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Plasma microRNA

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https://www.readbyqxmd.com/read/29783093/a-direct-plasma-mirna-assay-for-early-detection-and-histological-classification-of-lung-cancer
#1
Qixin Leng, Yue Wang, Feng Jiang
Cell-free microRNAs in plasma provide circulating biomarkers for lung cancer. Most techniques for analysis of miRNAs require a large plasma volume to purify a sufficient RNA yield followed by complicated downstream processing. Small differences in the multiple procedures often cause large analytical variations and poor diagnostic values of the plasma biomarkers. Here we investigate whether directly quantifying plasma miRNAs without RNA purification could diagnose lung cancer. FirePlex assay was directly applied to 20 μl plasma of 56 lung cancer patients and 28 cancer free controls for quantifying 11 lung tumor-associated miRNAs...
May 17, 2018: Translational Oncology
https://www.readbyqxmd.com/read/29779470/ascites-derived-extracellular-micrornas-as-potential-biomarkers-for-ovarian-cancer
#2
Luděk Záveský, Eva Jandáková, Vít Weinberger, Luboš Minář, Veronika Hanzíková, Daniela Dušková, Lenka Záveská Drábková, Iveta Svobodová, Aleš Hořínek
Ovarian cancer as the most fatal gynecological malignancy is often manifested by excessive fluid accumulation known as ascites or effusion. Ascites-derived microRNAs (miRNAs) may be closely associated with ovarian cancer progression. However, our knowledge of their roles, altered expression, and clinical outcomes remained limited. In this study, large-scale expression profiling of 754 human miRNAs was performed using real-time quantitative polymerase chain reaction and 384-well TaqMan array human miRNA A and B cards to identify differentially expressed miRNAs between extracellular fraction of the ascitic fluid associated with high-grade serous ovarian carcinomas and control plasma...
January 1, 2018: Reproductive Sciences
https://www.readbyqxmd.com/read/29777724/novel-mirna-biomarkers-for-genotoxicity-screening-in-mouse
#3
Hiroyuki Oka, Koichi Masuno, Takeki Uehara, Toru Okamoto, Yoshiharu Matsuura, Toru Nakano, Shinpei Yamaguchi
The genotoxic potential of drugs is a serious problem, and its evaluation is one of the most critical processes of drug development. Although the comet assay of compound-exposed tissue is a frequently used genotoxicity test, its high false-positive rate is a major complication, and we consistently obtained false-positive results using the comet assay of mouse liver for nine hepatotoxic non-genotoxins (NGTXs). To identify novel genotoxin (GTX)-specific biomarkers, we screened the expression of 750 microRNAs (miRNAs) in the livers of mice treated with GTXs or NGTXs...
May 16, 2018: Toxicology
https://www.readbyqxmd.com/read/29775158/microrna-expression-in-tumour-tissue-and-plasma-in-patients-with-newly-diagnosed-metastatic-prostate-cancer
#4
Ahmed Hussein Zedan, Torben Frøstrup Hansen, Jannie Assenholt, Mindaugas Pleckaitis, Jonna Skov Madsen, Palle Jörn Sloth Osther
Prostate cancer is the most common cancer among men in the western world. Clinical practice is continuously challenged by the pitfalls of the available diagnostic tools. microRNAs may represent promising biomarkers in many types of human cancers, including prostate cancer. The aim of this study was to investigate microRNA expression in tumour tissue and matched plasma in a cohort of patients with primary metastatic prostate cancer. The relative expression of 12 microRNAs was assessed in diagnostic needle biopsies from the prostate and matched plasma samples in two prospective cohorts (screening cohorts) comprising 21 patients with metastatic prostate cancer and 25 control patients...
May 2018: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/29774080/microrna-193b-3p-regulates-chondrogenesis-and-chondrocyte-metabolism-by-targeting-hdac3
#5
Fangang Meng, Zhiwen Li, Zhiqi Zhang, Zibo Yang, Yan Kang, Xiaoyi Zhao, Dianbo Long, Shu Hu, Minghui Gu, Suiwen He, Peihui Wu, Zongkun Chang, Aishan He, Weiming Liao
Histone deacetylase 3 (HDAC3) plays a pivotal role in the repression of cartilage-specific gene expression in human chondrocytes. The aim of this study was to determine whether microRNA-193b-3p (miR-193b-3p) regulates the expression of HDAC3 during chondrogenesis and chondrocyte metabolism. Methods: miR-193b-3p expression was assessed in a human mesenchymal stem cell (hMSC) model of chondrogenesis, in interleukin-1β (IL-1β)-treated primary human chondrocytes (PHCs), and in non-degraded and degraded cartilage...
2018: Theranostics
https://www.readbyqxmd.com/read/29769437/exosome-delivered-micrornas-promote-ifn-%C3%AE-secretion-by-human-plasmacytoid-dcs-via-tlr7
#6
Valentina Salvi, Veronica Gianello, Sara Busatto, Paolo Bergese, Laura Andreoli, Ugo D'Oro, Alessandra Zingoni, Angela Tincani, Silvano Sozzani, Daniela Bosisio
The excessive production of type I IFNs is a hallmark and a main pathogenic mechanism of many autoimmune diseases, including systemic lupus erythematosus (SLE). In these pathologies, the sustained secretion of type I IFNs is dependent on the improper activation of plasmacytoid DCs (pDCs) by self-nucleic acids. However, the nature and origin of pDC-activating self-nucleic acids is still incompletely characterized. Here, we report that exosomes isolated from the plasma of SLE patients can activate the secretion of IFN-α by human blood pDCs in vitro...
May 17, 2018: JCI Insight
https://www.readbyqxmd.com/read/29762500/circulating-micrornas-in-young-patients-with-acute-coronary-syndrome
#7
Kind-Leng Tong, Ahmad Syadi Mahmood Zuhdi, Wan Azman Wan Ahmad, Paul M Vanhoutte, Joao Pedro de Magalhaes, Mohd Rais Mustafa, Pooi-Fong Wong
Circulating microRNAs (miRNAs) hold great potential as novel diagnostic markers for acute coronary syndrome (ACS). This study sought to identify plasma miRNAs that are differentially expressed in young ACS patients (mean age of 38.5 ± 4.3 years) and evaluate their diagnostic potentials. Small RNA sequencing (sRNA-seq) was used to profile plasma miRNAs. Discriminatory power of the miRNAs was determined using receiver operating characteristic (ROC) analysis. Thirteen up-regulated and 16 down-regulated miRNAs were identified in young ACS patients...
May 15, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29760585/mir-93-5p-enhance-lacrimal-gland-adenoid-cystic-carcinoma-cell-tumorigenesis-by-targeting-brms1l
#8
Jie Hao, Xin Jin, Yan Shi, Hong Zhang
Background: Lacrimal adenoid cystic carcinoma (LACC) is one of the most common malignancies that affects lacrimal gland. MicroRNAs are known to play a crucial role as oncogenes or tumor suppressors. Specifically, miR-93 has been reported to play a crucial role in colorectal, breast, pancreatic, lung cancer and hepatocellular carcinoma. However, the role of miR-93 in LACC and the potential molecular mechanisms involved remain unknown. Therefore, we took the challenge to determine the involvement of miR-93 in the LACC by targeting BRMS1L...
2018: Cancer Cell International
https://www.readbyqxmd.com/read/29759067/small-rna-profiling-of-low-biomass-samples-identification-and-removal-of-contaminants
#9
Anna Heintz-Buschart, Dilmurat Yusuf, Anne Kaysen, Alton Etheridge, Joëlle V Fritz, Patrick May, Carine de Beaufort, Bimal B Upadhyaya, Anubrata Ghosal, David J Galas, Paul Wilmes
BACKGROUND: Sequencing-based analyses of low-biomass samples are known to be prone to misinterpretation due to the potential presence of contaminating molecules derived from laboratory reagents and environments. DNA contamination has been previously reported, yet contamination with RNA is usually considered to be very unlikely due to its inherent instability. Small RNAs (sRNAs) identified in tissues and bodily fluids, such as blood plasma, have implications for physiology and pathology, and therefore the potential to act as disease biomarkers...
May 14, 2018: BMC Biology
https://www.readbyqxmd.com/read/29752344/mir-4463-inhibits-the-migration-of-human-aortic-smooth-muscle-cells-by-amot
#10
Xueqin Wang, Chao Du, Xuemei He, Xian Deng, Yanzheng He, Xiangyu Zhou
Aberrant vascular smooth muscle cell (VSMC) migration has been implicated in a variety of vascular disorders, while the signal pathways governing this process remain unclear. Here, we investigated whether microRNAs (miRNAs), which are strong posttranscriptional regulators of gene expression, could alter VSMC migration. We detected the expression of miR-4463 in the plasma of patients with atherosclerosis and in human aortic smooth muscle cells (HASMCs) under hypoxia-ischemia condition, and investigated the migration effect and its downstream pathways...
May 11, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/29749537/exosome%C3%A2-encapsulated-microrna%C3%A2-23b-as-a-minimally-invasive-liquid-biomarker-for-the-prediction-of-recurrence-and-prognosis-of-gastric-cancer-patients-in-each-tumor-stage
#11
Yoshimasa Kumata, Hisae Iinuma, Yusuke Suzuki, Daisuke Tsukahara, Hironori Midorikawa, Yuichi Igarashi, Naruyoshi Soeda, Takashi Kiyokawa, Masahiro Horikawa, Ryoji Fukushima
Recently, exosome‑encapsulated microRNAs (miRNAs) have been attracting attention as stable and minimally invasive biomarkers in cancer patients. The aim of the present study was to clarify the value of plasma exosomal microRNA‑23b (miR‑23b) as a diagnostic and prognostic biomarker in gastric cancer (GC) patients at each tumor stage. We first selected recurrence specific exosomal miRNA by miRNA microarray from 6 GC patients (stage I) with or without recurrence, and 3 healthy volunteers. In this analysis, miR‑23b demonstrated the most significant change...
May 8, 2018: Oncology Reports
https://www.readbyqxmd.com/read/29749498/detection-of-mir%C3%A2-29a-in-plasma-of-patients-with-lumbar-spinal-stenosis-and-the-clinical-significance
#12
Genai Zhang, Wenping Zhang, Yu Hou, Yingchun Chen, Jipeng Song, Lixiang Ding
The present study aimed to detect miR‑29a expression in the plasma of patients with lumbar spinal stenosis (LSS) and to investigate the clinical significance. A total of 30 patients with LSS, 27 patients with lumbar intervertebral disc herniation (LDH), 27 healthy people and 7 patients that had succumbed to mortality were involved in the present study for specimen collection. Expression levels of miR‑29a in plasma and intervertebral disc tissue were detected by reverse transcription‑quantitative polymerase chain reaction analysis...
May 3, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29748623/plasma-microrna-panel-is-a-novel-biomarker-for-focal-segmental-glomerulosclerosis-and-associated-with-podocyte-apoptosis
#13
Bin Xiao, Li-Na Wang, Wei Li, Li Gong, Ting Yu, Qian-Fei Zuo, Hong-Wen Zhao, Quan-Ming Zou
Focal segmental glomerulosclerosis (FSGS) is a frequent glomerular disease, and is the common cause of nephrotic syndrome. However, there is no validated diagnostic blood biomarker for FSGS. Here, we performed a real-time PCR-based high-throughput miRNA profiling to identify the plasma signature for FSGS. We found four miRNAs (miR-17, miR-451, miR-106a, and miR-19b) were significantly downregulated in the plasma of FSGS patients (n = 97) compared with healthy controls (n = 124) in the training, validation, and blinded-test phases...
May 10, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29748371/mirna-590-3p-promotes-ovarian-cancer-growth-and-metastasis-via-a-novel-foxa2-versican-pathway
#14
Mohamed Salem, Jacob A O'Brien, Stefanie Bernaudo, Heba Shawer, Gang Ye, Jelena Brkić, Asma Amleh, Barbara C Vanderhyden, Basel Refky, Burton B Yang, Sergey N Krylov, Chun Peng
MicroRNAs (miRNAs) play important roles in gene regulation, and their dysregulation is associated with many diseases including epithelial ovarian cancer (EOC). In this study, we determined the expression and function of miR-590-3p in EOC. miR-590-3p levels were higher in high-grade carcinoma when compared to low-grade or tumors with low malignant potential. Interestingly, plasma levels of miR-590-3p were significantly higher in EOC patients than in subjects with benign gynecological disorders. Transient transfection of miR-590-3p mimics or stable transfection of mir-590 increased cell proliferation, migration, and invasion...
May 10, 2018: Cancer Research
https://www.readbyqxmd.com/read/29747626/differential-plasma-microvesicle-and-brain-profiles-of-microrna-in-experimental-cerebral-malaria
#15
Amy Cohen, Anna Zinger, Natalia Tiberti, Georges E R Grau, Valery Combes
BACKGROUND: Cerebral malaria (CM) is a fatal complication of Plasmodium infection, mostly affecting children under the age of five in the sub-Saharan African region. CM pathogenesis remains incompletely understood, although sequestered infected red blood cells, inflammatory cells aggregating in the cerebral blood vessels, and the microvesicles (MV) that they release in the circulation, have been implicated. Plasma MV numbers increase in CM patients and in the murine model, where blocking their release, genetically or pharmacologically, protects against brain pathology, suggesting a role of MV in CM neuropathogenesis...
May 11, 2018: Malaria Journal
https://www.readbyqxmd.com/read/29746939/identification-of-a-plasma-microrna-profile-in-untreated-pulmonary-tuberculosis-patients-that-is-modulated-by-anti-mycobacterial-therapy
#16
Barry Simone E, Ellis Magda, Yang YuRong, Guan Guangyu, Wang Xiaolin, Britton Warwick J, Saunders Bernadette M
OBJECTIVE: microRNA expression profiles are of interest as a biomarker of tuberculosis (TB). How anti-TB therapy effects miRNA profiles is unknown and was examined. METHODS: We identified 87 plasma miRNAs that were significantly modified in an exploratory group of 19 Chinese pulmonary TB (PTB) patients compared to 14 healthy controls. We selected 10 of these miRNAs for analysis in a cohort of 100 PTB patients prior to, and at one, two and six months during treatment...
May 7, 2018: Journal of Infection
https://www.readbyqxmd.com/read/29741193/identifying-exosome-derived-micrornas-as-candidate-biomarkers-of-frailty
#17
B R Ipson, M B Fletcher, S E Espinoza, A L Fisher
Frailty is a geriatric syndrome associated with progressive physical decline and significantly increases risk for falls, disability, hospitalizations, and death. However, much remains unknown regarding the biological mechanisms that contribute to aging and frailty, and to date, there are no clinically used prognostic or diagnostic molecular biomarkers. The present study profiled exosome-derived microRNAs isolated from the plasma of young, robust older, and frail older individuals and identified eight miRNAs that are uniquely enriched in frailty: miR-10a-3p, miR-92a-3p, miR-185-3p, miR-194-5p, miR-326, miR-532-5p, miR-576-5p, and miR-760...
2018: Journal of Frailty & Aging
https://www.readbyqxmd.com/read/29739113/combination-of-micrornas-and-cytokines-a-method-for-better-evaluation-of-acute-on-chronic-liver-failure
#18
Chunwen Pu, Chunmeng Jiang, Lang Lang, Huang Hui, Zhidong Wang, Dapeng Ma, Yong Zhang
BACKGROUND: The aim was to establish expression profiles of microRNAs (miRNAs) in Peripheral Blood Mononuclear Cells (PBMC) and of plasma cytokines from the patients with hepatitis B virus related acute-on-chronic liver failure (HBV-ACLF) and high-risk of acute-on-chronic liver failure (ACLF) patients as well as healthy people and to examine the relationships between these profiles and clinical features. METHODS: Herein, we collected PBMCs and plasma from peripheral blood of HBV-ACLF and ACLF patients as well as healthy people...
March 1, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/29739108/using-blood-and-plasma-micrornas-as-a-non-invasive-biomarker-in-patients-with-colorectal-cancer
#19
Horatiu Stefanescu, Delia Muntean, Ciprian Pilut, Mircea Diaconu, Roxana Popescu, Delia Hutanu, Marius Moise, Lighezan Diana, Razvan Nitu, Alina Popa Cherecheanu, Silvius Negoita, Ciprian V Iovan, Emil Ungureanu, Valentin Calu, Ioana Ciuca
BACKGROUND: A high percentage of oncological patients die yearly because of colorectal cancer (CRC). Worldwide, CRC represents the fourth leading cause of death among oncological patients. Numerous studies have been conducted in order to identify new biomarkers for the early diagnosis of patients with CRC. From this point of view, an ideal biomarker is represented by the expression of microRNAs. In this paper, we wish to summarize the expressions of microRNAs in CRC and to present the pathophysiological and genetic interactions that microRNAs have with protein systems in these patients...
March 1, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/29736829/circulating-microrna-22-3p-predicts-the-malignant-progression-of-precancerous-gastric-lesions-from-intestinal-metaplasia-to-early-adenocarcinoma
#20
Tsung-Hsing Chen, Cheng-Tang Chiu, Chieh Lee, Yin-Yi Chu, Hao-Tsai Cheng, Jun-Te Hsu, Ren-Chin Wu, Ta-Sen Yeh, Kwang-Huei Lin
BACKGROUND: Gastric cancer has a poor outcome and identifying useful biomarkers from peripheral blood or tissue could allow its early detection, or potentially precancerous changes, thus improving the curative rates. MicroRNAs (miRNAs) have been shown to offer great potential in cancer diagnosis and prediction. AIM: Here, we investigated the role of plasma miRNAs in the natural course of gastric cancer, from intestinal metaplasia to early cancer. The findings were used to understand whether patients at a high risk of malignancy could be given appropriate interventions in the early disease process, such as using endoscopic submucosal dissection to treat gastric dysplasia or early gastric cancer...
May 7, 2018: Digestive Diseases and Sciences
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