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https://www.readbyqxmd.com/read/29232755/-effect-of-the-age-related-corneal-elasticity-on-applanation-tonometry
#1
Karsten Sperlich, Stephan Reiß, Sebastian Bohn, Heinrich Stolz, Rudolf F Guthoff, Anselm Jünemann, Oliver Stachs
Elevated intraocular pressure (IOP) is accepted to be one important criterion for glaucoma and is usually measured by applanation or rebound tonometry. The individual uncertainty due to central cornea thickness (CCT) is thereby corrected, while the error induced by age-related elastic modulus (EM) change of the cornea is ignored. To investigate its influence on IOP measurement, we derive a model including also the elastic modulus. Our approach is based on known equations from experimental physics and several assumptions being justified in this paper...
December 2017: Klinische Monatsblätter Für Augenheilkunde
https://www.readbyqxmd.com/read/29232752/simultaneous-determination-of-rosuvastatin-rosuvastatin-5%C3%A2-s-lactone-and-n-desmethyl-rosuvastatin-in-human-plasma-by-uplc-ms-ms-and-its-application-to-clinical-study
#2
Xue Bai, Xi-Pei Wang, Guo-Dong He, Bin Zhang, Min Huang, Jia-Li Li, Shi-Long Zhong
Objective A rapid and sensitive ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) assay was developed and validated for the simultaneous quantification of rosuvastatin (RST), rosuvastatin-5 S-lactone (RSTL), and N-desmethyl rosuvastatin (DM-RST) in human plasma. Methods Sample was prepared by liquid-liquid extraction with ethyl acetate from 100 μL acidulated buffered plasma. Then analytes were chromatographically separated using an Acquity UPLC HSS T3 column (3.0 mm×100 mm, 1...
December 12, 2017: Drug Research
https://www.readbyqxmd.com/read/29232607/simultaneous-uplc-ms-ms-analysis-of-two-stable-isotope-labeled-versions-of-sucrose-in-mouse-plasma-and-brain-samples-as-markers-of-blood-brain-barrier-permeability-and-brain-vascular-space
#3
Ekram Ahmed Chowdhury, Faleh Alqahtani, Raktima Bhattacharya, Reza Mehvar, Ulrich Bickel
Blood Brain Barrier (BBB) permeability is frequently compromised in the course of diseases affecting the central nervous system (CNS). Sucrose is a low molecular weight, hydrophilic marker with slow permeability at the naive BBB and therefore one of the widely used indicators of barrier integrity. Our laboratory recently developed a highly sensitive UPLC-MS/MS method for stable isotope labeled [13C12]sucrose in biological matrices. Correction of total brain concentration for contribution of intravascular space is required in such experiments in order to accurately measure BBB permeability, and it is often accomplished by vascular perfusion with buffer solutions prior to brain sampling...
December 6, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29232606/microwave-assisted-synthesis-for-a2e-and-development-of-lc-esi-ms-method-for-quantification-of-ocular-bisretinoids-in-human-retina
#4
A Kotnala, S Senthilkumari, N Halder, A Kumar, T Velpandian
PURPOSE: To develop a microwave assisted method for the rapid synthesis of A2E and also to develop a method to quantify N-retinylidene-N-retinylethanolamine(A2E), all-trans retinal dimer (ATRD), A2-glycerophospho ethanolamine (A2GPE), dihydropyridine phosphatidyl ethanolamine (A2DHPE) and monofuran A2E (MFA2E) in age matched retina. METHODS: The development of microwave assisted synthesis of A2E, its purification and characterization for its utility in quantification in human retina...
November 26, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29231241/population-based-incidence-and-melanoma-specific-survival-of-cutaneous-malignant-melanoma-in-a-colombian-population-2000-2009
#5
Evy Reyes, Claudia Uribe, Esther de Vries
BACKGROUND: Demographic, clinical, and morphological characteristics of cutaneous melanoma are unknown for the Colombian population. We aim to provide these characteristics as well as population-based incidence and survival data. METHODS: All patients with an invasive cutaneous melanoma diagnosed in the period 2000-2009 registered in the population-based cancer registry of the metropolitan area of Bucaramanga were included for analysis (n = 169). Age-standardized incidence rates were calculated and melanoma-specific and overall survival estimated with follow-up until June 9, 2016, using Kaplan-Meier methodology, stratifying for gender, anatomical localization, and type of affiliation to the social security system...
December 12, 2017: International Journal of Dermatology
https://www.readbyqxmd.com/read/29231048/development-of-a-computerized-adaptive-test-for-anxiety-based-on-the-dutch-flemish-version-of-the-promis-item-bank
#6
Gerard Flens, Niels Smits, Caroline B Terwee, Joost Dekker, Irma Huijbrechts, Philip Spinhoven, Edwin de Beurs
We used the Dutch-Flemish version of the USA PROMIS adult V1.0 item bank for Anxiety as input for developing a computerized adaptive test (CAT) to measure the entire latent anxiety continuum. First, psychometric analysis of a combined clinical and general population sample ( N = 2,010) showed that the 29-item bank has psychometric properties that are required for a CAT administration. Second, a post hoc CAT simulation showed efficient and highly precise measurement, with an average number of 8.64 items for the clinical sample, and 9...
December 1, 2017: Assessment
https://www.readbyqxmd.com/read/29231019/-simultaneous-quantitative-analysis-of-koumine-gelsemine-and-gelsenicine-in-biological-samples-by-lc-ms-ms
#7
S J Ji, W Liu
OBJECTIVES: To establish a LC-MS/MS method which is accurate and sensitive for determination of koumine, gelsemine, and gelsenicine in biological samples and to verify the method. METHODS: Strychnine was used as internal standard. Analytes in blood, urine and liver with 1% sodium hydroxide solution were extracted by ethyl acetate. Chromatographic separation was achieved on a ZORBAX SB-C₁₈ column (150 mm×2.1 mm, 5 μm), and gradient elution was performed with the buffer solution of methanol-20 mmol/L ammonium acetate (including 0...
April 2017: Fa Yi Xue za Zhi
https://www.readbyqxmd.com/read/29230366/comparison-between-radial-artery-tonometry-pulse-analyzer-and-pulsed-doppler-echocardiography-derived-hemodynamic-parameters-in-cardiac-surgery-patients-a-pilot-study
#8
Rashad Zayat, Andreas Goetzenich, Ju-Yeon Lee, HeeJung Kang, So-Hyun Jansen-Park, Thomas Schmitz-Rode, Giulia Musetti, Heike Schnoering, Rüdiger Autschbach, Nima Hatam, Ali Aljalloud
Background: Bedside non-invasive techniques, such as radial artery tonometry, to estimate hemodynamic parameters have gained increased relevance as an attractive alternative and efficient method to measure hemodynamics in outpatient departments. For our pilot study, we sought to compare cardiac output (CO), and stroke volume (SV) estimated from a radial artery tonometry blood pressure pulse analyzer (BPPA) (DMP-Life, DAEYOMEDI Co., Gyeonggi-do, South Korea) to pulsed-wave Doppler (PWD) echocardiography derived parameters...
2017: PeerJ
https://www.readbyqxmd.com/read/29230178/human-cardiac-31p-mr-spectroscopy-at-3-tesla-cannot-detect-failing-myocardial-energy-homeostasis-during-exercise
#9
Adrianus J Bakermans, Jason N Bazil, Aart J Nederveen, Gustav J Strijkers, S Matthijs Boekholdt, Daniel A Beard, Jeroen A L Jeneson
Phosphorus-31 magnetic resonance spectroscopy (31P-MRS) is a unique non-invasive imaging modality for probing in vivo high-energy phosphate metabolism in the human heart. We investigated whether current 31P-MRS methodology would allow for clinical applications to detect exercise-induced changes in (patho-)physiological myocardial energy metabolism. Hereto, measurement variability and repeatability of three commonly used localized 31P-MRS methods [3D image-selected in vivo spectroscopy (ISIS) and 1D ISIS with 1D chemical shift imaging (CSI) oriented either perpendicular or parallel to the surface coil] to quantify the myocardial phosphocreatine (PCr) to adenosine triphosphate (ATP) ratio in healthy humans (n = 8) at rest were determined on a clinical 3 Tesla MR system...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29229406/validation-of-single-plane-fluoroscopy-and-2d-3d-shape-matching-for-quantifying-shoulder-complex-kinematics
#10
Rebekah L Lawrence, Arin M Ellingson, Paula M Ludewig
Fluoroscopy and 2D/3D shape-matching has emerged as the standard for non-invasively quantifying kinematics. However, its accuracy has not been well established for the shoulder complex when using single-plane fluoroscopy. The purpose of this study was to determine the accuracy of single-plane fluoroscopy and 2D/3D shape-matching for quantifying full shoulder complex kinematics. Tantalum markers were implanted into the clavicle, humerus, and scapula of four cadaveric shoulders. Biplane radiographs were obtained with the shoulder in five humerothoracic elevation positions (arm at the side, 30°, 60°, 90°, maximum)...
December 8, 2017: Medical Engineering & Physics
https://www.readbyqxmd.com/read/29229332/determination-of-household-and-industrial-chemicals-personal-care-products-and-hormones-in-leafy-and-root-vegetables-by-liquid-chromatography-tandem-mass-spectrometry
#11
Irene Aparicio, Julia Martín, Concepción Abril, Juan Luis Santos, Esteban Alonso
A multiresidue method has been developed for the determination of emerging pollutants in leafy and root vegetables. Selected compounds were 6 perfluoroalkyl compounds (5 perfluorocarboxylic acids and perfluorooctanesulfonic acid), 3 non-ionic surfactants (nonylphenol and nonylphenolethoxylates), 8 anionic surfactants (4 alkylsulfates and 4 linear alkylbenzene sulfonates), 4 preservatives (parabens), 2 biocides (triclosan and triclocarban), 2 plasticizers (bisphenol A and di-(2-ethylhexyl)phthalate), 6 UV-filters (benzophenones) and 4 hormones...
December 7, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29229322/-study-of-correlation-between-radiological-aspect-of-pulmonary-hydatid-cyst-and-postoperative-course
#12
W Abid, A Hentati, J Jdidi, Z Chaari, H Ben Jemaa, N Elleuch, J Dammak, I Frikha
Pulmonary Hydatid Cyst (PHC) may represent a real therapeutic challenge. Surgery remains the treatment of choice and postoperative course may be complicated in some cases. Several factors can be involved like the cyst's size and location. We aimed to study the existence of correlation between the radiological aspect of the PHC and the postoperative course through a retrospective study including 267 patients. Different radiological aspect of PHC found on the X-ray and/or computed tomography of the chest were classified according to Zidi et al...
December 8, 2017: Revue de Pneumologie Clinique
https://www.readbyqxmd.com/read/29228873/dehydroacetic-acid-in-cheese-and-cheese-coating-results-of-official-control-in-italy
#13
Monica Scordino, Francesco Lazzaro, Marco A Borzì, Leonardo Sabatino, Pasqualino Traulo, Giacomo Gagliano
This paper reports the occurrence of dehydroacetic acid in cheese and cheese coatings collected in Italy during Agricultural Ministry Official control. Dehydroacetic acid is an antimicrobial substance not allowed to be used in EU countries as a food additive, with unknown effects on human health. Dehydroacetic acid was measured by a validated HPLC method according to Commission Decision 2002/657/EC criteria in terms of specificity, linearity, precision and accuracy, limit of detection and limit of quantification...
December 11, 2017: Food Additives & Contaminants. Part B, Surveillance
https://www.readbyqxmd.com/read/29227544/a-fast-and-accurate-isotope-dilution-gc-it-ms-ms-method-for-determination-of-eugenol-in-different-tissues-of-fish-application-to-a-depletion-study-in-mandarin-fish
#14
Yongtao Liu, Xiaohui Ai, Le Li, Jincheng Li, Hong Yang
An accurate, rapid and effective method was established for determination of eugenol in plasma, muscle, skin, liver, kidney and gill of fish using gas chromatography-ion trap tandem mass spectrometry (GC-IT-MS/MS). Samples of muscle, skin, liver, kidney and gill were prepared using the modified QuEChERS (quick, easy, cheap, effective, rugged and safe) procedure, and plasma sample was prepared by a liquid-liquid extraction procedure. Eugenol was monitored in less than 7 min using an electron-ionization source (EI) in MS/MS mode and quantified by internal standard (IS) of eugenol-d3 ...
December 11, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/29227348/quantitative-pet-ct-in-clinical-practice-assessing-the-agreement-of-pet-tumor-indices-using-different-clinical-reading-platforms
#15
Joyce C Mhlanga, Alin Chirindel, Martin A Lodge, Richard L Wahl, Rathan M Subramaniam
OBJECTIVE: The aim of this study was to determine whether various fluorine-18-fluorodeoxyglucose PET/CT-derived parameters used in oncology vary significantly depending on the interpretation software systems used in clinical practice for multiple human solid tumors. PATIENTS AND METHODS: A total of 120 fluorine-18-fluorodeoxyglucose PET/CT studies carried out in patients with pancreatic, lung, colorectal, and head and neck cancers were evaluated retrospectively on two different vendor software platforms including Mirada and MIMVista...
December 8, 2017: Nuclear Medicine Communications
https://www.readbyqxmd.com/read/29227020/determination-of-herbicides-in-environmental-water-samples-by-simultaneous-in-syringe-magnetic-stirring-assisted-dispersive-liquid-liquid-microextraction-and-silylation-followed-by-gc-ms
#16
Ruth Suárez, Sabrina Clavijo, Alba González, Víctor Cerdà
An on line, fast, simple, selective and sensitive method has been developed for the determination of three herbicides belonging to the following families: triazines (atrazine), chloroacetamide (alachlor) and phenoxy (2,4-dichlorophenoxyacetic acid) in water samples. The method involves an in-syringe magnetic stirring-assisted dispersive liquid-liquid microextraction along with simultaneous silylation prior to their determination by gas chromatography with mass spectrometry. Extraction, derivatization and preconcentration have been simultaneously performed using acetone as dispersive solvent, N-methyl-N-tert-butyldimethylsilyltrifluoroacetamide as derivatization agent and trichloroethylene as extraction solvent...
December 11, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/29227018/a-combination-of-dispersive-liquid-liquid-microextraction-and-surface-plasmon-resonance-sensing-of-gold-nanoparticles-for-the-determination-of-ziram-pesticide
#17
Fatemeh Hashemi, Saadat Rastegarzadeh, Nahid Pourreza
A reliable, fast and highly sensitive analytical method utilizing dispersive liquid-liquid microextraction and gold nanoparticles probes was developed for the determination of ziram. The method is based on the in situ formation of gold nanoparticles in carbon tetrachloride as organic phase. It was found that the trace levels of ziram influenced on the formation of gold nanoparticles, leading to an absorbance change of sedimented phase. This results of the colorimetric determination of ziram were in the concentration range of 0...
December 11, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/29227014/comparison-of-weighted-and-composite-scores-for-pre-clinical-dental-learners
#18
H M Nassar, Y S Park, A Tekian
PURPOSE: The weighted mean (WM) method for combining scores of assessment components can provide outcomes with biased reliability and score precision. The objective of this study was to compare the traditional WM with another method utilising the composite score (CS) principle for combining scores of a final summative assessment exercise for a pre-clinical dental course. METHODS: Data were collected from the final examination of third-year Preclinical Operative and Esthetic Dentistry course consisting of 3 parts: practical competency, objective structured practical examination and a written examination with weights of 33%, 17% and 50%, respectively...
December 11, 2017: European Journal of Dental Education: Official Journal of the Association for Dental Education in Europe
https://www.readbyqxmd.com/read/29224926/fully-automated-detection-and-quantification-of-macular-fluid-in-oct-using-deep-learning
#19
Thomas Schlegl, Sebastian M Waldstein, Hrvoje Bogunovic, Franz Endstraßer, Amir Sadeghipour, Ana-Maria Philip, Dominika Podkowinski, Bianca S Gerendas, Georg Langs, Ursula Schmidt-Erfurth
PURPOSE: Development and validation of a fully automated method to detect and quantify macular fluid in conventional OCT images. DESIGN: Development of a diagnostic modality. PARTICIPANTS: The clinical dataset for fluid detection consisted of 1200 OCT volumes of patients with neovascular age-related macular degeneration (AMD, n = 400), diabetic macular edema (DME, n = 400), or retinal vein occlusion (RVO, n = 400) acquired with Zeiss Cirrus (Carl Zeiss Meditec, Dublin, CA) (n = 600) or Heidelberg Spectralis (Heidelberg Engineering, Heidelberg, Germany) (n = 600) OCT devices...
December 8, 2017: Ophthalmology
https://www.readbyqxmd.com/read/29224868/technical-note-validation-of-the-bhbcheck-blood-%C3%AE-hydroxybutyrate-meter-as-a-diagnostic-tool-for-hyperketonemia-in-dairy-cows
#20
K J Sailer, R S Pralle, R C Oliveira, S J Erb, G R Oetzel, H M White
Accurate cow-side blood β-hydroxybutyrate (BHB) detection meters are valuable tools for rapid diagnosis of hyperketonemia. The main objective of this study was to compare the blood BHB measured in whole blood by the BHBCheck meter (PortaCheck, Moorestown, NJ) to a previously validated meter, Precision Xtra meter (Abbott Laboratories, Abbott Park, IL) and a colorimetric laboratory assay. Samples (n = 426) were collected from postpartum primiparous and multiparous Holstein cows (n = 79 cows) enrolled in 1 of 2 experiments (Exp) with different sampling schedules (Exp 1: n = 39 cows, 58 samples; Exp 2: n = 40 cows, 368 samples)...
December 7, 2017: Journal of Dairy Science
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