keyword
https://read.qxmd.com/read/38492411/surfactant-based-supramolecular-dye-assembly-a-highly-selective-and-economically-viable-platform-for-quantification-of-heparin-antidote
#1
JOURNAL ARTICLE
Soumyadeep Sarkar, Goutam Chakraborty, Haridas Pal
Herein, we have employed a supramolecular assembly of a cationic dye, LDS-698 and a common surfactant sodium dodecyl sulfate (SDS) as a turn-on fluorescent sensor for protamine (Pr) detection. Addition of cationic Pr to the solution of dye-surfactant complex brings negatively charged SDS molecules together through strong electrostatic interaction, assisting aggregation of SDS way before its critical micellar concentration (CMC). These aggregates encapsulate the dye molecules within their hydrophobic region, arresting non-radiative decay channels of the excited dye...
March 12, 2024: Colloids and Surfaces. B, Biointerfaces
https://read.qxmd.com/read/38481696/enhancing-rna-payload-and-temperature-stability-and-activity-with-cationic-peptide-coated-zinc-oxide-nanoparticles
#2
JOURNAL ARTICLE
Robert K DeLong, Juliet Nava-Chavez, Rakshith Kumar, Elza Neelima Mathew, Waithaka Mwangi, Sunyoung Yoon
The lipid nanoparticle (LNP) mRNA vaccine was first tested through clinic but suffered from relatively low RNA payloads and poor temperature stability. Our lab patented a protamine-coated particle approach for temperature-stabilizing DNA vaccines, translating this successfully to the clinic. In subsequent work, we have characterized RNA interaction and delivery by zinc oxide nanoparticles, filing a patent most recently entitled RNA-stabilizing nanoparticles, similarly utilizing protamine-coated zinc oxide nanoparticles for RNA...
March 8, 2024: ACS Pharmacology & Translational Science
https://read.qxmd.com/read/38480649/effect-of-advanced-glycation-end-products-ages-on-sperm-parameters-and-function-in-c57bl-6-mice
#3
JOURNAL ARTICLE
Zahra Darmishonnejad, Vahideh Hassan Zadeh, Marziyeh Tavalaee, Farzad Kobarfard, Mahsa Hassani, Parviz Gharagozloo, Joël R Drevet, Mohammad Hossein Nasr-Esfahani
This study investigated the deleterious impact of advanced glycation end products (AGEs), commonly present in metabolic disorders like diabetes, obesity, and infertility-related conditions, on sperm structure and function using a mouse model where AGE generation was heightened through dietary intervention. Five-week-old C57BL/6 mice were divided into two groups, one on a regular diet (control) and the other on an AGE-rich diet. After 13 weeks, various parameters were examined, including fasting blood glucose, body weight, food consumption, sperm parameters and function, testicular superoxide dismutase levels, malondialdehyde content, total antioxidant capacity, Johnson score, AGE receptor (RAGE) content, and carboxymethyl lysine (CML) content...
March 13, 2024: Reproductive Sciences
https://read.qxmd.com/read/38478501/melatonin-serves-as-a-novel-treatment-in-bladder-fibrosis-through-tgf-%C3%AE-1-smad-and-emt
#4
JOURNAL ARTICLE
Yang Zhang, Sun Gong, Weixin He, Jie Yuan, Di Dong, Jialong Zhang, Haomin Wang, Binghai Chen
BACKGROUND: Melatonin (MEL) is an indole amine molecule primarily produced in the pineal gland. Melatonin has been shown in numerous studies to have antifibrotic effects on the kidney, liver, and other organs. However, it is still unclear how melatonin works in bladder fibrosis. We explored how melatonin affects animals with bladder fibrosis and the underlying mechanisms. MATERIALS AND METHODS: MEL was used to treat human bladder smooth muscle cells (HBdSMCs) after they were stimulated with transforming growth factor-β1 (TGF-β1) in vitro...
2024: PloS One
https://read.qxmd.com/read/38467105/congenital-zika-virus-infection-impacts-on-male-mice-offspring-s-reproductive-biology
#5
JOURNAL ARTICLE
Natália Teixeira Wnuk, André Felipe Almeida Figueiredo, Talita De Oliveira Farias, Marcos Rocha Gouvêa Brener, Samyra Maria Dos Santos Nassif Lacerda, Vidyleison Neves Camargos, Paulo Henrique Amaral, Lidia Maria Andrade, Maria Ivonete Nogueira Silva, Roberta Araujo Lopes, Raphael Escorsim Szawka, Juan Carlos González, Mauro Martins Teixeira, Danielle da Glória Souza, Vivian Vasconcelos Costa, Guilherme Mattos Jardim Costa
Infection with ZIKV during pregnancy is associated with fetal developmental problems. Although neurological issues are being explored more in experimental studies, limited research has focused on the reproductive health consequences for offspring born to infected mothers. In this context, this study aimed to assess the impact of ZIKV infection during pregnancy on the testes and sperm of adult male offspring. Female mice were intraperitoneally inoculated with a Brazil strain of ZIKV during the 5.5th day of embryonic gestation...
March 1, 2024: Reproduction
https://read.qxmd.com/read/38452081/prophage-proteins-alter-long-noncoding-rna-and-dna-of-developing-sperm-to-induce-a-paternal-effect-lethality
#6
JOURNAL ARTICLE
Rupinder Kaur, Angelina McGarry, J Dylan Shropshire, Brittany A Leigh, Seth R Bordenstein
The extent to which prophage proteins interact with eukaryotic macromolecules is largely unknown. In this work, we show that cytoplasmic incompatibility factor A (CifA) and B (CifB) proteins, encoded by prophage WO of the endosymbiont Wolbachia, alter long noncoding RNA (lncRNA) and DNA during Drosophila sperm development to establish a paternal-effect embryonic lethality known as cytoplasmic incompatibility (CI). CifA is a ribonuclease (RNase) that depletes a spermatocyte lncRNA important for the histone-to-protamine transition of spermiogenesis...
March 8, 2024: Science
https://read.qxmd.com/read/38438264/prolonged-coagulation-times-in-severe-fever-with-thrombocytopenia-syndrome-virus-infection-the-indicators-of-heparin-like-effect-and-increased-haemorrhagic-risk
#7
JOURNAL ARTICLE
Ning Tang, Peihong Yuan, Ming Luo, Dengju Li
Prolonged coagulation times, such as activated partial thromboplastin time (APTT) and thrombin time (TT), are common in patients infected with severe fever with thrombocytopenia syndrome virus (SFTSV) and have been confirmed to be related to patient's poor outcome by previous studies. To find out the reason for prolonged coagulation time in patients with SFTSV infection, and whether it predicts haemorrhagic risk or not. Seventy-eight consecutive patients with confirmed SFTSV infection were enrolled in this prospective, single-centre, observational study...
March 4, 2024: British Journal of Haematology
https://read.qxmd.com/read/38423473/systemic-delivery-of-proteins-using-novel-peptides-via-the-sublingual-route
#8
JOURNAL ARTICLE
Jiamin Wu, Natalie Jones, Lukas Hohenwarter, Feng Zhao, Vanessa Chan, Zheng Tan, Tiffany Carlaw, Tessa Morin, Jing Li, Tejinder Kaur, Lucas J Andrew, Colin J D Ross, Sarah Hedtrich, Shyh-Dar Li
Therapeutic proteins often require needle-based injections, which compromise medication adherence especially for those with chronic diseases. Sublingual administration provides a simple and non-invasive alternative. Herein, two novel peptides (lipid-conjugated protamine and a protamine dimer) were synthesized to enable sublingual delivery of proteins through simple physical mixing with the payloads. It was found that the novel peptides promoted intracellular delivery of proteins via increased pore formation on the cell surface...
March 4, 2024: Journal of Controlled Release
https://read.qxmd.com/read/38420528/advancing-cardiotomy-suction-practices-for-coronary-surgery-via-multidisciplinary-collaborative-learning
#9
JOURNAL ARTICLE
James W Stewart, Donald Nieter, Xiaoting Wu, Alphonse DeLucia, Brittney N Graebner, Gaetano Paone, David C Fitzgerald, Timothy A Dickinson, Min Zhang, Francis D Pagani, Donald S Likosky
OBJECTIVE: Professional standards recommend stopping cardiotomy suction at the termination of cardiopulmonary bypass before protamine administration based on perceived safety concerns. This study evaluated a multidisciplinary collaborative quality-improvement intervention promoting this agreed-upon cardiotomy suction practice during coronary artery bypass grafting (CABG). METHODS: A statewide intervention (eg, unblinded surgeon and perfusionist feedback, evidence-based lectures, evaluating barriers to change) involved 32 centers participating in the PERForm (ie, Perfusion Measures and Outcomes) Registry to standardize cardiotomy suction practices at cardiopulmonary bypass termination during CABG...
February 2024: JTCVS open
https://read.qxmd.com/read/38408323/protamines-and-the-sperm-nuclear-basic-proteins-pandora-s-box-of-insects
#10
JOURNAL ARTICLE
Melissa R Leyden, Brent Gowen, Rodrigo Gonzalez-Romero, Jose Maria Eirin-Lopez, Bo-Hyun Kim, Fumio Hayashi, Jay McCartney, Patrick C Zhang, Miyoko Kubo-Irie, Jeffrey Shabanowitz, Donald F Hunt, Patrick Michael Ferree, Harold Kasinsky, Juan Ausio
Insects are the largest group of animals when it comes to the number and diversity of species. Yet, with the exception of Drosophila, no information is currently available on the primary structure of their sperm nuclear basic proteins (SNBPs). This paper represents the first attempt in this regard and provides information about six species of Mecoptera: Poecillimon thessalicus, Graptosaltria nigrofuscata, Apis mellifera, Nasonia vitripennis, Parachauliodes continentalis, and Tribolium castaneum. The SNBPs of these species were characterized by acetic acid urea gel electrophoresis (AU-PAGE) and HPLC fractionated...
February 26, 2024: Biochemistry and Cell Biology
https://read.qxmd.com/read/38407934/caltrop-like-small-molecule-antidotes-that-neutralize-unfractionated-heparin-and-low-molecular-weight-heparin-in-vivo
#11
JOURNAL ARTICLE
Yang Zong, Zhuo Lei, Shang-Bo Yu, Ling-Yu Zhang, Yan Wu, Ke Feng, Qiao-Yan Qi, Yamin Liu, Yajie Zhu, Peng Guo, Wei Zhou, Dan-Wei Zhang, Zhan-Ting Li
Unfractionated heparin (UFH) and low-molecular-weight heparins (LMWHs) are widely applied for surgical procedures and extracorporeal therapies, which, however, suffer bleeding risk. Protamine, the only clinically approved antidote, can completely neutralize UFH, but only partially neutralizes LMWHs, and also has a number of safety drawbacks. Here, we show that caltrop-like multicationic small molecules can completely neutralize both UFH and LMWHs. In vitro and ex vivo assays with plasma and whole blood and in vivo assays with mice and rats support that the lead compound is not only superior to protamine by displaying higher neutralization activity and broader therapeutic windows but also biocompatible...
February 26, 2024: Journal of Medicinal Chemistry
https://read.qxmd.com/read/38396632/application-of-flow-cytometry-using-advanced-chromatin-analyses-for-assessing-changes-in-sperm-structure-and-dna-integrity-in-a-porcine-model
#12
JOURNAL ARTICLE
Estíbaliz Lacalle, Estela Fernández-Alegre, Belén Gómez-Giménez, Manuel Álvarez-Rodríguez, Beatriz Martín-Fernández, Cristina Soriano-Úbeda, Felipe Martínez-Pastor
Chromatin status is critical for sperm fertility and reflects spermatogenic success. We tested a multivariate approach for studying pig sperm chromatin structure to capture its complexity with a set of quick and simple techniques, going beyond the usual assessment of DNA damage. Sperm doses from 36 boars (3 ejaculates/boar) were stored at 17 °C and analyzed on days 0 and 11. Analyses were: CASA (motility) and flow cytometry to assess sperm functionality and chromatin structure by SCSA (%DFI, DNA fragmentation; %HDS, chromatin maturity), monobromobimane (mBBr, tiol status/disulfide bridges between protamines), chromomycin A3 (CMA3, protamination), and 8-hydroxy-2'-deoxyguanosine (8-oxo-dG, DNA oxidative damage)...
February 6, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38370490/corrigendum-to-protective-effects-of-ceratonia-siliqua-extract-on-protamine-gene-expression-testicular-function-and-testicular-histology-in-doxorubicin-treated-adult-rats-an-experimental-study-int-j-reprod-biomed-2020-18-667-682
#13
Hengameh Mehdikhani, Mehrdad Shariati, Mohsen Forouzanfar, Seyed Ebrahim Hosseini
[This corrects the article DOI: 10.18502/ijrm.v13i8.7507.].
December 2023: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://read.qxmd.com/read/38369016/a-novel-sorting-method-for-the-enrichment-of-early-human-spermatocytes-from-clinical-biopsies
#14
JOURNAL ARTICLE
Meghan Robinson, Kevin Zhou, Sonia Hy Kung, Fatih Karaoğlanoğlu, Andrew Golin, Armita Safa, Charley Cai, Luke Witherspoon, Faraz Hach, Ryan Flannigan
OBJECTIVE: To determine if early spermatocytes can be enriched from a human testis biopsy using fluorescent-activated cell sorting (FACS). DESIGN: Potential surface markers for early spermatocytes were identified using bioinformatics analysis of single cell RNA sequenced (scRNAseq) human testis tissue. Testicular sperm extraction (TESE) samples from 3 participants with normal spermatogenesis were digested into single cell suspensions and cryopreserved. 2-4 million cells were obtained from each and sorted by FACS as separate biological replicates using antibodies for the identified surface markers...
February 16, 2024: F&S science
https://read.qxmd.com/read/38360131/coronary-artery-bypass-grafting-cabg-induces-pro-inflammatory-and-immunomodulatory-phenotype-of-platelets-in-the-absence-of-a-pro-aggregatory-state
#15
JOURNAL ARTICLE
Ehteramolsadat Hosseini, Javad Ahmadi, Faranak Kargar, Mehran Ghasemzadeh
BACKGROUND: Coronary artery bypass grafting (CABG) is considered a choice treatment for patients suffering from coronary artery disease (CAD). In the inflammatory milieu of cardiopulmonary bypass (CPB), systemic inflammatory response syndrome (SIRS) can induce a platelet pro-inflammatory state which could exacerbate post-CABG inflammatory status while affecting later hemostatic function in patients. Therefore centered on platelets, the study presented here tried to evaluate the pro-inflammatory and immunomodulatory profile of platelets as well as pro-aggregatory status during CABG...
February 13, 2024: Microvascular Research
https://read.qxmd.com/read/38357407/comparison-of-blood-concentration-and-weight-based-heparin-and-protamine-dosing-strategies-for-cardiopulmonary-bypass-a-systematic-review-and-meta-analysis
#16
REVIEW
Gavin Raner, Yirui Hu, Cody Trowbridge, Li Zhang, John Logan, Xianren Wu, Xiaopeng Zhang
BACKGROUND:  The conventional method of heparin and protamine management during cardiopulmonary bypass (CPB) is based on total body weight which fails to account for the heterogeneous response to heparin in each patient. On the other hand, the literature is inconclusive on whether individualized anticoagulation management based on real-time blood heparin concentration improves post-CBP outcomes. METHODS:  We searched databases of Medline, Excerpta Medica dataBASE (EMBASE), PubMed, Cumulative Index to Nursing and Allied Health Literature (CINHL), and Google Scholar, recruiting randomized controlled trials (RCTs) and prospective studies comparing the outcomes of dosing heparin and/or protamine based on measured heparin concentration versus patient's total body weight for CPB...
February 2024: Curēus
https://read.qxmd.com/read/38346284/the-sperm-nuclear-basic-proteins-of-the-sword-fern-polystichum-munitum
#17
JOURNAL ARTICLE
Juan Ausio, Alistair Knox, Bo-Hyun Kim, Elaine Humphrey, Brent Gowen, Naoki Minamino, Patrick von Aderkas
Sperm nuclear basic proteins (SNBPs) were isolated from extracted antheridia-rich male gametophytes raised from spores of the swordfern, Polystichum munitum. Electrophoretic (acetic acid-urea PAGE and SDS-PAGE) and chromatographic (rp-HPLC) characterization of the nuclear proteins exhibited the characteristics of the histone (H-type). In both types of gel electrophoresis, Histones H1, H2A, and H2B showed an altered electrophoretic mobility corresponding to that which is routinely observed for the histones in other plants...
February 12, 2024: Biochemistry and Cell Biology
https://read.qxmd.com/read/38336896/sperm-specific-histone-h1-in-highly-condensed-sperm-nucleus-of-sargassum-horneri
#18
JOURNAL ARTICLE
Yu Takeuchi, Shinya Sato, Chikako Nagasato, Taizo Motomura, Shujiro Okuda, Masahiro Kasahara, Fumio Takahashi, Shinya Yoshikawa
Spermatogenesis is one of the most dramatic changes in cell differentiation. Remarkable chromatin condensation of the nucleus is observed in animal, plant, and algal sperm. Sperm nuclear basic proteins (SNBPs), such as protamine and sperm-specific histone, are involved in chromatin condensation of the sperm nucleus. Among brown algae, sperm of the oogamous Fucales algae have a condensed nucleus. However, the existence of sperm-specific SNBPs in Fucales algae was unclear. Here, we identified linker histone (histone H1) proteins in the sperm and analyzed changes in their gene expression pattern during spermatogenesis in Sargassum horneri...
February 9, 2024: Scientific Reports
https://read.qxmd.com/read/38320369/ursonic-acid-attenuates-spermatogenesis-in-oligozoospermia-mice-through-inhibiting-ferroptosis
#19
JOURNAL ARTICLE
Jin Dong, Yanli Dong, Hanqing Chen, Taowen Ye, Guangtong Chen, Boyi Fan, Xiaorong Wang, Jianwu Shi, Chengniu Wang
Ursonic acid (UNA) is a natural pentacyclic triterpene found in some medicinal plants and foods. The reproductive protective effect of UNA was evaluated in a mouse model of oligozoospermia induced by busulfan (BUS) at 30 mg/kg b.w.. The mice were initially divided into groups with UNA concentrations of 10, 30, 50, 100 mg/kg. Subsequently, based on sperm parameters, the optimal concentration of 50 mg/kg was identified. As a control, an additional group was supplemented with ursolic acid at a concentration of 50 mg/kg...
February 3, 2024: Bioorganic Chemistry
https://read.qxmd.com/read/38313521/computational-approach-to-elucidating-insulin-protamine-binding-interactions-and-dynamics-in-insulin-nph-formulations
#20
JOURNAL ARTICLE
Ketan Kumar Rohilla, Manoj Kumar Pandey
Insulin NPH is an intermediate-acting insulin. Its protracted action profile is due to the formation of microcrystalline suspensions when insulin is complexed with a basic peptide protamine, zinc ion, and phenolic ligands. Despite advancements in analytical techniques, the binding epitope and binding mode of the protamine in the insulin-protamine complex are still unknown. In this study, we used bioinformatics tools such as molecular docking and molecular dynamics (MD) simulations to compute the binding sites and energetics of the insulin-protamine complex...
January 30, 2024: ACS Omega
keyword
keyword
120746
1
2
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.