keyword
https://read.qxmd.com/read/38468162/the-relationship-between-primary-ovarian-insufficiency-and-gene-variations-a-prospective-case-control-study
#21
JOURNAL ARTICLE
Reyhan Gündüz, Selahaddin Tekeş, Lütfiye Ozpak, Elif Ağaçayak, Senem Yaman Tunç, Mehmet Sait İcen, Mehmet Sıddık Evsen
Around 70 percent of cases of Primary Ovarian Insufficiency (POI) etiology remain unexplained. The aim of our study is to contribute to the etiology and genetic background of POI. A total of 37 POI patients and 30 women in the reproductive period were included in this prospective, case-control study between August 2020 and December 2021. The women were examined for 36 genes with next-generation sequencing (NGS) panel. Gene variations were detected in 59.5 percent of the patients in the case group. FSHR p.S680N (rs6166, c...
March 11, 2024: Women & Health
https://read.qxmd.com/read/38468091/genetic-code-expansion-in-pseudomonas-putida-kt2440
#22
JOURNAL ARTICLE
Tianyu Gao, Jiantao Guo, Wei Niu
Emerging microorganism Pseudomonas putida KT2440 is utilized for the synthesis of biobased chemicals from renewable feedstocks and for bioremediation. However, the methods for analyzing, engineering, and regulating the biosynthetic enzymes and protein complexes in this organism remain underdeveloped.Such attempts can be advanced by the genetic code expansion-enabled incorporation of noncanonical amino acids (ncAAs) into proteins, which also enables further controls over the strain's biological processes. Here, we give a step-by-step account of the incorporation of two ncAAs into any protein of interest (POI) in response to a UAG stop codon by two commonly used orthogonal archaeal tRNA synthetase and tRNA pairs...
2024: Methods in Molecular Biology
https://read.qxmd.com/read/38465944/dynamic-light-induced-protein-patterns-at-model-membranes
#23
JOURNAL ARTICLE
Daniele Di Iorio, Seraphine V Wegner
The precise localization and activation of proteins at the cell membrane at a certain time gives rise to many cellular processes, including cell polarization, migration, and division. Thus, methods to recruit proteins to model membranes with subcellular resolution and high temporal control are essential when reproducing and controlling such processes in synthetic cells. Here, a method is described for fabricating light-regulated reversible protein patterns at lipid membranes with high spatiotemporal precision...
February 23, 2024: Journal of Visualized Experiments: JoVE
https://read.qxmd.com/read/38460951/application-and-development-of-a-taqman-based-real-time-pcr-assay-for-rapid-detection-of-snakehead-vesiculovirus
#24
JOURNAL ARTICLE
Cuiping Gong, Panpan Zhu, Jiaxin Ye, Jianfeng Lou, Liwen Zhang, Xiaodan Liu, Weiguang Kong
Snakehead vesiculovirus (SHVV) is one of the primary pathogens responsible for viral diseases in the snakehead fish. A TaqMan-based real-time PCR assay was established for the rapid detection and quantification of SHVV in this study. Specific primers and fluorescent probes were designed for phosphoprotein (P) gene, and after optimizing the reaction conditions, the results indicated that the detection limit of this method could reach 37.1 copies, representing a 100-fold increase in detection sensitivity compared to RT-PCR...
March 9, 2024: FEMS Microbiology Letters
https://read.qxmd.com/read/38445591/tyrosinase-based-proximity-labeling-in-living-cells-and-in-vivo
#25
JOURNAL ARTICLE
Hao Zhu, Jae Hoon Oh, Yuna Matsuda, Takeharu Mino, Mamoru Ishikawa, Hideki Nakamura, Muneo Tsujikawa, Hiroshi Nonaka, Itaru Hamachi
Characterizing the protein constituents of a specific organelle and protein neighbors of a protein of interest (POI) is essential for understanding the function and state of the organelle and protein networks associated with the POI. Proximity labeling (PL) has emerged as a promising technology for specific and efficient spatial proteomics. Nevertheless, most enzymes adopted for PL still have limitations: APEX requires cytotoxic H2 O2 for activation and thus is poor in biocompatibility for in vivo application, BioID shows insufficient labeling kinetics, and TurboID suffers from high background biotinylation...
March 6, 2024: Journal of the American Chemical Society
https://read.qxmd.com/read/38440129/hypergonadotropic-hypogonadism-due-to-transaldolase-deficiency-two-cases-and-literature-review
#26
Akram Takaleh, Nasser Abunamous, Aisha AlShamsi, Noura Alhassani, Raya Almazrouei
Transaldolase deficiency is a rare autosomal recessive inborn error of carbohydrate metabolism caused by pathogenic/likely pathogenic biallelic mutations in the TALDO1 gene. This disorder is characterized by multisystem involvement with variable phenotypes, including intrauterine growth restriction; dysmorphic features; abnormal skin; hepatosplenomegaly; cytopenia; and cardiac, renal, and endocrine abnormalities. Herein, we present two Emirati patients with hypergonadotropic hypogonadism due to transaldolase deficiency and variable phenotypes of systemic involvement...
March 2024: JCEM Case Rep
https://read.qxmd.com/read/38439857/artificial-intelligence-algorithms-for-predicting-post-operative-ileus-after-laparoscopic-surgery
#27
JOURNAL ARTICLE
Cheng-Mao Zhou, HuiJuan Li, Qiong Xue, Jian-Jun Yang, Yu Zhu
OBJECTIVE: By constructing a predictive model using machine learning and deep learning technologies, we aim to understand the risk factors for postoperative intestinal obstruction in laparoscopic colorectal cancer patients, and establish an effective artificial intelligence-based predictive model to guide individualized prevention and treatment, thus improving patient outcomes. METHODS: We constructed a model of the artificial intelligence algorithm in Python. Subjects were randomly assigned to either a training set for variable identification and model construction, or a test set for testing model performance, at a ratio of 7:3...
March 15, 2024: Heliyon
https://read.qxmd.com/read/38431928/securing-lytac-with-logic-identification-system-for-cancer-cell-selective-membrane-protein-degradation
#28
JOURNAL ARTICLE
Yanyun Fang, Yue Zhang, Shiyi Bi, Bo Peng, Caixia Wang, Huangxian Ju, Ying Liu
Lysosome-targeting chimera (LYTAC) links proteins of interest (POIs) with lysosome-targeting receptors (LTRs) to achieve membrane protein degradation, which is becoming a promising therapeutic modality. However, cancer cell-selective membrane protein degradation remains a big challenge considering expressions of POIs in both cancer cells and normal cells, as well as broad tissue distribution of LTRs. Here a logic-identification system is designed, termed Logic-TAC, based on cell membrane-guided DNA calculations to secure LYTAC selectively for cancer cells...
March 3, 2024: Small
https://read.qxmd.com/read/38428403/ningxin-tongyu-zishen-formula-alleviates-the-senescence-of-granulosa-cells-on-d-galactose-induced-premature-ovarian-insufficiency-mice
#29
JOURNAL ARTICLE
Jia-Wen Ma, Zeng-Yan Xiong, Xing-Chu Cai, Xiang Li, Shi-Yan Ren, Shuai-Qi An, Zai-Yang Zhang, Yi-Zhou Zhang
Ningxin-Tongyu-Zishen formula (NTZF) is a clinical experience formula for the treatment of premature ovarian insufficiency (POI) in traditional Chinese medicine (TCM), and the potential mechanism is unknown. For in vivo experiments, POI mouse models (C57BL/6 mice), were constructed by subcutaneous injection of D-galactose (D-gal, 200 mg/kg). After treatment of NTZF (10.14, 20.27, 40.54 g/kg;) or estradiol valerate (0.15 mg/kg), ovarian function, oxidative stress (OS) and protein expression of Sirt1/p53 were evaluated...
February 28, 2024: Aging
https://read.qxmd.com/read/38428262/pathophysiology-and-management-of-postoperative-ileus-in-adults-and-neonates-a-review
#30
REVIEW
Sindhu Mannava, Attie Vogler, Troy Markel
Postoperative ileus (POI) is caused by enteric neural dysfunction and inflammatory response to the stress of surgery as well as the effect of anesthetics and opioid pain medications. POI results in prolonged hospital stays, increased medical costs, and diminished enteral nutrition, rendering it a problem worth tackling. Many cellular pathways are implicated in this disease process, creating numerous opportunities for targeted management strategies. There is a gap in the literature in studies exploring neonatal POI pathophysiology and treatment options...
February 29, 2024: Journal of Surgical Research
https://read.qxmd.com/read/38423674/brd4-targeting-protacs-synergize-with-chemotherapeutics-against-osteosarcoma-cell-lines
#31
JOURNAL ARTICLE
Clemens Lang, Sandra Stickler, Barbara Rath, Maryana Teufelsbauer, Lukas Weigl, Martin Hohenegger, Gerhard Hamilton
BACKGROUND/AIM: Osteosarcoma at an advanced stage has a poor outcome, and novel targeted therapies are needed, especially for metastatic disease. Bromodomain inhibitors (BETi) are epigenetic modulators that broadly impair the expression of oncogenic proteins and exert antitumor effects. BETi can be combined with chemotherapeutics to increase therapeutic responses with superior effects in the form of proteolysis targeting chimeras (PROTACs) that degrade proteins of interest (POI) in multiple cycles...
March 2024: Anticancer Research
https://read.qxmd.com/read/38420025/optimization-strategy-of-community-planning-for-environmental-health-and-public-health-in-smart-city-under-multi-objectives
#32
JOURNAL ARTICLE
Fujiang Chen, Wuyue She, Feng Zeng, Meiben Gao, Chao Wen, Guoxin Liu, Lixun Wu, Yang Wu, Chi Zhang
As population density increases, environmental hygiene and public health become increasingly severe. As the space where residents stay for the longest time and have the most profound impact on their physical and mental health, the quality of the environment in urban communities largely determines the degree to which residents engage in physical activity, bear the risk of pollution exposure, and obtain healthy food. Therefore, in order to ensure the physical and mental health of residents, this study proposes community planning guided by environmental hygiene and public health, and establishes an environmental health assessment system for this purpose...
2024: Frontiers in Public Health
https://read.qxmd.com/read/38419772/bluetooth-dataset-for-proximity-detection-in-indoor-environments-collected-with-smartphones
#33
JOURNAL ARTICLE
Michele Girolami, Davide La Rosa, Paolo Barsocchi
This paper describes a data collection experiment and the resulting dataset based on Bluetooth beacon messages collected in an indoor museum. The goal of this dataset is to study algorithms and techniques for proximity detection between people and points of interest (POI). To this purpose, we release the data we collected during 32 museum's visits, in which we vary the adopted smartphones and the visiting paths. The smartphone is used to collect Bluetooth beacons emitted by Bluetooth tags positioned nearby each POI...
April 2024: Data in Brief
https://read.qxmd.com/read/38418866/the-impact-of-premature-ovarian-insufficiency-on-sexual-function-which-domain-is-mostly-disrupted
#34
JOURNAL ARTICLE
Maryam Farahmand, Fahimeh Ramezani Tehrani
This study aimed to compare the sexual function (SF) and its domains between women with idiopathic premature ovarian insufficiency (POI) and healthy control. A cross-sectional study was conducted on 63 married women with idiopathic POI, referred to the reproductive endocrinology research center (Tehran, Iran), and 784 married women with regular menstrual cycles who visited for routine annual gynecological exams in 2021-2022. The Female Sexual Function Index (FSFI) questionnaire was used to assess the SF of participants...
February 28, 2024: International Journal of Impotence Research
https://read.qxmd.com/read/38416855/an-ingestible-self-propelling-device-for-intestinal-reanimation
#35
JOURNAL ARTICLE
Shriya S Srinivasan, Julien Dosso, Hen-Wei Huang, George Selsing, Amro Alshareef, Johannes Kuosmanen, Keiko Ishida, Joshua Jenkins, Wiam Abdalla Mohammed Madani, Alison Hayward, Giovanni Traverso
Postoperative ileus (POI) is the leading cause of prolonged hospital stay after abdominal surgery and is characterized by a functional paralysis of the digestive tract, leading to symptoms such as constipation, vomiting, and functional obstruction. Current treatments are mainly supportive and inefficacious and yield acute side effects. Although electrical stimulation studies have demonstrated encouraging pacing and entraining of the intestinal slow waves, no devices exist today to enable targeted intestinal reanimation...
February 28, 2024: Science Robotics
https://read.qxmd.com/read/38404010/plasma-derived-from-human-umbilical-cord-blood-restores-ovarian-function-and-improves-serum-reproductive-hormones-levels-in-mice-with-premature-ovarian-insufficiency-poi-through-cytokines-and-growth-factors
#36
JOURNAL ARTICLE
Tie Cheng Sun, Yi Ming Guo, Dong Mei Li, Yu Jie Wen, Zhu Xue Jiao, Ju Li, Hui Ping Wang, Yan Jun Jia, Shan Jie Zhou
Premature ovarian insufficiency (POI) patients experience a decline in ovarian function and a reduction in serum reproductive hormones, leading to a significant impact on the outcomes of assisted reproductive technology. Despite the absence of an effective clinical treatment to restore fertility in POI patients, recent research has indicated that cord blood plasma (CBP) derived from human umbilical cord blood (hUCB) may offer therapeutic benefits for various degenerative diseases. The primary aim of this study is to explore approaches for enhancing ovarian function and serum reproductive hormones through the administration of CBP in a murine model...
February 2024: Molecular Reproduction and Development
https://read.qxmd.com/read/38400382/persistent-monitoring-for-points-of-interest-with-different-data-update-deadlines
#37
JOURNAL ARTICLE
Qing Guo, Jian Peng
In this paper, we study the regular sensory data collection of Points of Interest (PoIs) with multiple Unmanned Aerial Vehicles (UAVs) during an extended monitoring period, where each PoI is visited multiple times before its data update deadline to keep the data fresh. We observe that most existing studies ignored the important differences in the data stored in the PoIs, scheduled a plan that dispatched UAVs to visit all PoIs before the same deadline, and simply repeated the plan during the monitoring period, which undoubtedly increased the service cost of the UAVs...
February 14, 2024: Sensors
https://read.qxmd.com/read/38400319/an-enhancement-of-outdoor-location-based-augmented-reality-anchor-precision-through-vslam-and-google-street-view
#38
JOURNAL ARTICLE
Komang Candra Brata, Nobuo Funabiki, Yohanes Yohanie Fridelin Panduman, Evianita Dewi Fajrianti
Outdoor Location-Based Augmented Reality (LAR) applications require precise positioning for seamless integrations of virtual content into immersive experiences. However, common solutions in outdoor LAR applications rely on traditional smartphone sensor fusion methods, such as the Global Positioning System (GPS) and compasses, which often lack the accuracy needed for precise AR content alignments. In this paper, we introduce an innovative approach to enhance LAR anchor precision in outdoor environments. We leveraged Visual Simultaneous Localization and Mapping (VSLAM) technology, in combination with innovative cloud-based methodologies, and harnessed the extensive visual reference database of Google Street View (GSV) , to address the accuracy limitation problems...
February 9, 2024: Sensors
https://read.qxmd.com/read/38397479/current-status-and-future-prospects-of-stem-cell-therapy-for-infertile-patients-with-premature-ovarian-insufficiency
#39
REVIEW
Hye Kyeong Kim, Tae Jin Kim
Premature ovarian insufficiency (POI), also known as premature menopause or premature ovarian failure, signifies the partial or complete loss of ovarian endocrine function and fertility before 40 years of age. This condition affects approximately 1% of women of childbearing age. Although 5-10% of patients may conceive naturally, conventional infertility treatments, including assisted reproductive technology, often prove ineffective for the majority. For infertile patients with POI, oocyte donation or adoption exist, although a prevalent desire persists among them to have biological children...
February 19, 2024: Biomolecules
https://read.qxmd.com/read/38396883/trace-dna-transfer-in-co-working-spaces-the-importance-of-background-dna-analysis
#40
JOURNAL ARTICLE
Martina Onofri, Federica Tommolini, Simona Severini, Cristiana Gambelunghe, Massimo Lancia, Luigi Carlini, Eugenia Carnevali
The presence of background DNA (bgDNA) can hinder the evaluation of DNA evidence at the activity level, especially when the suspect is expected to be retrieved due to their habitual occupation of the investigated environment. Based on real-life casework circumstances, this study investigates the prevalence, composition, origin, and probable transfer routes of bgDNA found on personal items in situations where their owner and person of interest (POI) share the same workspace. Baseline values of bgDNA were evaluated on the participants' personal items...
February 12, 2024: International Journal of Molecular Sciences
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