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Kenneth Soderhall

Kingkamon Junkunlo, Kenneth Söderhäll, Irene Söderhäll
Hematopoietic progenitor cells in crustaceans are organized in lobule-like structures surrounded by different types of cells and extracellular matrix (ECM) proteins in a Hematopoietic tissue (HPT). Here we show that the clotting protein (CP) is part of the ECM in HPT and is secreted during HPT cell culture. The formation of a filamentous network of CP was observed in HPT cell culture. A high amount of CP protein was detected at the surfaces of undifferentiated cells (round-shaped) compared with migrating cells (spindle shaped)...
September 21, 2017: Developmental and Comparative Immunology
Kingkamon Junkunlo, Kenneth Söderhäll, Chadanat Noonin, Irene Söderhäll
The platelet-derived growth factor (PDGF) receptor, a tyrosine kinase (TK) receptor whose ligand is PDGF, is crucial in the transduction of extracellular signals into cells and mediates numerous processes, such as cell proliferation, differentiation, survival, and migration. We demonstrate the important roles of a receptor TK related to the PDGF/VEGF family protein (PVR) in controlling hematopoietic progenitor cell migration by affecting extracellular transglutaminase (TGase) activity. Pl_PVR1, GenBank accession No...
October 15, 2017: Stem Cells and Development
Enen Guo, Gül Gizem Korkut, Phattarunda Jaree, Irene Söderhäll, Kenneth Söderhäll
Serine proteases are involved in many critical physiological processes including virus spread and replication. In the present study, we identified a new clip-domain serine protease (PlcSP) in the crayfish Pacifastacus leniusculus hemocytes, which can interact with the White Spot Syndrome Virus (WSSV) envelope protein VP28. It was characterized by a classic clip domain with six strictly conserved Cys residues, and contained the conserved His-Asp-Ser (H-D-S) motif in the catalytic domain. Furthermore, signal peptide prediction revealed that it has a 16-residue secretion signal peptide...
July 10, 2017: Fish & Shellfish Immunology
Ratchanok Sirikharin, Kingkamon Junkunlo, Kenneth Söderhäll, Irene Söderhäll
Transglutaminase (TGase) has been implicated in maintaining the undifferentiated stage of hematopoietic stem cells (HSC) in the crayfish Pacifastacus leniusculus. TGase activity has been reported to be regulated by astakine1, an essential crayfish cytokine for inducing new hemocyte synthesis in hematopoietic tissue (HPT). Here, the role of astakine1 in TGase activity regulation and clotting protein (CP) cross-linking was characterized. A reduction in TGase activity was observed by the addition of purified astakine1 in vitro for both endogenous crayfish TGase and a commercial purified guinea pig liver TGase...
November 2017: Developmental and Comparative Immunology
Lage Cerenius, Kenneth Söderhäll
Freshwater crayfish is an important commodity as well as a successful model for studies on crustacean immunity. Due to the ease with which they are kept and the available methods for hemocyte separation and culture they have proven to be very useful. Here, recent progress regarding pattern recognition, immune effector production and antiviral mechanisms are discussed. Several cases of functional resemblance between vertebrate complement and the crayfish immune reactions are highlighted.
March 2018: Developmental and Comparative Immunology
Miti Jearaphunt, Chadanat Noonin, Pikul Jiravanichpaisal, Seiko Nakamura, Anchalee Tassanakajon, Irene Söderhäll, Kenneth Söderhäll
[This corrects the article DOI: 10.1371/journal.ppat.1004059.].
May 2016: PLoS Pathogens
Apiruck Watthanasurorot, Pikul Jiravanichpaisal, Haipeng Liu, Irene Söderhäll, Kenneth Söderhäll
No abstract text is available yet for this article.
2016: PLoS Pathogens
Kenneth Söderhäll
No abstract text is available yet for this article.
February 2016: Developmental and Comparative Immunology
Wipasiri Soonthornchai, Sage Chaiyapechara, Padermsak Jarayabhand, Kenneth Söderhäll, Pikul Jiravanichpaisal
Several species of Vibrio are the causative agent of gastroenteritis in humans. In aquaculture, Vibrio harveyi (Vh) and V. parahaemolyticus (Vp) have long been considered as shrimp pathogens in freshwater, brackish and marine environments. Here we show by using scanning electron microscopy (SEM) that Penaeus monodon orally inoculated with each of these two pathogens via an Artemia diet had numerous bacteria attached randomly across the stomach surface, in single and in large biofilm-like clusters 6 h post-infection...
2015: PloS One
Lulu Kong, Anrui Lu, Jingmin Guan, Bing Yang, Muwang Li, Julián F Hillyer, Nalini Ramarao, Kenneth Söderhäll, Chaoliang Liu, Erjun Ling
Thermolysin, a metallopeptidase secreted by pathogenic microbes, is concluded as an important virulence factor due to cleaving purified host proteins in vitro. Using the silkworm Bombyx mori as a model system, we found that thermolysin injection into larvae induces the destruction of the coagulation response and the activation of hemolymph melanization, which results in larval death. Thermolysin triggers the rapid degradation of insect and mammalian plasma proteins at a level that is considerably greater than expected in vitro and/or in vivo...
January 2015: Archives of Insect Biochemistry and Physiology
Apiruck Watthanasurorot, Enen Guo, Sirinit Tharntada, Chu-Fang Lo, Kenneth Söderhäll, Irene Söderhäll
We have previously shown that multifunctional calreticulin (CRT), which resides in the endoplasmic reticulum (ER) and is involved in ER-associated protein processing, responds to infection with white spot syndrome virus (WSSV) by increasing mRNA and protein expression and by forming a complex with gC1qR and thereby delaying apoptosis. Here, we show that CRT can directly interact with WSSV structural proteins, including VP15 and VP28, during an early stage of virus infection. The binding of VP28 with CRT does not promote WSSV entry, and CRT-VP15 interaction was detected in the viral genome in virally infected host cells and thus may have an effect on WSSV replication...
July 2014: Journal of Virology
Miti Jearaphunt, Chadanat Noonin, Pikul Jiravanichpaisal, Seiko Nakamura, Anchalee Tassanakajon, Irene Söderhäll, Kenneth Söderhäll
Invertebrates rely on innate immunity to respond to the entry of foreign microorganisms. One of the important innate immune responses in arthropods is the activation of prophenoloxidase (proPO) by a proteolytic cascade finalized by the proPO-activating enzyme (ppA), which leads to melanization and the elimination of pathogens. Proteolytic cascades play a crucial role in innate immune reactions because they can be triggered more quickly than immune responses that require altered gene expression. Caspases are intracellular proteases involved in tightly regulated limited proteolysis of downstream processes and are also involved in inflammatory responses to infections for example by activation of interleukin 1ß...
April 2014: PLoS Pathogens
Anrui Lu, Xuquan Li, Julián F Hillyer, Brenda T Beerntsen, Kenneth Söderhäll, Erjun Ling
Insect prophenoloxidase (PPO) is an essential innate immunity protein to induce pathogen into melanization. In Bombyx mori, pro-phenoloxidase-activating enzyme (PPAE) can directly cleave and activate PPO. However, PPO in Manduca sexta cannot be cleaved into active phenoloxidase (PO) by serine proteases unless cofactors are involved, which indicates that PPO activation is complicated. Here we use recombinant Drosophila melanogaster prophenoloxidase 1 (rPPO1) to study the mechanism of PPO activation induced by a typical serine protease, α-chymotrypsin...
July 2014: Biochimie
Lage Cerenius, Kenneth Söderhäll
Recently it has become evident that invertebrates may mount a highly variable immune response that is dependent on which pathogen is involved. The molecular mechanisms behind this diversity are beginning to be unravelled and in several invertebrate taxa immune proteins exhibiting a broad range of diversity have been found. In some cases, evidence has been gathered suggesting that this molecular diversity translates into the ability of an affected invertebrate to mount a defence that is specifically aimed at a particular pathogen...
December 1, 2013: Journal of Experimental Biology
Netnapa Saelee, Chadanat Noonin, Benjamas Nupan, Kingkamon Junkunlo, Amornrat Phongdara, Xionghui Lin, Kenneth Söderhäll, Irene Söderhäll
Thymosin proteins are well known for their actin-binding activity. Thymosin beta 4 (Tβ4) has been associated with biological activities in tissue repair and cell migration via interaction with ATP-synthase in vertebrates, while the information of similar thymosin functions in invertebrates is limited. We have shown previously that ATP-synthase is present on the surface of crayfish hematopoietic tissue (HPT) cells, and that astakine 1 (Ast1, an invertebrate cytokine) was found to interact with this β-subunit of ATP synthase...
2013: PloS One
Apiruck Watthanasurorot, Netnapa Saelee, Amornrat Phongdara, Sittiruk Roytrakul, Pikul Jiravanichpaisal, Kenneth Söderhäll, Irene Söderhäll
Daily, circadian rhythms influence essentially all living organisms and affect many physiological processes from sleep and nutrition to immunity. This ability to respond to environmental daily rhythms has been conserved along evolution, and it is found among species from bacteria to mammals. The hematopoietic process of the crayfish Pacifastacus leniusculus is under circadian control and is tightly regulated by astakines, a new family of cytokines sharing a prokineticin (PROK) domain. The expression of AST1 and AST2 are light-dependent, and this suggests an evolutionarily conserved function for PROK domain proteins in mediating circadian rhythms...
March 2013: PLoS Genetics
Apiruck Watthanasurorot, Pikul Jiravanichpaisal, Kenneth Söderhäll, Irene Söderhäll
The crossroad between cell death and proliferation is a general target for viral infections because viruses need to obstruct apoptosis to use cells for their own replication. Inducing immunogenic cell death in proliferating cells is also an important aim of anticancer chemotherapy. The C1q-binding proteins calreticulin (CRT) and gC1qR are highly conserved ubiquitous proteins, which are putative targets for viral manipulation and are associated with cancer. Here we show that these proteins form a complex in the cytoplasm as a response to viral infection resulting in apoptosis prevention...
April 2013: Journal of Molecular Cell Biology
Yupu Diao, Anrui Lu, Bing Yang, Wenli Hu, Qing Peng, Qing-Zhi Ling, Brenda T Beerntsen, Kenneth Söderhäll, Erjun Ling
In insects, hemocytes are considered as the only source of plasma prophenoloxidase (PPO). PPO also exists in the hemocytes of the hematopoietic organ that is connected to the wing disc of Bombyx mori. It is unknown whether there are other cells or tissues that can produce PPO and release it into the hemolymph besides circulating hemocytes. In this study, we use the silkworm as a model to explore this possibility. Through tissue staining and biochemical assays, we found that wing discs contain PPO that can be released into the culture medium in vitro...
2012: PloS One
Chadanat Noonin, Xionghui Lin, Pikul Jiravanichpaisal, Kenneth Söderhäll, Irene Söderhäll
During evolution, the innate and adaptive immune systems were developed to protect organisms from non-self substances. The innate immune system is phylogenetically more ancient and is present in most multicellular organisms, whereas adaptive responses are restricted to vertebrates. Arthropods lack the blood cells of the lymphoid lineage and oxygen-carrying erythrocytes, making them suitable model animals for studying the regulation of the blood cells of the innate immune system. Many crustaceans have a long life span and need to continuously synthesize blood cells, in contrast to many insects...
November 20, 2012: Stem Cells and Development
Chenglin Wu, Walaiporn Charoensapsri, Seiko Nakamura, Anchalee Tassanakajon, Irene Söderhäll, Kenneth Söderhäll
An important characteristic of the innate immune systems of crayfish and other arthropods is the activation of a serine proteinase cascade in the hemolymph, which results in the activation of prophenoloxidase and subsequently leading to the formation of toxic quinones and melanin. Although no true complement homologues have been detected in crayfish or crustaceans, several proteins with similarities to vertebrate pattern recognition receptors (PRRs), which are involved in the lectin pathway of complement activation in vertebrates, are present...
February 2013: Immunobiology
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