Read by QxMD icon Read

Intervertebral Disc Mechanics

Carla Cunha, Catarina R Almeida, Maria Inês Almeida, Andreia M Silva, Maria Molinos, Sofia Lamas, Catarina L Pereira, Graciosa Q Teixeira, António T Monteiro, Susana G Santos, Raquel M Gonçalves, Mário A Barbosa
: : Cell therapies for intervertebral disc (IVD) regeneration presently rely on transplantation of IVD cells or stem cells directly to the lesion site. Still, the harsh IVD environment, with low irrigation and high mechanical stress, challenges cell administration and survival. In this study, we addressed systemic transplantation of allogeneic bone marrow mesenchymal stem cells (MSCs) intravenously into a rat IVD lesion model, exploring tissue regeneration via cell signaling to the lesion site...
October 11, 2016: Stem Cells Translational Medicine
Gauri Tendulkar, Phillip Grau, Patrick Ziegler, Alfred Buck, Alfred Buck, Andreas Badke, Hans-Peter Kaps, Sabrina Ehnert, Andreas K Nussler
Intervertebral disc degeneration and disc herniation is one of the major causes of lower back pain. Depletion of extracellular matrix, culminating in nucleus pulposus (NP) extrusion leads to intervertebral disc destruction. Currently available surgical treatments reduce the pain but do not restore the mechanical functionality of the spine. In order to preserve mechanical features of the spine, total disc or nucleus replacement thus became a wide interest. However, this arthroplasty era is still in an immature state, since none of the existing products have been clinically evaluated...
2016: Journal of Visualized Experiments: JoVE
Rob C Brink, Tom P C Schlösser, Dino Colo, Ludvig Vavruch, Marijn van Stralen, Koen L Vincken, Marcus Malmqvist, Moyo C Kruyt, Hans Tropp, René M Castelein
STUDY DESIGN: Cross-sectional. OBJECTIVE: To investigate the presence and magnitude of anterior spinal overgrowth in neuromuscular scoliosis and compare this to the same measurements in idiopathic scoliosis and healthy spines. SUMMARY OF BACKGROUND DATA: Anterior spinal overgrowth has been described as a potential driver for the onset and progression of adolescent idiopathic scoliosis (AIS). Whether this anterior overgrowth is specific for AIS or also present in non-idiopathic scoliosis has not been reported...
September 28, 2016: Spine
Simon Wan, Samantha Borland, Stephen M Richardson, Catherine L R Merry, Alberto Saiani, Julie E Gough
: Cell-based therapies for regeneration of intervertebral discs are regarded to hold promise for degenerative disc disease treatment, a condition that is strongly linked to lower back pain. A de novo self-assembling peptide hydrogel (SAPH), chosen for its biocompatibility, tailorable properties and nanofibrous architecture, was investigated as a cell carrier and scaffold for nucleus pulposus (NP) tissue engineering. Oscillatory rheology determined that the system would likely be deliverable via minimally invasive procedure and mechanical properties could be optimised to match the stiffness of the native human NP...
September 24, 2016: Acta Biomaterialia
Jérôme Molimard, Rebecca Bonnaire, Reynald Convert, Woo Suck Han, Paul Calmels
OBJECTIVE: Although it is only a part of their therapeutic effect, the mechanical effect of compression or contention medical devices (CCMD) is always claimed by the manufacturers. However, the mechanism between the pressure application zone and the targeted organ is complex. It involves a purely passive mechanical effect and mechanisms related to the tonic postural system. Various strategies can be implemented to show the effectiveness of a mechanical action; among them, biomechanical modeling is a able to consider complex mechanical effects before any clinical trial...
September 2016: Annals of Physical and Rehabilitation Medicine
M M van Rijsbergen, V M P Barthelemy, A C T Vrancken, S P M Crijns, H-J Wilke, W Wilson, B van Rietbergen, K Ito
The two main load bearing tissues of the intervertebral disc are the nucleus pulposus and the annulus fibrosus. Both tissues are composed of the same basic components, but differ in their organization and relative amounts. With degeneration, the clear distinction between the two tissues disappears. The changes in biochemical content lead to changes in mechanical behaviour of the intervertebral disc. The aim of the current study was to investigate if well-documented moderate degeneration at the biochemical and fibre structure level leads to instability of the lumbar spine...
September 23, 2016: Biomechanics and Modeling in Mechanobiology
D B Amin, I M Lawless, D Sommerfeld, R M Stanley, B Ding, J J Costi
The complex, direction-dependent, poro-viscoelastic properties of the intervertebral disc (disc) suggest that investigations of the six degree of freedom (6DOF) behaviour may be susceptible to inter-test variation in mechanical response if the disc does not return to initial conditions between loading directions. No studies have quantified the effects of sequential multi-directional loading on the consistency of the compressive response of the disc throughout a 6DOF testing protocol. Therefore, the objective of this study was to determine the effect of 6DOF loading on the compressive properties (stiffness and phase angle) of human discs, as evaluated by a reference compression test performed after each single DOF test...
October 3, 2016: Journal of Biomechanics
Frederic Shapiro
Each of the radiographic, histologic, whole mount, and three-dimensional computerized reconstruction techniques throws specific light on the vertebral, intervertebral disc, and rib abnormalities in the development of the pudgy mouse. The end result demonstrates a failure of both normal formation and normal segmentation in the pudgy mouse recognized now as a recessive genetic disorder in which mutations in the Delta-like 3 gene (Dll3) have been defined [2]. The variable appearance of the vertebral and rib abnormalities from mouse to mouse, including in particular the variable appearance in affected littermates, indicates that the gene abnormality alone does not account solely for the deformities seen...
2016: Advances in Anatomy, Embryology, and Cell Biology
Hendrik Schmidt, Sandra Reitmaier, Friedmar Graichen, Aboulfazl Shirazi-Adl
By maintaining a balance between external mechanical loads and internal osmotic pressure, fluid content of intervertebral discs constantly alters causing fluctuations in disc hydration, height, diameter and pressure that govern disc temporal response. This paper reviews and discusses the relevant findings of earlier studies on the disc fluid flow with the aim to understand and remedy discrepancies between in vivo and in vitro observations. New results of finite element model studies are also exploited in order to help identify the likely causes for such differences and underlying mechanisms observed in vitro...
October 3, 2016: Journal of Biomechanics
Li-Guo Zhu, Min-Shan Feng, Jia-Wen Zhan, Ping Zhang, Jie Yu
BACKGROUND: The development of mechanically active culture systems helps increase the understanding of the role of mechanical stress in intervertebral disc (IVD) degeneration. Motion segment cultures allow for preservation of the native IVD structure, and adjacent vertebral bodies facilitate the application and control of mechanical loads. The purpose of this study was to establish loading and organ culture methods for rabbit IVD motion segments to study the effect of static load on the whole disc organ...
2016: Chinese Medical Journal
Yi Feng, Brian Egan, Jinxi Wang
Low back pain (LBP) is a major cause of disability and imposes huge economic burdens on human society worldwide. Among many factors responsible for LBP, intervertebral disc degeneration (IDD) is the most common disorder and is a target for intervention. The etiology of IDD is complex and its mechanism is still not completely understood. Many factors such as aging, spine deformities and diseases, spine injuries, and genetic factors are involved in the pathogenesis of IDD. In this review, we will focus on the recent advances in studies on the most promising and extensively examined genetic factors associated with IDD in humans...
September 2016: Genes & Diseases
Wai Hon Chooi, Samantha Chun Wai Chan, Benjamin Gantenbein, Barbara Pui Chan
Mechanical loading has been shown to affect cell viability and matrix maintenance in the intervertebral disc (IVD) but there is no investigation on how cells survive mechanical stress and whether the IVD cells perceive mechanical loading as stress and respond by expression of heat shock proteins. This study investigates the stress response in the IVD in response to compressive loading. Bovine caudal disc organ culture was used to study the effect of physiological range static loading and dynamic loading. Cell activity, gene expression and immunofluorescence staining were used to analyze the cell response...
2016: PloS One
ZhanJun Yan, YouDong Pan, ShiHui Wang, MaoHua Cheng, HongMei Kong, ChunGuang Sun, Kai Hu, TongLei Chen, QiRong Dong, JianFeng Chen
STUDY DESIGN: A 3-level rat tail caudal intervertebral disc (IVD) degeneration (IVDD) model was established to study effects of static compression on extracellular matrix (ECM) remodeling and integrin signaling in IVDs during IVDD. OBJECTIVE: To investigate the effect of compression force on ECM remodeling and integrin signaling in IVDs during IVDD SUMMARY OF BACKGROUND DATA.: Integrins sense mechanical environment alteration via binding to ECM ligands and trigger intracellular signaling for pathological ECM remodeling during IVDD...
August 19, 2016: Spine
F Javier Fonseca Del Pozo, Joaquín Valle Alonso, Miguel Ángel Caracuel Ruiz, Siyamini Vythilingam, Daniel Lopez Ruiz
Vertebral osteomyelitis, or spondylodiscitis, is a rare disease with increasing prevalence in recent years due to a greater number of spinal surgical procedures, nosocomial bacteraemia, an aging population and intravenous drug addiction. Haematogenous infection is the most common cause of spondylodiscitis. We report a 47-year-old man diagnosed with Escherichia coli spondylodiscitis. The patient initially presented with a 4-day history of inflammatory, mechanical pain in the lower back suggesting sciatica. Treatment included NSAIDs and opioids...
July 2016: Bulletin of Emergency and Trauma
Ryo Nakamichi, Yoshiaki Ito, Masafumi Inui, Naoko Onizuka, Tomohiro Kayama, Kensuke Kataoka, Hidetsugu Suzuki, Masaki Mori, Masayo Inagawa, Shizuko Ichinose, Martin K Lotz, Daisuke Sakai, Koichi Masuda, Toshifumi Ozaki, Hiroshi Asahara
The main pathogenesis of intervertebral disc (IVD) herniation involves disruption of the annulus fibrosus (AF) caused by ageing or excessive mechanical stress and the resulting prolapse of the nucleus pulposus. Owing to the avascular nature of the IVD and lack of understanding the mechanisms that maintain the IVD, current therapies do not lead to tissue regeneration. Here we show that homeobox protein Mohawk (Mkx) is a key transcription factor that regulates AF development, maintenance and regeneration. Mkx is mainly expressed in the outer AF (OAF) of humans and mice...
2016: Nature Communications
Rogerio Carlos Sanfelice Nunes, Elenir Rose Jardim Cury Pontes, Izaias Pereira da Costa
OBJECTIVE: Sciatic pain secondary to lumbar disc herniation is a complex condition that is often highly limiting. The causes of pain in disc herniation are multifactorial. Two physiopathological mechanisms are involved in discogenic pain: mechanical deformation of nerve roots and a biochemical inflammatory component resulting from contact between the intervertebral disc and neural tissue, by way of the nucleus pulposus. The aim of this study was to evaluate the efficacy and safety of epidural blockade as therapy for bulging lumbar disc herniation...
July 2016: Revista Brasileira de Ortopedia
Fang Fang, Dianming Jiang
Inflammation and cytokines have been recognized to correlate with intervertebral disc (IVD) degeneration (IDD), via mediating the development of clinical signs and symptoms. However, the regulation mechanism remains unclear. We aimed at investigating the regulatory role of interleukin (IL)β and high mobility group box 1 (HMGB1) in the inflammatory response in human IVD cells, and then explored the signalling pathways mediating such regulatory effect. Firstly, the promotion to inflammatory cytokines in IVD cells was examined with ELISA method...
October 2016: Bioscience Reports
Christopher Fernandez, Alan Marionneaux, Sanjitpal Gill, Jeremy Mercuri
Intervertebral disc (IVD) degeneration (IDD) and herniation (IDH) can result in low back pain and impart significant socioeconomic burden. These pathologies involve detrimental alteration to the nucleus pulposus (NP) either via biochemical degradation or extrusion from the IVD, respectively. Thus, engineering living NP tissue utilizing biomaterial scaffolds that recapitulate native NP microarchitecture, biochemistry, mechanical properties and which support cell viability represents an approach to aiding patients with IDD and IDH...
August 10, 2016: Journal of Biomedical Materials Research. Part A
Michal Dudek, Nan Yang, Jayalath Pd Ruckshanthi, Jack Williams, Elzbieta Borysiewicz, Ping Wang, Antony Adamson, Jian Li, John F Bateman, Michael R White, Raymond P Boot-Handford, Judith A Hoyland, Qing-Jun Meng
OBJECTIVES: The circadian clocks are internal timing mechanisms that drive ∼24-hour rhythms in a tissue-specific manner. Many aspects of the physiology of the intervertebral disc (IVD) show clear diurnal rhythms. However, it is unknown whether IVD tissue contains functional circadian clocks and if so, how their dysregulation is implicated in IVD degeneration. METHODS: Clock gene dynamics in ex vivo IVD explants (from PER2:: luciferase (LUC) reporter mice) and human disc cells (transduced with lentivirus containing Per2::luc reporters) were monitored in real time by bioluminescence photon counting and imaging...
August 3, 2016: Annals of the Rheumatic Diseases
Masoud Abdollahi, Mohammad Nikkhoo, Sajad Ashouri, Mohsen Asghari, Mohamad Parnianpour, Kinda Khalaf
This study developed and validated a lumped parameter model for the FLEXI-BAR, a popular training instrument that provides vibration stimulation. The model which can be used in conjunction with musculoskeletal-modeling software for quantitative biomechanical analyses, consists of 3 rigid segments, 2 torsional springs, and 2 torsional dashpots. Two different sets of experiments were conducted to determine the model's key parameters including the stiffness of the springs and the damping ratio of the dashpots...
October 2016: Medical Engineering & Physics
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"