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Plasma Mir

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https://www.readbyqxmd.com/read/29146158/plasma-microrna-155-5p-is-increased-among-patients-with-chronic-kidney-disease-and-nocturnal-hypertension
#1
Dominika Klimczak, Marek Kuch, Tomasz Pilecki, Dorota Żochowska, Agnieszka Wirkowska, Leszek Pączek
MicroRNAs play multiple roles in the regulation of blood pressure (BP). Nevertheless, to date, no study has assessed the association between microRNA plasma expression and BP control in chronic kidney disease (CKD) patients. Given this background, we evaluated the plasma expression of miR-155-5p, a translational inhibitor of angiotensin receptor type I, in CKD patients, to determine the association between miR-155-5p level and BP control. In this single-center cross-sectional study, we analyzed the miR-155-5p concentration by quantitative reverse transcriptase polymerase chain reaction using the U6 snRNA as a reference gene and 24-hour ambulatory blood pressure monitoring in CKD patients (stage ≥2) in relation to a control group of healthy age-matched and gender-matched individuals, with normal BP proven by the ambulatory blood pressure monitoring...
October 28, 2017: Journal of the American Society of Hypertension: JASH
https://www.readbyqxmd.com/read/29145255/functional-polymorphism-rs3783553-in-the-3-untranslated-region-of-il-1a-increased-the-risk-of-ischemic-stroke-a-case-control-study
#2
Peng Wang, Qian He, Chen Liu, Shi Zhen He, Shou Yan Zhu, Ying Wen Li, Wei Su, Shu Tian Xiang, Bo Zhao
Accumulating evidence indicates interleukin-1 (IL-1) is a critical mediator of inflammatory responses in ischemic stroke (IS). The aim of this study was to investigate whether rs3783553 in the 3'-untranslated region of IL-1A was associated with the risk of IS. In this hospital-based case-control study, we genotyped the rs3783553 using polymerase chain reaction in 316 patients with IS and 332 age, sex, and ethnicity-matched controls. Plasma level of IL-1α was measured by enzyme-linked immunosorbent assay. The relative luciferase activities were measured by the Dual Luciferase assay system...
November 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29142572/clinicopathological-features-associated-to-mirna-195-expression-in-patients-with-breast-cancer-evidence-of-a-potential-biomarker
#3
Farkhunda Nadeem, Muhammad Hanif, Akhtar Ahmed, Qamar Jamal, Adnan Khan
Background & Objective: MiRNAs are a systematic class of small non-coding RNAs with impending role as tumor biomarkers. Our objective was to identify the level of expression of Mir-195 in patients with breast cancer along with its correlation with clinicopathologic features. Methods: A total of 209 females in which 139 histologically diagnosed breast cancer (BC) cases and 70 healthy controls matched for age, their relative clinical and histopathological findings were recorded from their laboratory reports and hospital record of the patients...
September 2017: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/29142030/plasma-microrna-profiles-in-patients-with-early-rheumatoid-arthritis-responding-to-adalimumab-plus-methotrexate-vs-methotrexate-alone-a-placebo-controlled-clinical-trial
#4
Jacob Sode, Sophine B Krintel, Anting Liu Carlsen, Merete L Hetland, Julia S Johansen, Kim Hørslev-Petersen, Kristian Stengaard-Pedersen, Torkell Ellingsen, Mark Burton, Peter Junker, Mikkel Østergaard, Niels H H Heegaard
OBJECTIVE: The aim was to identify plasma (i.e., cell-free) microRNA (miRNA) predicting antitumor necrosis and/or methotrexate (MTX) treatment response in patients enrolled in an investigator-initiated, prospective, double-blinded, placebo-controlled trial (The OPERA study, NCT00660647). METHODS: We included 180 disease-modifying antirheumatic drug-naive patients with early rheumatoid arthritis (RA) randomized to adalimumab (ADA; n = 89) or placebo (n = 91) in combination with MTX...
November 15, 2017: Journal of Rheumatology
https://www.readbyqxmd.com/read/29137394/measuring-plasma-levels-of-three-micrornas-can-improve-the-accuracy-for-identification-of-malignant-breast-lesions-in-women-with-bi-rads-4-mammography
#5
Julia Alejandra Pezuk, Thiago Luiz Araujo Miller, José Luiz Barbosa Bevilacqua, Alfredo Carlos Simões Dornellas de Barros, Felipe Eduardo Martins de Andrade, Luiza Freire de Andrade E Macedo, Vera Aguilar, Amanda Natasha Menardo Claro, Anamaria Aranha Camargo, Pedro Alexandre Favoretto Galante, Luiz F L Reis
A BI-RADS category of 4 from a mammogram indicates suspicious breast lesions, which require core biopsies for diagnosis and have an approximately one third chance of being malignant. Human plasma contains many circulating microRNAs, and variations in their circulating levels have been associated with pathologies, including cancer. Here, we present a novel methodology to identify malignant breast lesions in women with BI-RADS 4 mammography. First, we used the miRNome array and qRT-PCR to define circulating microRNAs that were differentially represented in blood samples from women with breast tumor (BI-RADS 5 or 6) in comparison to controls (BI-RADS 1 or 2)...
October 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/29136041/profiling-analysis-of-circulating-microrna-in-peripheral-blood-of-patients-with-class-iv-lupus-nephritis
#6
Elkin Navarro-Quiroz, Lisandro Pacheco-Lugo, Roberto Navarro-Quiroz, Hernan Lorenzi, Pierine España-Puccini, Yirys Díaz-Olmos, Lisneth Almendrales, Valeria Olave, Henry Gonzalez-Torres, Anderson Diaz-Perez, Alex Dominguez, Antonio Iglesias, Raul García, Gustavo Aroca-Martinez
Renal involvement in Systemic Lupus Erythematous (SLE) patients is one of the leading causes of morbidity and a significant contributor to mortality. It's estimated that nearly 50% of SLE individuals develop kidney disease in the first year of the diagnosis. Class IV lupus nephritis (LN-IV) is the class of lupus nephritis most common in Colombian patients with SLE. Altered miRNAs expression levels have been reported in human autoimmune diseases including lupus. Variations in the expression pattern of peripheral blood circulating miRNAs specific for this class of lupus nephritis could be correlated with the pathophysiological status of this group of individuals...
2017: PloS One
https://www.readbyqxmd.com/read/29132874/combining-serum-microrna-and-ca-125-as-prognostic-indicators-of-preoperative-surgical-outcome-in-women-with-high-grade-serous-ovarian-cancer
#7
Jaynish S Shah, Gregory B Gard, Jean Yang, Jayne Maidens, Susan Valmadre, Patsy S Soon, Deborah J Marsh
OBJECTIVES: The most widely used approach for the clinical management of women with high-grade serous ovarian cancer (HGSOC) is surgery, followed by platinum and taxane based chemotherapy. The degree of macroscopic disease remaining at the conclusion of surgery is a key prognostic factor determining progression free and overall survival. We sought to develop a non-invasive test to assist surgeons to determine the likelihood of achieving complete surgical resection. This knowledge could be used to plan surgical approaches for optimal clinical management...
November 10, 2017: Gynecologic Oncology
https://www.readbyqxmd.com/read/29131834/plasma-microrna-profiling-exploring-better-biomarkers-for-lymphoma-surveillance
#8
Drirh Khare, Neta Goldschmidt, Aya Bardugo, Devorah Gur-Wahnon, Iddo Z Ben-Dov, Batia Avni
Early detection of relapsed lymphoma improves response and survival. Current tools lack power for detection of early relapse, while being cumbersome and expensive. We searched for sensitive biomarkers that precede clinical relapse, and serve for further studies on therapy response and relapse. We recruited 20 healthy adults, 14 diffuse large B-cell lymphoma (DLBCL) patients and 11 Hodgkin lymphoma (HL) patients at diagnosis. Using small-RNA sequencing we identified in DLBCL patients increased plasma levels of miR-124 and miR-532-5p, and decreased levels of miR-425, miR-141, miR-145, miR-197, miR-345, miR-424, miR-128 and miR-122...
2017: PloS One
https://www.readbyqxmd.com/read/29131009/effects-of-microrna-499-on-the-inflammatory-damage-of-endothelial-cells-during-coronary-artery-disease-via-the-targeting-of-pdcd4-through-the-nf-%C3%AE-%C3%AE-tnf-%C3%AE-signaling-pathway
#9
Yu-Hong Zhang, Keng He, Gang Shi
BACKGROUND/AIMS: This study aimed to analyze the impact of microRNA-499 (miR-499) on the inflammatory damage of endothelial cells during coronary artery disease (CAD) via the targeting of PDCD4 through the NF-kB/ TNF-α signaling pathway. METHODS: A total of 216 CAD patients (CAD group) and 90 healthy people (normal group) were enrolled in our study. Endothelial cells were collected and assigned into normal, OX-LDL, negative control (NC), miR-499 inhibitor, miR-499 mimic, PDCD4 siRNA, and miR-499 inhibitor + PDCD4 siRNA groups...
November 6, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29130611/usefulness-of-exosome-encapsulated-microrna-451a-as-a-minimally-invasive-biomarker-for-prediction-of-recurrence-and-prognosis-in-pancreatic-ductal-adenocarcinoma
#10
Kunihiko Takahasi, Hisae Iinuma, Keita Wada, Shunryo Minezaki, Sachiyo Kawamura, Masahiko Kainuma, Yutaka Ikede, Makoto Shibuya, Fumihiko Miura, Keiji Sano
BACKGROUND: MicroRNAs (miRNAs) encapsulated in the exosomes of plasma is of interest as stable and minimally invasive biomarkers for recurrence and prognosis in cancer patients. The aim of this study was to clarify the predictive and prognostic value of plasma exosomal microRNA-451a (miR-451a) in patients with pancreatic ductal adenocarcinoma (PDAC). METHODS: Microarray-based expression profiling of miRNAs derived from exosomes in the plasma of 6 PDAC patients with UICC stage II was employed to identify a biomarker to distinguish between patients with and without recurrence...
November 11, 2017: Journal of Hepato-biliary-pancreatic Sciences
https://www.readbyqxmd.com/read/29126951/circulating-micrornas-as-novel-biomarkers-for-heart-failure
#11
Feng Chen, Jiefu Yang, Yingying Li, Hua Wang
BACKGROUND: The diagnosis of heart failure (HF) remains difficult. A wide variety of studies have indicated that microRNAs play important roles in the pathophysiology of HF. HYPOTHESIS: Circulating miRNAs may be the potential biomarkers for HF. METHODS: In this study, a genome-wide plasma miRNA expression analysis was performed in thirteen HF patients and three controls. Microarray was used for the initial screen, followed by validation with quantitative real-time PCR (qRT-PCR) assays in an independent cohort of thirty-three HF patients and twenty controls...
November 7, 2017: Hellenic Journal of Cardiology: HJC, Hellēnikē Kardiologikē Epitheōrēsē
https://www.readbyqxmd.com/read/29125182/circulating-micrornas-as-potential-biomarkers-in-myasthenia-gravis-patients
#12
REVIEW
Anna Rostedt Punga, Tanel Punga
MicroRNAs (miRNAs) are small noncoding RNA molecules that bind to specific mRNA targets and regulate a wide range of important biological processes within cells. Circulating miRNAs are released into the extracellular space and can be measured in most biofluids, including blood serum and plasma. Recently, circulating miRNAs have emerged as easily accessible markers in various body fluids with different profiles and quantities specific for different human disorders, including autoimmune diseases. In myasthenia gravis (MG), diagnostic tests such as titers of serum autoantibodies specific for either the acetylcholine receptor (AChR(+) ) or muscle-specific tyrosine kinase (MuSK(+) ) do not necessarily reflect disease progression, and there is a great need for reliable objective biomarkers to monitor the disease course and therapeutic response...
November 10, 2017: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/29122890/microrna-30c-reduces-plasma-cholesterol-in-homozygous-familial-hypercholesterolemic-and-type-2-diabetic-mouse-models
#13
Sara Irani, Jahangir Iqbal, W James Antoni, Laraib Ijaz, M Mahmood Hussain
High plasma cholesterol levels are found in several metabolic disorders and their reductions are advocated to reduce risk of atherosclerosis. A way to lower plasma lipids is to curtail lipoprotein production; however, this is associated with steatosis. We previously showed that microRNA-30c (miR-30c) lowers diet-induced hypercholesterolemia and atherosclerosis in C57BL/6J and Apoe-/- mice. Here, we tested miR-30c effect on plasma lipids, transaminases and hepatic lipids in different mouse models. Hepatic delivery of miR-30c to chow fed leptin deficient (ob/ob) and leptin receptor deficient (db/db) hypercholesterolemic and hyperglycemic mice reduced cholesterol in total plasma and VLDL/LDL by ~28% and ~25%, respectively, without affecting triglyceride and glucose levels...
November 9, 2017: Journal of Lipid Research
https://www.readbyqxmd.com/read/29121858/detection-of-circulating-mirnas-comparative-analysis-of-extracellular-vesicle-incorporated-mirnas-and-cell-free-mirnas-in-whole-plasma-of-prostate-cancer-patients
#14
Edgars Endzeliņš, Andreas Berger, Vita Melne, Cristina Bajo-Santos, Kristīne Soboļevska, Artūrs Ābols, Marta Rodriguez, Daiga Šantare, Anastasija Rudņickiha, Vilnis Lietuvietis, Alicia Llorente, Aija Linē
BACKGROUND: Circulating cell-free miRNAs have emerged as promising minimally-invasive biomarkers for early detection, prognosis and monitoring of cancer. They can exist in the bloodstream incorporated into extracellular vesicles (EVs) and ribonucleoprotein complexes. However, it is still debated if EVs contain biologically meaningful amounts of miRNAs and may provide a better source of miRNA biomarkers than whole plasma. The aim of this study was to systematically compare the diagnostic potential of prostate cancer-associated miRNAs in whole plasma and in plasma EVs...
November 9, 2017: BMC Cancer
https://www.readbyqxmd.com/read/29119054/mir-216b-functions-as-a-tumor-suppressor-by-targeting-hmgb1-mediated-jak2-stat3-signaling-way-in-colorectal-cancer
#15
Xiaoxiang Chen, Xiangxiang Liu, Bangshun He, Yuqin Pan, Huiling Sun, Tao Xu, Xiuxiu Hu, Shukui Wang
MiR-216b is implicated in the development of multiple types of cancers, however, a role for miR-216b in colorectal cancer (CRC) remains elusive. The present study aimed to investigate the function and underlying mechanism of miR-216b in human CRC. In this study, we found miR-216b in CRC tissues and cell lines was markedly decreased compared with corresponding adjacent normal tissues (ANTs) and colonic mucosal epithelial cell line (FHC), and was obviously associated with the TNM stage, lymph node metastases, differentiation and poor overall survival (OS) (P<0...
2017: American Journal of Cancer Research
https://www.readbyqxmd.com/read/29118269/genetic-architecture-and-regulatory-impact-on-hepatic-microrna-expression-linked-to-immune-and-metabolic-traits
#16
Siriluck Ponsuksili, Nares Trakooljul, Frieder Hadlich, Fiete Haack, Eduard Murani, Klaus Wimmers
Regulation of microRNA (miRNA) expression contributes to a wide range of target gene expression and phenotypes. The miRNA expression in the liver, the central metabolic organ, was examined in 209 pigs, and integrated with haematological and clinical biomarkers of metabolic and overall health, mRNA-target expression levels and single-nucleotide polymorphism (SNP) genotypes. The expression levels of 426 miRNA species correlated with plasma haematological or biochemical traits (r² = |0.19-0.45|, false discovery rate < 5%)...
November 2017: Open Biology
https://www.readbyqxmd.com/read/29116107/micrornas-regulating-superoxide-dismutase-2-are-new-circulating-biomarkers-of-heart-failure
#17
Emilie Dubois-Deruy, Marie Cuvelliez, Jan Fiedler, Henri Charrier, Paul Mulder, Eleonore Hebbar, Angelika Pfanne, Olivia Beseme, Maggy Chwastyniak, Philippe Amouyel, Vincent Richard, Christophe Bauters, Thomas Thum, Florence Pinet
Although several risk factors such as infarct size have been identified, the progression of heart failure (HF) remains difficult to predict in clinical practice. Using an experimental rat model of post-myocardial infarction (MI), we previously identified 45 proteins differentially modulated during HF by proteomic analysis. This study sought to identify microRNAs (miRNAs) able to regulate these proteins and to test their relevance as biomarkers for HF. In silico bioinformatical analysis selected 13 miRNAs related to the 45 proteins previously identified...
November 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29115644/identification-of-differentially-expressed-micrornas-in-acute-kawasaki-disease
#18
Ye Chen, Yue-Yue Ding, Yan Ren, Lei Cao, Qiu-Qin Xu, Ling Sun, Ming-Guo Xu, Hai-Tao Lv
The present study used microarray analysis to screen the plasma expression of microRNAs (miRNAs) in patients with acute Kawasaki disease (KD) and aimed to explore the pathogenesis of KD. Plasma was collected from children with acute KD (n=6) and from healthy control children (n=6). Total RNA was extracted and differential miRNA expression between the two groups was determined. Differentially expressed miRNAs were validated using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) in an independent cohort (n=8)...
November 8, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29114076/circular-rna-dlgap4-ameliorates-ischemic-stroke-outcomes-by-targeting-mir-143-to-regulate-endothelial-mesenchymal-transition-associated-with-blood-brain-barrier-integrity
#19
Ying Bai, Yuan Zhang, Bing Han, Li Yang, Xufeng Chen, Rongrong Huang, Fangfang Wu, Jie Chao, Pei Liu, Gang Hu, John H Zhang, Honghong Yao
Circular RNAs (circRNAs) are highly expressed in the central nervous system and regulate physiological and pathophysiological processes. However, the potential role of circRNAs in stroke remains largely unknown. Here, we show that the circular RNA DLGAP4 (circDLGAP4) functions as an endogenous microRNA-143 (miR-143) sponge to inhibit miR-143 activity, resulting in the inhibition of HECT domain E3 ubiquitin protein ligase 1 (HECTD1) expression. CircDLGAP4 levels were significantly decreased in the plasma of acute ischemic stroke patients (13 females and 13 males) and in a mouse stroke model...
November 7, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/29113501/fabrication-of-niclosamide-loaded-solid-lipid-nanoparticles-in-vitro-characterization-and-comparative-in-vivo-evaluation
#20
Maqsood Ur Rehman, Mir Azam Khan, Waheed S Khan, Muhammad Shafique, Munasib Khan
Niclosamide (NCS) is an oral anthelminthic drug having low solubility and hence low bioavailability. Current investigation shows an approach to fabricate solid lipid nanoparticles (SLNs) of NCS and evaluated for pharmaceutical, in vitro and in vivo characterization. NFM-3 showed particle size 204.2 ± 2.2 nm, polydispersity index 0.328 ± 0.02 and zeta potential -33.16 ± 2 mV. Entrapment efficiency and drug loading capacity were 84.4 ± 0.02% and 5.27 ± 0.03%, respectively. Scanning electron microscopy image indicated that particles were nanoranged...
November 7, 2017: Artificial Cells, Nanomedicine, and Biotechnology
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