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https://www.readbyqxmd.com/read/28823139/effect-of-single-nucleotide-polymorphism-on-the-total-number-of-piglets-born-per-parity-of-three-different-pig-breeds
#1
Kyoung-Tag Do, Soon-Woo Jung, Kyung-Do Park, Chong-Sam Na
Objective: To determine the effects of genomic breeding values (GBV) and single nucleotide polymorphisms (SNP) on the total number of piglets born (TNB) in 3 pig breeds (Berkshire, Landrace, and Yorkshire). Methods: After collecting genomic information (Porcine SNP Beadchip) and phenotypic TNB records for each breed, the effects of GBV and SNP were estimated by using single step best linear unbiased prediction (ssBLUP) method. Results: The heritability estimates for TNB in Berkshire, Landrace, and Yorkshire breeds were 0...
August 16, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28822995/determination-of-norflurazon-concentration-in-wheat-leaves-using-a-modified-quechers-method
#2
Kamil F Trzebuniak, Beata Mysliwa-Kurdziel
The aim of this analytical study was to develop and validate an easy-to-use method for measuring the actual level of norflurazon that accumulates in leaves. We amended the QuEChERS method, i.e. Quick, Easy, Cheap, Effective, Rugged, and Safe, which is widely used for pesticide and herbicide analysis in food, and usually combined with HPLC-MS detection. We adapted this method for the detection of norflurazon in leaves or leaf fragments and proposed a useful modification using of HPLC-UV detection. Reproducible retention times of 3...
August 19, 2017: Acta Biochimica Polonica
https://www.readbyqxmd.com/read/28822574/analysis-of-individual-and-total-microcystins-in-surface-water-by-on-line-preconcentration-and-desalting-coupled-to-liquid-chromatography-tandem-mass-spectrometry
#3
Gabriel Munoz, Sung Vo Duy, Audrey Roy-Lachapelle, Barry Husk, Sébastien Sauvé
A fast and high-throughput method is proposed for the determination of total microcystins (ΣMC) in environmental surface waters. After a 1-h Lemieux-von Rudloff oxidation step to yield the 2-methyl-3-methoxy-4-phenylbutyric acid (MMPB) moiety, samples were quenched, filtered, and directly analyzed. This was achieved via solid phase extraction (SPE) coupled on-line to ultra-high performance liquid chromatography electrospray ionization triple stage quadrupole mass spectrometry. The choice of on-line SPE settings was conducted using experimental designs...
August 5, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28821877/association-of-matrix-gla-protein-gene-rs1800801-rs1800802-rs4236-polymorphism-with-vascular-calcification-and-atherosclerotic-disease-a-meta-analysis
#4
Kaixiang Sheng, Ping Zhang, Weiqiang Lin, Jun Cheng, Jiawei Li, Jianghua Chen
Association between the MGP gene rs1800801, rs1800802, rs4236 polymorphisms and vascular calcification and atherosclerotic disease was inconsistent. To clarify precise association, we performed this meta-analysis. Medline, Embase and China Knowledge Resource Integrated Database were systematically searched through December 2016. A total of 23 case-control studies, consisting of 5280 cases and 5773 controls, were included. The overall results suggested that the -7A polymorphism was associated with an increased risk for vascular calcification and atherosclerotic disease in the recessive model (OR = 1...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821795/unconventional-fractional-quantum-hall-effect-in-bilayer-graphene
#5
Janusz Edward Jacak
Recent experimental progress in Hall measurements in bilayer graphene in the so-called open-face configuration of boron nitride encapsulated samples, together with the earlier technique of suspended samples, allows for precise observation of the fractional quantum Hall effect (FQHE) in all 4 subbands of the Lowest Landau level (with n = 0 and n = 1) and in the next LL subbands (with n = 2) in the bilayer system. Many newly observed FQHE features do not agree with a conventional model of composite fermions and reveal a different hierarchy in comparison to monolayer graphene or GaAs 2DEG...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28820460/determination-of-sphingosine-1-phosphate-in-human-plasma-using-liquid-chromatography-coupled-with-q-tof-mass-spectrometry
#6
Emmanuel Eroume-A Egom, Ross Fitzgerald, Rebecca Canning, Rebabonye B Pharithi, Colin Murphy, Vincent Maher
Evidence suggests that high-density lipoprotein (HDL) components distinct from cholesterol, such as sphingosine-1-phosphate (S1P), may account for the anti-atherothrombotic effects attributed to this lipoprotein. The current method for the determination of plasma levels of S1P as well as levels associated with HDL particles is still cumbersome an assay method to be worldwide practical. Recently, a simplified protocol based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the sensitive and specific quantification of plasma levels of S1P with good accuracy has been reported...
August 18, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28820278/does-additional-biceps-augmentation-improve-rotator-cuff-healing-and-clinical-outcomes-in-anterior-l-shaped-rotator-cuff-tears-clinical-comparisons-with-arthroscopic-partial-repair
#7
Yoon Sang Jeon, Juyeob Lee, Rag Gyu Kim, Young-Won Ko, Sang-Jin Shin
BACKGROUND: The repair of anterior L-shaped tears is usually difficult because of the lack of anterior rotator cuff tendon to cover the footprint. The biceps tendon is usually exposed from the retracted anterolateral corner of the torn tendon and can be easily used to augment rotator cuff repair. Hypothesis/Purpose: This study compared the clinical outcomes of the biceps augmentation technique with those of partial tendon repair for the arthroscopic treatment of large anterior L-shaped rotator cuff tears to evaluate the role of additional biceps augmentation in tendon healing...
August 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28820183/technique-of-retinal-gene-therapy-delivery-of-viral-vector-into-the-subretinal-space
#8
K Xue, M Groppe, A P Salvetti, R E MacLaren
PurposeSafe and reproducible delivery of gene therapy vector into the subretinal space is essential for successful targeting of the retinal pigment epithelium (RPE) and photoreceptors. The success of surgery is critical for the clinical efficacy of retinal gene therapy. Iatrogenic detachment of the degenerate (often adherent) retina in patients with hereditary retinal degenerations and small volume (eg, 0.1 ml) subretinal injections pose new surgical challenges.MethodsOur subretinal gene therapy technique involved pre-operative planning with optical coherence tomography (OCT) and autofluorescence (AF) imaging, 23 G pars plana vitrectomy, internal limiting membrane staining with Membrane Blue Dual (DORC BV, Zuidland, Netherlands), a two-step subretinal injection using a 41 G Teflon tipped cannula (DORC) first with normal saline to create a parafoveal bleb followed by slow infusion of viral vector via the same self-sealing retinotomy...
August 18, 2017: Eye
https://www.readbyqxmd.com/read/28820079/validation-of-a-system-for-monitoring-individual-feeding-and-drinking-behaviour-and-intake-in-young-cattle
#9
B R Oliveira, M N Ribas, F S Machado, J A M Lima, L F L Cavalcanti, M L Chizzotti, S G Coelho
The objective of this study was to validate an electronic system for monitoring individual feeding and drinking behaviour and intake developed for young cattle housed in group. A total of 35 Holstein-Gyr crossbred heifers (BW: 180±52 kg; age: 121.5±32.5 days), fitted with an ear tag containing a unique passive transponder, were distributed in three groups of 12, 12 and 11 animals per period and had free access to 12 electronic feed bins and two electronic water bins (Intergado® Ltd). The dimensions of feed and water bins, as well as the sensors position were appropriate for young cattle...
August 18, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28818830/high-speed-melting-analysis-the-effect-of-melting-rate-on-small-amplicon-microfluidic-genotyping
#10
Robert J Pryor, Joseph T Myrick, Robert A Palais, Scott O Sundberg, Jeanette Y Paek, Carl T Wittwer, Ivor T Knight
BACKGROUND: High-resolution DNA melting analysis of small amplicons is a simple and inexpensive technique for genotyping. Microfluidics allows precise and rapid control of temperature during melting. METHODS: Using a microfluidic platform for serial PCR and melting analysis, 4 targets containing single nucleotide variants were amplified and then melted at different rates over a 250-fold range from 0.13 to 32ü°C/s. Genotypes (n = 1728) were determined manually by visual inspection after background removal, normalization, and conversion to negative derivative plots...
August 17, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28818818/validation-of-a-smartphone-based-approach-to-in-situ-cognitive-fatigue-assessment
#11
Edward Price, George Moore, Leo Galway, Mark Linden
BACKGROUND: Acquired Brain Injuries (ABIs) can result in multiple detrimental cognitive effects, such as reduced memory capability, concentration, and planning. These effects can lead to cognitive fatigue, which can exacerbate the symptoms of ABIs and hinder management and recovery. Assessing cognitive fatigue is difficult due to the largely subjective nature of the condition and existing assessment approaches. Traditional methods of assessment use self-assessment questionnaires delivered in a medical setting, but recent work has attempted to employ more objective cognitive tests as a way of evaluating cognitive fatigue...
August 17, 2017: JMIR MHealth and UHealth
https://www.readbyqxmd.com/read/28818802/development-and-validation-of-a-gc-ms-method-for-the-determination-of-hydroxyzine-and-its-active-metabolite-cetirizine-in-whole-blood
#12
Maria Katselou, Sotiris Athanaselis, Panagiota Nikolaou, Artemisia Dona, Chara Spiliopoulou, Ioannis Papoutsis
A simple, rapid, sensitive and accurate gas chromatography-mass spectrometric method was developed and validated for the simultaneous determination of hydroxyzine and cetirizine in whole blood. Solid-phase extraction procedure using Bond Elut LRC Certify II columns was used for the isolation of hydroxyzine and cetirizine from 1mL whole blood followed by derivatization with a mixture of acetic anhydride:n-propanol (1:1, v/v). Limits of detection and quantification were 1.50 and 5.00ng/mL, respectively. The assay was linear within the concentration range of 5...
August 1, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28818564/validity-of-a-smartphone-protractor-to-measure-sagittal-parameters-in-adult-spinal-deformity
#13
William Aaron Kunkle, Michael Madden, Shannon Potts, Jeremy Fogelson, Stuart Hershman
BACKGROUND CONTEXT: Smartphones have become an integral tool in the daily life of healthcare professionals (Franko 2011). Their ease of use and wide availability often make smartphones the first tool surgeons use to perform measurements. This technique has been validated for certain orthopaedic pathologies (Shaw 2012, Quek 2014, Milanese 2014, Milani 2014), but never to assess sagittal parameters in adult spinal deformity. This study was designed to assess the validity, reproducibility, precision and efficiency of using a smartphone protractor application to measure sagittal parameters commonly measured in adult spinal deformity assessment and surgical planning...
August 14, 2017: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/28818328/deep-eutectic-solvent-based-ultrasound-assisted-dispersive-liquid-liquid-microextraction-coupled-with-high-performance-liquid-chromatography-for-the-determination-of-ultraviolet-filters-in-water-samples
#14
Huazi Wang, Lu Hu, Xinya Liu, Shujun Yin, Runhua Lu, Sanbing Zhang, Wenfeng Zhou, Haixiang Gao
In the present study, a simple and rapid sample preparation method designated ultrasound-assisted dispersive liquid-liquid microextraction based on a deep eutectic solvent (DES) followed by high-performance liquid chromatography with ultraviolet (UV) detection (HPLC-UVD) was developed for the extraction and determination of UV filters from water samples. The model analytes were 2,4-dihydroxybenzophenone (BP-1), benzophenone (BP) and 2-hydroxy-4-methoxybenzophenone (BP-3). The hydrophobic DES was prepared by mixing trioctylmethylammonium chloride (TAC) and decanoic acid (DecA)...
July 22, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28817233/evaluation-and-quantitative-analysis-of-11-compounds-in-morinda-officinalis-using-ultra-high-performance-liquid-chromatography-and-photodiode-array-detection-coupled-with-chemometrics
#15
Xiangsheng Zhao, Weijun Kong, Yakui Zhou, Jianhe Wei, Meihua Yang
Morinda officinalis (Rubiaceae) is a traditional Chinese medicine widely used for the treatment of impotence and osteoporosis in clinical therapy. In the present study, a rapid and simple ultra high performance liquid chromatography with photodiode array detection method was developed and validated for the simultaneous determination of 11 bioactive compounds in M. officinalis. This assay method was validated with respect to linearity (R(2)( ) > 0.9991), precision, repeatability, limit of detection, limit of quantification, and accuracy (with observed recovery rates between 94...
August 17, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28817196/technical-note-quantitative-analysis-of-ivim-diffusion-mri-using-total-variation-and-huber-penalty-function
#16
Esha Baidya Kayal, Devasenathipathy Kandasamy, Kedar Khare, Jayendra Tiru Alampally, Sameer Bakhshi, Raju Sharma, Amit Mehndiratta
PURPOSE: Quantitative analysis in Intra-voxel Incoherent Motion (IVIM) imaging commonly uses voxel-wise estimation of the bi-exponential model, which might not be reliable for clinical interpretation. Improving model fitting performance and qualitative and quantitative parametric estimation, two novel methodologies are proposed here. METHODS: Five IVIM analyses methodologies: i) Bi-Exponential (BE) model, ii) Segmented BE method with 2-parameter fitting (BEseg-2), iii) Segmented BE method with 1-parameter fitting (BEseg-1), iv) BE with adaptive Total Variation Penalty function (BE+TV) and v) BE with adaptive Huber Penalty function (BE+HPF) were evaluated...
August 17, 2017: Medical Physics
https://www.readbyqxmd.com/read/28816646/chronic-cigarette-smoke-mediated-global-changes-in-lung-mucoepidermoid-cells-a-phosphoproteomic-analysis
#17
Hitendra S Solanki, Jayshree Advani, Aafaque Ahmad Khan, Aneesha Radhakrishnan, Nandini A Sahasrabuddhe, Sneha M Pinto, Xiaofei Chang, Thottethodi Subrahmanya Keshava Prasad, Premendu Prakash Mathur, David Sidransky, Harsha Gowda, Aditi Chatterjee
Proteomics analysis of chronic cigarette smoke exposure is a rapidly emerging postgenomics research field. While smoking is a major cause of lung cancer, functional studies using proteomics approaches could enrich our mechanistic understanding of the elusive lung cancer global molecular signaling and cigarette smoke relationship. We report in this study on a stable isotope labeling by amino acids in cell culture-based quantitative phosphoproteomic analysis of a human lung mucoepidermoid carcinoma cell line, H292 cells, chronically exposed to cigarette smoke...
August 2017: Omics: a Journal of Integrative Biology
https://www.readbyqxmd.com/read/28816438/interfacial-mechanisms-of-water-vapor-sorption-into-cellulose-nanofibril-films-as-revealed-by-quantitative-models
#18
Minna Hakalahti, Marco Faustini, Cédric Boissière, Eero Kontturi, Tekla Tammelin
Humidity is an efficient instrument for facilitating changes in local architectures of 2-dimensional surfaces assembled from nanoscaled biomaterials. Here, complementary surface-sensitive methods are used to collect explicit and precise experimental evidence on the water vapor sorption into (2,2,6,6-tetramethylpiperidin-1-yl)oxyl (TEMPO) oxidized cellulose nanofibril (CNF) thin film over the relative humidity (RH) range from 0 to 97%. Changes in thickness and mass of the film due to water vapor uptake are tracked using Spectroscopic Ellipsometry and Quartz Crystal Microbalance with Dissipation monitoring, respectively...
August 17, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28816411/a-stereovision-model-applied-in-bio-micromanipulation-system-based-on-stereo-light-microscope
#19
Yuezong Wang
A bio-micromanipulation system is designed for manipulating micro-objects with a length scale of tens or hundreds of microns based on stereo light microscope. The world coordinate reconstruction of points on the surface of micro-objects is an important goal for the micromanipulation. Traditional pinhole camera model is applied widely in macrocomputer vision. However, this model will output bad data with remarkable error if it is directly used to reconstruct three-dimensional world coordinates for stereo light microscope...
August 17, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28816315/a-highly-selective-and-sensitive-fluorescent-nanosensor-for-dopamine-based-on-formate-bridged-tb-iii-complex-and-silver-nanoparticles
#20
Huihui Li, Jin Shen, Rongwei Cui, Chongmei Sun, Yanyan Zhao, Xia Wu, Na Li, Bo Tang
Highly selective determination of dopamine (DA) over other catecholamines is an urgent need for the precise diagnosis and therapy of DA related diseases. Herein, a new formate-bridged Tb(iii)-complex and silver nanoparticles (AgNPs) enhanced fluorescent nanosensor was constructed. HCOO(-) acted as a co-ligand of Tb(iii) and also as a linker between the Tb(iii) complex and AgNPs and more readily combined with the primary amine of DA than with epinephrine (EP). The formate-bridged action strengthened AgNPs-based surface enhanced fluorescence of the Tb(3+)-DA complex and improved the selectivity towards DA...
August 17, 2017: Analyst
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