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fp essential

Karin Nienhaus, Gerd Ulrich Nienhaus
Photoactivatable fluorescent proteins (FPs) have become essential markers for nanoscopy on live specimens. In this issue of ACS Nano, Wang et al. present a reversibly photoswitching FP, GMars-Q, which they promote as an advanced marker for RESOLFT imaging because of its low residual intensity in the off state and low switching fatigue. Here, we explain the observed peculiar photobleaching behavior of GMars-Q by a mechanism that involves efficient shelving of proteins in dark states, resulting in low switching fatigue and low residual off intensity...
October 10, 2016: ACS Nano
Zhichao Dong, Haobo Cheng
A hybrid algorithm is proposed in this study for demodulating a single spatial carrier fringe pattern (FP) of interferometric measurement, which essentially combines the spatial carrier phase shift (SCPS) method and Fourier transform (FT) method. It firstly extracts three phase-shifted FPs from a single spatial carrier FP, then employs the FT method and a subtraction operation to determine the phase shift of three phase-shifted FPs, and finally retrieves the phase map using a least-square phase shift algorithm...
September 20, 2016: Applied Optics
P Guerreschi, P-E Gabert, D Labbé, V Martinot-Duquennoy
Facial palsy (FP) in children is congenital or acquired. When present at birth (congenital), etiologies are mostly traumatic and rarely developmental. Acquired FP needs investigation. Research on the etiology helps to determine prognostic and treatment. At most times, no specific cause is found. Treatment of idiopathic FP consists of early oral corticosteroid therapy and ocular protection. Treating the sequelae is essential and the physician has to consider the dynamic balance of both sides of the face. Dynamic rehabilitation should mainly concern the inferior facial third...
October 2016: Annales de Chirurgie Plastique et Esthétique
Elisabeth Pfeiffenberger, Reinhard Sigl, Stephan Geley
RNA interference (RNAi) has become an essential technology for functional gene analysis. Its success depends on the effective expression of target gene-specific RNAi-inducing small double-stranded interfering RNA molecules (siRNAs). Here, were describe the use of a recently developed lentiviral RNAi system that allows the rapid generation of stable cell lines with inducible RNAi based on conditional expression of double-stranded short hairpin RNA (shRNA). These lentiviral vectors can be generated rapidly using the GATEWAY recombination cloning technology...
2016: Methods in Molecular Biology
Gabor Perlaki, Sarolta Szekeres, Gergely Orsi, Laszlo Papp, Balazs Suha, Szilvia Anett Nagy, Tamas Doczi, Jozsef Janszky, Katalin Zambo, Norbert Kovacs
INTRODUCTION: Dopamine transporter imaging with (123)I-FP-CIT single photon emission computed tomography (SPECT) is helpful for the differential diagnosis between Parkinsonian syndrome (PS) and essential tremor (ET). Although visual assessment and time-consuming manual evaluation techniques are readily available, a fully objective and automated dopamine transporter quantification technique is always preferable, at least in research and follow-up investigations. Our aim was to develop a novel automated magnetic resonance imaging (MRI)-based evaluation technique of dopamine transporter SPECT images and to compare its diagnostic accuracy with those of the gold-standard visual grading and manual dopamine transporter binding quantification methods...
August 2016: Parkinsonism & related Disorders
M Wang, H Zhang, Q-M Liu, Y Sun, Z Li, W-H Liu, X-H He, J Song, Y-X Wang
The successful foamy viruses (FVs) infection includes at least two essential events, attachment to the cell surface and fusion of the viral envelope with the cell membrane. For the FVs, membrane fusion between virus and cell is mediated by envelope glycoprotein (Env) transmembrane (TM) subunit gp47. Compared with other retroviruses, FV TM subunit shares a similar but not identical structural characteristic. This paper focuses on in sillico analyses of all 15 available FV TM subunits gp47 based on their amino acid sequences...
June 2016: Acta Virologica
Nancy L Hancock, Carla J Chibwesha, Samuel Bosomprah, Jonathan Newman, Mwangelwa Mubiana-Mbewe, Elizabeth Siyama Sitali, Carolyn Bolton-Moore, Clara Mbwili-Muleya, Benjamin H Chi
BACKGROUND: Family planning (FP) is an essential health service and an important part of comprehensive HIV care. However, there is limited information about the contraceptive needs of people living with HIV in sub-Saharan Africa, which in turn has hampered efforts to expand and integrate FP services into existing HIV programs. METHODS: We performed a cross-sectional survey to determine FP prevalence and predictors among HIV-positive women and men attending 18 public antiretroviral therapy (ART) clinics in Lusaka, Zambia...
2016: BMC Public Health
Kazuya Horiuchi, Keita Kasahara, Yusuke Kuroda, Haruna Morohoshi, Yosuke Hagiwara, Gen Ishii
PURPOSE: A combination therapy with inhaled corticosteroid (ICS) and a long-acting β agonist (LABA) is the standard treatment for asthmatic patients, and step-down treatment is recommended once control has been achieved. However, little data exist that evaluate the long-term outcomes after step-down treatment. OBJECTIVE: To compare the long-term outcomes of step-down therapy with ICS/LABA or ICS alone for asthmatic patients who have achieved well-controlled asthma by the ICS (250 µg fluticasone)/LABA (50 µg salmeterol) combination (SFC, two puffs per day)...
2016: Journal of Asthma and Allergy
N S Korse, M P J Nicolai, S Both, C L A Vleggeert-Lankamp, H W Elzevier
PURPOSE: Due to advancing insights, discussing fertility in spinal care is an emerging topic. Studies among neurosurgeons to evaluate clinical practice about discussing fertility are non-existent. The aim of this study is to review knowledge, attitude and practice patterns regarding discussing fertility in spinal care. METHODS: Dutch neurosurgeons and residents were sent a mail-based questionnaire addressing attitude, knowledge and practice patterns regarding discussing fertility...
September 2016: European Spine Journal
Xiuyuan Ou, Wangliang Zheng, Yiwei Shan, Zhixia Mu, Samuel R Dominguez, Kathryn V Holmes, Zhaohui Qian
UNLABELLED: The fusion peptides (FP) play an essential role in fusion of viral envelope with cellular membranes. The location and properties of the FPs in the spike (S) glycoproteins of different coronaviruses (CoV) have not yet been determined. Through amino acid sequence analysis of S proteins of representative CoVs, we identified a common region as a possible FP (pFP) that shares the characteristics of FPs of class I viral fusion proteins, including high Ala/Gly content, intermediate hydrophobicity, and few charged residues...
June 15, 2016: Journal of Virology
Jocelyn Barbosa, Kyubum Lee, Sunwon Lee, Bilal Lodhi, Jae-Gu Cho, Woo-Keun Seo, Jaewoo Kang
BACKGROUND: Facial palsy or paralysis (FP) is a symptom that loses voluntary muscles movement in one side of the human face, which could be very devastating in the part of the patients. Traditional methods are solely dependent to clinician's judgment and therefore time consuming and subjective in nature. Hence, a quantitative assessment system becomes apparently invaluable for physicians to begin the rehabilitation process; and to produce a reliable and robust method is challenging and still underway...
2016: BMC Medical Imaging
Ahmed Bouhouche, Wafaa Regragui, Hind Lamghari, Khadija Khaldi, Nazha Birouk, Safaa Lytim, Soufiane Bellamine, Yamna Kriouile, Naima Bouslam, El Hachmia Ait Ben Haddou, Mustapha Alaoui Faris, Ali Benomar, Mohamed Yahyaoui
BACKGROUND: Huntington's disease (HD) occurs worldwide with prevalence varying from 0.1 to 10/100,000 depending of the ethnic origin. Since no data is available in the Maghreb population, the aim of this study is to describe clinical and genetic characteristics of Huntington patients of Moroccan origin. METHODS: Clinical and genetics data of 21 consecutive patients recruited from 2009 to 2014 from the outpatient clinic of six medical centers were analyzed. Statistical analysis was performed using descriptive statistics...
December 2015: African Health Sciences
Doranda Perdomo, Mélanie Bonhivers, Derrick R Robinson
Sub-species of Trypanosoma brucei are the causal agents of human African sleeping sickness and Nagana in domesticated livestock. These pathogens have developed an organelle-like compartment called the flagellar pocket (FP). The FP carries out endo- and exocytosis and is the only structure this parasite has evolved to do so. The FP is essential for parasite viability, making it an interesting structure to evaluate as a drug target, especially since it has an indispensible cytoskeleton component called the flagellar pocket collar (FPC)...
2016: Cells
David S Pescador, Ángela Sierra-Almeida, Pablo J Torres, Adrián Escudero
Assessing freezing community response and whether freezing resistance is related to other functional traits is essential for understanding alpine community assemblages, particularly in Mediterranean environments where plants are exposed to freezing temperatures and summer droughts. Thus, we characterized the leaf freezing resistance of 42 plant species in 38 plots at Sierra de Guadarrama (Spain) by measuring their ice nucleation temperature, freezing point (FP), and low-temperature damage (LT50), as well as determining their freezing resistance mechanisms (i...
2016: Frontiers in Plant Science
Dorit Barlevy, Tenzin Wangmo, Bernice S Elger, Vardit Ravitsky
PURPOSE: Due to improved survival rates of adolescent cancers, increasing attention is paid to addressing patients' future quality of life after treatment. A salient quality of life issue for adolescent survivors is reproductive capacity. Discussing oncofertility, that is, cancer treatment's possible effects on fertility and fertility preservation (FP) options, is a means to address this concern by informing patients and parents regarding options available to them to prevent future struggles with infertility...
June 2016: Journal of Adolescent and Young Adult Oncology
Juliana Schwaab, Mohamad Jawhar, Nicole Naumann, Annette Schmitt-Graeff, Alice Fabarius, Hans-Peter Horny, Nicholas C P Cross, Wolf-Karsten Hofmann, Andreas Reiter, Georgia Metzgeroth
The FIP1L1-PDGFRA (FP) fusion gene is identified in a substantial proportion of patients with eosinophilia-associated myeloproliferative neoplasms (MPN-eo) who subsequently achieve rapid and durable remissions on imatinib. In the initial diagnostic work-up of hypereosinophilia (HE), histologic and immunohistochemical evaluation of a bone marrow (BM) core biopsy is considered essential for the differentiation between reactive hypereosinophilia (HER), MPN-eo and hypereosinophilic syndrome (HES). We therefore retrospectively analysed the initial reports of BM core biopsies from 116 patients who were subsequently identified as FP positive (FP+, n = 56) or FP negative/corticosteroid-responsive HER or HES (n = 60)...
March 2016: Annals of Hematology
Amira Gaber, Mona F Taher, Manal Abdel Wahed
Assessment of facial paralysis (FP) and quantitative grading of facial asymmetry are essential in order to quantify the extent of the condition as well as to follow its improvement or progression. As such, there is a need for an accurate quantitative grading system that is easy to use, inexpensive and has minimal inter-observer variability. A comprehensive automated system to quantify and grade FP is the main objective of this work. An initial prototype has been presented by the authors. The present research aims to enhance the accuracy and robustness of one of this system's modules: the resting symmetry module...
2015: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Isha A Ten Have, Michel Pj van den Bekerom, Derek Fp van Deurzen, Michel Gjs Hageman
Medical treatment of patients inherently entails the risk of undesired complication or side effects. It is essential to inform the patient about the expected outcomes, but also the possible undesired outcomes. The patients preference and values regarding the potential outcomes should be involved in the decision making process. Even though many orthopaedic surgeons are positive towards shared decision-making, it is minimally introduced in the orthopaedic daily practice and decision-making is still mostly physician based...
December 18, 2015: World Journal of Orthopedics
Yo Sonoda, Kimihiko Mizutani, Bunzo Mikami
Spo0M is a sporulation-control protein that is thought to play an essential role in the early stage of endospore formation. While little is known about the functions of Spo0M, a recent phylogenetic study suggests that, based on its amino-acid sequence, Spo0M might belong to the arrestin clan. The crystal structure of the Spo0M protein was determined at a resolution of 2.3 Å. Ten amino acids at the end of the N-terminus were removed to improve the thermal stability of the purified Spo0M protein and the crystal structure of Spo0M was determined by SAD...
December 2015: Acta Crystallographica. Section F, Structural Biology Communications
Prince P Mutalemwa, William N Kisinza, Michael Munga, Janesta A E Urassa, Stafford Kibona, Upendo Mwingira, Christina Lasway, Stella Kilima, Filemoni Tenu, Stella Mujaya, William J Kisoka
In Tanzania, reproductive health and HIV services are coordinated by the Ministry of Health and Social Welfare in two separate units namely Reproductive and Child Health Section and the National AIDS Control Programme. The importance of integrating the two services that are vertically run is expected to improve access to and uptake of key essential services and extend coverage to underserved and vulnerable populations and thus minimizing missed opportunities. Experts around the world recognize the central role of Sexual and Reproductive Health (SRH) services in preventing HIV infection...
April 2013: Tanzania Journal of Health Research
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