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staphylococcus epidermidis multiresistant

Wafi Siala, Hector Rodriguez-Villalobos, Prabhavathi Fernandes, Paul M Tulkens, Françoise Van Bambeke
Staphylococcal biofilms are a major cause of therapeutic failure, especially when caused by multiresistant strains. Oral fusidic acid is currently being re-developed in the US for skin and skin structure and orthopedic infections, in which biofilms play a major role. The aim of this study was to examine the activity of fusidic acid alone or combined with other anti-staphylococcal drugs against biofilms made by a reference strain and five clinical isolates of Staphylococcus aureus or Staphylococcus epidermidis in in vitro static or dynamic models (microtiter plates and CDC reactor) exposed to clinically-relevant concentrations...
April 30, 2018: Antimicrobial Agents and Chemotherapy
Alexandros Lazaris, David C Coleman, Angela M Kearns, Bruno Pichon, Peter M Kinnevey, Megan R Earls, Breida Boyle, Brian O'Connell, Gráinne I Brennan, Anna C Shore
Background: Linezolid is often the drug of last resort to treat infections caused by Gram-positive cocci. Linezolid resistance can be mutational (23S rRNA or L-protein) or, less commonly, acquired [predominantly cfr, conferring resistance to phenicols, lincosamides, oxazolidinones, pleuromutilins and streptogramin A compounds (PhLOPSA) or optrA, encoding oxazolidinone and phenicol resistance]. Objectives: To investigate the clonality and genetic basis of linezolid resistance in 13 linezolid-resistant (LZDR) methicillin-resistant Staphylococcus epidermidis (MRSE) isolates recovered during a 2013/14 outbreak in an ICU in an Irish hospital and an LZDR vancomycin-resistant Enterococcus faecium (VRE) isolate from an LZDR-MRSE-positive patient...
December 1, 2017: Journal of Antimicrobial Chemotherapy
Marjory Xavier Rodrigues, Nathália Cristina Cirone Silva, Júlia Hellmeister Trevilin, Melina Mary Bravo Cruzado, Tsai Siu Mui, Fábio Rodrigo Sanches Duarte, Carmen J Contreras Castillo, Solange Guidolin Canniatti-Brazaca, Ernani Porto
The aim of this research paper was to characterize coagulase-positive and coagulase-negative staphylococci from raw milk, Minas cheese, and production lines of Minas cheese processing. One hundred isolates from 3 different cheese producers were characterized using molecular approaches, such as PCR, molecular typing, and DNA sequencing. Staphylococcus aureus (88% of the isolates) was the most abundant followed by Staphylococcus epidermidis, Staphylococcus hyicus, and Staphylococcus warneri. Among the 22 enterotoxin genes tested, the most frequent was seh (62% of the isolates), followed by selx and ser...
July 2017: Journal of Dairy Science
I Klimiene, M Virgailis, A Pavilonis, R Siugzdiniene, R Mockeliunas, M Ruzauskas
The objectives of this study were to determine the prevalence and antimicrobial resistance of coagulase-negative staphylococci (CNS) isolated from dairy cows with subclinical mastitis. Antimicrobial resistance in staphylococci were evaluated by breakpoint values specific to the species (EU-CAST). The presence of resistance-encoding genes was detected by multiplex PCR. A total of 191 CNS isolates were obtained. The CNS isolates were typically resistant to penicillin (67.4%), tetracyc-line (18.9%), and erythromycin (13...
September 1, 2016: Polish Journal of Veterinary Sciences
Andrea Brenciani, Gianluca Morroni, Marina Mingoia, Pietro E Varaldo, Eleonora Giovanetti
This study investigated the stability or instability - i.e. the ability or inability to undergo excision in circular form - of the four cargo regions (cr1 to cr4) of the novel cfr-carrying, multiresistance plasmid pSP01, arboured by a clinical Staphylococcus epidermidis isolate. Only cr4 proved unstable. The stability of cr1 and cr2 was substantially expected. Insertion sequences (ISs) played an important role in the stability of cr3 (the cfr gene context) and in the instability of cr4. Whereas the stability of cfr genetic contexts is associated with the presence of a single IS copy (istAS-istBS in cr3), their instability is associated with two identical, flanking ISs with the same orientation...
December 2016: International Journal of Medical Microbiology: IJMM
Héctor Daniel Salazar-Holguín, María Elena Cisneros-Robledo
BACKGROUND: The antibibiotic resistance by nosocomial infections (NI) causal agents constitutes a seriously global problematic that involves the Mexican Institute of Social Security's Regional General Hospital 1 in Chihuahua, Mexico; although with special features that required to be specified and evaluated, in order to concrete an effective therapy. METHODS: Observational, descriptive and prospective study; by means of active vigilance all along 2014 in order to detect the nosocomial infections, for epidemiologic study, culture and antibiogram to identify its causal agents and antibiotics resistance and sensitivity...
July 2016: Revista Médica del Instituto Mexicano del Seguro Social
Ariane Deplano, Stien Vandendriessche, Claire Nonhoff, Magali Dodémont, Sandrine Roisin, Olivier Denis
OBJECTIVES: The objectives of this study were: (i) to determine the species diversity of CoNS isolated from bloodstream infections collected during a national surveillance study; and (ii) to examine the antimicrobial resistance and genomic diversity among Staphylococcus epidermidis isolates. METHODS: Eighty CoNS were identified by MALDI-TOF. Antimicrobial resistance determination, molecular characterization of resistance and virulence genes, and molecular typing were performed for S...
July 2016: Journal of Antimicrobial Chemotherapy
Christiane Cuny, Phillippe Arnold, Julia Hermes, Tim Eckmanns, Jaishri Mehraj, Sonja Schoenfelder, Wilma Ziebuhr, Qin Zhao, Yang Wang, Andrea T Feßler, Gérard Krause, Stefan Schwarz, Wolfgang Witte
This study reports on the emergence of linezolid-resistant coagulase-negative staphylococci (CoNS) containing the multiresistance gene cfr in veal calves and pigs, as well as in humans exposed to these animals. CoNS (Staphylococcus auricularis, Staphylococcus cohnii, Staphylococcus lentus, Staphylococcus kloosii, Staphylococcus sciuri, Staphylococcus simulans), but not Staphylococcus aureus, carrying the gene cfr were detected in samples of 12 out of 52 calves at three farms which had a history of florfenicol use...
February 2017: Veterinary Microbiology
Urszula Posadowska, Monika Brzychczy-Włoch, Elżbieta Pamuła
Since there are more and more cases of multiresistance among microorganisms, rational use of antibiotics (especially their systemic vs. local application) is of great importance. Here we propose polymeric nanoparticles as locally applied gentamicin delivery system useful in osteomyelitis therapy. Gentamicin sulphate (GS) was encapsulated in the poly(lactide-co-glycolide) (PLGA 85:15) nanoparticles by double emulsification (water/oil/water, W1/O/W2). The nanoparticles were characterized by dynamic light scattering, laser electrophoresis and atomic force microscopy...
2015: Acta of Bioengineering and Biomechanics
D Zajonz, L Wuthe, A C Rodloff, T Prietzel, G Freiherr von Salis-Soglio, A Roth, C-E Heyde, C Josten, M Ghanem
BACKGROUND: Because of the rise in primary implantations in elective knee and hip arthroplasty, the number of complications, particularly due to prosthetic infections has increased. Partly due to multimorbidities, an increase in geriatric patients and often unnecessary use of antibiotics, a change in the spectrum of bacteria with an increase in multi-drug resistant pathogens is to be expected. For physicians this creates not only new medical and economic but also sociopolitical challenges...
April 2016: Der Chirurg; Zeitschrift Für Alle Gebiete der Operativen Medizen
Fabio Cafini, Le Thuy Thi Nguyen, Masato Higashide, Federico Román, José Prieto, Kazuya Morikawa
OBJECTIVES: Linezolid resistance mediated by the cfr gene represents a global concern due to its dissemination among multiresistant nosocomial pathogens such as MRSA and Enterococcus. In the present work, we have evaluated the in vitro transmission of cfr pSCFS7-like plasmids from two Staphylococcus epidermidis ST2 strains (SE45 and SE50) isolated in Spanish hospitals, to clinical MRSA and Enterococcus spp. isolates obtained in Japan, a country in which cfr has not been detected yet. We have also investigated alternative mechanisms of horizontal gene transfer involved in the spread of the cfr gene...
March 2016: Journal of Antimicrobial Chemotherapy
Andrea Brenciani, Gianluca Morroni, Simona Pollini, Erika Tiberi, Marina Mingoia, Pietro E Varaldo, Gian Maria Rossolini, Eleonora Giovanetti
OBJECTIVES: The objective of this study was to investigate the genetic environment of the cfr gene from two linezolid-resistant clinical isolates of Staphylococcus epidermidis from Italy. METHODS: The two strains (SP1 and SP2) were phenotypically and genotypically characterized. Transferability of cfr was assessed by electrotransformation and conjugation. The genetic contexts of cfr were investigated by PCR mapping, sequencing and comparative sequence analyses. RESULTS: SP1 and SP2 belonged to ST23 and ST83, respectively...
February 2016: Journal of Antimicrobial Chemotherapy
Monica Bortolin, Elena De Vecchi, Carlo Luca Romanò, Marco Toscano, Roberto Mattina, Lorenzo Drago
OBJECTIVES: The treatment of bone and joint infections is challenging due to the presence of bacterial biofilm and the increasing emergence of multiresistant strains. BAG-S53P4 is a bone substitute that is characterized by osteoconductive and antimicrobial properties. The aim of this study was to assess the effectiveness of BAG-S53P4 against biofilm produced in vitro by multiresistant bacterial strains. METHODS: Multiresistant Staphylococcus epidermidis, Acinetobacter baumannii and Klebsiella pneumoniae isolated from bone and joint infections were used in this study...
January 2016: Journal of Antimicrobial Chemotherapy
Jean-Winoc Decousser, Marine Desroches, Nadège Bourgeois-Nicolaos, Julien Potier, François Jehl, Gérard Lina, Vincent Cattoir, François Vandenesh, Florence Doucet-Populaire
Multiresistance in staphylococci constitutes a major challenge for the antimicrobial chemotherapy of invasive infections such as bacteraemia or bone and joint infections (BJIs). A nationwide prospective study was performed to detect antimicrobial resistance trends among staphylococci causing invasive infections. Between October 2011 and February 2012, 367 meticillin-resistant Staphylococcus aureus (MRSA) and 695 coagulase-negative staphylococci (CoNS) were collected from 37 French hospitals, mainly from bacteraemia (59...
December 2015: International Journal of Antimicrobial Agents
Tomasz Czekaj, Marcin Ciszewski, Eligia M Szewczyk
Staphylococcus haemolyticus is one of the most frequent aetiological factors of staphylococcal infections. This species seems to lack the important virulence attributes described in other staphylococci. However, studies have shown that the presence of various enzymes, cytolysins and surface substances affects the virulence of S. haemolyticus. Nevertheless, none of them has been identified as crucial and determinative. Despite this, S. haemolyticus is, after Staphylococcus epidermidis, the second most frequently isolated coagulase-negative staphylococcus from clinical cases, notably from blood infections, including sepsis...
November 2015: Microbiology
Juan Manuel Bello-López, Jorge Noguerón-Silva, Jorge Ismael Castañeda-Sánchez, Julieta Rojo-Medina
Disposal of Umbilical Cord Blood Units due to microbial contamination is a major problem in Cord Blood Banks worldwide as it reduces the number of units available for transplantation. Additionally, economic losses are generated as result of resources and infrastructure used to obtain such units. Umbilical Cord Blood Units that showed initial microbial contamination were subject to strains isolation, identification, and characterization by sequencing the 16S rRNA gene and Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR)...
November 2015: Brazilian Journal of Infectious Diseases
M Butin, P Martins-Simões, J C Picaud, A Kearns, O Claris, F Vandenesch, F Laurent, J P Rasigade
OBJECTIVES: The Staphylococcus capitis clone NRCS-A has recently been described as a frequent cause of late-onset sepsis (LOS) in pre-term neonates worldwide. Representatives of this clone exhibit non-susceptibility to vancomycin, the first-line agent used in LOS. Cases of prolonged S. capitis LOS despite vancomycin treatment have been reported. We investigated whether NRCS-A strains exhibit faster adaptation to vancomycin pressure as compared with other staphylococci. METHODS: Strains of S...
November 2015: Journal of Antimicrobial Chemotherapy
Guillaume Villatte, Christophe Massard, Stéphane Descamps, Yves Sibaud, Christiane Forestier, Komla-Oscar Awitor
External fixation is a method of osteosynthesis currently used in traumatology and orthopedic surgery. Pin tract infection is a common problem in clinical practice. Infection occurs after bacterial colonization of the pin due to its contact with skin and the local environment. One way to prevent such local contamination is to create a specific coating that could be applied in the medical field. In this work, we developed a surface coating for external fixator pins based on the photocatalytic properties of titanium dioxide, producing a bactericidal effect with sufficient mechanical strength to be compatible with surgical use...
2015: International Journal of Nanomedicine
Sarah Wendlandt, Kristina Kadlec, Andrea T Feßler, Stefan Schwarz
The aim of this study was to investigate the genetic basis of combined pleuromutilin-lincosamide-streptogramin A resistance in 26 unrelated methicillin-resistant Staphylococcus aureus (MRSA) and coagulase-negative staphylococci (CoNS) from dairy cows suffering from mastitis. The 26 pleuromutilin-resistant staphylococcal isolates were screened for the presence of the genes vga(A), vga(B), vga(C), vga(E), vga(E) variant, sal(A), vmlR, cfr, lsa(A), lsa(B), lsa(C), and lsa(E) by PCR. None of the 26 isolates carried the genes vga(B), vga(C), vga(E), vga(E) variant, vmlR, cfr, lsa(A), lsa(B), or lsa(C)...
June 12, 2015: Veterinary Microbiology
Luís Fernando Leandro, Miguel Jorge Oliveira Cardoso, Sandro Donizeti Caetano Silva, Maria Gorete Mendes Souza, Rodrigo Cassio Sola Veneziani, Sergio Ricardo Ambrosio, Carlos Henrique Gomes Martins
Antibiotic-resistant bacteria have emerged from the widespread use of antibiotics worldwide and have prompted the search for new sources of antimicrobial substances. Pinus spp. contain several bioactive compounds consisting mainly of terpenes, terpenoids and some other aromatic and aliphatic constituents. These compounds exert important biological effects, and pine oils have found wide application in the industry. In the present study, we have evaluated the potential activity of the resin-oil of Pinus elliottii and its major compound dehydroabietic acid (DA) against multiresistant bacteria by MIC, minimum bactericidal concentration and time-kill assays...
December 2014: Journal of Medical Microbiology
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