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Plasma microRNA

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https://www.readbyqxmd.com/read/29146158/plasma-microrna-155-5p-is-increased-among-patients-with-chronic-kidney-disease-and-nocturnal-hypertension
#1
Dominika Klimczak, Marek Kuch, Tomasz Pilecki, Dorota Żochowska, Agnieszka Wirkowska, Leszek Pączek
MicroRNAs play multiple roles in the regulation of blood pressure (BP). Nevertheless, to date, no study has assessed the association between microRNA plasma expression and BP control in chronic kidney disease (CKD) patients. Given this background, we evaluated the plasma expression of miR-155-5p, a translational inhibitor of angiotensin receptor type I, in CKD patients, to determine the association between miR-155-5p level and BP control. In this single-center cross-sectional study, we analyzed the miR-155-5p concentration by quantitative reverse transcriptase polymerase chain reaction using the U6 snRNA as a reference gene and 24-hour ambulatory blood pressure monitoring in CKD patients (stage ≥2) in relation to a control group of healthy age-matched and gender-matched individuals, with normal BP proven by the ambulatory blood pressure monitoring...
October 28, 2017: Journal of the American Society of Hypertension: JASH
https://www.readbyqxmd.com/read/29142030/plasma-microrna-profiles-in-patients-with-early-rheumatoid-arthritis-responding-to-adalimumab-plus-methotrexate-vs-methotrexate-alone-a-placebo-controlled-clinical-trial
#2
Jacob Sode, Sophine B Krintel, Anting Liu Carlsen, Merete L Hetland, Julia S Johansen, Kim Hørslev-Petersen, Kristian Stengaard-Pedersen, Torkell Ellingsen, Mark Burton, Peter Junker, Mikkel Østergaard, Niels H H Heegaard
OBJECTIVE: The aim was to identify plasma (i.e., cell-free) microRNA (miRNA) predicting antitumor necrosis and/or methotrexate (MTX) treatment response in patients enrolled in an investigator-initiated, prospective, double-blinded, placebo-controlled trial (The OPERA study, NCT00660647). METHODS: We included 180 disease-modifying antirheumatic drug-naive patients with early rheumatoid arthritis (RA) randomized to adalimumab (ADA; n = 89) or placebo (n = 91) in combination with MTX...
November 15, 2017: Journal of Rheumatology
https://www.readbyqxmd.com/read/29137394/measuring-plasma-levels-of-three-micrornas-can-improve-the-accuracy-for-identification-of-malignant-breast-lesions-in-women-with-bi-rads-4-mammography
#3
Julia Alejandra Pezuk, Thiago Luiz Araujo Miller, José Luiz Barbosa Bevilacqua, Alfredo Carlos Simões Dornellas de Barros, Felipe Eduardo Martins de Andrade, Luiza Freire de Andrade E Macedo, Vera Aguilar, Amanda Natasha Menardo Claro, Anamaria Aranha Camargo, Pedro Alexandre Favoretto Galante, Luiz F L Reis
A BI-RADS category of 4 from a mammogram indicates suspicious breast lesions, which require core biopsies for diagnosis and have an approximately one third chance of being malignant. Human plasma contains many circulating microRNAs, and variations in their circulating levels have been associated with pathologies, including cancer. Here, we present a novel methodology to identify malignant breast lesions in women with BI-RADS 4 mammography. First, we used the miRNome array and qRT-PCR to define circulating microRNAs that were differentially represented in blood samples from women with breast tumor (BI-RADS 5 or 6) in comparison to controls (BI-RADS 1 or 2)...
October 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/29136041/profiling-analysis-of-circulating-microrna-in-peripheral-blood-of-patients-with-class-iv-lupus-nephritis
#4
Elkin Navarro-Quiroz, Lisandro Pacheco-Lugo, Roberto Navarro-Quiroz, Hernan Lorenzi, Pierine España-Puccini, Yirys Díaz-Olmos, Lisneth Almendrales, Valeria Olave, Henry Gonzalez-Torres, Anderson Diaz-Perez, Alex Dominguez, Antonio Iglesias, Raul García, Gustavo Aroca-Martinez
Renal involvement in Systemic Lupus Erythematous (SLE) patients is one of the leading causes of morbidity and a significant contributor to mortality. It's estimated that nearly 50% of SLE individuals develop kidney disease in the first year of the diagnosis. Class IV lupus nephritis (LN-IV) is the class of lupus nephritis most common in Colombian patients with SLE. Altered miRNAs expression levels have been reported in human autoimmune diseases including lupus. Variations in the expression pattern of peripheral blood circulating miRNAs specific for this class of lupus nephritis could be correlated with the pathophysiological status of this group of individuals...
2017: PloS One
https://www.readbyqxmd.com/read/29134827/serum-plasma-and-saliva-biomarkers-for-head-and-neck-cancer
#5
Lidia Maria Rebolho Batista Arantes, Ana Carolina de Carvalho, Matias Eliseo Melendez, André Lopes Carvalho
Head and neck squamous cell carcinoma (HNSCC) encompasses tumors arising from several locations (oral and nasal cavities, paranasal sinuses, salivary glands, pharynx, and larynx) and currently stands as the sixth most common cancer worldwide. The most important risk factors identified so far are tobacco and alcohol consumption, and, for a subgroup of HNSCCs, infection with high-risk types of human papillomavirus (HPV). Despite several improvements in the treatment of these tumors in the last decades, overall survival rates have only improved marginally, mainly due to the advanced clinical stage at diagnosis and the high rates of treatment failure associated with this late diagnosis...
November 14, 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/29132874/combining-serum-microrna-and-ca-125-as-prognostic-indicators-of-preoperative-surgical-outcome-in-women-with-high-grade-serous-ovarian-cancer
#6
Jaynish S Shah, Gregory B Gard, Jean Yang, Jayne Maidens, Susan Valmadre, Patsy S Soon, Deborah J Marsh
OBJECTIVES: The most widely used approach for the clinical management of women with high-grade serous ovarian cancer (HGSOC) is surgery, followed by platinum and taxane based chemotherapy. The degree of macroscopic disease remaining at the conclusion of surgery is a key prognostic factor determining progression free and overall survival. We sought to develop a non-invasive test to assist surgeons to determine the likelihood of achieving complete surgical resection. This knowledge could be used to plan surgical approaches for optimal clinical management...
November 10, 2017: Gynecologic Oncology
https://www.readbyqxmd.com/read/29131834/plasma-microrna-profiling-exploring-better-biomarkers-for-lymphoma-surveillance
#7
Drirh Khare, Neta Goldschmidt, Aya Bardugo, Devorah Gur-Wahnon, Iddo Z Ben-Dov, Batia Avni
Early detection of relapsed lymphoma improves response and survival. Current tools lack power for detection of early relapse, while being cumbersome and expensive. We searched for sensitive biomarkers that precede clinical relapse, and serve for further studies on therapy response and relapse. We recruited 20 healthy adults, 14 diffuse large B-cell lymphoma (DLBCL) patients and 11 Hodgkin lymphoma (HL) patients at diagnosis. Using small-RNA sequencing we identified in DLBCL patients increased plasma levels of miR-124 and miR-532-5p, and decreased levels of miR-425, miR-141, miR-145, miR-197, miR-345, miR-424, miR-128 and miR-122...
2017: PloS One
https://www.readbyqxmd.com/read/29131249/study-on-the-expression-and-mechanism-of-plasma-microrna-21-in-patients-with-ischemic-cardiomyopathy
#8
M-B Xie, X-Q Sui, D Pei, Q Yao, Q Huang
OBJECTIVE: To investigate the expression and mechanism of plasma microRNA-21 in patients with ischemic cardiomyopathy (ICM). PATIENTS AND METHODS: 56 cases of ICM patients were selected in our hospital from February 2010 to March 2016 as the observation group, and 60 cases of healthy patients were selected as control group. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression of microRNA-21 in two groups. Then, differences of the total cholesterol (TC), triglycerides (TG), left ventricular ejection fraction (LVEF), high and low density lipoprotein cholesterol (HDL-C, LDL-C), left ventricular end-diastolic volume (LVEDV), N terminal B type brain natriuretic peptide (NT-proBNP) and other clinical indicators of the two groups, were compared...
October 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29131009/effects-of-microrna-499-on-the-inflammatory-damage-of-endothelial-cells-during-coronary-artery-disease-via-the-targeting-of-pdcd4-through-the-nf-%C3%AE-%C3%AE-tnf-%C3%AE-signaling-pathway
#9
Yu-Hong Zhang, Keng He, Gang Shi
BACKGROUND/AIMS: This study aimed to analyze the impact of microRNA-499 (miR-499) on the inflammatory damage of endothelial cells during coronary artery disease (CAD) via the targeting of PDCD4 through the NF-kB/ TNF-α signaling pathway. METHODS: A total of 216 CAD patients (CAD group) and 90 healthy people (normal group) were enrolled in our study. Endothelial cells were collected and assigned into normal, OX-LDL, negative control (NC), miR-499 inhibitor, miR-499 mimic, PDCD4 siRNA, and miR-499 inhibitor + PDCD4 siRNA groups...
November 6, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29130611/usefulness-of-exosome-encapsulated-microrna-451a-as-a-minimally-invasive-biomarker-for-prediction-of-recurrence-and-prognosis-in-pancreatic-ductal-adenocarcinoma
#10
Kunihiko Takahasi, Hisae Iinuma, Keita Wada, Shunryo Minezaki, Sachiyo Kawamura, Masahiko Kainuma, Yutaka Ikede, Makoto Shibuya, Fumihiko Miura, Keiji Sano
BACKGROUND: MicroRNAs (miRNAs) encapsulated in the exosomes of plasma is of interest as stable and minimally invasive biomarkers for recurrence and prognosis in cancer patients. The aim of this study was to clarify the predictive and prognostic value of plasma exosomal microRNA-451a (miR-451a) in patients with pancreatic ductal adenocarcinoma (PDAC). METHODS: Microarray-based expression profiling of miRNAs derived from exosomes in the plasma of 6 PDAC patients with UICC stage II was employed to identify a biomarker to distinguish between patients with and without recurrence...
November 11, 2017: Journal of Hepato-biliary-pancreatic Sciences
https://www.readbyqxmd.com/read/29126951/circulating-micrornas-as-novel-biomarkers-for-heart-failure
#11
Feng Chen, Jiefu Yang, Yingying Li, Hua Wang
BACKGROUND: The diagnosis of heart failure (HF) remains difficult. A wide variety of studies have indicated that microRNAs play important roles in the pathophysiology of HF. HYPOTHESIS: Circulating miRNAs may be the potential biomarkers for HF. METHODS: In this study, a genome-wide plasma miRNA expression analysis was performed in thirteen HF patients and three controls. Microarray was used for the initial screen, followed by validation with quantitative real-time PCR (qRT-PCR) assays in an independent cohort of thirty-three HF patients and twenty controls...
November 7, 2017: Hellenic Journal of Cardiology: HJC, Hellēnikē Kardiologikē Epitheōrēsē
https://www.readbyqxmd.com/read/29125182/circulating-micrornas-as-potential-biomarkers-in-myasthenia-gravis-patients
#12
REVIEW
Anna Rostedt Punga, Tanel Punga
MicroRNAs (miRNAs) are small noncoding RNA molecules that bind to specific mRNA targets and regulate a wide range of important biological processes within cells. Circulating miRNAs are released into the extracellular space and can be measured in most biofluids, including blood serum and plasma. Recently, circulating miRNAs have emerged as easily accessible markers in various body fluids with different profiles and quantities specific for different human disorders, including autoimmune diseases. In myasthenia gravis (MG), diagnostic tests such as titers of serum autoantibodies specific for either the acetylcholine receptor (AChR(+) ) or muscle-specific tyrosine kinase (MuSK(+) ) do not necessarily reflect disease progression, and there is a great need for reliable objective biomarkers to monitor the disease course and therapeutic response...
November 10, 2017: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/29122890/microrna-30c-reduces-plasma-cholesterol-in-homozygous-familial-hypercholesterolemic-and-type-2-diabetic-mouse-models
#13
Sara Irani, Jahangir Iqbal, W James Antoni, Laraib Ijaz, M Mahmood Hussain
High plasma cholesterol levels are found in several metabolic disorders and their reductions are advocated to reduce risk of atherosclerosis. A way to lower plasma lipids is to curtail lipoprotein production; however, this is associated with steatosis. We previously showed that microRNA-30c (miR-30c) lowers diet-induced hypercholesterolemia and atherosclerosis in C57BL/6J and Apoe-/- mice. Here, we tested miR-30c effect on plasma lipids, transaminases and hepatic lipids in different mouse models. Hepatic delivery of miR-30c to chow fed leptin deficient (ob/ob) and leptin receptor deficient (db/db) hypercholesterolemic and hyperglycemic mice reduced cholesterol in total plasma and VLDL/LDL by ~28% and ~25%, respectively, without affecting triglyceride and glucose levels...
November 9, 2017: Journal of Lipid Research
https://www.readbyqxmd.com/read/29121998/circulating-brain-enriched-micrornas-as-novel-biomarkers-for-detection-and-differentiation-of-neurodegenerative-diseases
#14
Kira S Sheinerman, Jon B Toledo, Vladimir G Tsivinsky, David Irwin, Murray Grossman, Daniel Weintraub, Howard I Hurtig, Alice Chen-Plotkin, David A Wolk, Leo F McCluskey, Lauren B Elman, John Q Trojanowski, Samuil R Umansky
BACKGROUND: Minimally invasive specific biomarkers of neurodegenerative diseases (NDs) would facilitate patient selection and disease progression monitoring. We describe the assessment of circulating brain-enriched microRNAs as potential biomarkers for Alzheimer's disease (AD), frontotemporal dementia (FTD), Parkinson's disease (PD), and amyotrophic lateral sclerosis (ALS). METHODS: In this case-control study, the plasma samples were collected from 250 research participants with a clinical diagnosis of AD, FTD, PD, and ALS, as well as from age- and sex-matched control subjects (n = 50 for each group), recruited from 2003 to 2015 at the University of Pennsylvania Health System, including the Alzheimer's Disease Center, the Parkinson's Disease and Movement Disorders Center, the Frontotemporal Degeneration Center, and the Amyotrophic Lateral Sclerosis Clinic...
November 9, 2017: Alzheimer's Research & Therapy
https://www.readbyqxmd.com/read/29118269/genetic-architecture-and-regulatory-impact-on-hepatic-microrna-expression-linked-to-immune-and-metabolic-traits
#15
Siriluck Ponsuksili, Nares Trakooljul, Frieder Hadlich, Fiete Haack, Eduard Murani, Klaus Wimmers
Regulation of microRNA (miRNA) expression contributes to a wide range of target gene expression and phenotypes. The miRNA expression in the liver, the central metabolic organ, was examined in 209 pigs, and integrated with haematological and clinical biomarkers of metabolic and overall health, mRNA-target expression levels and single-nucleotide polymorphism (SNP) genotypes. The expression levels of 426 miRNA species correlated with plasma haematological or biochemical traits (r² = |0.19-0.45|, false discovery rate < 5%)...
November 2017: Open Biology
https://www.readbyqxmd.com/read/29116107/micrornas-regulating-superoxide-dismutase-2-are-new-circulating-biomarkers-of-heart-failure
#16
Emilie Dubois-Deruy, Marie Cuvelliez, Jan Fiedler, Henri Charrier, Paul Mulder, Eleonore Hebbar, Angelika Pfanne, Olivia Beseme, Maggy Chwastyniak, Philippe Amouyel, Vincent Richard, Christophe Bauters, Thomas Thum, Florence Pinet
Although several risk factors such as infarct size have been identified, the progression of heart failure (HF) remains difficult to predict in clinical practice. Using an experimental rat model of post-myocardial infarction (MI), we previously identified 45 proteins differentially modulated during HF by proteomic analysis. This study sought to identify microRNAs (miRNAs) able to regulate these proteins and to test their relevance as biomarkers for HF. In silico bioinformatical analysis selected 13 miRNAs related to the 45 proteins previously identified...
November 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29115644/identification-of-differentially-expressed-micrornas-in-acute-kawasaki-disease
#17
Ye Chen, Yue-Yue Ding, Yan Ren, Lei Cao, Qiu-Qin Xu, Ling Sun, Ming-Guo Xu, Hai-Tao Lv
The present study used microarray analysis to screen the plasma expression of microRNAs (miRNAs) in patients with acute Kawasaki disease (KD) and aimed to explore the pathogenesis of KD. Plasma was collected from children with acute KD (n=6) and from healthy control children (n=6). Total RNA was extracted and differential miRNA expression between the two groups was determined. Differentially expressed miRNAs were validated using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) in an independent cohort (n=8)...
November 8, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29114076/circular-rna-dlgap4-ameliorates-ischemic-stroke-outcomes-by-targeting-mir-143-to-regulate-endothelial-mesenchymal-transition-associated-with-blood-brain-barrier-integrity
#18
Ying Bai, Yuan Zhang, Bing Han, Li Yang, Xufeng Chen, Rongrong Huang, Fangfang Wu, Jie Chao, Pei Liu, Gang Hu, John H Zhang, Honghong Yao
Circular RNAs (circRNAs) are highly expressed in the central nervous system and regulate physiological and pathophysiological processes. However, the potential role of circRNAs in stroke remains largely unknown. Here, we show that the circular RNA DLGAP4 (circDLGAP4) functions as an endogenous microRNA-143 (miR-143) sponge to inhibit miR-143 activity, resulting in the inhibition of HECT domain E3 ubiquitin protein ligase 1 (HECTD1) expression. CircDLGAP4 levels were significantly decreased in the plasma of acute ischemic stroke patients (13 females and 13 males) and in a mouse stroke model...
November 7, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/29107578/facile-detection-of-microrna-based-on-phosphorescence-resonance-energy-transfer-and-duplex-specific-nuclease-assisted-signal-amplification
#19
Jia-Jia Yang, Zhi-Feng Zhang, Gui-Qin Yan
MicroRNAs (miRNAs) play an important role in many biological processes, and its level in plasma and other biological fluids is closely related to many diseases. In this work, a selective room-temperature phosphorescence (RTP) detection method for miRNA was developed based on a duplex-specific nuclease (DSN) -assisted signal amplification strategy and phosphorescence resonance energy transfer (PRET) between poly-diallyldimethylammonium chloride-modified quantum dots (QDs@PDDA) and 6-carboxy-X-rhodamine-modified miRNA sequences complementary oligonucleotide (ROX-ssDNA)...
October 28, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/29105802/circulating-micrornas-and-treatment-response-in-the-phase-ii-swog-s0925-study-for-patients-with-new-metastatic-hormone-sensitive-prostate-cancer
#20
Heather H Cheng, Melissa Plets, Hongli Li, Celestia S Higano, Catherine M Tangen, Neeraj Agarwal, Nicholas J Vogelzang, Maha Hussain, Ian M Thompson, Muneesh Tewari, Evan Y Yu
BACKGROUND: Previous studies suggest circulating, blood-based microRNAs (miRNAs) may serve as minimally invasive prostate cancer biomarkers, however there is limited data from prospective clinical trials. Here, we explore the role of candidate plasma miRNAs as potential biomarkers in the SWOG 0925 randomized phase II study of androgen deprivation combined with cixutumumab versus androgen deprivation alone in patients with new metastatic hormone-sensitive prostate cancer. METHODS: Correlative biospecimens, including circulating tumor cells (CTCs) and plasma for miRNA analysis, were collected at baseline and after 12 weeks on treatment from 50 patients enrolled on SWOG 0925...
November 6, 2017: Prostate
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