keyword
MENU ▼
Read by QxMD icon Read
search

fibronectin

keyword
https://www.readbyqxmd.com/read/28806534/knockdown-of-snail-inhibits-epithelial-mesenchymal-transition-of-human-laryngeal-squamous-cell-carcinoma-hep-2-cells-through-vdr-signaling-pathway
#1
Xue Zhao, Dan Yu, Jingpu Yang, Kai Xue, Yan Liu, Chunshun Jin
It has been well-documented that Snail plays a decisive role in various tumors. However, the direct effect of Snail on laryngeal squamous cell carcinoma (LSCC) has not been elaborated. In this study, we firstly detected the expression of Snail in 14 samples of patients with LSCC and found that its content was high in cancer tissues compared with adjacent tissues. Then we established LSCC Hep-2 cells with Snail silencing and validated the knockdown efficiency by western blotting and real-time PCR. Results showed that silencing of Snail significantly inhibited the ability of adhesion, migration, and invasion of Hep-2 cells...
August 14, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28803967/igdb-2-an-ig-fniii-protein-binds-the-ion-channel-lgc-34-and-controls-sensory-compartment-morphogenesis-in-c-elegans
#2
Wendy Wang, Elliot A Perens, Grigorios Oikonomou, Yun Lu, Shai Shaham
Sensory organ glia surround neuronal receptive endings (NREs), forming a specialized compartment important for neuronal activity, and reminiscent of glia-ensheathed synapses in the central nervous system. We previously showed that DAF-6, a Patched-related protein, is required in glia of the C. elegans amphid sensory organ to restrict sensory compartment size. LIT-1, a Nemo-like kinase, and SNX-1, a retromer component, antagonize DAF-6 and promote compartment expansion. To further explore the machinery underlying compartment size control, we sought genes whose inactivation restores normal compartment size to daf-6 mutants...
August 10, 2017: Developmental Biology
https://www.readbyqxmd.com/read/28803516/well-defined-protein-immobilization-on-photo-responsive-phosphorylcholine-polymer-surfaces
#3
Masako Tanaka, Shugo Kawai, Yasuhiko Iwasaki
In this study, we propose a new polymer substrate that is able to covalently couple intended proteins and reduce nonspecific protein fouling. Poly[2-methacryloyloxyethyl phosphorylcholine (MPC)-ran-N-methacryloyl-(L)-tyrosinemethylester (MAT)] [P(MPC/MAT)] was synthesized by free-radical polymerization. The photooxidation of the MAT unit in the copolymer was observed under ultraviolet (UV) light at 254 nm. P(MPC/MAT) was spin-coated on silicon (Si) and gold substrates. Without UV irradiation of the polymer-coated surface, P(MPC/MAT) physisorbed on the substrates, and the thickness of the polymer layer was less than 10 nm, regardless of the polymer concentration in the coating solution...
August 14, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28803268/proteomic-profiling-of-the-dystrophin-complex-and-membrane-fraction-from-dystrophic-mdx-muscle-reveals-decreases-in-the-cytolinker-desmoglein-and-increases-in-the-extracellular-matrix-stabilizers-biglycan-and-fibronectin
#4
Sandra Murphy, Heinrich Brinkmeier, Mirjam Krautwald, Michael Henry, Paula Meleady, Kay Ohlendieck
The almost complete loss of the membrane cytoskeletal protein dystrophin and concomitant drastic reduction in dystrophin-associated glycoproteins are the underlying mechanisms of the highly progressive neuromuscular disorder Duchenne muscular dystrophy. In order to identify new potential binding partners of dystrophin or proteins in close proximity to the sarcolemmal dystrophin complex, proteomic profiling of the isolated dystrophin-glycoprotein complex was carried out. Subcellular membrane fractionation and detergent solubilisation, in combination with ion exchange, lectin chromatography and density gradient ultracentrifugation, was performed to isolate a dystrophin complex-enriched fraction...
August 12, 2017: Journal of Muscle Research and Cell Motility
https://www.readbyqxmd.com/read/28801552/gene-location-expression-and-function-of-fndc5-in-meishan-pigs
#5
Chunbo Cai, Gaojun Xiao, Lili Qian, Shengwang Jiang, Biao Li, Shanshan Xie, Ting Gao, Xiaorong An, Wentao Cui, Kui Li
Irisin is a new muscular regulatory factor that is generated by the cleavage of its precursor protein fibronectin type III domain-containing protein 5 (FNDC5). Irisin promotes fat consumption due to its stimulatory role in the browning of the adipocytes in mice. Currently, there is no report on FNDC5 functions in pigs as model animals. In this study, we investigated the expression patterns and functions of FNDC5 in Meishan pigs. Our results showed that FNDC5 gene in Meishan pigs contains five transcripts, all of which can be translated into functional intact irisin proteins...
August 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28801205/recombinant-laminin-fragments-endowed-with-collagen-binding-activity-a-tool-for-conferring-laminin-like-cell-adhesive-activity-to-collagen-matrices
#6
Ryoko Sato-Nishiuchi, Shaoliang Li, Fumi Ebisu, Kiyotoshi Sekiguchi
Laminins are major components of basement membranes that sustain a wide variety of stem cells. Among 15 laminin isoforms, laminin-511 and its E8 fragment (LM511E8) have been shown to strongly promote the adhesion and proliferation of human pluripotent stem cells. The aim of this study was to endow the cell-adhesive activity of laminin-511 on collagen matrices, thereby fabricating collagen-based culture scaffolds for stem cells with defined composition. To achieve this goal, we utilized the collagen-binding domain (CBD) of fibronectin to immobilize LM511E8 on collagen matrices...
August 8, 2017: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/28800587/highly-efficient-gene-inactivation-by-adenoviral-crispr-cas9-in-human-primary-cells
#7
Olaf Voets, Frans Tielen, Edo Elstak, Julian Benschop, Max Grimbergen, Jan Stallen, Richard Janssen, Andre van Marle, Christian Essrich
Phenotypic assays using human primary cells are highly valuable tools for target discovery and validation in drug discovery. Expression knockdown (KD) of such targets in these assays allows the investigation of their role in models of disease processes. Therefore, efficient and fast modes of protein KD in phenotypic assays are required. The CRISPR/Cas9 system has been shown to be a versatile and efficient means of gene inactivation in immortalized cell lines. Here we describe the use of adenoviral (AdV) CRISPR/Cas9 vectors for efficient gene inactivation in two human primary cell types, normal human lung fibroblasts and human bronchial epithelial cells...
2017: PloS One
https://www.readbyqxmd.com/read/28800110/response-of-dermal-fibroblasts-to-biochemical-and-physical-cues-in-aligned-polycaprolactone-silk-fibroin-nanofiber-scaffolds-for-application-in-tendon-tissue-engineering
#8
Chih-Hao Chen, Shih-Hsien Chen, Chang-Yi Kuo, Meng-Lun Li, Jyh-Ping Chen
Silk fibroin (SF) and fiber alignment were introduced into polycaprolactone (PCL)-based electrospun nanofibers as chemical and physical cues for tendon tissue engineering applications. The physicochemical properties of random PCL (RP) nanofibers, random PCL/SF (RPSF) nanofibers and aligned PCL/SF (APSF) nanofibers were characterized for fiber orientation and SF blending effects. An in vitro cell culture with rabbit dermal fibroblasts (RDFBs) on nanofibers indicated that SF promotes cell proliferation to a higher extent than fiber alignment...
August 11, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28798804/sinomenine-hydrochloride-attenuates-renal-fibrosis-by-inhibiting-excessive-autophagy-induced-by-adriamycin-an-experimental-study
#9
Ming-Ming Zhao, Bin Yang, Qiu Zhang, Jin-Hu Wang, Jin-Ning Zhao, Yu Zhang
The objective of this study is to investigate if sinomenine hydrochloride (SIN-HCl) could be effective against adriamycin-induced renal fibrosis by regulating autophagy in a rat model. Forty male Sprague-Dawley (SD) rats were randomly divided into control group, model group, telmisartan group, and SIN-HCl group; rat model was induced by adriamycin; all rats were given intragastric administration for 6 weeks. Urine was collected from rats in metabolic cages to determine 24 h protein level. This was done after intragastric administration for the first two weeks and then once for every two weeks...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28794269/the-fc%C3%AE%C2%B5ri-signaling-cascade-and-integrin-trafficking-converge-at-patterned-ligand-surfaces
#10
Devin L Wakefield, David Holowka, Barbara Baird
We examined the spatial targeting of early and downstream signaling mediated by the IgE receptor (FcεRI) in RBL mast cells utilizing surface-patterned 2,4 dinitrophenyl (DNP) ligands. Micron-sized features of DNP are presented as densely immobilized conjugates of bovine serum albumin (DNP-BSA) or mobile in a supported lipid bilayer (DNP-SLB). Although soluble anti-DNP IgE binds uniformly across features for both pattern types, IgE bound to FcεRI on cells shows distinctive distributions: uniform for DNP-SLB and edge-concentrated for DNP-BSA...
August 9, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28794036/persistent-tgev-infection-enhances-etec-k88-adhesion-by-promoting-epithelial-mesenchymal-transition-in-intestinal-epithelial-cells
#11
Lu Xia, Lei Dai, Qinghua Yu, Qian Yang
Transmissible gastroenteritis virus (TGEV) is a coronavirus, characterized by diarrhea, high morbidity, and the mortality is 100% in piglets less than 2 weeks old. Pigs infected with TGEV are often suffer secondary infection with other pathogens, which aggravates the severity of diarrhea, but the mechanisms remain unknown. Here, we hypothesized that persistent TGEV infection stimulates the epithelial-mesenchymal transition (EMT), thereby generating cells that more easily adhere to enterotoxigenic Escherichia coli (ETEC)...
August 9, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28792486/trmfo-a-fibronectin-binding-adhesin-of-mycoplasma-bovis
#12
Yongpeng Guo, Hongmei Zhu, Jiayao Wang, Jing Huang, Farhan Anwar Khan, Jingjing Zhang, Aizhen Guo, Xi Chen
Mycoplasma bovis is an important pathogenic mycoplasma, causing the cattle industry serious economic losses. Adhesion is a crucial step in the mycoplasmas' infection and colonization process; fibronectin (Fn), an extracellular matrix glycoprotein, is a molecular bridge between the bacterial adhesins and host cell receptors. The present study was designed to characterize the Fn-binding ability of methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (TrmFO) and its role in M. bovis cytoadherence. The trmFO (MBOV_RS00785) gene was cloned and expressed in E...
August 9, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28791415/impacts-of-the-serum-containing-total-flavonoids-of-ajuga-on-rat-glomerular-mesangial-cells
#13
Lihong Nan, Mei Huang, Wenfang Lai, Ru Jia, Yanfang Zheng, Lan Yang, Qingqing Xie, Weihua Peng
The aim of the current study was to investigate the impacts and possible mechanisms of total flavonoids of Ajuga (TFA) on glomerular mesangial cells (GMC) through in vitro observations of the impacts of TFA‑containing serum on GMC proliferation and extracellular matrix (ECM) secretion in lipopolysaccharides (LPS)‑induced rats. Rat GMC was cultured in vitro, using LPS to stimulate the proliferation of GMC and the secretion of ECM; meanwhile, TFA‑containing serum (TFA‑S) was used for the intervention...
August 8, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28790175/antibody-mediated-blockade-of-jmjd6-interaction-with-collagen-i-exerts-antifibrotic-and-antimetastatic-activities
#14
Silvia Miotti, Alessandro Gulino, Renata Ferri, Mariella Parenza, Agnieszka Chronowska, Daniele Lecis, Sabina Sangaletti, Elda Tagliabue, Claudio Tripodo, Mario P Colombo
JMJD6 is known to localize in the nucleus exerting histone arginine demethylase and lysyl hydroxylase activities. A novel localization of JMJD6 in the extracellular matrix, resulting from its secretion as a soluble protein, was unveiled by a new anti-JMJD6 mAb called P4E11 which was developed to identify new targets in the stroma. Recombinant JMJD6 binds with collagen type I (Coll-I), and distinct JMJD6 peptides interfere with collagen fibrillogenesis, collagen-fibronectin interaction, and adhesion of human tumor cells to the collagen substrate...
August 8, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28789988/the-use-of-retronectin-in-studies-requiring-in-vitro-hiv-1-infection-of-human-hematopoietic-stem-progenitor-cells
#15
Tetsuo Tsukamoto, Seiji Okada
Human immunodeficiency virus (HIV) causes damage, directly or indirectly, to the whole hematopoietic system, including CD34(+) hematopoietic stem/progenitor cells (HSPCs). CXCR4-tropic strains of HIV-1 may affect the function of CD34(+)CXCR4(+) progenitor cells either by infecting the cells or modifying the dynamics of more differentiated hematopoietic cells. However, CD34(+) cells are known for their resistance to HIV-1 infection in vitro, which restricts any detailed analysis of the impact of HIV on HSPCs...
August 5, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28789942/statins-reduce-tgf-beta2-modulation-of-the-extracellular-matrix-in-cultured-astrocytes-of-the-human-optic-nerve-head
#16
Mi-Lyang Kim, Kyung Rim Sung, Jin A Shin, Joo Young Yoon, Joonhee Jang
Statins are cholesterol lowering drugs and have shown beneficial effects on glaucoma. With regard to the mechanism of statin action on glaucoma, we investigated the effects of statins on transforming growth factor-beta 2 (TGF-β2)-induced expression of extracellular matrix (ECM) proteins in human astrocytes of the optic nerve head (ONH) lamina cribrosa (LC). By using primary human ONH astrocytes, we found that both simvastatin and lovastatin inhibited TGF-β2-mediated expression of ECM proteins such as connective tissue growth factor, collagen I, fibronectin, and plasminogen activator inhibitor-1...
August 5, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/28782391/in-vitro-expansion-of-cd-133-cells-derived-from-umbilical-cord-blood-in-poly-l-lactic-acid-plla-scaffold-coated-with-fibronectin-and-collagen
#17
Maryam Islami, Yousef Mortazavi, Masoud Soleimani, Samad Nadri
CONTEXT: Due to their renewal and potency, umbilical cord blood (UCB) stem cells have the ability to proliferate and serve as an attractive alternative source for bone marrow transplantation. However, insufficient number of haematopoietic stem cells (HSCs) in UCB is still a major constraint in clinical applications. OBJECTIVE: In vitro expansion of stem cells on fibronectin (Fn)-coated poly-L-lactic acid (PLLA) scaffold can be a proper way to overcome this limitation...
August 6, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28780788/-therapeutic-effect-of-mir-489-in-a-mouse-model-of-silica-induced-matured-pulmonary-fibrosis
#18
S X Jin, Q Y Wu, W W Yan, C H Ni
Objective: To explore the potential therapeutic role of miR-489 in silica-induced pulmonary fibrosis mouse models. Methods: A total of 32 C57BL/6 male mice were randomly divided into four groups: saline, silica, silica plus miRNA control and silica plus miR-489 agomir (n=8 in each group) . The mice were instilled with silica particles suspended in saline or sterile saline intratracheally. Subsequently, miR-489 agomir or miRNA control was injected via the tail vein into each mouse at days 28, 35, 42 and 49, the miR-489 levels, histological examination, collagen deposition, fibrotic biomarkers (E-cadherin, α-SMA, Vimentin, Fibronectin) and transforming growth factor-β(1) (TGF-β(1)) protein levels in mouse lung tissues were measured...
May 20, 2017: Chinese Journal of Industrial Hygiene and Occupational Diseases
https://www.readbyqxmd.com/read/28780243/clinical-and-molecular-characteristics-of-infective-%C3%AE-hemolytic-streptococcal-endocarditis
#19
Oddvar Oppegaard, Haima Mylvaganam, Steinar Skrede, Stina Jordal, Marte Glambek, Bård Reiakvam Kittang
Streptococcus pyogenes (S. pyogenes) and Streptococcus dysgalactiae subspecies equisimilis (SDSE) cause considerable morbidity and mortality, and show similarities in disease manifestations and pathogenic mechanisms. Their involvement in infective endocarditis, however, has not been well described. Invasive S. pyogenes and SDSE infections in Health Region Bergen, Norway, in the period 1999-2013 were reviewed, and sixteen cases of endocarditis were identified. The median duration of symptoms was 2.5days, the frequency of embolic events 50%, 38% received valve replacement and the 30-day mortality was 25%...
June 30, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28778116/caveolin-1-restricts-group-a-streptococcus-invasion-of-non-phagocytic-host-cells
#20
Jin Yan Lim, Timothy C Barnett, Michele Bastiani, Kerrie-Ann McMahon, Charles Ferguson, Richard I Webb, Robert G Parton, Mark J Walker
Caveolae are composed of two major proteins, caveolin-1 (CAV1) and cavin-1/polymerase transcript factor I (CAVIN1). Here we demonstrate that CAV1 levels modulate invasion of group A Streptococcus (GAS) into non-phagocytic mammalian cells. GAS showed enhanced internalization into CAV1 knockout mouse embryonic fibroblasts (MEFs), and CAV1 knockdown human epithelial HEp-2 cells, whereas over-expression of CAV1 in HEp-2 cells reduced GAS invasion. This effect was not dependent on the expression of the GAS fibronectin binding protein SfbI, which had previously been implicated in caveolae-mediated uptake...
August 4, 2017: Cellular Microbiology
keyword
keyword
120977
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"