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Lipidomics and cancer

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https://www.readbyqxmd.com/read/29759594/liquid-chromatography-mass-spectrometry-based-metabolomics-and-lipidomics-reveal-toxicological-mechanisms-of-bisphenol-f-in-breast-cancer-xenografts
#1
Chao Zhao, Peisi Xie, Hailin Wang, Zongwei Cai
Bisphenol F (BPF) is a major alternative to bisphenol (BPA) and has been widely used. Although BPA exposure is known to generate various toxic effects, toxicity of BPF remains under-explored. A comprehensive method involving mass spectrometry (MS)-based global lipidomics and metabolomics, and matrix-assisted laser desorption/ionization-mass spectrometry (MALDI)- MS imaging (MSI) was used to study toxic effects of BPF and the underlying mechanisms on tumor metastasis-related tissues (liver and kidney) in breast cancer xenografts...
May 5, 2018: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/29748690/untargeted-lipidomic-analysis-of-primary-human-epidermal-melanocytes-acutely-and-chronically-exposed-to-uv-radiation
#2
Núria Dalmau, Nathalie Andrieu-Abadie, Romà Tauler, Carmen Bedia
Ultraviolet (UV) radiation present in sunlight has been related to harmful effects on skin such as premature aging and skin cancer. In order to study the effects of UV radiation on skin, many investigations have been carried out at transcriptomic and proteomic levels. However, studies on the effects of UV radiation on lipid composition are scarce. In this work, primary cultures of melanocytes were exposed to UV radiation in a similar UVA/UVB ratio to that found in solar light. The UV exposure was carried out twice a week and different endpoints were investigated at 0...
May 11, 2018: Molecular omics
https://www.readbyqxmd.com/read/29717038/lipidomic-profiling-reveals-soluble-epoxide-hydrolase-as-a-therapeutic-target-of-obesity-induced-colonic-inflammation
#3
Weicang Wang, Jun Yang, Jianan Zhang, Yuxin Wang, Sung Hee Hwang, Weipeng Qi, Debin Wan, Daeyoung Kim, Jia Sun, Katherine Z Sanidad, Haixia Yang, Yeonhwa Park, Jun-Yan Liu, Xinfeng Zhao, Xiaohui Zheng, Zhenhua Liu, Bruce D Hammock, Guodong Zhang
Obesity is associated with enhanced colonic inflammation, which is a major risk factor for colorectal cancer. Considering the obesity epidemic in Western countries, it is important to identify novel therapeutic targets for obesity-induced colonic inflammation, to develop targeted strategies for prevention. Eicosanoids are endogenous lipid signaling molecules involved in regulating inflammation and immune responses. Using an LC-MS/MS-based lipidomics approach, we find that obesity-induced colonic inflammation is associated with increased expression of soluble epoxide hydrolase (sEH) and its eicosanoid metabolites, termed fatty acid diols, in colon tissue...
May 1, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29670294/the-surgical-intelligent-knife-distinguishes-normal-borderline-and-malignant-gynaecological-tissues-using-rapid-evaporative-ionisation-mass-spectrometry-reims
#4
David L Phelps, Júlia Balog, Louise F Gildea, Zsolt Bodai, Adele Savage, Mona A El-Bahrawy, Abigail Vm Speller, Francesca Rosini, Hiromi Kudo, James S McKenzie, Robert Brown, Zoltán Takáts, Sadaf Ghaem-Maghami
BACKGROUND: Survival from ovarian cancer (OC) is improved with surgery, but surgery can be complex and tumour identification, especially for borderline ovarian tumours (BOT), is challenging. The Rapid Evaporative Ionisation Mass Spectrometric (REIMS) technique reports tissue histology in real-time by analysing aerosolised tissue during electrosurgical dissection. METHODS: Aerosol produced during diathermy of tissues was sampled with the REIMS interface. Histological diagnosis and mass spectra featuring complex lipid species populated a reference database on which principal component, linear discriminant and leave-one-patient-out cross-validation analyses were performed...
April 19, 2018: British Journal of Cancer
https://www.readbyqxmd.com/read/29608279/in-vitro-liquid-extraction-surface-analysis-mass-spectrometry-ivlesa-ms-for-direct-metabolic-analysis-of-adherent-cells-in-culture
#5
Sankha S Basu, Elizabeth C Randall, Michael S Regan, Begoña G C Lopez, Amanda R Clark, Nicholas D Schmitt, Jeffrey N Agar, Deborah A Dillon, Nathalie Y R Agar
Conventional metabolomic methods include extensive sample preparation steps and long analytical run times, increasing the likelihood of processing artifacts and limiting high throughput applications. We present here in vitro liquid extraction surface analysis mass spectrometry (ivLESA-MS), a variation on LESA-MS, performed directly on adherent cells grown in 96-well cell culture plates. To accomplish this, culture medium was aspirated immediately prior to analysis, and metabolites were extracted using LESA from the cell monolayer surface, followed by nano-electrospray ionization and MS analysis in negative ion mode...
April 2, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29605789/the-role-of-the-lipidome-in-obesity-mediated-colon-cancer-risk
#6
REVIEW
Raghav Jain, C Austin Pickens, Jenifer I Fenton
Obesity is a state of chronic inflammation influenced by lipids such as fatty acids and their secondary oxygenated metabolites deemed oxylipids. Many such lipid mediators serve as potent signaling molecules of inflammation, which can further alter lipid metabolism and lead to carcinogenesis. For example, sphingosine-1-phosphate activates cyclooxygenase-2 in endothelial cells resulting in the conversion of arachidonic acid (AA) to prostaglandin E2 (PGE2 ). PGE2 promotes colon cancer cell growth. In contrast, the less studied path of AA oxygenation via cytochrome p450 enzymes produces epoxyeicosatetraenoic acids (EETs), whose anti-inflammatory properties cause shrinking of enlarged adipocytes, a characteristic of obesity, through the liberation of fatty acids...
March 2, 2018: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/29596410/identifying-off-target-effects-of-etomoxir-reveals-that-carnitine-palmitoyltransferase-i-is-essential-for-cancer-cell-proliferation-independent-of-%C3%AE-oxidation
#7
Cong-Hui Yao, Gao-Yuan Liu, Rencheng Wang, Sung Ho Moon, Richard W Gross, Gary J Patti
It has been suggested that some cancer cells rely upon fatty acid oxidation (FAO) for energy. Here we show that when FAO was reduced approximately 90% by pharmacological inhibition of carnitine palmitoyltransferase I (CPT1) with low concentrations of etomoxir, the proliferation rate of various cancer cells was unaffected. Efforts to pharmacologically inhibit FAO more than 90% revealed that high concentrations of etomoxir (200 μM) have an off-target effect of inhibiting complex I of the electron transport chain...
March 29, 2018: PLoS Biology
https://www.readbyqxmd.com/read/29568353/reprogrammed-lipid-metabolism-in-bladder-cancer-with-cisplatin-resistance
#8
Min Young Lee, Austin Yeon, Muhammad Shahid, Eunho Cho, Vikram Sairam, Robert Figlin, Khae-Hwan Kim, Jayoung Kim
Due to its tendency to recur and acquire chemoresistance quickly, bladder cancer (BC) remains to be an elusive and difficult disease. Patients with recurrent and chemoresistant BC have an extremely poor prognosis. One possible approach that may provide insightful and valuable information regarding resistance mechanisms is looking into the lipid metabolism of BC cells. Metabolism of lipids is essential for cancer cells and is associated with the regulation of a variety of key cellular processes and functions...
March 2, 2018: Oncotarget
https://www.readbyqxmd.com/read/29567647/increased-liver-tumor-formation-in-neutral-sphingomyelinase-2-deficient-mice
#9
Liansheng Zhong, Ji Na Kong, Michael B Dinkins, Silvia Leanhart, Zhihui Zhu, Stefka D Spassieva, Haiyan Qin, Hsuan-Pei Lin, Ahmed Elsherbini, Rebecca R Wang, Xue Jiang, Mariana Nikolova-Karakashian, Guanghu Wang, Erhard Bieberich
Sphingolipids are key signaling lipids in cancer. Genome-wide studies have identified neutral sphingomyelinase-2 (nSMase2), an enzyme generating ceramide from sphingomyelin (SM), as a potential repressor for heptocellular carcinoma. However, little is known about the sphingolipids regulated by nSMase2 and their roles in liver tumor development. We discovered growth of spontaneous liver tumors in 27.3% (9 of 33) of aged male nSMase2-deficient (fro/fro) mice. Lipidomics analysis showed marked increase of SM in the tumor...
March 22, 2018: Journal of Lipid Research
https://www.readbyqxmd.com/read/29530934/lipidomic-profiling-links-the-fanconi-anemia-pathway-to-glycosphingolipid-metabolism-in-head-and-neck-cancer-cells
#10
Xueheng Zhao, Marion G Brusadelli, Sharon Sauter, Melinda Butsch Kovacic, Wujuan Zhang, Lindsey E Romick-Rosendale, Paul F Lambert, Kenneth D R Setchell, Susanne I Wells
Purpose: Mutations in Fanconi anemia (FA) genes are common in sporadic squamous cell carcinoma of the head and neck (HNSCC), and we have previously demonstrated that FA pathway depletion in HNSCC cell lines stimulates invasion. The goal of our studies was to use a systems approach in order to define FA pathway-dependent lipid metabolism and to extract lipid-based signatures and effectors of invasion in FA-deficient cells. Experimental Design: We subjected FA-isogenic HNSCC keratinocyte cell lines to untargeted and targeted lipidomics analyses to discover novel biomarkers and candidate therapeutic targets in FA-deficient cells...
March 12, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/29530061/stearoyl-coa-desaturase-1-promotes-colorectal-cancer-metastasis-in-response-to-glucose-by-suppressing-pten
#11
Hui Ran, Yemin Zhu, Ruyuan Deng, Qi Zhang, Xisheng Liu, Ming Feng, Jie Zhong, Shuhai Lin, Xuemei Tong, Qing Su
BACKGROUND: Diabetic patients have a higher risk factor for colorectal cancer (CRC) metastasis. Stearoyl-CoA desaturase 1 (SCD1), the main enzyme responsible for producing monounsaturated fatty acids(MUFA) from saturated fatty acids, is frequently deregulated in both diabetes and CRC. The function and mechanism of SCD1 in metastasis of CRC and its relevance to glucose remains largely unknown. METHODS: SCD1 expression levels were analyzed in human CRC tissues and the Cancer Browser database ( https://genome-cancer...
March 12, 2018: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/29520382/unmasking-molecular-profiles-of-bladder-cancer
#12
REVIEW
Xuan-Mei Piao, Young Joon Byun, Wun-Jae Kim, Jayoung Kim
Precision medicine is designed to tailor treatments for individual patients by factoring in each person's specific biology and mechanism of disease. This paradigm shifted from a "one size fits all" approach to "personalized and precision care" requires multiple layers of molecular profiling of biomarkers for accurate diagnosis and prediction of treatment responses. Intensive studies are also being performed to understand the complex and dynamic molecular profiles of bladder cancer. These efforts involve looking bladder cancer mechanism at the multiple levels of the genome, epigenome, transcriptome, proteome, lipidome, metabolome etc...
March 2018: Investigative and Clinical Urology
https://www.readbyqxmd.com/read/29500376/mass-spectrometry-and-multivariate-analysis-to-classify-cervical-intraepithelial-neoplasia-from-blood-plasma-an-untargeted-lipidomic-study
#13
Ana C O Neves, Camilo L M Morais, Thais P P Mendes, Boniek G Vaz, Kássio M G Lima
Cervical cancer is still an important issue of public health since it is the fourth most frequent type of cancer in women worldwide. Much effort has been dedicated to combating this cancer, in particular by the early detection of cervical pre-cancerous lesions. For this purpose, this paper reports the use of mass spectrometry coupled with multivariate analysis as an untargeted lipidomic approach to classifying 76 blood plasma samples into negative for intraepithelial lesion or malignancy (NILM, n = 42) and squamous intraepithelial lesion (SIL, n = 34)...
March 2, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29492952/discrimination-of-papillary-thyroid-cancer-from-non-cancerous-thyroid-tissue-based-on-lipid-profiling-by-mass-spectrometry-imaging
#14
Anna Wojakowska, Laura M Cole, Mykola Chekan, Katarzyna Bednarczyk, Magdalena Maksymiak, Małgorzata Oczko-Wojciechowska, Barbara Jarząb, Malcolm R Clench, Joanna Polańska, Monika Pietrowska, Piotr Widlak
INTRODUCTION: The distinction of papillary thyroid carcinomas from benign thyroid lesions has important implication for clinical man-agement. Classification based on histopathological features can be supported by molecular biomarkers, including lipidomic signatures, identified with the use of high-throughput mass spectrometry techniques. Formalin fixation is a standard procedure for stabilization and preservation of tissue samples, therefore this type of samples constitute highly valuable source of clinical material for retrospective molecular studies...
2018: Endokrynologia Polska
https://www.readbyqxmd.com/read/29452772/metabolic-and-lipidomic-reprogramming-in-renal-cell-carcinoma-subtypes-reflects-regions-of-tumor-origin
#15
Elke Schaeffeler, Florian Büttner, Anna Reustle, Verena Klumpp, Stefan Winter, Steffen Rausch, Pascale Fisel, Jörg Hennenlotter, Stephan Kruck, Arnulf Stenzl, Judith Wahrheit, Denise Sonntag, Marcus Scharpf, Falko Fend, Abbas Agaimy, Arndt Hartmann, Jens Bedke, Matthias Schwab
BACKGROUND: Renal cell carcinoma (RCC) consists of prognostic distinct subtypes derived from different cells of origin (eg, clear cell RCC [ccRCC], papillary RCC [papRCC], and chromophobe RCC [chRCC]). ccRCC is characterized by lipid accumulation and metabolic alterations, whereas data on metabolic alterations in non-ccRCC are limited. OBJECTIVE: We assessed metabolic alterations and the lipid composition of RCC subtypes and ccRCC-derived metastases. Moreover, we elucidated the potential of metabolites/lipids for subtype classification and identification of therapeutic targets...
February 13, 2018: European Urology Focus
https://www.readbyqxmd.com/read/29438405/multiplatform-plasma-metabolic-and-lipid-fingerprinting-of-breast-cancer-a-pilot-control-case-study-in-colombian-hispanic-women
#16
Mónica P Cala, Julian Aldana, Jessica Medina, Julián Sánchez, José Guio, Julien Wist, Roland J W Meesters
Breast cancer (BC) is a highly heterogeneous disease associated with metabolic reprogramming. The shifts in the metabolome caused by BC still lack data from Latin populations of Hispanic origin. In this pilot study, metabolomic and lipidomic approaches were performed to establish a plasma metabolic fingerprint of Colombian Hispanic women with BC. Data from 1H-NMR, GC-MS and LC-MS were combined and compared. Statistics showed discrimination between breast cancer and healthy subjects on all analytical platforms...
2018: PloS One
https://www.readbyqxmd.com/read/29435121/lipidomics-reveals-altered-biosynthetic-pathways-of-glycerophospholipids-and-cell-signaling-as-biomarkers-of-the-polycystic-ovary-syndrome
#17
Mariona Jové, Irene Pradas, Alba Naudí, Susana Rovira-Llopis, Celia Bañuls, Milagros Rocha, Manuel Portero-Otin, Antonio Hernández-Mijares, Victor M Victor, Reinald Pamplona
Purpose: In this work, a non-targeted approach was used to unravel changes in the plasma lipidome of PCOS patients. The aim is to offer new insights in PCOS patients strictly selected in order to avoid confounding factors such as dyslipemia, obesity, altered glucose/insulin metabolism, cardiovascular disease, or cancer. Results: Multivariate statistics revealed a specific lipidomic signature for PCOS patients without associated pathologies. This signature implies changes, mainly by down-regulation, in glycerolipid, glycerophospholipid and sphingolipid metabolism suggesting an altered biosynthetic pathway of glycerophospholipids and cell signaling as second messengers in women with PCOS...
January 12, 2018: Oncotarget
https://www.readbyqxmd.com/read/29430917/untargeted-metabolomics-reveal-lipid-alterations-upon-2-deoxyglucose-treatment-in-human-hacat-keratinocytes
#18
Pierre Le Pogam, Mickael Doué, Yann Le Page, Denis Habauzit, Maxim Zhadobov, Ronan Sauleau, Yves Le Dréan, David Rondeau
The glucose analogue 2-deoxyglucose (2-DG) impedes cancer progression in animal models and is currently being assessed as an anticancer therapy, yet the mode of action of this drug of high clinical significance has not been fully delineated. In an attempt to better characterize its pharmacodynamics, an integrative UPLC-Q-Exactive-based joint metabolomic and lipidomic approach was undertaken to evaluate the metabolic perturbations induced by this drug in human HaCaT keratinocyte cells. R-XCMS data processing and subsequent multivariate pattern recognition, metabolites identification, and pathway analyses identified eight metabolites that were most significantly changed upon a 3 h 2-DG exposure...
March 2, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29416640/development-of-suspension-cell-culture-model-to-mimic-circulating-tumor-cells
#19
Ji Young Park, Ae Lee Jeong, Hyun Jeong Joo, Sora Han, So-Hyun Kim, Hye-Youn Kim, Jong-Seok Lim, Myeong-Sok Lee, Hyung-Kyoon Choi, Young Yang
Circulating tumor cells (CTCs) are essential for the establishment of distant metastasis. Numerous studies have characterized CTCs as metastatic precursors; however, the molecular nature of CTCs has not been completely revealed yet due to the low number of CTCs in the blood stream. As an alternative approach, we developed a long-term suspension cell culture model using human breast cancer cell lines to mimic CTCs. We found that more than 40 passaged suspension cells acquired the ability to enhance metastasis like cancer stem cells...
January 2, 2018: Oncotarget
https://www.readbyqxmd.com/read/29416059/layer-specific-lipid-signatures-in-the-human-subventricular-zone-demonstrated-by-imaging-mass-spectrometry
#20
Mandana Hunter, Nicholas J Demarais, Richard L M Faull, Angus C Grey, Maurice A Curtis
The subventricular zone is a key site of adult neurogenesis and is also implicated in neurodegenerative diseases and brain cancers. In the subventricular zone, cell proliferation, migration and differentiation of nascent stem cells and neuroblasts are regulated at least in part by lipids. The human subventricular zone is distinctly layered and each layer contains discrete cell types that support the processes of neuroblast migration and neurogenesis. We set out to determine the lipid signatures of each subventricular layer in the adult human brain (n = 4)...
February 7, 2018: Scientific Reports
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