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https://www.readbyqxmd.com/read/28734271/development-and-validation-of-sensitive-lc-ms-ms-method-for-the-quantification-of-suvn-502-and-its-metabolite-and-its-application-for-first-in-human-pharmacokinetic-study
#1
Ramakrishna Nirogi, Devender Reddy Ajjala, Raghupathi Aleti, Lakshmiprasanna Rayapati, Hanumanth Rao Pantangi, Rajesh Kumar Boggavarapu, Naga Surya Prakash Padala
A sensitive and rapid LC-MS/MS method was developed and validated for the quantification of SUVN-502 and M1 of SUVN-502, a 5-HT6 receptor antagonist for the treatment of dementia associated with Alzheimer's disease. Following solid-phase extraction, SUVN-502 and M1 of SUVN-502 and IS were eluted with 10mM ammonium acetate (pH 4.0) and acetonitrile using a rapid gradient program on reverse phase column. Multiple reaction monitoring mode was used to monitor the respective transitions of m/z 478.2→377.7 for SUVN-502 and m/z 464...
April 14, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28734159/development-of-a-lc-ms-ms-method-for-the-determination-of-ckd-712-in-rat-plasma-application-to-a-pharmacokinetic-study-in-rats
#2
Jung-Woo Chae, Hwi-Yeol Yun, Han Young Eom, Eun Ju Jeong, Tae-Sung Koo, Kwang-Il Kwon, Jong-Hwa Lee
CKD-712 is a potential treatment for sepsis, as it exhibits protective effects against lipopolysaccharide-mediated platelet aggregation, inducible nitric oxide synthase expression, and cecum-ligation puncture-induced septic mortality in mice. In this study, we develop a rapid and sensitive LC-MS/MS method for determining CKD-712 in rat plasma. CKD-712 and papaverine hydrochloride (an internal standard) were analyzed using an LC-MS/MS system consisting of an Agilent HPLC system (HP-1100) equipped with an Atlantis HILIC Silica (2...
July 8, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28731812/3d-printed-visceral-aneurysm-models-based-on-ct-data-for-simulations-of-endovascular-embolization-evaluation-of-size-and-shape-accuracy
#3
Eisuke Shibata, Hidemasa Takao, Shiori Amemiya, Kuni Ohtomo
OBJECTIVE: The objective of this study is to verify the accuracy of 3D-printed hollow models of visceral aneurysms created from CT angiography (CTA) data, by evaluating the sizes and shapes of aneurysms and related arteries. SUBJECTS AND METHODS: From March 2006 to August 2015, 19 true visceral aneurysms were embolized via interventional radiologic treatment provided by the radiology department at our institution; aneurysms with bleeding (n = 3) or without thin-slice (< 1 mm) preembolization CT data (n = 1) were excluded...
August 2017: AJR. American Journal of Roentgenology
https://www.readbyqxmd.com/read/28731212/cross-validation-of-commercial-enzyme-linked-immunosorbent-assay-and-radioimmunoassay-for-porcine-c-peptide-concentration-measurements-in-non-human-primate-serum
#4
Sarah C Gresch, Lucas A Mutch, Jody L Janecek, Rebecca L Hegstad-Davies, Melanie L Graham
BACKGROUND: C-peptide concentration is widely used as a marker of insulin secretion and is especially relevant in evaluating islet graft function following transplantation, because its measurement is not confounded by the presence of exogenous insulin. To address the shortage of human islet donors, the use of porcine islets has been proposed as a possible solution and the stringent pig-to-non-human primate (NHP) model is often the most relevant for pre-clinical evaluation of the potential for diabetes reversal resulting from an islet xenograft...
July 21, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/28729835/uplc-esi-ms-ms-and-hptlc-method-for-quantitative-estimation-of-cytotoxic-glycosides-and-aglycone-in-bioactivity-guided-fractions-of-solanum-nigrum-l
#5
Karishma Chester, Sarvesh Paliwal, Washim Khan, Sayeed Ahmad
Solanum nigrum L., is traditionally used for the management of the various liver disorders. Investigating the effect of polarity based fractionation of S. nigrum for its hepatoprotective effect on Hep G2 cells in vitro to provide base of its activity by quantifying in steroidal glycosides responsible for hepatoprotective potential. A new UPLC-ESI-MS/MS method following a high performance thin layer chromatography (HPTLC) has been developed and validated for quantification of steroidal glycosides and aglycone (solasonine, solamargine, and solasodine, respectively)...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28728993/value-of-tracking-biopsy-in-men-undergoing-active-surveillance-of-prostate-cancer
#6
Edward Chang, Tonye A Jones, Shyam Natarajan, Devi Sharma, Demetrios Simopoulos, Daniel J Margolis, Jiaoti Huang, Frederick J Dorey, Leonard S Marks
PURPOSE: To compare the upgrading rate obtained by re-sampling precise spots of prostate cancer (tracking biopsy) vs conventional systematic re-sampling, during follow-up of; men in active surveillance. MATERIALS AND METHODS: Subjects were all 352 men, from 2009 to 2017, with Gleason 3+3 (n=268) or Gleason 3+4 (n=84) prostate cancer at initial MRI/ultrasound fusion biopsy and who subsequently had a second fusion biopsy. At first biopsy session, all men underwent 12-core systematic biopsies and, when MRI-visible lesions were present, targeted biopsies...
July 17, 2017: Journal of Urology
https://www.readbyqxmd.com/read/28728205/-predictability-of-postoperative-pain-and-satisfaction-how-precise-are-we-and-what-is-the-role-of-professional-experience
#7
Ulf Krister Hofmann, Cosima Dobler, Marco Gesicki, Torsten Kluba, Sebastian Scheidt
Background The indication for surgery is justified by an expected improvement for the patient. To evaluate the probability and extent of individual postoperative patient benefit, the surgeon needs to elaborate numerous parameters of potential relevance for the outcome beyond his key competence, that is the technical dimension of the operation. Despite the highest medical standards, individual postoperative satisfaction with surgery is highly variable, even in cases with a technically good result. The aim of the present study was to investigate the individual predictability of postoperative pain and satisfaction in patients with elective musculoskeletal surgery...
July 20, 2017: Zeitschrift Für Orthopädie und Unfallchirurgie
https://www.readbyqxmd.com/read/28728168/lung-cancer-prognostic-index-a-risk-score-to-predict-overall-survival-after-the-diagnosis-of-non-small-cell-lung-cancer
#8
Marliese Alexander, Rory Wolfe, David Ball, Matthew Conron, Robert G Stirling, Benjamin Solomon, Michael MacManus, Ann Officer, Sameer Karnam, Kate Burbury, Sue M Evans
INTRODUCTION: Non-small-cell lung cancer outcomes are poor but heterogeneous, even within stage groups. To improve prognostic precision we aimed to develop and validate a simple prognostic model using patient and disease variables. METHODS: Prospective registry and study data were analysed using Cox proportional hazards regression to derive a prognostic model (hospital 1, n=695), which was subsequently tested (Harrell's c-statistic for discrimination and Cox-Snell residuals for calibration) in two independent validation cohorts (hospital 2, n=479 and hospital 3, n=284)...
July 20, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28727118/technical-note-assessment-of-an-alternative-technique-for-measuring-body-temperature-in-pigs
#9
A Petry, W McGilvray, A R Rakhshandeh, A Rakhshandeh
Core body temperature (CBT) is one of the main vital signs that is used to evaluate the health status of pigs. The most common and feasible method for assessing CBT in pigs is rectal temperature (RT). Obtaining RT is stressful for animals, may generate inaccurate results, and has the risk of spreading disease. Infrared imaging (IR) thermography of the body of pigs may be a safer and less stressful alternative to RT. The objective of the current study was to evaluate the efficacy of using IR as an alternative for monitoring CBT in pigs...
July 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28727107/national-beef-quality-audit-2016-survey-of-carcass-characteristics-through-instrument-grading-assessments
#10
C A Boykin, L C Eastwood, M K Harris, D S Hale, C R Kerth, D B Griffin, A N Arnold, J D Hasty, K E Belk, D R Woerner, R J Delmore, J N Martin, D L VanOverbeke, G G Mafi, M M Pfeiffer, T E Lawrence, T J McEvers, T B Schmidt, R J Maddock, D D Johnson, C C Carr, J M Scheffler, T D Pringle, A M Stelzleni, J Gottlieb, J W Savell
The instrument grading assessment portion of the National Beef Quality Audit (NBQA) - 2016 allows the unique opportunity to evaluate beef carcass traits over the course of a year. One week of instrument grading data was collected each month from 5 beef processing corporations encompassing 18 facilities from January 2016 through December 2016 ( = 4,544,635 carcasses). Mean USDA yield grade (YG) was 3.1 with 1.37 cm fat thickness (FT), 88.9 cm LM area, 393.6 kg HCW, and 2.1% KPH. Frequency distribution of USDA YG was 9...
July 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28727018/evaluation-of-electrical-broad-bandwidth-impedance-spectroscopy-as-a-tool-for-body-composition-measurement-in-cows-in-comparison-with-body-measurements-and-the-deuterium-oxide-dilution-method
#11
C T Schäff, U Pliquett, A Tuchscherer, R Pfuhl, S Görs, C C Metges, H M Hammon, C Kröger-Koch
Body fatness and degree of body fat mobilization in cows vary enormously during their reproduction cycle and influence energy partitioning and metabolic adaptation. The objective of the study was to test bioelectrical impedance spectroscopy (BIS) as a method for predicting fat depot mass (FDM), in living cows. The FDM is defined as the sum of subcutaneous, omental, mesenteric, retroperitoneal, and carcass fat mass. Bioelectrical impedance spectroscopy is compared with the prediction of FDM from the deuterium oxide (DO) dilution method and from body conformation measurements...
May 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28726286/a-new-sample-preparation-and-separation-combination-for-the-precise-accurate-and-simultaneous-determination-of-uracil-and-dihydrouracil-in-human-plasma-by-reversed-phase-hplc
#12
Wen Pan, Yuandong Li, Yan Feng, Fan Yang, Haizhou Liu
We have developed an efficient procedure and detection method using reversed-phase high-performance liquid chromatography for the simultaneous measurement of uracil and dihydrouracil in human plasma. The procedure, based on chromatographic conditions and sample preparation, was optimized and validated. Optimization of the sample preparation included of deproteinization, extraction and clean-up. A new sample preparation method which results in an improved extraction yield of analytes and significantly reduced interference at low-wavelength UV detection was developed...
July 20, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28726136/robotic-versus-laparoscopic-stapling-during-robotic-roux-en-y-gastric-bypass-surgery-a-case-matched-analysis-of-costs-and-clinical-outcomes
#13
Monika E Hagen, Minoa K Jung, Jassim Fakhro, Nicolas C Buchs, Leo Buehler, Jona M Mendoza, Philippe Morel
BACKGROUND: The purpose of this analysis is to compare the robotic EndoWrist Stapling System (EWSS) 45 mm (Intuitive Surgical Inc. Sunnyvale, CA, USA) and the ECHELON FLEX(™) ENDOPATH(®) Staplers (EFES) 60 mm (Ethicon, Cincinnati, OH, USA) for gastric pouch formation during robotic gastric bypass surgery. METHODS: Patients who underwent robotic gastric bypass surgery with stapling using EWSS were matched with patients who underwent the same procedure with the EFES...
July 19, 2017: Surgical Endoscopy
https://www.readbyqxmd.com/read/28725409/development-of-methane-conversion-factor-models-for-zebu-beef-cattle-fed-low-quality-crop-residues-and-by-products-in-tropical-regions
#14
Chatchai Kaewpila, Kritapon Sommart
The enteric methane conversion factor (Ym) is an important country-specific value for the provision of precise enteric methane emissions inventory reports. The objectives of this meta-analysis were to develop and evaluate the empirical Ym models for the national level and the farm level for tropical developing countries according to the IPCC's categorization. We used datasets derived from 18 in vivo feeding experiments from 1999 to 2015 of Zebu beef cattle breeds fed low-quality crop residues and by-products...
October 2016: Ecology and Evolution
https://www.readbyqxmd.com/read/28725212/neurochemical-systems-of-the-retina-involved-in-the-control-of-movement
#15
Gregory L Willis, Christopher B Freelance
Recent studies have revealed that the retina may exert control over deep brain function and may be importantly involved in the etiology, progression, and treatment of disorders such as Parkinson's disease (PD). While such a concept is uncharted territory and even less is known about the mechanism by which this might be achieved, this study was undertaken to determine how retinal dopamine (DA), serotonin (5-HT), and melatonin (MEL) neurotransmitter systems might be involved in the control of movement in their own right...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28723806/the-serum-pepsinogen-levels-for-risk-assessment-of-gastric-neoplasms-new-proposal-from-a-case-control-study-in-korea
#16
Jun-Hyung Cho, Seong Ran Jeon, Hyun Gun Kim, So-Young Jin, Suyeon Park
To decrease the gastric cancer related mortality rate, endoscopic screening is widely performed in Korea. However, a precise method for identifying those at a high risk of gastric neoplasms has not been established. This study aims to evaluate serum pepsinogen (PG) levels for risk assessment of gastric neoplasms. Between August 2014 and March 2016, a total of 398 subjects, including 87 with gastric neoplasms, were enrolled in this study. On the basis of the serum PG I/II ratio, the enrolled subjects were classified into 4 groups: group A, PG I/II ratio >4; group B, >3 and ≤4; group C, >2 and ≤3; group D, ≤2...
July 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28723652/a-patient-derived-orthotopic-xenograft-pdox-mouse-model-of-a-cisplatinum-resistant-osteosarcoma-lung-metastasis-that-was-sensitive-to-temozolomide-and-trabectedin-implications-for-precision-oncology
#17
Kentaro Igarashi, Takashi Murakami, Kei Kawaguchi, Tasuku Kiyuna, Kentaro Miyake, Yong Zhang, Scott D Nelson, Sarah M Dry, Yunfeng Li, Jane Yanagawa, Tara A Russell, Arun S Singh, Hiroyuki Tsuchiya, Irmina Elliott, Fritz C Eilber, Robert M Hoffman
In the present study, we evaluated the efficacy of trabectedin (TRAB) and temozolomide (TEM) compared to cisplatinum (CDDP) on a patient-derived orthotopic xenogrraft (PDOX) of a lung-metastasis from an osteosarcoma of a patient who failed CDDP therapy. Osteosarcoma resected from the patient was implanted orthotopically in the distal femur of mice to establish PDOX models which were randomized into the following groups when tumor volume reached approximately 100 mm3: G1, control without treatment; G2, CDDP (6 mg/kg, intraperitoneal injection, weekly, for 2 weeks); G3, TRAB (0...
July 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/28723274/identifying-voxels-at-risk-for-progression-in-glioblastoma-based-on-dosimetry-physiologic-and-metabolic-mri
#18
Mekhail Anwar, Annette M Molinaro, Olivier Morin, Susan M Chang, Daphne A Haas-Kogan, Sarah J Nelson, Janine M Lupo
Despite the longstanding role of radiation in cancer treatment and the presence of advanced, high-resolution imaging techniques, delineation of voxels at-risk for progression remains purely a geometric expansion of anatomic images, missing subclinical disease at risk for recurrence while treating potentially uninvolved tissue and increasing toxicity. This remains despite the modern ability to precisely shape radiation fields. A striking example of this is the treatment of glioblastoma, a highly infiltrative tumor that may benefit from accurate identification of subclinical disease...
July 19, 2017: Radiation Research
https://www.readbyqxmd.com/read/28722817/dispersive-solid-phase-extraction-for-the-determination-of-trace-organochlorine-pesticides-in-apple-juices-using-reduced-graphene-oxide-coated-with-zno-nanocomposites-as-sorbent
#19
Ting Sun, Hefeng Sun, Feng Zhao
In this work, reduced graphene oxide coated with ZnO nanocomposites was used as an efficient sorbent of dispersive solid-phase extraction and successfully applied to extraction of organochlorine pesticides from apple juice followed by gas chromatography with mass spectrometry. Several experimental parameters affecting the extraction efficiencies, including the amount of adsorbent, extraction time, and the pH of the sample solution, as well as the type and volume of eluent solvent, were investigated and optimized...
July 19, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28722029/effects-of-procedure-upright-equilibrium-time-sex-and-bmi-on-the-precision-of-body-fluid-measurements-using-bioelectrical-impedance-analysis
#20
S Thurlow, G Taylor-Covill, P Sahota, B Oldroyd, K Hind
BACKGROUND/OBJECTIVES: Extensive work has addressed the validity of bioimpedance (BIA) measurements and the effect of posture on fluid homeostasis. However, limited research has investigated effects of subject preparation. This study aimed to determine the precision of total body water (TBW) and extracellular water (ECW) measurements using a stand-on multifrequency BIA (MFBIA seca mBCA 514/515), in three pre-test procedures: supine, sitting, and following walking, with specific reference to the influence of sex and body mass index (BMI)...
July 19, 2017: European Journal of Clinical Nutrition
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