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https://www.readbyqxmd.com/read/29033041/short-term-venoarterial-extracorporeal-membrane-oxygenation-for-massive-endobronchial-hemorrhage-after-pulmonary-endarterectomy
#1
Stefan Guth, Christoph B Wiedenroth, Marc Wollenschläger, Manuel Jonas Richter, Hossein A Ghofrani, Matthias Arlt, Eckhard Mayer
OBJECTIVES: Pulmonary endarterectomy (PEA) is the only curative treatment option for patients with chronic thromboembolic pulmonary hypertension. Massive endobronchial bleeding that precludes weaning from cardiopulmonary bypass is an often-fatal complication of PEA. The aim of this study was to determine whether short-term extracorporeal membrane oxygenation (ECMO) is a safe and feasible procedure in patients with severe endobronchial bleeding. METHODS: From January 2014 to December 2016, 396 patients (mean age 60 ± 18 years, 54...
September 20, 2017: Journal of Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/29032681/excited-state-proton-transfer-on-the-surface-of-a-therapeutic-protein-protamine
#2
Ankur A Awasthi, Prabhat K Singh
Proton transfer reactions on biosurfaces play an important role in a myriad of biological processes. Herein, Excited-state proton transfer (ESPT) reaction of 8-Hydroxypyranine-1, 3, 6-trisulfonate (HPTS) has been investigated in the presence of an important therapeutic protein, Protamine, using ground-state absorption, steady-state and detailed time-resolved emission measurements. HPTS forms a 1:1 complex with PrS with high association constant of 2.6 x 104 M-1. The binding of HPTS with Protamine leads to a significant modulation in the ground state prototropic equilibrium causing a downward shift of 1...
October 15, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29017779/targeted-inhibition-of-hantavirus-replication-and-intracranial-pathogenesis-by-a-chimeric-protein-delivered-sirna
#3
Jie Yang, Ji-Feng Sun, Ting-Ting Wang, Xiao-Hong Guo, Jun-Xia Wei, Lin-Tao Jia, An-Gang Yang
Hantavirus (HV) infection, which underlies hantavirus hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome, remains to be a severe clinical challenge. Here, we synthesized small interfering RNAs (siRNAs) that target the encoding sequences of HV strain 76-118, and validated their inhibitory role in virus replication in HV-infected monkey kidney Vero E6 cells. A chimeric protein, 3G1-Cκ-tP, consisting of a single-chain antibody fragment (3G1) against the HV surface envelop glycoprotein, the constant region of human immunoglobulin κ chain (Cκ), and truncated protamine (amino acids 8-29, tP), was further generated...
October 7, 2017: Antiviral Research
https://www.readbyqxmd.com/read/29017373/clinical-considerations-for-use-of-insulin-degludec-insulin-aspart-in-japanese-patients
#4
Takahisa Hirose, Takuya Awata, Yuiko Yamamoto, Mads Peter Hemmingsen
Co-formulation of basal and bolus insulin components provides a simpler regimen for patients with type 2 diabetes than separate basal-bolus treatment. However, conventional premixed insulin products include a suboptimal protaminated basal component that requires resuspension prior to injection. Insulin degludec/insulin aspart (IDegAsp) is the first soluble co-formulation of a basal insulin with an ultra-long duration of action (IDeg) and a rapid-acting bolus insulin (IAsp) in a single injection. Areas covered: In this review, the authors summarize findings from pre-clinical studies and the clinical trial program and provide guidance for the initiating and switching of IDegAsp in different patient populations...
October 11, 2017: Expert Opinion on Biological Therapy
https://www.readbyqxmd.com/read/28987395/abciximab-heparin-therapy-for-left-ventricular-assist-device-implantation-in-patients-with-heparin-induced-thrombocytopenia
#5
Chia-Ling Lee, Paolo C Colombo, Andrew Eisenberger, David Diuguid, Douglas L Jennings, Jiho Han, Michael P Salna, Koji Takeda, Paul A Kurlansky, Melana Yuzefpolskaya, Arthur R Garan, Yoshifumi Naka, Hiroo Takayama
BACKGROUND: Optimal anticoagulation strategy remains uncertain in patients with heparin-induced thrombocytopenia (HIT) and undergoing left ventricular assist device (LVAD) implantation. We describe our protocol of abciximab and heparin in these patients. METHODS: Our protocol is to administer abciximab, 0.25 mg/kg loading dose, followed by continuous infusion of 0.125 μg · kg(-1) · min(-1) throughout cardiopulmonary bypass. Full-dose heparin is then given with subsequent additional doses to maintain an activated clotting time of 400 seconds or longer...
October 4, 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/28985041/influence-of-the-abstinence-period-on-human-sperm-quality-analysis-of-2-458-semen-samples
#6
Vanessa A Comar, Claudia G Petersen, Ana L Mauri, Mariana Mattila, Laura D Vagnini, Adriana Renzi, Bruna Petersen, Andreia Nicoletti, Felipe Dieamant, Joao Batista A Oliveira, Ricardo L R Baruffi, José G Franco
OBJECTIVE: The aim of this study was to evaluate the influence of different periods of abstinence on conventional semen parameters as well as functional parameters in human semen, including mitochondrial function, chromatin packing and sperm DNA fragmentation. METHODS: We recruited a cohort of 2,458 men undergoing infertility investigation. Semen analyses were performed according to WHO guidelines/morphology-motile sperm organelle morphology examination/MSOME. For DNA integrity analysis, the percentages of DNA fragmentation (TUNEL), abnormal chromatin packaging/underprotamination (chromomycin A3/CMA3), abnormal mitochondrial membrane potential (MMP/MitoTracker Green), and apoptosis (annexin-V) were recorded...
October 6, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28982981/the-hippo-pathway-regulator-kibra-promotes-podocyte-injury-by-inhibiting-yap-signaling-and-disrupting-actin-cytoskeletal-dynamics
#7
Kristin Meliambro, Jenny S Wong, Justina Ray, Rhodora C Calizo, Sara Towne, Beatriz Cole, Fadi El Salem, Ronald E Gordon, Lewis Kaufman, John C He, Evren U Azeloglu, Kirk N Campbell
Kidney podocytes represent a key constituent of the glomerular filtration barrier. Identifying the molecular mechanisms of podocyte injury and survival is important for better understanding and management of kidney diseases. KIBRA (KIdney BRAin protein), an upstream regulator of the Hippo signaling pathway encoded by the Wwc1 gene, shares the pro-injury properties of its putative binding partner dendrin and antagonizes the pro-survival signaling of the downstream Hippo pathway effector YAP (Yes-associated protein) in Drosophila and MCF10A cells...
October 5, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28977892/polymorphisms-of-protamine-genes-contribute-to-male-infertility-susceptibility-in-the-chinese-han-population
#8
Weijun Jiang, Peiran Zhu, Jing Zhang, Qiuyue Wu, Weiwei Li, Shuaimei Liu, Mengxia Ni, Maomao Yu, Jin Cao, Yi Li, Yingxia Cui, Xinyi Xia
Protamine (PRM) plays important roles in the packaging of DNA within the sperm nucleus. To investigate the role of PRM1/2 and transition protein 1 (TNP1) polymorphisms in male infertility, 636 infertile men and 442 healthy individuals were recruited into this case-controlled study of the Chinese Han population, using MassARRAY technology to analyze genotypes. Our analysis showed that there were no significant differences between controls and infertile cases among the five single nucleotide polymorphisms identified in PRM1, PRM2 and TNP1 [rs737008 (G/A), rs2301365 (C/A), rs2070923 (C/A), rs1646022 (C/G) and rs62180545 (A/G)]...
September 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28974132/comparison-of-activated-clotting-times-measured-using-the-hemochron-jr-signature-and-medtronic-act-plus-during-cardiopulmonary-bypass-with-acute-normovolemic-haemodilution
#9
Jung Min Lee, Eun Young Park, Kyung Mi Kim, Jong Chan Won, Tack Koon Jung, Soo Kyung Lee
Objective This study compared the activated clotting time (ACT) measured using the Hemochron Jr. Signature (HACT) with the ACT measured using the Medtronic ACT Plus (MACT) during cardiopulmonary bypass (CPB) with acute normovolemic haemodilution (ANH) in patients undergoing cardiac surgery. Methods The ACT was checked at baseline with both devices after inducing anaesthesia, and 400 to 800 mL of whole blood was withdrawn to induce moderate ANH. Before initiating CPB, a 300-IU/kg bolus dose of heparin was administered to maintain the HACT at >400 s; protamine was later given to reverse the anticoagulation...
January 1, 2017: Journal of International Medical Research
https://www.readbyqxmd.com/read/28974116/decreased-reproductive-performance-in-xct-knockout-male-mice
#10
Shinji Hamashima, Takujiro Homma, Sho Kobayashi, Naoki Ishii, Toshihiro Kurahashi, Ren Watanabe, Naoko Kimura, Hideyo Sato, Junichi Fujii
Sulfoxidation occurs in protamines that are enriched in cysteine and supplies chromatin for packaging. The extracellular fluid contains higher levels of oxidized cysteine (cystine), and some cells utilize system xc(-), a cystine transporter in which xCT is the main protein component, to fulfill the need for cysteine. We hypothesized that system xc(-) might ensure the supply of cysteine needed for spermatogenesis. The reproductive ability of xCT(-/-) male mice at 6- to 18-weeks of age appeared to be lower than xCT(+/+) male mice...
October 4, 2017: Free Radical Research
https://www.readbyqxmd.com/read/28969455/the-impact-of-cigarette-smoking-on-protamines-1-and-2-transcripts-in-human-spermatozoa
#11
Mohammed Hamad, Nyaz Shelko, Mathias Montenarh, Mohammed Eid Hammadeh
This study was conducted to evaluate the relation between cigarette smoking, semen quality and ratios of protamine mRNAs in smokers and non-smokers. Spermatozoa from 123 men and 64 smokers and 59 non-smokers whose female partners attended an assisted reproduction and andrology laboratory were evaluated. Protamine mRNA was extracted from purified sperm, reverse-transcribed and subjected to real-time quantitative PCR using specific primer pairs for protamine 1 (PRM1) and protamine 2 (PRM2). The main outcomes showed that PRM1 mRNA levels in smokers were significantly lower (p = 0...
October 2, 2017: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/28960085/the-clinical-effectiveness-of-insulin-glargine-in-patients-with-type-i-diabetes-in-brazil-findings-and-implications
#12
Lays P Marra, Vânia E Araújo, Gerusa Cc Oliveira, Leonardo M Diniz, Augusto A Guerra Júnior, Francisco de Assis Acurcio, Brian Godman, Juliana Álvares
AIM: Published studies have challenged the cost-effectiveness of insulin glargine versus neutral protamine hagedorn (NPH) insulins in Brazil with limited evidence of increased effectiveness despite considerably higher acquisition costs. However, still a controversy. Consequently, there is a need to address this. MATERIALS & METHODS: Retrospective cohort study of Type I diabetes patients receiving insulin glargine in Brazil following NPH insulin who met the criteria...
September 29, 2017: Journal of Comparative Effectiveness Research
https://www.readbyqxmd.com/read/28953430/long-term-use-of-long-acting-insulin-analogs-and-breast-cancer-incidence-in-women-with-type-2-diabetes
#13
Jennifer W Wu, Laurent Azoulay, Agnieszka Majdan, Jean-François Boivin, Michael Pollak, Samy Suissa
Purpose The association between long-acting insulin analogs and increased breast cancer risk is uncertain, particularly with the short follow-up in previous studies. We assessed this risk long term in women with type 2 diabetes. Methods A population-based cohort of women 40 years or older, all of whom were treated with long-acting (glargine, detemir) or neutral protamine Hagedorn (NPH) insulin between 2002 and 2012, was formed using the United Kingdom's Clinical Practice Research Datalink. Women were followed until February 2015 or breast cancer diagnosis...
September 27, 2017: Journal of Clinical Oncology: Official Journal of the American Society of Clinical Oncology
https://www.readbyqxmd.com/read/28951354/-construction-and-verification-of-anti-mm-scfv-tp-fusion-protein-expression-vector
#14
Hao Wang, Yi-Fei Yang, Wei Wang, Bing Guan, Meng Xun, Hai Zhang, Zi-Ling Wang, Yong Zhao
OBJECTIVE: To construct an expression vector of anti-MM scFv-tP fusion protein and test its expression efficiency and function. METHODS: The truncated protamine (tP) gene sequence was added to the gene of single chain antibody against the specific antigen on the surface of malignant melanoma tumor cells using PCR. A GST-fusion expression vector was constructed and the soluable protein was expressed in the E.coli system. After cleavage and purification, the purified fusion protein was obtained...
September 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28949791/estrogen-through-estrogen-receptor-1-regulates-histone-modifications-and-chromatin-remodeling-during-spermatogenesis-in-adult-rats
#15
Kushaan Dumasia, Anita Kumar, Sharvari Deshpande, Nafisa H Balasinor
Estrogen receptors (ESR1 and ESR2) play crucial roles in various processes during spermatogenesis. To elucidate individual roles of ESRs in male fertility, we developed in vivo selective ESR agonist administration models. Adult male rats treated with ESR1 and ESR2 agonist for 60 days show spermatogenic defects leading to reduced sperm counts and fertility. While studying epigenetic changes in the male germ line that could have affected fertility, we earlier observed a decrease in DNA methylation and its machinery upon ESR2 agonist treatment...
September 26, 2017: Epigenetics: Official Journal of the DNA Methylation Society
https://www.readbyqxmd.com/read/28949131/heparin-binding-to-an-engineered-virus-like-nanoparticle-antagonist
#16
Ho Yong Cheong, Myles Groner, Kevin Hong, Brennen Arthur Lynch, William R Hollingsworth, Zinaida Polonskaya, Jin-Kyu Rhee, Michael M Baksh, M G Finn, Andrew J Gale, Andrew K Udit
The anti-coagulant activity of heparin administered during medical interventions must be reversed to restore normal clotting, typically by titrating with protamine. Given the acute toxicity associated with protamine, we endeavored to generate safer heparin antagonists by engineering bacteriophage Qβ virus-like particles (VLPs) to display motifs that bind heparin. A particle bearing a single amino acid change from wild-type (T18R) was identified as a promising candidate for heparin antagonism. Surface potential maps generated through molecular modeling reveal that the T18R mutation adds synergistically to adjacent positive charges on the particle surface, resulting in a large solvent-accessible cationic region that is replicated 180 times over the capsid...
September 26, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28944659/t2-weighted-magnetic-resonance-imaging-of-hepatic-tumor-guided-by-spio-loaded-nanostructured-lipid-carriers-and-ferritin-reporter-genes
#17
Chen-Ying Lu, Jian-Song Ji, Xiu-Liang Zhu, Pei-Feng Tang, Qian Zhang, Nan-Nan Zhang, Zu-Hua Wang, Xiao-Juan Wang, Wei-Qian Chen, Jing-Bo Hu, Yong-Zhong Du, Ri-Sheng Yu
Nowadays, there is a high demand for supersensitive contrast agents for the early diagnostics of hepatocarcinoma. It has been recognized that accurate imaging information is able to be achieved by constructing hepatic tumor specific targeting probes, though it still faces challenges. Here, a AGKGTPSLETTP peptide (A54)-functionalized superparamagnetic iron oxide (SPIO)-loaded nanostructured lipid carrier (A54-SNLC), which can be specifically uptaken by hepatoma carcinoma cell (Bel-7402) and exhibited ultralow imaging signal intensity with varied Fe concentration on T2-weighted imaging (T2WI), was first prepared as an effective gene carrier...
October 4, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28935443/complex-formation-with-protamine-prolongs-the-thrombin-inhibiting-effect-of-dna-aptamer-in%C3%A2-vivo
#18
V A Spiridonova, T M Novikova, D M Nikulina, T A Shishkina, E V Golubkina, O S Dyukareva, N N Trizno
Antithrombin DNA aptamersRE31 are single-chain oligonucleotides that fold into three-dimensional forms allowing them to bind the enzyme with high affinity and inhibit its activity in vivo. They are rapidly degraded by a nonspecific nuclease, and, to prolong the lifetime of the aptamer DNA in the bloodstream, it is necessary to coat it with a polymer envelope. A new approach to solving this problem based on preparation of DNA-polyelectrolyte complexes with a minimal particle size that can circulate with blood flow...
September 19, 2017: Biochimie
https://www.readbyqxmd.com/read/28930672/histone-h3-methylated-at-arginine-17-is-essential-for-reprogramming-the-paternal-genome-in-zygotes
#19
Yuki Hatanaka, Takeshi Tsusaka, Natsumi Shimizu, Kohtaro Morita, Takehiro Suzuki, Shinichi Machida, Manabu Satoh, Arata Honda, Michiko Hirose, Satoshi Kamimura, Narumi Ogonuki, Toshinobu Nakamura, Kimiko Inoue, Yoshihiko Hosoi, Naoshi Dohmae, Toru Nakano, Hitoshi Kurumizaka, Kazuya Matsumoto, Yoichi Shinkai, Atsuo Ogura
At fertilization, the paternal genome undergoes extensive reprogramming through protamine-histone exchange and active DNA demethylation, but only a few maternal factors have been defined in these processes. We identified maternal Mettl23 as a protein arginine methyltransferase (PRMT), which most likely catalyzes the asymmetric dimethylation of histone H3R17 (H3R17me2a), as indicated by in vitro assays and treatment with TBBD, an H3R17 PRMT inhibitor. Maternal histone H3.3, which is essential for paternal nucleosomal assembly, is unable to be incorporated into the male pronucleus when it lacks R17me2a...
September 19, 2017: Cell Reports
https://www.readbyqxmd.com/read/28885731/a-multifunctional-nanocarrier-for-efficient-trail-based-gene-therapy-against-hepatocellular-carcinoma-with-desmoplasia
#20
Chun-Hung Liu, Guann-Gen Chern, Fu-Fei Hsu, Kuan-Wei Huang, Yun-Chieh Sung, Hsi-Chien Huang, Jiantai Timothy Qiu, Sheng-Kai Wang, Chu-Chi Lin, Chien-Hsun Wu, Han-Chung Wu, Jia-Yu Liu, Yunching Chen
The anti-cancer efficacy of TNF-related apoptosis-inducing ligand (TRAIL)-based therapy is limited due to systemic toxicity, poor bioavailability, and the development of TRAIL-resistance. We developed a tumor-targeted LCPP (Lipid/Calcium/Phosphate/Protamine) nanoparticle (NP) to deliver TRAIL plasmid DNA (pDNA) into hepatocellular carcinoma (HCC) cells. TRAIL pDNA was encapsulated in a pH stimuli-responsive calcium phosphate (CaP) core, and protamine was added to facilitate nuclear delivery of pDNA. In addition, the intracellular release of Ca(2+) from the CaP core overcame TRAIL resistance via calcium influx-dependent DR5 upregulation...
September 8, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
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