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Intra-Amniotic Infection

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https://www.readbyqxmd.com/read/27884950/neutrophil-extracellular-traps-in-the-amniotic-cavity-of-women-with-intra-amniotic-infection-a-new-mechanism-of-host-defense
#1
Nardhy Gomez-Lopez, Roberto Romero, Yi Xu, Derek Miller, Ronald Unkel, Majid Shaman, Suzanne M Jacques, Bogdan Panaitescu, Valeria Garcia-Flores, Sonia S Hassan
OBJECTIVE: Neutrophil extracellular traps (NETs) control microbial infections through their antimicrobial activities attributed to DNA, histones, granules, and cytoplasmic proteins (eg, elastase). Intra-amniotic infection is characterized by the influx of neutrophils into the amniotic cavity; therefore, the aim of this study was to determine whether amniotic fluid neutrophils form NETs in this inflammatory process. METHODS: Amniotic fluid samples from women with intra-amniotic infection (n = 15) were stained for bacteria detection using fluorescent dyes...
November 23, 2016: Reproductive Sciences
https://www.readbyqxmd.com/read/27794570/limiting-the-exposure-of-select-fetuses-to-intrauterine-infection-inflammation-improves-short-term-neonatal-outcomes-in-preterm-premature-rupture-of-membranes
#2
Karen L Archabald, Irina A Buhimschi, Mert O Bahtiyar, Antonette T Dulay, Sonya S Abdel-Razeq, Christian M Pettker, Heather S Lipkind, John T Hardy, Megan E McCarthy, Guomao Zhao, Vineet Bhandari, Catalin S Buhimschi
BACKGROUND: To improve neonatal outcomes in pregnancies at heightened risk for early-onset neonatal sepsis (EONS), there is a need to identify fetuses that benefit from expectant management as opposed to early delivery. Detectable haptoglobin and haptoglobin-related protein (Hp&HpRP switch-on status) in cord blood has been proposed as a biomarker of antenatal exposure to intra-amniotic infection and/or inflammation (IAI), an important determinant of EONS. SUBJECTS AND METHODS: We analyzed 185 singleton newborns delivered secondary to preterm premature rupture of membranes (PPROM)...
October 29, 2016: Fetal Diagnosis and Therapy
https://www.readbyqxmd.com/read/27763883/clinical-chorioamnionitis-at-term-vii-the-amniotic-fluid-cellular-immune-response
#3
Alicia Martinez-Varea, Roberto Romero, Yi Xu, Derek Miller, Ahmed I Ahmed, Piya Chaemsaithong, Noppadol Chaiyasit, Lami Yeo, Majid Shaman, Kia Lannaman, Benjamin Cher, Sonia S Hassan, Nardhy Gomez-Lopez
OBJECTIVES: 1) To characterize the cellular composition of the amniotic fluid of patients diagnosed with clinical chorioamnionitis at term, as a function of the presence or absence of microorganisms determined by cultivation techniques, and 2) to characterize the cytokine production by white blood cells present in the amniotic fluid using flow cytometry-based techniques. MATERIALS AND METHODS: Amniotic fluid samples from 20 women who had the diagnosis of clinical chorioamnionitis at term were analyzed using cultivation techniques (for aerobic and anaerobic bacteria as well as genital Mycoplasmas)...
October 20, 2016: Journal of Perinatal Medicine
https://www.readbyqxmd.com/read/27591320/fetal-cd103-il-17-producing-group-3-innate-lymphoid-cells-represent-the-dominant-lymphocyte-subset-in-human-amniotic-fluid
#4
Nicole Marquardt, Martin A Ivarsson, Erik Sundström, Elisabet Åkesson, Elisa Martini, Liv Eidsmo, Jenny Mjösberg, Danielle Friberg, Marius Kublickas, Sverker Ek, Gunilla Tegerstedt, Åke Seiger, Magnus Westgren, Jakob Michaëlsson
Amniotic fluid (AF) surrounds the growing fetus, and cells derived from AF are commonly used for diagnosis of genetic diseases. Intra-amniotic infections are strongly linked to preterm birth, which is the leading cause of perinatal mortality worldwide. Surprisingly little is known, however, about mature hematopoietic cells in AF, which could potentially be involved in immune responses during pregnancy. In this study, we show that the dominating population of viable CD45(+) cells in AF is represented by a subset of fetal CD103(+) group 3 innate lymphoid cells (ILCs) producing high levels of IL-17 and TNF...
September 2, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/27583406/membrane-vesicles-of-group-b-streptococcus-disrupt-feto-maternal-barrier-leading-to-preterm-birth
#5
Manalee Vishnu Surve, Anjali Anil, Kshama Ganesh Kamath, Smita Bhutda, Lakshmi Kavitha Sthanam, Arpan Pradhan, Rohit Srivastava, Bhakti Basu, Suryendu Dutta, Shamik Sen, Deepak Modi, Anirban Banerjee
Infection of the genitourinary tract with Group B Streptococcus (GBS), an opportunistic gram positive pathogen, is associated with premature rupture of amniotic membrane and preterm birth. In this work, we demonstrate that GBS produces membrane vesicles (MVs) in a serotype independent manner. These MVs are loaded with virulence factors including extracellular matrix degrading proteases and pore forming toxins. Mice chorio-decidual membranes challenged with MVs ex vivo resulted in extensive collagen degradation leading to loss of stiffness and mechanical weakening...
September 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27583392/comparison-of-amniotic-fluid-matrix-metalloproteinase-8-and-cathelicidin-in-the-diagnosis-of-intra-amniotic-infection
#6
T Myntti, L Rahkonen, A Pätäri-Sampo, M Tikkanen, T Sorsa, J Juhila, O Helve, S Andersson, J Paavonen, V Stefanovic
OBJECTIVE: To evaluate the association of amniotic fluid (AF) matrix metalloproteinase-8 (MMP-8) and cathelicidin concentrations with microbial invasion of the amniotic cavity (MIAC) in pregnancies with preterm prelabor rupture of the membranes or intact membranes. STUDY DESIGN: Amniocentesis was performed in 54 singleton pregnancies between 22(+0) and 34(+2) gestational weeks with suspected intra-amniotic infection. AF-MMP-8 was analysed by immunoassay and AF-cathelicidin by commercial ELISA...
December 2016: Journal of Perinatology: Official Journal of the California Perinatal Association
https://www.readbyqxmd.com/read/27452435/comprehensive-rna-profiling-of-villous-trophoblast-and-decidua-basalis-in-pregnancies-complicated-by-preterm-birth-following-intra-amniotic-infection
#7
William E Ackerman, Irina A Buhimschi, Haley R Eidem, David C Rinker, Antonis Rokas, Kara Rood, Guomao Zhao, Taryn L Summerfield, Mark B Landon, Catalin S Buhimschi
INTRODUCTION: We performed RNA sequencing with the primary goal of discovering key placental villous trophoblast (VT) and decidua basalis (DB) transcripts differentially expressed in intra-amniotic infection (IAI)-induced preterm birth (PTB). METHODS: RNA was extracted from 15 paired VT and DB specimens delivered of women with: 1) spontaneous PTB in the setting of amniocentesis-proven IAI and histological chorioamnionitis (n = 5); 2) spontaneous idiopathic PTB (iPTB, n = 5); and 3) physiologic term pregnancy (n = 5)...
August 2016: Placenta
https://www.readbyqxmd.com/read/27411776/intra-amniotic-candida-albicans-infection-induces-mucosal-injury-and-inflammation-in-the-ovine-fetal-intestine
#8
Maria Nikiforou, Esmee M R Jacobs, Matthew W Kemp, Mathias W Hornef, Matthew S Payne, Masatoshi Saito, John P Newnham, Leon E W Janssen, Alan H Jobe, Suhas G Kallapur, Boris W Kramer, Tim G A M Wolfs
Chorioamnionitis is caused by intrauterine infection with microorganisms including Candida albicans (C.albicans). Chorioamnionitis is associated with postnatal intestinal pathologies including necrotizing enterocolitis. The underlying mechanisms by which intra-amniotic C.albicans infection adversely affects the fetal gut remain unknown. Therefore, we assessed whether intra-amniotic C.albicans infection would cause intestinal inflammation and mucosal injury in an ovine model. Additionally, we tested whether treatment with the fungistatic fluconazole ameliorated the adverse intestinal outcome of intra-amniotic C...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27403846/vaginal-fluid-il-6-concentrations-as-a-point-of-care-test-is-of-value-in-women-with-preterm-prom
#9
Ivana Musilova, Tomas Bestvina, Martina Hudeckova, Igor Michalec, Teresa Cobo, Bo Jacobsson, Marian Kacerovsky
BACKGROUND: Preterm prelabor rupture of membranes (PROM) is frequently complicated/accompanied by infection and inflammation in the amniotic cavity. A point-of-care determination of amniotic fluid interleukin-6 has been shown to be a potentially clinically useful approach to assess inflammatory status of the amniotic cavity. Amniocentesis in preterm PROM is not broadly used in clinical practice, and therefore a shift towards a non-invasive amniotic fluid sampling method is needed. OBJECTIVE: The first aim of this study was to evaluate the association between the point-of-care vaginal and amniotic fluid interleukin-6 concentrations in fresh unprocessed samples obtained simultaneously...
July 9, 2016: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/27402051/vaginal-fluid-il-6-concentrations-as-a-point-of-care-test-is-of-value-in-women-with-preterm-prom
#10
Ivana Musilova, Tomas Bestvina, Martina Hudeckova, Igor Michalec, Teresa Cobo, Bo Jacobsson, Marian Kacerovsky
BACKGROUND: Preterm prelabor rupture of membranes (PROM) is frequently complicated/accompanied by infection and inflammation in the amniotic cavity. A point-of-care determination of amniotic fluid interleukin-6 has been shown to be a potentially clinically useful approach to assess inflammatory status of the amniotic cavity. Amniocentesis in preterm PROM is not broadly used in clinical practice, and therefore a shift towards a non-invasive amniotic fluid sampling method is needed. OBJECTIVE: The first aim of this study was to evaluate the association between the point-of-care vaginal and amniotic fluid interleukin-6 concentrations in fresh unprocessed samples obtained simultaneously...
July 8, 2016: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/27378700/surface-functionality-affects-the-biodistribution-and-microglia-targeting-of-intra-amniotically-delivered-dendrimers
#11
Fan Zhang, Elizabeth Nance, Zhi Zhang, Venkatasai Jasty, Siva P Kambhampati, Manoj K Mishra, Irina Burd, Roberto Romero, Sujatha Kannan, Rangaramanujam M Kannan
Cerebral Palsy (CP) is a chronic childhood disorder with limited therapeutic options. Maternal intrauterine inflammation/infection is a major risk factor in the pathogenesis of CP. In pre-clinical models, dendrimer-based therapies are viable in postnatal period, attenuating inflammation and improving motor function in vivo. However, treatment to the mother, in the prenatal period, may provide the possibility of preventing/resolving inflammation at early stages. Towards this goal, we used a maternal intrauterine inflammation-induced rabbit model of CP to study fetal-maternal transport and neuroinflammation targeting of intra-amniotically administrated dendrimers with neutral/anionic surface functionality...
September 10, 2016: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/27312808/lipidomic-analysis-of-patients-with-microbial-invasion-of-the-amniotic-cavity-reveals-up-regulation-of-leukotriene-b4
#12
Krishna Rao Maddipati, Roberto Romero, Tinnakorn Chaiworapongsa, Piya Chaemsaithong, Sen-Lin Zhou, Zhonghui Xu, Adi L Tarca, Juan Pedro Kusanovic, Ricardo Gomez, Noppadol Chaiyasit, Kenneth V Honn
Bioactive lipids derived from the metabolism of polyunsaturated fatty acids are important mediators of the inflammatory response. Labor per se is considered a sterile inflammatory process. Intra-amniotic inflammation (IAI) due to microorganisms (i.e., intra-amniotic infection) or danger signals (i.e., sterile IAI) has been implicated in the pathogenesis of preterm labor and clinical chorioamnionitis at term. Early and accurate diagnosis of microbial invasion of the amniotic cavity (MIAC) requires analysis of amniotic fluid (AF)...
October 2016: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/27251223/evidence-for-participation-of-neutrophil-gelatinase-associated-lipocalin-matrix-metalloproteinase-9-ngal%C3%A2-mmp-9-complex-in-the-inflammatory-response-to-infection-in-pregnancies-complicated-by-preterm-birth
#13
Kara M Rood, Irina A Buhimschi, Katherine Rodewald Millen, Mert O Bahtiyar, Stephen Thung, Taryn Summerfield, Guomao Zhao, William Ackerman, Cynthia Shellhaas, Philip Samuels, Catalin S Buhimschi
PROBLEM: Neutrophil gelatinase-associated lipocalin (NGAL) is expressed in neutrophils and involved in innate immunity by sequestering iron. NGAL's ability to complex with matrix metalloproteinase-9 (MMP-9) and extend its gelatinolytic activity led us to investigate its role in pregnancies complicated by preterm birth (PTB) and intra-amniotic infection/inflammation (IAI). METHOD OF STUDY: We assayed the amniotic fluid (AF) levels of NGAL and MMP-9 in 308 women that had a clinically indicated amniocentesis and a normal pregnancy outcome or PTB...
August 2016: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/27217375/intrauterine-candida-albicans-infection-causes-systemic-fetal-candidiasis-with-progressive-cardiac-dysfunction-in-a-sheep-model-of-early-pregnancy
#14
Sarah J Stock, Olga Patey, Basky Thilaganathan, Scott White, Lucy L Furfaro, Matthew S Payne, Owen B Spiller, Andres Noé, Rory Watts, Sean Carter, Demelza J Ireland, Alan H Jobe, John P Newnham, Matthew W Kemp
INTRODUCTION: Several recent studies have identified a potential role for intrauterine Candida albicans in adverse pregnancy outcomes, including preterm birth. There is, however, a limited understanding of the impact of intrauterine candida infection on fetal well-being in early pregnancy. Using a sheep model of early pregnancy, the aims of this study were to determine (1) the ability of experimentally induced intrauterine C albicans to infect the fetus and (2) whether C albicans exposure in early pregnancy is associated with alterations in fetal cardiac function, as measured by spectral tissue Doppler imaging analysis of fetal cardiac function...
May 23, 2016: Reproductive Sciences
https://www.readbyqxmd.com/read/27159193/imbalance-of-amniotic-fluid-activin-a-and-follistatin-in-intraamniotic-infection-inflammation-and-preterm-birth
#15
John T Hardy, Irina A Buhimschi, Megan E McCarthy, Guomao Zhao, Christine A Laky, Lydia L Shook, Catalin S Buhimschi
CONTEXT: Microbial invasion of the amniotic fluid (AF) cavity stimulates an inflammatory response that involves activin-A, a pleiotropic mediator member of the TGFβ superfamily involved in connective tissue remodeling. The role of AF follistatin, a natural inhibitor of activin-A, in inflammation-induced preterm birth (PTB), has yet to be determined. OBJECTIVE: The objective of the study was to investigate the relationships between AF activin-A and follistatin in physiological gestation and in pregnancies complicated by PTB and to evaluate a possible role played by the activin-A-follistatin balance in processes leading to PTB and preterm premature rupture of membranes (PPROM)...
July 2016: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/27066004/a-new-potent-and-placenta-permeable-macrolide-antibiotic-solithromycin-for-the-prevention-and-treatment-of-bacterial-infections-in-pregnancy
#16
REVIEW
Jeffrey A Keelan, Matthew S Payne, Matthew W Kemp, Demelza J Ireland, John P Newnham
Intrauterine infection-inflammation is a major cause of early preterm birth and subsequent neonatal mortality and acute or long-term morbidity. Antibiotics can be administered in pregnancy to prevent preterm birth either prophylactically to women at high risk for preterm delivery, or to women with diagnosed intrauterine infection, prelabor rupture of membranes, or in suspected preterm labor. The therapeutic goals of each of these scenarios are different, with different pharmacological considerations, although effective antimicrobial therapy is an essential requirement...
2016: Frontiers in Immunology
https://www.readbyqxmd.com/read/27061307/gestational-age-is-more-important-for-short-term-neonatal-outcome-than-microbial-invasion-of-the-amniotic-cavity-or-intra-amniotic-inflammation-in-preterm-prelabor-rupture-of-membranes
#17
Adriano Rodríguez-Trujillo, Teresa Cobo, Irene Vives, Jordi Bosch, Marian Kacerovsky, David E Posadas, Martina A Ángeles, Eduard Gratacós, Bo Jacobsson, Montse Palacio
INTRODUCTION: The aim of this study was to evaluate, in women with preterm prelabor rupture of membranes (PPROM), the impact on short-term neonatal outcome of microbial invasion of the amniotic cavity (MIAC), intra-amniotic inflammation (IAI), and the microorganisms isolated in women with MIAC, when gestational age is taken into account. MATERIAL AND METHODS: Prospective cohort study. We included women with PPROM (22.0-34.0 weeks of gestation) with available information about MIAC, IAI and short-term neonatal outcome...
August 2016: Acta Obstetricia et Gynecologica Scandinavica
https://www.readbyqxmd.com/read/27054843/amniotic-fluid-rapid-biomarkers-are-associated-with-intra-amniotic-infection-in-preterm-pregnancies-regardless-of-the-membrane-status
#18
T Myntti, L Rahkonen, M Tikkanen, A Pätäri-Sampo, J Paavonen, V Stefanovic
OBJECTIVE: The objective of this study was to evaluate the association of amniotic fluid lactate dehydrogenase and glucose concentrations with microbial invasion of amniotic cavity and histologic chorioamnionitis before 37 pregnancy weeks in women with or without preterm premature rupture of membranes. STUDY DESIGN: Amniocentesis was performed on 70 women with suspected intra-amniotic infection. Standard biochemical methods, molecular microbiology and culture techniques were used...
August 2016: Journal of Perinatology: Official Journal of the California Perinatal Association
https://www.readbyqxmd.com/read/27035826/hydrogen-sulfide-delays-lps-induced-preterm-birth-in-mice-via-anti-inflammatory-pathways
#19
Weina Liu, Chen Xu, Xingji You, David M Olson, Sylvain Chemtob, Lu Gao, Xin Ni
A major cause of preterm labor in pregnant women is intra-amniotic infection, which is mediated by an inflammatory process. Hydrogen sulfide (H2S), a gaseous transmitter, has been implicated to be involved in inflammatory responses. We sought to investigate whether H2S affects infectious preterm birth using the mouse model of lipopolysaccharides (LPS)-induced preterm birth. Administration of LPS at 0.4 mg/kg with two injections intraperitoneally (i.p.) on gestational day 14.5 induced preterm labor. LPS significantly increased leukocyte infiltration in uterus, stimulated the expression of pro-inflammatory cytokines interleukin 1β (IL-1β), IL-6, tumor necrosis factor α (TNF-α), CCL2 and CXCL15 in myometrium...
2016: PloS One
https://www.readbyqxmd.com/read/27009041/inflammatory-response-of-human-gestational-membranes-to-ureaplasma-parvum-using-a-novel-dual-chamber-tissue-explant-system
#20
Lauren C Potts, Liping Feng, Patrick C Seed, Friederike L Jayes, Maragatha Kuchibhatla, Brian Antczak, Matthew K Nazzal, Amy P Murtha
Preterm premature rupture of membranes (PPROM) is often associated with intra-amniotic inflammation and infection. Current understanding of the pathogenesis of PPROM includes activation of pro-inflammatory cytokines and proteolytic enzymes leading to compromise of membrane integrity. The impact of exposure to bacterial pathogens, including Ureaplasma parvum, on gestational membranes is poorly understood. Our objective was to develop a dual-chamber system to characterize the inflammatory response of gestational membranes to U...
May 2016: Biology of Reproduction
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