairment of the purL mutant and its growth dependency on adenine and adenosine confirmed the functional disruption of the purine synthesis gene...
March 2014: ISME Journal
Jiyeun Kate Kim, Na Hyang Kim, Ho Am Jang, Yoshitomo Kikuchi, Chan-Hee Kim, Takema Fukatsu, Bok Luel Lee
Many insects possess symbiotic bacteria that affect the biology of the host. The level of the symbiont population in the host is a pivotal factor that modulates the biological outcome of the symbiotic association. Hence, the symbiont population should be maintained at a proper level by the host's control mechanisms. Several mechanisms for controlling intracellular symbionts of insects have been reported, while mechanisms for controlling extracellular gut symbionts of insects are poorly understood. The bean bug Riptortus pedestris harbors a betaproteobacterial extracellular symbiont of the genus Burkholderia in the midgut symbiotic organ designated the M4 region...
December 2013: Applied and Environmental Microbiology
Jiyeun Kate Kim, Yeo Jin Won, Naruo Nikoh, Hiroshi Nakayama, Sang Heum Han, Yoshitomo Kikuchi, Young Ha Rhee, Ha Young Park, Jeong Yun Kwon, Kenji Kurokawa, Naoshi Dohmae, Takema Fukatsu, Bok Luel Lee
Many bacteria accumulate granules of polyhydroxyalkanoate (PHA) within their cells, which confer resistance to nutritional depletion and other environmental stresses. Here, we report an unexpected involvement of the bacterial endocellular storage polymer, PHA, in an insect-bacterium symbiotic association. The bean bug Riptortus pedestris harbors a beneficial and specific gut symbiont of the β-proteobacterial genus Burkholderia, which is orally acquired by host nymphs from the environment every generation and easily cultivable and genetically manipulatable...
June 25, 2013: Proceedings of the National Academy of Sciences of the United States of America
Jiyeun Kate Kim, Ho Jin Lee, Yoshitomo Kikuchi, Wataru Kitagawa, Naruo Nikoh, Takema Fukatsu, Bok Luel Lee
To establish a host-bacterium symbiotic association, a number of factors involved in symbiosis must operate in a coordinated manner. In insects, bacterial factors for symbiosis have been poorly characterized at the molecular and biochemical levels, since many symbionts have not yet been cultured or are as yet genetically intractable. Recently, the symbiotic association between a stinkbug, Riptortus pedestris, and its beneficial gut bacterium, Burkholderia sp., has emerged as a promising experimental model system, providing opportunities to study insect symbiosis using genetically manipulated symbiotic bacteria...
August 2013: Applied and Environmental Microbiology
Jiyeun Kate Kim, Yi Xu, Xuejun Xu, Douglas R Keene, Sivashankarappa Gurusiddappa, Xiaowen Liang, Kishore K Wary, Magnus Höök
Previously identified high affinity integrin-binding motifs in collagens, GFOGER and GLOGER, are not present in type III collagen. Here, we first characterized the binding of recombinant I domains from integrins alpha(1) and alpha(2) (alpha(1)I and alpha(2)I) to fibrillar collagen types I-III and showed that each I domain bound to the three types of collagens with similar affinities. Using rotary shadowing followed by electron microscopy, we identified a high affinity binding region in human type III collagen recognized by alpha(1)I and alpha(2)I...
September 16, 2005: Journal of Biological Chemistry
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