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https://www.readbyqxmd.com/read/28965046/development-and-validation-of-a-sensitive-lc-ms-ms-method-for-simultaneous-determination-of-eight-tyrosine-kinase-inhibitors-and-its-application-in-mice-pharmacokinetic-studies
#1
Yu He, Lei Zhou, Song Gao, Taijun Yin, Yifan Tu, Robert Rayford, Xiaoqiang Wang, Ming Hu
The purpose of this study was to develop and validate a robust and sensitive LC-MS/MS method for simultaneous determinations of various tyrosine kinase inhibitors (TKIs) in biological samples and to apply the method to their pharmacokinetic studies. Processed samples were injected into the UHPLC system coupled to an ESI-triple quadrupole mass spectrometer. The compounds were separated on an AcQuity UHPLC BEH C18 column (50mm×2.1mm ID, 1.7μm) using a gradient elution of acetonitrile/0.1% formic acid in water...
September 11, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28920670/fabrication-of-hierarchical-porous-carbon-nanoflakes-for-high-performance-supercapacitors
#2
Yamin Yao, Yunqiang Zhang, Li Li, Shulan Wang, Shixue Dou, Xuan Liu
In the current work, the carbon nanoflakes (CNs-Fe/KOH) and porous carbon (PC-Ni/KOH) have been produced by using Fe(NO3)3/KOH and Ni(NO3)2/KOH as the cographitization/activation catalysts to treat the natural plane tree fluff, respectively. The as-prepared carbon materials show different morphologies when treated with different metal ions. Compared with PC-Ni/KOH, the CNs-Fe/KOH have both high graphitization degree (IG/ID = 1.53) and large SBET (1416 m(2)/g). In a three-electrode setup, the CNs-Fe/KOH electrode shows a high specific capacitance of 253 F/g at 10 A/g, with a capacitance retention of 92...
October 11, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28858483/monomeric-and-dimeric-oxidomolybdenum-v-and-vi-complexes-cytotoxicity-and-dna-interaction-studies-molybdenum-assisted-c%C3%A2-n-bond-cleavage-of-salophen-ligands
#3
Sudarshana Majumder, Sagarika Pasayat, Alok K Panda, Subhashree P Dash, Satabdi Roy, Ashis Biswas, Mokshada E Varma, Bimba N Joshi, Eugenio Garribba, Chahat Kausar, Samir Kumar Patra, Werner Kaminsky, Aurélien Crochet, Rupam Dinda
Four novel dimeric bis-μ-imido bridged metal-metal bonded oxidomolybdenum(V) complexes [Mo(V)2O2L'2(1-4)] (1-4) (where L'(1-4) are rearranged ligands formed in situ from H2L(1-4)) and a new mononuclear dioxidomolybdenum(VI) complex [Mo(VI)O2L(5)] (5) synthesized from salen type N2O2 ligands are reported. This rare series of imido-bridged complexes (1-4) have been furnished from rearranged H3L'(1-4) ligands, containing an aromatic diimine (o-phenylenediamine) "linker", where Mo assisted hydrolysis followed by -C═N bond cleavage of one of the arms of the ligand H2L(1-4) took place...
August 31, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28742866/contribution-of-factor-h-binding-protein-sequence-to-the-cross-reactivity-of-meningococcal-native-outer-membrane-vesicle-vaccines-with-over-expressed-fhbp-variant-group-1
#4
Arianna Marini, Omar Rossi, Maria Grazia Aruta, Francesca Micoli, Simona Rondini, Serafina Guadagnuolo, Isabel Delany, Ian R Henderson, Adam F Cunningham, Allan Saul, Calman A MacLennan, Oliver Koeberling
Factor H-binding protein (fHbp) is an important meningococcal vaccine antigen. Native outer membrane vesicles with over-expressed fHbp (NOMV OE fHbp) have been shown to induce antibodies with broader functional activity than recombinant fHbp (rfHbp). Improved understanding of this broad coverage would facilitate rational vaccine design. We performed a pair-wise analysis of 48 surface-exposed amino acids involved in interacting with factor H, among 383 fHbp variant group 1 sequences. We generated isogenic NOMV-producing meningococcal strains from an African serogroup W isolate, each over-expressing one of four fHbp variant group 1 sequences (ID 1, 5, 9, or 74), including those most common among invasive African meningococcal isolates...
2017: PloS One
https://www.readbyqxmd.com/read/28684382/a-regulatory-role-of-androgen-in-ovarian-steroidogenesis-by-rat-granulosa-cells
#5
Toru Hasegawa, Yasuhiko Kamada, Takeshi Hosoya, Shiho Fujita, Yuki Nishiyama, Nahoko Iwata, Yuji Hiramatsu, Fumio Otsuka
Excess androgen and insulin-like growth factor (IGF)-I in the ovarian follicle has been suggested to be involved in the pathophysiology of polycystic ovary syndrome (PCOS). Here we investigated the impact of androgen and IGF-I on the regulatory mechanism of ovarian steroidogenesis using rat primary granulosa cells. It was revealed that androgen treatment with dihydrotestosterone (DHT) amplified progesterone synthesis in the presence of FSH and IGF-I, whereas it had no significant effect on estrogen synthesis by rat granulosa cells...
September 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28677837/determination-of-tigecycline-in-human-plasma-by-lc-ms-ms-and-its-application-to-population-pharmacokinetics-study-in-chinese-patients-with-hospital-acquired-pneumonia
#6
Rong Shao, Xingang Li, Yangmin Hu, Jinliang Chen, Honggang Lou, Haibin Dai
A selective, sensitive and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the determination of tigecycline (TGC) in human plasma, using tigecycline-d9 as an internal standard (IS). Analytical samples were prepared using a protein precipitation method coupled with a concentration process. The analyte and IS were separated on a reversed-phase Waters Acquity UPLC(®) BEH-C18 column (2.1 × 50 mm i.d., 1.7 μm) with a flow rate of 0.25 mL/min. The mobile phase consisted of water, containing 0...
July 5, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28656211/notch-signaling-pathway-promotes-osteogenic-differentiation-of-mesenchymal-stem-cells-by-enhancing-bmp9-smad-signaling
#7
Junjie Cao, Yalin Wei, Jing Lian, Lunyun Yang, Xiaoyan Zhang, Jiaying Xie, Qiang Liu, Jinyong Luo, Baicheng He, Min Tang
Notch is an important pathway in that it regulates cell-to-cell signal transduction, which plays an essential role in skeletal remodeling. Bone morphogenetic protein (BMP)9 has been regarded as one of the most efficient BMPs by which to induce osteogenic differentiation in mesenchymal stem cells (MSCs). Understanding the interaction between Notch and BMP9 signaling is a critical issue for optimizing the application of MSCs and BMPs in bone tissue engineering. In the present study, we investigated the role of Notch signaling in the BMP9‑induced osteogenic differentiation of MSCs...
June 22, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28627372/regulatory-role-of-melatonin-and-bmp-4-in-prolactin-production-by-rat-pituitary-lactotrope-gh3-cells
#8
Kanako Ogura-Ochi, Satoshi Fujisawa, Nahoko Iwata, Motoshi Komatsubara, Yuki Nishiyama, Naoko Tsukamoto-Yamauchi, Kenichi Inagaki, Jun Wada, Fumio Otsuka
The effects of melatonin on prolactin production and its regulatory mechanism remain uncertain. We investigated the regulatory role of melatonin in prolactin production using rat pituitary lactotrope GH3 cells by focusing on the bone morphogenetic protein (BMP) system. Melatonin receptor activation, induced by melatonin and its receptor agonist ramelteon, significantly suppressed basal and forskolin-induced prolactin secretion and prolactin mRNA expression in GH3 cells. The melatonin MT2 receptor was predominantly expressed in GH3 cells, and the inhibitory effects of melatonin on prolactin production were reversed by treatment with the receptor antagonist luzindole, suggesting functional involvement of MT2 action in the suppression of prolactin release...
June 13, 2017: Peptides
https://www.readbyqxmd.com/read/28617033/a-uplc-ms-ms-method-for-quantification-of-5%C3%AE-androst-3%C3%AE-5-6%C3%AE-triol-in-human-plasma-development-validation-and-its-application-in-clinical-pharmacokinetic-study
#9
Zhenlei Wang, Huanhuan Wang, Pei Hu, Ji Jiang, Qian Zhao
AIM: 5α-androst-3β,5,6β-triol is a novel ischemic stroke drug under clinical development. The objective of this study was to develop and validate a simple ultraperformance liquid chromatography tandem mass spectrometry method for 5α-androst-3β,5,6β-triol in human plasma and its application in clinical pharmacokinetic study. Methodology & results: After being pretreated using an automatized solid-phase extraction procedure, plasma sample was separated on a Waters(®) Acquity™ BEH C18 column (2...
June 2017: Bioanalysis
https://www.readbyqxmd.com/read/28608655/endoscopic-evaluation-of-iron-deficiency-without-anemia-does-it-have-the-same-benefit-as-in-patients-with-anemia
#10
Ana García García de Paredes, Carlos Teruel Sánchez-Vegazo, Nerea Hernanz Ruiz, Carlos Ferre Aracil, Enrique Rodríguez de Santiago, Lara Aguilera Castro, María Sierra Morales, Agustín Albillos
BACKGROUND AND AIM: The need for endoscopic investigation in patients with iron deficiency without anemia (ID) is not established. METHODS: Data from patients with ID (serum ferritin ≤20 ng/mL, normal hemoglobin) studied with upper and lower endoscopy were retrospectively analyzed. Patients evaluated for iron deficiency anemia (IDA) served as controls, matched by sex and age in a proportion 2:1. Presence, type, location and age distribution of endoscopic findings were compared...
June 13, 2017: Journal of Digestive Diseases
https://www.readbyqxmd.com/read/28587325/sildenafil-suppresses-the-proliferation-and-enhances-the-apoptosis-of-hemangioma-endothelial-cells
#11
Xiaopo He, Yiran Liu, Kai Li, Aijun Yang, Rui Wang, Shaohua Liu
Treatment of infantile hemangiomas (IH) with propranolol was first reported in 2008. Regressions of lymphatic malformations combined with pulmonary hypertension was first reported in 2012 after three children received treatment with oral sildenafil, which serves as an antagonist of phosphodiesterase isoform-5 (PDE-5). A marked expression of endothelial cells in the cytoplasm of IH tissues was obtained in our previous study. Therefore, the present study hypothesized that the antagonist of PDE-5, sildenafil, may lead to the regression of hemangiomas...
June 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28549790/the-inhibitor-of-differentiation-1-id1-enables-lung-cancer-liver-colonization-through-activation-of-an-emt-program-in-tumor-cells-and-establishment-of-the-pre-metastatic-niche
#12
Eduardo Castañón, Alex Soltermann, Inés López, Marta Román, Margarita Ecay, María Collantes, Miriam Redrado, Iosune Baraibar, José María López-Picazo, Christian Rolfo, Fernando Vidal-Vanaclocha, Luis Raez, Walter Weder, Alfonso Calvo, Ignacio Gil-Bazo
Id1 promotes carcinogenesis and metastasis, and predicts prognosis of non-small cell lung cancer (NSCLC)-adenocarcionoma patients. We hypothesized that Id1 may play a critical role in lung cancer colonization of the liver by affecting both tumor cells and the microenvironment. Depleted levels of Id1 in LLC (Lewis lung carcinoma cells, LLC shId1) significantly reduced cell proliferation and migration in vitro. Genetic loss of Id1 in the host tissue (Id1(-/-) mice) impaired liver colonization and increased survival of Id1(-/-) animals...
August 28, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28529558/sirna-mediated-knockdown-of-id1-disrupts-nanog-and-oct-4-mediated-cancer-stem-cell-likeness-and-resistance-to-chemotherapy-in-gastric-cancer-cells
#13
Linlin Li, Xiaoyong Wei, Baofeng Wu, Yuanli Xiao, Mingzhu Yin, Qiaohong Yang
DNA-binding protein inhibitor ID-1 (ID1) serves an essential role in tumor progression, and the self-renewal and pluripotency of embryonic stem cells. However, the effect of ID1 on the stemness and cancer stem cell (CSC)-like properties of gastric adenocarcinoma cells remains to be elucidated. In the present study, effective ID1 knockdown was achieved in gastric cancer (GC) cells using small interfering RNA, and the self-renewal ability and cisplatin (DDP) sensitivity of GC cells was subsequently examined. ID1 knockdown in the MKN-28 and MGC-803 cell lines was demonstrated to significantly suppress colony formation (P=0...
May 2017: Oncology Letters
https://www.readbyqxmd.com/read/28471350/antiviral-therapy-reduces-risk-of-haemorrhagic-stroke-in-patients-with-hcv-infection-a-nationwide-cohort-study
#14
Ming-Shyan Lin, Chang-Min Chung, Wey-Yil Lin, Kuo-Liang Wei, Jui Wang, Ying-Ying Lee, Jing-Hong Hu, Tao-Hsin Tung, Yu-Sheng Lin
BACKGROUND: The tendency for haemorrhagic stroke in patients with chronic hepatitis C virus (HCV) infection has emerged recently but the finding may be confounded by comorbidities. Proving the causality between HCV infection and haemorrhagic stroke is mandatory. Our study was designed to investigate the incidence of intracranial haemorrhage in HCV-infected patients with and without treatment. METHODS: In the 11-year and population-based retrospective study, we acquired data from the Taiwan National Health Insurance Research Database...
May 4, 2017: Antiviral Therapy
https://www.readbyqxmd.com/read/28428134/use-of-physiological-biomarkers-in-diagnosis-along-with-field-trials-of-different-trypanisidal-drugs-in-camels-of-cholistan-desert
#15
Aneela Zameer Durrani, Zubair Bashir, Khalid Mehmood, Muhammad Avais, Haroon Akbar, Waqas Ahmad, Muhammad Azeem
The point prevalence of trypanosomiasis with different physiological biomarkers along with evaluation of the most responsive trypanosidal drug against trypanosomiasis under field conditions was studied. For this purpose a total of 300 free range camels were selected at different grazing and watering point in Cholistan desert. The study population of camels included 150 clinically suspected camels for trypanosomiasis and 150 healthy camels with normal temperature, pulse and respiration. For therapeutic trials 36 positively diagnosed animals were randomly divided into three experimental groups for therapeutic trials...
July 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28415442/small-intestinal-submucosa-a-potential-osteoconductive-and-osteoinductive-biomaterial-for-bone-tissue-engineering
#16
Mei Li, Chi Zhang, Mengjie Cheng, Qiaoqiao Gu, Jiyuan Zhao
SIS is an acellular, naturally occurring collagenous extracellular matrix (ECM) material with various bioactive factors, which broadly applied in tissue engineering in clinic. Several studies have applied SIS in bone tissue engineering to enhance bone regeneration in animal models. However, the mechanism was rarely investigated. The aim of the current study was to investigate the osteoconductivity and osteoinductivity of SIS scaffold to bone regeneration systematically and the potential mechanism. Our results showed that SIS scaffold with excellent biocompatibility was beneficial for cell attachment, proliferation, migration and osteogenic differentiation of various cells contributing to bone repair...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28382387/high-soluble-endoglin-levels-do-not-induce-changes-in-structural-parameters-of-mouse-heart
#17
Jana Rathouska, Petra Fikrova, Alena Mrkvicova, Katerina Blazickova, Michala Varejckova, Eva Dolezelova, Ivana Nemeckova, Barbora Vitverova, Lenka Peslova, Eunate Gallardo-Vara, Miguel Pericacho, Petr Nachtigal
A soluble form of endoglin (sEng) released into the circulation was suggested to be a direct inducer of endothelial dysfunction, inflammation and contributed to the development of hypertension by interfering with TGF-β signaling in cardiovascular pathologies. In the present study, we assessed the hypothesis that high sEng level-induced hypertension via a possible sEng interference with TGF-β signaling pathways may result in inflammatory, structural or fibrotic changes in hearts of Sol-Eng+ mice (mice with high levels of soluble endoglin) fed either chow or high-fat diet...
August 2017: Heart and Vessels
https://www.readbyqxmd.com/read/28336397/soluble-endoglin-modulates-the-pro-inflammatory-mediators-nf-%C3%AE%C2%BAb-and-il-6-in-cultured-human-endothelial-cells
#18
Michala Varejckova, Eunate Gallardo-Vara, Matej Vicen, Barbora Vitverova, Petra Fikrova, Eva Dolezelova, Jana Rathouska, Alena Prasnicka, Katerina Blazickova, Stanislav Micuda, Carmelo Bernabeu, Ivana Nemeckova, Petr Nachtigal
AIMS: Endoglin is a transmembrane glycoprotein, that plays an important role in regulating endothelium. Proteolytic cleavage of membrane endoglin releases soluble endoglin (sEng), whose increased plasma levels have been detected in diseases related to the cardiovascular system. It was proposed that sEng might damage vascular endothelium, but detailed information about the potential mechanisms involved is not available. Thus, we hypothesized that sEng contributes to endothelial dysfunction, leading to a pro-inflammatory phenotype by a possible modulation of the TGF-β and/or inflammatory pathways...
April 15, 2017: Life Sciences
https://www.readbyqxmd.com/read/28288784/development-and-validation-of-a-measurement-procedure-based-on-ultra-high-performance-liquid-chromatography-tandem-mass-spectrometry-for-simultaneous-measurement-of-%C3%AE-lactam-antibiotic-concentration-in-human-plasma
#19
Raül Rigo-Bonnin, Alba Ribera, Ariadna Arbiol-Roca, Sara Cobo-Sacristán, Ariadna Padullés, Òscar Murillo, Evelyn Shaw, Rosa Granada, Xosé L Pérez-Fernández, Fe Tubau, Pedro Alía
BACKGROUND: The administration of β-lactam antibiotics in continuous infusion could let optimize the pharmacokinetic/pharmacodynamic parameters, especially in the treatment of serious bacterial infections. In this context, and also due to variability in their plasmatic concentrations, therapeutic drug monitoring (TDM) may be useful to optimize dosing and, therefore, be useful for the clinicians. MATERIAL AND METHODS: We developed and validated a measurement procedure based on ultra-high performance liquid chromatography-tandem mass spectrometry for simultaneous measurement of amoxicillin, ampicillin, cloxacillin, piperacillin, cefepime, ceftazidime, cefuroxime, aztreonam and meropenem concentrations in plasma...
March 10, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28208103/caring-for-children-with-intellectual-disabilities-part-1-experience-with-the-population-pain-related-beliefs-and-care-decisions
#20
Lara M Genik, C Meghan McMurtry, Lynn M Breau
Some children with intellectual disabilities (ID): experience pain more frequently than children without ID, express their pain differently, and are incapable of providing self-reports. No research has examined disability and pain-related beliefs of respite workers (RW) and their relations to pain assessment and management decisions for children with ID. OBJECTIVES: (1) compare disability and pain-related beliefs between RW and a sample with little experience in ID; (2) determine whether individuals' beliefs and personal characteristics are related to pain assessment and management decisions...
March 2017: Research in Developmental Disabilities
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