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https://www.readbyqxmd.com/read/29449095/relationships-between-bacteriospermia-dna-integrity-nuclear-protamine-alteration-sperm-quality-and-icsi-outcome
#1
Ali Zeyad, Mohammed Hamad, Houda Amor, Mohamad Eid Hammadeh
The Aim of this study was to evaluate the effects of bacteriospermia on human sperm parameters, nuclear protamines, DNA integrity and ICSI outcome in patients enrolled for ICSI treatment. 84 unselected couples consulting in infertility and obstetrics clinic and enrolled for ICSI treatment were included in this study. The semen specimens were screened bacteriologically; semen and sperm parameters were also evaluated according to WHO guidelines. DNA integrity, protamines concentration and protamine deficiency were estimated by TUNEL assay, AU-PAGE and Chromomycin (CMA3) respectively...
February 12, 2018: Reproductive Biology
https://www.readbyqxmd.com/read/29448511/preparation-and-in-vitro-evaluation-of-elastic-nanoliposomes-for-topical-delivery-of-highly-skin-permeable-growth-factors
#2
Sang Won Choi, Min-Ho Oak, Eunyoung Yi, Hyun Jung Kim, Jin Woo Park
Percutaneous delivery of growth factors is often used to treat wounds, and for cosmetic purposes, as a way of accelerating healing and skin regeneration, respectively. However, the therapeutic effects of growth factors are diminished by their poor absorption when delivered percutaneously, in addition to their rapid degradation by proteinases. To overcome these obstacles, we constructed two skin-permeable compounds. Basic fibroblast growth factor (bFGF) and vascular endothelial growth factor-A (VEGF-A) were both genetically paired with low-molecular-weight protamine (LMWP), to yield the compounds LMWP-bFGF and LMWP-VEGF-A, respectively...
February 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29448503/construction-and-characterization-of-cell-penetrating-peptide-fused-fibroblast-growth-factor-and-vascular-endothelial-growth-factor-for-an-enhanced-percutaneous-delivery-system
#3
Sang Won Choi, Rudra Pangeni, Dai Hyun Jung, Su Jung Kim, Jin Woo Park
The exogenous administration of growth factors has been examined for treating wounds and such factors serve as cosmetic agents for skin regeneration. However, the topical application of growth factors is hampered by their limited percutaneous absorption. In this study, we genetically modified basic fibroblast growth factor (bFGF) and vascular endothelial growth factor-A (VEGF-A) using a cell-penetrating peptide to facilitate their permeation into skin and to avoid multiple steps in the chemical or physical modification of biomaterials...
February 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29436217/a-stealth-and-fully-laden-drug-carrier-self-assembled-nanogel-encapsulated-with-epigallocatechin-gallate-and-sirna-for-drug-resistant-breast-cancer-therapy
#4
Jie Ding, Tingxizi Liang, Qianhao Min, Li-Ping Jiang, Jun-Jie Zhu
For co-delivery of therapeutic genes and chemical agents in combined therapy, the ideal drug delivery system (DDS) entails high-capacity and low-body toxicity carriers, allowing adequate drug dose for tumor region while yielding low residue in normal tissue. To augment the gene/drug load capacity and circumvent the potential toxicity brought by traditional inorganic and polymeric nanocarriers, a "stealth" carrier was herein designed in a simple self-assembly of (-)-epigallocatechin-3-O-gallate (EGCG) and small interfering RNA (siRNA) by recruiting protamine as biodegradable medium for the treatment of drug-resistant triple-negative breast cancer (TNBC)...
February 13, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29430275/engineering-a-simple-lateral-flow-device-for-animal-blood-coagulation-monitoring
#5
Hua Li, Daewoo Han, Giovanni M Pauletti, Andrew J Steckl
Increasing numbers of animals are diagnosed with thromboembolism, requiring anticoagulation treatment to prevent thrombotic events. Frequent and periodic coagulation monitoring is critical to ensure treatment effectiveness and patient safety by limiting blood coagulation ability within the desired therapeutic range. Point-of-care diagnostics is an ideal candidate for frequent coagulation monitoring due to rapid test results and no need for laboratory setting. This article reports the first utilization of no-reaction lateral flow assay (nrLFA) device for simple and low-cost animal blood coagulation monitoring in resource-limited setting...
January 2018: Biomicrofluidics
https://www.readbyqxmd.com/read/29421488/comparison-of-the-pharmacodynamics-of-protamine-zinc-insulin-and-insulin-degludec-and-validation-of-the-continuous-glucose-monitoring-system-ipro2-in-healthy-cats
#6
Elena Salesov, Eric Zini, Angelina Riederer, Thomas A Lutz, Claudia E Reusch
With the aim to improve current therapeutic and monitoring options for diabetic cats, the present study compared pharmacodynamic parameters of protamine zinc insulin (PZI) and insulin degludec and validated the continuous glucose monitoring system (CGMS) iPro2 with Sof-sensor and Enlite-sensor focusing on the low glycemic range. Three doses (0.1, 0.2 and 0.3IU/kg) of the two insulin preparations and the CGMS iPro2 with two different sensors were tested in six healthy cats. After each insulin administration, onset of action, time to glucose nadir and duration of action were calculated by measuring glucose concentrations with a portable blood glucose meter (PBGM)...
January 26, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29403004/a-new-strategy-for-the-passive-skin-delivery-of-nanoparticulate-high-molecular-weight-hyaluronic-acid-prepared-by-a-polyion-complex-method
#7
Yoshihiro Tokudome, Tatsuya Komi, Ami Omata, Marie Sekita
Restoring hyaluronic acid (HA) content is important for maintaining the function of photo-aged skin. This study aimed to evaluate the passive delivery into skin of HA nanoparticles formed by the polyion complex method. Nanoparticles were prepared by mixing and stirring anionic HA with a cationic polymer, protamine, at the charge ratio 55:45. The permeation of fluorescently-labelled HA nanoparticles (HANP) or free HA through hairless mouse skin was characterized in vitro. HANP or free HA was applied to ultraviolet (UV)-irradiated mice in vivo, and their transepidermal water loss (TEWL) was measured after 4 days...
February 5, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29401728/antibacterial-effects-of-chitosan-cationic-peptide-nanoparticles
#8
Frans Ricardo Tamara, Chi Lin, Fwu-Long Mi, Yi-Cheng Ho
This study attempted to develop chitosan-based nanoparticles with increased stability and antibacterial activity. The chitosan/protamine hybrid nanoparticles were formed based on an ionic gelation method by mixing chitosan with protamine and subsequently cross-linking the mixtures with sodium tripolyphosphate (TPP). The effects of protamine on the chemical structures, physical properties, and antibacterial activities of the hybrid nanoparticles were investigated. The antibacterial experiments demonstrated that the addition of protamine (125 µg/mL) in the hybrid nanoparticles (500 µg/mL chitosan and 166...
February 5, 2018: Nanomaterials
https://www.readbyqxmd.com/read/29370526/nanoparticle-mediated-trapping-of-wnt-family-member-5a-in-tumor-microenvironments-enhances-immunotherapy-for-b-raf-proto-oncogene-mutant-melanoma
#9
Qi Liu, Hongda Zhu, Karthik Tiruthani, Limei Shen, Fengqian Chen, Keliang Gao, Xueqiong Zhang, Lin Hou, Degeng Wang, Rihe Liu, Leaf Huang
Development of an effective treatment against advanced tumors remains a major challenge for cancer immunotherapy. Approximately 50% of human melanoma is driven by B-Raf proto-oncogene mutation (BRAF-mutant). Tumors with such mutation are desmoplastic, highly immunosuppressive, and often resistant to immune checkpoint therapies. We have shown that immunotherapy mediated by low dose doxorubicin-induced immunogenic cell death was only partially effective for this type of tumor, and not effective in long-term inhibition of tumor progression...
January 25, 2018: ACS Nano
https://www.readbyqxmd.com/read/29368965/consensus-statement-by-the-american-association-of-clinical-endocrinologists-and-american-college-of-endocrinology-on-the-comprehensive-type-2-diabetes-management-algorithm-2018-executive-summary
#10
Alan J Garber, Martin J Abrahamson, Joshua I Barzilay, Lawrence Blonde, Zachary T Bloomgarden, Michael A Bush, Samuel Dagogo-Jack, Ralph A DeFronzo, Daniel Einhorn, Vivian A Fonseca, Jeffrey R Garber, W Timothy Garvey, George Grunberger, Yehuda Handelsman, Irl B Hirsch, Paul S Jellinger, Janet B McGill, Jeffrey I Mechanick, Paul D Rosenblit, Guillermo E Umpierrez
A1C = hemoglobin A1C; AACE = American Association of Clinical Endocrinologists; ACCORD = Action to Control Cardiovascular Risk in Diabetes; ACCORD BP = Action to Control Cardiovascular Risk in Diabetes Blood Pressure; ACEI = angiotensin-converting enzyme inhibitor; ADVANCE = Action in Diabetes and Vascular Disease: Preterax and Diamicron MR Controlled Evaluation; AGI = alpha-glucosidase inhibitor; apo B = apolipoprotein B; ASCVD = atherosclerotic cardiovascular disease; BAS = bile acid sequestrant; BCR-QR = bromocriptine quick release; BMI = body mass index; BP = blood pressure; CCB = calcium channel blocker; CHD = coronary heart disease; CKD = chronic kidney disease; CVD = cardiovascular disease; DASH = Dietary Approaches to Stop Hypertension; DPP4 = dipeptidyl peptidase 4; eGFR = estimated glomerular filtration rate; ER = extended release; FDA = Food and Drug Administration; GLP1 = glucagon-like peptide 1; HDL-C = high-density lipoprotein cholesterol; IMPROVE-IT = Improved Reduction of Outcomes: Vytorin Efficacy International Trial; LDL-C = low-density lipoprotein cholesterol; LDL-P = low-density lipoprotein particle; Look AHEAD = Look Action for Health in Diabetes; NPH = neutral protamine Hagedorn; OSA = obstructive sleep apnea; RCT = randomized controlled trial; SU = sulfonylurea; SGLT2 = sodium glucose cotransporter-2; SMBG = self-monitoring of blood glucose; T2D = type 2 diabetes; TZD = thiazolidinedione; VADT = Veterans Affairs Diabetes Trial...
January 2018: Endocrine Practice
https://www.readbyqxmd.com/read/29363751/inactivated-antithombin-as-anticoagulant-reversal-in-a-rat-model-of-cardiopulmonary-bypass-a-potent-and-potentially-safer-alternative-to-protamine
#11
Elsa P Bianchini, Alexandre Sebestyen, Toufik Abache, Yasmine Bourti, Alexandre Fontayne, Vincent Richard, Fabienne Tamion, Jean-Luc Plantier, Fabien Doguet, Delphine Borgel
Heparin anticoagulation followed by protamine reversal is commonly used in cardiopulmonary bypass (CPB). As an alternative to protamine, a recombinant inactive antithrombin (riAT) was designed as an antidote to heparin and was previously shown to be as potent as protamine in-vitro. In the present study, riAT was assessed for its ability to neutralize heparin after CPB in a rat model. After 60 min of CPB under heparin, rats received 5 mg/kg protamine, 37.5 mg/kg riAT or phosphate buffered saline (PBS) as placebo...
January 24, 2018: British Journal of Haematology
https://www.readbyqxmd.com/read/29349578/in-situ-tissue-labeling-of-cerebral-amyloid-using-hiv-related-tat-peptide
#12
E Maderna, L Colombo, A Cagnotto, G Di Fede, A Indaco, F Tagliavini, M Salmona, G Giaccone
Delivering peptide-based drugs to the brain is a major challenge because of the existence of the blood-brain barrier (BBB). To overcome this problem, cell-penetrating peptides derived from proteins that are able to cross biological membranes have been used as cell-permeable and brain-penetrant compounds. An example is the transactivator of transcription protein transduction domain (Tat) of the human immunodeficiency virus. The basic domain of Tat is formed of arginine and lysine amino acid residues. Tat has been used as brain-penetrant carrier also in therapies for Alzheimer disease (AD), the most common form of dementia characterized by extracellular cerebral deposits of amyloid made up of Aβ peptide...
January 19, 2018: Molecular Neurobiology
https://www.readbyqxmd.com/read/29322308/quality-of-life-of-patients-with-type-1-diabetes-mellitus-using-insulin-analog-glargine-compared-with-nph-insulin-a-systematic-review-and-policy-implications
#13
REVIEW
Paulo H R F Almeida, Thales B C Silva, Francisco de Assis Acurcio, Augusto A Guerra Júnior, Vania E Araújo, Leonardo M Diniz, Brian Godman, Alessandra M Almeida, Juliana Alvares
INTRODUCTION: Insulin analog glargine (GLA) has been available as one of the therapeutic options for patients with type 1 diabetes mellitus to enhance glycemic control. Studies have shown that a decrease in the frequency of hypoglycemic episodes improves the quality of life (QoL) of diabetic patients. However, there are appreciable acquisition cost differences between different insulins. Consequently, there is a need to assess their impact on QoL to provide future guidance to health authorities...
January 10, 2018: Patient
https://www.readbyqxmd.com/read/29302118/blood-and-blood-product-conservation-results-of-strategies-to-improve-clinical-outcomes-in-open-heart-surgery-patients-at-a-tertiary-hospital
#14
Junaid H Khan, Emily A Green, Jimmin Chang, Alexandria M Ayala, Marilyn S Barkin, Emily E Reinys, Jeffrey Stanton, Russell D Stanten
Blood product usage is a quality outcome for patients undergoing cardiac surgery. To address an increase in blood product usage since the discontinuation of aprotinin, blood conservation strategies were initiated at a tertiary hospital in Oakland, CA. Improving transfusion rates for open heart surgery patients requiring Cardiopulmonary bypass (CPB) involved multiple departments in coordination. Specific changes to conserve blood product usage included advanced CPB technology upgrades, and precise individualized heparin dose response titration assay for heparin and protamine management...
December 2017: Journal of Extra-corporeal Technology
https://www.readbyqxmd.com/read/29296706/serologic-characterization-of-anti-protamine-heparin-and-anti-pf4-heparin-antibodies
#15
Grace M Lee, Manali Joglekar, Maragatha Kuchibhatla, Sanjay Khandelwal, Rui Qi, Lubica Rauova, Gowthami M Arepally
Anti-protamine (PRT)/heparin antibodies are a newly described class of heparin-dependent antibodies occurring in patients exposed to PRT and heparin during cardiac surgery. To understand the biologic significance of anti-PRT/heparin antibodies, we developed a murine monoclonal antibody (ADA) specific for PRT/heparin complexes and compared it to patient-derived anti-PRT/heparin antibodies, as well as comparing polyclonal and monoclonal antibodies with anti-platelet factor 4 (PF4)/heparin. Using monoclonal antibodies and polyclonal patient-derived antibodies, we show distinctive binding patterns of anti-PRT/heparin antibodies as compared with PF4/heparin antibodies...
April 25, 2017: Blood Advances
https://www.readbyqxmd.com/read/29290078/is-300-seconds-act-safe-and-efficient-during-miecc-procedures
#16
Adrian Bauer, Harald Hausmann, Jan Schaarschmidt, Michal Szlapka, Martin Scharpenberg, Thomas Eberle, J Michael Hasenkam
INTRODUCTION:  The recommended minimum activated clotting time (ACT) level for cardiopulmonary bypass (CPB) of 480 seconds originated from investigations with bubble oxygenators and uncoated extracorporeal circulation (ECC) systems. Modern minimal invasive ECC (MiECC) systems are completely closed circuits containing a membrane oxygenator and a tip-to-tip surface coating. We hypothesized that surface coating and the "closed-loop" design allow the MiECC to safely run with lower ACT levels and that an ACT level of 300 seconds can be safely applied without thromboembolic complications...
December 31, 2017: Thoracic and Cardiovascular Surgeon
https://www.readbyqxmd.com/read/29289359/safety-of-uninterrupted-periprocedural-edoxaban-versus-phenprocoumon-for-patients-who-underwent-left-atrial-catheter-ablation-procedures
#17
Marc Kottmaier, Felix Bourier, Hannah Pausch, Tilko Reents, Verena Semmler, Martha Telishevska, Katharina Koch-Büttner, Carsten Lennerz, Sarah Lengauer, Marielouise Kornmayer, Elena Rousseva, Stephanie Brooks, Amir Brkic, Sonia Ammar-Busch, Bernhard Kaess, Roger Dillier, Christian Grebmer, Christoph Kolb, Gabriele Hessling, Isabel Deisenhofer
Data about the safety of edoxaban in patients who underwent left atrial (LA) radiofrequency (RF) ablation procedures are lacking. This study sought to compare the safety of uninterrupted edoxaban with uninterrupted phenprocoumon administration during LA RF ablation for atrial fibrillation and atrial tachycardia. In total, 231 patients (mean age 64 ± 11years, male 71%) who underwent LA RF ablation under continuous oral anticoagulation (OAC) with edoxaban or phenprocoumon were included in the study. Patients on uninterrupted edoxaban (60 mg or 30 mg/day for at least 4 weeks) were matched for gender, age and type of arrhythmia with 2 patients on uninterrupted phenprocoumon (international normalized ratio 2 to 3)...
November 23, 2017: American Journal of Cardiology
https://www.readbyqxmd.com/read/29277682/aptamer-guided-sirna-loaded-nanomedicines-for-systemic-gene-silencing-in-cd-44-expressing-murine-triple-negative-breast-cancer-model
#18
Walhan Alshaer, Hervé Hillaireau, Juliette Vergnaud, Simona Mura, Claudine Delomenie, Félix Sauvage, Said Ismail, Elias Fattal
In this study, we describe a liposomal-based siRNA delivery system with a core composed of siRNA:protamine complex and a shell designed for the active targeting of CD44-expressing cells using for the first time the anti-CD44 aptamer (named Apt1) as targeting ligand. Among all functions, CD44 is the most common cancer stem cell surface biomarker and is found overexpressed in many tumors making this an attractive receptor for therapeutic targeting. This unique non-cationic system was evaluated for the silencing of the reporter gene of luciferase (luc2) in a triple-negative breast cancer model in vitro and in vivo...
December 22, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/29276400/challenges-and-unmet-needs-in-basal-insulin-therapy-lessons-from-the-asian-experience
#19
REVIEW
Wing Bun Chan, Jung Fu Chen, Su-Yen Goh, Thi Thanh Huyen Vu, Iris Thiele Isip-Tan, Sony Wibisono Mudjanarko, Shailendra Bajpai, Maria Aileen Mabunay, Pongamorn Bunnag
Basal insulin therapy can improve glycemic control in people with type 2 diabetes. However, timely initiation, optimal titration, and proper adherence to prescribed basal insulin regimens are necessary to achieve optimal glycemic control. Even so, glycemic control may remain suboptimal in a significant proportion of patients. Unique circumstances in Asia (eg, limited resources, management of diabetes primarily in nonspecialist settings, and patient populations that are predominantly less educated) coupled with the limitations of current basal insulin options (eg, risk of hypoglycemia and dosing time inflexibility) amplify the challenge of optimal basal insulin therapy in Asia...
2017: Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy
https://www.readbyqxmd.com/read/29241887/basal-insulin-regimens-for-adults-with-type-1-diabetes-mellitus-a-cost-utility-analysis
#20
Dalia Dawoud, Elisabetta Fenu, Bernard Higgins, David Wonderling, Stephanie A Amiel
OBJECTIVES: To assess the cost-effectiveness of basal insulin regimens for adults with type 1 diabetes mellitus in England. METHODS: A cost-utility analysis was conducted in accordance with the National Institute for Health and Care Excellence reference case. The UK National Health Service and personal and social services perspective was used and a 3.5% discount rate was applied for both costs and outcomes. Relative effectiveness estimates were based on a systematic review of published trials and a Bayesian network meta-analysis...
December 2017: Value in Health: the Journal of the International Society for Pharmacoeconomics and Outcomes Research
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