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Multiple chemical sensitivity

Jia-Kai Zeng, Yuan-Yuan Li, Tian-Ming Wang, Jie Zhong, Jia-Sheng Wu, Ping Liu, Hua Zhang, Yue-Ming Ma
A rapid, sensitive, and accurate UPLC-MS/MS method was developed for the simultaneous quantification of components of Huangqi decoction (HQD), such as calycosin-7-O-β-D-glucoside, calycosin-glucuronide, liquiritin, formononetin-glucuronide, isoliquiritin, liquiritigenin, ononin, calycosin, isoliquiritigenin, formononetin, glycyrrhizic acid, astragaloside IV, cycloastragenol, and glycyrrhetinic acid, in rat plasma. After plasma samples were extracted by protein precipitation, chromatographic separation was performed with a C18 column, using a gradient of methanol and 0...
December 15, 2017: Biomedical Chromatography: BMC
Jake Pawlowski, Ian Carrick, Igor A Kaltashov
Profiling of complex proteins by means of mass spectrometry (MS) frequently requires that certain chemical modifications of their covalent structure (e.g., reduction of disulfide bonds) be carried out prior to the MS or MS/MS analysis. Traditionally, these chemical reactions take place in the off-line mode to allow the excess reagents (the majority of which interfere with the MS measurements and degrade the analytical signal) to be removed from the protein solution prior to MS measurements. In addition to a significant increase in the analysis time, chemical reactions may result in a partial or full loss of the protein if the modifications adversely affect its stability, e...
December 14, 2017: Analytical Chemistry
Sean Yu, Pranav Joshi, Yi Ju Park, Kyeong-Nam Yu, Moo-Yeal Lee
Layer-by-layer cell printing is useful in mimicking layered tissue structures inside the human body and has great potential for being a promising tool in the field of tissue engineering, regenerative medicine, and drug discovery. However, imaging human cells cultured in multiple hydrogel layers in 3D-printed tissue constructs is challenging as the cells are not in a single focal plane. Although confocal microscopy could be a potential solution for this issue, it compromises the throughput which is a key factor in rapidly screening drug efficacy and toxicity in pharmaceutical industries...
December 14, 2017: Biotechnology Progress
Kathryn Lee, Markus Lankers, Oliver Valet
Raw materials need to be of a certain quality with respect to physical and chemical composition. They also need to have no contaminants, including particles, because these could indicate raw material impurities or contaminate the product. Particle identification allows determination of process conditions that caused them and whether the quality of the final product is acceptable. Particles may appear to the eye to be very different things than they actually are. They may be coated with the raw material and may consist of several components; therefore, chemical and elemental analyses are required for accuracy in proper identification and definitive information about their source...
January 1, 2017: Applied Spectroscopy
Ludi Wang, Wei Yang, Siyang Wu, Shuyao Wang, Chen Kang, Xiaoli Ma, Yingfei Li, Chuan Li
Isochamaejasmin, neochamaejasmin A, and daphnoretin derived from Stellera chamaejasme L. are important because of their reported anti-cancer properties. In this study, a sensitive UPLC-MS/MS method for the determination of isochamaejasmin, neochamaejasmin A, and daphnoretin in rat plasma was developed. The analyte and IS were separated on an Acquity UPLC HSS T3 column (100 × 2.1 mm, 1.8μm) using gradient elution with the mobile phase of aqueous solution (methanol/water, 1:99, v/v, containing 1 mM formic acid) and organic solution (methanol/water, 99:1, v/v, containing 1 mM formic acid) at a flow rate of 0...
December 12, 2017: Biomedical Chromatography: BMC
A Kotnala, S Senthilkumari, N Halder, A Kumar, T Velpandian
PURPOSE: To develop a microwave assisted method for the rapid synthesis of A2E and also to develop a method to quantify N-retinylidene-N-retinylethanolamine(A2E), all-trans retinal dimer (ATRD), A2-glycerophospho ethanolamine (A2GPE), dihydropyridine phosphatidyl ethanolamine (A2DHPE) and monofuran A2E (MFA2E) in age matched retina. METHODS: The development of microwave assisted synthesis of A2E, its purification and characterization for its utility in quantification in human retina...
November 26, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Gabriel Beikircher, Walter Pulverer, Manuela Hofner, Christa Noehammer, Andreas Weinhaeusel
DNA methylation is a chemically stable key-player in epigenetics. In the vertebrate genome the 5-methyl cytosine (5mC) has been found almost exclusively in the CpG dinucleotide context. CpG dinucleotides are enriched in CpG islands very frequently located within or close to gene promoters. Analyses of DNA methylation changes in human diagnostics have been conducted classically using methylation-sensitive restriction enzymes (MSRE). Since the discovery of bisulfite conversion-based sequencing and PCR assays, MSRE-based PCR assays have been less frequently used, although especially in the field of cancer epigenetics MSRE-based genome-wide discovery and targeted screening applications have been and are still performed successfully...
2018: Methods in Molecular Biology
Wei-Ming Cheng, Yu-Hua Fan, Alex T L Lin
BACKGROUND: The aim of the study was to identify the impact of non-bladder co-morbid conditions on the urodynamic characteristics of patients with bladder pain syndrome/interstitial cystitis. METHODS: Patients with bladder pain syndrome/interstitial cystitis completed the screening questionnaires for chronic fatigue syndrome, irritable bowel syndrome, fibromyalgia, temporo-mandibular disorders, multiple chemical sensitivities, tension/migraine headache, and localized myofascial pain disorder...
December 6, 2017: Journal of the Chinese Medical Association: JCMA
Yunan Luo, Sheng Wang, Jinfeng Xiao, Jian Peng
A variety of large-scale pharmacogenomic data, such as perturbation experiments and sensitivity profiles, enable the systematical identification of drug mechanism of actions (MoAs), which is a crucial task in the era of precision medicine. However, integrating these complementary pharmacogenomic datasets is inherently challenging due to the wild heterogeneity, high-dimensionality and noisy nature of these datasets. In this work, we develop Mania, a novel method for the scalable integration of large-scale pharmacogenomic data...
2018: Pacific Symposium on Biocomputing
Zhi Sun, Lihua Zuo, Tongwen Sun, Jinfa Tang, Daling Ding, Lin Zhou, Jian Kang, Xiaojian Zhang
To clarify and quantify the chemical profiling of XueBiJing injection (XBJ) rapidly, a feasible and accurate strategy was developed by applying ultra high performance liquid chromatography-Q Exactive hybrid quadrupole-orbitrap high resolution accurate mass spectrometry (UHPLC-Q-Orbitrap HRMS). A total of 162 components were characterized, including 19 phenanthrenequinones, 33 lactones, 28 flavonoids and 12 phenolic acids and 51 other compounds. Among them, 38 major compounds were unambiguously quantified by comparing with reference standards...
December 5, 2017: Scientific Reports
Shiho Tokonami, Emi Shimizu, Mamoru Tamura, Takuya Iida
Molecular imprinting technique enables the selective binding of nanoscale target molecules to a polymer film, within which their chemical structure is transcribed. Here, we report the successful production of mixed bacterial imprinted film (BIF) from several food poisoning bacteria by the simultaneous imprinting of their nanoscale surface chemical structures (SCS), and provide highly selective trapping of original micron-scale bacteria used in the production process of mixed BIF even for multiple kinds of bacteria in real samples...
November 30, 2017: Scientific Reports
Anupam Dey, Debashis Barik
Cellular differentiations are often regulated by bistable switches resulting from specific arrangements of multiple positive feedback loops (PFL) fused to one another. Although bistability generates digital responses at the cellular level, stochasticity in chemical reactions causes population heterogeneity in terms of its differentiated states. We hypothesized that the specific arrangements of PFLs may have evolved to minimize the cellular heterogeneity in differentiation. In order to test this we investigated variability in cellular differentiation controlled either by parallel or serial arrangements of multiple PFLs having similar average properties under extrinsic and intrinsic noises...
2017: PloS One
J H Lee, S Basith, M Cui, B Kim, S Choi
The cytochrome P450 (CYP) enzyme superfamily is involved in phase I metabolism which chemically modifies a variety of substrates via oxidative reactions to make them more water-soluble and easier to eliminate. Inhibition of these enzymes leads to undesirable effects, including toxic drug accumulations and adverse drug-drug interactions. Hence, it is necessary to develop in silico models that can predict the inhibition potential of compounds for different CYP isoforms. This study focused on five major CYP isoforms, including CYP1A2, 2C9, 2C19, 2D6 and 3A4, that are responsible for more than 90% of the metabolism of clinical drugs...
October 2017: SAR and QSAR in Environmental Research
Qiang Zhang, Yue Su, Xiaopan Liu, Yinlong Guo
RATIONALE: The direct detection of nonpolar and low-polarity solvent extracts of herbal medicine is difficulty by conventional electrospray ionization mass spectrometry (ESI-MS). This problem can be solved by solvent-assisted electrospray ionization mass spectrometry (SAESI-MS). With the help of assisted solvents (ESI-friendly solvents) at the tip of the spray needle, compounds (especially the low-polarity compounds) in nonpolar and low-polarity solvent extracts can be ionized directly...
November 25, 2017: Rapid Communications in Mass Spectrometry: RCM
Jia Wu, Wenzhi Yang, Huiqin Pan, Shuai Yao, Wanying Wu, Dean Guo
The geographic impact on the quality of Alismatis Rhizoma (derived from the tuber of Alisma orientale), a reputable diuretic traditional Chinese medicine, has seldom been evaluated. Here a metabolomics-driven approach targeting the bioactive protostane triterpenes was developed, by incorporating ultra high performance liquid chromatography with quadrupole time-of-flight mass spectrometry based untargeted metabolite profiling and multiple reaction monitoring quantitative assay, to probe the triterpene differences between Alismatis Rhizoma samples collected from Sichuan, Fujian, and Jiangxi Provinces...
November 27, 2017: Journal of Separation Science
Alexandra S Long, John W Wills, Dorothy Krolak, Matthew Guo, Stephen D Dertinger, Volker M Arlt, Paul A White
Genetic damage is a key event in tumorigenesis, and chemically induced genotoxic effects are a human health concern. Although genetic toxicity data have historically been interpreted using a qualitative screen-and-bin approach, there is increasing interest in quantitative analysis of genetic toxicity dose-response data. We demonstrate an emerging use of the benchmark dose (BMD)-approach for empirically ranking cross-tissue sensitivity. Using a model environmental carcinogen, we quantitatively examined responses for four genetic damage endpoints over an extended dose range, and conducted cross-tissue sensitivity rankings using BMD100 values and their 90% confidence intervals (CIs)...
November 24, 2017: Archives of Toxicology
Miao Pei, Xiangyu Zhu, Xiaojia Huang
Effective extraction is a key step in the determination of sulfonylurea herbicides (SUHs) in complicated samples. According to the chemical properties of SUHs, a new monolithic adsorbent utilizing acrylamidophenylboronic acid and vinylimidazole as mixed functional monomers was synthesized. The new adsorbent was employed as the extraction phase of multiple monolithic fiber solid-phase microextraction (MMF-SPME) of SUHs, and the extracted SUHs were determined by high-performance liquid chromatography with diode array detection (HPLC-DAD)...
November 15, 2017: Journal of Chromatography. A
Mihajlo Novakovic, Ricardo P Martinho, Gregory L Olsen, Michael S Lustig, Lucio Frydman
Chemical exchange saturation transfer (CEST) experiments enhance the NMR signals of labile protons by continuously transferring these protons' saturation to an abundant solvent pool like water. The present study expands these principles by fusing into these experiments homonuclear isotropic mixing sequences, enabling the water-enhanced detection of non-exchangeable species. Further opportunities are opened by the addition of coupling-mediated heteronuclear polarization transfers, which then impose on the water resonance a saturation stemming from non-labile heteronuclear species like (13)C...
November 24, 2017: Physical Chemistry Chemical Physics: PCCP
Shlomit Dachir, Ishai Rabinovitz, Guy Yaacov, Hila Gutman, Liat Cohen, Vered Horwitz, Maayan Cohen, Tamar Kadar
Sulfur mustard (SM) is an incapacitating chemical warfare agent used in numerous conflicts around the world and it is still a major threat for both, army troops and civilians. To evaluate its multiple targets effects in experimental setup, a model of whole body exposure (WBE) to SM vapor was established in rats and its simultaneous effects on lungs and eyes as well as on general wellbeing were examined. Rats were exposed to SM vapor. Evaluation (up to 10 weeks post-exposure) included body weight, general observation, blood counts and histological analysis...
November 24, 2017: Drug and Chemical Toxicology
Chenguang He, Wei Zhao, Kang Zhang, Longfei He, Hualong Wu, Ningyang Liu, Shan Zhang, Xiaoyan Liu, Zhitao Chen
It is widely believed that the lack of high-quality GaN wafers severely hinders the progress in GaN-based devices, especially for defect-sensitive devices. Here, low-cost AlN buffer layers were sputtered on cone-shaped patterned sapphire substrates (PSSs) to obtain high-quality GaN epilayers. Without any mask or regrowth, facet-controlled epitaxial lateral overgrowth (FACELO) was realized by metal-organic chemical vapor deposition (MOCVD). The uniform coating of sputtered AlN buffer layer and the optimized multiple-modulation guaranteed the high growth selectivity and uniformity of GaN epilayer...
November 22, 2017: ACS Applied Materials & Interfaces
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