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Plasma microRNA

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https://www.readbyqxmd.com/read/28550083/normal-and-cancerous-tissues-release-extrachromosomal-circular-dna-eccdna-into-the-circulation
#1
Pankaj Kumar, Laura Dillon, Yoshiyuki Shibata, Amir A Jazaeri, David R Jones, Anindya Dutta
Cell-free circulating linear DNA is being explored for non-invasive diagnosis and management of tumors and fetuses, the so-called liquid biopsy. Previously, we observed the presence of small extrachromosomal circular DNA (eccDNA), called microDNA, in the nuclei of mammalian tissues and cell lines. Now, we demonstrate that cell-free microDNA derived from uniquely mapping regions of the genome is detectable in plasma and serum from both mice and humans and that they are significantly longer (30-60% >250 bases) than cell-free circulating linear DNA (~150 bases)...
May 26, 2017: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/28546328/microrna-15a-16-1-antagomir-ameliorates-ischemic-brain-injury-in-experimental-stroke
#2
Xinxin Yang, Xuelian Tang, Ping Sun, Yejie Shi, Kai Liu, Sulaiman H Hassan, R Anne Stetler, Jun Chen, Ke-Jie Yin
BACKGROUND AND PURPOSE: Dysregulation of the miR-15a/16-1 cluster in plasma has been reported in patients with stroke as a potential biomarker for diagnostic and prognostic use. However, the essential role and therapeutic potential of the miR-15a/16-1 cluster in ischemic stroke are poorly understood. This study is aimed at investigating the regulatory role of the miR-15a/16-1 cluster in ischemic brain injury and insight mechanisms. METHODS: Adult male miR-15a/16-1 knockout and wild-type mice, or adult male C57 BL/6J mice injected via tail vein with the miR-15a/16-1-specific inhibitor (antagomir, 30 pmol/g), were subjected to 1 hour of middle cerebral artery occlusion and 72 hours of reperfusion...
May 25, 2017: Stroke; a Journal of Cerebral Circulation
https://www.readbyqxmd.com/read/28542740/plasma-micrornas-as-biomarkers-of-pancreatic-cancer-risk-in-a-prospective-cohort-study
#3
Eric J Duell, Leila Lujan-Barroso, Núria Sala, Samantha Deitz McElyea, Kim Overvad, Anne Tjonneland, Anja Olsen, Elisabete Weiderpass, Lill-Tove Busund, Line Moi, David Muller, Paolo Vineis, Dagfinn Aune, Giuseppe Matullo, Alessio Naccarati, Salvatore Panico, Giovanna Tagliabue, Rosario Tumino, Domenico Palli, Rudolf Kaaks, Verena A Katzke, Heiner Boeing, H B As Bueno-de-Mesquita, Petra H Peeters, Antonia Trichopoulou, Pagona Lagiou, Anastasia Kotanidou, Ruth C Travis, Nick Wareham, Kay-Tee Khaw, Jose Ramon Quiros, Miguel Rodríguez-Barranco, Miren Dorronsoro, María-Dolores Chirlaque, Eva Ardanaz, Gianluca Severi, Marie-Christine Boutron-Ruault, Vinciane Rebours, Paul Brennan, Marc Gunter, Ghislaine Scelo, Greg Cote, Stuart Sherman, Murray Korc
Non-invasive biomarkers for early pancreatic ductal adenocarcinoma (PDAC) diagnosis and disease risk stratification are greatly needed. We conducted a nested case-control study within the Prospective Investigation into Cancer and Nutrition (EPIC) cohort to evaluate pre-diagnostic microRNAs (miRs) as biomarkers of subsequent PDAC risk. A panel of eight miRs (miR-10a, -10b, -21-3p, -21-5p, -30c, -106b, -155, and -212) based on previous evidence from our group was evaluated in 225 microscopically confirmed PDAC cases and 225 controls matched on center, sex, fasting status, and age/date/time of blood collection...
May 19, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28540980/low-expression-of-circulating-microrna-34c-is-associated-with-poor-prognosis-in-triple-negative-breast-cancer
#4
Zhihao Zeng, Xiaowu Chen, Dajian Zhu, Zhongran Luo, Min Yang
PURPOSE: The microRNA-34 (miR-34) family is important in tumor regulation. This study aimed to investigate the association of circulating miR-34 family proteins with clinicopathological features and their prognostic value in triple-negative breast cancer (TNBC) patients. MATERIALS AND METHODS: In this cohort study, 173 TNBC patients admitted to First People's Hospital of Shunde from May 1, 2009 to April 30, 2013 were enrolled. Meanwhile, 75 age-matched healthy women volunteers were identified as healthy controls (HCs)...
July 2017: Yonsei Medical Journal
https://www.readbyqxmd.com/read/28540642/plasma-and-white-blood-cells-show-different-mirna-expression-profiles-in-parkinson-s-disease
#5
Christine Schwienbacher, Luisa Foco, Anne Picard, Eloina Corradi, Alice Serafin, Jörg Panzer, Stefano Zanigni, Hagen Blankenburg, Maurizio F Facheris, Giulia Giannini, Marika Falla, Pietro Cortelli, Peter P Pramstaller, Andrew A Hicks
Parkinson's disease (PD) diagnosis is based on the assessment of motor symptoms, which manifest when more than 50% of dopaminergic neurons are degenerated. To date, no validated biomarkers are available for the diagnosis of PD. The aims of the present study are to evaluate whether plasma and white blood cells (WBCs) are interchangeable biomarker sources and to identify circulating plasma-based microRNA (miRNA) biomarkers for an early detection of PD. We profiled plasma miRNA levels in 99 L-dopa-treated PD patients from two independent data collections, in ten drug-naïve PD patients, and in unaffected controls matched by sex and age...
May 24, 2017: Journal of Molecular Neuroscience: MN
https://www.readbyqxmd.com/read/28540143/association-between-coronary-artery-disease-and-microrna-literature-review-and-clinical-perspective
#6
Rehan Malik, Raja S Mushtaque, Usman A Siddiqui, Adnan Younus, Muhammad A Aziz, Choudhry Humayun, Kanaan Mansoor, Muhammad A Latif, Salman Waheed, Salman Assad, Idrees Khan, Syed M Bukhari, Daniel DelCampo, Ali Adus, Swetha Gannarapu
BACKGROUND: Until recently, circulating micro-RNAs (miRNAs) have attracted major interest as novel biomarkers for the early diagnosis of coronary artery disease (CAD). This review article summarizes the available evidence on the correlation of micro-RNAs with both the clinical and subclinical coronary artery disease and highlights the necessity for exploring miRNAs as a potential diagnostic and prognostic biomarker of early CAD in an adult population. METHODS: A systematic literature analysis and retrieval online systems Public/Publisher MEDLINE/ Excerpta Medica Database /Medical Literature Analysis and Retrieval System Online,(PUBMED/EMBASE/MEDLINE) search were conducted for relevant information...
April 23, 2017: Curēus
https://www.readbyqxmd.com/read/28539664/mirna-29c-regulates-the-expression-of-inflammatory-cytokines-in-diabetic-nephropathy-by-targeting-tristetraprolin
#7
Jia Guo, Jing Li, Jing Zhao, Shuguang Yang, Luyao Wang, Genyang Cheng, Dong Liu, Jing Xiao, Zhangsuo Liu, Zhanzheng Zhao
Diabetic nephropathy is one of the most prevalent chronic complications of Diabetes mellitus, but its pathogenesis remains elusive. This study was designed to determine the role of tristetraprolin (TTP), inflammatory cytokines and microRNAs (miRNAs) in DN. The blood and urine samples were obtained from 32 patients with DN, 33 patients with type 2 DM, and 35 normal healthy subjects as controls. Renal tissue samples were also obtained from 10 DN patients and 10 normal controls. The miRNA microarray analyses were performed in pooled plasma and urine sediment samples of eight DN patients and eight age- and sex-matched health control subjects and three paired renal tissues from patients with DN and normal controls...
May 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28536858/quantification-of-micrornas-directly-from-body-fluids-using-a-base-stacking-isothermal-amplification-method-in-a-point-of-care-device
#8
Maggie R Williams, Robert D Stedtfeld, Tiffany M Stedtfeld, James M Tiedje, Syed A Hashsham
MicroRNAs have been proposed to be a class of biomarkers of disease as expression levels are significantly altered in various tissues and body fluids when compared to healthy controls. As such, the detection and quantification of microRNAs is imperative. While many methods have been established for quantification of microRNAs, they typically rely on time consuming handling such as RNA extraction, purification, or ligation. Here we describe a novel method for quantification of microRNAs using direct amplification in body fluids without upstream sample preparation...
September 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28536479/circulating-microrna-214-and-126-as-potential-biomarkers-for-canine-neoplastic-disease
#9
Kazuki Heishima, Yukie Ichikawa, Kyoko Yoshida, Ryota Iwasaki, Hiroki Sakai, Takayuki Nakagawa, Yuiko Tanaka, Yuki Hoshino, Yasuhiko Okamura, Mami Murakami, Kohji Maruo, Yukihiro Akao, Takashi Mori
Circulating microRNAs in the blood may provide diagnostic and prognostic information about canine neoplastic diseases, and their profiles may be conserved between human and canine species. We performed RT-qPCR to obtain the profiles of circulating plasma microRNA-214 and -126 in total 181 cases of canine neoplastic diseases and healthy controls. MicroRNA-214 levels were high in 2 epithelial tumours (thyroid and mammary carcinomas) and 4 non-epithelial tumours (osteosarcoma, histiocytic sarcoma, chondrosarcoma, and hemangiosarcoma)...
May 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28536008/chemosensitization-and-inhibition-of-pancreatic-cancer-stem-cell-proliferation-by-overexpression-of-microrna-205
#10
Amit Kumar Chaudhary, Goutam Mondal, Virender Kumar, Krishna Kattel, Ram I Mahato
Treatment of pancreatic cancer with gemcitabine (GEM) is limited due to its rapid plasma metabolism and development of chemoresistance. MicroRNA (miRNA) regulates cancer stem cell (CSC) maintenance and induces chemoresistance in cancer cells. In this study, we observed differential downregulation of miR-205 (miR-205-5p) in human pancreatic cancer tissues and cells. Compared to GEM-sensitive MIA PaCa-2 cells, miR-205 was highly downregulated in GEM-resistant MIA PaCa-2R cells. Lentivirus-mediated overexpression of miR-205 inhibits MIA PaCa-2R cell proliferation after GEM-treatment...
May 20, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28534160/micrornas-new-therapeutic-targets-for-familial-hypercholesterolemia
#11
REVIEW
Amir Abbas Momtazi, Maciej Banach, Matteo Pirro, Evan A Stein, Amirhossein Sahebkar
Familial hypercholesterolemia (FH) is the most common inherited form of dyslipidemia and a major cause of premature cardiovascular disease. Management of FH mainly relies on the efficiency of treatments that reduce plasma low-density lipoprotein (LDL) cholesterol (LDL-C) concentrations. MicroRNAs (miRs) have been suggested as emerging regulators of plasma LDL-C concentrations. Notably, there is evidence showing that miRs can regulate the post-transcriptional expression of genes involved in the pathogenesis of FH, including LDLR, APOB, PCSK9, and LDLRAP1...
May 22, 2017: Clinical Reviews in Allergy & Immunology
https://www.readbyqxmd.com/read/28531338/placental-exosomes-as-early-biomarker-of-preeclampsia-potential-role-of-exosomal-micrornas-across-gestation
#12
Carlos Salomon, Dominic Guanzon, Katherin Scholz-Romero, Sherri Longo, Paula Correa, Sebastian E Illanes, Gregory E Rice
Context: There is a need to develop strategies for early prediction of patients who will develop preeclampsia (PE), in order to establish preventive strategies to reduce the prevalence and severity of the disease and their associated complications. Objective: The objective of this study was to investigate whether exosomes and their microRNA cargo present in maternal circulation can be used as early biomarker for PE. Design, Setting, Patients, and Interventions: A retrospective stratified study design was used to quantify total exosomes (EXO) and placenta-derived exosomes (PdE) present in maternal plasma of normal (n=32 per time point) and PE (n=15 per time point) pregnancies...
May 22, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28526870/time-course-responses-of-circulating-micrornas-to-three-resistance-training-protocols-in-healthy-young-men
#13
Shufang Cui, Biao Sun, Xin Yin, Xia Guo, Dingming Chao, Chunni Zhang, Chen-Yu Zhang, Xi Chen, Jizheng Ma
Circulating microRNAs (c-miRNAs) in human plasma have been described as a potential marker of exercise. The present study investigated the effects of three acute resistance training (RT) protocols on the time-course changes of the c-miRNAs profiles in young males. The subjects (n = 45) were randomly divided into three groups: muscular strength endurance (SE), muscular hypertrophy (MH) and maximum strength (MS). Venous blood samples were obtained before exercise and immediately, 1 h and 24 h after each RT protocol to assess the following biological parameters: c-miRNAs, anabolic and catabolic hormones, inflammatory cytokines and muscle damage markers...
May 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28523996/micro-rnas-exercise-and-cellular-plasticity-in-humans-the-impact-of-dietary-factors-and-hypoxia
#14
Timothée Watier, Anthony Mj Sanchez
Physical exercise is a stress that modulates a plethora of cellular mechanisms to promote morphological and metabolic adaptations. Substantial efforts have been made to better understand the modulation of pathways involved in cell plasticity during exercise, but human explorations may be limited because invasive procedures are often required. In recent years, minimally invasive methods for the analysis of microRNAs (miRNAs) modulation in response to exercise have been developed. miRNAs are small molecules that belong to a family of non-coding RNAs...
May 19, 2017: MicroRNA
https://www.readbyqxmd.com/read/28521416/a-proposed-method-for-the-relative-quantification-of-levels-of-circulating-micrornas-in-the-plasma-of-gastric-cancer-patients
#15
Mayra-Cecilia Suárez-Arriaga, Javier Torres, Margarita Camorlinga-Ponce, Alejandro Gómez-Delgado, Patricia Piña-Sánchez, Hilda-Alicia Valdez-Salazar, Rosa-María Ribas-Aparicio, Ezequiel M Fuentes-Pananá, Martha-Eugenia Ruiz-Tachiquín
Gastric cancer (GC) is the fifth most common type of malignancy and the third leading cause of cancer-associated mortality worldwide. It is necessary to identify novel methods aimed at improving the early diagnosis and treatment of GC. MicroRNA expression profiles in the plasma of patients with GC have demonstrated a potential use in the opportune diagnosis of this neoplasm. However, there are currently no standardized targets for use in the normalization of microRNA Cq values for different neoplasms. The present study tested two normalization approaches while analyzing plasma derived from patients with GC and non-atrophic gastritis...
May 2017: Oncology Letters
https://www.readbyqxmd.com/read/28514730/circulating-micrornas-as-novel-biomarkers-of-alk-positive-nonsmall-cell-lung-cancer-and-predictors-of-response-to-crizotinib-therapy
#16
Liang-Liang Li, Li-Li Qu, Han-Jiang Fu, Xiao-Fei Zheng, Chuan-Hao Tang, Xiao-Yan Li, Jian Chen, Wei-Xia Wang, Shao-Xing Yang, Lin Wang, Guan-Hua Zhao, Pan-Pan Lv, Min Zhang, Yang-Yang Lei, Hai-Feng Qin, Hong Wang, Hong-Jun Gao, Xiao-Qing Liu
Circulating microRNAs are potential diagnostic and predictive biomarkers, but have not been investigated for patients with anaplastic lymphoma kinase (ALK)-positive lung cancer. In this exploratory study, we sought to identify potential plasma biomarkers for ALK-positive non-small cell lung cancer (NSCLC). A microRNA microarray was used to select ALK-related microRNAs in ALK-positive NSCLC (n = 3), ALK-negative NSCLC (n = 3), and healthy subjects (n = 3). Plasma levels of 21 microRNAs were differentially expressed for ALK-positive and ALK-negative NSCLC, including 14 down-regulated and 7 up-regulated microRNAs...
April 30, 2017: Oncotarget
https://www.readbyqxmd.com/read/28513859/identification-of-a-plasma-mirna-biomarker-signature-for-allergic-asthma-a-translational-approach
#17
Katrin Milger, Jeremias Götschke, Linda Krause, Petra Nathan, Francesca Alessandrini, Amanda Tufman, Rainald Fischer, Sabine Bartel, Fabian J Theis, Jürgen Behr, Stefan Dehmel, Nikola S Mueller, Nikolaus Kneidinger, Susanne Krauss-Etschmann
BACKGROUND: Asthma is a heterogeneous chronic disease with different phenotypes and treatment responses. Thus, there is a high clinical need for molecular disease biomarkers to aid in differentiating these distinct phenotypes. As MicroRNAs (miRNAs), that regulate gene expression at the post-transcriptional level, are altered in experimental and human asthma, circulating miRNAs are attractive candidates for the identification of novel biomarkers. This study aimed to identify plasmatic miRNA-based biomarkers of asthma, through a translational approach...
May 17, 2017: Allergy
https://www.readbyqxmd.com/read/28511692/vitamin-d-receptor-activation-reduces-inflammatory-cytokines-and-plasma-micrornas-in-moderate-chronic-kidney-disease-a-randomized-trial
#18
Ladan Mansouri, Kristina Lundwall, Ali Moshfegh, Stefan H Jacobson, Joachim Lundahl, Jonas Spaak
BACKGROUND: Chronic kidney disease (CKD) is a major risk factor for cardiovascular disease (CVD), partly due to endothelial dysfunction and chronic inflammation. Vitamin D treatment in end stage renal disease is suggested to modulate the immune system and lead to improved outcomes. We and others have demonstrated that treatment with vitamin D or activated vitamin D analogues protects the endothelial function in less severe renal disease as well. Since the endothelial protection might be mediated by vitamin D effects on inflammation, we assessed levels of pro-inflammatory cytokines and micro RNAs (miRs) in patients with moderate CKD, treated with an active vitamin D analogue (paricalcitol)...
May 16, 2017: BMC Nephrology
https://www.readbyqxmd.com/read/28502057/microrna-125b-in-peripheral-blood-a-potential-biomarker-for-severity-and-prognosis-of-children-with-viral-encephalitis
#19
Qin-Ling Gao, Yun-Xia Ma, Da-Wei Yuan, Qing-Cai Zhang, Jun Zeng, Hao Li
This study aims to evaluate the effect of peripheral blood miR-125b expression on severity and prognosis in children with viral encephalitis (VE). Children with VE (severe and mild groups) were grouped into VE group, and 40 healthy children as control group. Plasma RNA was extracted, and real-time quantitative PCR was conducted to detect miR-125b relative expression. Associations of miR-125b expression with clinical characteristics and prognosis of VE children were analyzed. Area under ROC curve (AUC) was calculated to evaluate the accuracy of the prognostic value of miR-125b...
May 13, 2017: Neurological Sciences
https://www.readbyqxmd.com/read/28500171/platelet-microparticles-infiltrating-solid-tumors-transfer-mirnas-that-suppress-tumor-growth
#20
James V Michael, Jeremy G T Wurtzel, Guang Fen Mao, A Koneti Rao, Mikhail A Kolpakov, Abdelkarim Sabri, Nicholas E Hoffman, Sudarsan Rajan, Dhanendra Tomar, Muniswamy Madesh, Marvin T Nieman, Johnny Yu, Leonard C Edelstein, Jesse W Rowley, Andrew S Weyrich, Lawrence E Goldfinger
Platelet-derived microparticles (PMPs) are associated with enhancement of metastasis and poor cancer outcomes. Circulating PMPs transfer platelet microRNAs (miRNAs) to vascular cells. Solid tumor vasculature is highly permeable, allowing the possibility of PMP-tumor cell interaction. Here we show that PMPs infiltrate solid tumors in humans and mice and transfer platelet-derived RNA, including miRNAs, to tumor cells in vivo and in vitro, resulting in tumor cell apoptosis. MiR-24 was a major species in this transfer...
May 12, 2017: Blood
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