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https://www.readbyqxmd.com/read/28419571/characterization-of-mesenchymal-stem-cells-in-bovine-endometrium-during-follicular-phase-of-oestrous-cycle
#1
E Lara, N Rivera, D Rojas, L L Rodríguez-Alvarez, F O Castro
Stem cells have been postulated as responsible for cell regeneration in highly and continuously regenerative tissues such as the endometrium. Few studies in cattle have identified and specified the presence of stem cells in the endometrium during the oestrous cycle. The aim of this study was to investigate the presence of mesenchymal stem cells (MSCs) in the bovine endometrium during the follicular phase (FP) of the oestrous cycle. Uterine tissue was collected in the time-frame comprising day 18 of the cycle and ovulation (day 0)...
April 16, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28404198/molecular-fluorescence-guided-surgery-of-peritoneal-carcinomatosis-of-colorectal-origin-a-single-centre-feasibility-study
#2
Niels J Harlaar, Marjory Koller, Steven J de Jongh, Barbara L van Leeuwen, Patrick H Hemmer, Schelto Kruijff, Robert J van Ginkel, Lukas B Been, Johannes S de Jong, Gursah Kats-Ugurlu, Matthijs D Linssen, Annelies Jorritsma-Smit, Marleen van Oosten, Wouter B Nagengast, Vasilis Ntziachristos, Gooitzen M van Dam
BACKGROUND: Optimum cytoreductive surgery combined with hyperthermic intraperitoneal chemotherapy (HIPEC) is essential for the curative treatment of peritoneal carcinomatosis of colorectal origin. At present, surgeons depend on visual inspection and palpation for tumour detection. Improved detection of tumour tissue using molecular fluorescence-guided surgery could not only help attain a complete cytoreduction of metastatic lesions, but might also prevent overtreatment by avoiding resection of benign lesions...
December 2016: Lancet. Gastroenterology & Hepatology
https://www.readbyqxmd.com/read/28336060/recent-advances-in-development-of-genetically-encoded-fluorescent-sensors
#3
Lynn Sanford, Amy Palmer
Genetically encoded fluorescent sensors are essential tools in modern biological research, and recent advances in fluorescent proteins (FPs) have expanded the scope of sensor design and implementation. In this review we compare different sensor platforms, including Förster resonance energy transfer (FRET) sensors, fluorescence-modulated single FP-based sensors, translocation sensors, complementation sensors, and dimerization-based sensors. We discuss elements of sensor design and engineering for each platform, including the incorporation of new types of FPs and sensor screening techniques...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28326779/illuminating-photochemistry-of-an-excitation-ratiometric-fluorescent-protein-calcium-biosensor
#4
Longteng Tang, Yanli Wang, Weimin Liu, Yongxin Zhao, Robert E Campbell, Chong Fang
Fluorescent protein (FP)-based biosensors have become an important and promising tool to track metal ion movement inside living systems. Their working principles after light irradiation, however, remain elusive. To facilitate the rational design of biosensors, we dissect the fluorescence modulation mechanism of a newly developed excitation ratiometric green FP-based Ca(2+) biosensor, GEX-GECO1, using femtosecond stimulated Raman spectroscopy (FSRS) in the electronic excited state. Upon 400 nm photoexcitation, characteristic vibrational marker bands at ∼1180 and 1300 cm(-1) show concomitant decay and rise dynamics, probing the progression of an ultrafast excited state proton transfer (ESPT) reaction...
March 30, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28299031/continuity-of-care-evaluation-the-view-of-patients-and-professionals-about-urban-family-physician-program
#5
Vahid Kohpeima Jahromi, Mohammad Hossein Mehrolhassani, Reza Dehnavieh, Hosein Saberi Anari
BACKGROUND: A responsibility of the family physician (FP) and one of the four aspects of the delivery of primary care services is continuity of care (COC). This study aimed to determine the COC of health care in urban health centers. METHODS: Between September 2015 and March 2016, we conducted a cross-sectional study using Primary Care Evaluation Tool questionnaires with multistage stratified cluster sample of FPs (n = 141) and patients (n = 710) in two provinces in Iran, Fars and Mazandaran...
2017: International Journal of Preventive Medicine
https://www.readbyqxmd.com/read/28264884/functional-and-structural-insight-into-properdin-control-of-complement-alternative-pathway-amplification
#6
Dennis V Pedersen, Lubka Roumenina, Rasmus K Jensen, Trine Af Gadeberg, Chiara Marinozzi, Capucine Picard, Tania Rybkine, Steffen Thiel, Uffe Bs Sørensen, Cordula Stover, Veronique Fremeaux-Bacchi, Gregers R Andersen
Properdin (FP) is an essential positive regulator of the complement alternative pathway (AP) providing stabilization of the C3 and C5 convertases, but its oligomeric nature challenges structural analysis. We describe here a novel FP deficiency (E244K) caused by a single point mutation which results in a very low level of AP activity. Recombinant FP E244K is monomeric, fails to support bacteriolysis, and binds weakly to C3 products. We compare this to a monomeric unit excised from oligomeric FP, which is also dysfunctional in bacteriolysis but binds the AP proconvertase, C3 convertase, C3 products and partially stabilizes the convertase...
April 13, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28138820/development-of-extended-release-oral-flexible-tablet-er-oft-formulation-for-pediatric-and-geriatric-compliance-an-age-appropriate-formulation
#7
Prabagaran Chandrasekaran, Ruckmani Kandasamy
Development of age-appropriate formulation suitable for pediatric and geriatric patients involves various challenges. The objective of this research was to develop extended-release oral flexible tablet (ER-OFT) formulation using carbamazepine (CBZ) as model drug for pediatric and geriatric compliance. ER-microparticles of carbamazepine, a BCS class 2, and narrow therapeutic index (NTI) drug, were prepared using ethyl cellulose as matrixing polymer and hypromellose as hydrophilic pore former. Microparticles were prepared using high-shear granulator fitted with an atomizing spray system...
January 30, 2017: AAPS PharmSciTech
https://www.readbyqxmd.com/read/28092031/expression-and-production-of-sh2-domain-proteins
#8
Bernard A Liu, Mari Ogiue-Ikeda, Kazuya Machida
The Src Homology 2 (SH2) domain lies at the heart of phosphotyrosine signaling, coordinating signaling events downstream of receptor tyrosine kinases (RTKs), adaptors, and scaffolds. Over a hundred SH2 domains are present in mammals, each having a unique specificity which determines its interactions with multiple binding partners. One of the essential tools necessary for studying and determining the role of SH2 domains in phosphotyrosine signaling is a set of soluble recombinant SH2 proteins. Here we describe methods, based on a broad experience with purification of all SH2 domains, for the production of SH2 domain proteins needed for proteomic and biochemical-based studies such as peptide arrays, mass-spectrometry, protein microarrays, reverse-phase microarrays, and high-throughput fluorescence polarization (HTP-FP)...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28003005/targeting-cysteine-proteases-from-plasmodium-falciparum-a-general-overview-rational-drug-design-and-computational-approaches-for-drug-discovery
#9
Boris D Bekono, Fidele Ntie-Kang, Luc C Owono Owono, Eugene Megnassan
The Plasmodium falciparum cysteine proteases, also known as falcipains, are involved in different erythrocytic cycle processes of the malaria parasite, e.g. hydrolysis of host haemoglobin, erythrocyte invasion, and erythrocyte rupture. With the biochemical characterization of four falcipains so far, FP-2 (falcipain-2) and FP-3 (falcipain-3), members of the papain-like CAC1 family, are essential haemoglobinases. They could therefore be referred to as potential anti-malarial drug targets in the search for novel therapies, which could ease the burden caused by the increasing resistance to current anti-malarial drugs...
December 21, 2016: Current Drug Targets
https://www.readbyqxmd.com/read/27992813/which-dsm-validated-tools-for-diagnosing-depression-are-usable-in-primary-care-research-a-systematic-literature-review
#10
P Nabbe, J Y Le Reste, M Guillou-Landreat, M A Munoz Perez, S Argyriadou, A Claveria, M I Fernández San Martín, S Czachowski, H Lingner, C Lygidakis, A Sowinska, B Chiron, J Derriennic, A Le Prielec, B Le Floch, T Montier, H Van Marwijk, P Van Royen
INTRODUCTION: Depression occurs frequently in primary care. Its broad clinical variability makes it difficult to diagnose. This makes it essential that family practitioner (FP) researchers have validated tools to minimize bias in studies of everyday practice. Which tools validated against psychiatric examination, according to the major depression criteria of DSM-IV or 5, can be used for research purposes? METHOD: An international FP team conducted a systematic review using the following databases: Pubmed, Cochrane and Embase, from 2000/01/01 to 2015/10/01...
December 16, 2016: European Psychiatry: the Journal of the Association of European Psychiatrists
https://www.readbyqxmd.com/read/27892522/sars-cov-fusion-peptides-induce-membrane-surface-ordering-and-curvature
#11
Luis G M Basso, Eduardo F Vicente, Edson Crusca, Eduardo M Cilli, Antonio J Costa-Filho
Viral membrane fusion is an orchestrated process triggered by membrane-anchored viral fusion glycoproteins. The S2 subunit of the spike glycoprotein from severe acute respiratory syndrome (SARS) coronavirus (CoV) contains internal domains called fusion peptides (FP) that play essential roles in virus entry. Although membrane fusion has been broadly studied, there are still major gaps in the molecular details of lipid rearrangements in the bilayer during fusion peptide-membrane interactions. Here we employed differential scanning calorimetry (DSC) and electron spin resonance (ESR) to gather information on the membrane fusion mechanism promoted by two putative SARS FPs...
November 28, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27873254/fluorescence-based-real-time-activity-assays-to-identify-rnase-p-inhibitors
#12
Yu Chen, Xin Liu, Nancy Wu, Carol A Fierke
Transfer RNA is transcribed as precursor molecules that are processed before participating in translation catalyzed by the ribosome. Ribonuclease P is the endonuclease that catalyzes the 5' end maturation of precursor tRNA and it is essential for cell survival. Bacterial RNase P has a distinct subunit composition compared to the eukaryal counterparts; therefore, it is an attractive antibacterial target. Here, we describe a real-time fluorescence-based RNase P activity assay using fluorescence polarization/anisotropy with a 5' end fluorescein-labeled pre-tRNA(Asp) substrate...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27872622/an-evaluation-of-20-years-of-eu-framework-programme-funded-immune-mediated-inflammatory-translational-research-in-non-human-primates
#13
Krista G Haanstra, Margreet Jonker, Bert A 't Hart
Aging western societies are facing an increasing prevalence of chronic inflammatory and degenerative diseases for which often no effective treatments exist, resulting in increasing health-care expenditure. Despite high investments in drug development, the number of promising new drug candidates decreases. We propose that preclinical research in non-human primates can help to bridge the gap between drug discovery and drug prescription. Translational research covers various stages of drug development of which preclinical efficacy tests in valid animal models is usually the last stage...
2016: Frontiers in Immunology
https://www.readbyqxmd.com/read/27863543/reduced-humoral-immunity-and-atypical-cell-mediated-immunity-in-response-to-vaccination-in-cows-naturally-infected-with-bovine-leukemia-virus
#14
Meredith C Frie, Kelly R Sporer, Joseph C Wallace, Roger K Maes, Lorraine M Sordillo, Paul C Bartlett, Paul M Coussens
Bovine leukemia virus (BLV) is a retrovirus that is widely distributed across US dairy herds: over 83% of herds are BLV-infected and within-herd infection rates can approach 50%. BLV infection reduces both animal longevity and milk production and can interfere with normal immune health. With such a high prevalence of BLV infection in dairy herds, it is essential to understand the circumstances by which BLV negatively affects the immune system of infected cattle. To address this question, BLV- and BLV+ adult, lactating Holstein dairy cows were vaccinated with Bovi-Shield GOLD(®) FP(®) 5 L5 HB and their immune response to vaccination was measured over the course of 28days...
December 2016: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/27795325/probing-for-binding-regions-of-the-ftsz-protein-surface-through-site-directed-insertions-discovery-of-fully-functional-ftsz-fluorescent-proteins
#15
Desmond A Moore, Zakiya N Whatley, Chandra P Joshi, Masaki Osawa, Harold P Erickson
FtsZ, a bacterial tubulin homologue, is a cytoskeletal protein that assembles into protofilaments that are one subunit thick. These protofilaments assemble further to form a "Z ring" at the center of prokaryotic cells. The Z ring generates a constriction force on the inner membrane and also serves as a scaffold to recruit cell wall remodeling proteins for complete cell division in vivo One model of the Z ring proposes that protofilaments associate via lateral bonds to form ribbons; however, lateral bonds are still only hypothetical...
January 1, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/27774138/discovery-of-fluorescence-polarization-probe-for-the-elisa-based-antagonist-screening-of-%C3%AE-1-adrenergic-receptors
#16
Zhao Ma, Zhenzhen Liu, Tianyu Jiang, Tianchao Zhang, Huateng Zhang, Lupei Du, Minyong Li
High-throughput screening (HTS) of ligand library to find new active molecules for G protein-coupled receptors is still a major interest, as well as an actual challenge. Fluorescence polarization (FP) assay portrays an essential role in HTS; however, in many cases, it was restricted by the absence of FP probes, the narrow measurement window, and low signal-to-noise (S/N) ratio. Herein, based on the modification of our previous probe 1 (QFL), we discovered an FP probe 3 (QGGFL) for α1-adrenergic receptors (α1-ARs), which has satisfactory fluorescence intensity, specific binding ability to receptors, and suitable fluorescence properties that were compatible with the filters in the FP system...
October 13, 2016: ACS Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/27723301/photoswitchable-fluorescent-proteins-do-not-always-look-on-the-bright-side
#17
Karin Nienhaus, Gerd Ulrich Nienhaus
Photoactivatable fluorescent proteins (FPs) have become essential markers for nanoscopy on live specimens. In this issue of ACS Nano, Wang et al. present a reversibly photoswitching FP, GMars-Q, which they promote as an advanced marker for RESOLFT imaging because of its low residual intensity in the off state and low switching fatigue. Here, we explain the observed peculiar photobleaching behavior of GMars-Q by a mechanism that involves efficient shelving of proteins in dark states, resulting in low switching fatigue and low residual off intensity...
October 10, 2016: ACS Nano
https://www.readbyqxmd.com/read/27661584/hybrid-algorithm-for-phase-retrieval-from-a-single-spatial-carrier-fringe-pattern
#18
Zhichao Dong, Haobo Cheng
A hybrid algorithm is proposed in this study for demodulating a single spatial carrier fringe pattern (FP) of interferometric measurement, which essentially combines the spatial carrier phase shift (SCPS) method and Fourier transform (FT) method. It firstly extracts three phase-shifted FPs from a single spatial carrier FP, then employs the FT method and a subtraction operation to determine the phase shift of three phase-shifted FPs, and finally retrieves the phase map using a least-square phase shift algorithm...
September 20, 2016: Applied Optics
https://www.readbyqxmd.com/read/27637411/-facial-palsy-in-children
#19
P Guerreschi, P-E Gabert, D Labbé, V Martinot-Duquennoy
Facial palsy (FP) in children is congenital or acquired. When present at birth (congenital), etiologies are mostly traumatic and rarely developmental. Acquired FP needs investigation. Research on the etiology helps to determine prognostic and treatment. At most times, no specific cause is found. Treatment of idiopathic FP consists of early oral corticosteroid therapy and ocular protection. Treating the sequelae is essential and the physician has to consider the dynamic balance of both sides of the face. Dynamic rehabilitation should mainly concern the inferior facial third...
October 2016: Annales de Chirurgie Plastique et Esthétique
https://www.readbyqxmd.com/read/27317178/conditional-rnai-using-the-lentiviral-gltr-system
#20
Elisabeth Pfeiffenberger, Reinhard Sigl, Stephan Geley
RNA interference (RNAi) has become an essential technology for functional gene analysis. Its success depends on the effective expression of target gene-specific RNAi-inducing small double-stranded interfering RNA molecules (siRNAs). Here, were describe the use of a recently developed lentiviral RNAi system that allows the rapid generation of stable cell lines with inducible RNAi based on conditional expression of double-stranded short hairpin RNA (shRNA). These lentiviral vectors can be generated rapidly using the GATEWAY recombination cloning technology...
2016: Methods in Molecular Biology
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