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Microrna metal

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https://www.readbyqxmd.com/read/29156406/target-catalyzed-hairpin-assembly-and-metal-organic-frameworks-mediated-nonenzymatic-co-reaction-for-multiple-signal-amplification-detection-of-mir-122-in-human-serum
#1
Yuliang Li, Chao Yu, Bo Yang, Zhirui Liu, Peiyuan Xia, Qian Wang
Herein, a new type of multifunctional iron based metal-organic frameworks (PdNPs@Fe-MOFs) has been synthesized by assembly palladium nanoparticles on the surface of Fe-MIL-88NH2 MOFs microcrystals, and first applied in electrochemical biosensor for ultrasensitive detection of microRNA-122 (miR-122, a biomarker of drug-induced liver injury). The nanohybrids have not only been utilized as ideal nanocarriers for immobilization of signal probes, but also used as redox probes and electrocatalysts. In this biosensor, two hairpin probes were designed as capture probes and signal probes, respectively...
November 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29133823/identification-of-mirna-mediated-drought-responsive-multi-tiered-regulatory-network-in-drought-tolerant-rice-nagina-22
#2
Sonia Balyan, Mukesh Kumar, Roseeta Devi Mutum, Utkarsh Raghuvanshi, Priyanka Agarwal, Saloni Mathur, Saurabh Raghuvanshi
Comparative characterization of microRNA-mediated stress regulatory networks in contrasting rice cultivars is critical to decipher plant stress response. Consequently, a multi-level comparative analysis, using sRNA sequencing, degradome analysis, enzymatic and metabolite assays and metal ion analysis, in drought tolerant and sensitive rice cultivars was conducted. The study identified a group of miRNAs "Cultivar-specific drought responsive" (CSDR)-miRNAs (osa-miR159f, osa-miR1871, osa-miR398b, osa-miR408-3p, osa-miR2878-5p, osa-miR528-5p and osa-miR397a) that were up-regulated in the flag-leaves of tolerant cultivar, Nagina 22 (N22) and Vandana, but down-regulated in the sensitive cultivar, Pusa Basmati 1 (PB1) and IR64, during drought...
November 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28961476/a-metal-organic-framework-based-pcr-free-biosensor-for-the-detection-of-gastric-cancer-associated-micrornas
#3
Gui-Hua Qiu, Wan-Zhen Lu, Pei-Pei Hu, Zhi-Hong Jiang, Li-Ping Bai, Tao-Rui Wang, Min-Min Li, Jin-Xiang Chen
We report herein five sensing platforms for the detection of five gastric cancer associated microRNAs (miRNAs). The sensing platforms are hybrids formed from a water-stable metal organic framework (MOF) {[Cu(dcbb)2(H2O)2]·10H2O}n (1, H2dcbbBr=1-(3,5-dicarboxybenzyl)-4,4'-bipyridinium bromide), respectively with five carboxyfluorescein (FAM) labeled probe single-stranded DNA (probe ss-DNA, denoted as P-DNA). Within the hybrid, MOF 1 tightly interacts with the P-DNA through electrostatic and/or π-stacking interactions and results in fluorescence quenching of FAM via a photo-induced electron transfer (PET) process...
September 11, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28884170/sers-active-metal-dielectric-nanostructures-integrated-in-microfluidic-devices-for-label-free-quantitative-detection-of-mirna
#4
Chiara Novara, Alessandro Chiadò, Niccolò Paccotti, Silvia Catuogno, Carla Lucia Esposito, Gerolama Condorelli, Vittorio De Franciscis, Francesco Geobaldo, Paola Rivolo, Fabrizio Giorgis
In this work, SERS-based microfluidic PDMS chips integrating silver-coated porous silicon membranes were used for the detection and quantitation of microRNAs (miRNAs), which consist of short regulatory non-coding RNA sequences typically over- or under-expressed in connection with several diseases such as oncogenesis. In detail, metal-dielectric nanostructures which provide noticeable Raman enhancements were functionalized according to a biological protocol, adapted and optimized from an enzyme-linked immunosorbent assay (ELISA), for the detection of miR-222...
September 8, 2017: Faraday Discussions
https://www.readbyqxmd.com/read/28804566/microrna-93-inhibits-apoptosis-and-promotes-proliferation-invasion-and-migration-of-renal-cell-carcinoma-achn-cells-via-the-tgf-%C3%AE-smad-signaling-pathway-by-targeting-runx3
#5
Li-Jie Liu, Jian-Jun Yu, Xiao-Lin Xu
We investigated the ability of microRNA-93 (miR-93) to influence proliferation, invasion, migration, and apoptosisofrenal cell carcinoma (RCC) cells via transforming growth factor-β/solvated metal atom dispersed (TGF-β/Smad) signaling by targeting runt-related transcription factor 3 (RUNX3). RCC tissues with corresponding adjacent normal tissues were collected from 249 RCC patients. And normal renal tissues were collected from patients without RCC who received nephrectomy. The RCC cell line ACHN was treated with miR-93 mimic, mimic-negative control (NC), miR-93 inhibitor, inhibitor-NC, and miR-93 inhibitor + small interfering RNA (siRNA) against RUNX3 (si-RUNX3)...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28749132/universal-ratiometric-photoelectrochemical-bioassay-with-target-nucleotide-transduction-amplification-and-electron-transfer-tunneling-distance-regulation-strategies-for-ultrasensitive-determination-of-microrna-in-cells
#6
Ying-Ning Zheng, Wen-Bin Liang, Cheng-Yi Xiong, Ying Zhuo, Ya-Qin Chai, Ruo Yuan
A universal ratiometric photoelectrochemical (PEC) bioassay, which could be readily expanded for ultrasensitive determination of various targets in complex biological matrixes, was established by coupling a target-nucleotide transduction-amplification with DNA nanomachine mediated electron-transfer tunneling distance regulation strategies. With the help of target-nucleotide transduction-amplification strategy, the one input target signal could be transducted to corresponding multiple output DNA signals by nucleotide specific recognition technology, simultaneously leading to an efficient signal amplification for target...
September 5, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28679150/microarray-based-bioinformatics-analysis-of-the-combined-effects-of-sinps-and-pbac-on-cardiovascular-system-in-zebrafish
#7
Hejing Hu, Yannan Zhang, Yanfeng Shi, Lin Feng, Junchao Duan, Zhiwei Sun
With rapid development of nanotechnology and growing environmental pollution, the combined toxic effects of SiNPs and pollutants of heavy metals like lead have received global attentions. The aim of this study was to explore the cardiovascular effects of the co-exposure of SiNPs and lead acetate (PbAc) in zebrafish using microarray and bioinformatics analysis. Although there was no other obvious cardiovascular malformation except bleeding phenotype, bradycardia, angiogenesis inhibition and declined cardiac output in zebrafish co-exposed of SiNPs and PbAc at NOAEL level, significant changes were observed in mRNA and microRNA (miRNA) expression patterns...
October 2017: Chemosphere
https://www.readbyqxmd.com/read/28657742/microrna268-overexpression-affects-rice-seedling-growth-under-cadmium-stress
#8
Yanfei Ding, Yi Wang, Zhihua Jiang, Feijuan Wang, Qiong Jiang, Junwei Sun, Zhixiang Chen, Cheng Zhu
MicroRNAs (miRNAs) are 21-24-nucleotide-long RNAs that function as ubiquitous post-transcriptional regulators of gene expression in plants and animals. Increasing evidence points to the important role of miRNAs in plant responses to abiotic and biotic stresses. Cadmium (Cd) is a nonessential heavy metal highly toxic to plants. Although many genes encoding metal transporters have been characterized, the mechanisms for the regulation of the expression of the heavy-metal transporter genes are largely unknown. In this study, we found that the expression of miR268 in rice was significantly induced under Cd stress...
July 26, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28562070/the-role-of-micrornas-in-environmental-risk-factors-noise-induced-hearing-loss-and-mental-stress
#9
Verónica Miguel, Julia Yue Cui, Lidia Daimiel, Cristina Espinosa-Díez, Carlos Fernández-Hernando, Terrance J Kavanagh, Santiago Lamas
SIGNIFICANCE: MicroRNAs (miRNAs) are important regulators of gene expression and define part of the epigenetic signature. Their influence on every realm of biomedicine is established and progressively increasing. The impact of environment on human health is enormous. Among environmental risk factors impinging on quality of life are those of chemical nature (toxic chemicals, heavy metals, pollutants, and pesticides) as well as those related to everyday life such as exposure to noise or mental and psychosocial stress...
June 30, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/28502794/mir-125a-5p-modulates-phenotypic-switch-of-vascular-smooth-muscle-cells-by-targeting-ets-1
#10
C Gareri, C Iaconetti, S Sorrentino, C Covello, S De Rosa, C Indolfi
MicroRNAs are key regulators of vascular smooth muscle cells (VSMCs) phenotypic switch, one of the main events responsible for bare metal in-stent restenosis after percutaneous coronary intervention. miR-125a-5p is an important modulator of differentiation, proliferation, and migration in different cell types; however, its role in VSMCs is still unknown. The aim of this study was to evaluate the role of miR-125a-5p in VSMCs phenotypic switch. Our results suggest that miR-125a-5p is highly expressed in VSMCs, but it is down-regulated after vascular injury in vivo...
June 16, 2017: Journal of Molecular Biology
https://www.readbyqxmd.com/read/28501216/intracellular-dna-and-microrna-sensing-based-on-metal-organic-framework-nanosheets-with-enzyme-free-signal-amplification
#11
Wen-Jun Song
A new fluorescent sensing platform based on ultrathin metal-organic framework (MOF) nanosheets (MnDMS) was prepared from the flexible ligand 2,2-dimethylsuccinate and Mn ions. The MnDMS nanoparticles can be obtained by simply ultrasonication of the MnDMS crystal, and then can be exfoliated into nanosheets by Li-intercalation method. The MnDMS nanosheets can be easily assembled with biological probes, leading to efficient fluorescence quenching of the fluorophore tagged ssDNA and microRNA (miRNA). By using a hybridization chain reaction (HCR) strategy, the fluorescence signal can be obviously amplified...
August 1, 2017: Talanta
https://www.readbyqxmd.com/read/28458183/metal-enhanced-fluorescence-visual-bimodal-platform-for-multiplexed-ultrasensitive-detection-of-microrna-with-reusable-paper-analytical-devices
#12
Linlin Liang, Feifei Lan, Xuemei Yin, Shenguang Ge, Jinghua Yu, Mei Yan
Convenient biosensor for simultaneous multi-analyte detection was increasingly required in biological analysis. A novel flower-like silver (FLS)-enhanced fluorescence/visual bimodal platform for the ultrasensitive detection of multiple miRNAs was successfully constructed for the first time based on the principle of multi-channel microfluidic paper-based analytical devices (µPADs). Fluorophore-functionalized DNA1 (DNA1-N-CDs) was combined with FLS, which was hybridized with quencher-carrying strand (DNA2-CeO2) to form FLS-enhanced fluorescence biosensor...
September 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28400205/titanium-dioxide-aggregating-nanoparticles-induce-autophagy-and-under-expression-of-microrna-21-and-30a-in-a549-cell-line-a-comparative-study-with-cobalt-ii-iii-oxide-nanoparticles
#13
Rossella Alinovi, Matteo Goldoni, Silvana Pinelli, Francesca Ravanetti, Maricla Galetti, Giorgio Pelosi, Giuseppe De Palma, Pietro Apostoli, Antonio Cacchioli, Antonio Mutti, Paola Mozzoni
The toxicity of TiO2 nanoparticles (NPs) is controversial, while it is widely accepted for Co3O4 NPs. We present a comparative study concerning the uptake of these NPs and their effect on cytoplasmic organelles and autophagy in a human lung carcinoma cell line (A549), including assays on the expression of autophagy-related microRNAs. The NP accumulation caused a fast dose- and time-dependent change of flow cytometry physical parameters particularly after TiO2 NP exposure. The intracellular levels of metals confirmed it, but the Co concentration was ten times higher than that of Ti...
April 8, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28397497/semipermeable-functional-dna-encapsulated-nanocapsules-as-protective-bioreactors-for-biosensing-in-living-cells
#14
Cheng-Yi Hong, Shu-Xian Wu, Shi-Hua Li, Hong Liang, Shan Chen, Juan Li, Huang-Hao Yang, Weihong Tan
The development of functional DNA-based nanosensors in living cells has experienced some design challenges, including, for example, poor cellular uptake, rapid nuclease degradation, and high false positives. Herein, we designed selectively permeable poly(methacrylic acid) (PMA) nanocapsules to encapsulate functional DNAs for metal ions and small-molecules sensing in living cells. Since functional DNAs are concentrated in the nanocapsules, an increasing reaction rate is obtained in vitro. During endocytosis, polymeric capsules simultaneously improve cellular uptake of functional DNAs and preserve their structural integrity inside the confined capsule space...
April 25, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28317945/identification-and-comparative-profiling-of-mirnas-in-herbaceous-peony-paeonia-lactiflora-pall-with-red-yellow-bicoloured-flowers
#15
Daqiu Zhao, Mengran Wei, Min Shi, Zhaojun Hao, Jun Tao
Herbaceous peony (Paeonia lactiflora Pall.) is popular worldwide because of its gorgeous flower colour, and the yellow flower is the rarest. However, its mechanism of yellow formation is still unexplored from the post-translational level. In this study, the anatomy of the petal, cell sap pH and metal elements were investigated in bicoloured flower cultivar 'Jinhui' with red outer-petal and yellow inner-petal, and the yellow formation was influenced by the anatomy of petal, while not by the cell sap pH and metal elements...
March 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28249814/micrornas-impaired-vasculogenesis-in-metal-induced-teratogenicity
#16
Selvaraj Vimalraj, Venil N Sumantran, Suvro Chatterjee
Certain metals have been known for their toxic effects on embryos and fetal development. The vasculature in early pregnancy is extremely dynamic and plays an important role in organogenesis. Nascent blood vessels in early embryonic life are considered to be a primary and delicate target for many teratogens since the nascent blood islands follow a tightly controlled program to form vascular plexus around and inside the embryo for resourcing optimal ingredients for its development. The state of the distribution of toxic metals, their transport mechanisms and the molecular events by which they notch extra-embryonic and embryonic vasculatures are illustrated...
February 27, 2017: Reproductive Toxicology
https://www.readbyqxmd.com/read/28229383/mirna-based-heavy-metal-homeostasis-and-plant-growth
#17
REVIEW
Ali Noman, Muhammad Aqeel
Plants have been naturally gifted with mechanisms to adjust under very high or low nutrient concentrations. Heavy metal toxicity is considered as a major growth and yield-limiting factor for plants. This stress includes essential as well as non-essential metals. MicroRNAs (miRNAs) are known for mediating post-transcriptional regulation by cleaving transcripts or translational inhibition. It is commonly agreed that an extensive understanding of plant miRNAs will significantly help in the induction of tolerance against environmental stresses...
April 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28181801/optokinetically-encoded-nanoprobe-based-multiplexing-strategy-for-microrna-profiling
#18
Sungi Kim, Jeong-Eun Park, Woosung Hwang, Jinyoung Seo, Young-Kwang Lee, Jae-Ho Hwang, Jwa-Min Nam
Multiplexed real-time analysis on multiple interacting molecules and particles is needed to obtain information on binding patterns between multiple ligands and receptors, specificity of bond formations, and interacting pairs in a complex medium, often found in chemical and biological systems, and difference in binding affinity and kinetics for different binding pairs in one solution. In particular, multiplexed profiling of microRNA (miRNA) in a reliable, quantitative manner is of great demand for the use of miRNA in cell biology, biosensing, and clinical diagnostic applications, and accurate diagnosis of cancers with miRNA is not possible without detecting multiple miRNA sequences in a highly specific manner...
February 27, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28134381/dna-nanomachine-based-regenerated-sensing-platform-a-novel-electrochemiluminescence-resonance-energy-transfer-strategy-for-ultra-high-sensitive-detection-of-microrna-from-cancer-cells
#19
Pu Zhang, Zhaoyang Li, Haijun Wang, Ying Zhuo, Ruo Yuan, Yaqin Chai
The construction of DNA nanomachines holds great significance in the development of DNA nanostructures; however, the real application of nanomachines is still in its early stage. Moreover, one-step regenerated sensing platforms for the detection of biomarkers in the current research remain a practical challenge. Herein, a novel electrochemiluminescence resonance energy transfer (ERET) strategy between Alexa Flour 488 (AF 488), which is a type of small molecule dye, as the donor and CdSe@ZnS quantum dots (QDs) as the acceptor, which easily enter the cells, has been reported and was applied for the construction of a DNA nanomachine-based regenerated biosensor for the ultra-high sensitive determination of cancer cells without any enzyme...
January 30, 2017: Nanoscale
https://www.readbyqxmd.com/read/28103776/small-and-hungry-micrornas-in-micronutrient-homeostasis-of-plants
#20
Prashanti Patel, Karuna Yadav, Thumballi R Ganapathi
MicroRNAs are emerging players in plant development and response to stresses, both biotic and abiotic such as micronutrient deficiency. These small RNAs regulate cognate downstream targets either by transcript cleavage or translational inhibition. Micronutrient deficiencies lead to poor quality and yield of crops and impaired human health. Over the years several microRNAs have been identified which regulate expression of genes controlling uptake, mobilization and homeostasis of macronutrients such as nitrogen, phosphorus and sulfur to ensure sufficiency without toxicity...
2017: MicroRNA
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