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https://www.readbyqxmd.com/read/29924746/exercise-training-mitigates-multi-system-deconditioning-during-bed-rest
#1
Lori L Ploutz-Snyder, Meghan Downs, Elizabeth Goetchius, Brent Crowell, Kirk L English, Robert Ploutz-Snyder, Jeffrey Ryder, E Lichar Dillon, Melinda Sheffield-Moore, Jessica M Scott
INTRODUCTION: This study investigated the safety and effectiveness of a new integrated aerobic and resistance exercise training prescription (SPRINT) using two different sets of exercise equipment: a suite of large ISS-like exercise equipment similar to what is found on the International Space Station (ISS) and a single device with aerobic and resistance exercise capability in the spaceflight analog of bed rest (BR). METHODS: Subjects (n=34) completed 70 days of 6° head down tilt BR: 9 were randomized to remain sedentary (CONT), 9 to exercise training using traditional equipment (EX), 8 to exercise using traditional equipment and low dose testosterone supplementation (ExT), and 8 to exercise using a combined resistance and aerobic flywheel device (FLY)...
March 26, 2018: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/29924728/bnip3-contributes-to-the-glutamine-driven-aggressive-behavior-of-melanoma-cells
#2
Monica Vara-Perez, Hannelore Maes, Sarah Van Dingenen, Patrizia Agostinis
Aerobic glycolysis (Warburg effect) is used by cancer cells to fuel tumor growth. Interestingly, metastatic melanoma cells rely on glutaminolysis rather than aerobic glycolysis for their bioenergetic needs through the tricarboxylic acid cycle. Here, we compared the effects of glucose or glutamine on melanoma cell proliferation, migration and oxidative phosphorylation in vitro. We found that glutamine-driven melanoma cell's aggressive traits positively correlated with increased expression of HIF1α and its pro-autophagic target BNIP3...
June 1, 2018: Biological Chemistry
https://www.readbyqxmd.com/read/29923910/hypoxic-training-improves-normoxic-glucose-tolerance-in-adolescents-with-obesity
#3
Estelle De Groote, Florian A Britto, Loïc Bullock, Marie François, Carine De Buck, Henri Nielens, Louise Deldicque
PURPOSE: To test whether environmental hypoxia could potentiate the effects of exercise training on glucose metabolism and insulin sensitivity. METHODS: Fourteen adolescents with obesity were assigned to 6 weeks of exercise training either in normoxic or in hypoxic conditions (FiO2 15%). Adolescents trained 3x/w for 50-60min, including endurance and resistance exercises. Oral glucose tolerance test (OGTT), blood and morphological analyses as well as physical performance tests were performed before and after the training period...
June 20, 2018: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/29923816/ahniella-affigens-gen-nov-sp-nov-a-gammaproteobacterium-isolated-from-sandy-soil-near-a-stream
#4
Woon Mo Hwang, Yongseok Ko, Jae-Heon Kim, Keunsoo Kang
A bacterial strain, designated D13T , was isolated from sandy soil near a stream in Sinan-gun, Republic of Korea. Cells were Gram-stain-negative, aerobic, non-motile and flexible rod-shaped. Growth occurred at 15-35 °C (optimum 30 °C) and pH 6.5-8.0 (pH 7.0). NaCl was not obligatory for growth but could be tolerated at up to 0.5 % (w/v) NaCl. The DNA G+C content of the genomic DNA of strain D13T was 57.7 mol% and a phylogenetic analysis of the 16S rRNA gene sequence revealed that strain D13T formed a distinct evolutionary lineage within the family Rhodanobacteraceae of the order Lysobacterales...
June 20, 2018: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29923623/aerobic-physical-activity-assessed-with-accelerometer-diary-questionnaire-and-interview-in-a-finnish-population-sample
#5
Henna Hukkanen, Pauliina Husu, Harri Sievänen, Kari Tokola, Henri Vähä-Ypyä, Heli Valkeinen, Tomi Mäki-Opas, Jaana H Suni, Tommi Vasankari
This study evaluates the agreement between different methods to assess moderate to vigorous physical activity (MVPA) in a large sample of Finnish adults. Methods were classified and examined pairwise (accelerometer vs diary; questionnaire vs interview). Proportion of participants meeting the aerobic health-enhancing physical activity (HEPA) recommendation was compared pairwise between all four methods. The present study of 1916 adults aged 18-75 years (mean age 50 yrs., 57% women) is a sub-sample of population-based Health 2011 Study conducted by the National Institute of Health and Welfare in Finland...
June 20, 2018: Scandinavian Journal of Medicine & Science in Sports
https://www.readbyqxmd.com/read/29923108/assessing-cardiorespiratory-fitness-of-soccer-players-is-test-specificity-the-issue-a-review
#6
Monèm Jemni, Mohammad Shoaib Prince, Julien S Baker
It is important that players and coaches have access to objective information on soccer player's physical status for team selection and training purposes. Physiological tests can provide this information. Physiological testing in laboratories and field settings are very common, but both methods have been questioned because of their specificity and accuracy respectively. Currently, football players have their direct aerobic fitness assessed in laboratories using treadmills or cycle ergometers, whilst indirect measures (using estimation of aerobic performance) are performed in the field, typically comprising multiple shuttle runs back and forth over a set distance...
June 19, 2018: Sports Medicine—Open
https://www.readbyqxmd.com/read/29922706/soy-protein-improves-tibial-whole-bone-and-tissue-level-biomechanical-properties-in-ovariectomized-and-ovary-intact-low-fit-female-rats
#7
Pamela S Hinton, Laura C Ortinau, Rebecca K Dirkes, Emily L Shaw, Matthew W Richard, Terese Z Zidon, Steven L Britton, Lauren G Koch, Victoria J Vieira-Potter
Background: Osteoporosis and related fractures, decreased physical activity, and metabolic dysfunction are serious health concerns for postmenopausal women. Soy protein might counter the negative effects of menopause on bone and metabolic health due to the additive or synergistic effects of its bioactive components. Objective: To evaluate the effects of ovariectomy (OVX) and a soy-protein diet (SOY) on bone outcomes in female, low-capacity running (LCR) rats selectively bred for low aerobic fitness as a model of menopause...
June 2018: Bone Reports
https://www.readbyqxmd.com/read/29922392/correlation-between-match-performance-and-field-tests-in-professional-soccer-players
#8
Paulo E Redkva, Mauro R Paes, Ricardo Fernandez, Sergio G da-Silva
The aim of this study was to investigate possible correlations between aerobic and anaerobic fitness (in field tests) with performance during a 90 min friendly match, through the following variables: total distance covered, maximal running speed, number of high-intensity actions and number of sprints. Eighteen professional soccer players from a Brazilian elite team (age 23 ± 3 years, body mass 77.5 ± 8.9 kg) participated in the study. The athletes performed a Yo-Yo Endurance Test (aerobic fitness) and a Running Anaerobic Sprint Test (six maximal 35 m efforts separated by 10 s of passive recovery, anaerobic fitness)...
June 2018: Journal of Human Kinetics
https://www.readbyqxmd.com/read/29922184/standardized-assessment-of-resistance-training-induced-subjective-symptoms-and-objective-signs-of-immunological-stress-responses-in-young-athletes
#9
Christian Puta, Thomas Steidten, Philipp Baumbach, Toni Wöhrl, Rico May, Michael Kellmann, Marco Herbsleb, Brunhild Gabriel, Stephanie Weber, Urs Granacher, Holger H W Gabriel
From a health and performance-related perspective, it is crucial to evaluate subjective symptoms and objective signs of acute training-induced immunological responses in young athletes. The limited number of available studies focused on immunological adaptations following aerobic training. Hardly any studies have been conducted on resistance-training induced stress responses. Therefore, the aim of this observational study was to investigate subjective symptoms and objective signs of immunological stress responses following resistance training in young athletes...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29921665/four-molybdenum-dependent-steroid-c-25-hydroxylases-heterologous-overproduction-role-in-steroid-degradation-and-application-for-25-hydroxyvitamin-d-3-synthesis
#10
Christian Jacoby, Jens Eipper, Markus Warnke, Oliver Tiedt, Mario Mergelsberg, Hans-Joachim Stärk, Birgit Daus, Zaira Martín-Moldes, María Teresa Zamarro, Eduardo Díaz, Matthias Boll
Side chain-containing steroids are ubiquitous constituents of biological membranes that are persistent to biodegradation. Aerobic, steroid-degrading bacteria employ oxygenases for isoprenoid side chain and tetracyclic steran ring cleavage. In contrast, a Mo-containing steroid C-25 dehydrogenase (S25DH) of the dimethyl sulfoxide (DMSO) reductase family catalyzes the oxygen-independent hydroxylation of tertiary C-25 in the anaerobic, cholesterol-degrading bacterium Sterolibacterium denitrificans Its genome contains eight paralogous genes encoding active site α-subunits of putative S25DH-like proteins...
June 19, 2018: MBio
https://www.readbyqxmd.com/read/29921654/muscular-weakness-in-adolescence-is-associated-with-disability-30-years-later-a-population-based-cohort-study-of-1-2-million-men
#11
Hanna Henriksson, Pontus Henriksson, Per Tynelius, Francisco B Ortega
OBJECTIVE: To investigate the associations of muscular strength in adolescence with later disability pension (DP), across different body mass index (BMI) categories and in combination with aerobic fitness. METHOD: This prospective cohort study consisted of males aged 16-19 years, recruited from the Swedish military conscription register between 1969 and 1994. A total of 1 212 503 adolescents met all the inclusion criteria and were therefore included in the analyses...
June 19, 2018: British Journal of Sports Medicine
https://www.readbyqxmd.com/read/29921394/saccharomyces-cerevisiae-key-role-of-mig1-gene-in-metabolic-switching-putative-fermentation-oxidation
#12
I Alipourfard, S Bakhtiyari, N Datukishvili, K Haghani, L Di Renzo, R C De Miranda, G Cioccoloni, P Basiratyan Yazdi, D Mikeladze
Saccharomyces cerevisiae can utilize a wide range of carbon sources; however, in the presence of glucose the use of alternate carbon sources would be repressed. Several genes involved in the metabolic pathways exert these effects. Among them, the zinc finger protein, Mig1 (multicopy inhibitor of GAL gene expression) plays important roles in glucose repression of Saccharomyces cerevisiae. To investigate whether the alleviation of glucose effect would result in a switch to oxidative production pathway, MIG1 were disrupted in a haploid laboratory strain (2805) of S...
May 2018: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/29921005/microbial-life-under-ice-metagenome-diversity-and-in-situ-activity-of-verrucomicrobia-in-seasonally-ice-covered-lakes
#13
Patricia Tran, Arthi Ramachandran, Ola Khawasek, Beatrix E Beisner, Milla Rautio, Yannick Huot, David A Walsh
Northern lakes are ice-covered for a large part of the year, yet our understanding of microbial diversity and activity during winter lags behind that of the ice-free period. In this study, we investigated under-ice diversity and metabolism of Verrucomicrobia in seasonally ice-covered lakes in temperate and boreal regions of Quebec, Canada using 16S rRNA sequencing, metagenomics and metatranscriptomics. Verrucomicrobia, particularly the V1, V3 and V4 subdivisions, were abundant during ice-covered periods. A diversity of Verrucomicrobia genomes were reconstructed from Quebec lake metagenomes...
June 19, 2018: Environmental Microbiology
https://www.readbyqxmd.com/read/29920461/multi-omics-comparison-reveals-metabolome-biochemistry-not-microbiome-composition-or-gene-expression-corresponds-to-elevated-biogeochemical-function-in-the-hyporheic-zone
#14
Emily B Graham, Alex R Crump, David W Kennedy, Evan Arntzen, Sarah Fansler, Samuel O Purvine, Carrie D Nicora, William Nelson, Malak M Tfaily, James C Stegen
Biogeochemical hotspots are pervasive at terrestrial-aquatic interfaces, particularly within groundwater-surface water mixing zones (hyporheic zones), and they are critical to understanding spatiotemporal variation in biogeochemical cycling. Here, we use multi 'omic comparisons of hotspots to low-activity sediments to gain mechanistic insight into hyporheic zone organic matter processing. We hypothesized that microbiome structure and function, as described by metagenomics and metaproteomics, would distinguish hotspots from low-activity sediments by shifting metabolism towards carbohydrate-utilizing pathways and elucidate discrete mechanisms governing organic matter processing in each location...
June 16, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29920445/highly-efficient-of-nitrogen-removal-from-mature-landfill-leachate-using-a-combined-dn-pn-anammox-process-with-a-dual-recycling-system
#15
Xiang Li, Yan Yuan, Fan Wang, Yong Huang, Qing-Tan Qiu, Yuan Yi, Zhen Bi
An efficient and stable combined denitrification-partial nitrification-Anammox process with a dual recycling system was used to remove nitrogen from mature landfill leachate. After 155 d of operation, the NO3 - as the PN-Anammox byproduct was almost treated with biodegradable organic carbon in raw wastewater in a pre-denitrification reactor by external recycling system. When raw landfill leachate with NH4 + -N concentration of 1900 mg/L was treated, an integrated reactor with airlift recycling was combined with the PN and Anammox reactions to efficiently remove NH4 + from the inflow...
June 9, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29920232/assessing-ventilatory-threshold-in-individuals-with-motor-complete-spinal-cord-injury
#16
Jason S Au, Arjun Sithamparapillai, Katharine D Currie, Andrei V Krassioukov, Maureen J MacDonald, Audrey L Hicks
OBJECTIVE: To assess the feasibility of measuring ventilatory threshold (VT) in higher-level, motor-complete spinal cord injury (SCI) using four different analysis methods based on non-invasive gas exchange. DESIGN: Observational. SETTING: Laboratory testing. PARTICIPANTS: Individuals with C4-T6 motor-complete SCI (16 paraplegia, 22 tetraplegia; American Spinal Injury Association Impairment Scale A/B; 42±10 years of age)...
June 16, 2018: Archives of Physical Medicine and Rehabilitation
https://www.readbyqxmd.com/read/29917330/microbial-methylotrophic-metabolism-recent-metabolic-modeling-efforts-and-their-applications-in-industrial-biotechnology
#17
REVIEW
Christian Lieven, Markus J Herrgård, Nikolaus Sonnenschein
Developing methylotrophic bacteria into cell factories that meet the chemical demand of the future could be both economical and environmentally friendly. Methane is not only an abundant, low-cost resource but also a potent greenhouse gas, the capture of which could help to reduce greenhouse gas emissions. Rational strain design workflows rely on the availability of carefully combined knowledge often in the form of genome-scale metabolic models to construct high-producer organisms. In this review, we present the most recent genome-scale metabolic models in aerobic methylotrophy and their applications...
June 19, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/29916971/cervicofacial-necrotizing-fasciitis-and-drugs
#18
Laís Sara Egas, Erik Neiva Ribeiro de Carvalho Reis, Leonardo Freitas da Silva, João Paulo Bonardi, Valthierre Nunes de Lima, Pedro Henrique Silva Gomes Ferreira, Ciro Borges Duailibe de Deus, Idelmo Rangel Garcia Júnior
Necrotizing fasciitis (NF) is a devastating disease that typically affects immunocompromised patients, chronically debilitated patients or drug users, but can also affect healthy patients. Necrotizing fasciitis can rapidly produce septic shock and requires immediate surgical management of the necrotic tissue. It is a bacterial infection that progresses rapidly and has a high mortality generally caused by aerobic and anaerobic bacteria. The patient was immunocompromised and drug user. During treatment, a combination of broad-spectrum antibiotic therapy with Ciprofloxacin and Metronidazole, besides the use of activated charcoal dressing composed of carbonized fabric and impregnated with 0...
June 18, 2018: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/29916795/roseomonas-radiodurans-sp-nov-a-gamma-radiation-resistant-bacterium-isolated-from-gamma-ray-irradiated-soil
#19
Ju-Young Kim, Dong-Uk Kim, Myung-Suk Kang, Jun Hwee Jang, Su Jeong Kim, Min Ji Kim, Ju Yeon Lee, Yoon Seo Lee, Jing Zhang, Sangyong Lim, Myung Kyum Kim
A bacterial strain, designated 17Sr1-1T , was isolated from gamma ray-irradiated soil. Cells of this strain were Gram-stain-negative, strictly aerobic, motile and non-spore-forming rods. Growth occurred at 18-42 ˚C and pH 6.0-8.0, but no growth occurred at 2 % NaCl concentration. The major fatty acids of strain 17Sr1-1T were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), iso-C17 : 1ω5c and C16 : 0. The polar lipid profile contained diphosphatidylglycerol, glycolipid, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and four unidentified lipids...
June 19, 2018: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29916308/the-mtor-pathway-regulates-pkm2-to-affect-glycolysis-in-esophageal-squamous-cell-carcinoma
#20
He Xiaoyu, Yin Yiru, Shi Shuisheng, Cheng Keyan, Yan Zixing, Cheng Shanglin, Wang Yuan, Cheng Dongming, Zhang Wangliang, Bai Xudong, Ma Jie
OBJECTIVES: Esophageal squamous cell carcinoma is a highly prevalent cancer withpoor survival rate and prognosis. Increasing evidence suggests an important role for metabolic regulation in treating esophageal squamous cell carcinoma, but the underlying mechanism remains unclear. The pyruvate kinase M2 isoform is a key enzyme in the energy production process, and the upregulation of pyruvate kinase M2 isoform also plays a crucial role in gene transcription and tumorigenesis. The mammalian target of rapamycin pathway regulates an array of cellular functions, including protein synthesis, metabolism, and cell proliferation...
January 1, 2018: Technology in Cancer Research & Treatment
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