Read by QxMD icon Read

Microrna copper

Anirban Kundu, Sujay Paul, Avishek Dey, Amita Pal
MicroRNAs (miRNAs) are 20-24 nucleotides long non-coding RNAs known to play important regulatory roles during biotic and abiotic stresses by controlling gene expression. Blackgram (Vigna mungo), an economically important grain legume is highly susceptible to pathogenic begomovirus Mungbean Yellow Mosaic India Virus (MYMIV) and resulting in high yield loss. In this study two different leaf-small-RNA libraries were prepared from the pooled RNA at three different time points of resistant V. mungo inbred line VM84 inoculated either with viruliferous or non-viruliferous whiteflies carrying MYMIV and performed high-throughput Illumina sequencing...
April 2017: Plant Science: An International Journal of Experimental Plant Biology
Prashanti Patel, Karuna Yadav, Thumballi Ramabhatta Ganapathi
MicroRNAs are emerging players in plant development and response to stresses, both biotic and abiotic such as micronutrient deficiency. These small RNAs regulate cognate downstream targets either by transcript cleavage or translational inhibition. Micronutrient deficiencies lead to poor quality and yield of crops and impaired human health. Over the years several microRNAs have been identified which regulate expression of genes controlling uptake, mobilization and homeostasis of macronutrients such as nitrogen, phosphorus and sulfur to ensure sufficiency without toxicity...
January 17, 2017: MicroRNA
Mariya Ostroukhova, Zhanneta Zalutskaya, Elena Ermilova
A feature of the mitochondrial electron transport chain in plants, some protists and many fungi is the presence of two terminal oxidases, the energy-conserving cytochrome oxidase and another termed alternative oxidase (AOX). AOX branches from the main respiratory chain, directly coupling the oxidation of ubiquinol with reduction of oxygen to water. The AOX genes can be divided into two discrete subfamilies, AOX1 and AOX2. Although AOX has been proposed to play essential roles in stress tolerance of organisms, the role of subfamily AOX2 is largely unknown...
December 13, 2016: European Journal of Protistology
Andreas Keller, Stephanie Kreis, Petra Leidinger, Frank Maixner, Nicole Ludwig, Christina Backes, Valentina Galata, Gea Guerriero, Tobias Fehlmann, Andre Franke, Benjamin Meder, Albert Zink, Eckart Meese
The analysis of nucleic acids in ancient samples is largely limited to DNA. Small non-coding RNAs (microRNAs) are known to be evolutionary conserved and stable. To gain knowledge on miRNAs measured from ancient samples we profiled microRNAs in cryoconserved mummies. First, we established the approach on a World War One warrior, the "Kaiserjäger", which has been preserved for almost one century. Then, we profiled seven ancient tissue specimens including skeletal muscle, stomach mucosa, stomach content and two corpus organ tissues of the 5,300 year-old copper age mummy Iceman and compared these profiles to the presence of organ-specific miRNAs in modern tissues...
December 26, 2016: Molecular Biology and Evolution
Y Shen, Y H Liu, X J Zhang, Q Sha, Z D Chen
Peanut is one of the most important oilseed crops in the world that provides a significant amount of lipids and protein for many people. The gynophore plays an important role in gynophore development after fertilization of the peanut ovule. MicroRNAs (miRNAs) play an important role in numerous developmental and physiological plant processes. Therefore, it is essential to analyze the miRNA sequences at different gynophore stages to explore and validate gene function. Multiple small RNAs were sequenced and collected from gynophore stages A1, A2, and A3 (5, 10, and 20 days of development, respectively) for further prediction...
October 24, 2016: Genetics and Molecular Research: GMR
Marinus Pilon
I. II. III. IV. V. VI. VII. VIII. References SUMMARY: Copper (Cu) microRNAs are upregulated by Cu deficiency and mediate the post-transcriptional downregulation of transcripts that encode Cu proteins, suggesting a role directly related to Cu. However, expression and phenotypic analyses of copper microRNA mutants and over-expressors have suggested roles mainly in tolerance to abiotic stresses. To reconcile available data, a model is proposed which emphasizes the mobile nature of copper microRNA molecules in the regulation of Cu homeostasis...
October 21, 2016: New Phytologist
Rachel A P Valenzuela, Kazumitsu Onizuka, Alexi A Ball-Jones, Tiannan Hu, Scott R Suter, Peter A Beal
Short interfering RNA (siRNA)-triggered gene knockdown through the RNA interference (RNAi) pathway is widely used to study gene function, and siRNA-based therapeutics are in development. However, as the guide strand of an siRNA can function like a natural microRNA (miRNA), siRNAs often repress hundreds of off-target transcripts with complementarity only to the seed region (nucleotides 2-8) of the guide strand. Here, we describe novel guide strand 3'-end modifications derived from 1-ethynylribose (1-ER) and copper-catalyzed azide-alkyne cycloaddition reactions and evaluate their impact on target versus miRNA-like off-target knockdown...
December 14, 2016: Chembiochem: a European Journal of Chemical Biology
Stavroula Tsitkanou, Paul A Della Gatta, Aaron P Russell
Amyotrophic lateral sclerosis (ALS), also known as motor neuron disease (MND), is a fatal motor neuron disorder. It results in progressive degeneration and death of upper and lower motor neurons, protein aggregation, severe muscle atrophy and respiratory insufficiency. Median survival with ALS is between 2 and 5 years from the onset of symptoms. ALS manifests as either familial ALS (FALS) (~10% of cases) or sporadic ALS (SALS), (~90% of cases). Mutations in the copper/zinc (CuZn) superoxide dismutase (SOD1) gene account for ~20% of FALS cases and the mutant SOD1 mouse model has been used extensively to help understand the ALS pathology...
2016: Frontiers in Physiology
Kwan Woo Park, Bhagwan S Batule, Kyoung Suk Kang, Ki Soo Park, Hyun Gyu Park
We devised a novel method for rapid and ultrasensitive detection of target microRNA (miRNA) by employing target-assisted isothermal exponential amplification (TAIEA) combined with poly (thymine)-templated fluorescent copper nanoparticles (CuNPs) as signaling probes. The target miRNA hybridizes to the unimolecular template DNA and works as a primer for the extension reaction to form double-stranded product, which consequently generates two nicking endonuclease recognition sites. By simultaneous nicking and displacement reactions, exponential amplification generates many poly (thymine) strands as final products, which are employed for the synthesis of fluorescent CuNPs...
October 21, 2016: Nanotechnology
Bao-Zhu Chi, Ru-Ping Liang, Wei-Bin Qiu, Yan-Hong Yuan, Jian-Ding Qiu
A new strategy based on enzymatically engineered primer extension poly-thymine (EPEPT) and nanomaterials in situ generation technology is reported for direct detection of microRNA (miRNA) in a fluorescence turn-on format using the sequential and complementary reactions catalyzed by Klenow Fragment exo(-) (KFexo(-)) and terminal deoxynucleotidyl transferase (TdTase). The short miRNA can be efficiently converted into long poly-thymine (polyT) sequences, which function as template for in situ formation of fluorescence copper nanoparticles (CuNPs) as nano-dye for detecting miRNA...
January 15, 2017: Biosensors & Bioelectronics
Diego La Mendola, Chiara Giacomelli, Enrico Rizzarelli
The description of the cell life needs not only the knowledge of its genome and proteome, but also of the location of the metal ions and their different complex species in the subcellular compartments, that is of metallome. The cross-talk among these players of the omics' world secures the cellular homeostasis by means of a complex network, the alteration of which may give rise to many diseases. Copper and zinc ions levels regulate protein expression and metal-responsive transcription factors and in many pathologies metal dyshomeostasis induces to aberrant expression of different factors...
2016: Current Topics in Medicinal Chemistry
Pan Jiang, Xiaoyue Wu, Xuemin Wang, Wenbin Huang, Qing Feng
Platinum-based drugs are the firstline of treatment for non-small cell lung cancer (NSCLC), but resistance to these drugs is a major obstacle to effective chemotherapy. Our previous study revealed that the green tea polyphenol, EGCG, induced cisplatin transporter CTR1 (copper transporter 1) and enhanced cisplatin sensitivity in ovarian cancer. In this study, we found that EGCG upregulated CTR1 and increased platinum accumulation in NSCLC (A549, H460 and H1299) cells, cDDP-resistant A549 cells and a nude mouse xenograft model...
July 12, 2016: Oncotarget
Ying Yu, Guangwen Wu, Hongmei Yuan, Lili Cheng, Dongsheng Zhao, Wengong Huang, Shuquan Zhang, Liguo Zhang, Hongyu Chen, Jian Zhang, Fengzhi Guan
BACKGROUND: MicroRNAs (miRNAs) play a critical role in responses to biotic and abiotic stress and have been characterized in a large number of plant species. Although flax (Linum usitatissimum L.) is one of the most important fiber and oil crops worldwide, no reports have been published describing flax miRNAs (Lus-miRNAs) induced in response to saline, alkaline, and saline-alkaline stresses. RESULTS: In this work, combined small RNA and degradome deep sequencing was used to analyze flax libraries constructed after alkaline-salt stress (AS2), neutral salt stress (NSS), alkaline stress (AS), and the non-stressed control (CK)...
2016: BMC Plant Biology
Bruno Printz, Stanley Lutts, Jean-Francois Hausman, Kjell Sergeant
In plants, copper (Cu) acts as essential cofactor of numerous proteins. While the definitive number of these so-called cuproproteins is unknown, they perform central functions in plant cells. As micronutrient, a minimal amount of Cu is needed to ensure cellular functions. However, Cu excess may exert in contrast detrimental effects on plant primary production and even survival. Therefore it is essential for a plant to have a strictly controlled Cu homeostasis, an equilibrium that is both tissue and developmentally influenced...
2016: Frontiers in Plant Science
Yaqin Tang, Tingting Wang, Ming Chen, Xiao He, Xiaohuan Qu, Xuli Feng
The crucial role of miRNA in cell regulation and its connection with diverse human cancers as a tumor suppressor or an oncogenic fragment poses great demand for an accurate and rapid approach for highly efficient miRNA detection and imaging in live cells. However, the ability to selectively detect and image miRNA remains a significant challenge in biomedical fields. Herein, a sealed circular probe (CP) has been prepared with copper free click ligation. The big tension force of the ring structure greatly increases the sequence recognition specificity...
November 15, 2016: Biosensors & Bioelectronics
Caroline E Gargett, Shanti Gurung
No abstract text is available yet for this article.
June 2016: Biology of Reproduction
Xurui Wang, Wei Zhu, Xiaodong Zhao, Ping Wang
The expression levels of the copper transporter P-type adenosine triphosphatase (ATP7B) are known correlate with tumor cell sensitivity to cisplatin. However, the mechanisms underlying cisplatin resistance remained poorly understood. Therefore, in the present study, we treated Hep-2 cells and in-house-developed vincristine-resistant Hep-2v cells with 50, 100, or 200 µM cisplatin and assessed cell viability after 24 or 48 h. Hep-2v cells were shown to be resistant to 50-200 µM cisplatin. Furthermore, using immunofluorescence staining and western blot analysis, we noted that ATP7B, but not copper-transporting ATPase 1 (ATP7A), expression was significantly increased in Hep-2v cells, and this increase was maintained at a higher level compared with Hep-2 cells...
June 2016: International Journal of Molecular Medicine
K Dirksen, T Verzijl, T S G A M van den Ingh, J C M Vernooij, L J W van der Laan, I A Burgener, B Spee, H Fieten
Common parenchymal liver diseases in dogs include reactive hepatopathies and primary hepatitis (acute or chronic). In chronic hepatitis, there is usually a long subclinical phase. Specific clinical signs become overt only when liver damage is severe and in this phase, treatment is usually less effective. Limited data are available regarding the sensitivity of liver enzyme activity or biomarkers for early detection of subclinical hepatitis. Hepatocyte-derived microRNAs (HDmiRs) were recently identified as promising biomarkers for hepatocellular injury in multiple species...
May 2016: Veterinary Journal
Guadalupe Aguirre, Marinus Pilon
Copper is required for photosynthesis in chloroplasts of plants because it is a cofactor of plastocyanin, an essential electron carrier in the thylakoid lumen. Other chloroplast copper proteins are copper/zinc superoxide dismutase and polyphenol oxidase, but these proteins seem to be dispensable under conditions of low copper supply when transcripts for these proteins undergo microRNA-mediated down regulation. Two ATP-driven copper transporters function in tandem to deliver copper to chloroplast compartments...
2015: Frontiers in Plant Science
Jelena Gacic, Emina Vorkapic, Renate Slind Olsen, Daniel Söderberg, Therese Gustafsson, Robert Geffers, Karin Skoglund, Andreas Matussek, Dick Wågsäter
BACKGROUND: Imatinib mesylate (Glivec®, formerly STI-571) is a selective tyrosine kinase inhibitor used for the treatment of chronic myeloid leukemia and gastrointestinal stromal tumors. However, there are reports suggesting that imatinib could be atheroprotective by lowering plasma low-density lipoprotein (LDL). AIM: To investigate the potential inhibitory effect of imatinib on cholesterol uptake in human macrophages as well as its effect on matrix metalloproteinase (MMP) activity...
February 2016: Pharmacological Reports: PR
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"