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https://www.readbyqxmd.com/read/28645649/evaluation-of-a-selective-chromogenic-medium-for-detecting-vancomycin-resistant-enterococci
#1
Renata Oliveira Soares, Adriana Medianeira Rossato, Gustavo Enck Sambrano, Neidimar Cezar Corrêa Tolfo, Juliana Caierão, Thiago Galvão da Silva Paim, Pedro Alves d'Azevedo
Rapid identification of vancomycin-resistant enterococci (VRE) can assist in choosing the appropriate treatment and preventing VRE spread. The performance of chromID™ VRE agar was evaluated using 184 clinical isolates of Enterococcus spp. and reference strains. The test had a sensitivity of 95.52% but a low specificity of 30%.
June 8, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28641915/the-antimicrobial-spectrum-of-xeroform-%C3%A2
#2
David J Barillo, Anthony R Barillo, Sarah Korn, Kan Lam, Paul S Attar
INTRODUCTION/BACKGROUND: Xeroform(®) is a petrolatum-based fine mesh gauze containing 3% bismuth tribromophenate. Bismuth, similar to other metals, has antimicrobial properties. Xeroform(®) has been used for decades in burn and plastic surgery as a donor site dressing and as a covering for wounds or partial thickness burns. Despite this, the antimicrobial spectrum of Xeroform(®) remains largely unknown. We examined the in-vitro efficacy of Xeroform(®) against common burn pathogens using zone-of-inhibition methodology in a commercial research facility...
June 19, 2017: Burns: Journal of the International Society for Burn Injuries
https://www.readbyqxmd.com/read/28627467/time-to-review-the-gold-standard-for-genotyping-vancomycin-resistant-enterococci-in-epidemiology-comparing-whole-genome-sequencing-with-pfge-and-mlst-in-three-suspected-outbreaks-in-sweden-during-2013-2015
#3
Birgitta Lytsy, Lars Engstrand, Åke Gustafsson, Rene Kaden
Vancomycin-resistant enterococci (VRE) are a challenge to the health-care system regarding transmission rate and treatment of infections. VRE outbreaks have to be controlled from the first cases which means that appropriate and sensitive genotyping methods are needed. The aim of this study was to investigate the applicability of whole genome sequencing based analysis compared to Pulsed-Field Gel Electrophoresis (PFGE) and Multi-Locus Sequence Typing (MLST) in epidemiological investigations as well as the development of a user friendly method for daily laboratory use...
June 15, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28622949/changed-epidemiology-during-intra-and-interhospital-spread-of-high-risk-clones-of-vana-containing-enterococcus-in-brazilian-hospitals
#4
Andrey G Sacramento, Rosemeire C Zanella, Fernanda Esposito, Emanuela A S Costa, Lara M de Almeida, Carlos Pires, Artemir C de Brito, Elsa M Mamizuka, Louise T Cerdeira, Nilton Lincopan
We report changes in the molecular epidemiology of vanA-containing Enterococcus during the intra and interhospital spread of high-risk clones, in Southeastern Brazil. While VRE faecalis predominated during 1998 to 2006, a reversal has been observed in the last years, where VRE faecium belonging to ST114, ST203, ST412, ST478 and ST858 have become endemic.
May 18, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28617344/synthesis-and-biological-activity-evaluation-of-novel-heterocyclic-pleuromutilin-derivatives
#5
Yunpeng Yi, Yunxing Fu, Pengcheng Dong, Wenwen Qin, Yu Liu, Jiangping Liang, Ruofeng Shang
A series of pleuromutilin derivatives were synthesized by two synthetic procedures under mild reaction conditions and characterized by Nuclear Magnetic Resonance (NMR), Infrared Spectroscopy (IR), and High Resolution Mass Spectrometer (HRMS). Most of the derivatives with heterocyclic groups at the C-14 side of pleuromutilin exhibited excellent in vitro antibacterial activities against Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), methicillin-resistant Staphylococcus epidermidis (MRSE), and vancomycin-resistant Enterococcus (VRE) in vitro antibacterial activity...
June 15, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28586174/2-6-difluorobenzamide-inhibitors-of-the-bacterial-cell-division-protein-ftsz-design-synthesis-and-structure-activity-relationship-study
#6
Valentina Straniero, Ermanno Valoti, Carlo Zanotto, Letizia Straniero, Andrea Casiraghi, Stefano Duga, Antonia Radaelli, Carlo de Giuli Morghen
A wide variety of drug-resistant microorganism are continuously emerging, restricting the therapy of common bacterial infections. Originally potent antimicrobial agents are now no longer helpful, due to their weak or null activity towards these antibiotic-resistant bacteria. In addition, none of the recently approved antibiotics affects innovative targets, resulting in a requirement of novel drugs, with innovative antibacterial mechanisms of action. The essential cell division protein FtsZ (Filamentous temperature sensitive Z) emerged as a possible target, thanks to its ubiquitous expression and its homology to eukaryotic -tubulin...
June 6, 2017: ChemMedChem
https://www.readbyqxmd.com/read/28578090/genotyping-antimicrobial-resistance-and-virulence-factor-gene-profiles-of-vancomycin-resistance-enterococcus-faecalis-isolated-from-blood-culture
#7
Shahnaz Armin, Fatemeh Fallah, Abdollah Karimi, Marjan Rashidan, Mehdi Shirdust, Leila Azimi
BACKGROUND: Enterococcus spp. is the common of intestinal micro flora in humans but nowadays this gram-positive bacterium causes the variety of nosocomial infections. Resistance to antibiotic and also, presence of different virulence genes in the enterococcus spp. can change it to problematic microorganisms in the health care centers. The aim of this study was determined the genotyping, antimicrobial resistance and virulence factor gene profiles of vancomycin resistance Enterococcus faecalis isolated from blood culture...
May 31, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28566074/-evaluation-of-virulence-factors-in-enterococcus-species
#8
Ergun Mete, İlknur Kaleli, Nural Cevahir, Melek Demir, Yüksel Akkaya, Özgün Kiriş Satılmış
Enterococci have recently become important due to their increased isolation rates in community-based and nosocomial infections and resistance to many antibiotics, including glycopeptides. The aim of this study was to evaluate the antimicrobial susceptible patterns and virulence factors of various clinical specimens; urine (n= 149), blood (n= 38), wound (n= 17), stool (n= 13), and other (n= 12) with a total of 229 enterococci including 138 E.faecalis and 91 E.faecium isolates. Aggregation factor (AF), enterococcus surface protein (esp), cytolysins and gelatinase encoding genes (asa1, esp, cylM, cylBcyl A, cylll, cylls, gelE, respectively) were investigated by molecular methods...
April 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28553278/a-thiazole-orange-derivative-targeting-the-bacterial-protein-ftsz-shows-potent-antibacterial-activity
#9
Ning Sun, Yu-Jing Lu, Fung-Yi Chan, Ruo-Lan Du, Yuan-Yuan Zheng, Kun Zhang, Lok-Yan So, Ruben Abagyan, Chao Zhuo, Yun-Chung Leung, Kwok-Yin Wong
The prevalence of multidrug resistance among clinically significant bacteria calls for the urgent development of new antibiotics with novel mechanisms of action. In this study, a new small molecule exhibiting excellent inhibition of bacterial cell division with potent antibacterial activity was discovered through cell-based screening. The compound exhibits a broad spectrum of bactericidal activity, including the methicillin-resistant Staphylococcus aureus, vancomycin-resistant Enterococcus and NDM-1 Escherichia coli...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28551912/-bacteria-without-borders-a-high-carriage-rate-of-antibiotic-resistant-bacteria-among-syrian-children-hospitalized-in-galilee-medical-center
#10
Diana Faour Kassem, Naama Shahar, Smadar Ocampo, Tarif Bader, Zeev Zonis, Daniel Glikman
INTRODUCTION: As the civil war in Syria enters its fifth year, the Israeli government continues to provide humanitarian aid to Syrian civilians in Israeli hospitals. Many wounded Syrian children are treated at the Galilee Medical Center (GMC). Due to the patients' incomplete medical history and increasing infection rates in Syria, contact isolation and screening cultures for multi-drug resistant bacteria (MDR's) are conducted upon admission for all Syrian children. AIMS: To describe the rate of MDR carriage in Syrian children and compare it to hospitalized Israeli children...
May 2017: Harefuah
https://www.readbyqxmd.com/read/28544183/clinical-outcomes-of-intestinal-transplant-recipients-colonized-with-multi-drug-resistant-organisms-a-retrospective-study
#11
Jacques Simkins, Michele I Morris, Jose F Camargo, Rodrigo Vianna, Thiago Beduschi, Lilian M Abbo
Rates of multi-drug resistant organisms (MDRO) colonization among intestinal transplant (ITx) recipients have not been reported. Colonization rates with vancomycin-resistant Enterococcus (VRE), carbapenem-resistant gram-negative bacteria (CR-GNB) and methicillin-resistant Staphylococcus aureus (MRSA) were obtained retrospectively in adults undergoing ITx (isolated or multi-visceral) from 1/2009-12/2015. We assessed for VRE, CR-GNB and MRSA bacteremia during the first year post-transplant for patients colonized with VRE, CR-GNB and MRSA respectively and for those who were not colonized...
May 19, 2017: Transplant International: Official Journal of the European Society for Organ Transplantation
https://www.readbyqxmd.com/read/28541565/emergence-of-vana-enterococcus-faecium-in-denmark-2005-15
#12
Anette M Hammerum, Sharmin Baig, Yasmin Kamel, Louise Roer, Mette Pinholt, Heidi Gumpert, Barbara Holzknecht, Bent Røder, Ulrik S Justesen, Jurgita Samulioniené, Mona Kjærsgaard, Claus Østergaard, Anette Holm, Esad Dzajic, Turid Snekloth Søndergaard, Shahin Gaini, Petra Edquist, Erik Alm, Berit Lilje, Henrik Westh, Marc Stegger, Henrik Hasman
Objectives: To describe the changing epidemiology of vancomycin-resistant Enterococcus faecium and Enterococcus faecalis in clinical samples in Denmark 2005-15 according to species and van type, and, furthermore, to investigate the genetic relatedness of the clinical E. faecium isolates from 2015. Methods: During 2005-14, all clinical VRE isolates were tested for the presence of vanA/B/C genes by PCR. In 2015, all clinical VRE isolates were whole-genome sequenced...
May 25, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28536572/the-bacteriophage-ef-p29-efficiently-protects-against-lethal-vancomycin-resistant-enterococcus-faecalis-and-alleviates-gut-microbiota-imbalance-in-a-murine-bacteremia-model
#13
Mengjun Cheng, Jiaming Liang, Yufeng Zhang, Liyuan Hu, Pengjuan Gong, Ruopeng Cai, Lei Zhang, Hao Zhang, Jinli Ge, Yalu Ji, Zhimin Guo, Xin Feng, Changjiang Sun, Yongjun Yang, Liancheng Lei, Wenyu Han, Jingmin Gu
Enterococcus faecalis is becoming an increasingly important opportunistic pathogen worldwide, especially because it can cause life-threatening nosocomial infections. Treating E. faecalis infections has become increasingly difficult because of the prevalence of multidrug-resistant E. faecalis strains. Because bacteriophages show specificity for their bacterial hosts, there has been a growth in interest in using phage therapies to combat the rising incidence of multidrug-resistant bacterial infections. In this study, we isolated a new lytic phage, EF-P29, which showed high efficiency and a broad host range against E...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28511382/rapid-identification-of-vancomycin-resistant-enterococcus-faecalis-clinical-isolates-using-a-sugar-fermentation-method
#14
Javad Raeisi, Mahnaz Saifi, Mohammad Reza Pourshafie, Mehri Habibi, Hamid Reza Mohajerani, Neda Akbari, Mohammad Reza Asadi Karam
INTRODUCTION: Vancomycin Resistant Enterococci (VRE) can be found all over the world. Thus, rapid detection of the isolates could be of high importance in the treatment or prevention of the associated disease. AIM: To measure the turanose fermentation in Enterococcus faecalis clinical isolates for rapid differentiation of VRE and Vancomycin-Susceptible E. faecalis (VSE) isolates. MATERIALS AND METHODS: Forty E. faecalis samples were isolated from 200 clinical samples in Tehran Medical Center, Iran, from October 2012 to December 2012...
March 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28504889/genetic-characterization-of-vana-enterococcus-faecium-isolates-from-wild-red-legged-partridges-in-portugal
#15
Vanessa Silva, Gilberto Igrejas, Isabel Carvalho, Fernando Peixoto, Lucas Cardoso, José Eduardo Pereira, Rosa Del Campo, Patricia Poeta
Vancomycin-resistant enterococci (VRE) have been detected in wild animals representing a public health concern. The red-legged partridge (Alectoris rufa) is a common game bird and its meat is consumed in several countries, including Portugal. Three hundred five fecal samples of red-legged partridge from the north of Portugal were screened for VRE. Samples were cultured on Slanetz-Bartley agar supplemented with vancomycin (4 mg/L) and six vanA-Enterococcus faecium were recovered. Isolates were tested for antibiotic resistance and virulence genes...
May 15, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28500123/enterococcus-casseliflavus-septicaemia-associated-with-hepatobiliary-infection-in-a-75-year-old-man
#16
Rajanshu Verma, Allison L Baroco
Enterococcus casseliflavus is a rare non-faecium, non-faecalis, vancomycin-resistant enterococcus (VRE) that is responsible for up to 2% of all enterococcal infections. Septicaemia with this bacterium is usually seen in older patients with multiple comorbidities who have had several previous hospitalisations. Septicaemia with E. casseliflavus portends a poorer prognosis, and treatment usually involves administration of antienterococcal beta-lactams or anti-VRE medications such as linezolid or daptomycin. We present such a case of a 75-year-old man who developed E...
May 12, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28494240/cooperating-commensals-restore-colonization-resistance-to-vancomycin-resistant-enterococcus-faecium
#17
Silvia Caballero, Sohn Kim, Rebecca A Carter, Ingrid M Leiner, Bože Sušac, Liza Miller, Grace J Kim, Lilan Ling, Eric G Pamer
Antibiotic-mediated microbiota destruction and the consequent loss of colonization resistance can result in intestinal domination with vancomycin-resistant Enterococcus (VRE), leading to bloodstream infection in hospitalized patients. Clearance of VRE remains a challenging goal that, if achieved, would reduce systemic VRE infections and patient-to-patient transmission. Although obligate anaerobic commensal bacteria have been associated with colonization resistance to VRE, the specific bacterial species involved remain undefined...
May 10, 2017: Cell Host & Microbe
https://www.readbyqxmd.com/read/28494236/allied-commensal-forces-against-vancomycin-resistant-enterococci
#18
Brayon J Fremin, Ami S Bhatt
In this issue of Cell Host & Microbe, Caballero et al. (2017) define a precise, limited consortium of commensal bacteria that restores resistance to colonization by clinically vexing vancomycin-resistant Enterococcus species.
May 10, 2017: Cell Host & Microbe
https://www.readbyqxmd.com/read/28490489/a-single-negative-result-for-van-quantitative-pcr-on-enrichment-broth-can-replace-five-rectal-swab-cultures-in-screening-for-vancomycin-resistant-enterococci
#19
J M Fonville, C M C van Herk, P H A C Das, J H B van de Bovenkamp, L van Dommelen
The increased incidence of infections by vancomycin-resistant Enterococcus (VRE) causes an accumulation of patients who are either colonized with VRE or flagged as potentially colonized with VRE. Since such patients require precautionary isolation upon admission to a hospital, rapid methods to establish VRE colonization status would improve patient care and optimize hospital operation. We evaluated van quantitative PCR (qPCR) on one enrichment broth as a VRE-screening approach. We obtained 255 sets of five rectal specimens from 243 patients...
July 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28484708/activity-of-fosfomycin-and-daptomycin-containing-bone-cement-on-selected-bacterial-species-being-associated-with-orthopedic-infections
#20
Sigrun Eick, Kevin Hofpeter, Anton Sculean, Claudia Ender, Susann Klimas, Sebastian Vogt, Sandor Nietzsche
The purpose of this study was to determine activity of fosfomycin/gentamicin and daptomycin/gentamicin-containing PMMA bone-cement against Staphylococcus aureus (MRSA, MSSA), Staphylococcus epidermidis, Enterococcus faecium (VRE), and E. coli (ESBL; only fosfomycin). Test specimens of the bone cement were formed and bacteria in two concentrations were added one time or repeatedly up to 96 h. All fosfomycin-containing cement killed ultimately all MSSA, Staphylococcus epidermidis, and E. coli within 24 h; growth of MRSA was suppressed up to 48 h...
2017: BioMed Research International
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