keyword
MENU ▼
Read by QxMD icon Read
search

ID1

keyword
https://www.readbyqxmd.com/read/28637209/purification-and-characterization-of-two-new-cell-bound-bioactive-compounds-produced-by-wildlactococcus-lactisstrain
#1
Margarete Alice Fontes Saraiva, Dag Anders Brede, Ingolf Figved Nes, Maria Cristina Baracat-Pereira, Marisa Vieira de Queiroz, Célia Alencar de Moraes
Novel compounds and innovative methods are required considering that antibiotic resistance has reached a crisis point. In the study, two cell-bound antimicrobial compounds produced by L. lactis ID1.5 were isolated and partially characterized. Following purification by cationic exchange and a solid phase C18 column, antimicrobial activity was recovered after three runs of RPC using 60% (v/v) and 100% (v/v) of 2-propanol for elution, suggesting that more than one antimicrobial compound were produced by L. lactis ID1...
June 20, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28624363/brazilian-red-propolis-effects-on-peritoneal-macrophage-activity-nitric-oxide-cell-viability-pro-inflammatory-cytokines-and-gene-expression
#2
Bruno Bueno-Silva, Dione Kawamoto, Ellen S Ando-Suguimoto, Renato C V Casarin, Severino M Alencar, Pedro L Rosalen, Marcia P A Mayer
ETHNOPHARMACOLOGICAL RELEVANCE: Propolis has been used in folk medicine since ancient times and it presented inhibitory effect on neutrophil recruitment previously. However, its effect on macrophage obtained from mice remains unclear. AIM OF THE STUDY: to demonstrate BRP effects on LPS activated peritoneal macrophage. MATERIALS AND METHODS: Peritoneal macrophages, obtained from C57BL6 mice and activated with LPS, were treated with 50 to 80µg/mL of crude extract of Brazilian red propolis (BRP) during 48hours...
June 14, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28611292/ring-finger-protein-125-as-a-potential-highly-aggressive-and-unfavorable-prognostic-biomarker-promotes-the-invasion-and-metastasis-of-human-gallbladder-cancers-via-activating-the-tgf-%C3%AE-1-smad3-id1-signaling-pathway
#3
Zhong-Yan Liu, Jin Cao, Jing-Tao Zhang, Guo-Li Xu, Xin-Ping Li, Fang-Tao Wang, Kamar Hasan Ansari, Hassan Mohamed, Yue-Zu Fan
Human gallbladder cancer (GBC) is a lethal aggressive malignant neoplasm. Identification of potential molecular biomarkers and development of targeted therapeutics for GBC patients is very necessary. In this study, we firstly investigated the correlation between ring finger protein 125 (RNF125) expression and the metastasis and prognosis of GBC, and the underlying molecular mechanism. RNF125 expression in a cohort of GBC tissues was examined; its correlation with clinicopathological and prognostic factors of GBC patients was analyzed...
May 25, 2017: Oncotarget
https://www.readbyqxmd.com/read/28596553/hematopoietic-id-deletion-triggers-endomyocardial-fibrotic-and-vascular-defects-in-the-adult-heart
#4
Corey Chang, Qingshi Zhao, J Patrick Gonzalez, Jung H Kim, Kamal Alzahrani, Dominic Del Re, Diego Fraidenraich
Inhibitor of DNA binding (Id) proteins play important roles in regulating cardiac development via paracrine signaling. Id1/Id3 knockout mice die at mid-gestation with multiple cardiac defects. Single Id knockout studies have not reported cardiomyopathies. To bypass embryonic lethality we used Tie2CRE-mediated recombination to conditionally delete Id1 against global Id3 ablation (Id cDKOs), which develops adult-onset dilated cardiomyopathy. We confirm upregulation of thrombospondin-1 (TSP1) in Id cDKO hearts...
June 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28589682/expansion-of-adipose-tissue-derived-stromal-cells-at-physiologic-hypoxia-attenuates-replicative-senescence
#5
Andrey Ratushnyy, Margarita Lobanova, Ludmila B Buravkova
Multipotent mesenchymal stromal cells are considered as a perspective tool in cell therapy and regenerative medicine. Unfortunately, autologous cell therapy does not always provide positive outcomes in elder donors, perhaps as a result of the alterations of stem cell compartments. The mechanisms of stem and progenitor cell senescence and the factors engaged are investigated intensively. In present paper, we elucidated the effects of tissue-related O2 on morphology, functions, and transcriptomic profile of adipose tissue-derived stromal cells (ASCs) in replicative senescence in vitro model...
June 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28582447/action-of-ym155-on-clear-cell-renal-cell-carcinoma-does-not-depend-on-survivin-expression-levels
#6
Mei Yi Sim, Hung Huynh, Mei Lin Go, John Shyi Peng Yuen
The dioxonapthoimidazolium YM155 is a survivin suppressant which has been investigated as an anticancer agent in clinical trials. Here, we investigated its growth inhibitory properties on a panel of immortalized and patient derived renal cell carcinoma (RCC) cell lines which were either deficient in the tumour suppressor von Hippel-Lindau (VHL) protein or possessed a functional copy. Neither the VHL status nor the survivin expression levels of these cell lines influenced their susceptibility to growth inhibition by YM155...
2017: PloS One
https://www.readbyqxmd.com/read/28576120/expression-of-genes-associated-with-bmp-signaling-pathway-in-porcine-oocytes-before-and-after-ivm-a-microarray-approach
#7
Joanna Budna, Marta Rybska, Sylwia Ciesiółka, Artur Bryja, Sylwia Borys, Wiesława Kranc, Katarzyna Wojtanowicz-Markiewicz, Michal Jeseta, Ewa Sumelka, Dorota Bukowska, Paweł Antosik, Klaus P Brüssow, Małgorzata Bruska, Michał Nowicki, Maciej Zabel, Bartosz Kempisty
BACKGROUND: The full maturational capability of mammalian oocytes is accompanied by nuclear and cytoplasmic modifications, which are associated with proliferation and differentiation of surrounding cumulus cells. These events are regulated on molecular level by the expression of target genes involved in signal transduction pathways crucial for folliculogenesis and oogenesis. Transforming growth factor beta signaling includes several molecules that are involved in the regulation of oogenesis and embryo growth, including bone morphogenetic protein (BMP)...
June 2, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28549790/the-inhibitor-of-differentiation-1-id1-enables-lung-cancer-liver-colonization-through-activation-of-an-emt-program-in-tumor-cells-and-establishment-of-the-pre-metastatic-niche
#8
Eduardo Castañón, Alex Soltermann, Inés López, Marta Román, Margarita Ecay, María Collantes, Miriam Redrado, Iosune Baraibar, José María López-Picazo, Christian Rolfo, Fernando Vidal-Vanaclocha, Luis Raez, Walter Weder, Alfonso Calvo, Ignacio Gil-Bazo
Id1 promotes carcinogenesis and metastasis, and predicts prognosis of non-small cell lung cancer (NSCLC)-adenocarcionoma patients. We hypothesized that Id1 may play a critical role in lung cancer colonization of the liver by affecting both tumor cells and the microenvironment. Depleted levels of Id1 in LLC (Lewis lung carcinoma cells, LLC shId1) significantly reduced cell proliferation and migration in vitro. Genetic loss of Id1 in the host tissue (Id1(-/-) mice) impaired liver colonization and increased survival of Id1(-/-) animals...
May 24, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28529558/sirna-mediated-knockdown-of-id1-disrupts-nanog-and-oct-4-mediated-cancer-stem-cell-likeness-and-resistance-to-chemotherapy-in-gastric-cancer-cells
#9
Linlin Li, Xiaoyong Wei, Baofeng Wu, Yuanli Xiao, Mingzhu Yin, Qiaohong Yang
DNA-binding protein inhibitor ID-1 (ID1) serves an essential role in tumor progression, and the self-renewal and pluripotency of embryonic stem cells. However, the effect of ID1 on the stemness and cancer stem cell (CSC)-like properties of gastric adenocarcinoma cells remains to be elucidated. In the present study, effective ID1 knockdown was achieved in gastric cancer (GC) cells using small interfering RNA, and the self-renewal ability and cisplatin (DDP) sensitivity of GC cells was subsequently examined. ID1 knockdown in the MKN-28 and MGC-803 cell lines was demonstrated to significantly suppress colony formation (P=0...
May 2017: Oncology Letters
https://www.readbyqxmd.com/read/28510612/suppression-of-id1-expression-in-colon-cancer-cells-increases-sensitivity-to-5-fluorouracil
#10
Tomasz Przybyła, Monika Sakowicz-Burkiewicz, Izabela Maciejewska, Hanna Bielarczyk, Tadeusz Pawełczyk
Adjuvant chemotherapy with 5-fluorouracil remains the basic treatment for patients with advanced colorectal carcinoma. The major obstacle in successful treatment is the ability of CRC cells to acquire chemoresistance. Here we examined the impact of ID1 silencing on the sensitivity of CRC cells to 5-FU. To suppress ID1 expression in HT-29 and HCT-116 cells the cells were transduced with a lentiviral vector carrying the ID1 silencing sequence. Cells with silenced ID1 showed altered expression of epithelial and mesenchymal markers and exhibited increased proliferation rate compared to the parental cells...
2017: Acta Biochimica Polonica
https://www.readbyqxmd.com/read/28470180/associations-of-the-intellectual-disability-gene-myt1l-with-helix-loop-helix-gene-expression-hippocampus-volume-and-hippocampus-activation-during-memory-retrieval
#11
Agnieszka Kepa, Lourdes Martinez Medina, Susanne Erk, Deepak P Srivastava, Alinda Fernandes, Roberto Toro, Sabine Lévi, Barbara Ruggeri, Cathy Fernandes, Franziska Degenhardt, Stephanie H Witt, Andreas Meyer-Lindenberg, Jean-Christophe Poncer, Jean-Luc Martinot, Marie-Laure Paillère Martinot, Christian P Müller, Andreas Heinz, Henrik Walter, Gunter Schumann, Sylvane Desrivières
The fundamental role of the brain-specific myelin transcription factor 1-like (MYT1L) gene in cases of intellectual disability and in the etiology of neurodevelopmental disorders is increasingly recognized. Yet, its function remains under-investigated. Here, we identify a network of helix-loop-helix (HLH) transcriptional regulators controlled by MYT1L, as indicated by our analyses in human neural stem cells and in the human brain. Using cell-based knockdown approaches and microarray analyses we found that (1) MYT1L is required for neuronal differentiation and identified ID1, a HLH inhibitor of premature neurogenesis, as a target...
May 4, 2017: Neuropsychopharmacology: Official Publication of the American College of Neuropsychopharmacology
https://www.readbyqxmd.com/read/28427194/alternative-rna-splicing-of-the-meaf6-gene-facilitates-neuroendocrine-prostate-cancer-progression
#12
Ahn R Lee, Yinan Li, Ning Xie, Martin E Gleave, Michael E Cox, Colin C Collins, Xuesen Dong
Although potent androgen receptor pathway inhibitors (ARPI) improve overall survival of metastatic prostate cancer patients, treatment-induced neuroendocrine prostate cancer (t-NEPC) as a consequence of the selection pressures of ARPI is becoming a more common clinical issue. Improved understanding of the molecular biology of t-NEPC is essential for the development of new effective management approaches for t-NEPC. In this study, we identify a splice variant of the MYST/Esa1-associated factor 6 (MEAF6) gene, MEAF6-1, that is highly expressed in both t-NEPC tumor biopsies and neuroendocrine cell lines of prostate and lung cancers...
April 25, 2017: Oncotarget
https://www.readbyqxmd.com/read/28425640/profiling-of-the-silica-induced-molecular-events-in-lung-epithelial-cells-using-the-rna-seq-approach
#13
Judy Y W Chan, Joseph C C Tsui, Patrick T W Law, Winnie K W So, Doris Y P Leung, Michael M K Sham, Stephen K W Tsui, Carmen W H Chan
Silicosis is a prolonged, irreversible and incurable occupational disease, and there is a significant number of newly diagnosed cases every year in Hong Kong. Due to the long latency of the disease, the diagnosis can be missed until detailed clinical examination at a later stage. For a better control of this deadly disease, detailing the pro-inflammatory and fibrotic events in the macrophage would be instrumental in understanding the pathogenesis of the disease and essential for the significant biomarkers discovery...
April 20, 2017: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/28386736/an-efficient-rapid-system-for-assaying-hbx-mediated-transactivation
#14
Xiaoling Zhou, Haijun Shi, Shaozhe Yang, Pingnan Sun
OBJECTIVES: To develop a rapid and accurate assay system for screening inhibitors or enhancing agents targeting the transactivation capability of hepatitis B virus X protein (HBx) that activates cellular promoters in host cells to facilitate viral replication. RESULTS: We constructed a new GFP-based reporter system which was different from a luciferase-based reporter system. Firstly, a FLAG-tagged HBx gene was inserted into an expression plasmid, resulting in plasmid pHBx...
April 6, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28270523/id1-promotes-obesity-by-suppressing-brown-adipose-thermogenesis-and-white-adipose-browning
#15
Mallikarjun Patil, Bal Krishan Sharma, Sawsan Elattar, Judith Chang, Shweta Kapil, Jinling Yuan, Ande Satyanarayana
Obesity results from increased energy intake or defects in energy expenditure. Brown adipose tissue (BAT) is specialized for energy expenditure, a process called adaptive thermogenesis. Peroxisome proliferator-activated receptor γ coactivator 1α (PGC1α) controls BAT-mediated thermogenesis by regulating the expression of Ucp1 Inhibitor of differentiation 1 (Id1) is a helix-loop-helix transcription factor that plays an important role in cell proliferation and differentiation. We demonstrate a novel function of Id1 in BAT thermogenesis and programming of beige adipocytes in white adipose tissue (WAT)...
June 2017: Diabetes
https://www.readbyqxmd.com/read/28258199/id2-collaborates-with-id3-to-suppress-invariant-nkt-and-innate-like-tumors
#16
Jia Li, Sumedha Roy, Young-Mi Kim, Shibo Li, Baojun Zhang, Cassandra Love, Anupama Reddy, Deepthi Rajagopalan, Sandeep Dave, Anna Mae Diehl, Yuan Zhuang
Inhibitor of DNA binding (Id) proteins, including Id1-4, are transcriptional regulators involved in promoting cell proliferation and survival in various cell types. Although upregulation of Id proteins is associated with a broad spectrum of tumors, recent studies have identified that Id3 plays a tumor-suppressor role in the development of Burkitt's lymphoma in humans and hepatosplenic T cell lymphomas in mice. In this article, we report rapid lymphoma development in Id2/Id3 double-knockout mice that is caused by unchecked expansion of invariant NKT (iNKT) cells or a unique subset of innate-like CD1d-independent T cells...
April 15, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28258196/downregulation-of-e-protein-activity-augments-an-ilc2-differentiation-program-in-the-thymus
#17
Hong-Cheng Wang, Liangyue Qian, Ying Zhao, Joni Mengarelli, Indra Adrianto, Courtney G Montgomery, Joseph F Urban, Kar-Ming Fung, Xiao-Hong Sun
Innate lymphoid cells (ILCs) are important regulators in various immune responses. The current paradigm states that all newly made ILCs originate from common lymphoid progenitors in the bone marrow. Id2, an inhibitor of E protein transcription factors, is indispensable for ILC differentiation. Unexpectedly, we found that ectopically expressing Id1 or deleting two E protein genes in the thymus drastically increased ILC2 counts in the thymus and other organs where ILC2 normally reside. Further evidence suggests a thymic origin of these mutant ILC2s...
April 15, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28254723/semicarbazide-induced-thyroid-disruption-in-japanese-flounder-paralichthys-olivaceus-and-its-potential-mechanisms
#18
Zonghao Yue, Miao Yu, Xiaona Zhang, Yifei Dong, Hua Tian, Wei Wang, Shaoguo Ru
Semicarbazide (SMC) is a carcinogenic and genotoxic substance that has been found in aquatic systems. SMC may also cause thyroid follicular epithelial cell injury in rats, but the thyroid-disrupting properties of SMC and its potential mechanisms remain unclear. In this study, we exposed fertilized eggs of Japanese flounder (Paralichthys olivaceus) to 1, 10, 100, and 1000μg/L SMC for 55 d to assess the impact of SMC exposure on the thyroid system. The number of larvae in each metamorphic stage was counted, the concentrations of whole-body thyroid hormones (THs) 3,5,3'-triiodothyronine (T3) and thyroxine (T4) were measured, and the transcription levels of genes involved in the hypothalamic-pituitary-thyroid (HPT) axis and gamma-aminobutyric acid (GABA) synthesis were quantified...
June 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28245840/prognostic-stratification-improvement-by-integrating-id1-id3-igj-gene-expression-signature-and-immunophenotypic-profile-in-adult-patients-with-b-all
#19
Nataly Cruz-Rodriguez, Alba L Combita, Leonardo J Enciso, Lauren F Raney, Paula L Pinzon, Olga C Lozano, Alba M Campos, Niyireth Peñaloza, Julio Solano, Maria V Herrera, Jovanny Zabaleta, Sandra Quijano
BACKGROUND: Survival of adults with B-Acute Lymphoblastic Leukemia requires accurate risk stratification of patients in order to provide the appropriate therapy. Contemporary techniques, using clinical and cytogenetic variables are incomplete for prognosis prediction. METHODS: To improve the classification of adult patients diagnosed with B-ALL into prognosis groups, two strategies were examined and combined: the expression of the ID1/ID3/IGJ gene signature by RT-PCR and the immunophenotypic profile of 19 markers proposed in the EuroFlow protocol by Flow Cytometry in bone marrow samples...
February 28, 2017: Journal of Experimental & Clinical Cancer Research: CR
https://www.readbyqxmd.com/read/28238764/cullin-e3-ligase-activity-is-required-for-myoblast-differentiation
#20
Jordan Blondelle, Paige Shapiro, Andrea A Domenighetti, Stephan Lange
The role of cullin E3-ubiquitin ligases for muscle homeostasis is best known during muscle atrophy, as the cullin-1 substrate adaptor atrogin-1 is among the most well-characterized muscle atrogins. We investigated whether cullin activity was also crucial during terminal myoblast differentiation and aggregation of acetylcholine receptors for the establishment of neuromuscular junctions in vitro. The activity of cullin E3-ligases is modulated through post-translational modification with the small ubiquitin-like modifier nedd8...
April 7, 2017: Journal of Molecular Biology
keyword
keyword
120844
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"